##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1295096_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2896425 Sequences flagged as poor quality 0 Sequence length 100 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 26.33341930138015 33.0 27.0 33.0 6.0 33.0 2 22.45794349931381 27.0 2.0 33.0 2.0 33.0 3 24.812063146810292 27.0 15.0 33.0 15.0 33.0 4 24.756125913842062 33.0 6.0 37.0 2.0 37.0 5 26.71567363214998 33.0 15.0 37.0 2.0 37.0 6 27.799770061368758 33.0 15.0 37.0 2.0 37.0 7 27.642084293568796 33.0 15.0 37.0 6.0 37.0 8 28.351160482318722 33.0 22.0 37.0 6.0 37.0 9 29.41016045642473 37.0 27.0 37.0 15.0 37.0 10-11 28.974930129383637 35.0 24.5 37.0 10.5 37.0 12-13 27.769763415244654 33.0 15.0 37.0 4.0 37.0 14-15 29.545215222213592 33.0 27.0 37.0 6.0 40.0 16-17 29.972021543799684 37.0 27.0 40.0 6.0 40.0 18-19 30.15925891400606 37.0 27.0 40.0 10.5 40.0 20-21 29.656222066858284 35.0 27.0 40.0 6.0 40.0 22-23 30.431059150504502 37.0 27.0 40.0 15.0 40.0 24-25 30.58693924406812 37.0 27.0 40.0 15.0 40.0 26-27 29.150256091561147 33.0 22.0 40.0 4.0 40.0 28-29 29.86611477942636 33.0 27.0 40.0 10.5 40.0 30-31 30.575355136072915 37.0 27.0 40.0 15.0 40.0 32-33 29.35667607481637 33.0 27.0 40.0 6.0 40.0 34-35 28.954964309450443 33.0 22.0 37.0 6.0 40.0 36-37 29.267136038392156 33.0 22.0 37.0 6.0 40.0 38-39 29.371090568545707 33.0 24.5 37.0 6.0 40.0 40-41 28.402699534771315 33.0 22.0 37.0 6.0 40.0 42-43 29.062432136167864 33.0 22.0 37.0 6.0 40.0 44-45 28.945823213098905 33.0 22.0 37.0 6.0 40.0 46-47 28.660799261158154 33.0 22.0 37.0 6.0 40.0 48-49 28.609757200687053 33.0 22.0 37.0 6.0 40.0 50-51 28.593973087513056 33.0 22.0 37.0 6.0 40.0 52-53 28.731673010694216 33.0 22.0 37.0 6.0 40.0 54-55 28.695884754481817 33.0 22.0 37.0 6.0 40.0 56-57 28.68900334032471 33.0 22.0 37.0 6.0 40.0 58-59 28.6078926607801 33.0 22.0 37.0 10.5 40.0 60-61 28.539073167784423 33.0 22.0 37.0 10.5 40.0 62-63 28.489200134648748 33.0 22.0 37.0 10.5 40.0 64-65 28.043186514410007 33.0 22.0 37.0 6.0 40.0 66-67 28.210037546285506 33.0 22.0 37.0 10.5 40.0 68-69 27.76994053013629 33.0 22.0 37.0 6.0 38.5 70-71 27.902844368488743 33.0 22.0 37.0 10.5 37.0 72-73 27.95014526528393 33.0 22.0 37.0 10.5 37.0 74-75 28.0273659770234 33.0 22.0 37.0 15.0 37.0 76-77 27.82067514263273 33.0 22.0 37.0 15.0 37.0 78-79 27.379405646616085 33.0 22.0 37.0 10.5 37.0 80-81 27.154066305877073 27.0 22.0 33.0 6.0 37.0 82-83 27.279803896182365 27.0 22.0 33.0 15.0 37.0 84-85 27.237456001795316 27.0 22.0 33.0 15.0 37.0 86-87 27.015189759790086 27.0 22.0 33.0 15.0 37.0 88-89 26.999692897278543 27.0 22.0 33.0 15.0 37.0 90-91 27.159073167784424 27.0 22.0 33.0 15.0 37.0 92-93 27.077910872886402 27.0 22.0 33.0 15.0 37.0 94-95 26.95742406587431 27.0 22.0 33.0 15.0 37.0 96-97 27.03942808807409 27.0 22.0 33.0 15.0 37.0 98-99 27.120985870512786 27.0 22.0 33.0 15.0 37.0 100 26.80111551308941 27.0 22.0 33.0 15.0 37.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 7.347091102310145 1101 2 11.293207003955626 1101 3 8.491706187211193 1101 4 12.842235735632723 1101 5 10.833586779898354 1101 6 10.076467739817954 1101 7 9.687179656495655 1101 8 9.230762286492727 1101 9 7.426237511581856 1101 10-11 7.929504561648667 1101 12-13 9.305949950158396 1101 14-15 8.243461775708859 1101 16-17 8.36825605010603 1101 18-19 8.362963964495986 1101 20-21 7.475531699654564 1101 22-23 7.5900617113739415 1101 24-25 7.507361518866798 1101 26-27 8.793860819152307 1101 28-29 8.494047681135978 1101 30-31 5.801110176315948 1101 32-33 7.390807623502692 1101 34-35 8.93921062640143 1101 36-37 8.433105670104158 1101 38-39 8.399062720717083 1101 40-41 9.283923649957917 1101 42-43 8.79979200982175 1101 44-45 8.585929002327312 1101 46-47 7.498959879437173 1101 48-49 5.668028134150827 1101 50-51 6.985970790646697 1101 52-53 6.143190167648317 1101 54-55 4.215803875908648 1101 56-57 6.378720395306029 1101 58-59 6.414480568043544 1101 60-61 6.99110236727428 1101 62-63 6.49385710585053 1101 64-65 6.917240606429832 1101 66-67 6.015915741153815 1101 68-69 6.987929664645257 1101 70-71 6.473695830160903 1101 72-73 5.678011373808236 1101 74-75 5.152929356918616 1101 76-77 5.312334347553897 1101 78-79 5.36352299244011 1101 80-81 5.182121441404245 1101 82-83 4.746100824960102 1101 84-85 4.573030695593989 1101 86-87 4.43015201833942 1101 88-89 4.304888437410323 1101 90-91 4.109967832147699 1101 92-93 4.012439292139074 1101 94-95 3.8811379631581495 1101 96-97 4.114340337736188 1101 98-99 3.8858470142800883 1101 100 4.043004266299366 1102 1 6.7273339695135554 1102 2 10.530176873956961 1102 3 8.191565720129596 1102 4 12.744758813727092 1102 5 10.63760436683377 1102 6 9.668518216606625 1102 7 6.830609870241634 1102 8 7.895510378386369 1102 9 6.625372249586011 1102 10-11 7.653434213906994 1102 12-13 9.29375234457017 1102 14-15 6.564476640856654 1102 16-17 7.6438842501098385 1102 18-19 7.679464088213603 1102 20-21 7.3376664947591586 1102 22-23 6.960109485408459 1102 24-25 7.260552481770549 1102 26-27 8.712016474950858 1102 28-29 7.928644647199288 1102 30-31 4.550879871209265 1102 32-33 4.775110902971271 1102 34-35 5.282519977550102 1102 36-37 5.472241550117097 1102 38-39 4.7454450271941795 1102 40-41 6.382382889760731 1102 42-43 7.254299899199161 1102 44-45 6.589391382654306 1102 46-47 5.532749064613899 1102 48-49 3.831272390798265 1102 50-51 5.407630510093199 1102 52-53 6.172866408155368 1102 54-55 5.498727799089522 1102 56-57 6.51661357941688 1102 58-59 6.221659044984484 1102 60-61 6.1865971425840485 1102 62-63 4.4005671688513495 1102 64-65 6.3111038318433295 1102 66-67 4.031952209301284 1102 68-69 6.328450801324681 1102 70-71 5.511010777152656 1102 72-73 5.062913636888997 1102 74-75 4.296028578449288 1102 76-77 3.6208284186249067 1102 78-79 4.5825187860410495 1102 80-81 4.540600476026146 1102 82-83 4.055964164574483 1102 84-85 3.166274190902236 1102 86-87 3.404426366918571 1102 88-89 3.500265776149167 1102 90-91 3.40024534028451 1102 92-93 3.3519248171776184 1102 94-95 3.121540140588248 1102 96-97 3.372807381265755 1102 98-99 3.1887724601393543 1102 100 3.3075121556767826 1103 1 6.8214337796066395 1103 2 11.02677715103426 1103 3 8.075804458026923 1103 4 12.072290568653774 1103 5 10.465787019926765 1103 6 9.258069133452306 1103 7 8.37379945026716 1103 8 8.260559333898335 1103 9 7.288900081845281 1103 10-11 7.671052438744322 1103 12-13 8.946325622176719 1103 14-15 8.785961806324721 1103 16-17 8.34042813884307 1103 18-19 7.594333060225676 1103 20-21 8.6370963848622 1103 22-23 7.113869759061217 1103 24-25 5.523609360448052 1103 26-27 6.205305872852541 1103 28-29 7.257928916057661 1103 30-31 6.4408044921737435 1103 32-33 8.292040754158972 1103 34-35 7.499175709003044 1103 36-37 4.550635474652449 1103 38-39 6.795696965178671 1103 40-41 7.2519275412348385 1103 42-43 7.785579358120124 1103 44-45 6.018743013985834 1103 46-47 6.764124761198687 1103 48-49 6.004210733315627 1103 50-51 6.846893920323261 1103 52-53 6.523996237550467 1103 54-55 5.2204239635924985 1103 56-57 6.231275366098497 1103 58-59 5.859354400048147 1103 60-61 5.387311433857668 1103 62-63 3.1076879776680144 1103 64-65 5.334083697040558 1103 66-67 4.484979706888534 1103 68-69 5.498497344084868 1103 70-71 4.0598537070116265 1103 72-73 4.640062698250723 1103 74-75 3.6620746230929804 1103 76-77 4.035806003382543 1103 78-79 3.2906914378887535 1103 80-81 4.488205197236571 1103 82-83 3.8935162333145605 1103 84-85 3.6692436657006553 1103 86-87 3.2273002266987767 1103 88-89 3.333747338055705 1103 90-91 3.030221913243011 1103 92-93 2.6041736964155007 1103 94-95 3.168269577716604 1103 96-97 3.2572945547659558 1103 98-99 3.0670794866951887 1103 100 3.2692308910835273 1104 1 6.416903812306558 1104 2 9.771988881080834 1104 3 7.417981155716586 1104 4 12.311981805991127 1104 5 9.53284566634845 1104 6 8.327847056054882 1104 7 4.226510246574893 1104 8 6.782428355111357 1104 9 6.79662962915344 1104 10-11 7.24882430919472 1104 12-13 7.6101327136373875 1104 14-15 7.549755415739867 1104 16-17 7.356094704513485 1104 18-19 7.01074318690927 1104 20-21 7.67339643516976 1104 22-23 6.673540673286823 1104 24-25 6.199287559742221 1104 26-27 8.429595897853872 1104 28-29 7.351561960503634 1104 30-31 6.468521529287006 1104 32-33 7.904673716664593 1104 34-35 8.165476740689183 1104 36-37 6.802982922687018 1104 38-39 7.5654974690165275 1104 40-41 7.422780073382132 1104 42-43 7.485371363028925 1104 44-45 6.914081608651706 1104 46-47 6.9771013014866625 1104 48-49 5.7682334898651355 1104 50-51 5.8761085125652315 1104 52-53 6.065055735770645 1104 54-55 4.937510145782095 1104 56-57 6.306155266321834 1104 58-59 4.00259517890068 1104 60-61 4.299842256767427 1104 62-63 4.152610820350169 1104 64-65 3.072984352944456 1104 66-67 3.521009401689323 1104 68-69 4.35657952072539 1104 70-71 2.8286764052501 1104 72-73 2.6427009868789284 1104 74-75 3.3986602259833774 1104 76-77 3.9900023019655464 1104 78-79 4.38896387811041 1104 80-81 3.0476593506593908 1104 82-83 3.108592495005354 1104 84-85 3.212233287860375 1104 86-87 1.0599511208013581 1104 88-89 0.5602778598823299 1104 90-91 2.3803325327120355 1104 92-93 2.649441688991324 1104 94-95 2.682478367438616 1104 96-97 3.066872333305973 1104 98-99 2.666939997545615 1104 100 0.9002158712272852 1105 1 5.916066326542381 1105 2 -2.816433940762849 1105 3 0.5304982046380857 1105 4 8.503983669792671 1105 5 8.687187926765134 1105 6 8.490876902279584 1105 7 5.610943123656522 1105 8 6.384079246968607 1105 9 5.309094265947849 1105 10-11 5.390335912271386 1105 12-13 5.447607434887754 1105 14-15 6.137482334558499 1105 16-17 6.291567960196904 1105 18-19 6.343459919459637 1105 20-21 7.397003145682536 1105 22-23 2.399538620814347 1105 24-25 4.4548787920269355 1105 26-27 7.25474374708746 1105 28-29 6.252821574939372 1105 30-31 5.625956882051536 1105 32-33 7.425723994392126 1105 34-35 6.517602173345093 1105 36-37 6.559938294057755 1105 38-39 4.445635934814302 1105 40-41 5.770420528099233 1105 42-43 5.949345449463642 1105 44-45 6.478361948626329 1105 46-47 5.81275840792469 1105 48-49 5.412561117944353 1105 50-51 6.266104119788409 1105 52-53 5.929825024045879 1105 54-55 5.525259137190677 1105 56-57 5.308758492997757 1105 58-59 2.005455547935295 1105 60-61 4.69305734677242 1105 62-63 -0.816794197013337 1105 64-65 -0.24208792239250343 1105 66-67 0.9754934410459306 1105 68-69 3.1039301073207106 1105 70-71 3.1366237678632736 1105 72-73 2.8368352219511372 1105 74-75 2.9610357743579847 1105 76-77 2.00346147796143 1105 78-79 3.3729529251212362 1105 80-81 1.7340035788110129 1105 82-83 1.1092451675704496 1105 84-85 1.4824068529206365 1105 86-87 -0.1696749241866371 1105 88-89 1.7917327084309527 1105 90-91 1.565820476941802 1105 92-93 1.9149243448141284 1105 94-95 1.297503830325354 1105 96-97 2.1409127748510954 1105 98-99 2.017570844097449 1105 100 2.1478211476712943 1106 1 6.238894451441265 1106 2 10.482915187209972 1106 3 6.874091021334415 1106 4 11.934558010514234 1106 5 10.114357459203248 1106 6 9.522183758775302 1106 7 6.890780793936415 1106 8 7.6486663623221105 1106 9 6.9968189965739604 1106 10-11 5.87831517776241 1106 12-13 8.015307043365343 1106 14-15 8.067599532422918 1106 16-17 7.9520394308327305 1106 18-19 7.412784248856166 1106 20-21 8.393837418453938 1106 22-23 6.405328441231752 1106 24-25 5.227089670525569 1106 26-27 7.896442177762104 1106 28-29 6.489928502620966 1106 30-31 5.511571598116898 1106 32-33 7.50410367405561 1106 34-35 6.945352427349295 1106 36-37 6.106038497908262 1106 38-39 7.225925027194172 1106 40-41 6.822499194981614 1106 42-43 6.324294758636917 1106 44-45 4.659150740084023 1106 46-47 6.082101996340803 1106 48-49 5.995690061480996 1106 50-51 6.679664566318095 1106 52-53 5.750864480444523 1106 54-55 5.3339541444710505 1106 56-57 5.082889242067484 1106 58-59 5.188038482735486 1106 60-61 5.095561640576015 1106 62-63 5.0633681327067706 1106 64-65 5.310023671200764 1106 66-67 3.2279441771727697 1106 68-69 3.490854656746368 1106 70-71 3.0677458775542625 1106 72-73 2.90876006259181 1106 74-75 2.9309040804573208 1106 76-77 3.69821251501045 1106 78-79 4.237286657526994 1106 80-81 3.8404491507249396 1106 82-83 2.231132357345569 1106 84-85 2.765667283271714 1106 86-87 3.4343518287659585 1106 88-89 3.4596624026551908 1106 90-91 3.2700908021319 1106 92-93 3.064639355330229 1106 94-95 1.6366736345641506 1106 96-97 1.685485614197482 1106 98-99 2.070501295480721 1106 100 3.0478803484478654 1107 1 6.059107931176452 1107 2 10.311745337379516 1107 3 6.075782996144685 1107 4 11.414755007123315 1107 5 9.503623301283014 1107 6 8.169831903454774 1107 7 3.8065492551022473 1107 8 6.332848038408013 1107 9 5.702786430773866 1107 10-11 4.75021966347208 1107 12-13 6.319717532627418 1107 14-15 6.451496965008097 1107 16-17 7.1920642201109395 1107 18-19 6.424414631546309 1107 20-21 5.65359619126194 1107 22-23 5.877566025601205 1107 24-25 4.799277250050839 1107 26-27 7.374447344886278 1107 28-29 6.599290401539104 1107 30-31 5.237856487508143 1107 32-33 5.040997844893653 1107 34-35 6.715660685059614 1107 36-37 6.550246461738393 1107 38-39 6.581895858778218 1107 40-41 7.865232810353806 1107 42-43 6.358708283584441 1107 44-45 4.221098978417629 1107 46-47 3.613410544701857 1107 48-49 4.296035612910028 1107 50-51 5.217097254837391 1107 52-53 3.9472464213455396 1107 54-55 5.458299773406946 1107 56-57 5.38317820056902 1107 58-59 3.1705407659584814 1107 60-61 2.9494590860790026 1107 62-63 0.5483941168824558 1107 64-65 2.206091928911082 1107 66-67 4.304499320076051 1107 68-69 5.257049082711166 1107 70-71 4.397837710268618 1107 72-73 2.508737727697575 1107 74-75 2.6811595301585953 1107 76-77 3.187479028816359 1107 78-79 3.9924521023840924 1107 80-81 2.247203189155428 1107 82-83 1.8036945792089618 1107 84-85 2.575829375957003 1107 86-87 2.499869012683284 1107 88-89 2.736110599005908 1107 90-91 1.552981704763905 1107 92-93 2.427019875272098 1107 94-95 2.4011674695391214 1107 96-97 2.463544364399322 1107 98-99 1.895639780867448 1107 100 0.16267999966698454 1108 1 4.469200736790363 1108 2 -3.2110987830728206 1108 3 0.09515176586229401 1108 4 6.706754847069597 1108 5 7.116601216992034 1108 6 7.040724177372521 1108 7 4.626764663564149 1108 8 5.243116363919807 1108 9 5.04529513653242 1108 10-11 3.601062939385674 1108 12-13 2.764328305547803 1108 14-15 4.173111339420842 1108 16-17 4.868421562163611 1108 18-19 4.276083574627062 1108 20-21 4.966027710832908 1108 22-23 4.476748105714904 1108 24-25 3.262729523537228 1108 26-27 5.012709632627097 1108 28-29 6.331177443346331 1108 30-31 4.779449878992441 1108 32-33 4.8006706655878055 1108 34-35 3.149891094868071 1108 36-37 1.4160431248454728 1108 38-39 2.7288800807171043 1108 40-41 5.6394285191548015 1108 42-43 3.46227821925363 1108 44-45 4.022859115223824 1108 46-47 4.938593435867887 1108 48-49 5.039070793227285 1108 50-51 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-23.826985241363083 2213 44-45 -17.73350325006376 2213 46-47 -17.53594739610583 2213 48-49 -6.55661699927434 2213 50-51 -23.983855433681903 2213 52-53 -18.311630297880598 2213 54-55 -18.220802440093813 2213 56-57 -18.098304912283908 2213 58-59 -18.5917655895141 2213 60-61 -19.98761497682957 2213 62-63 -18.074971834452505 2213 64-65 -18.146727790211386 2213 66-67 -8.972461405750053 2213 68-69 -23.290585343253632 2213 70-71 -17.880953038701893 2213 72-73 -9.776169592580604 2213 74-75 -4.745670632186357 2213 76-77 -3.450561278092998 2213 78-79 -4.980964902407322 2213 80-81 -3.6729040840862233 2213 82-83 -3.6985475769729845 2213 84-85 -3.373572585365398 2213 86-87 -3.5187812418415945 2213 88-89 -3.2109142147503924 2213 90-91 -3.1011067676546347 2213 92-93 -3.9248757842428432 2213 94-95 -3.8719336560762407 2213 96-97 -3.6866081132245228 2213 98-99 -4.179338901563614 2213 100 -3.7630012279068126 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 807.0 3 739.0 4 4255.0 5 15805.0 6 21725.0 7 25918.0 8 24432.0 9 24319.0 10 26761.0 11 31016.0 12 38941.0 13 53133.0 14 39993.0 15 44367.0 16 51060.0 17 55310.0 18 56881.0 19 57199.0 20 56262.0 21 55456.0 22 57316.0 23 63036.0 24 69381.0 25 76882.0 26 84895.0 27 91718.0 28 99501.0 29 104831.0 30 110164.0 31 120951.0 32 138253.0 33 169428.0 34 206641.0 35 243703.0 36 269520.0 37 234334.0 38 71435.0 39 57.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.00278012029352 20.84362972626102 13.924679766165433 24.228910387280024 2 15.263326824832161 21.145788274183445 40.08995381946334 23.500931081521053 3 18.19646159952152 26.29924318220931 30.051971569859802 25.452323648409365 4 12.440742118063904 18.219641094780894 38.864666558489766 30.47495022866544 5 13.222937858478309 38.06001837944038 35.840186012612094 12.876857749469215 6 30.26626369795068 38.092332229599705 18.78250283638353 12.858901236066087 7 26.08124823476008 32.2439334979825 23.58131122867468 18.093507038582736 8 24.11254611002199 36.16851487121905 21.62056995752819 18.098369061230763 9 24.771363141891197 17.179395266872504 21.800146902296778 36.24909468893952 10-11 23.102774564862944 27.269202004858855 29.50713731610901 20.12088611416919 12-13 24.17839782001201 24.831181997224636 29.025674543328073 21.964745639435282 14-15 21.602496254605065 26.611664170987364 26.326608943999357 25.459230630408218 16-17 21.269097132188975 29.810714100503034 27.193825932403954 21.726362834904037 18-19 21.410637251384937 29.088655379536743 28.539334299975287 20.961373069103033 20-21 22.398588665973527 28.4414520319433 27.87073411895481 21.289225183128362 22-23 21.92739274412966 28.28412308930452 27.884080274865752 21.904403891700067 24-25 22.004857001011402 28.471483006626723 27.602723595066436 21.92093639729544 26-27 21.864884441371643 28.080853012964962 27.827749128303097 22.226513417360298 28-29 21.909308263922746 28.066701412759375 28.101619942656015 21.92237038066186 30-31 22.235187149019833 28.45196603165058 27.663740693745392 21.64910612558419 32-33 21.652392447904653 28.215894450319663 27.971716943921937 22.159996157853744 34-35 21.616733302017305 27.9979131785838 27.983333093447314 22.40202042595158 36-37 22.2349553854569 28.217323589760223 27.57302180148719 21.974699223295683 38-39 22.066204803051537 28.06020976357169 27.768549208574246 22.105036224802525 40-41 21.924613887135656 28.14099298114872 27.688094568184756 22.24629856353087 42-43 22.08706786171575 28.0166275533214 27.808551715149193 22.087752869813656 44-45 22.264464685693426 27.92104444202953 27.831534274618676 21.982956597658372 46-47 22.015732535859307 28.068489811136764 28.0347346858181 21.881042967185827 48-49 22.045686567889764 27.8990912741068 27.957968501583565 22.09725365641987 50-51 22.022571923804975 27.971996448380505 27.920728532784306 22.084703095030207 52-53 21.762105390400123 27.896136028972023 27.723673136535904 22.61808544409195 54-55 22.010235173455055 28.236820737528983 27.6229910755792 22.129953013436765 56-57 22.21405058040177 27.873233829651454 27.552939417763245 22.359776172183526 58-59 21.974876962368388 27.504102446872103 27.99315847447289 22.527862116286617 60-61 22.133122046819466 27.964398967904096 27.758012252261093 22.144466733015346 62-63 21.880977795061693 27.995324788370574 27.792985364655635 22.3307120519121 64-65 21.80072588492637 27.862090899152232 27.87614961144285 22.46103360447855 66-67 21.983788205917143 27.778634333682206 27.640528740033076 22.597048720367578 68-69 22.490546128035565 27.720843324785566 27.507501798826272 22.281108748352597 70-71 22.263791333709975 27.814971660409114 27.599014623914027 22.322222381966885 72-73 22.28507474584586 27.845018038104598 27.6153671376743 22.25454007837524 74-75 22.345624244534527 27.80344962391797 27.472226694855014 22.378699436692497 76-77 22.308710692719036 27.871398107770496 27.57163880584556 22.248252393664906 78-79 22.401394348021274 27.772002003988273 27.70728401889716 22.119319629093294 80-81 22.267014830342763 27.688408846462437 27.70550322050221 22.339073102692595 82-83 22.031772502415585 27.805591338801346 27.720588465881946 22.442047692901117 84-85 22.182240140478132 27.795468964390206 27.372204408558165 22.6500864865735 86-87 22.30197023340404 27.676616073850667 27.559040485946678 22.462373206798613 88-89 22.24300622181141 27.44286037447018 27.803653451555228 22.510479952163184 90-91 22.337341241225037 27.505127115232618 27.649266361535858 22.508265282006487 92-93 22.1220081930283 27.69773825284711 27.661659816038096 22.518593738086494 94-95 21.975239504373203 27.555756972433354 27.74250376889463 22.726499754298814 96-97 22.197919114417445 27.490288453806528 27.700158931209756 22.61163350056627 98-99 22.299510974875663 27.466593113402133 27.606830045120766 22.62706586660144 100 22.163559269384773 27.428231495920336 27.64598473644882 22.76222449824607 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 412.0 1 477.5 2 466.0 3 391.0 4 414.5 5 535.0 6 801.0 7 1195.5 8 1706.0 9 2423.0 10 3312.0 11 4280.0 12 5270.0 13 6267.5 14 7252.0 15 8086.0 16 9007.0 17 9880.0 18 10651.5 19 11459.0 20 12314.0 21 13272.5 22 14545.5 23 16531.0 24 18943.5 25 21805.0 26 25872.0 27 31440.0 28 38325.5 29 46043.5 30 53738.5 31 61091.0 32 68199.0 33 74931.5 34 81408.0 35 87121.5 36 92131.5 37 96869.5 38 100052.5 39 101623.0 40 102305.5 41 101693.0 42 100374.5 43 98426.0 44 95827.5 45 93270.0 46 90930.0 47 88351.5 48 86000.0 49 85445.5 50 86137.0 51 83083.0 52 75621.5 53 68662.5 54 63233.0 55 58734.0 56 54318.5 57 49447.0 58 44915.5 59 40289.5 60 35014.5 61 29963.0 62 25176.5 63 20646.5 64 16882.0 65 13786.5 66 11006.5 67 8631.0 68 6679.5 69 5198.5 70 4060.5 71 3091.5 72 2392.5 73 1817.0 74 1376.0 75 1016.5 76 718.0 77 522.5 78 336.0 79 204.0 80 145.0 81 102.0 82 70.0 83 53.5 84 39.5 85 30.5 86 20.0 87 11.5 88 8.0 89 5.5 90 3.5 91 2.0 92 0.0 93 1.0 94 1.5 95 3.0 96 2.5 97 1.0 98 1.0 99 0.0 100 0.5 >>END_MODULE >>Per base N content fail #Base N-Count 1 8.3752557031513 2 25.133707933055405 3 6.312126155519304 4 24.891927117049466 5 12.838758124239364 6 10.381418472772468 7 8.197622931717548 8 9.231621740594008 9 5.80305031202258 10-11 7.0936585618477945 12-13 10.569426102868192 14-15 7.980596770156313 16-17 8.459134968107236 18-19 6.886403065848416 20-21 8.498977187394805 22-23 6.135701770285784 24-25 5.664931769336337 26-27 9.889070146818923 28-29 6.564143728907188 30-31 4.5766073694295555 32-33 9.062223257981822 34-35 7.648860232873283 36-37 7.404023926046764 38-39 6.5547701045254065 40-41 9.196336863547304 42-43 6.757537308923932 44-45 6.753653210423193 46-47 6.105388539320024 48-49 4.124394727983635 50-51 6.561675168526718 52-53 4.889803809869063 54-55 5.307439343328413 56-57 5.077966803904814 58-59 3.4919599161034722 60-61 4.288113795454741 62-63 3.1414761300568808 64-65 4.4695616147492165 66-67 2.7030390912935776 68-69 5.377284065701684 70-71 2.7423289054610422 72-73 1.6862856797603945 74-75 1.0436486358182933 76-77 1.1495032669584055 78-79 1.8675781351148397 80-81 2.4490190493453134 82-83 0.9156805372139794 84-85 1.338063302174232 86-87 0.6016382264343112 88-89 0.7186617985965457 90-91 0.29665881215636514 92-93 0.3813321594724531 94-95 0.6189871997376075 96-97 0.6044175146948393 98-99 0.4888785312928869 100 0.524163408339591 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 2896425.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 92.53826036445226 #Duplication Level Percentage of deduplicated Percentage of total 1 95.25756098714714 88.14968980311312 2 3.5833437199562606 6.631927882652748 3 0.6657143760369356 1.8481215077419448 4 0.23362134199053655 0.8647565028725206 5 0.09359796331966298 0.4330696349628713 6 0.05135006066545164 0.285110717015399 7 0.026459443133332296 0.17139575864395057 8 0.021286437037948348 0.15758478822793476 9 0.013785620390309382 0.11481275960675565 >10 0.05089426717483767 0.7413530430612286 >50 9.152475630021282E-4 0.05755508800603975 >100 0.0011324395978144128 0.21798817141158627 >500 2.629635453619455E-4 0.16571582721866152 >1k 7.513244141150821E-5 0.16091851546521488 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 1.0357596001967943E-4 0.0 5 0.0 0.0 0.0 1.0357596001967943E-4 0.0 6 0.0 0.0 0.0 1.0357596001967943E-4 0.0 7 3.452532000655981E-5 0.0 0.0 1.0357596001967943E-4 0.0 8 3.452532000655981E-5 0.0 0.0 1.0357596001967943E-4 0.0 9 3.452532000655981E-5 0.0 0.0 1.0357596001967943E-4 0.0 10-11 3.452532000655981E-5 0.0 0.0 1.0357596001967943E-4 0.0 12-13 3.452532000655981E-5 0.0 0.0 1.2083862002295934E-4 0.0 14-15 3.452532000655981E-5 0.0 0.0 1.5536394002951915E-4 0.0 16-17 3.452532000655981E-5 0.0 0.0 1.7262660003279905E-4 0.0 18-19 3.452532000655981E-5 0.0 0.0 2.0715192003935886E-4 0.0 20-21 3.452532000655981E-5 0.0 0.0 2.4167724004591867E-4 0.0 22-23 3.452532000655981E-5 0.0 0.0 3.62515860068878E-4 0.0 24-25 3.452532000655981E-5 0.0 0.0 4.8335448009183735E-4 0.0 26-27 3.452532000655981E-5 0.0 0.0 6.559810801246365E-4 0.0 28-29 3.452532000655981E-5 0.0 0.0 0.0012256488602328734 0.0 30-31 6.905064001311962E-5 3.452532000655981E-5 0.0 0.0028656015605444643 0.0 32-33 6.905064001311962E-5 3.452532000655981E-5 0.0 0.007612833061446438 0.0 34-35 6.905064001311962E-5 5.1787980009839716E-5 0.0 0.015484606022942077 0.0 36-37 6.905064001311962E-5 6.905064001311962E-5 0.0 0.026808910985093692 0.0 38-39 6.905064001311962E-5 8.631330001639953E-5 0.0 0.044589450788472 0.0 40-41 6.905064001311962E-5 1.0357596001967943E-4 0.0 0.06701364613273258 0.0 42-43 6.905064001311962E-5 1.0357596001967943E-4 0.0 0.0884020818767964 0.0 44-45 6.905064001311962E-5 1.0357596001967943E-4 0.0 0.1170926228022476 0.0 46-47 6.905064001311962E-5 1.0357596001967943E-4 0.0 0.15082386044865653 0.0 48-49 6.905064001311962E-5 1.0357596001967943E-4 0.0 0.18678198123548856 0.0 50-51 6.905064001311962E-5 1.3810128002623924E-4 0.0 0.22629620998299627 0.0 52-53 6.905064001311962E-5 1.5536394002951915E-4 0.0 0.26734681547079586 0.0 54-55 6.905064001311962E-5 1.7262660003279905E-4 0.0 0.3136797949195992 0.0 56-57 6.905064001311962E-5 1.7262660003279905E-4 0.0 0.3690066202301112 0.0 58-59 6.905064001311962E-5 1.7262660003279905E-4 0.0 0.4301164366417221 0.0 60-61 1.0357596001967943E-4 1.7262660003279905E-4 0.0 0.4927971551136314 0.0 62-63 1.0357596001967943E-4 1.7262660003279905E-4 0.0 0.5598798518863772 0.0 64-65 1.0357596001967943E-4 1.7262660003279905E-4 0.0 0.6350069482206513 0.0 66-67 1.2083862002295934E-4 1.7262660003279905E-4 0.0 0.7263782076180119 0.0 68-69 1.3810128002623924E-4 1.8988926003607896E-4 0.0 0.8288666200574847 0.0 70-71 1.3810128002623924E-4 2.0715192003935886E-4 0.0 0.9498778666804768 0.0 72-73 1.3810128002623924E-4 2.4167724004591867E-4 0.0 1.0740481800840693 0.0 74-75 1.3810128002623924E-4 2.4167724004591867E-4 0.0 1.2062801557091931 0.0 76-77 1.3810128002623924E-4 2.4167724004591867E-4 0.0 1.355205123557489 0.0 78-79 1.3810128002623924E-4 2.4167724004591867E-4 0.0 1.5212028621490286 0.0 80-81 1.3810128002623924E-4 2.589399000491986E-4 0.0 1.708088419344537 0.0 82-83 1.3810128002623924E-4 2.9346522005575836E-4 0.0 1.9162243110040826 0.0 84-85 1.3810128002623924E-4 3.107278800590383E-4 0.0 2.130022355144704 0.0 86-87 1.3810128002623924E-4 3.107278800590383E-4 0.0 2.362429546768862 0.0 88 1.3810128002623924E-4 3.107278800590383E-4 0.0 2.5620204217267837 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TATAACG 165 0.0063916435 18.810734 2 GTATAGG 665 0.0 18.674078 1 GTATAGA 985 0.0 17.650322 1 CATGGGG 4515 0.0 16.976357 4 GTCTAGG 730 1.8189894E-12 15.310187 1 GTATAAG 945 0.0 15.112171 1 GTACTAG 580 1.875378E-9 14.987558 1 TCTATAC 890 0.0 14.564688 3 GTACATG 10790 0.0 14.558946 1 TACATGG 11100 0.0 14.540189 2 CTATACC 900 0.0 14.402858 4 GTCTTAC 910 0.0 14.328766 1 TACACTG 1660 0.0 14.215781 5 ACATGGG 11380 0.0 14.164039 3 GTCTAGT 765 3.45608E-11 13.79807 1 CTACACT 1065 0.0 13.759007 4 TATACCG 220 0.00687585 13.693344 5 TATACTG 1350 0.0 13.389048 5 TTATACT 1620 0.0 13.220015 4 GTATATA 1550 0.0 13.21944 1 >>END_MODULE