FastQCFastQC Report
Wed 25 May 2016
SRR1295093_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295093_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences877167
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT33840.3857874270235884No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA26420.3011969214528134No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC13170.15014244721928663No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG12470.14216221084468522No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC9610.10955724508559944No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACGCGA1152.4062447E-630.7229486
ACGCGAT1054.7554975E-528.6686027
TCTACGG800.00669645828.4253753
TACACCG750.00874099226.9154155
CTGTGCG1401.1026708E-525.2612889
GTGTATA2251.0934673E-725.2113021
GTACATA3502.910383E-1123.4104921
AGTACGT1350.00183084923.3356932
CCGCAGA2303.7217706E-621.922871
GCGATGT1454.052683E-420.9058939
TACATAG3401.26165105E-820.3844172
GTTGACG1400.004106805720.303843
CATGGGT7050.020.159844
TCTAGGC2309.091396E-619.7741763
GTACAAG2559.091267E-619.7735671
CATGGGG15450.019.1342334
GATATAT3302.0514926E-719.099471
GTATTAG3453.2733624E-718.2690581
GAGTACG1750.00821285418.0080721
GTGTAAG2802.0358902E-518.0080721
Produced by FastQC (version 0.11.5)