FastQCFastQC Report
Wed 25 May 2016
SRR1295096_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295096_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2896425
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT68710.23722347376507247No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA50930.17583745479340912No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGTACT41100.018.56098712-13
GTATCAA117400.018.1509971
CATGGGG50650.017.5444564
TATACCG1700.001914871216.5943375
TCCGTAC2002.9866843E-416.4554823
TATCAAC130900.015.8082062
CAACGCA127950.015.727555
ATCAACG128550.015.6170263
TCAACGC128800.015.5872544
AACGCAG130450.015.4781466
TAGACAG13750.015.3874755
AGTACTT51500.014.9950612-13
GTACTTT52050.014.74629214-15
GTCTAAG8250.014.5177071
GGATACG2751.2891351E-414.5177061
GTACGAC1950.00477801914.4663563
ACGCAGA140500.014.3675167
GTCCTAT8050.014.2584621
CGCAGAG142350.014.1148358
GTACATG138750.014.0991791
Produced by FastQC (version 0.11.5)