FastQCFastQC Report
Wed 25 May 2016
SRR1295093_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295093_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences877167
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT105621.2041036655505737No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA64360.7337257329562101No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC36830.4198744366808145No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG29440.33562594124037953No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC24500.27930827311104955No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC23670.26984599283830785No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG22480.25627959100148545No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG16810.191639676367214No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG15010.17111906854681036No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT12350.14079417032332497No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11480.13087587654346322No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT10860.12380766718310197No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT10650.12141359627072154No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA10190.1161694409388406No Hit
CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTC9810.11183731262119984No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC9590.10932923833203939No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCCG650.006153141628.9233065
TAGCACG700.00883455426.8573534
CTGTCGC1901.3505996E-824.7045889
CATGGGT9700.023.7424564
ATATACG1100.003079150821.3394326
GATCCGT1150.003983968420.411636
ATGGGGG8750.020.411595
TCTGTCG1857.254732E-620.2989838
CATGGGG17300.019.8325274
CGACGGT2251.8664687E-618.7765587
TATACGC1250.006456322518.7765567
CTATACT2302.2754866E-618.3914494
TACGACG2051.7401422E-518.3416085
GTCTCGC1650.001008833118.2577651
GCCTAGA1951.4882079E-418.0236911
AGCACCG1350.00998853117.4075455
CTAGCAC2705.440779E-717.4075453
TATTCGG1750.001503558717.2029192
CTGGACG1650.001570811517.0910454
AGTACTT23900.017.0864812-13
Produced by FastQC (version 0.11.5)