##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1295096_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2896425 Sequences flagged as poor quality 0 Sequence length 100 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.326548417445643 33.0 33.0 33.0 27.0 33.0 2 29.92898866706371 33.0 33.0 33.0 27.0 33.0 3 30.808874733507686 33.0 33.0 33.0 27.0 33.0 4 35.132906945631255 37.0 37.0 37.0 33.0 37.0 5 35.43530144920031 37.0 37.0 37.0 33.0 37.0 6 35.15412068325608 37.0 37.0 37.0 33.0 37.0 7 35.057654867638554 37.0 37.0 37.0 33.0 37.0 8 35.04605332435675 37.0 37.0 37.0 33.0 37.0 9 35.03308078061749 37.0 37.0 37.0 33.0 37.0 10-11 35.00506331080556 37.0 37.0 37.0 33.0 37.0 12-13 34.90156296123669 37.0 37.0 37.0 33.0 37.0 14-15 36.32143331866007 37.0 37.0 40.0 30.0 40.0 16-17 36.30866758158765 37.0 37.0 40.0 27.0 40.0 18-19 36.33652537179454 38.5 37.0 40.0 30.0 40.0 20-21 36.339376645347286 40.0 37.0 40.0 27.0 40.0 22-23 36.310540062318196 38.5 37.0 40.0 27.0 40.0 24-25 36.26742570582701 37.0 37.0 40.0 27.0 40.0 26-27 36.24274994173852 37.0 37.0 40.0 27.0 40.0 28-29 36.183132309657594 37.0 37.0 40.0 27.0 40.0 30-31 36.08485460524612 37.0 37.0 40.0 27.0 40.0 32-33 36.03849400554131 37.0 37.0 40.0 27.0 40.0 34-35 35.907401020223205 37.0 37.0 40.0 27.0 40.0 36-37 35.912210397300115 37.0 37.0 40.0 27.0 40.0 38-39 35.84247753696367 37.0 37.0 40.0 27.0 40.0 40-41 35.772056932252696 37.0 37.0 40.0 27.0 40.0 42-43 35.66327679812183 37.0 37.0 40.0 27.0 40.0 44-45 35.53830515204088 37.0 37.0 40.0 27.0 40.0 46-47 35.42322362912901 37.0 37.0 40.0 27.0 40.0 48-49 35.319052625219015 37.0 33.0 40.0 27.0 40.0 50-51 35.219660616104335 37.0 33.0 40.0 27.0 40.0 52-53 35.121620618521106 37.0 33.0 40.0 27.0 40.0 54-55 34.98670878755708 37.0 33.0 40.0 27.0 40.0 56-57 34.847405163261605 37.0 33.0 40.0 27.0 40.0 58-59 34.704653322630485 37.0 33.0 40.0 27.0 40.0 60-61 34.537409219986706 37.0 33.0 40.0 27.0 40.0 62-63 34.33860724859093 37.0 33.0 40.0 27.0 40.0 64-65 34.16251637794868 37.0 33.0 40.0 22.0 40.0 66-67 33.93383291471383 37.0 33.0 38.5 22.0 40.0 68-69 33.710015277454104 37.0 33.0 37.0 22.0 40.0 70-71 33.48089109850937 37.0 33.0 37.0 22.0 40.0 72-73 33.26636905840821 37.0 33.0 37.0 22.0 40.0 74-75 33.040137238147025 37.0 33.0 37.0 22.0 40.0 76-77 32.805823213098904 37.0 33.0 37.0 22.0 37.0 78-79 32.58145299809247 37.0 33.0 37.0 22.0 37.0 80-81 32.37854838292033 37.0 33.0 37.0 22.0 37.0 82-83 32.18131886722425 33.0 33.0 37.0 22.0 37.0 84-85 32.011970446326075 33.0 33.0 37.0 22.0 37.0 86-87 32.03720983626367 33.0 33.0 37.0 22.0 37.0 88-89 32.06367228566249 33.0 33.0 37.0 22.0 37.0 90-91 32.08589364475172 33.0 33.0 37.0 22.0 37.0 92-93 32.0652578609838 33.0 33.0 37.0 22.0 37.0 94-95 32.10522730607559 33.0 33.0 37.0 22.0 37.0 96-97 32.07277816618763 33.0 33.0 37.0 22.0 37.0 98-99 31.99920747127925 33.0 33.0 37.0 22.0 37.0 100 31.4579051762086 33.0 33.0 37.0 22.0 37.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.736223901082159 1101 2 4.262535639849919 1101 3 2.450903073011869 1101 4 1.2912387573975863 1101 5 0.6772222881954661 1101 6 0.7817457996054529 1101 7 0.7721009814244368 1101 8 0.6735255430764866 1101 9 0.7555435543338689 1101 10-11 0.7716457245194235 1101 12-13 0.7986482474998695 1101 14-15 1.1706492841067657 1101 16-17 1.172803876535859 1101 18-19 1.1648779582860058 1101 20-21 1.1646948402524515 1101 22-23 1.0904608766776107 1101 24-25 1.065581787349764 1101 26-27 1.0549782925179514 1101 28-29 0.9658031230764408 1101 30-31 0.9469268551867174 1101 32-33 1.1794146920887698 1101 34-35 1.227416581009642 1101 36-37 1.1444918376642619 1101 38-39 1.2006189863874965 1101 40-41 1.1968723414561069 1101 42-43 1.1916036404040682 1101 44-45 1.1825562595291714 1101 46-47 1.1894126149595081 1101 48-49 1.1759861801200486 1101 50-51 1.0566574689234542 1101 52-53 1.0160407417902064 1101 54-55 1.10582463586249 1101 56-57 1.0548610614021072 1101 58-59 1.0632206938372377 1101 60-61 1.0634318395250162 1101 62-63 1.0843458820166916 1101 64-65 1.0475993886347439 1101 66-67 0.9823843960806755 1101 68-69 0.9299219111627366 1101 70-71 0.9930072666645273 1101 72-73 0.8856502922844669 1101 74-75 0.8525339023979086 1101 76-77 0.8508274832259559 1101 78-79 0.880843741733564 1101 80-81 0.8996720934750329 1101 82-83 0.8307844352997122 1101 84-85 0.9002094258164846 1101 86-87 0.9833465430273698 1101 88-89 0.9123935074259997 1101 90-91 0.9143991499370223 1101 92-93 0.9885391383957902 1101 94-95 0.9397879253747519 1101 96-97 0.8771283639378318 1101 98-99 0.8818549407652831 1101 100 1.0353577860989773 1102 1 0.4975653807448879 1102 2 4.062283255906561 1102 3 2.3336237186275106 1102 4 1.1169514660521074 1102 5 0.48629059935479546 1102 6 0.563057967709149 1102 7 0.5440673302319894 1102 8 0.5813079523343703 1102 9 0.6124589885827092 1102 10-11 0.685804159206036 1102 12-13 0.7107624864461783 1102 14-15 1.0185289544468858 1102 16-17 0.91063520183706 1102 18-19 0.9399884476367717 1102 20-21 0.9388310057675966 1102 22-23 0.8908954110235285 1102 24-25 0.8883039556437993 1102 26-27 0.8718465237366644 1102 28-29 0.9218116120084758 1102 30-31 0.9208970487572046 1102 32-33 1.058313414942269 1102 34-35 1.0830227021767769 1102 36-37 0.8976462562866132 1102 38-39 0.9672359756716489 1102 40-41 1.0084960876256162 1102 42-43 0.986504190471635 1102 44-45 1.0193190452692846 1102 46-47 1.047706453822073 1102 48-49 0.9236463944370357 1102 50-51 0.891676026566337 1102 52-53 0.9305453954760452 1102 54-55 0.8804621432379633 1102 56-57 0.8462800073279908 1102 58-59 0.8931268587813719 1102 60-61 0.8370501040523166 1102 62-63 0.9166664438850134 1102 64-65 0.904331149802637 1102 66-67 0.8817326897292048 1102 68-69 0.9079633660819368 1102 70-71 0.8278760308206756 1102 72-73 0.8448840043840491 1102 74-75 0.8840030967107069 1102 76-77 0.902339122198903 1102 78-79 0.7838645453270843 1102 80-81 0.7773092772813541 1102 82-83 0.8696542272637728 1102 84-85 0.8616335107819424 1102 86-87 0.8207395272866798 1102 88-89 0.8734355904500397 1102 90-91 0.8881350413700453 1102 92-93 0.9888708919502882 1102 94-95 0.9316817566239095 1102 96-97 0.8576276121153761 1102 98-99 0.9138721089641457 1102 100 0.8688913516407695 1103 1 0.4768575883349371 1103 2 3.9981333228409675 1103 3 2.3334707034660873 1103 4 1.058356855049965 1103 5 0.41287064044940536 1103 6 0.4642012229004564 1103 7 0.4626720592254827 1103 8 0.4060582714402514 1103 9 0.4547025364174644 1103 10-11 0.5055270819045816 1103 12-13 0.5137202866391988 1103 14-15 0.7918764140527585 1103 16-17 0.7153103364841726 1103 18-19 0.7654057758644086 1103 20-21 0.778527855180748 1103 22-23 0.6816150337002753 1103 24-25 0.713909089356946 1103 26-27 0.7471443040050261 1103 28-29 0.6921783113640387 1103 30-31 0.7025002444858544 1103 32-33 0.7587435463204883 1103 34-35 0.9349623211176592 1103 36-37 0.7645812053853334 1103 38-39 0.6984630934450635 1103 40-41 0.7668887668697266 1103 42-43 0.8033066327990923 1103 44-45 0.8569357524079209 1103 46-47 0.7653421127367963 1103 48-49 0.6710420234647714 1103 50-51 0.7091471513756531 1103 52-53 0.7131587169791089 1103 54-55 0.7764639411271901 1103 56-57 0.6156990789633667 1103 58-59 0.6939245067375381 1103 60-61 0.7152341191826039 1103 62-63 0.7640846827318768 1103 64-65 0.6737882028209086 1103 66-67 0.6831267543598187 1103 68-69 0.6389297120861102 1103 70-71 0.7491242407145151 1103 72-73 0.6280209708545854 1103 74-75 0.6744456031706534 1103 76-77 0.6567792081240071 1103 78-79 0.7695480934731549 1103 80-81 0.7091124200851269 1103 82-83 0.7052208736944507 1103 84-85 0.7416951526984406 1103 86-87 0.5764204681042209 1103 88-89 0.6483426605910729 1103 90-91 0.6790091190762233 1103 92-93 0.7554180495730343 1103 94-95 0.6845567449493117 1103 96-97 0.654153833279107 1103 98-99 0.7353438486360098 1103 100 0.72895937567489 1104 1 0.4762887698766356 1104 2 3.649067442801634 1104 3 2.185150099470448 1104 4 1.0355693397099657 1104 5 0.41306167579428177 1104 6 0.38424983448796723 1104 7 0.3828388173604438 1104 8 0.3861858315997111 1104 9 0.45047117498811673 1104 10-11 0.47717953287376247 1104 12-13 0.4468326668729006 1104 14-15 0.6169730750788815 1104 16-17 0.6312928965293025 1104 18-19 0.5850950885564004 1104 20-21 0.6204110920912527 1104 22-23 0.5505609759380476 1104 24-25 0.5934829804944357 1104 26-27 0.5974297613083905 1104 28-29 0.5458921623316897 1104 30-31 0.5317201247386123 1104 32-33 0.6732995344448582 1104 34-35 0.7696100347914054 1104 36-37 0.6877673700435096 1104 38-39 0.6261047644945918 1104 40-41 0.6052030728921522 1104 42-43 0.6804759082177441 1104 44-45 0.7265674686580255 1104 46-47 0.7744517832222684 1104 48-49 0.582429254490819 1104 50-51 0.6207220829649955 1104 52-53 0.5732498613236956 1104 54-55 0.5514607492648196 1104 56-57 0.5330288907673548 1104 58-59 0.6667867688246929 1104 60-61 0.7169379543719785 1104 62-63 0.726881621211831 1104 64-65 0.6229718482898008 1104 66-67 0.5838648368032437 1104 68-69 0.5612273956515423 1104 70-71 0.579933858986827 1104 72-73 0.5181289606101345 1104 74-75 0.5693554402046459 1104 76-77 0.5839742566403103 1104 78-79 0.5568592971959205 1104 80-81 0.5475891776102983 1104 82-83 0.5574164462478706 1104 84-85 0.5611881896745032 1104 86-87 0.6264102732005234 1104 88-89 0.5723972976524969 1104 90-91 0.5850642725523976 1104 92-93 0.601745359338036 1104 94-95 0.5352600027450762 1104 96-97 0.517891084885516 1104 98-99 0.5854056373747447 1104 100 0.48085448440782486 1105 1 0.4998362860779153 1105 2 3.698433525972426 1105 3 2.2051768718649534 1105 4 0.9428549700535527 1105 5 0.38487952551729876 1105 6 0.30298765247312076 1105 7 0.3335297649896276 1105 8 0.2788981968263329 1105 9 0.37030228215824224 1105 10-11 0.33750007407405036 1105 12-13 0.36225678592202826 1105 14-15 0.5927494085006089 1105 16-17 0.5000594446747826 1105 18-19 0.46894473224779176 1105 20-21 0.35729681854141404 1105 22-23 0.4432895012170093 1105 24-25 0.44145543878068594 1105 26-27 0.4760300658650536 1105 28-29 0.3885884231845793 1105 30-31 0.3995007240959936 1105 32-33 0.45764219097505077 1105 34-35 0.5246632394106499 1105 36-37 0.38746693359217943 1105 38-39 0.333683327934601 1105 40-41 0.40208773296446054 1105 42-43 0.43123065822584294 1105 44-45 0.5240157287838016 1105 46-47 0.4630599564915414 1105 48-49 0.27593539512511 1105 50-51 0.26322457962017154 1105 52-53 0.20980262176024667 1105 54-55 0.2880915737573204 1105 56-57 0.22504674228269295 1105 58-59 0.3602118042906426 1105 60-61 0.370990765582107 1105 62-63 0.3885348006643454 1105 64-65 0.3773890308982857 1105 66-67 0.24946866517930033 1105 68-69 0.265973097939316 1105 70-71 0.35982747679223337 1105 72-73 0.3243499152691385 1105 74-75 0.25361319216451506 1105 76-77 0.24664543984938092 1105 78-79 0.276390572425278 1105 80-81 0.2551931278429507 1105 82-83 0.34629310440313077 1105 84-85 0.28321398573140755 1105 86-87 0.23077056553123398 1105 88-89 0.20112789014550359 1105 90-91 0.2819693269024093 1105 92-93 0.2881935473997892 1105 94-95 0.29813851717201345 1105 96-97 0.2624899022401159 1105 98-99 0.2963189648231115 1105 100 0.153913452664181 1106 1 0.5224457020300264 1106 2 3.8814755450631893 1106 3 2.1845409877038158 1106 4 0.9279015062127556 1106 5 0.3640962218447541 1106 6 0.2686920239340509 1106 7 0.1874903021195422 1106 8 0.25246747040665696 1106 9 0.25973995243813164 1106 10-11 0.21143757286065323 1106 12-13 0.20185646234978805 1106 14-15 0.3049983520372521 1106 16-17 0.2976305432025228 1106 18-19 0.2554303753476148 1106 20-21 0.32382217042622585 1106 22-23 0.21292014997934672 1106 24-25 0.25391038134660704 1106 26-27 0.27547029883707097 1106 28-29 0.246903660201248 1106 30-31 0.2543434744600148 1106 32-33 0.30171421815511934 1106 34-35 0.44885177848200186 1106 36-37 0.28521701933882326 1106 38-39 0.1734422407318874 1106 40-41 0.2387788185493136 1106 42-43 0.32721198163629595 1106 44-45 0.29942539065081064 1106 46-47 0.2373864538220687 1106 48-49 0.19688109323221425 1106 50-51 0.12364678961855446 1106 52-53 0.15409913041580126 1106 54-55 0.20007146050703284 1106 56-57 0.299511171986623 1106 58-59 0.2459875816729422 1106 60-61 0.2223985377872495 1106 62-63 0.22102114268019335 1106 64-65 0.21111926225243138 1106 66-67 0.14528032025128823 1106 68-69 0.2534489885718969 1106 70-71 0.1509076774070195 1106 72-73 0.094965932094901 1106 74-75 0.02424309671070546 1106 76-77 0.21419936316276278 1106 78-79 0.1810751477367205 1106 80-81 0.2308238154339577 1106 82-83 0.2145210545730052 1106 84-85 0.11782724572169911 1106 86-87 0.17845390479672574 1106 88-89 0.20918049004842842 1106 90-91 0.23843415783591482 1106 92-93 0.30908422528363033 1106 94-95 0.27097364417411285 1106 96-97 0.22124817436437283 1106 98-99 0.2597590165946677 1106 100 0.199982114692979 1107 1 0.4179783356486304 1107 2 3.6416376377484774 1107 3 2.1032251795333003 1107 4 0.8685225428670407 1107 5 0.26615092877193547 1107 6 0.1678323178142378 1107 7 0.08800019393287783 1107 8 0.003624092399228118 1107 9 0.028527499668875578 1107 10-11 0.17712221035459663 1107 12-13 0.13584050885391008 1107 14-15 0.15327864268798663 1107 16-17 0.1316229733656158 1107 18-19 0.16822752051150758 1107 20-21 0.18966108533015102 1107 22-23 0.1819760001327424 1107 24-25 0.11682786883248042 1107 26-27 0.16709435180008825 1107 28-29 0.12126964760637549 1107 30-31 0.10520329684012353 1107 32-33 0.1778473361916113 1107 34-35 0.32855271825270904 1107 36-37 0.11442550634028237 1107 38-39 0.03597324024747195 1107 40-41 0.07727344151072657 1107 42-43 0.09691234010553273 1107 44-45 0.2669775689165945 1107 46-47 0.1118756771387126 1107 48-49 0.043033187532230954 1107 50-51 0.10665970097007715 1107 52-53 0.06369736067577492 1107 54-55 0.13864161390603158 1107 56-57 0.08618807817102692 1107 58-59 0.10783285213959459 1107 60-61 0.06772546819254899 1107 62-63 0.17497782926182026 1107 64-65 0.032155322320257085 1107 66-67 0.0023719527395869022 1107 68-69 0.014650758311923084 1107 70-71 0.03137463041848321 1107 72-73 0.06437126069169352 1107 74-75 0.10904427223145774 1107 76-77 0.06905607235055555 1107 78-79 0.06808466170410554 1107 80-81 0.1640788065529719 1107 82-83 0.08505635571946613 1107 84-85 0.18194840186573202 1107 86-87 0.10744467502117416 1107 88-89 0.028129729512343715 1107 90-91 0.0937457418824188 1107 92-93 0.07738588199281438 1107 94-95 0.04414823484099628 1107 96-97 0.1334027618018041 1107 98-99 0.10221132080910778 1107 100 0.16348749173156918 1108 1 0.4984541378824012 1108 2 3.6993457990973795 1108 3 2.1736998948774833 1108 4 0.9977815253851432 1108 5 0.3223519176428056 1108 6 0.23994077293549054 1108 7 0.12987580779137176 1108 8 0.17160926302528878 1108 9 0.11930803679666013 1108 10-11 0.1730679419291974 1108 12-13 0.1551112314822376 1108 14-15 0.13577929467984262 1108 16-17 0.1336708946963725 1108 18-19 0.24690910357896456 1108 20-21 0.24951720158136226 1108 22-23 0.13061266316037035 1108 24-25 0.19281819409625456 1108 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0.06382804184294244 1108 92-93 0.18223298387125908 1108 94-95 0.14759500860932206 1108 96-97 0.033645311287205715 1108 98-99 0.0533159042764062 1108 100 0.05667717780210069 1109 1 0.6086591921718458 1109 2 3.9381002700718355 1109 3 2.38748612779375 1109 4 1.1546898009204654 1109 5 0.47693523948571226 1109 6 0.47806804607171927 1109 7 0.3598656462911052 1109 8 0.35844447490337217 1109 9 0.41247165773042127 1109 10-11 0.3915806098720722 1109 12-13 0.45143736861059836 1109 14-15 0.5028344226010688 1109 16-17 0.37236573517761684 1109 18-19 0.40709092187130835 1109 20-21 0.38442223960643673 1109 22-23 0.3665785934245278 1109 24-25 0.3367626815887448 1109 26-27 0.33685982495259736 1109 28-29 0.28528024961666887 1109 30-31 0.22742218770802936 1109 32-33 0.38326689888842935 1109 34-35 0.6154906715985717 1109 36-37 0.46024708506002554 1109 38-39 0.3902411891926292 1109 40-41 0.4006592751387359 1109 42-43 0.4231510477033993 1109 44-45 0.3886760582398807 1109 46-47 0.362580199930683 1109 48-49 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0.1365007248010599 2211 26-27 0.1528425636454287 2211 28-29 0.18125472043021063 2211 30-31 0.09994498765065885 2211 32-33 0.208160808498576 2211 34-35 0.24006778644615423 2211 36-37 0.043094610180034465 2211 38-39 0.017642459746319616 2211 40-41 -0.008750499519379673 2211 42-43 0.11502490348796357 2211 44-45 0.09719956685787423 2211 46-47 0.0938488132048434 2211 48-49 0.07882183987233304 2211 50-51 -0.026618775339137812 2211 52-53 0.019461957693053478 2211 54-55 0.13888404388184483 2211 56-57 0.034135880796981155 2211 58-59 0.007650538367819593 2211 60-61 0.038235192839515264 2211 62-63 0.11598325318293234 2211 64-65 0.10233487200853375 2211 66-67 0.1103526149252616 2211 68-69 0.0815342449183376 2211 70-71 0.00428444196650446 2211 72-73 0.12804388132336442 2211 74-75 -0.038177092438125726 2211 76-77 0.02602554534295365 2211 78-79 -0.02626235350768269 2211 80-81 0.060717334597718775 2211 82-83 -0.2953163968953838 2211 84-85 -0.14424833416879324 2211 86-87 0.05925924078477962 2211 88-89 0.12212440244266176 2211 90-91 0.20998732856761748 2211 92-93 0.17607182110244324 2211 94-95 0.15170192690184336 2211 96-97 0.163549657689412 2211 98-99 0.1272565576598801 2211 100 0.004986793637726805 2212 1 0.5843123686966933 2212 2 4.208997520371831 2212 3 2.4589903704198655 2212 4 1.143350395101642 2212 5 0.619671530486734 2212 6 0.5740411597365167 2212 7 0.43734116631706854 2212 8 0.5346442605301149 2212 9 0.2909715573764089 2212 10-11 0.4524291777989262 2212 12-13 0.3827749808683123 2212 14-15 0.4961252656175006 2212 16-17 0.5397174567827747 2212 18-19 0.5481246963352717 2212 20-21 0.5051406889447492 2212 22-23 0.4931295326953986 2212 24-25 0.4992930973959986 2212 26-27 0.4128520272321268 2212 28-29 0.49704588242347114 2212 30-31 0.4612689065587787 2212 32-33 0.6201191564267248 2212 34-35 0.49362511181533364 2212 36-37 0.5950283773635192 2212 38-39 0.6506540925837427 2212 40-41 0.5781911642283291 2212 42-43 0.5703655618832073 2212 44-45 0.7194856375643965 2212 46-47 0.6338792933282491 2212 48-49 0.5873828216272798 2212 50-51 0.5465890118407728 2212 52-53 0.47971987115654 2212 54-55 0.41908775680333576 2212 56-57 0.5790094435915663 2212 58-59 0.4732458532778807 2212 60-61 0.5563807600094748 2212 62-63 0.7571929945320477 2212 64-65 0.7835814844746594 2212 66-67 0.6730847646957372 2212 68-69 0.6285047910410313 2212 70-71 0.7541827391354161 2212 72-73 0.7461066728356371 2212 74-75 0.7827685288094699 2212 76-77 0.7553005977306597 2212 78-79 0.5929349008231455 2212 80-81 0.5117462845697602 2212 82-83 0.6629042644495513 2212 84-85 0.7164869988081222 2212 86-87 0.7179758801053673 2212 88-89 0.7432407369620115 2212 90-91 0.6937783553667778 2212 92-93 0.8014634845428823 2212 94-95 0.7099622861494268 2212 96-97 0.6035089151051132 2212 98-99 0.6589323499280049 2212 100 0.5467672998781623 2213 1 -0.5749158743246525 2213 2 -25.77964445493681 2213 3 -14.739620588650865 2213 4 -6.082254421537659 2213 5 -2.168937604099412 2213 6 -1.4644378282826978 2213 7 -1.5337200755487714 2213 8 -1.2021458629266775 2213 9 -1.2553460245733632 2213 10-11 -1.3604401282887721 2213 12-13 -1.2519832749244273 2213 14-15 -1.5229804328560164 2213 16-17 -1.56319776550027 2213 18-19 -1.5418777363108376 2213 20-21 -1.539716089015485 2213 22-23 -1.528937912418023 2213 24-25 -1.5887020981279392 2213 26-27 -1.5502392578131818 2213 28-29 -1.6861451411123838 2213 30-31 -1.5385310082986052 2213 32-33 -1.5196631217463619 2213 34-35 -1.3029348225032251 2213 36-37 -1.6157959855872832 2213 38-39 -1.5825195983485756 2213 40-41 -1.6008704425344291 2213 42-43 -1.5545939953751997 2213 44-45 -1.514562589644754 2213 46-47 -1.4850111159644683 2213 48-49 -1.5856263616117943 2213 50-51 -1.5058959361614157 2213 52-53 -1.7146497694199923 2213 54-55 -1.5876683096079063 2213 56-57 -1.5299600266997473 2213 58-59 -1.592176818983873 2213 60-61 -1.5326477019500189 2213 62-63 -1.4453485124922167 2213 64-65 -1.6320808034290124 2213 66-67 -1.5330838176797386 2213 68-69 -1.8125029326103643 2213 70-71 -1.549048118980501 2213 72-73 -1.6205207674124935 2213 74-75 -1.7059558523861753 2213 76-77 -1.6511884857699144 2213 78-79 -1.5866293514422587 2213 80-81 -1.5175947724149736 2213 82-83 -1.7162497828654146 2213 84-85 -1.6094237230796153 2213 86-87 -1.5548286567796303 2213 88-89 -1.6232377037118297 2213 90-91 -1.3792533298488117 2213 92-93 -1.5611373182303296 2213 94-95 -1.582710887845586 2213 96-97 -1.7476259143055763 2213 98-99 -1.6341218372641144 2213 100 -1.5450681316124424 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 9.0 4 37.0 5 46.0 6 36.0 7 45.0 8 61.0 9 86.0 10 424.0 11 1940.0 12 5598.0 13 10283.0 14 13877.0 15 15652.0 16 17026.0 17 17622.0 18 17881.0 19 19456.0 20 21241.0 21 23462.0 22 26483.0 23 27957.0 24 29336.0 25 31008.0 26 33592.0 27 37962.0 28 44903.0 29 53493.0 30 63833.0 31 78117.0 32 99222.0 33 131866.0 34 180303.0 35 254981.0 36 387400.0 37 647371.0 38 601168.0 39 2646.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 38.50656565357804 20.224307793914647 10.513153403189275 30.755973149318034 2 12.993700640921885 22.360564151878933 36.093437929597464 28.552297277601717 3 15.427466618331218 26.403273000336625 29.206245630389187 28.96301475094297 4 10.82830301446239 18.573296490556306 36.94043133394631 33.657969161035 5 11.587275897533793 38.64625891274381 35.19829190710066 14.568173282621732 6 30.0745022732054 39.23444397578979 17.564842747937604 13.126211003067212 7 26.223419374736867 33.25843263202285 22.687210595881936 17.83093739735835 8 24.904013937494042 36.41156502529003 20.5892003763455 18.09522066087043 9 25.221672358721005 16.65070957259856 21.315914541817257 36.811703526863184 10-11 23.22672431569127 27.37699470955758 29.059975041628945 20.336305933122205 12-13 24.270363170903224 24.69163561608634 29.042464345368106 21.995536867642326 14-15 21.76572623458048 26.434618889096896 26.14900763126754 25.65064724505508 16-17 21.147737419936732 29.59369450873605 27.216771034637343 22.041797036689875 18-19 21.290993563698386 28.712425408558552 29.048330799034538 20.948250228708524 20-21 22.063808016509718 28.025564370392598 28.599630009368905 21.310997603728776 22-23 21.759072762537677 27.78645814897497 28.382695762035386 22.071773326451964 24-25 21.709752404704066 28.01824482718194 28.30462169022436 21.967381077889637 26-27 21.59324456844519 27.7529661192525 28.478491319180677 22.175297993121642 28-29 21.545759762004867 27.72096002652064 28.75695713618505 21.976323075289443 30-31 21.96180503095025 28.09684236481357 28.324594212340354 21.616758391895832 32-33 21.33330594281585 27.961114902242613 28.648887343664736 22.056691811276803 34-35 21.352130766050337 27.841595405199882 28.750076605896975 22.056197222852813 36-37 21.93424702939445 27.96170624884551 28.232707648910072 21.871339072849967 38-39 21.82928336690562 28.045312574450026 28.326487671248014 21.79891638739634 40-41 21.66644942929873 27.862427459263678 28.418135033021517 22.05298807841607 42-43 21.719694255860027 27.76008321212618 28.410311743354466 22.109910788659327 44-45 21.95795083249286 27.752167423237704 28.245662132304577 22.04421961196486 46-47 21.81649199423539 27.966955167821762 28.3362474660685 21.880305371874357 48-49 21.929276406952905 27.929573746283904 28.171848319644273 21.96930152711892 50-51 21.794033482901735 27.908058512112383 28.106640826735074 22.191267178250808 52-53 21.69478694852392 27.92312820731541 27.81045828942372 22.571626554736945 54-55 21.883625324163262 28.179320926421003 27.711027067664755 22.226026681750973 56-57 21.961100777787617 27.74506853574648 27.840580558400024 22.45325012806588 58-59 21.69392247241313 27.49479150210714 28.287215228927877 22.52407079655185 60-61 21.949754105365656 27.93335422380253 27.88492631793395 22.231965352897863 62-63 21.597030008455057 28.010699953261515 27.919225815942227 22.4730442223412 64-65 21.499592952334417 27.835499218278553 28.07325304033959 22.59165478904744 66-67 21.760221532876837 27.747534984009725 27.894470760125877 22.597772722987557 68-69 22.058890821099883 27.756962046299506 27.78227590971481 22.401871222885806 70-71 21.777677627898303 27.67160163831389 27.901687352796944 22.64903338099086 72-73 21.873220443169007 27.741005306079458 27.93173408478331 22.454040165968227 74-75 22.007101866808483 27.66357560777251 27.71891595088264 22.610406574536363 76-77 21.78363522220204 27.76653574494506 27.909877418869165 22.53995161398374 78-79 21.98015774562874 27.78207394600433 27.939550236939574 22.29821807142735 80-81 21.779895146729316 27.64920596937826 27.941052061655075 22.629846822237347 82-83 21.598008553318053 27.705471955185203 27.89019765995692 22.806321831539826 84-85 21.653474248888507 27.68063207587562 27.703300429403875 22.962593245831993 86-87 21.71678902685882 27.602577548739067 27.772820067934724 22.907813356467393 88-89 21.62047939738937 27.374125081550865 28.075907089931857 22.929488431127908 90-91 21.61734338818199 27.501729432357745 27.97060543339566 22.910321746064604 92-93 21.391751064547503 27.710759344418967 27.888347930688145 23.009141660345385 94-95 21.189491094318893 27.503058557721072 28.14201098853863 23.165439359421402 96-97 21.501326820416256 27.44355230185907 28.02180501436612 23.03331586335856 98-99 21.46467153050301 27.415450952135412 27.905782001549852 23.214095515811724 100 21.294137408780443 27.386535899912 28.00306501862583 23.316261672681726 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 36.0 1 29.0 2 19.5 3 19.5 4 26.5 5 42.0 6 99.0 7 213.5 8 337.5 9 398.5 10 349.0 11 300.5 12 339.5 13 402.5 14 485.0 15 591.0 16 678.0 17 785.0 18 946.0 19 1168.5 20 1488.0 21 2027.5 22 2836.0 23 4138.5 24 6153.5 25 9011.5 26 13258.0 27 19248.0 28 26745.5 29 35427.5 30 44636.5 31 53551.0 32 62153.5 33 70599.5 34 78707.5 35 85707.0 36 92134.0 37 98713.5 38 104065.0 39 107011.5 40 108601.5 41 109174.0 42 108679.5 43 107363.0 44 104937.0 45 102324.5 46 100182.0 47 98077.5 48 95372.5 49 95021.0 50 103729.5 51 100980.5 52 85226.5 53 77482.5 54 72398.5 55 67407.5 56 63036.0 57 57796.0 58 52171.0 59 47104.5 60 41691.0 61 35595.5 62 29458.5 63 23981.5 64 19493.5 65 15761.5 66 12344.5 67 9335.5 68 6996.0 69 5367.5 70 4059.5 71 3006.0 72 2316.0 73 1721.0 74 1248.0 75 851.0 76 512.0 77 313.5 78 204.5 79 119.0 80 52.5 81 27.0 82 16.0 83 9.5 84 5.5 85 4.5 86 4.5 87 2.5 88 1.0 89 0.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content warn #Base N-Count 1 0.7804793840682911 2 5.719844290806771 3 0.0 4 0.005316899281010211 5 0.16499650431134935 6 0.05572386649058753 7 0.035768231526795966 8 0.04187921316795705 9 0.04008389652761594 10-11 0.04156848528789801 12-13 0.03966959268753722 14-15 0.04250066892807512 16-17 0.03385207626643189 18-19 0.028241711765365926 20-21 0.02333911632443443 22-23 0.02359805622448363 24-25 0.02038720146387357 26-27 0.013292248202525527 28-29 0.019593119103722693 30-31 0.02518622094478538 32-33 0.0015709020602984714 34-35 0.0037114719007051798 36-37 0.004488291600852775 38-39 0.006456234841226685 40-41 0.007164003901361161 42-43 0.0073366305013939604 44-45 0.008424178081600594 46-47 0.01658941626315199 48-49 0.02566957542487722 50-51 0.022389670024254037 52-53 0.02756846802523801 54-55 0.024184986664595145 56-57 0.015691757942981433 58-59 0.015070302182863357 60-61 0.012688055102410731 62-63 0.01810853034344062 64-65 0.019765745703755493 66-67 0.020784242643949007 68-69 0.02687796162510681 70-71 0.026411869805018258 72-73 0.02382247080452627 74-75 0.024426663904641065 76-77 0.018885350043588215 78-79 0.014966726222843677 80-81 0.013050570962479609 82-83 0.008251551481567795 84-85 0.010944526442079461 86-87 0.009062896501721951 88-89 0.007819984981485797 90-91 0.008406915421597314 92-93 0.004281139680813416 94-95 0.0015018514202853517 96-97 9.321836401771148E-4 98-99 5.006171400951172E-4 100 0.10809877694053878 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 2896425.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 77.2761654643485 #Duplication Level Percentage of deduplicated Percentage of total 1 85.07067929299158 65.73935889209744 2 10.254277179323058 15.848224400533228 3 2.5641369170901003 5.944400060348972 4 0.913382010427773 2.8233063747990346 5 0.41022277421132153 1.5850221488599079 6 0.23281220975360392 1.0794500905824063 7 0.13929769161357886 0.7535074026152896 8 0.09069110018045778 0.5606608370951089 9 0.0612825196885408 0.42621103183714865 >10 0.2519438358825368 3.1731707431478027 >50 0.007211687578593298 0.37101100788882246 >100 0.0028506022493935282 0.38450422659753863 >500 6.734327840212832E-4 0.314219350850402 >1k 4.4895518784954604E-4 0.5818799754325928 >5k 8.979103756990921E-5 0.41507345731430606 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 6871 0.23722347376507247 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 5093 0.17583745479340912 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 3.452532000655981E-5 0.0 3 0.0 0.0 0.0 3.452532000655981E-5 0.0 4 0.0 0.0 0.0 3.452532000655981E-5 0.0 5 0.0 3.452532000655981E-5 0.0 3.452532000655981E-5 0.0 6 0.0 3.452532000655981E-5 0.0 3.452532000655981E-5 0.0 7 0.0 3.452532000655981E-5 0.0 1.7262660003279905E-4 0.0 8 0.0 3.452532000655981E-5 0.0 1.7262660003279905E-4 0.0 9 0.0 3.452532000655981E-5 0.0 1.7262660003279905E-4 0.0 10-11 0.0 3.452532000655981E-5 0.0 1.7262660003279905E-4 0.0 12-13 0.0 3.452532000655981E-5 0.0 2.0715192003935886E-4 0.0 14-15 0.0 3.452532000655981E-5 0.0 2.762025600524785E-4 0.0 16-17 0.0 3.452532000655981E-5 0.0 3.107278800590383E-4 0.0 18-19 0.0 3.452532000655981E-5 0.0 3.452532000655981E-4 0.0 20-21 0.0 3.452532000655981E-5 0.0 3.62515860068878E-4 0.0 22-23 0.0 3.452532000655981E-5 0.0 5.178798000983972E-4 0.0 24-25 0.0 3.452532000655981E-5 0.0 6.387184201213565E-4 0.0 26-27 0.0 3.452532000655981E-5 0.0 9.667089601836747E-4 0.0 28-29 0.0 3.452532000655981E-5 0.0 0.001657215360314871 0.0 30-31 0.0 6.905064001311962E-5 0.0 0.004022199780764218 0.0 32-33 0.0 6.905064001311962E-5 0.0 0.010927263782076181 0.0 34-35 0.0 6.905064001311962E-5 0.0 0.022113467464201557 0.0 36-37 0.0 6.905064001311962E-5 0.0 0.038443943827304344 0.0 38-39 0.0 6.905064001311962E-5 0.0 0.06363016477208973 0.0 40-41 0.0 6.905064001311962E-5 0.0 0.09368445583780005 0.0 42-43 0.0 1.0357596001967943E-4 0.0 0.12323812976341525 0.0 44-45 0.0 1.0357596001967943E-4 1.7262660003279905E-5 0.16257973191089015 0.0 46-47 0.0 1.2083862002295934E-4 3.452532000655981E-5 0.20913712593973605 0.0 48-49 0.0 1.3810128002623924E-4 3.452532000655981E-5 0.2572481593688771 0.0 50-51 0.0 2.0715192003935886E-4 3.452532000655981E-5 0.3097611710988546 0.0 52-53 0.0 2.0715192003935886E-4 3.452532000655981E-5 0.3654505122694356 0.0 54-55 0.0 2.2441458004263877E-4 3.452532000655981E-5 0.42771692690126617 3.452532000655981E-5 56-57 0.0 2.4167724004591867E-4 3.452532000655981E-5 0.5018600516153534 3.452532000655981E-5 58-59 0.0 2.762025600524785E-4 3.452532000655981E-5 0.5831499175707985 3.452532000655981E-5 60-61 0.0 3.107278800590383E-4 3.452532000655981E-5 0.6691007017271291 3.452532000655981E-5 62-63 0.0 3.107278800590383E-4 3.452532000655981E-5 0.7601957585644372 3.452532000655981E-5 64-65 0.0 3.107278800590383E-4 3.452532000655981E-5 0.8609406423435787 3.452532000655981E-5 66-67 0.0 3.279905400623182E-4 3.452532000655981E-5 0.9775499106657346 3.452532000655981E-5 68-69 0.0 3.452532000655981E-4 3.452532000655981E-5 1.1068644967503043 3.452532000655981E-5 70-71 0.0 3.62515860068878E-4 3.452532000655981E-5 1.257205002718869 3.452532000655981E-5 72-73 0.0 3.797785200721579E-4 3.452532000655981E-5 1.409289037347765 3.452532000655981E-5 74-75 0.0 3.797785200721579E-4 3.452532000655981E-5 1.568657914498045 3.452532000655981E-5 76-77 0.0 3.797785200721579E-4 3.452532000655981E-5 1.7492598634523593 3.452532000655981E-5 78-79 0.0 3.797785200721579E-4 3.452532000655981E-5 1.9500591246105112 3.452532000655981E-5 80-81 0.0 3.797785200721579E-4 3.452532000655981E-5 2.1728337519528385 3.452532000655981E-5 82-83 0.0 3.797785200721579E-4 3.452532000655981E-5 2.4164444099191247 3.452532000655981E-5 84-85 0.0 3.797785200721579E-4 3.452532000655981E-5 2.670516239847398 3.452532000655981E-5 86-87 0.0 3.9704118007543785E-4 3.452532000655981E-5 2.94457823005947 3.452532000655981E-5 88 0.0 4.143038400787177E-4 3.452532000655981E-5 3.17184114900265 3.452532000655981E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GAGTACT 4110 0.0 18.560987 12-13 GTATCAA 11740 0.0 18.150997 1 CATGGGG 5065 0.0 17.544456 4 TATACCG 170 0.0019148712 16.594337 5 TCCGTAC 200 2.9866843E-4 16.455482 3 TATCAAC 13090 0.0 15.808206 2 CAACGCA 12795 0.0 15.72755 5 ATCAACG 12855 0.0 15.617026 3 TCAACGC 12880 0.0 15.587254 4 AACGCAG 13045 0.0 15.478146 6 TAGACAG 1375 0.0 15.387475 5 AGTACTT 5150 0.0 14.99506 12-13 GTACTTT 5205 0.0 14.746292 14-15 GTCTAAG 825 0.0 14.517707 1 GGATACG 275 1.2891351E-4 14.517706 1 GTACGAC 195 0.004778019 14.466356 3 ACGCAGA 14050 0.0 14.367516 7 GTCCTAT 805 0.0 14.258462 1 CGCAGAG 14235 0.0 14.114835 8 GTACATG 13875 0.0 14.099179 1 >>END_MODULE