##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1295095_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 216333 Sequences flagged as poor quality 0 Sequence length 100 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 25.843311006642537 27.0 22.0 33.0 6.0 33.0 2 21.83391807999704 27.0 2.0 33.0 2.0 33.0 3 24.430965224907897 27.0 15.0 33.0 15.0 33.0 4 24.180268382539882 33.0 6.0 37.0 2.0 37.0 5 26.44069097178886 33.0 15.0 37.0 2.0 37.0 6 27.088354527510827 33.0 15.0 37.0 2.0 37.0 7 27.08235451826582 33.0 15.0 37.0 6.0 37.0 8 27.816606805249315 33.0 15.0 37.0 6.0 37.0 9 28.50707936375865 33.0 22.0 37.0 6.0 37.0 10-11 28.217872446644755 33.0 18.5 37.0 6.0 37.0 12-13 27.08071815210809 33.0 15.0 37.0 4.0 37.0 14-15 28.818656885449748 33.0 22.0 37.0 6.0 40.0 16-17 29.16140394669329 33.0 22.0 37.0 6.0 40.0 18-19 29.326487405989837 33.0 22.0 37.0 6.0 40.0 20-21 28.831629940878184 33.0 22.0 37.0 6.0 40.0 22-23 29.708553480051584 33.0 27.0 37.0 6.0 40.0 24-25 29.95393906616189 33.0 27.0 37.0 10.5 40.0 26-27 28.329129166608887 33.0 18.5 37.0 4.0 40.0 28-29 29.00178197501075 33.0 22.0 37.0 6.0 40.0 30-31 29.673808434219467 33.0 27.0 37.0 10.5 40.0 32-33 28.236355525971533 33.0 22.0 37.0 6.0 40.0 34-35 27.979850508244233 33.0 22.0 37.0 6.0 40.0 36-37 28.336273245413324 33.0 22.0 37.0 6.0 40.0 38-39 28.322745489592435 33.0 22.0 37.0 6.0 40.0 40-41 27.34278404126971 33.0 15.0 37.0 6.0 40.0 42-43 27.759403327277855 33.0 15.0 37.0 6.0 40.0 44-45 27.540435347358006 33.0 22.0 37.0 6.0 40.0 46-47 27.057356020579384 33.0 15.0 37.0 6.0 40.0 48-49 26.910781064377602 33.0 15.0 37.0 6.0 40.0 50-51 26.987311228522692 33.0 18.5 37.0 6.0 40.0 52-53 27.379648504851318 33.0 18.5 37.0 6.0 40.0 54-55 27.26462675597343 33.0 22.0 37.0 6.0 40.0 56-57 27.072300573652655 33.0 22.0 37.0 6.0 40.0 58-59 26.85968853572964 33.0 22.0 37.0 6.0 40.0 60-61 26.717380150046456 30.0 18.5 37.0 6.0 37.0 62-63 26.377619225915602 27.0 18.5 37.0 6.0 37.0 64-65 26.13583919235623 27.0 15.0 37.0 6.0 37.0 66-67 26.373084550207317 27.0 18.5 37.0 6.0 37.0 68-69 25.781186873939713 27.0 18.5 35.0 6.0 37.0 70-71 25.60122819911895 27.0 15.0 33.0 6.0 37.0 72-73 25.42662931684024 27.0 15.0 33.0 6.0 37.0 74-75 25.44428265682998 27.0 22.0 33.0 6.0 37.0 76-77 24.99687287653756 27.0 15.0 33.0 6.0 37.0 78-79 24.579359136146586 27.0 15.0 33.0 6.0 37.0 80-81 24.604396925110827 27.0 15.0 33.0 6.0 37.0 82-83 24.836610688152064 27.0 15.0 33.0 6.0 37.0 84-85 24.63835614538697 27.0 15.0 33.0 6.0 37.0 86-87 24.155475586248976 27.0 15.0 33.0 6.0 35.0 88-89 24.05769577456976 27.0 15.0 33.0 6.0 33.0 90-91 24.22926922845798 27.0 15.0 33.0 6.0 37.0 92-93 23.900368875760982 27.0 15.0 33.0 6.0 35.0 94-95 23.9021161819972 27.0 15.0 33.0 6.0 33.0 96-97 24.145370794099836 27.0 15.0 33.0 6.0 37.0 98-99 24.136280641418555 27.0 15.0 33.0 6.0 37.0 100 23.475332011297397 27.0 15.0 33.0 6.0 33.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 7.396352050985126 1101 2 10.80252744241487 1101 3 8.265824392141784 1101 4 12.318235813996488 1101 5 10.761590171631955 1101 6 9.939487229529913 1101 7 8.958735032420758 1101 8 8.887244713761664 1101 9 7.058795211056406 1101 10-11 7.315940199044594 1101 12-13 8.791088808963178 1101 14-15 7.462807128472559 1101 16-17 7.641423316134038 1101 18-19 7.521085694058289 1101 20-21 6.923920523509274 1101 22-23 7.399046519106999 1101 24-25 7.400166218024058 1101 26-27 8.323899120520128 1101 28-29 8.145560611717723 1101 30-31 5.046551102660892 1101 32-33 6.421641796777749 1101 34-35 8.133235160134323 1101 36-37 7.901173383148691 1101 38-39 7.8008378971589885 1101 40-41 8.545926491512109 1101 42-43 8.33366966371678 1101 44-45 8.267468803306507 1101 46-47 7.248339481107095 1101 48-49 5.311875784904874 1101 50-51 6.480950367260512 1101 52-53 6.0343615651723645 1101 54-55 3.261981313730711 1101 56-57 5.7940167556374895 1101 58-59 6.305436446139915 1101 60-61 6.13889592524869 1101 62-63 5.732659154385718 1101 64-65 6.356975387753632 1101 66-67 5.714864978902874 1101 68-69 6.679161847874607 1101 70-71 5.381986547739796 1101 72-73 4.744846110578262 1101 74-75 4.037910139217761 1101 76-77 4.038837183151703 1101 78-79 3.8597832328461905 1101 80-81 4.074590558721347 1101 82-83 3.4022302861000107 1101 84-85 3.5936623061858235 1101 86-87 3.4302748348425034 1101 88-89 3.389649146086658 1101 90-91 3.552599688271563 1101 92-93 3.182306674230354 1101 94-95 3.4570408371500143 1101 96-97 3.5637820271735485 1101 98-99 3.359077551357398 1101 100 3.678483073030989 1102 1 6.64886737734647 1102 2 10.302888062681728 1102 3 8.162164096433163 1102 4 12.382101663257217 1102 5 10.967423204448398 1102 6 9.404092350337702 1102 7 6.610970878075285 1102 8 7.723829639834509 1102 9 6.692053415167479 1102 10-11 7.884372402434426 1102 12-13 9.53755473034796 1102 14-15 6.120371138569926 1102 16-17 7.577706222733383 1102 18-19 7.218205239676752 1102 20-21 7.039828385031093 1102 22-23 6.602981787769107 1102 24-25 7.187224458197154 1102 26-27 9.23142256083747 1102 28-29 8.312311423113325 1102 30-31 4.680580132592375 1102 32-33 5.140632961581211 1102 34-35 5.277717670051384 1102 36-37 5.677769127829539 1102 38-39 5.369914709275829 1102 40-41 7.756641421191151 1102 42-43 8.487415606738775 1102 44-45 7.196908940342212 1102 46-47 5.810149794846726 1102 48-49 4.02616536297916 1102 50-51 5.907451449121311 1102 52-53 6.922366433545971 1102 54-55 6.431973199774706 1102 56-57 7.35445671236306 1102 58-59 7.09065552295203 1102 60-61 7.136862928494271 1102 62-63 5.920362003285824 1102 64-65 7.230280472499398 1102 66-67 5.257431693652244 1102 68-69 7.284255609143987 1102 70-71 6.212323727689313 1102 72-73 6.21527975644916 1102 74-75 5.258204044735251 1102 76-77 4.288183558918018 1102 78-79 5.085270070206448 1102 80-81 4.899148798894444 1102 82-83 4.661967033305206 1102 84-85 4.098909331068622 1102 86-87 3.9394255741140505 1102 88-89 4.415866239487311 1102 90-91 3.9577881123609977 1102 92-93 4.138815870047157 1102 94-95 3.95260070011431 1102 96-97 4.131425373729623 1102 98-99 4.368011918468827 1102 100 4.161714230622046 1103 1 6.947954218730818 1103 2 11.149112151806008 1103 3 8.244019634740166 1103 4 11.79688570124462 1103 5 10.61400342543278 1103 6 9.34097482682387 1103 7 7.775585981718788 1103 8 8.02583858878894 1103 9 7.0607138114783154 1103 10-11 7.35883592174773 1103 12-13 8.682756081822706 1103 14-15 8.568311965891635 1103 16-17 8.341335115071985 1103 18-19 7.479108353547634 1103 20-21 8.56110954656885 1103 22-23 6.799365679651121 1103 24-25 4.870664326702315 1103 26-27 5.853100946373036 1103 28-29 7.3110512642237175 1103 30-31 6.452089583784044 1103 32-33 7.9372588404963835 1103 34-35 6.834351767394093 1103 36-37 3.6318600784276605 1103 38-39 6.19868833725501 1103 40-41 6.555815012643986 1103 42-43 7.735561894749001 1103 44-45 5.783585956139856 1103 46-47 6.512615323108985 1103 48-49 5.907687598391451 1103 50-51 6.791822739414435 1103 52-53 6.296350544586055 1103 54-55 4.62826577579516 1103 56-57 5.650712762038886 1103 58-59 5.711356828768853 1103 60-61 5.0533454415067816 1103 62-63 2.6428759289547124 1103 64-65 4.651739699967205 1103 66-67 4.13550675529045 1103 68-69 4.9102395375523535 1103 70-71 4.1851526930806 1103 72-73 4.58778911632497 1103 74-75 3.485580540032487 1103 76-77 3.6076749295691 1103 78-79 3.25235070211221 1103 80-81 4.166065062249391 1103 82-83 3.5658114310770976 1103 84-85 3.466834634693864 1103 86-87 2.985859689719934 1103 88-89 2.9400055874920596 1103 90-91 2.5264530744649143 1103 92-93 2.3157357860365657 1103 94-95 2.7826618817415607 1103 96-97 2.8907715521608175 1103 98-99 2.702634327381258 1103 100 3.16637133956187 1104 1 5.7857626055130815 1104 2 9.095287247631735 1104 3 6.638243326874335 1104 4 11.837600881377867 1104 5 9.351851958653963 1104 6 7.809622369766224 1104 7 4.291329120979139 1104 8 6.349920716932552 1104 9 6.425745307315509 1104 10-11 6.4320338723676045 1104 12-13 6.9584569717354405 1104 14-15 6.6657770050344105 1104 16-17 6.712935810943591 1104 18-19 6.543760847884112 1104 20-21 6.8587356494010905 1104 22-23 5.895750588413559 1104 24-25 5.848495367546537 1104 26-27 8.190077728349198 1104 28-29 6.690745768940925 1104 30-31 5.982357131123393 1104 32-33 6.808383992240358 1104 34-35 7.23719499555013 1104 36-37 6.176229062435997 1104 38-39 7.273800412730321 1104 40-41 7.020679709586165 1104 42-43 7.41495434530675 1104 44-45 6.265517668958996 1104 46-47 6.549196375901552 1104 48-49 5.739459681322526 1104 50-51 5.413552477411127 1104 52-53 5.6026607045027035 1104 54-55 4.134043995396183 1104 56-57 5.546640288157967 1104 58-59 3.5456571814839926 1104 60-61 3.5849129687729047 1104 62-63 3.2722669457110705 1104 64-65 2.0818394925439385 1104 66-67 2.9806179704849214 1104 68-69 3.166983183422527 1104 70-71 1.9676713086019788 1104 72-73 2.300957148289182 1104 74-75 2.734321184478418 1104 76-77 3.528184980516844 1104 78-79 3.880398724900097 1104 80-81 3.3334709331881456 1104 82-83 2.670330773057845 1104 84-85 2.844464465409711 1104 86-87 0.555463578189304 1104 88-89 0.4274072526134063 1104 90-91 1.8132754838247713 1104 92-93 1.9844942096197329 1104 94-95 2.1029229291812683 1104 96-97 2.5668160764733123 1104 98-99 2.4031538414181703 1104 100 0.7438826426961604 1105 1 5.119490184763663 1105 2 -2.795066286035752 1105 3 0.1238525560440813 1105 4 7.695200209888046 1105 5 8.400463434071614 1105 6 7.711245295802193 1105 7 4.694017941722834 1105 8 5.4525279860006215 1105 9 4.373738229965419 1105 10-11 4.092509447402303 1105 12-13 4.269884221440943 1105 14-15 5.344258331608334 1105 16-17 5.48110728884118 1105 18-19 5.723153073291041 1105 20-21 6.60045529286289 1105 22-23 1.2606609531069282 1105 24-25 3.949509755932816 1105 26-27 6.326233258363551 1105 28-29 5.642203445528917 1105 30-31 4.947480674758303 1105 32-33 6.5893390529749 1105 34-35 5.8302735951504765 1105 36-37 6.0692676015641105 1105 38-39 4.021748505804144 1105 40-41 5.068067909869452 1105 42-43 5.017077550335294 1105 44-45 5.572805336533381 1105 46-47 5.070881491051562 1105 48-49 4.604253202971872 1105 50-51 5.6493417402507475 1105 52-53 5.005251758617728 1105 54-55 4.934337772192585 1105 56-57 4.633988809497325 1105 58-59 1.7752060719519882 1105 60-61 3.51440176853254 1105 62-63 -0.7388593735422759 1105 64-65 -0.33492295362480107 1105 66-67 1.173678447490687 1105 68-69 2.5185305191626703 1105 70-71 2.379087589368659 1105 72-73 2.2454018576838415 1105 74-75 2.252782385434145 1105 76-77 1.6993981928421142 1105 78-79 2.675515639480679 1105 80-81 1.049656794702912 1105 82-83 0.4765143930938045 1105 84-85 0.8515108163299097 1105 86-87 -1.0086927911873254 1105 88-89 0.4850561798034967 1105 90-91 0.7048403698670356 1105 92-93 0.7800546508572204 1105 94-95 0.5047556957860948 1105 96-97 1.2859039376733143 1105 98-99 1.4425847919482564 1105 100 1.5399319170770092 1106 1 6.014824824154978 1106 2 9.743313594596621 1106 3 6.606844947496995 1106 4 11.411888897299775 1106 5 9.56529554487393 1106 6 8.76366681842281 1106 7 6.230119814245498 1106 8 6.694042405791958 1106 9 6.506947032188755 1106 10-11 5.623734104562079 1106 12-13 7.419469623964975 1106 14-15 7.695903053463542 1106 16-17 7.095791329116366 1106 18-19 6.901183963081007 1106 20-21 7.682381576520456 1106 22-23 5.625322213301015 1106 24-25 4.669139351814177 1106 26-27 7.31014596509279 1106 28-29 5.913375252900554 1106 30-31 4.693346090039185 1106 32-33 6.935313812645042 1106 34-35 6.357504904093936 1106 36-37 5.236279766127414 1106 38-39 6.801829602892848 1106 40-41 6.357705250978388 1106 42-43 5.619330500355797 1106 44-45 3.658611068001786 1106 46-47 5.460149794846725 1106 48-49 5.099569618298315 1106 50-51 6.236174853376628 1106 52-53 4.703217497375757 1106 54-55 4.662824263604495 1106 56-57 4.077860967682206 1106 58-59 4.134272544228626 1106 60-61 4.060267183813419 1106 62-63 4.304404556477316 1106 64-65 4.2100677065419525 1106 66-67 1.7446657362054339 1106 68-69 2.5778726304205826 1106 70-71 1.727217344710585 1106 72-73 1.5365563521938377 1106 74-75 2.293310427713976 1106 76-77 2.7222261121095066 1106 78-79 2.5086743255255968 1106 80-81 2.900212628681679 1106 82-83 1.172605331177543 1106 84-85 2.338271033196282 1106 86-87 2.6266596166672436 1106 88-89 2.5531002820405 1106 90-91 2.2194902400205727 1106 92-93 2.521794593451414 1106 94-95 0.8643028277738871 1106 96-97 1.3250423950062213 1106 98-99 1.170139578043294 1106 100 1.9574589114731111 1107 1 5.597734016200594 1107 2 10.028239196042875 1107 3 6.152137674516879 1107 4 11.13329064949001 1107 5 9.570566021859047 1107 6 8.191924362575225 1107 7 3.7660999746311674 1107 8 6.102423252220362 1107 9 4.846603083734667 1107 10-11 4.619448655157733 1107 12-13 6.046978547156005 1107 14-15 6.1847200932818325 1107 16-17 6.734765973347578 1107 18-19 6.312916220995998 1107 20-21 5.364766965664295 1107 22-23 5.914890191792647 1107 24-25 4.542242304579208 1107 26-27 6.707851430257573 1107 28-29 6.227093789960772 1107 30-31 4.5524523803094326 1107 32-33 5.099240943781183 1107 34-35 6.599779970148727 1107 36-37 6.324920659837144 1107 38-39 5.928353498503103 1107 40-41 7.456567254408601 1107 42-43 5.9560963650941225 1107 44-45 3.5276858373894484 1107 46-47 3.0508024625331878 1107 48-49 3.932583107381891 1107 50-51 4.501562143044268 1107 52-53 3.9305595453060356 1107 54-55 4.904846699056218 1107 56-57 4.445202088527708 1107 58-59 2.2729621098894093 1107 60-61 1.8688288117742964 1107 62-63 0.12412364978839818 1107 64-65 1.8977096663991766 1107 66-67 3.3625584724961683 1107 68-69 4.02134363584403 1107 70-71 3.313751438253444 1107 72-73 1.6297585030305335 1107 74-75 1.7982541073134755 1107 76-77 2.6603038018142335 1107 78-79 3.1383503093943226 1107 80-81 1.5506808064965405 1107 82-83 1.1386044968012392 1107 84-85 1.8110471885756887 1107 86-87 1.9644916289047636 1107 88-89 2.073403438764185 1107 90-91 1.1546707897818465 1107 92-93 1.9173515396341436 1107 94-95 1.635549293263157 1107 96-97 1.7051772858419874 1107 98-99 1.4632049838746575 1107 100 -0.09107861228343594 1108 1 4.4856037864233045 1108 2 -2.9256694637481377 1108 3 0.1363888940622182 1108 4 6.700613628577695 1108 5 7.244257813197901 1108 6 6.762481344896912 1108 7 4.855868476164375 1108 8 5.55797835483429 1108 9 5.006111007948473 1108 10-11 3.551939970001989 1108 12-13 2.6616180735602377 1108 14-15 4.041293861620307 1108 16-17 5.073360223706537 1108 18-19 4.2806328063596375 1108 20-21 4.956363086672184 1108 22-23 4.180599339854737 1108 24-25 3.4419995278658426 1108 26-27 5.0137908538440605 1108 28-29 6.203402678034362 1108 30-31 4.349625118658636 1108 32-33 4.111524720770845 1108 34-35 3.3446062443867675 1108 36-37 1.2296557116310218 1108 38-39 2.1166435985579106 1108 40-41 5.078333817351631 1108 42-43 3.0204208705950997 1108 44-45 2.8906166414967203 1108 46-47 4.255140284518067 1108 48-49 4.521912255534566 1108 50-51 1.5307429075191514 1108 52-53 -0.08649144233318395 1108 54-55 2.5774016069052443 1108 56-57 2.7886518731539667 1108 58-59 3.119389985729047 1108 60-61 4.5766293725160345 1108 62-63 3.8558576462282765 1108 64-65 4.061153653372575 1108 66-67 3.647757698871864 1108 68-69 3.114144581586153 1108 70-71 2.2319592388141842 1108 72-73 1.4444985824764949 1108 74-75 1.1960772698425188 1108 76-77 2.0048089404370195 1108 78-79 3.2269253097340282 1108 80-81 3.306522621161882 1108 82-83 1.8890427178276958 1108 84-85 0.06487341299014915 1108 86-87 -0.012768436590597787 1108 88-89 0.08466130296322305 1108 90-91 -0.2186550247204373 1108 92-93 0.1432901479256934 1108 94-95 0.979683019749821 1108 96-97 1.1192587881587848 1108 98-99 -1.340290819621142 1108 100 0.44868286865451523 1109 1 3.4691206021548844 1109 2 7.951562440329404 1109 3 3.9783428769557965 1109 4 -3.4821850709995275 1109 5 0.1458610168145249 1109 6 3.982116054464509 1109 7 2.1083672320188356 1109 8 3.7036573327280315 1109 9 1.8247727259390523 1109 10-11 1.1577504784970607 1109 12-13 4.096429498304548 1109 14-15 2.598575717559882 1109 16-17 2.75811699906734 1109 18-19 2.7611915788647217 1109 20-21 3.325953379081266 1109 22-23 3.9837662134914105 1109 24-25 4.217206846697323 1109 26-27 5.757792211939378 1109 28-29 4.871871611603968 1109 30-31 4.3892145273755645 1109 32-33 6.579523437091709 1109 34-35 1.9354381707891655 1109 36-37 2.5991599603298994 1109 38-39 2.9693530452270913 1109 40-41 3.894249113132698 1109 42-43 0.37227685667927446 1109 44-45 2.844562326986029 1109 46-47 3.0372334256716123 1109 48-49 2.0919681141810287 1109 50-51 3.3695272261692395 1109 52-53 2.800644790440632 1109 54-55 3.5057225452932954 1109 56-57 3.931230951974655 1109 58-59 2.3063583169926822 1109 60-61 0.5008783504562864 1109 62-63 1.3803196404889277 1109 64-65 2.7460522845799353 1109 66-67 2.120523701839211 1109 68-69 1.931792379099722 1109 70-71 0.4675143602753451 1109 72-73 0.5956238946756329 1109 74-75 0.9668289197887994 1109 76-77 -0.3427960212619041 1109 78-79 1.45986191179815 1109 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18-19 -18.556007526280695 2212 20-21 -23.56442693411784 2212 22-23 -18.59974161648622 2212 24-25 -25.308520222653907 2212 26-27 -17.19810935405615 2212 28-29 -18.11688006624838 2212 30-31 -6.600270931237411 2212 32-33 -16.93728193203581 2212 34-35 -13.846154670374148 2212 36-37 -17.49482661685131 2212 38-39 -23.432212950298638 2212 40-41 -10.298081983064169 2212 42-43 -13.900626946452714 2212 44-45 -16.654963400083318 2212 46-47 -16.389084247706464 2212 48-49 -16.83617506255275 2212 50-51 -8.854803870027627 2212 52-53 -2.1707825026242453 2212 54-55 -22.93611190660827 2212 56-57 -22.659373074870985 2212 58-59 -12.067046604707542 2212 60-61 -6.297349837463177 2212 62-63 -2.5515103371397068 2212 64-65 -16.4117195275006 2212 66-67 -16.50631298719882 2212 68-69 -14.00187205043048 2212 70-71 -8.699250740395795 2212 72-73 -16.171060669082756 2212 74-75 -6.191157657392409 2212 76-77 -8.825561121933045 2212 78-79 -4.589623546814828 2212 80-81 -2.280595881956618 2212 82-83 -2.535905307120327 2212 84-85 -4.757388541271801 2212 86-87 -5.482148893971054 2212 88-89 -3.341367803065882 2212 90-91 -3.9026799727453856 2212 92-93 -5.1513543427188 2212 94-95 -3.071526959460158 2212 96-97 -5.278404413504415 2212 98-99 -2.9924987198290474 2212 100 -3.052838960867316 2213 1 -20.944749643930127 2213 2 -16.782218320296995 2213 3 -19.874006116332794 2213 4 -18.690238762274696 2213 5 -21.109172540232453 2213 6 -21.742716160300592 2213 7 -10.36560173987106 2213 8 -10.514560739443937 2213 9 -4.963404494175723 2213 10-11 -17.51562759756558 2213 12-13 -16.226228803417072 2213 14-15 -23.564735244408798 2213 16-17 -17.958429299930813 2213 18-19 -18.427007526280693 2213 20-21 -17.588883455856973 2213 22-23 -19.691502486051437 2213 24-25 -7.8873245704799935 2213 26-27 -23.19410935405615 2213 28-29 -24.04088006624838 2213 30-31 -19.371466583411326 2213 32-33 -23.041281932035808 2213 34-35 -17.76852423559154 2213 36-37 -23.59882661685131 2213 38-39 -11.361560776385595 2213 40-41 -16.92045154828156 2213 42-43 -22.796626946452715 2213 44-45 -16.591180791387668 2213 46-47 -16.16838859553255 2213 48-49 -6.3722620190744905 2213 50-51 -22.746803870027627 2213 52-53 -17.38047815479816 2213 54-55 -17.047524950086533 2213 56-57 -16.917525248784028 2213 58-59 -17.302568343837976 2213 60-61 -18.098023750506655 2213 62-63 -16.668271206704926 2213 64-65 -16.671349962283212 2213 66-67 -8.88885646545969 2213 68-69 -21.51787205043048 2213 70-71 -16.274424653439276 2213 72-73 -8.747278060387103 2213 74-75 -4.133331570435885 2213 76-77 -2.9787567741069587 2213 78-79 -4.401362677249608 2213 80-81 -3.848769795000095 2213 82-83 -4.062535741902938 2213 84-85 -3.737214628228326 2213 86-87 -3.217431502666706 2213 88-89 -2.5974547595876203 2213 90-91 -2.9574408423106036 2213 92-93 -3.5864847775014077 2213 94-95 -3.189744350764503 2213 96-97 -3.4329478917652843 2213 98-99 -4.42256393722035 2213 100 -3.5764911347803583 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 68.0 3 90.0 4 566.0 5 1605.0 6 2005.0 7 2065.0 8 2039.0 9 1941.0 10 2353.0 11 2901.0 12 3809.0 13 4631.0 14 4528.0 15 4939.0 16 5029.0 17 5000.0 18 4806.0 19 4823.0 20 4854.0 21 5096.0 22 5246.0 23 5854.0 24 6191.0 25 6700.0 26 7143.0 27 7302.0 28 7602.0 29 8161.0 30 8933.0 31 10214.0 32 11687.0 33 13453.0 34 14746.0 35 15686.0 36 14452.0 37 8201.0 38 1608.0 39 6.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.0540231860035 21.37731329404987 10.95270823436663 22.615955285580004 2 12.147704195353906 27.791123879340233 31.90351181336371 28.157660111942146 3 22.74041098259327 34.6439847227918 24.471696155499448 18.14390813911548 4 14.143501011479835 25.999643368811988 25.51450197684357 34.34235364286461 5 10.637924431963237 44.57865288060591 31.347332141945365 13.436090545485492 6 30.79680843940104 31.67041831284093 18.276783920633367 19.25598932712466 7 21.65544904821984 37.79022131967448 20.607912819249734 19.946416812855944 8 27.40531759409677 36.484745400194534 20.192293002388382 15.917644003320314 9 26.811994428016323 22.776216427576433 17.749211857572277 32.66257728683497 10-11 21.468946240520097 34.94079960972793 20.765560480970503 22.82469366878147 12-13 26.46289789169078 31.974989665150886 21.34766432410087 20.21444811905746 14-15 27.760519910671714 30.478257596667753 19.973653175419667 21.78756931724086 16-17 20.93237492870015 34.24891598984387 27.15754617454204 17.66116290691394 18-19 23.982840706209085 32.788137274350326 26.545824241221982 16.68319777821861 20-21 21.44621603413429 32.669554926990266 25.4553804151727 20.42884862370274 22-23 19.317017166959026 33.91410607643337 20.726737883595796 26.042138873011805 24-25 21.27999139698748 35.392842391344196 18.11447383535496 25.212692376313367 26-27 24.13192877496551 29.638072643346185 20.404347770027208 25.8256508116611 28-29 23.74414845082715 28.674078628952277 25.142569524338477 22.439203395882096 30-31 26.428673515611184 32.17849244088728 22.41620297810389 18.97663106539765 32-33 18.47907619927235 32.298071673138644 25.819373076903453 23.403479050685554 34-35 15.660621988173782 33.79540691036905 26.4481122766346 24.09585882482257 36-37 24.034136921447704 31.75954650221142 22.369650532610727 21.836666043730144 38-39 26.6133563008447 28.319585000829107 21.769884345710544 23.29717435261565 40-41 21.356826629117986 32.72849513516256 21.998959800078648 23.9157184356408 42-43 26.058449973436343 30.195429065108264 22.50736086354225 21.23876009791314 44-45 28.063085092385947 28.07351155972504 21.64584269439776 22.217560653491255 46-47 21.880830180659924 30.9884526901303 28.193425471294812 18.93729165791496 48-49 24.565054865549257 29.92813215965272 27.213312432171712 18.29350054262631 50-51 20.61117478723748 30.61492905429764 26.65728514279889 22.116611015665985 52-53 18.921554122392354 31.65242531476096 21.755676126675674 27.670344436171014 54-55 20.88144445418847 33.72772272403948 19.083671938183908 26.30716088358814 56-57 23.887573014799937 28.220797727725927 20.837609248622385 27.054020008851754 58-59 23.44986156253762 26.63223787167449 25.620801733477787 24.2970988323101 60-61 25.92468178379907 29.938543854264594 22.923891529781944 21.2128828321544 62-63 18.814603874205197 30.907065821420225 25.547955333590917 24.730374970783668 64-65 16.343241999176296 32.14041718148121 26.431668968190518 25.084671851151974 66-67 23.33405377674646 30.351726807857744 22.69824267225921 23.61597674313659 68-69 26.588614865690612 27.272993913914895 21.781585315180994 24.356805905213502 70-71 21.725743609236908 31.04414140454243 21.931720992112517 25.298393994108142 72-73 25.928345344703207 29.17764216848502 22.4955295146028 22.39848297220897 74-75 27.68070202447849 27.27599293683506 21.39173273804942 23.65157230063704 76-77 22.192494592848515 30.071954907633682 26.9367433499059 20.798807149611903 78-79 24.58302622996371 29.530667558345712 26.35170616035623 19.534600051334337 80-81 21.260215002897453 30.070839788545005 25.65228189268777 23.016663315869767 82-83 19.54471203211052 31.104965929244845 21.884392793801926 27.465929244842712 84-85 21.41313164940381 32.23996699035499 19.61002667967047 26.736874680570725 86-87 23.72603515810933 28.03895208308663 21.334252803105763 26.900759955698273 88-89 23.876631466194738 26.396628206173084 25.336422591950075 24.390317735682103 90-91 25.58733176448767 29.439272921182898 23.02331242827168 21.950082886057753 92-93 20.0058945980882 30.109328549580766 24.90769384292985 24.977083009401188 94-95 17.872364184548957 31.268727550203796 25.62324356516722 25.23566470008003 96-97 23.722643019317378 29.80887333904046 22.589049763650575 23.879433877991588 98-99 25.80976386001185 27.411984714204408 22.052663979649914 24.725587446133833 100 21.791093343250278 30.271941242097434 24.547694310152472 23.389271104499816 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 2832.0 1 2697.5 2 2075.5 3 1259.5 4 750.5 5 469.0 6 329.5 7 269.0 8 265.0 9 275.0 10 249.0 11 255.5 12 326.5 13 380.5 14 415.0 15 482.5 16 549.0 17 623.5 18 697.0 19 722.5 20 765.5 21 789.0 22 799.0 23 897.5 24 974.5 25 1109.5 26 1351.0 27 1549.5 28 1774.0 29 1956.5 30 2104.0 31 2262.5 32 2353.5 33 2396.0 34 2425.0 35 2581.0 36 2731.0 37 2838.5 38 2950.0 39 3100.0 40 3177.0 41 3155.5 42 3224.0 43 3336.5 44 3543.5 45 4138.0 46 5583.5 47 7903.0 48 11443.5 49 17886.0 50 26192.0 51 25528.5 52 15915.0 53 8768.0 54 5485.0 55 3872.5 56 2930.0 57 2303.0 58 1817.0 59 1460.0 60 1213.5 61 998.0 62 782.0 63 653.0 64 556.0 65 472.5 66 390.0 67 322.5 68 267.0 69 199.0 70 159.5 71 118.5 72 83.5 73 65.5 74 53.0 75 38.5 76 30.5 77 18.5 78 7.5 79 4.5 80 6.0 81 4.5 82 3.0 83 4.0 84 2.0 85 0.0 86 0.5 87 1.5 88 1.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.5 100 0.5 >>END_MODULE >>Per base N content fail #Base N-Count 1 8.530829785562073 2 25.34102517877531 3 6.443769558966964 4 24.822842562161114 5 13.089542510851327 6 10.260108259026593 7 8.03853318726223 8 9.229290029722696 9 5.425894338820244 10-11 7.140611926982938 12-13 10.545316710811573 14-15 7.8896885819546725 16-17 8.42497446066943 18-19 6.791844055229669 20-21 8.401168568826762 22-23 6.1872206274585935 24-25 5.433059218889397 26-27 10.373128463988389 28-29 6.784448049996995 30-31 4.5885278713834685 32-33 9.411416658577286 34-35 7.4812904180129705 36-37 7.243693749913328 38-39 6.614108804481979 40-41 8.90086117235928 42-43 6.901397382738648 44-45 6.8979305052858315 46-47 6.4675754508096315 48-49 4.163950945995294 50-51 7.039379105360717 52-53 4.7077884557603324 54-55 5.121271373299496 56-57 4.957172507199549 58-59 4.002163331530557 60-61 4.362487461459879 62-63 3.0924546879116916 64-65 4.598466253414874 66-67 2.6877545265863274 68-69 5.439761848631508 70-71 2.714796170718291 72-73 1.6402952855089143 74-75 0.9159490230339338 76-77 1.260556641843824 78-79 1.8487701830049046 80-81 2.2837477407515268 82-83 0.9582449279582865 84-85 1.4154105014029297 86-87 0.45832119926224846 88-89 0.7444541517013123 90-91 0.31225009591694286 92-93 0.4074736632876168 94-95 0.6513107108023279 96-97 0.6457637068778226 98-99 0.508706484909838 100 0.5607096467020751 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 216333.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 54.69904669009229 #Duplication Level Percentage of deduplicated Percentage of total 1 90.54100225200568 49.5250650955021 2 4.804402023914292 5.2559242124812355 3 1.5032447217407872 2.466781596634023 4 0.7202203284292923 1.5758146148762986 5 0.44701174152824585 1.2225558060436494 6 0.34702347482233303 1.1389111951118918 7 0.24028908790071526 0.9200508826740639 8 0.19441806438640982 0.8507586225015679 9 0.13260199225812805 0.6527882309153943 >10 0.8943134578463283 9.213725184723586 >50 0.1026915004675166 3.8638200059642602 >100 0.04785623322758055 5.419164347108942 >500 0.013958068024710992 4.931619522079718 >1k 0.00997004858907928 10.163182728461532 >5k 9.97004858907928E-4 2.799837954921738 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 5134 2.3731931790341743 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 4078 1.8850568336777098 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2520 1.164870824146108 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 2000 0.9245006540842128 No Hit CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC 1962 0.9069351416566127 No Hit GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC 1622 0.7497700304622965 No Hit ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC 1425 0.6587067160350015 No Hit ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG 1393 0.6439147055696542 No Hit GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT 1275 0.5893691669786857 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG 1182 0.5463798865637698 No Hit TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG 1179 0.5449931355826434 No Hit GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT 991 0.4580900740987274 No Hit GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC 776 0.3587062537846746 No Hit CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC 756 0.34946124724383243 No Hit GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT 723 0.3342069864514429 No Hit ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA 703 0.3249619799106008 No Hit GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC 671 0.3101699694452534 No Hit TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC 594 0.2745766942630112 No Hit CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA 586 0.27087869164667433 No Hit CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTC 584 0.2699541909925901 No Hit GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG 567 0.26209593543287435 No Hit CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT 544 0.25146417791090586 No Hit GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG 532 0.2459171739864006 No Hit CTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT 510 0.23574766679147424 No Hit GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA 506 0.23389866548330585 No Hit ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC 496 0.22927616221288474 No Hit CNCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 462 0.21355965109345318 No Hit GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG 452 0.20893714782303208 No Hit AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG 379 0.1751928739489583 No Hit CCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA 367 0.16964587002445303 No Hit CCCNTGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 330 0.1525426079238951 No Hit GNACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 326 0.1506936066157267 No Hit TTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTG 325 0.1502313562886846 No Hit ANAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 308 0.14237310072896878 No Hit GTANATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 303 0.14006184909375824 No Hit ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA 295 0.1363638464774214 No Hit TGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATG 285 0.13174134320700032 No Hit ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG 278 0.12850559091770558 No Hit GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 243 0.11232682947123185 No Hit GTACATGGTAAGCAGTGGTATCAACGCAGAGTACATGGTAAGCAGTGGTA 229 0.10585532489264236 No Hit AAANAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 228 0.10539307456560025 No Hit GGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC 225 0.10400632358447393 No Hit ACCATGTACTCTGCGTTGATACCACTGCTTACCATGTACTCTGCGTTGAT 224 0.10354407325743184 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 4.622503270421064E-4 0.0 0.0 0.0 0.0 9 4.622503270421064E-4 0.0 0.0 0.0 0.0 10-11 4.622503270421064E-4 0.0 0.0 0.0 0.0 12-13 4.622503270421064E-4 0.0 0.0 0.0 0.0 14-15 4.622503270421064E-4 0.0 0.0 0.0 0.0 16-17 4.622503270421064E-4 0.0 0.0 0.0 0.0 18-19 4.622503270421064E-4 0.0 0.0 0.0 0.0 20-21 4.622503270421064E-4 0.0 0.0 0.0 0.0 22-23 4.622503270421064E-4 0.0 0.0 9.245006540842128E-4 0.0 24-25 4.622503270421064E-4 0.0 0.0 0.0018490013081684255 0.0 26-27 4.622503270421064E-4 0.0 0.0 0.003004627125773691 0.0 28-29 4.622503270421064E-4 0.0 0.0 0.006702629742110543 0.0 30-31 4.622503270421064E-4 0.0 0.0 0.02380589184266848 0.0 32-33 4.622503270421064E-4 0.0 0.0 0.05916804186138962 0.0 34-35 4.622503270421064E-4 0.0 0.0 0.12873671608122664 0.0 36-37 4.622503270421064E-4 0.0 0.0 0.2045457697161321 0.0 38-39 4.622503270421064E-4 0.0 0.0 0.32080172696722187 0.0 40-41 4.622503270421064E-4 0.0 0.0 0.5239607457022275 0.0 42-43 4.622503270421064E-4 0.0 0.0 0.6506173353117648 0.0 44-45 4.622503270421064E-4 0.0 0.0 0.7638686654370808 0.0 46-47 4.622503270421064E-4 0.0 0.0 0.9434529174929391 0.0 48-49 4.622503270421064E-4 0.0 0.0 1.081434640115008 0.0 50-51 4.622503270421064E-4 0.0 0.0 1.2288924944414399 0.0 52-53 4.622503270421064E-4 0.0 0.0 1.446612398478272 0.0 54-55 4.622503270421064E-4 0.0 0.0 1.6620210508798934 0.0 56-57 4.622503270421064E-4 0.0 0.0 1.858939690199831 0.0 58-59 4.622503270421064E-4 0.0 0.0 2.090989354374968 0.0 60-61 4.622503270421064E-4 0.0 0.0 2.287445743367863 0.0 62-63 4.622503270421064E-4 0.0 0.0 2.502623270605964 0.0 64-65 4.622503270421064E-4 0.0 0.0 2.814642241359386 0.0 66-67 4.622503270421064E-4 0.0 0.0 3.1074778235405605 0.0 68-69 4.622503270421064E-4 0.0 0.0 3.5045508544697297 0.0 70-71 4.622503270421064E-4 0.0 0.0 4.187987963001484 0.0 72-73 4.622503270421064E-4 0.0 0.0 4.692534194967942 0.0 74-75 4.622503270421064E-4 0.0 0.0 5.122426999117102 0.0 76-77 4.622503270421064E-4 0.0 0.0 5.6473122454734135 0.0 78-79 4.622503270421064E-4 0.0 0.0 6.118576453892841 0.0 80-81 4.622503270421064E-4 0.0 0.0 6.637221320834085 0.0 82-83 4.622503270421064E-4 0.0 0.0 7.420504500006934 0.0 84-85 4.622503270421064E-4 0.0 0.0 8.108332986645587 0.0 86-87 4.622503270421064E-4 0.0 0.0 8.696315402643148 0.0 88 4.622503270421064E-4 0.0 0.0 9.272279310137613 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTGTGCG 80 2.566594E-9 50.203632 9 GTCCCTC 30 0.008542606 49.816917 7 TAAAGGT 35 0.009620367 48.33861 4 TACTGTG 85 4.7020876E-9 46.886513 7 GAATAAT 55 6.784378E-4 45.303684 1 GTACAAA 85 2.5237605E-6 43.971226 1 AAAAGTA 195 0.0 41.5141 2 AGTACTC 185 0.0 37.897377 5 AAAAAGT 215 0.0 37.665276 1 GTAAAGG 60 0.0017010036 37.58499 3 TGTACTG 120 2.240995E-9 37.55901 5 GTACATA 145 7.2619514E-8 34.368313 1 AGGATAT 60 0.0030429119 33.385784 5 AAGTACT 210 5.456968E-12 32.225742 4 GATCTCC 30 0.0036702445 32.141373 16-17 ACTGTGC 125 1.3214049E-7 32.11252 8 ATCTATA 30 0.0037571983 31.988075 44-45 TCTAAAA 85 0.0057566296 29.304071 2 GAACAAA 135 3.5224566E-6 29.232773 3 TGTGCGT 70 3.5713128E-7 28.683813 10-11 >>END_MODULE