##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1295094_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 800851 Sequences flagged as poor quality 0 Sequence length 100 %GC 40 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 25.8524969064158 27.0 22.0 33.0 6.0 33.0 2 21.971612696993574 27.0 2.0 33.0 2.0 33.0 3 24.289131186700146 27.0 15.0 33.0 6.0 33.0 4 23.967990300318036 33.0 6.0 37.0 2.0 37.0 5 26.105702558902966 33.0 15.0 37.0 2.0 37.0 6 26.907639498483487 33.0 15.0 37.0 6.0 37.0 7 26.689521521481524 33.0 15.0 37.0 6.0 37.0 8 27.372988233766332 33.0 15.0 37.0 6.0 37.0 9 27.987794233883704 33.0 15.0 37.0 6.0 37.0 10-11 27.7185980912804 33.0 15.0 37.0 6.0 37.0 12-13 26.53105821182717 33.0 15.0 37.0 4.0 37.0 14-15 28.162555831234524 33.0 15.0 37.0 6.0 40.0 16-17 28.63171176660827 33.0 22.0 37.0 6.0 40.0 18-19 28.857149457264832 33.0 18.5 38.5 6.0 40.0 20-21 28.474409097322724 33.0 18.5 37.0 6.0 40.0 22-23 29.344189493426367 33.0 22.0 37.0 6.0 40.0 24-25 29.656731401971154 33.0 24.5 37.0 6.0 40.0 26-27 28.37417384756965 33.0 18.5 37.0 4.0 40.0 28-29 29.14194026104731 33.0 22.0 37.0 6.0 40.0 30-31 29.871102115125034 33.0 27.0 37.0 15.0 40.0 32-33 28.609547219145632 33.0 22.0 37.0 6.0 40.0 34-35 28.262531357268706 33.0 22.0 37.0 6.0 40.0 36-37 28.540577460726155 33.0 22.0 37.0 6.0 40.0 38-39 28.58865444383537 33.0 22.0 37.0 6.0 40.0 40-41 27.640557357111373 33.0 18.5 37.0 6.0 40.0 42-43 28.132350462195838 33.0 22.0 37.0 6.0 40.0 44-45 28.08510696746336 33.0 22.0 37.0 6.0 40.0 46-47 27.761706609594043 33.0 22.0 37.0 6.0 40.0 48-49 27.703956790963613 33.0 22.0 37.0 6.0 40.0 50-51 27.75699474683805 33.0 22.0 37.0 6.0 40.0 52-53 27.998301182117523 33.0 22.0 37.0 6.0 40.0 54-55 28.005619647100396 33.0 22.0 37.0 6.0 40.0 56-57 28.0160129662072 33.0 22.0 37.0 6.0 40.0 58-59 27.91545493481309 33.0 22.0 37.0 6.0 40.0 60-61 27.85988279967185 33.0 22.0 37.0 6.0 40.0 62-63 27.748426361458 33.0 22.0 37.0 6.0 40.0 64-65 27.389729175589466 33.0 22.0 37.0 6.0 40.0 66-67 27.64282619363652 33.0 22.0 37.0 6.0 40.0 68-69 27.16273626429885 33.0 22.0 37.0 6.0 37.0 70-71 27.215405862014283 33.0 22.0 37.0 6.0 37.0 72-73 27.213002168942786 27.0 22.0 37.0 10.5 37.0 74-75 27.267150193981152 27.0 22.0 37.0 6.0 37.0 76-77 26.968682064453937 27.0 22.0 35.0 6.0 37.0 78-79 26.523780953011233 27.0 22.0 33.0 6.0 37.0 80-81 26.36145675038178 27.0 22.0 33.0 6.0 37.0 82-83 26.515153255724222 27.0 22.0 33.0 6.0 37.0 84-85 26.382413207950044 27.0 22.0 33.0 6.0 37.0 86-87 26.060754747137732 27.0 22.0 33.0 6.0 37.0 88-89 25.960325953267212 27.0 22.0 33.0 6.0 37.0 90-91 26.09159506574881 27.0 22.0 33.0 6.0 37.0 92-93 25.91161152324215 27.0 22.0 33.0 6.0 37.0 94-95 25.79286970984615 27.0 22.0 33.0 6.0 37.0 96-97 25.876628111846024 27.0 22.0 33.0 6.0 37.0 98-99 25.888634090486246 27.0 22.0 33.0 6.0 37.0 100 25.42932455600355 27.0 22.0 33.0 6.0 37.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 7.285211569193805 1101 2 11.136012912283366 1101 3 8.342684489968534 1101 4 12.641274425938601 1101 5 10.498039632176045 1101 6 9.883572599929142 1101 7 9.192350437719085 1101 8 8.787387939033614 1101 9 6.81540886387819 1101 10-11 7.519928659679056 1101 12-13 8.7858869124263 1101 14-15 7.6849270119989015 1101 16-17 7.879048421317986 1101 18-19 7.803180173935541 1101 20-21 6.888391209961252 1101 22-23 7.2124951671386235 1101 24-25 7.3362104462993365 1101 26-27 8.531264853473804 1101 28-29 8.124417416945786 1101 30-31 5.612310163659707 1101 32-33 7.00640521855928 1101 34-35 8.650133509929365 1101 36-37 8.280506289555277 1101 38-39 8.098744590736974 1101 40-41 8.828670442121023 1101 42-43 8.354567417037213 1101 44-45 8.20206275902365 1101 46-47 7.340004837954773 1101 48-49 5.3960027242992865 1101 50-51 6.861619995094866 1101 52-53 5.554109170804324 1101 54-55 4.0221078479470975 1101 56-57 6.236023009420617 1101 58-59 6.176934585976639 1101 60-61 6.827978605584285 1101 62-63 6.190982980762133 1101 64-65 6.714544846986932 1101 66-67 5.871175655892589 1101 68-69 6.600444346882185 1101 70-71 6.107776889391591 1101 72-73 5.3341121675002015 1101 74-75 4.712719523827204 1101 76-77 4.945789349628125 1101 78-79 4.959515314432682 1101 80-81 4.85341652230483 1101 82-83 4.3843172404519635 1101 84-85 4.262501485658003 1101 86-87 4.161662285047523 1101 88-89 3.9144708396216004 1101 90-91 3.655867422658286 1101 92-93 3.595957617763599 1101 94-95 3.651811168810699 1101 96-97 3.6768629562357837 1101 98-99 3.483845580068998 1101 100 3.6998036647832677 1102 1 7.107941944620428 1102 2 10.69625650460084 1102 3 8.24146318233285 1102 4 12.452362560851274 1102 5 10.498110568400364 1102 6 9.53209966946002 1102 7 6.420948443474288 1102 8 7.600762094156412 1102 9 5.9633035302771695 1102 10-11 7.139366523561808 1102 12-13 8.785543942473815 1102 14-15 5.980617301766685 1102 16-17 6.8820508304727745 1102 18-19 7.188302973908765 1102 20-21 6.7440875226160095 1102 22-23 6.412235179184389 1102 24-25 6.6626659773172 1102 26-27 8.21836951786516 1102 28-29 7.6917599853724745 1102 30-31 3.9357067373062264 1102 32-33 4.4701537964887486 1102 34-35 4.854524863740512 1102 36-37 5.147570466761994 1102 38-39 4.6998511289153875 1102 40-41 6.195503072784206 1102 42-43 7.014090605152045 1102 44-45 6.622886221514445 1102 46-47 5.696435273610263 1102 48-49 3.927272215030733 1102 50-51 5.173841771177308 1102 52-53 5.8217180846770376 1102 54-55 5.4607871626764 1102 56-57 6.254680909440694 1102 58-59 6.092780132386331 1102 60-61 6.164908271648663 1102 62-63 4.388721386704717 1102 64-65 6.242814605402245 1102 66-67 4.129398569631466 1102 68-69 6.217996043328682 1102 70-71 5.652909392904942 1102 72-73 5.291437001870811 1102 74-75 4.401001997226121 1102 76-77 3.6136622667130496 1102 78-79 4.6214011472641054 1102 80-81 4.682609790055615 1102 82-83 4.003612228071582 1102 84-85 3.2274787325294483 1102 86-87 3.3550287442591937 1102 88-89 3.4698281975818475 1102 90-91 3.518604824786376 1102 92-93 3.2886357948364733 1102 94-95 3.1568938162041285 1102 96-97 3.6467455099398585 1102 98-99 3.34003677326983 1102 100 3.540388554029068 1103 1 6.50040537352622 1103 2 10.473125674738174 1103 3 7.50697965223269 1103 4 11.451746789674456 1103 5 10.048027616553739 1103 6 8.426542928331742 1103 7 7.527848247281121 1103 8 7.425642485165948 1103 9 6.440180616980783 1103 10-11 6.855774123465434 1103 12-13 8.168374459115814 1103 14-15 7.824497451837246 1103 16-17 7.450385770065932 1103 18-19 6.596533105048405 1103 20-21 7.751872605051556 1103 22-23 6.292909636211203 1103 24-25 4.516632004921924 1103 26-27 5.59284874548365 1103 28-29 6.655996036726901 1103 30-31 5.7564803235107505 1103 32-33 7.614779308484067 1103 34-35 6.937403740276498 1103 36-37 3.645863586233304 1103 38-39 5.904975040387523 1103 40-41 6.016410380119076 1103 42-43 6.874918918923431 1103 44-45 5.284800137461165 1103 46-47 5.795919320008224 1103 48-49 5.134463899194156 1103 50-51 5.832469795821925 1103 52-53 5.623425997801331 1103 54-55 4.524347380209875 1103 56-57 5.452959744672857 1103 58-59 5.043376284246726 1103 60-61 4.683588270271869 1103 62-63 2.540154801498371 1103 64-65 4.892682605264568 1103 66-67 3.6834420419062717 1103 68-69 4.9333326694946535 1103 70-71 3.4921268575385405 1103 72-73 4.064209004762077 1103 74-75 3.097320621808784 1103 76-77 3.319190955660833 1103 78-79 2.7042752050206254 1103 80-81 3.7241344174607 1103 82-83 3.3178009521276195 1103 84-85 3.002192015150861 1103 86-87 2.6116726364562126 1103 88-89 2.8745177305044365 1103 90-91 2.39779681872848 1103 92-93 1.9961018052312696 1103 94-95 2.5224946147447262 1103 96-97 2.8802306921929812 1103 98-99 2.6455778533648306 1103 100 2.611394646343115 1104 1 6.478112633260803 1104 2 9.741860493106188 1104 3 7.592207564841594 1104 4 12.100917420111042 1104 5 9.475887807606817 1104 6 8.131824767268423 1104 7 4.338697984726927 1104 8 6.83431643407955 1104 9 6.550251215766906 1104 10-11 7.014957355539099 1104 12-13 7.518834286274668 1104 14-15 7.4293328091585735 1104 16-17 7.1458802456997255 1104 18-19 6.78616019095686 1104 20-21 7.512722359717419 1104 22-23 6.535113169697848 1104 24-25 6.025596593942897 1104 26-27 8.431338911626195 1104 28-29 7.447375745508193 1104 30-31 6.587743748697008 1104 32-33 7.910598175535242 1104 34-35 8.254783839608674 1104 36-37 6.894082601928012 1104 38-39 7.431912029791331 1104 40-41 6.886862003643188 1104 42-43 7.229860954492491 1104 44-45 6.9187327402278775 1104 46-47 6.9627999171722195 1104 48-49 5.8094845890916 1104 50-51 5.80185201942022 1104 52-53 6.033043777991445 1104 54-55 4.965781969310061 1104 56-57 6.205380109662276 1104 58-59 4.312316537884374 1104 60-61 4.590948156702154 1104 62-63 4.062856518096883 1104 64-65 2.9514586174508572 1104 66-67 3.632873797274023 1104 68-69 4.385185220353229 1104 70-71 2.836793857815092 1104 72-73 2.6021317011749012 1104 74-75 3.4321308619562387 1104 76-77 4.108087187946989 1104 78-79 4.370133619652709 1104 80-81 3.006920457230077 1104 82-83 2.923110721995343 1104 84-85 3.192949597744281 1104 86-87 1.045002950539704 1104 88-89 0.5754056999188641 1104 90-91 2.4442121024237373 1104 92-93 2.5454169463789036 1104 94-95 2.7052025098993795 1104 96-97 2.850425702440635 1104 98-99 2.568656895833275 1104 100 1.2290060799093432 1105 1 5.412349116396019 1105 2 -2.8647348879075807 1105 3 0.2839579940227104 1105 4 8.350022207705965 1105 5 8.490555708399686 1105 6 7.532646058055949 1105 7 5.195709781207761 1105 8 5.883899235652688 1105 9 4.548902482408632 1105 10-11 4.804065261356524 1105 12-13 4.605990137146016 1105 14-15 5.4287597166273684 1105 16-17 5.5554810689649265 1105 18-19 5.791239387340536 1105 20-21 6.66779656898759 1105 22-23 1.6731828538007996 1105 24-25 3.8836293019182975 1105 26-27 6.699448176049081 1105 28-29 5.692949043991877 1105 30-31 5.0022101564876635 1105 32-33 6.952146277691753 1105 34-35 5.933958062121082 1105 36-37 6.072729858453894 1105 38-39 3.9061133342023595 1105 40-41 5.073498513875624 1105 42-43 5.427785872732763 1105 44-45 5.864266312012187 1105 46-47 5.0309914604574715 1105 48-49 4.6803168174871 1105 50-51 5.497906004157951 1105 52-53 5.214587815565345 1105 54-55 5.013361415049413 1105 56-57 4.744038749742806 1105 58-59 1.8288829459814053 1105 60-61 4.241593536847859 1105 62-63 -0.9388159521174515 1105 64-65 -0.6268205118025278 1105 66-67 0.7394460299111216 1105 68-69 2.795452410490931 1105 70-71 2.689825835595375 1105 72-73 2.2817559553631384 1105 74-75 2.3924708843761238 1105 76-77 1.3442520329839276 1105 78-79 2.932391519121353 1105 80-81 1.3890233919336161 1105 82-83 0.6270302187496348 1105 84-85 1.015382144906546 1105 86-87 -0.5863935331537959 1105 88-89 1.1138946419733706 1105 90-91 0.9685202808473399 1105 92-93 1.3023060564633937 1105 94-95 0.5639397114853146 1105 96-97 1.5515883296588378 1105 98-99 1.5260737548640861 1105 100 1.7307447612893014 1106 1 6.204381853917479 1106 2 10.260998401359544 1106 3 6.840362742156781 1106 4 11.517974138815788 1106 5 9.777256893597112 1106 6 9.062877313851114 1106 7 6.479670599003164 1106 8 7.237516796037166 1106 9 6.604832141721747 1106 10-11 5.529250477143243 1106 12-13 7.798265697842631 1106 14-15 7.517165254281025 1106 16-17 7.515976400700861 1106 18-19 7.070047671787915 1106 20-21 7.955664820201708 1106 22-23 5.769796870527596 1106 24-25 4.92563449805883 1106 26-27 7.571294687933186 1106 28-29 6.139245646303518 1106 30-31 5.226581753979559 1106 32-33 7.259462186511428 1106 34-35 6.6158287186157985 1106 36-37 5.954121753943532 1106 38-39 7.063584622312742 1106 40-41 6.440058228179698 1106 42-43 5.997648028121233 1106 44-45 4.400002401319703 1106 46-47 6.0578718482401115 1106 48-49 5.935636894235746 1106 50-51 6.561414133455553 1106 52-53 5.5976758010969405 1106 54-55 5.151764887099507 1106 56-57 4.870764676280761 1106 58-59 4.877921522275621 1106 60-61 4.767304563498733 1106 62-63 4.867883718304025 1106 64-65 5.2869669329999525 1106 66-67 2.7859278480094822 1106 68-69 3.1961806505048855 1106 70-71 2.8647227849284143 1106 72-73 2.7164943581466545 1106 74-75 2.8634009568099543 1106 76-77 3.4697913850270403 1106 78-79 3.993184527282782 1106 80-81 3.284228437514596 1106 82-83 1.7280520039819471 1106 84-85 2.213907303958017 1106 86-87 2.9924803915847917 1106 88-89 2.9703524072657217 1106 90-91 2.9858197107407527 1106 92-93 2.873468394543025 1106 94-95 1.3068971508713254 1106 96-97 1.2165474307081645 1106 98-99 1.6888249552092738 1106 100 2.5117517659805166 1107 1 6.003025743503109 1107 2 10.071504615018192 1107 3 5.802176294393316 1107 4 11.05542204162682 1107 5 9.259609089596552 1107 6 7.92150486170452 1107 7 4.030399642948488 1107 8 6.275178788208336 1107 9 5.397845101095442 1107 10-11 4.663336290239432 1107 12-13 6.189495634882622 1107 14-15 6.300121080931984 1107 16-17 6.888667655973922 1107 18-19 6.264086739929137 1107 20-21 5.3698121364838975 1107 22-23 5.672976224205751 1107 24-25 4.830419838638267 1107 26-27 7.388118121217115 1107 28-29 6.585499715901559 1107 30-31 5.197170753080137 1107 32-33 5.040069997606068 1107 34-35 6.681284640276825 1107 36-37 6.510603657691995 1107 38-39 6.475307914981766 1107 40-41 7.7814281467244015 1107 42-43 6.312166517081064 1107 44-45 4.267360424603506 1107 46-47 3.579777370225443 1107 48-49 4.305990585060311 1107 50-51 5.300072464571986 1107 52-53 4.140817657467053 1107 54-55 5.497795049630067 1107 56-57 5.460315151964519 1107 58-59 3.064393671656788 1107 60-61 2.808059766883627 1107 62-63 0.8105074530865757 1107 64-65 2.5828951180542354 1107 66-67 4.2909085926350805 1107 68-69 4.922522826108832 1107 70-71 4.060019153010099 1107 72-73 2.335109364670682 1107 74-75 2.5225580274594464 1107 76-77 3.2343244421977673 1107 78-79 4.006774134447806 1107 80-81 2.195266707571225 1107 82-83 1.8991347387518331 1107 84-85 2.5343781738702305 1107 86-87 2.334662329536883 1107 88-89 2.545607034256186 1107 90-91 1.4040188898537416 1107 92-93 2.1811464750862264 1107 94-95 2.2985796525498365 1107 96-97 2.472031412010182 1107 98-99 1.827577029595826 1107 100 9.997929380389792E-4 1108 1 4.130387987785895 1108 2 -3.1413117756166145 1108 3 -0.02520728220050117 1108 4 6.298110469311833 1108 5 6.480369756524979 1108 6 6.398826450930773 1108 7 4.07593330626279 1108 8 4.78363751327813 1108 9 4.321919696167988 1108 10-11 3.1314616438456753 1108 12-13 2.601785519348063 1108 14-15 3.6226548029874266 1108 16-17 4.4679164185453 1108 18-19 3.5841208716267907 1108 20-21 4.389669735578668 1108 22-23 3.7742393134335543 1108 24-25 2.5485289611382917 1108 26-27 4.445350702148502 1108 28-29 6.085696962487614 1108 30-31 4.456313039594711 1108 32-33 4.103133873965227 1108 34-35 2.5250948692745467 1108 36-37 1.0395568595482203 1108 38-39 2.233029129501343 1108 40-41 4.88769227165136 1108 42-43 2.376548204683278 1108 44-45 3.435932056339798 1108 46-47 4.422651296267247 1108 48-49 4.541531142730204 1108 50-51 1.928204217871535 1108 52-53 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1109 12-13 3.9392061355063674 1109 14-15 2.2440382041082074 1109 16-17 2.3779860103699733 1109 18-19 3.094546549008708 1109 20-21 3.7203318115937343 1109 22-23 3.8482331803265986 1109 24-25 4.157019204044786 1109 26-27 5.722871945049626 1109 28-29 5.0862631264347264 1109 30-31 4.5777976853359235 1109 32-33 6.119656223673211 1109 34-35 1.3448646695657551 1109 36-37 3.1089503925187074 1109 38-39 3.129836851359702 1109 40-41 4.397978087061762 1109 42-43 1.0388957165169792 1109 44-45 3.113752869144374 1109 46-47 3.7081364443698277 1109 48-49 2.2238127632888727 1109 50-51 4.117682433655894 1109 52-53 3.196537473597658 1109 54-55 3.919999041602729 1109 56-57 4.430574684426414 1109 58-59 2.79672573489918 1109 60-61 0.9690780317857275 1109 62-63 1.8280360640319593 1109 64-65 3.1880701736489634 1109 66-67 2.8463460282265522 1109 68-69 1.6866425310499835 1109 70-71 0.6415772969597633 1109 72-73 0.6332181269422001 1109 74-75 1.2154273256099017 1109 76-77 0.22528419703857594 1109 78-79 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-17.61116336648924 2213 56-57 -17.532074771911347 2213 58-59 -17.970005165833378 2213 60-61 -19.27727484427553 2213 62-63 -17.57983755988647 2213 64-65 -17.599707750820578 2213 66-67 -8.93121961069506 2213 68-69 -22.8364157213772 2213 70-71 -17.466238609392214 2213 72-73 -9.372441495134687 2213 74-75 -4.115948942145444 2213 76-77 -2.928923261221975 2213 78-79 -4.313284224792664 2213 80-81 -2.9802095552557297 2213 82-83 -2.943994220365532 2213 84-85 -2.852291141947539 2213 86-87 -3.045038939718161 2213 88-89 -2.8986774222393024 2213 90-91 -2.4961096718909594 2213 92-93 -3.4037401178717026 2213 94-95 -3.5248766325611527 2213 96-97 -3.392651897558707 2213 98-99 -3.363096789778819 2213 100 -3.2366874981491236 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 212.0 3 217.0 4 1166.0 5 4155.0 6 6825.0 7 7705.0 8 6805.0 9 6992.0 10 7712.0 11 9446.0 12 11598.0 13 15372.0 14 13394.0 15 15144.0 16 17057.0 17 18128.0 18 18381.0 19 19036.0 20 19281.0 21 19790.0 22 20758.0 23 22131.0 24 22907.0 25 23932.0 26 25215.0 27 25718.0 28 26401.0 29 27841.0 30 29321.0 31 31286.0 32 35301.0 33 42400.0 34 50660.0 35 58485.0 36 63497.0 37 56926.0 38 19608.0 39 48.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.641705314909004 22.35338240629641 14.127115914832093 20.87779636396249 2 12.71698849229569 24.757818087250506 40.60654253749631 21.918650882957493 3 18.125427156566122 33.80255607824642 29.17001397543042 18.902002789757034 4 11.360445308029119 22.514938979757517 36.91674762772994 29.207868084483422 5 10.204388187344156 43.920499332630435 33.8606005896886 12.014511890336802 6 25.8337979799661 39.207853776398174 20.442648735467657 14.515699508168073 7 21.54212216554596 38.3805369072859 23.316565726214748 16.760775200953393 8 24.00457656474699 38.085754274733986 22.310615789697785 15.599053370821242 9 25.225964729838697 21.989017500471792 21.18651113624396 31.59850663344555 10-11 19.971761953266032 34.54118216565649 26.01510735500267 19.47194852607481 12-13 22.961707706576846 32.48401291519348 26.3626854118287 18.19159396640097 14-15 22.127049061008545 31.79896232591741 25.30161677015648 20.77237184291757 16-17 18.113765320558993 35.295463303508086 28.167565145992114 18.42320622994081 18-19 19.688851205864243 33.665996332165726 29.784182699467 16.860969762503036 20-21 21.66453400925308 31.696119107726073 28.55135078506963 18.08799609795122 22-23 19.9777760350692 32.06604451970583 27.12340547703767 20.832773968187297 24-25 19.794273740799728 32.83180502568933 26.66061252242194 20.713308711089002 26-27 20.815133240268942 30.334720062073718 27.595925026585704 21.254221671071637 28-29 20.295293472034416 30.123904547310925 29.448802897723436 20.131999082931223 30-31 21.468349730833488 31.438557089707352 28.285491676623693 18.807601502835464 32-33 18.47296640740807 31.285484041901622 29.527540089655485 20.714009461034824 34-35 17.589746321380193 31.36187316136436 29.910383474580556 21.13799704267489 36-37 20.62922739029386 31.18146624646421 28.498605865120393 19.690700498121533 38-39 21.3459516298633 29.953461035611905 28.04865534111915 20.651931993405647 40-41 19.277154656238782 31.51644611543932 28.260107037452148 20.946292190869755 42-43 20.893086270029784 30.662829614094804 28.349341525596916 20.0947425902785 44-45 21.83926815834261 29.8511648015698 28.12830652863098 20.181260511456607 46-47 19.485187520116618 30.95771054948621 30.451436825178956 19.10566510521822 48-49 20.262399017239943 30.695700945281917 30.029422940784546 19.012477096693594 50-51 19.119734372512585 31.058122066104538 29.684232916350616 20.13791064503226 52-53 18.15695303396236 31.438083665529366 27.78262674325045 22.62233655725783 54-55 19.061786977072607 32.26242668872811 26.932308600275068 21.743477733924216 56-57 20.25362368746069 30.2622750178569 27.400055489200266 22.08404580548215 58-59 19.940444173703415 29.38564827715817 29.16816258286944 21.50574496626898 60-61 20.93531023351415 30.705961780045914 28.106513898283836 20.252214088156098 62-63 18.417358898902027 31.10693454644531 28.92428853849362 21.551418016159044 64-65 17.34351043089215 31.481418438973424 29.482573280732606 21.692497849401825 66-67 19.99039555194801 30.81790402476383 27.987092339907697 21.204608083380457 68-69 21.451009417358655 30.040094358292368 27.417535443729367 21.09136078061961 70-71 19.519439189626958 31.447821426440786 27.55491578286303 21.477823601069225 72-73 20.910027389752756 30.828885722948655 27.670691188466616 20.59039569883197 74-75 21.749076114552977 30.114507615991247 27.172690034385045 20.96372623507073 76-77 19.91213922786377 30.977555325185303 29.176624284014935 19.933681162935997 78-79 20.753641843326648 30.84656878768877 29.023325858645727 19.376463510338855 80-81 19.635572112254074 31.036432554551606 28.744670208560674 20.583325124633646 82-83 18.758664610129514 31.492770340555754 27.462825951384417 22.28573909793031 84-85 19.557094248754556 31.601670798108522 26.66950110150693 22.17173385162999 86-87 20.518378870090757 30.031471119456533 27.202837109177096 22.247312901275606 88-89 20.547895222241934 29.44373775615354 28.501032352064136 21.50733466954039 90-91 21.279634658573027 30.141607389518988 27.790818228231895 20.78793972367609 92-93 19.403166457649224 30.594139910972352 28.199626900732795 21.803066730645632 94-95 18.59688727287337 30.868756214907744 28.48248274892275 22.051873763296136 96-97 20.78978290045098 30.331637475342443 27.462599956917966 21.415979667288614 98-99 21.47203583628989 29.655972871828173 27.045607827268082 21.826383464613862 100 19.785994999109672 30.780429815739513 27.773500297194335 21.660074887956483 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 715.0 1 742.5 2 675.5 3 492.0 4 376.5 5 362.5 6 404.0 7 544.5 8 756.0 9 984.0 10 1264.0 11 1610.5 12 1948.0 13 2304.0 14 2711.5 15 3011.5 16 3354.5 17 3828.5 18 4245.0 19 4619.5 20 5060.0 21 5612.0 22 6485.5 23 7476.5 24 8423.0 25 9887.5 26 11735.0 27 13719.5 28 16166.0 29 18934.5 30 21643.0 31 23937.5 32 25898.0 33 27870.5 34 29339.5 35 29845.0 36 29945.5 37 29785.0 38 29111.5 39 27723.5 40 26091.0 41 24295.0 42 22137.5 43 20234.5 44 18713.5 45 17628.0 46 17559.0 47 19017.0 48 22302.0 49 29294.0 50 38967.0 51 37651.5 52 25239.0 53 15910.0 54 11088.0 55 8595.0 56 7116.5 57 5809.0 58 4730.5 59 3713.0 60 2840.0 61 2190.0 62 1655.0 63 1234.5 64 897.5 65 665.5 66 502.0 67 374.0 68 273.5 69 208.5 70 163.5 71 118.0 72 95.5 73 78.5 74 63.0 75 53.0 76 45.5 77 32.0 78 21.0 79 17.0 80 13.5 81 6.5 82 6.0 83 6.0 84 2.0 85 2.0 86 2.5 87 0.5 88 0.5 89 0.5 90 0.0 91 0.5 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.5 >>END_MODULE >>Per base N content fail #Base N-Count 1 8.108874185085616 2 25.09730274420585 3 6.274325686051463 4 24.94084417700671 5 12.71622311765859 6 9.973515672703162 7 8.00560903339073 8 9.089580958255656 9 5.381775136698337 10-11 7.139093289513281 12-13 10.472110292676167 14-15 7.788277719575801 16-17 8.298922021699418 18-19 6.92413445197671 20-21 8.222815480033114 22-23 6.057306540167897 24-25 5.60591171141698 26-27 9.880552062743257 28-29 6.594922151561276 30-31 4.482356892855225 32-33 9.276631982728373 34-35 7.404935499862021 36-37 7.187167150943184 38-39 6.307290619603396 40-41 8.867192523952646 42-43 6.820057663660281 44-45 6.711548090718498 46-47 6.116618447126869 48-49 4.046070991982279 50-51 6.6601652492161465 52-53 4.791715312835971 54-55 5.214328258315217 56-57 4.812443263478475 58-59 3.6593573586097787 60-61 4.3794663426779765 62-63 3.299490167334498 64-65 4.633820773152559 66-67 2.622710092139487 68-69 5.355428163291299 70-71 2.6730939962614766 72-73 1.5733263740695835 74-75 0.8980447049451147 76-77 1.170192707507389 78-79 1.8283051404068922 80-81 2.3924550259661284 82-83 0.7880991595190616 84-85 1.3198460138028172 86-87 0.550227195820446 88-89 0.7571945343141233 90-91 0.33632972925050975 92-93 0.3336450850407879 94-95 0.5467933485754529 96-97 0.5863138086860102 98-99 0.4861703363047558 100 0.4236743164458807 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 800851.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 87.14926552059974 #Duplication Level Percentage of deduplicated Percentage of total 1 96.67384795195005 84.25054844062583 2 2.4043825519589412 4.190803468675338 3 0.47688593504959303 1.246807769300294 4 0.15152587657300418 0.5282147540280946 5 0.06533675070116168 0.28470249175543855 6 0.041424137376234495 0.21660498882979556 7 0.027298371702871655 0.16653231306695118 8 0.019774004896136576 0.13786320024792367 9 0.013941338739331156 0.10934796883559346 >10 0.1004203014545421 1.7226057675746722 >50 0.013382173346702228 0.7794668360359485 >100 0.007708297975410173 1.2984221691703122 >500 0.002472472881899754 1.4370861356398157 >1k 0.0013089562315939875 2.056004075340752 >5k 2.9087916257644164E-4 1.5749896208732006 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 6993 0.8731961376086188 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 5433 0.6784033484380989 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 2482 0.3099203222571989 No Hit CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC 2361 0.2948113943792291 No Hit GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC 2322 0.2899415746499661 No Hit ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC 1697 0.21189959180921294 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG 1650 0.20603083469958833 No Hit ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG 1650 0.20603083469958833 No Hit TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG 1462 0.18255580626108978 No Hit GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT 1418 0.17706165066910076 No Hit GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT 1179 0.14721839643079673 No Hit GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC 826 0.10314028452233936 No Hit CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC 821 0.10251594865961333 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 1.2486717254520503E-4 0.0 0.0 0.0 5 0.0 1.2486717254520503E-4 0.0 0.0 0.0 6 0.0 1.2486717254520503E-4 0.0 0.0 0.0 7 0.0 1.2486717254520503E-4 0.0 0.0 0.0 8 0.0 1.2486717254520503E-4 0.0 0.0 0.0 9 0.0 1.2486717254520503E-4 0.0 0.0 0.0 10-11 0.0 1.2486717254520503E-4 0.0 0.0 0.0 12-13 0.0 1.2486717254520503E-4 0.0 0.0 0.0 14-15 0.0 1.2486717254520503E-4 0.0 0.0 0.0 16-17 0.0 1.2486717254520503E-4 0.0 0.0 0.0 18-19 1.2486717254520503E-4 1.2486717254520503E-4 0.0 0.0 0.0 20-21 1.2486717254520503E-4 1.2486717254520503E-4 0.0 1.8730075881780755E-4 0.0 22-23 1.2486717254520503E-4 1.2486717254520503E-4 0.0 4.994686901808201E-4 0.0 24-25 1.2486717254520503E-4 1.2486717254520503E-4 0.0 0.0012486717254520505 0.0 26-27 1.2486717254520503E-4 1.2486717254520503E-4 0.0 0.002372476278358896 0.0 28-29 1.2486717254520503E-4 1.2486717254520503E-4 0.0 0.006617960144895867 0.0 30-31 1.2486717254520503E-4 1.2486717254520503E-4 0.0 0.018293040777872537 0.0 32-33 1.2486717254520503E-4 1.2486717254520503E-4 0.0 0.04495218211627382 0.0 34-35 1.2486717254520503E-4 1.2486717254520503E-4 0.0 0.08828109098945996 0.0 36-37 1.2486717254520503E-4 1.2486717254520503E-4 0.0 0.14547025601516386 0.0 38-39 1.2486717254520503E-4 1.2486717254520503E-4 0.0 0.24648779860423475 0.0 40-41 1.2486717254520503E-4 1.2486717254520503E-4 0.0 0.39969981931720133 0.0 42-43 1.2486717254520503E-4 1.2486717254520503E-4 0.0 0.5157014226116968 0.0 44-45 1.2486717254520503E-4 1.2486717254520503E-4 0.0 0.6369474471530909 0.0 46-47 1.2486717254520503E-4 1.2486717254520503E-4 0.0 0.7906589365562383 0.0 48-49 1.2486717254520503E-4 1.2486717254520503E-4 0.0 0.938189500918398 0.0 50-51 1.2486717254520503E-4 1.2486717254520503E-4 0.0 1.107696687648514 0.0 52-53 1.2486717254520503E-4 1.2486717254520503E-4 0.0 1.3003043012994926 0.0 54-55 1.2486717254520503E-4 1.2486717254520503E-4 0.0 1.4796759946606797 0.0 56-57 1.2486717254520503E-4 1.2486717254520503E-4 0.0 1.6720963075528408 0.0 58-59 1.2486717254520503E-4 1.2486717254520503E-4 0.0 1.9052233186947385 0.0 60-61 1.2486717254520503E-4 1.2486717254520503E-4 0.0 2.1449058563952597 0.0 62-63 1.2486717254520503E-4 1.2486717254520503E-4 0.0 2.386960870374139 1.2486717254520503E-4 64-65 1.2486717254520503E-4 1.2486717254520503E-4 0.0 2.662542720181407 1.2486717254520503E-4 66-67 1.2486717254520503E-4 1.2486717254520503E-4 0.0 2.966469418156436 1.2486717254520503E-4 68-69 1.2486717254520503E-4 1.2486717254520503E-4 0.0 3.3258371407415366 1.2486717254520503E-4 70-71 1.2486717254520503E-4 1.2486717254520503E-4 0.0 3.823682557679269 1.2486717254520503E-4 72-73 1.2486717254520503E-4 1.2486717254520503E-4 0.0 4.282756717541715 1.2486717254520503E-4 74-75 1.2486717254520503E-4 1.2486717254520503E-4 0.0 4.718043681034299 1.2486717254520503E-4 76-77 1.2486717254520503E-4 1.2486717254520503E-4 0.0 5.205962157754689 1.2486717254520503E-4 78-79 1.2486717254520503E-4 1.2486717254520503E-4 0.0 5.700685895378792 1.2486717254520503E-4 80-81 1.2486717254520503E-4 1.2486717254520503E-4 0.0 6.260964898589126 1.2486717254520503E-4 82-83 1.2486717254520503E-4 1.2486717254520503E-4 0.0 6.934685728056779 1.2486717254520503E-4 84-85 1.2486717254520503E-4 1.2486717254520503E-4 0.0 7.580124142942944 1.2486717254520503E-4 86-87 1.2486717254520503E-4 1.2486717254520503E-4 0.0 8.23349162328573 1.2486717254520503E-4 88 1.2486717254520503E-4 1.2486717254520503E-4 0.0 8.794270095186246 1.2486717254520503E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTGCGAA 25 0.0040805684 60.063587 7 GCGAGCA 30 0.008200467 50.349625 9 GGTATCG 80 9.1177906E-5 38.944878 1 GTACATA 605 0.0 32.958313 1 GGGCGAG 95 2.4399029E-5 31.61241 7 CTGTGCG 120 3.416948E-6 29.370615 9 GGCGAGC 90 6.3715386E-4 27.965721 8 GTATCGA 120 9.852906E-4 25.953617 2 ATCGACG 140 1.5241737E-4 24.155209 4 TCGGAAG 130 1.9969055E-4 23.21217 6 CGGAAGC 135 2.66311E-4 22.245771 7 CGCACAG 180 3.279718E-6 22.245771 7 TCGACGC 135 2.6779273E-4 22.227537 5 CATGGGG 3050 0.0 22.175274 4 ATCGGAA 160 3.3508415E-5 21.880232 5 ATGGGCG 165 3.623185E-6 21.217194 5 TACATCG 180 4.2429348E-4 20.762892 2 CATCGGA 220 6.669299E-6 20.495329 4 TATCGAC 140 0.004325295 20.122581 3 GGGGCGC 130 5.8857107E-4 19.78074 94 >>END_MODULE