##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1295093_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 877167 Sequences flagged as poor quality 0 Sequence length 100 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.969171206851147 33.0 33.0 33.0 27.0 33.0 2 29.4357186259857 33.0 33.0 33.0 15.0 33.0 3 30.4144262153045 33.0 33.0 33.0 22.0 33.0 4 34.723855320594595 37.0 33.0 37.0 27.0 37.0 5 35.11669613653957 37.0 37.0 37.0 33.0 37.0 6 34.78261608108832 37.0 37.0 37.0 27.0 37.0 7 34.64155856296463 37.0 37.0 37.0 27.0 37.0 8 34.649728044944695 37.0 37.0 37.0 27.0 37.0 9 34.605777463128454 37.0 37.0 37.0 27.0 37.0 10-11 34.5756001992779 37.0 37.0 37.0 27.0 37.0 12-13 34.4537220392468 37.0 37.0 37.0 27.0 37.0 14-15 35.71697293673839 37.0 37.0 40.0 27.0 40.0 16-17 35.68368509075239 37.0 37.0 40.0 27.0 40.0 18-19 35.731506087210306 37.0 37.0 40.0 27.0 40.0 20-21 35.74254845428521 37.0 37.0 40.0 27.0 40.0 22-23 35.72267823572934 37.0 37.0 40.0 27.0 40.0 24-25 35.67653251889321 37.0 37.0 40.0 27.0 40.0 26-27 35.614828191211025 37.0 37.0 40.0 27.0 40.0 28-29 35.54481529742911 37.0 37.0 40.0 27.0 40.0 30-31 35.423031760200736 37.0 33.0 40.0 27.0 40.0 32-33 35.33516707764884 37.0 33.0 40.0 27.0 40.0 34-35 35.20095660233456 37.0 33.0 40.0 27.0 40.0 36-37 35.16794863463856 37.0 33.0 40.0 27.0 40.0 38-39 35.076987620373316 37.0 33.0 40.0 27.0 40.0 40-41 34.96973096343113 37.0 33.0 40.0 27.0 40.0 42-43 34.80258833266642 37.0 33.0 40.0 22.0 40.0 44-45 34.629666300715826 37.0 33.0 40.0 22.0 40.0 46-47 34.447697530800866 37.0 33.0 40.0 22.0 40.0 48-49 34.30604605508415 37.0 33.0 40.0 22.0 40.0 50-51 34.19586920164575 37.0 33.0 40.0 22.0 40.0 52-53 34.10805297052899 37.0 33.0 40.0 22.0 40.0 54-55 33.9751598042334 37.0 33.0 40.0 22.0 40.0 56-57 33.801176400844994 37.0 33.0 40.0 22.0 40.0 58-59 33.68325472800504 37.0 33.0 40.0 22.0 40.0 60-61 33.496512066687416 37.0 33.0 40.0 22.0 40.0 62-63 33.27880665825322 37.0 33.0 40.0 22.0 40.0 64-65 33.118478009318636 37.0 33.0 37.0 22.0 40.0 66-67 32.87751819208885 37.0 33.0 37.0 22.0 40.0 68-69 32.655776494099754 37.0 33.0 37.0 22.0 40.0 70-71 32.396808133456915 37.0 33.0 37.0 22.0 40.0 72-73 32.16823706318181 37.0 33.0 37.0 22.0 40.0 74-75 31.95352538342186 37.0 33.0 37.0 22.0 37.0 76-77 31.705771534952866 33.0 27.0 37.0 15.0 37.0 78-79 31.481496681931716 33.0 27.0 37.0 15.0 37.0 80-81 31.31463506949076 33.0 27.0 37.0 15.0 37.0 82-83 31.135396680449674 33.0 27.0 37.0 15.0 37.0 84-85 30.955527852734996 33.0 27.0 37.0 15.0 37.0 86-87 30.993427135311748 33.0 27.0 37.0 15.0 37.0 88-89 31.036562592984005 33.0 27.0 37.0 15.0 37.0 90-91 31.067714015689145 33.0 27.0 37.0 15.0 37.0 92-93 31.036941654211795 33.0 27.0 37.0 15.0 37.0 94-95 31.111265015669765 33.0 27.0 37.0 15.0 37.0 96-97 31.073387393734603 33.0 27.0 37.0 15.0 37.0 98-99 30.993849517822717 33.0 27.0 37.0 15.0 37.0 100 30.381433638064358 33.0 27.0 37.0 15.0 37.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.8768517513480383 1101 2 4.1485713584788435 1101 3 2.3844608045785662 1101 4 1.310622666594476 1101 5 0.6515930756876287 1101 6 0.8068406633381002 1101 7 0.7941941309660265 1101 8 0.7036125142952017 1101 9 0.7884767199232599 1101 10-11 0.789809713884317 1101 12-13 0.8119082224450551 1101 14-15 1.183488355514207 1101 16-17 1.1426445619758354 1101 18-19 1.178228824389734 1101 20-21 1.1813923257336398 1101 22-23 1.0541801903788155 1101 24-25 1.1529814043007605 1101 26-27 1.1493313224232864 1101 28-29 0.9756524741002792 1101 30-31 0.8352732699834533 1101 32-33 1.0218076702827972 1101 34-35 1.2697754007621214 1101 36-37 1.1658383768722018 1101 38-39 1.1600226605527837 1101 40-41 1.1989192386379912 1101 42-43 1.288351102494076 1101 44-45 1.5204414870182106 1101 46-47 1.3017903818277432 1101 48-49 1.1246736944422722 1101 50-51 1.2259695248313918 1101 52-53 1.1197802466840514 1101 54-55 1.1399446450411972 1101 56-57 1.2697591576254936 1101 58-59 1.2248194406290125 1101 60-61 1.047587266777228 1101 62-63 1.3306647727691399 1101 64-65 1.3019886500459563 1101 66-67 1.153438499832106 1101 68-69 1.1980366650475034 1101 70-71 1.1531506437431744 1101 72-73 1.2763402912017732 1101 74-75 1.3330388020904884 1101 76-77 1.3409311341741805 1101 78-79 1.3266098163724678 1101 80-81 1.2203046554008417 1101 82-83 1.1250215119951719 1101 84-85 1.1122417691020772 1101 86-87 1.1833388735971049 1101 88-89 1.107039857000533 1101 90-91 1.0994881667604481 1101 92-93 1.239365231132826 1101 94-95 1.2138107542350198 1101 96-97 1.2546560866726182 1101 98-99 1.1837981089655294 1101 100 1.4337700612017166 1102 1 0.5829637091386211 1102 2 4.074954154621075 1102 3 2.3481144182271407 1102 4 1.0830991114053887 1102 5 0.6971753775646334 1102 6 0.8854691352386368 1102 7 0.6748437863237271 1102 8 0.6233161288702362 1102 9 0.7400067649491078 1102 10-11 0.9324897130505079 1102 12-13 0.8861659850031884 1102 14-15 1.2490130954111933 1102 16-17 1.283142029271879 1102 18-19 1.1099300672328454 1102 20-21 1.0810404454875737 1102 22-23 1.130778561666034 1102 24-25 1.198689646532408 1102 26-27 1.1378782776197909 1102 28-29 1.1507496653118992 1102 30-31 0.9560903131007947 1102 32-33 1.0750894153586756 1102 34-35 1.2047347350558866 1102 36-37 1.0331952449353423 1102 38-39 1.143076010624128 1102 40-41 1.055683654321065 1102 42-43 1.0508210794715893 1102 44-45 1.274900499367888 1102 46-47 1.1898496728096077 1102 48-49 1.1578113521717128 1102 50-51 1.0637034843729793 1102 52-53 1.1857143651131494 1102 54-55 1.0924506554360534 1102 56-57 0.9657185857231028 1102 58-59 1.015894235881838 1102 60-61 1.144617361600183 1102 62-63 1.2381294457758614 1102 64-65 1.0477149292525354 1102 66-67 0.9077578197194498 1102 68-69 0.9723958457808948 1102 70-71 1.0591771358034379 1102 72-73 1.0112458330406007 1102 74-75 0.8381702201267593 1102 76-77 0.889514001190431 1102 78-79 0.9785348508830225 1102 80-81 1.0024014957165726 1102 82-83 0.9925630925784681 1102 84-85 0.9816477012112514 1102 86-87 1.0521725732672884 1102 88-89 0.8363536470929951 1102 90-91 1.1397355194073349 1102 92-93 1.1475929289778826 1102 94-95 1.1106063973356903 1102 96-97 1.1220100595551976 1102 98-99 1.0942963521699625 1102 100 0.9153706096647234 1103 1 0.5759233620609372 1103 2 4.0875667181394775 1103 3 2.267865301859551 1103 4 1.2437121929152042 1103 5 0.570811841347421 1103 6 0.49009871278265393 1103 7 0.6400545689829684 1103 8 0.6961451940480856 1103 9 0.78389540382706 1103 10-11 0.6987721636889717 1103 12-13 0.7501456298157692 1103 14-15 1.1115477551306796 1103 16-17 1.1497998185970388 1103 18-19 1.2226796289324184 1103 20-21 1.1160233236447397 1103 22-23 1.0466940235924937 1103 24-25 0.9039176682271091 1103 26-27 0.9095650662605479 1103 28-29 0.9623374672001077 1103 30-31 0.9788514651512017 1103 32-33 1.0205678894169097 1103 34-35 0.9899776343545952 1103 36-37 1.065176673419714 1103 38-39 0.9192433663834407 1103 40-41 0.9705238738931641 1103 42-43 0.951067154690513 1103 44-45 1.0657454992272548 1103 46-47 0.8824578064467019 1103 48-49 0.9786244111805402 1103 50-51 1.027041089377569 1103 52-53 0.9362263504031247 1103 54-55 0.8738462672753187 1103 56-57 0.8121643091297557 1103 58-59 1.0009114514787782 1103 60-61 1.0551927949785238 1103 62-63 0.9177879406006397 1103 64-65 0.9285243142725079 1103 66-67 0.8936088608587482 1103 68-69 0.8696662537049846 1103 70-71 1.037856867104182 1103 72-73 0.8708659121221807 1103 74-75 0.9933461496986844 1103 76-77 0.7875581734354746 1103 78-79 0.8299162882740347 1103 80-81 0.9195518749519174 1103 82-83 0.9644405243254823 1103 84-85 0.9233437832273381 1103 86-87 1.0417094690220985 1103 88-89 0.9908180709271619 1103 90-91 0.9391705923480238 1103 92-93 1.0565216594535904 1103 94-95 0.9527949520693362 1103 96-97 0.9884024075797981 1103 98-99 0.7750394647592636 1103 100 0.8038313548223215 1104 1 0.32198100208269054 1104 2 3.5095351792596503 1104 3 2.05796861887967 1104 4 0.8184810890544085 1104 5 0.3359015640227909 1104 6 0.22745597216275115 1104 7 0.19905402520917903 1104 8 0.018603051579354712 1104 9 0.19444461535506008 1104 10-11 0.2400989717368205 1104 12-13 0.2512331773959744 1104 14-15 0.45140637394525385 1104 16-17 0.365949900163109 1104 18-19 0.3920651645496065 1104 20-21 0.4683887396316919 1104 22-23 0.41020680227656214 1104 24-25 0.3506278367969813 1104 26-27 0.3089669150914318 1104 28-29 0.5313967385432292 1104 30-31 0.51800317803864 1104 32-33 0.730464572396798 1104 34-35 0.652022223805389 1104 36-37 0.48116362284875436 1104 38-39 0.39232656377332376 1104 40-41 0.4552982077702765 1104 42-43 0.47281810629464616 1104 44-45 0.49614245409403424 1104 46-47 0.5363457890459387 1104 48-49 0.3560034215122485 1104 50-51 0.3054532699104726 1104 52-53 0.5322024243563632 1104 54-55 0.6057657887543897 1104 56-57 0.3912833955897881 1104 58-59 0.5599109077049818 1104 60-61 0.4358344480508407 1104 62-63 0.41479718475506644 1104 64-65 0.516615668269246 1104 66-67 0.5926300680365628 1104 68-69 0.7149626104205922 1104 70-71 0.5974000971204987 1104 72-73 0.6136609094033183 1104 74-75 0.4009046053811183 1104 76-77 0.5602607291722812 1104 78-79 0.5017488059466828 1104 80-81 0.658540455702326 1104 82-83 0.6267570006713221 1104 84-85 0.5299234460875866 1104 86-87 0.4171852710884387 1104 88-89 0.5528082829989422 1104 90-91 0.4413456875084414 1104 92-93 0.4105183968108541 1104 94-95 0.46774873129717776 1104 96-97 0.46121371807463163 1104 98-99 0.3620432711757964 1104 100 0.5504327686341774 1105 1 0.5446133790815466 1105 2 3.834346705998744 1105 3 2.23150118448838 1105 4 1.1243203453812072 1105 5 0.5270005248138503 1105 6 0.5949915554140617 1105 7 0.4827639024819348 1105 8 0.45569106960584094 1105 9 0.47423103964412405 1105 10-11 0.5040432037232065 1105 12-13 0.41109677672987743 1105 14-15 0.7090391204572342 1105 16-17 0.6129884034307835 1105 18-19 0.5973588929772973 1105 20-21 0.7001752651414677 1105 22-23 0.662853134506534 1105 24-25 0.7579486250878134 1105 26-27 0.6633668099599177 1105 28-29 0.6467882111004855 1105 30-31 0.6914770816414304 1105 32-33 0.6829152783394008 1105 34-35 0.879924979453115 1105 36-37 0.8055072913348056 1105 38-39 0.7733055233796478 1105 40-41 0.802077074150013 1105 42-43 0.8891624913773555 1105 44-45 0.8775776258198675 1105 46-47 1.0567199088642383 1105 48-49 0.9805589633608278 1105 50-51 1.0157604431523168 1105 52-53 0.727002698917552 1105 54-55 0.8329076636783626 1105 56-57 0.8767373748148088 1105 58-59 0.8338046759883326 1105 60-61 0.745087459295263 1105 62-63 0.7584666772625823 1105 64-65 0.723771915384674 1105 66-67 0.6972777667461969 1105 68-69 0.8615671586239415 1105 70-71 0.7967196466077979 1105 72-73 0.8385253182153853 1105 74-75 0.8007048670021568 1105 76-77 0.8599587348653763 1105 78-79 0.8018413423854156 1105 80-81 0.8869199839275197 1105 82-83 0.9558289993692561 1105 84-85 0.8842119119007208 1105 86-87 1.0073303704098322 1105 88-89 0.8272205198933769 1105 90-91 0.9465561101365623 1105 92-93 0.6962538386407147 1105 94-95 0.7028555550975035 1105 96-97 0.6058703426110519 1105 98-99 0.853650866065184 1105 100 0.8689346790314154 1106 1 0.5003698287808618 1106 2 3.907924023193125 1106 3 2.2134818904933837 1106 4 1.2237847363029246 1106 5 0.4478715677025278 1106 6 0.396180103051087 1106 7 0.4584789348746554 1106 8 0.30941947923546564 1106 9 0.2139101658635454 1106 10-11 0.2860008908015672 1106 12-13 0.3652291908705365 1106 14-15 0.5813071244564938 1106 16-17 0.4893507872641223 1106 18-19 0.5064274281997427 1106 20-21 0.5919680121123463 1106 22-23 0.4226080332218629 1106 24-25 0.4336782957299974 1106 26-27 0.4897665297100744 1106 28-29 0.3829559732613319 1106 30-31 0.3632642023843644 1106 32-33 0.5270892969551184 1106 34-35 0.729088154869352 1106 36-37 0.4722241023865763 1106 38-39 0.4276925424928919 1106 40-41 0.5640589981467699 1106 42-43 0.5762415544064723 1106 44-45 0.6029211846967755 1106 46-47 0.5496385160068655 1106 48-49 0.3191151347099108 1106 50-51 0.44211370601532707 1106 52-53 0.6558648571709966 1106 54-55 0.7213776118271156 1106 56-57 0.533951196339352 1106 58-59 0.6193240111883185 1106 60-61 0.5979505640065099 1106 62-63 0.7499841748594207 1106 64-65 0.539265440892521 1106 66-67 0.4201016750302955 1106 68-69 0.3041919252570011 1106 70-71 0.24684050994045492 1106 72-73 0.3662494363795794 1106 74-75 0.5026885453540082 1106 76-77 0.4723697355657208 1106 78-79 0.49141365687424354 1106 80-81 0.4981392523335124 1106 82-83 0.47921807258648386 1106 84-85 0.6236778895639858 1106 86-87 0.4407851397554374 1106 88-89 0.5328291204795192 1106 90-91 0.5093803201250431 1106 92-93 0.4276421006083915 1106 94-95 0.50361668819858 1106 96-97 0.4857826416179698 1106 98-99 0.5123441564671012 1106 100 0.35757145852713634 1107 1 0.5875845449071804 1107 2 3.5526079389577205 1107 3 2.174120774159345 1107 4 0.9410951663440201 1107 5 0.5297477036897078 1107 6 0.42577816504577015 1107 7 0.2729954520163034 1107 8 0.1182112925172234 1107 9 0.3527697690402789 1107 10-11 0.4251931759377072 1107 12-13 0.3425009134076191 1107 14-15 0.32421126168822667 1107 16-17 0.22107580338058597 1107 18-19 0.3188630742462877 1107 20-21 0.43402298493818137 1107 22-23 0.33124612449479685 1107 24-25 0.41923866757273487 1107 26-27 0.4243244348377928 1107 28-29 0.3697234014311306 1107 30-31 0.33194872923171914 1107 32-33 0.4216429659139038 1107 34-35 0.6290251719348987 1107 36-37 0.5701299687810462 1107 38-39 0.4557038598351113 1107 40-41 0.44010796758815474 1107 42-43 0.48703802131846885 1107 44-45 0.5648853401043041 1107 46-47 0.6555586880053994 1107 48-49 0.48673997578362815 1107 50-51 0.45848814977333063 1107 52-53 0.45780031631127116 1107 54-55 0.5077962135891809 1107 56-57 0.5268122444022438 1107 58-59 0.46689145972754176 1107 60-61 0.48054572951369323 1107 62-63 0.3306785764012332 1107 64-65 0.42870101195271104 1107 66-67 0.5071514491759075 1107 68-69 0.43117360732384213 1107 70-71 0.44443246894195454 1107 72-73 0.4769137538471462 1107 74-75 0.4954704100741658 1107 76-77 0.4918755587591086 1107 78-79 0.4592615823266186 1107 80-81 0.45548083528408156 1107 82-83 0.4317753587970543 1107 84-85 0.6070860413632104 1107 86-87 0.4899602339920115 1107 88-89 0.5273923306114057 1107 90-91 0.5010373896586522 1107 92-93 0.5389302317248159 1107 94-95 0.34122354374130026 1107 96-97 0.37268126791288125 1107 98-99 0.602930739954882 1107 100 0.5294303700387708 1108 1 0.43332411192042386 1108 2 3.5183446573738983 1108 3 1.936743619907812 1108 4 0.9391100502636931 1108 5 0.3956232912128499 1108 6 0.05650651570806531 1108 7 0.1141639109252921 1108 8 -0.03343644284810665 1108 9 0.011196275235981545 1108 10-11 0.09600662843735108 1108 12-13 -0.006774919807014612 1108 14-15 0.003719611638366871 1108 16-17 -0.018471180667269493 1108 18-19 0.09112664068981502 1108 20-21 0.1648884243342863 1108 22-23 -0.12094195412648645 1108 24-25 -0.10010508687820163 1108 26-27 -0.1411576902156142 1108 28-29 -0.0625467523931107 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2210 78-79 0.2813236467817646 2210 80-81 0.21918710218377058 2210 82-83 0.3442353079056275 2210 84-85 0.2962176171287787 2210 86-87 0.1610503601763611 2210 88-89 0.32551454147557024 2210 90-91 0.07568902247097853 2210 92-93 0.444867656157097 2210 94-95 0.23448723111627245 2210 96-97 0.4152997443577391 2210 98-99 0.3470577541781026 2210 100 0.29620159495028986 2211 1 0.5811364977443212 2211 2 3.9698426478744473 2211 3 2.306674200835836 2211 4 1.123344613654261 2211 5 0.6558991406830756 2211 6 0.5829953721201946 2211 7 0.580375395519134 2211 8 0.6110800594049692 2211 9 0.47092255705305774 2211 10-11 0.49610634223591177 2211 12-13 0.6219738623145261 2211 14-15 0.5240201231473236 2211 16-17 0.5397129625552353 2211 18-19 0.6892419737795734 2211 20-21 0.6941479542332019 2211 22-23 0.6775676671133084 2211 24-25 0.7486724676218586 2211 26-27 0.6613899592789636 2211 28-29 0.5314772702594297 2211 30-31 0.44786005323073397 2211 32-33 0.5052181010158421 2211 34-35 0.7822545688889662 2211 36-37 0.6016102561797254 2211 38-39 0.4205466503042814 2211 40-41 0.4233123399782315 2211 42-43 0.7049666629298628 2211 44-45 0.6158901607871812 2211 46-47 0.6943083809555333 2211 48-49 0.6209860148403763 2211 50-51 0.7380186705299039 2211 52-53 0.4946731669622224 2211 54-55 0.6429019922676389 2211 56-57 0.6429346651833754 2211 58-59 0.6616416746624552 2211 60-61 0.4478602713952853 2211 62-63 0.7696332251361824 2211 64-65 0.5258938085628841 2211 66-67 0.6807228996794663 2211 68-69 0.5595319652211685 2211 70-71 0.5938160663381709 2211 72-73 0.6129176533804319 2211 74-75 0.5756296154291114 2211 76-77 0.804978144118973 2211 78-79 0.6808594698235524 2211 80-81 0.3928234722283186 2211 82-83 0.15653969802574608 2211 84-85 0.5586873064086504 2211 86-87 0.53902680240272 2211 88-89 0.7179167717806507 2211 90-91 0.6537831830755003 2211 92-93 0.6776169794526474 2211 94-95 0.5766194845420891 2211 96-97 0.6869736714992243 2211 98-99 0.7162705213353782 2211 100 0.6408203847771645 2212 1 0.6960700639597768 2212 2 4.224201390310977 2212 3 2.106655487093711 2212 4 1.0686576009913011 2212 5 0.5216865213829678 2212 6 0.5332947919941944 2212 7 0.5486919904497896 2212 8 0.47806693899243413 2212 9 0.33088268638580587 2212 10-11 0.46343591722603605 2212 12-13 0.45976535483943337 2212 14-15 0.41292872211182896 2212 16-17 0.4594251607961439 2212 18-19 0.5379010821737253 2212 20-21 0.5284234724998953 2212 22-23 0.5847098187777959 2212 24-25 0.5738867659604949 2212 26-27 0.38908031962245815 2212 28-29 0.4127171336491102 2212 30-31 0.3303111115968207 2212 32-33 0.4837960645448476 2212 34-35 0.5511775410470747 2212 36-37 0.44299764623649196 2212 38-39 0.1455975932689597 2212 40-41 0.22062236950436187 2212 42-43 0.3497395508761372 2212 44-45 0.5124583983685298 2212 46-47 0.4940667917429664 2212 48-49 0.5007326775563641 2212 50-51 0.4830835708272403 2212 52-53 0.22394509842833799 2212 54-55 0.3005600714059753 2212 56-57 0.2674960618523343 2212 58-59 0.4056890152317649 2212 60-61 0.4043345763876971 2212 62-63 0.5866648097742839 2212 64-65 0.24908247427307373 2212 66-67 0.2798056664022752 2212 68-69 0.4267839561554041 2212 70-71 0.4810235341609257 2212 72-73 0.3950416111289563 2212 74-75 0.8667069291753968 2212 76-77 0.4840153740692763 2212 78-79 0.5161116115073696 2212 80-81 0.3830020065264499 2212 82-83 0.5605882341952508 2212 84-85 0.7371098419127797 2212 86-87 0.6883384225969245 2212 88-89 0.49637790437791196 2212 90-91 0.6008473348231576 2212 92-93 0.6048133749779829 2212 94-95 0.531445940575189 2212 96-97 0.3894180241077123 2212 98-99 0.5349707479795889 2212 100 0.5824413067408614 2213 1 -0.8676212662308345 2213 2 -25.524722569516857 2213 3 -14.518913642997349 2213 4 -6.297684667623464 2213 5 -2.3111594218723823 2213 6 -1.6616807592019 2213 7 -1.6904579378142017 2213 8 -1.236542685345107 2213 9 -1.2791698712226065 2213 10-11 -1.4552763847679344 2213 12-13 -1.4662211983184221 2213 14-15 -1.691208359550103 2213 16-17 -1.95593366566127 2213 18-19 -1.5349612944842548 2213 20-21 -1.6816376861098945 2213 22-23 -1.6838546191925161 2213 24-25 -1.6039691324964451 2213 26-27 -1.6154974261099753 2213 28-29 -1.7859861281434135 2213 30-31 -1.6527019106852663 2213 32-33 -1.6195138569847458 2213 34-35 -1.641593774796334 2213 36-37 -1.7911637012293227 2213 38-39 -1.7326717022541303 2213 40-41 -1.8187665567978613 2213 42-43 -1.8262186372771723 2213 44-45 -1.7180347135103702 2213 46-47 -1.8275786347203038 2213 48-49 -2.0167136302350457 2213 50-51 -2.130927277414493 2213 52-53 -2.1540716570543417 2213 54-55 -2.088704780876398 2213 56-57 -2.014258103228336 2213 58-59 -2.0331139449767086 2213 60-61 -1.8630961764012213 2213 62-63 -1.8805534799806445 2213 64-65 -1.9964276200085465 2213 66-67 -1.7882858994923758 2213 68-69 -2.1252136721642216 2213 70-71 -2.0267658620853766 2213 72-73 -2.101719288352786 2213 74-75 -2.320850715833828 2213 76-77 -1.754508694094575 2213 78-79 -1.871917075577219 2213 80-81 -1.9173843065353573 2213 82-83 -1.7699336086882447 2213 84-85 -1.9287147196191512 2213 86-87 -1.8520926888720517 2213 88-89 -1.51258086205549 2213 90-91 -1.4685179112752813 2213 92-93 -1.69207763752458 2213 94-95 -1.9672931892230707 2213 96-97 -1.93787844325972 2213 98-99 -1.8487620134176517 2213 100 -1.9016596507152919 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 10.0 4 12.0 5 14.0 6 13.0 7 20.0 8 18.0 9 28.0 10 144.0 11 762.0 12 2259.0 13 4344.0 14 5892.0 15 6658.0 16 7060.0 17 7471.0 18 7711.0 19 8109.0 20 9178.0 21 10312.0 22 11392.0 23 11502.0 24 11209.0 25 11314.0 26 12210.0 27 13396.0 28 15713.0 29 18498.0 30 22080.0 31 26737.0 32 33258.0 33 43267.0 34 58179.0 35 79911.0 36 117301.0 37 179133.0 38 140613.0 39 1437.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 39.69500791253057 20.047417637749966 8.838670694864048 31.418903754855414 2 13.44714168506801 23.610160949874498 33.368310087182195 29.574387277875296 3 16.24160507634236 27.467175577740612 28.204549418753782 28.086669927163243 4 11.144630167378661 17.90031615082663 34.53659904710111 36.41845463469359 5 11.891543786010674 37.76099531618599 34.22666828046249 16.120792617340847 6 32.26640657974288 36.5212233211275 16.923673613725292 14.288696485404332 7 25.959004175310586 32.921200564311306 22.90022707029143 18.219568190086687 8 26.055332379798752 34.550230513562774 21.13219684786105 18.262240258777425 9 26.23877110621427 14.688693319464024 21.494577738846385 37.577957835475324 10-11 23.951443565063364 26.82853665741663 27.27324571458552 21.946774062934487 12-13 24.901284724281563 24.924495075071512 28.194588281453164 21.97963191919376 14-15 23.505023176874225 25.172286754507628 25.627326812176072 25.69536325644208 16-17 21.519731533673763 29.02162043491773 27.633670056709803 21.824977974698704 18-19 21.876596569656055 27.616207462822732 29.745917343308093 20.76127862421312 20-21 22.757973455454813 27.077705341636438 28.544109580918214 21.620211621990535 22-23 21.19913882952884 27.237994129627136 28.20589865282469 23.356968388019332 24-25 21.810120996295193 27.024216363406232 27.990613123154866 23.17504951714371 26-27 22.30662227492475 26.496054911976646 28.502348809632398 22.694974003466204 28-29 21.182437613778703 26.35960028575452 29.70187912674969 22.756082973717085 30-31 22.57477249104418 27.137210093922814 28.22188658676414 22.066130828268868 32-33 20.333034447495375 27.362304928538162 29.184121466596512 23.120539157369947 34-35 20.63563273691579 26.76941511253799 29.853350601851957 22.74160154869426 36-37 22.685042673653154 27.136361874402876 28.275656066088867 21.902939385855102 38-39 22.222684603777328 26.688473340884723 28.60153585417423 22.48730620116372 40-41 21.7645180966714 26.72549723804149 28.71285322570531 22.797131439581804 42-43 22.092449209713518 26.813987665989803 28.90904779115413 22.184515333142553 44-45 22.805344327395634 25.799086627837042 28.190546010937435 23.205023033829885 46-47 21.838495917044423 27.097962997698406 29.135307374747647 21.928233710509527 48-49 22.18494467191733 27.280647686666352 28.657591952610627 21.876815688805692 50-51 21.85703802827903 27.107856357251553 27.98186191863863 23.053243695830783 52-53 20.823751654282947 27.455993650290427 27.53781686538969 24.182437830036932 54-55 21.787134225505415 27.226776337958785 27.108350956497347 23.877738480038452 56-57 22.165342838442786 26.50375939849624 27.396288719980117 23.934609043080858 58-59 21.175475243550917 25.855335771883098 28.77452612471266 24.194662859853324 60-61 22.27522222374248 26.721806431604346 27.398340334122345 23.60463101053083 62-63 20.528620647779512 27.291541830177522 27.850562241691808 24.32927528035116 64-65 20.370814863381746 26.390455964309638 28.381989481035934 24.85673969127268 66-67 21.8412548547398 26.5333908034181 27.470301421472378 24.155052920369723 68-69 22.08703694306158 26.255055430842734 27.367891677932633 24.290015948163056 70-71 21.53265089567804 26.273355566819912 27.262971893778747 24.931021643723298 72-73 21.711239398474806 26.567856888318726 27.7131494547787 24.007754258427767 74-75 22.223622302248515 25.41325801029496 27.340541021331138 25.02257866612539 76-77 21.253700845671386 26.50988517044842 28.27808124443056 23.95833273944963 78-79 21.539978905960492 26.443829993443746 28.003135600467488 24.013055500128274 80-81 21.146644242692016 26.287644484984963 27.65788235964825 24.90782891267477 82-83 20.171876647863797 26.620186812529038 27.52888126259502 25.679055277012143 84-85 20.67133568779698 25.972071386425633 27.115953058949955 26.24063986682743 86-87 21.20835525749464 25.968487355435926 27.244772833384545 25.578384553684884 88-89 20.529731104058722 25.36420296059739 28.16626410279944 25.93980183254445 90-91 21.2186890740973 25.505079296309386 27.452826961885325 25.82340466770799 92-93 19.737242638130887 26.137385833881527 27.759624954823792 26.365746573163797 94-95 19.5995207199689 25.591262145884 28.22627626549195 26.582940868655154 96-97 20.625729634088504 25.590718598372913 27.681234267264966 26.102317500273614 98-99 20.40065757822422 25.238497511280748 27.595655514741928 26.765189395753104 100 20.097241957850404 25.27674581842461 27.716468929938998 26.909543293785987 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 30.0 1 28.0 2 22.5 3 19.0 4 21.0 5 44.5 6 104.5 7 183.0 8 268.5 9 286.5 10 202.5 11 134.0 12 123.5 13 135.5 14 149.5 15 176.0 16 212.0 17 257.5 18 307.0 19 376.0 20 477.5 21 592.0 22 801.0 23 1084.5 24 1459.5 25 2051.5 26 2930.0 27 4140.0 28 5626.0 29 7404.0 30 9411.0 31 11602.0 32 13933.5 33 16431.0 34 18981.5 35 21252.0 36 23491.5 37 25661.5 38 27643.5 39 29060.0 40 30049.5 41 30818.5 42 30699.0 43 30707.5 44 30690.0 45 29845.5 46 29520.0 47 30384.0 48 31754.5 49 35386.0 50 47636.5 51 49010.5 52 34240.0 53 25302.5 54 22324.5 55 20579.5 56 19341.0 57 17916.0 58 16380.5 59 14738.0 60 12738.5 61 11010.5 62 9404.5 63 7563.0 64 5766.5 65 4707.0 66 4245.0 67 3915.0 68 3204.5 69 2384.0 70 1747.5 71 1339.5 72 1129.5 73 942.0 74 786.0 75 678.5 76 465.5 77 335.5 78 243.5 79 85.5 80 30.0 81 13.0 82 4.0 83 3.0 84 2.0 85 0.5 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content warn #Base N-Count 1 0.9453160002599277 2 6.346453981966945 3 0.0 4 0.006156182346121092 5 0.157894676840328 6 0.061219813330870856 7 0.039445168365887 8 0.04890744863862868 9 0.04685538785658831 10-11 0.047197397986928374 12-13 0.04594336084234815 14-15 0.049135455392188714 16-17 0.03813412953291676 18-19 0.032718969135865805 20-21 0.02576476315228457 22-23 0.02519474626838447 24-25 0.022572668602444005 26-27 0.01447842885106257 28-29 0.021717643276593854 30-31 0.025479754710334517 32-33 0.0023370692239904145 34-35 0.004674138447980829 36-37 0.00513015195510091 38-39 0.007467221179091325 40-41 0.008208243128161456 42-43 0.009120270142401618 44-45 0.00997529546825177 46-47 0.019095565610653387 48-49 0.029811883027975286 50-51 0.0259357682174546 52-53 0.03152193367967559 54-55 0.02775982224593492 56-57 0.018810557168703338 58-59 0.01630248287954289 60-61 0.014022415343942487 62-63 0.021261629769473774 64-65 0.020463606132013628 66-67 0.021546638211423823 68-69 0.030039889781535326 70-71 0.029811883027975286 72-73 0.02616377497101464 74-75 0.026562786789744712 76-77 0.019551579117773468 78-79 0.01618847950276287 80-81 0.014763437293012618 82-83 0.00963328533791171 84-85 0.012483369757412214 86-87 0.010317305598591831 88-89 0.009177271830791629 90-91 0.008778260012061558 92-93 0.00524415533188093 94-95 0.002052060782040364 96-97 0.0017100506517003032 98-99 0.001026030391020182 100 0.11423138353358027 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 877167.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 71.14430656944029 #Duplication Level Percentage of deduplicated Percentage of total 1 85.84131785312566 61.0712103366754 2 9.671973154803377 13.762116465134557 3 2.451044501993889 5.23133584395583 4 0.8472796772481432 2.411165004327933 5 0.36778238385580747 1.3082811333938567 6 0.18781779932295742 0.801730025653806 7 0.12326389120933175 0.6138666845597184 8 0.07023286030842817 0.39973345160251905 9 0.05667243236792991 0.3628728812177889 >10 0.33006173799095534 4.645922249524666 >50 0.03166294940117293 1.5373472126117738 >100 0.016069814322297947 1.9547729197901473 >500 0.0025711701599724413 1.3922811939135609 >1k 0.0019283776199793311 2.56402449793886 >5k 1.6069813499827758E-4 0.7358122650704285 >10k+ 1.6069813499827758E-4 1.2075278346292522 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 10562 1.2041036655505737 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 6436 0.7337257329562101 No Hit CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC 3683 0.4198744366808145 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 2944 0.33562594124037953 No Hit GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC 2450 0.27930827311104955 No Hit ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC 2367 0.26984599283830785 No Hit ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG 2248 0.25627959100148545 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG 1681 0.191639676367214 No Hit TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG 1501 0.17111906854681036 No Hit GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT 1235 0.14079417032332497 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1148 0.13087587654346322 No Hit GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT 1086 0.12380766718310197 No Hit CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT 1065 0.12141359627072154 No Hit ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA 1019 0.1161694409388406 No Hit CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTC 981 0.11183731262119984 No Hit CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC 959 0.10932923833203939 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 1.7100506517003035E-4 0.0 0.0 0.0 0.0 16-17 3.420101303400607E-4 0.0 0.0 5.7001688390010114E-5 0.0 18-19 3.420101303400607E-4 0.0 0.0 1.1400337678002023E-4 0.0 20-21 3.420101303400607E-4 0.0 0.0 1.7100506517003035E-4 0.0 22-23 3.420101303400607E-4 0.0 0.0 5.13015195510091E-4 0.0 24-25 3.420101303400607E-4 0.0 0.0 7.980236374601416E-4 0.0 26-27 3.420101303400607E-4 0.0 0.0 0.0023940709123804246 0.0 28-29 3.420101303400607E-4 0.0 0.0 0.012426368069022205 0.0 30-31 3.420101303400607E-4 0.0 0.0 0.048394433443118584 0.0 32-33 3.420101303400607E-4 0.0 0.0 0.12278163679208177 0.0 34-35 3.420101303400607E-4 0.0 0.0 0.24892637319917416 0.0 36-37 3.420101303400607E-4 0.0 0.0 0.4250045886359154 0.0 38-39 3.420101303400607E-4 0.0 0.0 0.6980426760240639 0.0 40-41 3.420101303400607E-4 0.0 0.0 1.0807520118745917 0.0 42-43 3.420101303400607E-4 0.0 0.0 1.3784148286472244 0.0 44-45 3.420101303400607E-4 0.0 0.0 1.7119317074171736 0.0 46-47 3.420101303400607E-4 0.0 0.0 2.128101034352637 0.0 48-49 3.420101303400607E-4 0.0 0.0 2.5221537061927775 0.0 50-51 3.420101303400607E-4 0.0 0.0 2.9405460989754517 0.0 52-53 3.420101303400607E-4 0.0 0.0 3.388978381539661 0.0 54-55 3.420101303400607E-4 0.0 0.0 3.8579882736126643 0.0 56-57 3.420101303400607E-4 0.0 0.0 4.3733975400351355 0.0 58-59 3.420101303400607E-4 0.0 0.0 4.926598925860183 0.0 60-61 3.420101303400607E-4 0.0 0.0 5.476038200251492 0.0 62-63 3.420101303400607E-4 0.0 0.0 6.016186199435227 0.0 64-65 3.420101303400607E-4 0.0 0.0 6.6285553378091056 0.0 66-67 3.420101303400607E-4 0.0 0.0 7.32773804760097 0.0 68-69 3.420101303400607E-4 0.0 0.0 8.088767589295994 0.0 70-71 3.420101303400607E-4 0.0 0.0 9.012422947967718 0.0 72-73 3.420101303400607E-4 0.0 0.0 9.841683510665586 0.0 74-75 3.420101303400607E-4 0.0 0.0 10.64392527306659 0.0 76-77 3.420101303400607E-4 0.0 0.0 11.541986873651197 0.0 78-79 3.420101303400607E-4 0.0 0.0 12.471399402850313 0.0 80-81 3.420101303400607E-4 0.0 0.0 13.501362910369405 0.0 82-83 3.420101303400607E-4 0.0 0.0 14.584508993156376 0.0 84-85 3.420101303400607E-4 0.0 0.0 15.656368741642128 0.0 86-87 3.420101303400607E-4 0.0 0.0 16.758040373155854 0.0 88 3.420101303400607E-4 0.0 0.0 17.68534383988454 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGATCCG 65 0.0061531416 28.923306 5 TAGCACG 70 0.008834554 26.857353 4 CTGTCGC 190 1.3505996E-8 24.704588 9 CATGGGT 970 0.0 23.742456 4 ATATACG 110 0.0030791508 21.339432 6 GATCCGT 115 0.0039839684 20.41163 6 ATGGGGG 875 0.0 20.41159 5 TCTGTCG 185 7.254732E-6 20.298983 8 CATGGGG 1730 0.0 19.832527 4 CGACGGT 225 1.8664687E-6 18.776558 7 TATACGC 125 0.0064563225 18.776556 7 CTATACT 230 2.2754866E-6 18.391449 4 TACGACG 205 1.7401422E-5 18.341608 5 GTCTCGC 165 0.0010088331 18.257765 1 GCCTAGA 195 1.4882079E-4 18.023691 1 AGCACCG 135 0.009988531 17.407545 5 CTAGCAC 270 5.440779E-7 17.407545 3 TATTCGG 175 0.0015035587 17.202919 2 CTGGACG 165 0.0015708115 17.091045 4 AGTACTT 2390 0.0 17.08648 12-13 >>END_MODULE