##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1295093_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	877167
Sequences flagged as poor quality	0
Sequence length	100
%GC	45
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	25.868295318907347	27.0	22.0	33.0	6.0	33.0
2	21.819876944755105	27.0	2.0	33.0	2.0	33.0
3	24.190322937365405	27.0	15.0	33.0	6.0	33.0
4	24.022272839721513	33.0	2.0	37.0	2.0	37.0
5	26.108650918240198	33.0	15.0	37.0	2.0	37.0
6	26.986662744950504	33.0	15.0	37.0	2.0	37.0
7	26.752262682020643	33.0	15.0	37.0	6.0	37.0
8	27.551764943277618	33.0	15.0	37.0	6.0	37.0
9	28.48390101314801	33.0	22.0	37.0	6.0	37.0
10-11	28.025095563330584	33.0	18.5	37.0	4.0	37.0
12-13	26.799893292839332	33.0	15.0	37.0	4.0	37.0
14-15	28.540566961593402	33.0	18.5	37.0	4.0	40.0
16-17	28.95270626915969	33.0	22.0	37.0	4.0	40.0
18-19	29.187029949827114	33.0	22.0	38.5	6.0	40.0
20-21	28.614373317737673	33.0	18.5	37.0	6.0	40.0
22-23	29.46284002932167	33.0	24.5	37.0	6.0	40.0
24-25	29.615711147364188	33.0	24.5	37.0	6.0	40.0
26-27	28.108455972465904	33.0	18.5	37.0	4.0	40.0
28-29	28.84923452432661	33.0	22.0	37.0	6.0	40.0
30-31	29.605679420224426	33.0	24.5	37.0	10.5	40.0
32-33	28.23709453274006	33.0	22.0	37.0	4.0	40.0
34-35	27.85632781442986	33.0	15.0	37.0	6.0	40.0
36-37	28.170526821004437	33.0	22.0	37.0	6.0	40.0
38-39	28.297701577920737	33.0	22.0	37.0	6.0	40.0
40-41	27.259228858358785	33.0	15.0	37.0	4.0	40.0
42-43	27.87936390675892	33.0	18.5	37.0	6.0	40.0
44-45	27.780774926553324	33.0	22.0	37.0	6.0	40.0
46-47	27.496266389410454	33.0	15.0	37.0	6.0	40.0
48-49	27.44361506987837	33.0	18.5	37.0	6.0	40.0
50-51	27.367647779727236	33.0	22.0	37.0	6.0	40.0
52-53	27.577316520115325	33.0	22.0	37.0	6.0	40.0
54-55	27.536615034537324	33.0	22.0	37.0	6.0	40.0
56-57	27.493297741479104	33.0	22.0	37.0	6.0	40.0
58-59	27.42693751588922	33.0	22.0	37.0	6.0	40.0
60-61	27.345804732736184	33.0	22.0	37.0	6.0	40.0
62-63	27.30290298198633	33.0	22.0	37.0	6.0	40.0
64-65	26.826911523119314	33.0	22.0	37.0	6.0	38.5
66-67	27.037024876676846	33.0	22.0	37.0	6.0	37.0
68-69	26.544779956382307	30.0	18.5	37.0	6.0	37.0
70-71	26.67181163906075	27.0	22.0	37.0	6.0	37.0
72-73	26.744459720896934	27.0	22.0	37.0	6.0	37.0
74-75	26.8526409452248	27.0	22.0	35.0	6.0	37.0
76-77	26.578022771034476	27.0	22.0	33.0	6.0	37.0
78-79	26.12111091730537	27.0	22.0	33.0	6.0	37.0
80-81	25.901197833479827	27.0	22.0	33.0	6.0	37.0
82-83	26.060549473475405	27.0	22.0	33.0	6.0	37.0
84-85	25.96424056080541	27.0	22.0	33.0	6.0	37.0
86-87	25.69410670944073	27.0	22.0	33.0	6.0	37.0
88-89	25.653270129861248	27.0	22.0	33.0	6.0	37.0
90-91	25.774232842776804	27.0	22.0	33.0	6.0	37.0
92-93	25.604012690855903	27.0	22.0	33.0	6.0	37.0
94-95	25.4250228291762	27.0	22.0	33.0	6.0	37.0
96-97	25.48743055769312	27.0	22.0	33.0	6.0	37.0
98-99	25.50839520866608	27.0	22.0	33.0	6.0	37.0
100	25.13495263729712	27.0	15.0	33.0	6.0	37.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	7.356903996868326
1101	2	11.198989408576136
1101	3	8.297374989265911
1101	4	12.73375332565097
1101	5	10.741637031727727
1101	6	9.92905898161074
1101	7	9.453988304060164
1101	8	9.253831042898998
1101	9	7.3134077673368765
1101	10-11	7.753287855456971
1101	12-13	9.142410979468771
1101	14-15	8.10738956549282
1101	16-17	8.01240522665396
1101	18-19	8.00009397506243
1101	20-21	7.124510787193152
1101	22-23	7.21800009192231
1101	24-25	7.260857350756293
1101	26-27	8.435632738961534
1101	28-29	7.994373373890088
1101	30-31	5.505570080124908
1101	32-33	6.643008382347482
1101	34-35	8.555249386872937
1101	36-37	8.219132358510876
1101	38-39	8.019844375135818
1101	40-41	8.820942134404053
1101	42-43	8.580596217116568
1101	44-45	8.38284386158221
1101	46-47	7.074974250745441
1101	48-49	5.256588416037374
1101	50-51	6.829749564347402
1101	52-53	6.06875469923823
1101	54-55	3.921461687204875
1101	56-57	6.170050069991234
1101	58-59	6.301146328117294
1101	60-61	6.683539904353605
1101	62-63	6.08219605972911
1101	64-65	6.5651384196363125
1101	66-67	5.7682893078834745
1101	68-69	6.724281469219644
1101	70-71	6.263038303222629
1101	72-73	5.563336209843694
1101	74-75	4.911390429013625
1101	76-77	4.88369760598987
1101	78-79	4.966654037129224
1101	80-81	4.644410988657427
1101	82-83	4.547821051120142
1101	84-85	4.333624348569355
1101	86-87	4.047994270320931
1101	88-89	4.017139633920095
1101	90-91	3.9825824794758375
1101	92-93	3.698269836603263
1101	94-95	3.3305141279567003
1101	96-97	3.550215090046745
1101	98-99	3.4261281124302023
1101	100	3.543286118320257
1102	1	6.697093214289438
1102	2	10.491059856425824
1102	3	8.087645213839277
1102	4	12.264310219423326
1102	5	10.597327624938643
1102	6	9.672453337629936
1102	7	6.632856612718836
1102	8	7.648569515448056
1102	9	6.51830374928948
1102	10-11	7.625817253120442
1102	12-13	9.1594340621644
1102	14-15	6.221327514824839
1102	16-17	7.382674212090045
1102	18-19	7.436107010323408
1102	20-21	6.955083228085151
1102	22-23	6.606700954917731
1102	24-25	7.228393691062767
1102	26-27	8.440107240527244
1102	28-29	7.538850440585794
1102	30-31	4.157481796804859
1102	32-33	4.456864919201976
1102	34-35	5.005667248284119
1102	36-37	5.257475261381046
1102	38-39	4.779530925516589
1102	40-41	6.391649102756254
1102	42-43	7.094600929312467
1102	44-45	6.708849299889387
1102	46-47	5.38650537856882
1102	48-49	3.3576994126280084
1102	50-51	5.097025685824548
1102	52-53	5.812882838772033
1102	54-55	5.448084464864273
1102	56-57	6.543018956064721
1102	58-59	6.192242808272571
1102	60-61	6.344847495285439
1102	62-63	4.647997144611175
1102	64-65	5.991797995040713
1102	66-67	3.8055352268369482
1102	68-69	6.114859133017379
1102	70-71	5.354645145808185
1102	72-73	5.03215670749049
1102	74-75	4.154167243017962
1102	76-77	3.2446540983743866
1102	78-79	4.412773713471196
1102	80-81	4.397901997384658
1102	82-83	3.8271237526710316
1102	84-85	3.114807469898267
1102	86-87	3.1964423377300015
1102	88-89	3.3393629530940636
1102	90-91	3.2706132818809195
1102	92-93	3.1750394867728033
1102	94-95	3.169202773290131
1102	96-97	3.405766135554348
1102	98-99	3.122852447233516
1102	100	2.9191497506832818
1103	1	7.076056375959009
1103	2	11.275533239636342
1103	3	8.10828459337463
1103	4	12.185596619453325
1103	5	10.594987581155479
1103	6	9.430666196942536
1103	7	8.456767072863972
1103	8	8.19368545411412
1103	9	7.189243845106176
1103	10-11	7.652811549355281
1103	12-13	8.95443778888994
1103	14-15	8.708837564092615
1103	16-17	8.149852014400146
1103	18-19	7.5034962867292485
1103	20-21	8.775455203343448
1103	22-23	7.265409949217474
1103	24-25	5.4339888186620975
1103	26-27	6.089270895343393
1103	28-29	7.30210448724721
1103	30-31	6.475320946408278
1103	32-33	8.287857769279796
1103	34-35	7.520770008639261
1103	36-37	4.362592653235687
1103	38-39	6.6406188422535735
1103	40-41	7.0276965587387
1103	42-43	7.849420622595918
1103	44-45	5.823350012420562
1103	46-47	6.413575557029752
1103	48-49	5.685687297254212
1103	50-51	6.661333968061896
1103	52-53	6.348878700700368
1103	54-55	5.328863672782745
1103	56-57	6.205184986375421
1103	58-59	5.5762688379269925
1103	60-61	5.212198039527998
1103	62-63	3.0344298713554707
1103	64-65	5.249299788087907
1103	66-67	4.331076188003998
1103	68-69	5.440438855645873
1103	70-71	3.9889752903301314
1103	72-73	4.619712344690246
1103	74-75	3.6276017253078123
1103	76-77	3.8048042397086945
1103	78-79	3.085953506884035
1103	80-81	4.179924296797758
1103	82-83	3.6470584529391665
1103	84-85	3.620113659075294
1103	86-87	2.8873016643880405
1103	88-89	3.0636891247859523
1103	90-91	2.8555975663496156
1103	92-93	2.4383414359205773
1103	94-95	2.75164895843573
1103	96-97	3.0695038654862863
1103	98-99	2.799934315659012
1103	100	2.9379858286679443
1104	1	6.446910100809472
1104	2	9.73937228259447
1104	3	7.299692967490998
1104	4	11.952861437289105
1104	5	9.343220316337643
1104	6	8.145728730928393
1104	7	3.9102744138101357
1104	8	6.301352664554582
1104	9	6.410559777678223
1104	10-11	7.018227536304703
1104	12-13	7.385378517546819
1104	14-15	7.31324213179246
1104	16-17	7.084599159587746
1104	18-19	6.642158603205605
1104	20-21	7.319772767236863
1104	22-23	6.424191895927645
1104	24-25	5.81299915036411
1104	26-27	8.055285033461931
1104	28-29	7.2020501098682
1104	30-31	6.3668380752826685
1104	32-33	7.4238012168056144
1104	34-35	8.007719437676776
1104	36-37	6.587443115443406
1104	38-39	7.313267020611377
1104	40-41	7.230524182447244
1104	42-43	7.4717697253691675
1104	44-45	6.906819289201422
1104	46-47	6.542178058389183
1104	48-49	5.409450211881726
1104	50-51	5.522671651585551
1104	52-53	5.788519809998899
1104	54-55	5.018096951738698
1104	56-57	6.011873403993697
1104	58-59	3.697802280015086
1104	60-61	4.092024031915162
1104	62-63	3.815289033943831
1104	64-65	2.9320077815626213
1104	66-67	3.517590598009434
1104	68-69	4.135653646292962
1104	70-71	2.4957724627064266
1104	72-73	2.454676999393886
1104	74-75	3.2450568639701274
1104	76-77	3.6552664474302823
1104	78-79	4.024507068602357
1104	80-81	3.0483310395927568
1104	82-83	3.015737082629215
1104	84-85	2.788955420902372
1104	86-87	0.7470080265413834
1104	88-89	0.31137808617801355
1104	90-91	2.1280282351913797
1104	92-93	2.253186460390399
1104	94-95	2.262524434237797
1104	96-97	2.6766273021366374
1104	98-99	2.3151599817819033
1104	100	0.8270559754868678
1105	1	5.9299621009394485
1105	2	-2.584985476275353
1105	3	0.6842342061622588
1105	4	8.530537265868372
1105	5	8.84054213696476
1105	6	8.219149119828739
1105	7	5.505877523931659
1105	8	6.338544088744435
1105	9	5.131640630888345
1105	10-11	4.935737684975511
1105	12-13	5.039518833039139
1105	14-15	5.96285598338736
1105	16-17	6.083100402848444
1105	18-19	6.343858564874001
1105	20-21	7.402800209840834
1105	22-23	2.7317109272914024
1105	24-25	4.167793000860538
1105	26-27	7.1517469380964975
1105	28-29	6.1050044607630305
1105	30-31	5.600922339803457
1105	32-33	7.400487814176358
1105	34-35	6.401175719673091
1105	36-37	6.568689964876732
1105	38-39	4.484482436986532
1105	40-41	5.494187613214141
1105	42-43	5.958726055819145
1105	44-45	6.566928176236516
1105	46-47	5.51933668361632
1105	48-49	5.256867602693472
1105	50-51	6.20923825352272
1105	52-53	5.763830869598635
1105	54-55	5.571926387355564
1105	56-57	5.3128943241877735
1105	58-59	1.9781688542890912
1105	60-61	4.494316566110946
1105	62-63	-0.5435368230031159
1105	64-65	-0.3210493284881615
1105	66-67	1.0621705593557742
1105	68-69	3.042910308959687
1105	70-71	2.9374783883454327
1105	72-73	2.6689496092118965
1105	74-75	2.6004107353318275
1105	76-77	1.779725369504419
1105	78-79	3.224195699876855
1105	80-81	1.6904840329797644
1105	82-83	0.983931635746945
1105	84-85	1.297000742477998
1105	86-87	-0.4200743064465904
1105	88-89	1.508002202761272
1105	90-91	1.3218151523563115
1105	92-93	1.7438505746846893
1105	94-95	0.9766607411597121
1105	96-97	2.0489372250162283
1105	98-99	1.7258519662801781
1105	100	1.9538533261496447
1106	1	6.270108645460539
1106	2	10.466327175222848
1106	3	6.768190712682564
1106	4	11.590928704950766
1106	5	9.811032096494472
1106	6	9.591005353222773
1106	7	6.799435045966558
1106	8	7.5387431018917646
1106	9	6.992627824375553
1106	10-11	5.762504338707998
1106	12-13	8.278698889932762
1106	14-15	7.988794458601372
1106	16-17	7.9681880493671216
1106	18-19	7.3441313559160655
1106	20-21	8.313802027609718
1106	22-23	6.379096589014306
1106	24-25	5.234505705380489
1106	26-27	7.655838803727423
1106	28-29	6.388184050573589
1106	30-31	5.435961979009715
1106	32-33	7.5495517065036495
1106	34-35	6.8964836578784485
1106	36-37	6.396283791446436
1106	38-39	7.324883614415803
1106	40-41	6.915931224274733
1106	42-43	6.215908645353412
1106	44-45	4.458795790590159
1106	46-47	6.007572587387152
1106	48-49	6.045845224329923
1106	50-51	6.826626119363723
1106	52-53	5.635922320665397
1106	54-55	5.380766493253802
1106	56-57	5.174237277421081
1106	58-59	4.8839775854461855
1106	60-61	5.108803460547659
1106	62-63	5.044736151296423
1106	64-65	5.309494943416762
1106	66-67	3.1989697496706135
1106	68-69	3.619771315149375
1106	70-71	3.044843522996377
1106	72-73	3.068123637605069
1106	74-75	3.0395995640008913
1106	76-77	3.7018246006331594
1106	78-79	4.1310027093635036
1106	80-81	3.862705971190149
1106	82-83	2.247361880437577
1106	84-85	2.7786293203636845
1106	86-87	3.2751334547309305
1106	88-89	3.454700397535472
1106	90-91	3.0855169309874313
1106	92-93	2.8045769614981992
1106	94-95	1.380868483620386
1106	96-97	1.5561434899635174
1106	98-99	2.13549401917734
1106	100	2.868136318710679
1107	1	6.368073269812523
1107	2	10.547587471855401
1107	3	6.391122712378142
1107	4	11.542199611591645
1107	5	9.545941374208077
1107	6	8.374400298471553
1107	7	4.041334841824625
1107	8	6.639410032689437
1107	9	5.845214181785099
1107	10-11	4.930650866504429
1107	12-13	6.526196920142187
1107	14-15	6.5501693836150245
1107	16-17	7.125231561593253
1107	18-19	6.620858252287174
1107	20-21	5.7168681857122685
1107	22-23	6.199135934461854
1107	24-25	4.959408083231086
1107	26-27	7.536615130649981
1107	28-29	6.706784945261422
1107	30-31	5.37430949259679
1107	32-33	5.101485207045343
1107	34-35	6.910880756466469
1107	36-37	6.737147236833998
1107	38-39	6.611696873792454
1107	40-41	7.983307854947956
1107	42-43	6.44640086356144
1107	44-45	4.157654822975303
1107	46-47	3.6563357044016627
1107	48-49	4.44579665108505
1107	50-51	5.180730390675514
1107	52-53	4.16157786361131
1107	54-55	5.251639586138381
1107	56-57	5.534010152733337
1107	58-59	3.072954928377772
1107	60-61	2.792337997915478
1107	62-63	0.5976702159145084
1107	64-65	2.1562120803709313
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2104	3	7.9908294547123155
2104	4	12.259006081117274
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2206	5	10.015449447896806
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2206	7	8.133099632710493
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2206	14-15	7.662962707438933
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2206	28-29	6.831130880914305
2206	30-31	6.403784632907435
2206	32-33	8.0463925950299
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2206	74-75	1.6262454314402532
2206	76-77	2.393457313825241
2206	78-79	1.105864062376778
2206	80-81	2.247228417095595
2206	82-83	2.8168078503911467
2206	84-85	3.4975586221686683
2206	86-87	-1.449382697359713
2206	88-89	-1.2152587536886834
2206	90-91	0.20900297214305041
2206	92-93	1.7321017662766174
2206	94-95	2.6753505863472995
2206	96-97	1.4656515960831626
2206	98-99	1.313991227059777
2206	100	1.466270141601683
2207	1	6.141539118877795
2207	2	10.545040792786914
2207	3	7.479690933865442
2207	4	11.895099398004763
2207	5	10.255088289829779
2207	6	9.017930395848797
2207	7	5.467286222101137
2207	8	6.5361241248303585
2207	9	5.837591082249016
2207	10-11	6.76549739399513
2207	12-13	8.435637804805275
2207	14-15	7.999801635234146
2207	16-17	7.269912846329838
2207	18-19	4.038234147974098
2207	20-21	7.4225247931258025
2207	22-23	6.224651672140414
2207	24-25	4.0853515688311965
2207	26-27	3.5286272970640162
2207	28-29	4.9850282218679105
2207	30-31	5.47474843660488
2207	32-33	6.553825045592028
2207	34-35	6.178567415324579
2207	36-37	6.9065720163572095
2207	38-39	6.800077632600036
2207	40-41	4.149312230034081
2207	42-43	5.743835667888295
2207	44-45	3.9644761104678423
2207	46-47	5.549253749048212
2207	48-49	2.384620085929569
2207	50-51	5.418750291001636
2207	52-53	2.72833510374727
2207	54-55	3.153991769088176
2207	56-57	5.218037499892404
2207	58-59	3.7862954961054847
2207	60-61	3.544102529861643
2207	62-63	4.4197059802340135
2207	64-65	3.5099712381955577
2207	66-67	3.6182139484517464
2207	68-69	3.4264055130285946
2207	70-71	3.4284970346503485
2207	72-73	3.82497236140134
2207	74-75	3.4219053632870953
2207	76-77	4.069818364564462
2207	78-79	4.200634515031798
2207	80-81	4.198413047753785
2207	82-83	3.8020945497927947
2207	84-85	3.181755944339905
2207	86-87	3.3053182605409503
2207	88-89	3.4400928790355643
2207	90-91	1.369071661938861
2207	92-93	-0.6119576396639772
2207	94-95	1.3911317986148681
2207	96-97	-1.215698856035221
2207	98-99	0.4120821337114151
2207	100	1.814575703865227
2208	1	1.124943085896188
2208	2	8.711162571025973
2208	3	6.746408053164295
2208	4	11.882523719056483
2208	5	10.097054789282005
2208	6	9.18107402728511
2208	7	7.602221066130429
2208	8	7.782761096519227
2208	9	6.752827777169159
2208	10-11	6.86931161298838
2208	12-13	7.091261831790224
2208	14-15	7.849261071317866
2208	16-17	7.108717407247781
2208	18-19	6.395140392568461
2208	20-21	6.641286685531959
2208	22-23	5.543941875680748
2208	24-25	6.150197616227839
2208	26-27	8.152357332236708
2208	28-29	7.2411351526180745
2208	30-31	6.150781274060915
2208	32-33	8.234674667918043
2208	34-35	6.701628592744868
2208	36-37	7.120555323743481
2208	38-39	7.3313777248562815
2208	40-41	7.448983567172981
2208	42-43	7.0873661989382875
2208	44-45	5.899412396000908
2208	46-47	4.792332368664198
2208	48-49	4.49971839648714
2208	50-51	5.999831995435699
2208	52-53	5.581899942587146
2208	54-55	6.523661088121791
2208	56-57	6.626925091427893
2208	58-59	6.0021967242667
2208	60-61	5.33142810790466
2208	62-63	3.4818333515077278
2208	64-65	3.3202210508000682
2208	66-67	3.1279765327797193
2208	68-69	5.574299476010779
2208	70-71	5.393950675888888
2208	72-73	4.624933440799371
2208	74-75	2.1117860067743806
2208	76-77	3.142924228601828
2208	78-79	3.9688918811160967
2208	80-81	3.489240038470502
2208	82-83	1.4890092992594397
2208	84-85	2.2869071580860876
2208	86-87	1.2100290948833354
2208	88-89	0.5759286629253175
2208	90-91	1.7676762862829776
2208	92-93	0.9173863319672257
2208	94-95	-0.8409769772601265
2208	96-97	1.2545074231552285
2208	98-99	0.2257252462063697
2208	100	1.7657908915490168
2209	1	5.186399540998963
2209	2	9.686888319313027
2209	3	5.800021017462889
2209	4	-2.855575812137168
2209	5	0.6651151030216376
2209	6	4.578319789996975
2209	7	2.183813655383947
2209	8	5.308371897096215
2209	9	5.183667571615608
2209	10-11	5.230174171589173
2209	12-13	7.314067682854052
2209	14-15	6.702976587004851
2209	16-17	6.276184951423765
2209	18-19	5.130921811609209
2209	20-21	5.933499541644473
2209	22-23	5.415502459885584
2209	24-25	5.22635723577346
2209	26-27	6.7683418255652334
2209	28-29	6.2237340978037885
2209	30-31	4.0313539841222195
2209	32-33	6.421135630774156
2209	34-35	5.661675247991891
2209	36-37	6.1185031690373854
2209	38-39	6.83177102633482
2209	40-41	7.139028193931008
2209	42-43	6.747248321188561
2209	44-45	6.31893660263632
2209	46-47	6.649979772198275
2209	48-49	5.036103854474881
2209	50-51	4.878558780506019
2209	52-53	5.027573626323171
2209	54-55	5.319965857324821
2209	56-57	4.9161605764621505
2209	58-59	3.1539930639709937
2209	60-61	4.0539905402883605
2209	62-63	4.162288859982301
2209	64-65	4.980894283760758
2209	66-67	4.444881734727069
2209	68-69	3.792382328517089
2209	70-71	3.4942245219040373
2209	72-73	0.49670138165764754
2209	74-75	2.1845862231465425
2209	76-77	3.1602599209927362
2209	78-79	3.476246280683352
2209	80-81	3.466821854193544
2209	82-83	2.8521730659381284
2209	84-85	2.795367084158933
2209	86-87	1.2066246141765227
2209	88-89	1.7665299972203066
2209	90-91	2.210040152132237
2209	92-93	2.164701964855798
2209	94-95	0.7046809293392222
2209	96-97	0.18613945525043718
2209	98-99	0.1422033385251602
2209	100	-0.018925570766164412
2210	1	1.62899074436681
2210	2	-17.017560833229346
2210	3	-7.533163241368916
2210	4	-7.701961751595059
2210	5	-1.2735905455967078
2210	6	2.429452756208587
2210	7	-8.245689218937319
2210	8	-8.140016037349472
2210	9	-3.400530668618295
2210	10-11	-1.4654222225940323
2210	12-13	3.063844758081032
2210	14-15	-4.957518601392341
2210	16-17	-1.4541399622916416
2210	18-19	-0.5529382287302163
2210	20-21	2.383271052339367
2210	22-23	2.53930848991482
2210	24-25	1.967658360770713
2210	26-27	4.1255408187284175
2210	28-29	3.473656125656678
2210	30-31	2.759066659164535
2210	32-33	4.84452026771935
2210	34-35	2.3742118639869645
2210	36-37	4.086960172378852
2210	38-39	3.0299037479818196
2210	40-41	3.894359840043254
2210	42-43	2.0098032108322066
2210	44-45	3.0626861217508328
2210	46-47	2.641104687012845
2210	48-49	2.8883239653808133
2210	50-51	2.1054395236527164
2210	52-53	2.150723934639469
2210	54-55	2.499657307455621
2210	56-57	2.743260259902101
2210	58-59	1.8598553328711667
2210	60-61	2.525422850846958
2210	62-63	1.5820603294584394
2210	64-65	2.6551221877595275
2210	66-67	2.758557499747184
2210	68-69	3.028233990457341
2210	70-71	2.3397067401326197
2210	72-73	0.6544826584498367
2210	74-75	0.17590555309444156
2210	76-77	-0.5146364138354613
2210	78-79	0.02221824643331516
2210	80-81	0.6752022456068509
2210	82-83	0.5281809332247214
2210	84-85	-1.0117837517574415
2210	86-87	-1.0174718019900233
2210	88-89	-1.7096661710841197
2210	90-91	-1.7906770542253483
2210	92-93	-1.331801997640632
2210	94-95	-0.5772220444566365
2210	96-97	-0.09531354712376583
2210	98-99	-0.41402797563870664
2210	100	-0.15459641976262262
2211	1	1.0706954756587521
2211	2	-17.017560833229346
2211	3	-7.941512082512547
2211	4	-18.808965642645642
2211	5	-21.090711168164802
2211	6	-14.760954958222136
2211	7	-13.118377145402102
2211	8	-17.231060981803317
2211	9	-16.934922762454647
2211	10-11	-11.279171658673661
2211	12-13	-22.076557317145948
2211	14-15	-17.58692070481534
2211	16-17	-14.604449413597116
2211	18-19	-18.983995438452204
2211	20-21	-9.622022750175718
2211	22-23	-5.148647933138246
2211	24-25	-1.800045214484463
2211	26-27	-17.111624705999493
2211	28-29	-3.6648797255811267
2211	30-31	-6.244204079007236
2211	32-33	-1.892331576300272
2211	34-35	-8.594661493594941
2211	36-37	-9.512303911532499
2211	38-39	-3.9539734160626168
2211	40-41	-8.405647561411662
2211	42-43	-2.9838949789833897
2211	44-45	-0.41397991050541094
2211	46-47	0.6740217766197958
2211	48-49	0.9634980757964229
2211	50-51	-6.667295278693192
2211	52-53	-1.3953606957107247
2211	54-55	-0.2658046843111421
2211	56-57	-9.543754063675408
2211	58-59	-3.5302982649408214
2211	60-61	-1.6143994437450466
2211	62-63	-0.8115543296516954
2211	64-65	-4.980098869027255
2211	66-67	-3.7893704453269734
2211	68-69	-0.03785747742739787
2211	70-71	-0.13480934569606262
2211	72-73	-0.9648252702706266
2211	74-75	-1.509446262729167
2211	76-77	-1.305254541055902
2211	78-79	-0.8117741688376476
2211	80-81	-0.3829731900216409
2211	82-83	-1.1326932138457124
2211	84-85	-1.223343766983291
2211	86-87	-1.742431975113579
2211	88-89	-2.4317397767909945
2211	90-91	-2.333998474740767
2211	92-93	-1.8216496686494885
2211	94-95	-1.751399425610419
2211	96-97	-2.747180828463641
2211	98-99	-2.503108010037849
2211	100	-1.9472217510658432
2212	1	-21.121333509848494
2212	2	-17.017560833229346
2212	3	-19.85093237236762
2212	4	-18.808965642645642
2212	5	-21.090711168164802
2212	6	-21.81892597271489
2212	7	-22.141927870039783
2212	8	-22.673089967310563
2212	9	-23.94398073346914
2212	10-11	-11.156597297540022
2212	12-13	-22.076557317145948
2212	14-15	-23.48637722655447
2212	16-17	-23.931442167220304
2212	18-19	-18.518102533039773
2212	20-21	-23.58397927191485
2212	22-23	-18.676655517402068
2212	24-25	-25.1886321710062
2212	26-27	-17.302059774512582
2212	28-29	-18.04377777207438
2212	30-31	-5.999984546019064
2212	32-33	-17.221083291726636
2212	34-35	-13.488210840693274
2212	36-37	-17.534126214154504
2212	38-39	-23.575803126207546
2212	40-41	-10.031366870874578
2212	42-43	-13.752099291461297
2212	44-45	-16.929944421972777
2212	46-47	-16.978005935313337
2212	48-49	-17.617850821132443
2212	50-51	-9.02532750271748
2212	52-53	-1.9110081132143115
2212	54-55	-23.360913379963314
2212	56-57	-23.176000440487
2212	58-59	-12.376162657887821
2212	60-61	-6.281929671636842
2212	62-63	-3.0193502926816578
2212	64-65	-17.086218782528178
2212	66-67	-17.408615319840933
2212	68-69	-14.328305151725111
2212	70-71	-9.497375753489294
2212	72-73	-17.143033377479576
2212	74-75	-6.1126713942451865
2212	76-77	-10.742807173455624
2212	78-79	-4.652625488156467
2212	80-81	-2.242269241422047
2212	82-83	-2.7837176612704937
2212	84-85	-4.944850145250399
2212	86-87	-4.667406113761615
2212	88-89	-2.8808221536441714
2212	90-91	-4.222543195386187
2212	92-93	-5.4307046506635
2212	94-95	-3.9905675477903593
2212	96-97	-5.032054666652151
2212	98-99	-3.5561946078795295
2212	100	-3.401110570644576
2213	1	-21.121333509848494
2213	2	-17.017560833229346
2213	3	-19.85093237236762
2213	4	-18.808965642645642
2213	5	-21.090711168164802
2213	6	-21.81892597271489
2213	7	-10.712080931264273
2213	8	-10.532783844861584
2213	9	-4.440154202856895
2213	10-11	-17.579369455183567
2213	12-13	-16.1320420110235
2213	14-15	-23.48637722655447
2213	16-17	-18.02136563660806
2213	18-19	-18.624985530141053
2213	20-21	-17.643290496404646
2213	22-23	-19.746076578509438
2213	24-25	-7.723708701618445
2213	26-27	-23.225302242231376
2213	28-29	-24.115513783552142
2213	30-31	-19.465819355552636
2213	32-33	-23.260538098039405
2213	34-35	-17.8045291363042
2213	36-37	-23.61375318689482
2213	38-39	-11.654884758860607
2213	40-41	-17.09015288528034
2213	42-43	-23.117952949997882
2213	44-45	-17.069277899266133
2213	46-47	-16.83221713198713
2213	48-49	-6.171604334733004
2213	50-51	-23.183146727968555
2213	52-53	-17.7652586548944
2213	54-55	-17.64917868608576
2213	56-57	-17.375617787425778
2213	58-59	-17.818637513683797
2213	60-61	-19.05200111484827
2213	62-63	-17.34775848522697
2213	64-65	-17.436916378639793
2213	66-67	-8.865559072951935
2213	68-69	-22.45814733249986
2213	70-71	-17.089380848210112
2213	72-73	-9.42035802685152
2213	74-75	-4.633383337559099
2213	76-77	-3.1574339997959164
2213	78-79	-4.842663698562582
2213	80-81	-3.429640226401407
2213	82-83	-3.253959111509122
2213	84-85	-3.1744584519877286
2213	86-87	-3.5902395764149695
2213	88-89	-3.007845218967862
2213	90-91	-3.1029462712506763
2213	92-93	-3.7830885920449298
2213	94-95	-3.781272244303114
2213	96-97	-3.3280370840093383
2213	98-99	-4.067715374722852
2213	100	-3.452804418915594
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	272.0
3	276.0
4	1524.0
5	5022.0
6	7382.0
7	9265.0
8	9123.0
9	9551.0
10	10353.0
11	11826.0
12	14660.0
13	18381.0
14	15511.0
15	17243.0
16	19273.0
17	19901.0
18	19621.0
19	19487.0
20	19129.0
21	18851.0
22	19785.0
23	21096.0
24	22744.0
25	24880.0
26	27104.0
27	29051.0
28	30719.0
29	32179.0
30	34102.0
31	36762.0
32	41703.0
33	49449.0
34	57641.0
35	65005.0
36	67361.0
37	54449.0
38	16408.0
39	78.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.77838642796437	20.343387343028837	11.895462032345648	24.98276419666114
2	15.849956466005324	22.635340881302497	37.27460060090931	24.24010205178287
3	18.76901150011775	27.827562014286833	28.91452920166418	24.488897283931234
4	13.307428620751308	17.616540379908383	35.72075932353866	33.35527167580165
5	13.54526258230375	37.904252255265384	34.37108057638173	14.179404586049142
6	31.424844646917787	36.64736073590878	17.84764195524698	14.080152661926457
7	26.071747067817423	32.593979728399184	23.00491801428118	18.329355189502216
8	25.943173108784567	34.14811517145957	21.99392423249802	17.914787487257836
9	25.990180948996432	16.23879717842243	21.177653261414587	36.593368611166554
10-11	24.0615378524356	27.729583835704403	26.88089892923048	21.32797938262952
12-13	24.68825119950442	25.742771903230853	27.384568426326183	22.18440847093854
14-15	23.09900545316146	26.276251918589416	25.002058974007014	25.622683654242113
16-17	21.838700888098128	29.955639040155894	26.739626403623205	21.466033668122762
18-19	21.994409865233425	28.6260458415677	28.255976457406007	21.12356783579287
20-21	23.40738233456626	28.150742115319645	26.717095233990772	21.724780316123322
22-23	21.784106024919762	28.47284087719512	26.79679777406246	22.946255323822655
24-25	22.330472416056796	28.158476987040775	26.08810085341243	23.422949743489994
26-27	22.65870147812238	27.641016456983504	26.614133945646547	23.08614811924757
28-29	22.106179660675355	27.456945390016884	27.673908181297573	22.762966768010184
30-31	23.32859285741671	28.470591477207375	26.33396273434804	21.86685293102787
32-33	21.15464351513138	28.28643304647509	27.65586901309692	22.903054425296613
34-35	21.228086331794746	27.516921763522166	27.814724889894975	23.44026701478811
36-37	22.916287751581514	28.203200702190433	26.67671865019532	22.203792896032738
38-39	22.96275221673578	27.195719635119254	27.042306374008284	22.799221774136683
40-41	22.467017534084203	27.988303856586743	26.64532420200035	22.899354407328705
42-43	22.59442659546259	27.803321349506362	27.412541701867877	22.189710353163175
44-45	23.283914883436136	26.794735741914955	26.88716795469527	23.034181419953644
46-47	22.221733292507704	27.877611113861338	28.017872825714278	21.882782767916677
48-49	22.24104968898856	27.516661113957124	28.100412094637278	22.14187710241704
50-51	22.164044243459752	27.639658972062662	27.41700039789056	22.77929638658702
52-53	21.113388344488833	27.877133048213803	27.252758642924245	23.756719964373115
54-55	22.13974797319297	27.732043711486725	26.513867658944662	23.614340656375642
56-57	22.470340928310822	27.305071640853406	26.595851354289994	23.628736076545774
58-59	21.953843412142092	26.44683378876084	27.81691814185577	23.782404657241297
60-61	22.877202143592427	27.391106211303985	26.655017315335527	23.076674329768064
62-63	21.219055654621577	27.68609739351978	27.633038971351997	23.461807980506645
64-65	21.234734965011835	26.8866696136717	27.66329565376901	24.21529976754746
66-67	22.303621704557365	27.1484184112637	26.921333284662424	23.62662659951651
68-69	23.173051427780916	26.63742926017995	26.704172094578237	23.485347217460898
70-71	22.61310640980925	27.20194378161042	26.41244424891086	23.772505559669472
72-73	22.71303780260697	27.31428385606451	27.03067284447792	22.942005496850605
74-75	23.228922185521053	26.38982401677183	26.557715069013877	23.823538728693244
76-77	22.627736383731467	27.3921750823873	27.314606936115936	22.6654815977653
78-79	22.84436940252266	27.015557299724275	27.33038040009472	22.809692897658344
80-81	22.761514266917633	27.002485660439916	26.873252407306193	23.362747665336254
82-83	21.9581390027933	27.40746148791706	27.032012872523957	23.60238663676568
84-85	22.44905979665531	26.959238590180455	26.25229444755251	24.33940716561172
86-87	23.06248190166548	26.803081519199285	26.466775041644087	23.667661537491146
88-89	22.7912368545645	26.01655643391056	27.303080300139165	23.889126411385778
90-91	23.63507426140036	26.494385322694658	26.412898071792213	23.457642344112774
92-93	22.54388749245784	26.74330356504557	26.9811639085962	23.73164503390039
94-95	22.44752601978817	26.34741184182079	26.869441996044202	24.335620142346837
96-97	23.41195448402145	26.453179532954106	26.449852341539643	23.6850136414848
98-99	23.510076229790396	26.059967587874493	26.332818336853524	24.09713784548158
100	23.294346085857427	26.218275157765547	26.161259742995576	24.32611901338145
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	330.0
1	372.5
2	357.0
3	282.5
4	238.0
5	229.5
6	276.5
7	369.0
8	508.5
9	707.5
10	922.5
11	1169.5
12	1495.5
13	1806.0
14	2115.0
15	2444.5
16	2775.0
17	3111.5
18	3393.0
19	3627.0
20	3923.5
21	4237.5
22	4627.5
23	5100.0
24	5607.0
25	6295.0
26	7246.0
27	8282.5
28	9635.0
29	11356.0
30	12880.0
31	14577.0
32	16590.0
33	18429.5
34	20211.0
35	22132.5
36	23768.5
37	25400.0
38	26911.0
39	27924.0
40	28467.0
41	28650.5
42	28654.0
43	28330.0
44	27865.0
45	27458.0
46	27352.5
47	28242.5
48	29919.5
49	32851.5
50	36521.0
51	35092.0
52	28824.5
53	23805.5
54	20864.5
55	18917.0
56	17283.0
57	15476.0
58	14071.0
59	12651.5
60	10847.0
61	9344.5
62	7975.5
63	6503.0
64	5356.0
65	4514.0
66	3813.0
67	3263.0
68	2732.0
69	2169.0
70	1674.0
71	1350.5
72	1110.5
73	927.0
74	746.5
75	596.0
76	475.0
77	349.0
78	236.0
79	150.0
80	92.5
81	56.0
82	34.0
83	17.5
84	12.5
85	7.0
86	3.5
87	3.5
88	4.5
89	3.0
90	1.5
91	1.0
92	0.5
93	0.5
94	0.5
95	0.5
96	0.5
97	0.0
98	0.0
99	0.0
100	0.5
>>END_MODULE
>>Per base N content	fail
#Base	N-Count
1	9.052894146724626
2	26.806754016053958
3	7.053502924756631
4	26.03597718564424
5	14.10312973470274
6	11.462925531854253
7	9.108185784462936
8	9.85958204082005
9	6.327073407914343
10-11	7.8567137158602645
12-13	11.480197043436426
14-15	8.641113949795193
16-17	9.205316661479513
18-19	7.412727564990475
20-21	9.399065400317157
22-23	6.582212965148028
24-25	6.223102328290963
26-27	10.87957025287089
28-29	7.231576199287022
30-31	4.84639755029544
32-33	10.09021087204603
34-35	8.392472585037968
36-37	8.1726170729177
38-39	7.036573423304798
40-41	9.97586548513567
42-43	7.564352056107902
44-45	7.558708888957291
46-47	6.732298410678925
48-49	4.530266186484444
50-51	7.454224794138402
52-53	5.41020125016103
54-55	5.988882390696412
56-57	5.6348449041060595
58-59	4.043357764256977
60-61	4.812481545703384
62-63	3.526124443806025
64-65	5.223007705488236
66-67	3.1836582999588448
68-69	6.054548335721703
70-71	3.0862424145003176
72-73	1.8387034623965564
74-75	1.031103541286893
76-77	1.2348845772811792
78-79	2.02874709148885
80-81	2.6986309334482486
82-83	0.8444800134979998
84-85	1.3720306395475435
86-87	0.592190540683815
88-89	0.6731329381976294
90-91	0.24892637319917418
92-93	0.33226284162536895
94-95	0.6022228378404568
96-97	0.6339727782736925
98-99	0.4954016737975779
100	0.4239785582448952
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	877167.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	91.50980037041951
#Duplication Level	Percentage of deduplicated	Percentage of total
1	97.10765738235588	88.86302341498482
2	2.0595561444754145	3.7693914326523212
3	0.39490392889103415	1.0841273909493863
4	0.12962908392675915	0.4744932636935235
5	0.07333982528393765	0.33556563854672905
6	0.04319034959561071	0.23714041616537795
7	0.03383372408779874	0.21672821359436223
8	0.02167474029856617	0.15867609262419818
9	0.01426935115216514	0.11752069278150429
>10	0.10968720577566574	1.8932209744560817
>50	0.006255600108911876	0.3782509897044752
>100	0.004752109152729099	0.9866847415417345
>500	7.503329373136631E-4	0.502154070556676
>1k	5.002219582091088E-4	0.983022667748826
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT	3384	0.3857874270235884	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA	2642	0.3011969214528134	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC	1317	0.15014244721928663	No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG	1247	0.14216221084468522	No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC	961	0.10955724508559944	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	1.1400337678002023E-4	0.0
9	0.0	0.0	0.0	1.1400337678002023E-4	0.0
10-11	0.0	0.0	0.0	1.1400337678002023E-4	0.0
12-13	0.0	0.0	0.0	1.1400337678002023E-4	0.0
14-15	0.0	0.0	0.0	1.1400337678002023E-4	0.0
16-17	0.0	0.0	0.0	1.7100506517003035E-4	0.0
18-19	0.0	0.0	0.0	2.2800675356004046E-4	0.0
20-21	0.0	0.0	0.0	2.2800675356004046E-4	0.0
22-23	0.0	0.0	0.0	3.420101303400607E-4	0.0
24-25	0.0	0.0	0.0	4.560135071200809E-4	0.0
26-27	0.0	0.0	0.0	0.0011400337678002023	0.0
28-29	0.0	0.0	0.0	0.008151241439771445	0.0
30-31	0.0	0.0	0.0	0.031635937056455614	0.0
32-33	0.0	0.0	0.0	0.0806003873834743	0.0
34-35	0.0	0.0	0.0	0.1679839756853598	0.0
36-37	0.0	0.0	0.0	0.29008159221676144	0.0
38-39	0.0	0.0	0.0	0.4775031436431147	0.0
40-41	0.0	0.0	0.0	0.7539613323346638	0.0
42-43	0.0	0.0	0.0	0.9652665911964313	0.0
44-45	0.0	0.0	0.0	1.1973204646321625	0.0
46-47	1.1400337678002023E-4	0.0	0.0	1.4888270990586743	0.0
48-49	1.1400337678002023E-4	0.0	0.0	1.7688763941187937	0.0
50-51	1.7100506517003035E-4	0.0	0.0	2.0712133493394074	0.0
52-53	2.2800675356004046E-4	0.0	0.0	2.3935008954965244	0.0
54-55	2.2800675356004046E-4	0.0	0.0	2.7307228840118247	0.0
56-57	2.8500844195005056E-4	0.0	0.0	3.10129086023528	0.0
58-59	3.420101303400607E-4	0.0	0.0	3.4922084392139694	0.0
60-61	3.9901181873007083E-4	0.0	0.0	3.88962421066912	0.0
62-63	4.560135071200809E-4	0.0	0.0	4.275525641069488	0.0
64-65	5.700168839001011E-4	0.0	0.0	4.7211648409025875	0.0
66-67	5.700168839001011E-4	0.0	0.0	5.248259453445011	0.0
68-69	6.840202606801214E-4	0.0	0.0	5.824660526444793	0.0
70-71	7.980236374601416E-4	0.0	0.0	6.53735263638509	0.0
72-73	7.980236374601416E-4	0.0	0.0	7.190762990399776	0.0
74-75	7.980236374601416E-4	0.0	0.0	7.824393758543128	0.0
76-77	7.980236374601416E-4	0.0	0.0	8.526312549377714	0.0
78-79	7.980236374601416E-4	0.0	0.0	9.266308468056824	0.0
80-81	8.550253258501517E-4	0.0	0.0	10.101440204658862	0.0
82-83	9.120270142401618E-4	0.0	0.0	10.99146456718048	0.0
84-85	9.120270142401618E-4	0.0	0.0	11.863248389417294	0.0
86-87	9.120270142401618E-4	0.0	0.0	12.771513292223716	0.0
88	9.120270142401618E-4	0.0	0.0	13.546109235755564	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GACGCGA	115	2.4062447E-6	30.722948	6
ACGCGAT	105	4.7554975E-5	28.668602	7
TCTACGG	80	0.006696458	28.425375	3
TACACCG	75	0.008740992	26.915415	5
CTGTGCG	140	1.1026708E-5	25.261288	9
GTGTATA	225	1.0934673E-7	25.211302	1
GTACATA	350	2.910383E-11	23.410492	1
AGTACGT	135	0.001830849	23.335693	2
CCGCAGA	230	3.7217706E-6	21.92287	1
GCGATGT	145	4.052683E-4	20.905893	9
TACATAG	340	1.26165105E-8	20.384417	2
GTTGACG	140	0.0041068057	20.30384	3
CATGGGT	705	0.0	20.15984	4
TCTAGGC	230	9.091396E-6	19.774176	3
GTACAAG	255	9.091267E-6	19.773567	1
CATGGGG	1545	0.0	19.134233	4
GATATAT	330	2.0514926E-7	19.09947	1
GTATTAG	345	3.2733624E-7	18.269058	1
GAGTACG	175	0.008212854	18.008072	1
GTGTAAG	280	2.0358902E-5	18.008072	1
>>END_MODULE