FastQCFastQC Report
Wed 25 May 2016
SRR1295092_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295092_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2906008
Sequences flagged as poor quality0
Sequence length100
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC16050.028.746114
ATCAACG16650.028.3860723
GTACATG41400.027.9333341
CAACGCA17600.027.0505775
TACATGG44050.025.398882
GTATCAA19050.024.8044031
AACGCAG19600.023.5295626
GGTATCA13700.023.145141
ACATGGG48350.022.4595533
TATCAAC22700.020.5363562
CATGGGG37950.020.3118294
ACGCAGA22550.019.8878567
CGCAGAG23400.019.7203188
TATACCG2201.8417957E-518.2235475
CTACACT12250.017.913024
GTATTAA17250.016.2193551
TATATAC14650.015.7465893
TATACTG14800.015.2375955
GTATACG2450.003398972815.2263341
GTACTAC7601.8189894E-1214.7254681
Produced by FastQC (version 0.11.5)