FastQCFastQC Report
Wed 25 May 2016
SRR1295096_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295096_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2896425
Sequences flagged as poor quality0
Sequence length100
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATAACG1650.006391643518.8107342
GTATAGG6650.018.6740781
GTATAGA9850.017.6503221
CATGGGG45150.016.9763574
GTCTAGG7301.8189894E-1215.3101871
GTATAAG9450.015.1121711
GTACTAG5801.875378E-914.9875581
TCTATAC8900.014.5646883
GTACATG107900.014.5589461
TACATGG111000.014.5401892
CTATACC9000.014.4028584
GTCTTAC9100.014.3287661
TACACTG16600.014.2157815
ACATGGG113800.014.1640393
GTCTAGT7653.45608E-1113.798071
CTACACT10650.013.7590074
TATACCG2200.0068758513.6933445
TATACTG13500.013.3890485
TTATACT16200.013.2200154
GTATATA15500.013.219441
Produced by FastQC (version 0.11.5)