FastQCFastQC Report
Wed 25 May 2016
SRR1295092_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295092_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2906008
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA38450.039.5021361
TCAACGC42450.035.653044
CAACGCA42700.035.3342255
ATCAACG42850.035.3183983
GGTATCA22600.035.1505431
AACGCAG46000.032.867576
TATCAAC49250.030.914572
ACGCAGA54400.027.7886057
CGCAGAG54350.027.727798
GTACATG57000.023.9293651
TACATGG61700.021.9257142
GCAGAGT68800.021.4268559
ACATGGG61300.021.1614073
CATGGGG48850.018.3775334
AGAGTAC60600.018.36054210-11
CCGTGCG2102.1645134E-517.8849589
GACCGCG1600.001288905917.6052027
GAGTACT34850.016.50224712-13
CAGAGTA68950.016.3072610-11
GTCTAGG9200.016.2920911
Produced by FastQC (version 0.11.5)