FastQCFastQC Report
Wed 25 May 2016
SRR1295097_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295097_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2423787
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT62440.2576133958965866No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA47720.19688198674223437No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACCCGT1902.0381705E-417.3000286
GTATAGG8200.016.9980811
GAGTACT35350.016.33669712-13
CATGGGG46850.016.1601984
GTATTAG10400.015.7956081
GTATCAA111950.015.5632291
GTCTAAG6400.015.556281
GTACGGG1950.003267528315.3169531
CTAATAC12250.014.5864943
AGTACTT41100.014.45097812-13
TATCAAC121550.014.1612422
TAGACTG10000.014.1075515
ATCAACG119900.013.8831343
TCAACGC120000.013.8332384
CAACGCA120450.013.8205985
AACGCAG121800.013.6476646
GTACTTT42550.013.51659114-15
GTCCGTC2100.00787759313.4146869
TACTAGG7051.8189894E-1213.4074052
GCGTGTA2550.001900204112.888629
Produced by FastQC (version 0.11.5)