##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1295097_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2423787
Sequences flagged as poor quality	0
Sequence length	100
%GC	44
>>END_MODULE
>>Per base sequence quality	warn
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	26.554931188260355	33.0	27.0	33.0	6.0	33.0
2	22.627061288801368	27.0	6.0	33.0	2.0	33.0
3	25.04940574398658	33.0	15.0	33.0	15.0	33.0
4	25.096935498045003	33.0	6.0	37.0	2.0	37.0
5	26.947565111950844	33.0	15.0	37.0	2.0	37.0
6	27.696368534033724	33.0	15.0	37.0	2.0	37.0
7	27.71463787865848	33.0	15.0	37.0	6.0	37.0
8	28.607029000485603	33.0	22.0	37.0	6.0	37.0
9	29.552820029152727	37.0	27.0	37.0	15.0	37.0
10-11	29.048676306952714	35.0	24.5	37.0	10.5	37.0
12-13	27.87898338426603	33.0	15.0	37.0	4.0	37.0
14-15	29.653146501734682	33.0	27.0	37.0	10.5	40.0
16-17	30.051585597249264	37.0	27.0	40.0	6.0	40.0
18-19	30.19902202627541	37.0	27.0	40.0	10.5	40.0
20-21	29.691849778879085	33.0	27.0	40.0	6.0	40.0
22-23	30.454432464568875	37.0	27.0	40.0	15.0	40.0
24-25	30.583854315581362	37.0	27.0	40.0	15.0	40.0
26-27	29.14169252496197	33.0	22.0	40.0	4.0	40.0
28-29	29.85535713327945	33.0	27.0	40.0	10.5	40.0
30-31	30.56132160127932	37.0	27.0	40.0	15.0	40.0
32-33	29.346799038034284	33.0	27.0	38.5	6.0	40.0
34-35	28.95589670214421	33.0	22.0	37.0	6.0	40.0
36-37	29.28309562680219	33.0	22.0	37.0	6.0	40.0
38-39	29.373268154338646	33.0	24.5	37.0	6.0	40.0
40-41	28.384102027117066	33.0	22.0	37.0	6.0	40.0
42-43	29.025627870765874	33.0	22.0	37.0	6.0	40.0
44-45	28.844425479631667	33.0	22.0	37.0	6.0	40.0
46-47	28.547489734040163	33.0	22.0	37.0	6.0	40.0
48-49	28.479317902109386	33.0	22.0	37.0	6.0	40.0
50-51	28.4394948896087	33.0	22.0	37.0	6.0	40.0
52-53	28.606082135105105	33.0	22.0	37.0	6.0	40.0
54-55	28.564986733570237	33.0	22.0	37.0	6.0	40.0
56-57	28.560984112877904	33.0	22.0	37.0	6.0	40.0
58-59	28.396148052613533	33.0	22.0	37.0	6.0	40.0
60-61	28.349218186251512	33.0	22.0	37.0	10.5	40.0
62-63	28.285633184764173	33.0	22.0	37.0	10.5	40.0
64-65	27.838076324363485	33.0	22.0	37.0	6.0	40.0
66-67	28.022671134055923	33.0	22.0	37.0	10.5	38.5
68-69	27.599586308532885	33.0	22.0	37.0	6.0	37.0
70-71	27.725444727610142	33.0	22.0	37.0	10.5	37.0
72-73	27.784762646222624	33.0	22.0	37.0	10.5	37.0
74-75	27.874617901655547	33.0	22.0	37.0	15.0	37.0
76-77	27.644755087802682	33.0	22.0	37.0	15.0	37.0
78-79	27.17872692608715	30.0	22.0	33.0	10.5	37.0
80-81	26.989671947246187	27.0	22.0	33.0	6.0	37.0
82-83	27.11958806611307	27.0	22.0	33.0	15.0	37.0
84-85	27.08037566832399	27.0	22.0	33.0	10.5	37.0
86-87	26.878492623320447	27.0	22.0	33.0	15.0	37.0
88-89	26.828181890570416	27.0	22.0	33.0	15.0	37.0
90-91	26.992513781120206	27.0	22.0	33.0	15.0	37.0
92-93	26.916474302403635	27.0	22.0	33.0	15.0	37.0
94-95	26.785907755095643	27.0	22.0	33.0	15.0	37.0
96-97	26.894682989883187	27.0	22.0	33.0	15.0	37.0
98-99	26.965761430356707	27.0	22.0	33.0	15.0	37.0
100	26.63788856033967	27.0	22.0	33.0	15.0	37.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	7.44349500541594
1101	2	11.353935438594053
1101	3	8.567354545905392
1101	4	13.028586908256408
1101	5	10.917928183047305
1101	6	10.035531967955848
1101	7	9.60863975338982
1101	8	9.078985626862451
1101	9	7.459266724954627
1101	10-11	7.920689410406478
1101	12-13	9.293296618944364
1101	14-15	8.098245222622811
1101	16-17	8.335876569418755
1101	18-19	8.293453651624507
1101	20-21	7.3379998964059325
1101	22-23	7.4171996361807615
1101	24-25	7.556786418475319
1101	26-27	8.752432951625345
1101	28-29	8.391354510626115
1101	30-31	5.665958022346558
1101	32-33	7.229343566806325
1101	34-35	8.823714848918225
1101	36-37	8.36103996886423
1101	38-39	8.284174303117553
1101	40-41	9.163999495631423
1101	42-43	8.78304375406034
1101	44-45	8.666550798555484
1101	46-47	7.5156394486244515
1101	48-49	5.49379793051099
1101	50-51	7.002812748791719
1101	52-53	6.030849932869149
1101	54-55	4.211480595275017
1101	56-57	6.379467933101061
1101	58-59	6.4218896488111135
1101	60-61	6.942027813633967
1101	62-63	6.360102328429793
1101	64-65	6.952948359183804
1101	66-67	6.0284679382856226
1101	68-69	6.923773508467576
1101	70-71	6.376391012719171
1101	72-73	5.607415122509082
1101	74-75	5.059311995410962
1101	76-77	5.199151208278035
1101	78-79	5.271772136926174
1101	80-81	5.169337068373046
1101	82-83	4.713826570129786
1101	84-85	4.449160320261882
1101	86-87	4.217604855361035
1101	88-89	4.186494753106604
1101	90-91	3.968887389170586
1101	92-93	3.8874094279265066
1101	94-95	3.711374571093728
1101	96-97	3.863943678067489
1101	98-99	3.618311302373858
1101	100	3.757970268817072
1102	1	6.8926112606882555
1102	2	10.5779749381242
1102	3	8.234960589650633
1102	4	12.731370999039356
1102	5	10.697267877044187
1102	6	9.474387207510617
1102	7	6.938222346392841
1102	8	7.964197144982851
1102	9	6.751512713742411
1102	10-11	7.613432874664614
1102	12-13	9.169704672945574
1102	14-15	6.286208431145692
1102	16-17	7.546814111382947
1102	18-19	7.645078350941599
1102	20-21	7.196048665853944
1102	22-23	6.7766605866678375
1102	24-25	7.194598203204158
1102	26-27	8.735616194469422
1102	28-29	7.871252989433984
1102	30-31	4.285537126794026
1102	32-33	4.410542640860168
1102	34-35	5.213801768453486
1102	36-37	5.438159803504835
1102	38-39	4.639690808081916
1102	40-41	6.540027432436922
1102	42-43	7.276742259639345
1102	44-45	6.638536429803203
1102	46-47	5.625502843492487
1102	48-49	3.7297460022407343
1102	50-51	5.183577167874173
1102	52-53	5.952307765213508
1102	54-55	5.603618464623416
1102	56-57	6.580131138236361
1102	58-59	6.240511384324176
1102	60-61	6.227955298517191
1102	62-63	4.4528666803337345
1102	64-65	6.346002556268498
1102	66-67	3.9103113459003893
1102	68-69	6.265996332129227
1102	70-71	5.580217444654487
1102	72-73	5.158697408388868
1102	74-75	4.454708308857857
1102	76-77	3.5113632332452376
1102	78-79	4.575078909443633
1102	80-81	4.73723137094089
1102	82-83	4.229891418588895
1102	84-85	3.1772689537815637
1102	86-87	3.434446460686729
1102	88-89	3.555930458655329
1102	90-91	3.515206164510687
1102	92-93	3.3579454035571814
1102	94-95	3.1982798291297385
1102	96-97	3.4494404326938373
1102	98-99	3.2314992992790614
1102	100	3.464851281714271
1103	1	6.744590208700675
1103	2	10.923422314658335
1103	3	8.012882291155083
1103	4	12.02135542141722
1103	5	10.403185164586287
1103	6	9.201225112975465
1103	7	8.09795059285278
1103	8	8.04484797845721
1103	9	7.163662924773082
1103	10-11	7.475762178577629
1103	12-13	8.765282129418285
1103	14-15	8.695698588266854
1103	16-17	8.143815489965082
1103	18-19	7.3858937917590985
1103	20-21	8.440145441695243
1103	22-23	6.896674833881555
1103	24-25	5.299551278878091
1103	26-27	5.946297266942821
1103	28-29	7.046094450308168
1103	30-31	6.216459684017757
1103	32-33	8.037552340489402
1103	34-35	7.312988457306705
1103	36-37	4.196882407245628
1103	38-39	6.271017014562901
1103	40-41	6.7806257282207945
1103	42-43	7.624764968279681
1103	44-45	5.657673820884639
1103	46-47	6.458537749238754
1103	48-49	5.730395293865463
1103	50-51	6.573404182808964
1103	52-53	6.33908920933661
1103	54-55	5.063268089104557
1103	56-57	5.90804680531048
1103	58-59	5.495085562440941
1103	60-61	5.121163974058916
1103	62-63	2.8657423672179334
1103	64-65	5.097442790594762
1103	66-67	4.195528477491877
1103	68-69	5.208128906347451
1103	70-71	3.9403267067166787
1103	72-73	4.565664603249232
1103	74-75	3.320804717619776
1103	76-77	3.643009489780386
1103	78-79	3.0617101576493795
1103	80-81	4.217560617597666
1103	82-83	3.4805260328514684
1103	84-85	3.313460160864537
1103	86-87	2.8475769850878088
1103	88-89	3.3025350652689625
1103	90-91	2.8594453289299118
1103	92-93	2.4887466945256413
1103	94-95	2.969006589615752
1103	96-97	3.2297815011665634
1103	98-99	2.911858049613574
1103	100	3.0631791450659946
1104	1	6.647815073152575
1104	2	9.969348601250957
1104	3	7.656879204452082
1104	4	12.485391761906278
1104	5	9.82798435116445
1104	6	8.386646870988628
1104	7	4.2944903520955044
1104	8	6.818837442243719
1104	9	6.824825053664295
1104	10-11	7.447036859374162
1104	12-13	7.760842188136063
1104	14-15	7.55641742117513
1104	16-17	7.5024965831660175
1104	18-19	7.169645090787899
1104	20-21	7.876129760301126
1104	22-23	6.771532610238523
1104	24-25	6.309349785515295
1104	26-27	8.578529643925961
1104	28-29	7.499966095696607
1104	30-31	6.664329794762743
1104	32-33	8.040353385710493
1104	34-35	8.316040647157624
1104	36-37	6.917426015445894
1104	38-39	7.645560081287691
1104	40-41	7.515627118318143
1104	42-43	7.58352344300339
1104	44-45	7.1636430193902
1104	46-47	6.958087689504165
1104	48-49	5.807413912461897
1104	50-51	6.082775094225255
1104	52-53	6.234283169674217
1104	54-55	5.031788736882792
1104	56-57	6.1577060184504475
1104	58-59	4.085359332608284
1104	60-61	4.6639256543955305
1104	62-63	4.119292407851116
1104	64-65	2.9798142993006174
1104	66-67	3.412619194227723
1104	68-69	4.44615790107191
1104	70-71	2.81923284367274
1104	72-73	2.757502447820997
1104	74-75	3.4513806698402156
1104	76-77	4.145984564604859
1104	78-79	4.578059744368012
1104	80-81	3.371058631393094
1104	82-83	3.208007478608266
1104	84-85	3.262634771092088
1104	86-87	0.8946931589783809
1104	88-89	0.5546571360474566
1104	90-91	2.5029372129346505
1104	92-93	2.657178843005081
1104	94-95	2.63148590471571
1104	96-97	3.100670302799802
1104	98-99	2.8891775050197097
1104	100	1.1638884043755091
1105	1	5.966024410657532
1105	2	-2.736018329380453
1105	3	0.44599174928256247
1105	4	8.765808198909077
1105	5	9.029835711010573
1105	6	8.328111957454595
1105	7	5.765338496741915
1105	8	6.401757399500273
1105	9	5.194008949605649
1105	10-11	5.247951096230164
1105	12-13	5.468250705441246
1105	14-15	6.220680722749226
1105	16-17	6.381716313279579
1105	18-19	6.489345293760717
1105	20-21	7.391038735989895
1105	22-23	2.3212810421512344
1105	24-25	4.420579154241317
1105	26-27	7.331819780625295
1105	28-29	6.421457270844833
1105	30-31	5.740166512392598
1105	32-33	7.540682364446969
1105	34-35	6.547687242193721
1105	36-37	6.653052607396507
1105	38-39	4.443377231272876
1105	40-41	5.851404830509146
1105	42-43	5.994859500390934
1105	44-45	6.413992174557627
1105	46-47	5.647631690835038
1105	48-49	5.321271202309312
1105	50-51	6.223834971489865
1105	52-53	5.927506645787194
1105	54-55	5.543924921465884
1105	56-57	5.382515475333406
1105	58-59	2.0157356150201586
1105	60-61	4.632527861094712
1105	62-63	-0.4833506573953237
1105	64-65	-0.06605417756106746
1105	66-67	0.9506308159274752
1105	68-69	2.9276010040087215
1105	70-71	3.101908266740491
1105	72-73	2.9285720139267077
1105	74-75	2.9085821072858202
1105	76-77	2.0436806182384757
1105	78-79	3.3664346456809966
1105	80-81	1.8672372890155806
1105	82-83	1.0505017990892966
1105	84-85	1.4985881324741541
1105	86-87	-0.2873458898270549
1105	88-89	1.713131227602947
1105	90-91	1.4457533177486113
1105	92-93	1.6902525169075027
1105	94-95	1.0922301421589715
1105	96-97	2.173135979735587
1105	98-99	2.1038232712831686
1105	100	2.159822490043293
1106	1	6.197629813325037
1106	2	10.461712388477896
1106	3	6.897934648606267
1106	4	11.93752389424099
1106	5	10.128378593971473
1106	6	9.493766796018967
1106	7	6.694174080880288
1106	8	7.527028282071402
1106	9	7.077242313550297
1106	10-11	5.819696207841968
1106	12-13	8.09112158727697
1106	14-15	8.017700393967143
1106	16-17	8.00341936880373
1106	18-19	7.301704176052393
1106	20-21	8.46648781686904
1106	22-23	6.441979072087811
1106	24-25	5.235093790219452
1106	26-27	7.915481151442322
1106	28-29	6.465984142156884
1106	30-31	5.611748271725141
1106	32-33	7.547305899493249
1106	34-35	6.87706390203029
1106	36-37	6.169456228172859
1106	38-39	7.27328963510692
1106	40-41	6.904589556473518
1106	42-43	6.269290226430687
1106	44-45	4.581165996274748
1106	46-47	6.2283876524578226
1106	48-49	6.0159444655767444
1106	50-51	6.674407623726005
1106	52-53	5.692679057361399
1106	54-55	5.305292337236505
1106	56-57	5.035417161529722
1106	58-59	5.206915695050824
1106	60-61	5.090742035513028
1106	62-63	5.099077527813247
1106	64-65	5.422818471089965
1106	66-67	3.039761684360407
1106	68-69	3.418269424621073
1106	70-71	2.951601004504468
1106	72-73	2.768754192625096
1106	74-75	2.9517262295913547
1106	76-77	3.575507746115644
1106	78-79	4.229210457777157
1106	80-81	3.840408262869108
1106	82-83	2.181288969546525
1106	84-85	2.8128040093596667
1106	86-87	3.4554291500917564
1106	88-89	3.577795472942075
1106	90-91	3.1873168665653893
1106	92-93	2.996610937731287
1106	94-95	1.6048639747417326
1106	96-97	1.670458362858728
1106	98-99	2.0899020168259135
1106	100	3.000473214924778
1107	1	5.962406190202447
1107	2	10.422548093249848
1107	3	6.197667501008578
1107	4	11.29746166507779
1107	5	9.484797814222848
1107	6	8.086486179956786
1107	7	3.7235218726380275
1107	8	6.366624064776836
1107	9	5.627616352859004
1107	10-11	4.5295395567598185
1107	12-13	6.123046160826419
1107	14-15	6.039965997270929
1107	16-17	6.950434187708584
1107	18-19	6.1460576472831185
1107	20-21	5.388242213151408
1107	22-23	5.547505531461887
1107	24-25	4.507606121155753
1107	26-27	7.155194008614735
1107	28-29	6.373436449286231
1107	30-31	5.072900948809803
1107	32-33	4.877658190177716
1107	34-35	6.736252505184876
1107	36-37	6.554133413027447
1107	38-39	6.3526851279033
1107	40-41	7.632779672339119
1107	42-43	6.12025481113216
1107	44-45	4.106549102047985
1107	46-47	3.459565127548192
1107	48-49	3.9080664974667414
1107	50-51	4.66011920233634
1107	52-53	3.7573118410437267
1107	54-55	5.089206251019601
1107	56-57	5.1693170808300195
1107	58-59	3.040875567368868
1107	60-61	2.7521013626452238
1107	62-63	0.28160930944704177
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2103	10-11	7.755096340651821
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2103	78-79	3.516079098912421
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2104	3	8.010275503227863
2104	4	12.517089312989505
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2207	9	6.148607499234256
2207	10-11	7.040244943352608
2207	12-13	8.883222084330487
2207	14-15	8.366148514575602
2207	16-17	7.493932551515751
2207	18-19	4.044045271862821
2207	20-21	7.606323342990997
2207	22-23	6.529350516607074
2207	24-25	4.466590345020954
2207	26-27	4.03880688353696
2207	28-29	5.282959974074693
2207	30-31	5.680166973022175
2207	32-33	7.091892065881627
2207	34-35	6.473113347043448
2207	36-37	6.909158585306802
2207	38-39	7.110062970882936
2207	40-41	4.0037042987518845
2207	42-43	5.717608177835256
2207	44-45	4.063281122632617
2207	46-47	5.715338966497189
2207	48-49	2.4534437491104484
2207	50-51	5.389534945813363
2207	52-53	3.082901796689267
2207	54-55	3.4481911250558817
2207	56-57	5.419582265362429
2207	58-59	3.8017679047935893
2207	60-61	3.4124899347267075
2207	62-63	4.358011179552239
2207	64-65	3.4580168666340008
2207	66-67	3.691775959047426
2207	68-69	3.7638740177191075
2207	70-71	3.8417464993911743
2207	72-73	3.9163896931417526
2207	74-75	3.621530134774609
2207	76-77	4.185673103858647
2207	78-79	4.379956948699331
2207	80-81	4.295988959472545
2207	82-83	3.9732411379886905
2207	84-85	3.3549617433655996
2207	86-87	3.5594376651932613
2207	88-89	3.345710531911937
2207	90-91	1.491192266246447
2207	92-93	-0.6635561609578602
2207	94-95	1.4682830001225824
2207	96-97	-0.7999181796865109
2207	98-99	0.7969847214098529
2207	100	2.23030392532187
2208	1	0.9145694436512457
2208	2	8.658362351241234
2208	3	6.728122956715442
2208	4	11.887851661009059
2208	5	10.086363859193273
2208	6	9.247909953098777
2208	7	7.553090578193778
2208	8	7.807634082575465
2208	9	6.473776043233176
2208	10-11	6.739776866236628
2208	12-13	7.077887057321444
2208	14-15	7.847168372542388
2208	16-17	7.208410606617658
2208	18-19	6.489247934388562
2208	20-21	6.618832937604175
2208	22-23	5.764460232861829
2208	24-25	6.1991101848691486
2208	26-27	8.033387883073289
2208	28-29	7.014054725615388
2208	30-31	5.960246445656118
2208	32-33	8.347625619355743
2208	34-35	6.417926614458086
2208	36-37	6.959678238615954
2208	38-39	7.250637385549766
2208	40-41	7.542719424062476
2208	42-43	7.020433781122296
2208	44-45	5.893802215133746
2208	46-47	4.574516038995402
2208	48-49	4.616553768851464
2208	50-51	5.8760477198260475
2208	52-53	5.520657464305511
2208	54-55	6.275532303323796
2208	56-57	6.2661191041474
2208	58-59	5.681821393732601
2208	60-61	5.027409259677725
2208	62-63	3.3041627513804777
2208	64-65	3.3611522462141927
2208	66-67	2.884575953364781
2208	68-69	5.477274017142605
2208	70-71	5.29729554186525
2208	72-73	4.485358850784127
2208	74-75	2.009731375403387
2208	76-77	3.2815780055941595
2208	78-79	4.071426102223846
2208	80-81	3.4568511940044253
2208	82-83	1.3761567268055686
2208	84-85	1.877574867149285
2208	86-87	1.2049086783432337
2208	88-89	0.6321032497240715
2208	90-91	1.7851074387477084
2208	92-93	0.7453183312070877
2208	94-95	-1.1953963137266825
2208	96-97	1.4133232821372204
2208	98-99	0.3146617667301932
2208	100	1.9163664319619187
2209	1	5.354809209007428
2209	2	9.639098339937924
2209	3	5.610364139339435
2209	4	-3.35878749847058
2209	5	0.5895154295630114
2209	6	4.3339858411498575
2209	7	2.332774147575133
2209	8	5.286928855774519
2209	9	4.97865062265188
2209	10-11	5.1940510661933
2209	12-13	7.298050753386075
2209	14-15	6.637885567745446
2209	16-17	6.313106299516843
2209	18-19	5.3094641248771985
2209	20-21	6.001185898790418
2209	22-23	5.5876227375289105
2209	24-25	5.268023131207819
2209	26-27	6.798792642567726
2209	28-29	6.167184348471935
2209	30-31	3.9755345281972687
2209	32-33	6.253247298654816
2209	34-35	5.787529948194173
2209	36-37	6.328916506153011
2209	38-39	6.794995922193536
2209	40-41	7.182072594246726
2209	42-43	6.846692409093851
2209	44-45	6.494552526181579
2209	46-47	6.70743177088578
2209	48-49	5.007084866014861
2209	50-51	4.353965228743839
2209	52-53	4.809049420494301
2209	54-55	5.413886201735519
2209	56-57	4.960619653311461
2209	58-59	3.066765875912804
2209	60-61	4.008841296134815
2209	62-63	4.056337637003047
2209	64-65	5.051569578245644
2209	66-67	4.491309649070196
2209	68-69	3.7360192099160656
2209	70-71	3.616360814558682
2209	72-73	0.7473942656070953
2209	74-75	2.1977674090564214
2209	76-77	3.3691189887992117
2209	78-79	3.58738955042989
2209	80-81	3.4995670688189655
2209	82-83	3.0392149120714294
2209	84-85	2.9958867945137655
2209	86-87	1.5060839384205842
2209	88-89	2.045087686991465
2209	90-91	2.360623688343594
2209	92-93	2.231741828135796
2209	94-95	0.6501955833548543
2209	96-97	0.19277329351237427
2209	98-99	0.21121563977913738
2209	100	0.03220997844057294
2210	1	1.8976671234871674
2210	2	-17.208010629669193
2210	3	-7.811641180067086
2210	4	-6.871836434775531
2210	5	-0.7892399112626869
2210	6	2.9299027868866965
2210	7	-7.144859573290127
2210	8	-7.1720683168938635
2210	9	-2.7849747689465865
2210	10-11	-0.7095146683355331
2210	12-13	3.528579322845683
2210	14-15	-4.346336878809044
2210	16-17	-0.7880490323686757
2210	18-19	-0.2550611516164629
2210	20-21	2.680829949287528
2210	22-23	2.698530314703145
2210	24-25	2.2863914740338913
2210	26-27	4.272802797121074
2210	28-29	3.5972651639652646
2210	30-31	2.785902155809012
2210	32-33	4.6758560801456674
2210	34-35	2.519880079130541
2210	36-37	4.2148328372525405
2210	38-39	3.253617327962189
2210	40-41	4.092823997668582
2210	42-43	2.7759239075796067
2210	44-45	3.4617499849356896
2210	46-47	2.889888718336149
2210	48-49	2.9819168651506445
2210	50-51	2.2865469661931286
2210	52-53	2.4702063914126455
2210	54-55	2.918611458858795
2210	56-57	2.874610009093331
2210	58-59	1.9954870391790962
2210	60-61	2.950848403577144
2210	62-63	1.9688291158949127
2210	64-65	2.844134143101055
2210	66-67	2.8017182525024786
2210	68-69	3.502601379474008
2210	70-71	2.4505326000986862
2210	72-73	1.093944008390693
2210	74-75	0.3672881037083364
2210	76-77	-0.31995089732525983
2210	78-79	0.3710050892060579
2210	80-81	1.039686587837334
2210	82-83	0.9148593499762967
2210	84-85	-0.8573299005255528
2210	86-87	-0.6586514322802124
2210	88-89	-1.471135478502422
2210	90-91	-1.4948671647357408
2210	92-93	-0.8976202342145037
2210	94-95	-0.11977919789013924
2210	96-97	0.21714878475658494
2210	98-99	-0.12166494177951037
2210	100	0.021654979576720024
2211	1	0.8149921042362784
2211	2	-17.208010629669193
2211	3	-8.144919354825369
2211	4	-19.21090017452692
2211	5	-21.298364481482206
2211	6	-13.250660602954921
2211	7	-11.407375225608543
2211	8	-19.491247702410174
2211	9	-15.871231623784354
2211	10-11	-9.919818926000197
2211	12-13	-22.57602263430851
2211	14-15	-18.070586620765212
2211	16-17	-16.217829923316195
2211	18-19	-19.292743508440175
2211	20-21	-8.603589352624923
2211	22-23	-4.200174024412085
2211	24-25	-2.2403267116780903
2211	26-27	-17.610369995112098
2211	28-29	-4.399539773624404
2211	30-31	-4.636261000447348
2211	32-33	-0.8901394775215685
2211	34-35	-5.305540631783131
2211	36-37	-6.466716443852565
2211	38-39	-2.700797093937716
2211	40-41	-5.311606105806405
2211	42-43	-1.8584882557053781
2211	44-45	-0.10846134184259171
2211	46-47	0.5249752478631535
2211	48-49	0.4239132934963763
2211	50-51	-4.445964742326176
2211	52-53	-0.9761292106779216
2211	54-55	-0.9388844510587511
2211	56-57	-6.509684308325152
2211	58-59	-2.130828048134507
2211	60-61	-1.3200416194303024
2211	62-63	-0.4476636881345506
2211	64-65	-3.4870586747375576
2211	66-67	-2.538902176142841
2211	68-69	-0.043890687287369445
2211	70-71	-0.4772980412309238
2211	72-73	-1.5587136675062538
2211	74-75	-1.9637361957114443
2211	76-77	-2.032315702253488
2211	78-79	-1.488577406411295
2211	80-81	-1.1866000034797572
2211	82-83	-1.655957355816291
2211	84-85	-1.780178891638549
2211	86-87	-2.199228912134071
2211	88-89	-2.7180728317802263
2211	90-91	-2.480325195457585
2211	92-93	-2.4113280958327934
2211	94-95	-2.503100204413208
2211	96-97	-3.3863679254830146
2211	98-99	-3.0617049766066486
2211	100	-2.555412121180911
2212	1	-21.42261851523275
2212	2	-17.208010629669193
2212	3	-20.205981301728023
2212	4	-19.21090017452692
2212	5	-21.298364481482206
2212	6	-21.991811045432797
2212	7	-22.623304429148366
2212	8	-23.178415844003094
2212	9	-24.512824544138336
2212	10-11	-11.615363262031257
2212	12-13	-22.57602263430851
2212	14-15	-24.031869806605922
2212	16-17	-24.41539629499761
2212	18-19	-19.00607772420802
2212	20-21	-24.134562803952356
2212	22-23	-19.180832950867472
2212	24-25	-25.692317862120568
2212	26-27	-17.679468399166254
2212	28-29	-18.578723773826106
2212	30-31	-6.26186988025977
2212	32-33	-17.748765905174544
2212	34-35	-14.096495678047912
2212	36-37	-18.13402665740172
2212	38-39	-24.179336916946568
2212	40-41	-10.68352136688096
2212	42-43	-14.349061964199441
2212	44-45	-17.569883195963556
2212	46-47	-17.526807900158932
2212	48-49	-18.094099085799186
2212	50-51	-9.273708483344254
2212	52-53	-2.405910114795109
2212	54-55	-23.937114539554326
2212	56-57	-23.868976343723382
2212	58-59	-12.828030100425057
2212	60-61	-6.374867275583391
2212	62-63	-3.3413952515563814
2212	64-65	-17.66184058735133
2212	66-67	-17.93219441324039
2212	68-69	-14.944096798776668
2212	70-71	-9.94897063281502
2212	72-73	-17.858988482825318
2212	74-75	-6.792153108199155
2212	76-77	-11.360174790068328
2212	78-79	-4.863222541675899
2212	80-81	-2.551240852633878
2212	82-83	-3.233012167605615
2212	84-85	-5.42912123084637
2212	86-87	-4.884354848144962
2212	88-89	-3.4779598801628495
2212	90-91	-4.354930621169203
2212	92-93	-5.545288587979126
2212	94-95	-3.9371873889428706
2212	96-97	-5.8239495506752625
2212	98-99	-3.972180734870019
2212	100	-3.9429483640268863
2213	1	-21.42261851523275
2213	2	-17.208010629669193
2213	3	-20.205981301728023
2213	4	-19.21090017452692
2213	5	-21.298364481482206
2213	6	-21.991811045432797
2213	7	-10.759779637788522
2213	8	-10.6865159912785
2213	9	-4.082780361516836
2213	10-11	-17.958388030313756
2213	12-13	-16.54705847132373
2213	14-15	-24.031869806605922
2213	16-17	-18.34445864158573
2213	18-19	-18.920554665746813
2213	20-21	-18.11198057518456
2213	22-23	-20.326074402809958
2213	24-25	-7.828547611752377
2213	26-27	-23.71899831369617
2213	28-29	-24.626088446190778
2213	30-31	-19.94119027349769
2213	32-33	-23.829962486371127
2213	34-35	-18.282771267674015
2213	36-37	-24.250123523498583
2213	38-39	-11.799120913019223
2213	40-41	-17.533227025864967
2213	42-43	-23.739019229156707
2213	44-45	-17.535631699083197
2213	46-47	-17.393139735562933
2213	48-49	-6.230222364963705
2213	50-51	-23.83246916710494
2213	52-53	-18.186481824490528
2213	54-55	-18.027688913633853
2213	56-57	-17.868976343723382
2213	58-59	-18.386055456130826
2213	60-61	-19.66800354938977
2213	62-63	-17.86661616466389
2213	64-65	-17.913479078680542
2213	66-67	-8.780789212866118
2213	68-69	-23.093669448349317
2213	70-71	-17.760939067206134
2213	72-73	-9.618433409796019
2213	74-75	-4.516113998397159
2213	76-77	-3.087691688976978
2213	78-79	-4.679047514717368
2213	80-81	-3.3482027204720346
2213	82-83	-3.21852746368792
2213	84-85	-3.1666269477335334
2213	86-87	-3.4252143323034154
2213	88-89	-2.852194131974418
2213	90-91	-3.0190477659564507
2213	92-93	-3.8493797812455774
2213	94-95	-3.838662870315261
2213	96-97	-3.60793041326437
2213	98-99	-3.9044733849461757
2213	100	-3.325683994185198
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	682.0
3	664.0
4	3690.0
5	11946.0
6	16928.0
7	21414.0
8	20117.0
9	20070.0
10	23361.0
11	27851.0
12	34038.0
13	44893.0
14	33399.0
15	36984.0
16	42047.0
17	45243.0
18	46171.0
19	47258.0
20	47091.0
21	46658.0
22	48664.0
23	53934.0
24	59081.0
25	65580.0
26	72816.0
27	78654.0
28	85094.0
29	90065.0
30	94805.0
31	104366.0
32	120381.0
33	146458.0
34	176134.0
35	203444.0
36	219096.0
37	181291.0
38	53365.0
39	54.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.46327329833713	20.11961479615443	13.663524315116799	24.753587590391646
2	15.170389394949597	21.244889249495568	39.483673812632205	24.101047542922636
3	18.118995011663504	25.945157336303986	30.106005450300984	25.82984220173153
4	12.960414447796397	17.613324209447573	38.12128820853808	31.304973134217946
5	13.189882949802575	37.76952906133178	35.48912600414405	13.551461984721602
6	30.683556894408852	37.32400214163512	18.33098356015182	13.6614574038042
7	26.369087884407712	31.718485863640584	23.89613836144736	18.016287890504348
8	24.231507047934628	36.6544093235907	21.238839321002516	17.87524430747216
9	24.79739031153156	17.48373837873104	21.483172946229544	36.23569836350786
10-11	23.43548194079399	27.252159614216886	29.001901435606044	20.310457009383075
12-13	24.375257422118214	24.74060784377507	28.534259648261166	22.349875085845554
14-15	21.787748341714277	26.419916900741757	25.95797702067497	25.83435773686899
16-17	21.655535135028366	29.625122216406314	26.809341508327773	21.910001140237547
18-19	21.767725337946388	28.917499089500094	28.092554111998897	21.222221460554625
20-21	22.570704434395967	28.260570738574852	27.55074125218207	21.61798357484711
22-23	22.20456231604315	28.046605796942085	27.531586857203816	22.217245029810947
24-25	22.248374121790203	28.256484046141633	27.224694547339844	22.270447284728316
26-27	22.169886515115287	27.94642014601777	27.43462960977947	22.449063729087477
28-29	22.177867903048522	27.88657539093356	27.8211063864705	22.114450319547416
30-31	22.476967349947227	28.16300284797901	27.445410500054034	21.914619302019727
32-33	21.861126115261314	28.068948882841934	27.676191187977654	22.393733813919095
34-35	21.854026032912724	27.913191257748192	27.59140437736165	22.641378331977428
36-37	22.47895659774021	28.08743230008352	27.197492427258986	22.236118674917286
38-39	22.35470534323725	27.9619306121335	27.306846784822252	22.376517259807
40-41	22.16794140569206	27.973317741746	27.27144731716272	22.58729353539922
42-43	22.38272180100011	27.976784148647187	27.407742195719948	22.232751854632752
44-45	22.5176498754159	27.7565546328005	27.489485969159432	22.23630952262417
46-47	22.26038350589019	27.915988257405683	27.744207563798295	22.079420672905837
48-49	22.340424617093895	27.768851208591062	27.629800246456437	22.260923927858606
50-51	22.247302672703658	27.810410361556354	27.65325230962656	22.289034656113426
52-53	22.01027544520906	27.791874300328573	27.483688850637723	22.71416140382465
54-55	22.228230711787763	28.073659634921178	27.342497029169284	22.355612624121775
56-57	22.438436209370543	27.68384264973009	27.3213743358993	22.556346805000068
58-59	22.263851857889623	27.399083228049705	27.65879953067454	22.678265383386137
60-61	22.394062263939777	27.818592752758438	27.43004962130091	22.35729536200088
62-63	22.077869219262094	27.868750452827136	27.541649441477688	22.51173088643309
64-65	22.031731118812075	27.72366844209883	27.657811566284135	22.58678887280496
66-67	22.26052745951515	27.66279496286483	27.3596022640705	22.717075313549522
68-69	22.71746669203504	27.60308478276297	27.24964004627261	22.429808478929374
70-71	22.469228168823037	27.684124967280923	27.326127894247694	22.52051896964835
72-73	22.5274476456914	27.71665022994636	27.341205348307522	22.41469677605472
74-75	22.59515245492231	27.630262011804867	27.189441788633516	22.585143744639304
76-77	22.50333322832786	27.72219557679401	27.370378568369308	22.40409262650882
78-79	22.599676921715826	27.6027881315488	27.462662855180696	22.33487209155468
80-81	22.461117207380312	27.590183583620288	27.44842334725055	22.500275861748854
82-83	22.249621079146625	27.699363945368848	27.40843508599132	22.642579889493202
84-85	22.36049230622646	27.712733470500638	27.117106707372496	22.809667515900408
86-87	22.525949076491347	27.557944097185967	27.29118079486555	22.624926031457143
88-89	22.49426338356973	27.30780985410765	27.51495506628564	22.68297169603698
90-91	22.585242311153458	27.43256130564819	27.336905636281827	22.645290746916523
92-93	22.307231573779802	27.595785052059746	27.404053515947414	22.69292985821304
94-95	22.143281877682416	27.452719129825343	27.535523155042913	22.868475837449328
96-97	22.452297531019187	27.422297707397192	27.426925036235307	22.69847972534832
98-99	22.510395331417385	27.35003301717266	27.356458204570504	22.78311344683945
100	22.353442679725376	27.21537052403167	27.50891807057321	22.92226872566975
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	404.0
1	462.5
2	449.0
3	370.0
4	395.5
5	486.0
6	662.0
7	984.0
8	1383.5
9	1884.5
10	2580.5
11	3293.0
12	4034.5
13	4854.5
14	5557.5
15	6317.5
16	7072.0
17	7780.0
18	8603.5
19	9367.5
20	10101.5
21	11065.0
22	12245.5
23	13828.0
24	15796.5
25	18348.0
26	21756.0
27	26050.5
28	31575.5
29	37557.0
30	43131.5
31	48815.0
32	54376.5
33	59621.5
34	64381.5
35	68590.5
36	72661.0
37	76590.5
38	79778.0
39	81581.0
40	82540.5
41	82734.5
42	82159.5
43	80678.0
44	79056.5
45	77464.5
46	75991.5
47	74625.0
48	73315.0
49	73349.0
50	74514.0
51	71755.5
52	64854.5
53	59314.0
54	55012.0
55	51195.5
56	47923.0
57	44261.0
58	40295.0
59	36578.0
60	32326.0
61	27811.5
62	23756.5
63	19957.0
64	16356.5
65	13224.5
66	10745.0
67	8596.5
68	6750.5
69	5307.0
70	4164.5
71	3205.5
72	2525.5
73	1940.0
74	1425.5
75	1066.0
76	767.0
77	517.5
78	350.5
79	238.0
80	158.0
81	112.0
82	87.5
83	68.0
84	39.0
85	22.5
86	16.5
87	11.5
88	9.5
89	6.0
90	3.0
91	3.0
92	3.5
93	2.0
94	1.0
95	0.5
96	0.0
97	0.5
98	1.0
99	0.5
100	2.0
>>END_MODULE
>>Per base N content	fail
#Base	N-Count
1	8.154223122741396
2	24.691443596322614
3	6.225175727075028
4	24.151833473815977
5	12.447710958099865
6	10.072832307459361
7	7.964808788891103
8	8.659011703586165
9	5.143273728260776
10-11	6.91467525818069
12-13	9.946748621062824
14-15	7.434852980067968
16-17	8.093986806596455
18-19	6.711253917939159
20-21	8.156203494779039
22-23	5.823634667567736
24-25	5.4217016594279945
26-27	9.933422367559526
28-29	6.480169255796817
30-31	4.366204621115634
32-33	8.914335294314228
34-35	7.371419188237251
36-37	7.006164320544668
38-39	6.275015090022349
40-41	8.866435045653764
42-43	6.614999585359604
44-45	6.769097284538617
46-47	6.090840490521651
48-49	4.044125989618725
50-51	6.6727398075821025
52-53	4.675637751997185
54-55	5.164954676297876
56-57	4.8427729004240065
58-59	3.827976633260266
60-61	4.337241680065121
62-63	3.1940512924609297
64-65	4.619032117921253
66-67	2.6500472194957725
68-69	5.238249070566019
70-71	2.668943269354939
72-73	1.6042251237423093
74-75	0.8614618363742358
76-77	1.1175280666164147
78-79	1.8354541880123956
80-81	2.2254637061755016
82-83	0.8082805956133934
84-85	1.2726159518142477
86-87	0.4372909005618068
88-89	0.642506952962451
90-91	0.23677823175056223
92-93	0.32630755095229075
94-95	0.572657580884789
96-97	0.5854268547525009
98-99	0.4705859054446616
100	0.4071314847385517
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	2423787.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	92.64471520961335
#Duplication Level	Percentage of deduplicated	Percentage of total
1	95.81549908647223	88.76799625533187
2	3.096237996783389	5.737001748663617
3	0.575226694562725	1.598751398961927
4	0.20454381415751494	0.757996136420442
5	0.09663451489861921	0.4476338556100858
6	0.05638571611956394	0.3134303167072264
7	0.036741510000588715	0.23827347112629912
8	0.02372249664429345	0.17582111565372602
9	0.014671140211346207	0.12232832460124282
>10	0.07726792098180138	1.1489124453544455
>50	0.0014958666687553807	0.09192888646498289
>100	0.001168978523331679	0.22701979580897788
>500	3.144275368509142E-4	0.19404774472749414
>1k	8.983643909480527E-5	0.17885850456760233
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	4.12577507842067E-5	0.0
5	0.0	4.12577507842067E-5	0.0	8.25155015684134E-5	0.0
6	0.0	4.12577507842067E-5	0.0	8.25155015684134E-5	0.0
7	0.0	4.12577507842067E-5	0.0	1.650310031368268E-4	0.0
8	0.0	4.12577507842067E-5	0.0	2.062887539210335E-4	0.0
9	0.0	4.12577507842067E-5	0.0	2.062887539210335E-4	0.0
10-11	0.0	4.12577507842067E-5	0.0	2.062887539210335E-4	0.0
12-13	0.0	4.12577507842067E-5	0.0	2.2691762931313685E-4	0.0
14-15	0.0	8.25155015684134E-5	0.0	3.300620062736536E-4	0.0
16-17	0.0	8.25155015684134E-5	0.0	3.713197570578603E-4	0.0
18-19	4.12577507842067E-5	8.25155015684134E-5	0.0	4.12577507842067E-4	0.0
20-21	4.12577507842067E-5	8.25155015684134E-5	0.0	4.950930094104804E-4	0.0
22-23	4.12577507842067E-5	8.25155015684134E-5	0.0	5.36350760194687E-4	0.0
24-25	4.12577507842067E-5	8.25155015684134E-5	0.0	5.776085109788938E-4	0.0
26-27	4.12577507842067E-5	8.25155015684134E-5	0.0	5.982373863709972E-4	0.0
28-29	4.12577507842067E-5	8.25155015684134E-5	0.0	8.457838910762373E-4	0.0
30-31	8.25155015684134E-5	8.25155015684134E-5	0.0	0.0027642693025418485	0.0
32-33	8.25155015684134E-5	8.25155015684134E-5	0.0	0.007529539518117722	0.0
34-35	8.25155015684134E-5	8.25155015684134E-5	0.0	0.014007006391238174	0.0
36-37	8.25155015684134E-5	1.237732523526201E-4	0.0	0.02494031034905295	0.0
38-39	8.25155015684134E-5	1.650310031368268E-4	0.0	0.0418972459213619	0.0
40-41	8.25155015684134E-5	1.650310031368268E-4	0.0	0.060421976023470714	0.0
42-43	8.25155015684134E-5	1.650310031368268E-4	0.0	0.07851349974234535	0.0
44-45	8.25155015684134E-5	1.650310031368268E-4	0.0	0.10246362407257734	0.0
46-47	8.25155015684134E-5	1.8565987852893012E-4	0.0	0.13070455448436682	0.0
48-49	8.25155015684134E-5	2.062887539210335E-4	0.0	0.16036887729821142	0.0
50-51	8.25155015684134E-5	2.062887539210335E-4	0.0	0.1915597368910717	0.0
52-53	8.25155015684134E-5	2.062887539210335E-4	0.0	0.22625750530058952	0.0
54-55	8.25155015684134E-5	2.062887539210335E-4	0.0	0.26516356429009647	0.0
56-57	8.25155015684134E-5	2.062887539210335E-4	0.0	0.3124655755641894	0.0
58-59	8.25155015684134E-5	2.062887539210335E-4	0.0	0.36414090842140834	0.0
60-61	8.25155015684134E-5	2.062887539210335E-4	0.0	0.420168933986361	0.0
62-63	8.25155015684134E-5	2.062887539210335E-4	0.0	0.4770014856916057	0.0
64-65	8.25155015684134E-5	2.2691762931313685E-4	0.0	0.5453655787410363	0.0
66-67	8.25155015684134E-5	2.475465047052402E-4	0.0	0.6271178119199418	0.0
68-69	8.25155015684134E-5	2.475465047052402E-4	0.0	0.7187306475362728	0.0
70-71	8.25155015684134E-5	2.888042554894469E-4	0.0	0.8272591609741284	0.0
72-73	8.25155015684134E-5	2.888042554894469E-4	0.0	0.9392945832286418	0.0
74-75	8.25155015684134E-5	3.0943313088155024E-4	0.0	1.0570442039667678	0.0
76-77	8.25155015684134E-5	3.300620062736536E-4	0.0	1.1928440906729842	0.0
78-79	8.25155015684134E-5	3.300620062736536E-4	0.0	1.3454565108237646	0.0
80-81	8.25155015684134E-5	3.300620062736536E-4	0.0	1.5174394449677302	0.0
82-83	8.25155015684134E-5	3.300620062736536E-4	0.0	1.7079677380891969	0.0
84-85	8.25155015684134E-5	3.300620062736536E-4	0.0	1.8977327628211555	0.0
86-87	8.25155015684134E-5	3.300620062736536E-4	0.0	2.105568682396597	0.0
88	8.25155015684134E-5	3.713197570578603E-4	0.0	2.2829976396440776	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GACCGTC	240	8.6351065E-8	20.739634	7
CATGGGG	3775	0.0	18.74444	4
CCTATAC	590	4.0017767E-11	15.228703	3
GTGTTAG	940	0.0	15.174735	1
TATACCG	200	0.0037204672	15.020994	5
TAGTACG	305	1.1388405E-4	14.730213	4
GTACATG	10360	0.0	14.726396	1
GTATTAG	805	0.0	14.637902	1
CTATACG	270	7.569557E-4	14.559725	4
TACATGG	10570	0.0	14.253023	2
ACCGTCC	285	1.6733079E-4	14.0786085	8
GTATAAC	620	4.8803486E-9	14.004172	1
TATACTG	1045	0.0	13.895018	5
GTATAAT	1210	0.0	13.838834	1
GTCTATA	685	1.3569661E-9	13.580689	1
GTCTAGG	640	7.625204E-9	13.566543	1
GTCTAGA	690	1.5133992E-9	13.482279	1
GTATCGG	280	0.008378965	13.289675	1
TATCGTT	225	0.008448154	13.273366	7
ACATGGG	10490	0.0	13.008455	3
>>END_MODULE