FastQCFastQC Report
Wed 25 May 2016
SRR1295094_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295094_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences800851
Sequences flagged as poor quality0
Sequence length100
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT69930.8731961376086188No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA54330.6784033484380989No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG24820.3099203222571989No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC23610.2948113943792291No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC23220.2899415746499661No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC16970.21189959180921294No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG16500.20603083469958833No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG16500.20603083469958833No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG14620.18255580626108978No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT14180.17706165066910076No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT11790.14721839643079673No Hit
GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC8260.10314028452233936No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC8210.10251594865961333No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGCGAA250.004080568460.0635877
GCGAGCA300.00820046750.3496259
GGTATCG809.1177906E-538.9448781
GTACATA6050.032.9583131
GGGCGAG952.4399029E-531.612417
CTGTGCG1203.416948E-629.3706159
GGCGAGC906.3715386E-427.9657218
GTATCGA1209.852906E-425.9536172
ATCGACG1401.5241737E-424.1552094
TCGGAAG1301.9969055E-423.212176
CGGAAGC1352.66311E-422.2457717
CGCACAG1803.279718E-622.2457717
TCGACGC1352.6779273E-422.2275375
CATGGGG30500.022.1752744
ATCGGAA1603.3508415E-521.8802325
ATGGGCG1653.623185E-621.2171945
TACATCG1804.2429348E-420.7628922
CATCGGA2206.669299E-620.4953294
TATCGAC1400.00432529520.1225813
GGGGCGC1305.8857107E-419.7807494
Produced by FastQC (version 0.11.5)