##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1295092_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2906008 Sequences flagged as poor quality 0 Sequence length 100 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.444753765302778 33.0 33.0 33.0 27.0 33.0 2 29.99516828584092 33.0 33.0 33.0 27.0 33.0 3 30.88441428929308 33.0 33.0 33.0 27.0 33.0 4 35.239802161590745 37.0 37.0 37.0 33.0 37.0 5 35.52879930130956 37.0 37.0 37.0 33.0 37.0 6 35.2852332133979 37.0 37.0 37.0 33.0 37.0 7 35.19965980823177 37.0 37.0 37.0 33.0 37.0 8 35.19603249543704 37.0 37.0 37.0 33.0 37.0 9 35.187743805247614 37.0 37.0 37.0 33.0 37.0 10-11 35.16293210479806 37.0 37.0 37.0 33.0 37.0 12-13 35.06908738723362 37.0 37.0 37.0 33.0 37.0 14-15 36.53863840016958 40.0 37.0 40.0 33.0 40.0 16-17 36.5312875945283 40.0 37.0 40.0 33.0 40.0 18-19 36.551603608799425 40.0 37.0 40.0 33.0 40.0 20-21 36.55457245816254 40.0 37.0 40.0 33.0 40.0 22-23 36.516517504425316 40.0 37.0 40.0 33.0 40.0 24-25 36.47746427401439 40.0 37.0 40.0 33.0 40.0 26-27 36.46106273623472 40.0 37.0 40.0 33.0 40.0 28-29 36.40201369025825 40.0 37.0 40.0 33.0 40.0 30-31 36.32600650101445 37.0 37.0 40.0 33.0 40.0 32-33 36.283287072850456 37.0 37.0 40.0 30.0 40.0 34-35 36.1623564009459 37.0 37.0 40.0 27.0 40.0 36-37 36.17015868504147 37.0 37.0 40.0 27.0 40.0 38-39 36.10779856765707 37.0 37.0 40.0 27.0 40.0 40-41 36.04793018463817 37.0 37.0 40.0 27.0 40.0 42-43 35.943765812069344 37.0 37.0 40.0 27.0 40.0 44-45 35.83476852094007 37.0 37.0 40.0 27.0 40.0 46-47 35.74350535167143 37.0 37.0 40.0 27.0 40.0 48-49 35.65441870772551 37.0 37.0 40.0 27.0 40.0 50-51 35.55591949505989 37.0 37.0 40.0 27.0 40.0 52-53 35.44903093866225 37.0 35.0 40.0 27.0 40.0 54-55 35.32111129769774 37.0 33.0 40.0 27.0 40.0 56-57 35.19465552056292 37.0 33.0 40.0 27.0 40.0 58-59 35.05740589840083 37.0 33.0 40.0 27.0 40.0 60-61 34.899390676144044 37.0 33.0 40.0 27.0 40.0 62-63 34.71115926040121 37.0 33.0 40.0 27.0 40.0 64-65 34.52302953054499 37.0 33.0 40.0 27.0 40.0 66-67 34.295874271509234 37.0 33.0 38.5 27.0 40.0 68-69 34.07655863301133 37.0 33.0 37.0 27.0 40.0 70-71 33.85819206278854 37.0 33.0 37.0 27.0 40.0 72-73 33.64664928658145 37.0 33.0 37.0 27.0 40.0 74-75 33.415218230644925 37.0 33.0 37.0 27.0 40.0 76-77 33.18970405449676 37.0 33.0 37.0 22.0 37.0 78-79 32.96561124401585 37.0 33.0 37.0 22.0 37.0 80-81 32.7603766059832 37.0 33.0 37.0 22.0 37.0 82-83 32.550556639899135 37.0 33.0 37.0 22.0 37.0 84-85 32.38565017715023 33.0 33.0 37.0 22.0 37.0 86-87 32.41613202716579 33.0 33.0 37.0 22.0 37.0 88-89 32.43927477143903 33.0 33.0 37.0 22.0 37.0 90-91 32.46061177395245 33.0 33.0 37.0 22.0 37.0 92-93 32.44443236219584 33.0 33.0 37.0 22.0 37.0 94-95 32.4760988269819 33.0 33.0 37.0 22.0 37.0 96-97 32.445717286394256 33.0 33.0 37.0 22.0 37.0 98-99 32.373336721715845 33.0 33.0 37.0 22.0 37.0 100 31.845645985833485 33.0 33.0 37.0 22.0 37.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.667129157571793 1101 2 4.140539758020054 1101 3 2.333942246108819 1101 4 1.2062641679571868 1101 5 0.5270063608618472 1101 6 0.6062440131886575 1101 7 0.5981799442402931 1101 8 0.5804177084629814 1101 9 0.6708778096895145 1101 10-11 0.6746345494071377 1101 12-13 0.6885520141542045 1101 14-15 1.0348992293420167 1101 16-17 1.0459273690201982 1101 18-19 1.0519016195432869 1101 20-21 0.9887905979489702 1101 22-23 0.9508369636467577 1101 24-25 0.9838301430514278 1101 26-27 0.9361684425987633 1101 28-29 0.8547048857485251 1101 30-31 0.7787607011915441 1101 32-33 0.9859646333338503 1101 34-35 1.1520438245783922 1101 36-37 0.9123293333291826 1101 38-39 1.0157797596571996 1101 40-41 0.9993803761267515 1101 42-43 1.0380218976743762 1101 44-45 1.0413579783002476 1101 46-47 1.0656405572727508 1101 48-49 1.014429969967786 1101 50-51 1.029355991155846 1101 52-53 0.9917672585880482 1101 54-55 0.9572913722624321 1101 56-57 0.9200096345615805 1101 58-59 0.9454972376911641 1101 60-61 0.9087843572229204 1101 62-63 0.9487690195889869 1101 64-65 0.9656327151972945 1101 66-67 0.9511593493692132 1101 68-69 0.9372491289318958 1101 70-71 0.9186735371490542 1101 72-73 0.8876726747888739 1101 74-75 0.8801187821818957 1101 76-77 0.9427475772529661 1101 78-79 0.9555263936607403 1101 80-81 0.8795016827422657 1101 82-83 0.9467184317651487 1101 84-85 0.8612501492354525 1101 86-87 0.9504610760466718 1101 88-89 0.8339869144600485 1101 90-91 0.8363498549670965 1101 92-93 0.9460712834043434 1101 94-95 0.9643283444448585 1101 96-97 0.9279299704398909 1101 98-99 0.8868179350310825 1101 100 1.0539203565479944 1102 1 0.5636073859805251 1102 2 4.062661618190884 1102 3 2.348713976573862 1102 4 1.0917822192067916 1102 5 0.4958440198305283 1102 6 0.5445199511829628 1102 7 0.5111905105167907 1102 8 0.5832437002377375 1102 9 0.6258400621412719 1102 10-11 0.6632983140385846 1102 12-13 0.6793578097884989 1102 14-15 0.9209278025783405 1102 16-17 0.9507719625032749 1102 18-19 0.9593459472876731 1102 20-21 0.8912567289201831 1102 22-23 0.9236816330551747 1102 24-25 0.9324031262845835 1102 26-27 0.9090538079894586 1102 28-29 0.940657128709617 1102 30-31 0.916658556300689 1102 32-33 0.9640264365606797 1102 34-35 1.1039365167644135 1102 36-37 0.9584701495265833 1102 38-39 1.0168829042317 1102 40-41 0.8826381046936618 1102 42-43 1.0178240362381246 1102 44-45 0.9897524610588633 1102 46-47 0.9892447331664513 1102 48-49 0.903355955415428 1102 50-51 0.8669438937183287 1102 52-53 0.9610921304640314 1102 54-55 0.9268439695417712 1102 56-57 0.8772002451278524 1102 58-59 0.8658826600258678 1102 60-61 0.8937725508325371 1102 62-63 0.9557615409429872 1102 64-65 0.8312691593605308 1102 66-67 0.8029771032445723 1102 68-69 0.8159927923295527 1102 70-71 0.8234286652730702 1102 72-73 0.8671713144598598 1102 74-75 0.8015071529506415 1102 76-77 0.7823756949372509 1102 78-79 0.7877409080549214 1102 80-81 0.7203405565163834 1102 82-83 0.7922841641283327 1102 84-85 0.7787825250658855 1102 86-87 0.876337444284566 1102 88-89 0.8437902678292346 1102 90-91 0.856716118807654 1102 92-93 0.9293012739136799 1102 94-95 0.8560340831351496 1102 96-97 0.8533480027081595 1102 98-99 0.8576035345249124 1102 100 0.8401594960608101 1103 1 0.5992943176696386 1103 2 4.058861910256436 1103 3 2.3167640301192094 1103 4 1.071885986852422 1103 5 0.4916371854966002 1103 6 0.5896794644070411 1103 7 0.5389673724346906 1103 8 0.577693857628617 1103 9 0.6051512028195134 1103 10-11 0.6650638048709681 1103 12-13 0.6767572970511893 1103 14-15 0.9535871308275716 1103 16-17 0.908479234935534 1103 18-19 0.9627806122235825 1103 20-21 0.9593366380553476 1103 22-23 0.8876121863571385 1103 24-25 0.9101633729177152 1103 26-27 0.9090967870580968 1103 28-29 0.9710692137331378 1103 30-31 0.9039308327894133 1103 32-33 1.0333811534193487 1103 34-35 1.1223670862922361 1103 36-37 1.0124472775486524 1103 38-39 0.9605943564465278 1103 40-41 0.9316821676500169 1103 42-43 0.8895380586298174 1103 44-45 0.9746640820226773 1103 46-47 1.0211606182873325 1103 48-49 0.9805634322935717 1103 50-51 0.9251115263647662 1103 52-53 0.8967537627859556 1103 54-55 0.9749559150228677 1103 56-57 0.8318382201400283 1103 58-59 0.8153737260651326 1103 60-61 0.8568503635860694 1103 62-63 0.93143623184028 1103 64-65 0.9267316938404946 1103 66-67 0.8514523003888215 1103 68-69 0.8381249098048045 1103 70-71 0.8689117611679293 1103 72-73 0.8951833865026941 1103 74-75 0.7826885386394338 1103 76-77 0.8014596086156516 1103 78-79 0.8170187532601716 1103 80-81 0.7963094937222834 1103 82-83 0.8294841900897154 1103 84-85 0.8316352396529325 1103 86-87 0.8605802481138767 1103 88-89 0.851334126339701 1103 90-91 0.8505564433249333 1103 92-93 0.9621310646000296 1103 94-95 0.8392541512837752 1103 96-97 0.8097700049797822 1103 98-99 0.9230673930353746 1103 100 0.9780151897164977 1104 1 0.5595995516065528 1104 2 3.709755407214871 1104 3 2.2324988204779004 1104 4 1.0876201160191457 1104 5 0.5011171318910002 1104 6 0.5386503751067195 1104 7 0.43510664590949233 1104 8 0.47769638508821544 1104 9 0.4891066280301999 1104 10-11 0.535857604212751 1104 12-13 0.5449586773533994 1104 14-15 0.7149119760913223 1104 16-17 0.6852310883659101 1104 18-19 0.730070968648171 1104 20-21 0.7133669096628665 1104 22-23 0.6944650704524804 1104 24-25 0.7706385518514551 1104 26-27 0.73010332491355 1104 28-29 0.704349695255786 1104 30-31 0.676096426823193 1104 32-33 0.7707876327486431 1104 34-35 0.8580487744048995 1104 36-37 0.7479349671407789 1104 38-39 0.7188942351551333 1104 40-41 0.6743154494192609 1104 42-43 0.7672550976906365 1104 44-45 0.8310845412428947 1104 46-47 0.7844217295515961 1104 48-49 0.6859114861416842 1104 50-51 0.6374231313128078 1104 52-53 0.5820842566552926 1104 54-55 0.6870355962259609 1104 56-57 0.6954691376681126 1104 58-59 0.747617080006151 1104 60-61 0.7073329057454529 1104 62-63 0.680169795954491 1104 64-65 0.6355713611350993 1104 66-67 0.6745344611150941 1104 68-69 0.6439003309427633 1104 70-71 0.5308705055622553 1104 72-73 0.5328704271775706 1104 74-75 0.5913591674100616 1104 76-77 0.5852957606618574 1104 78-79 0.5681263697703329 1104 80-81 0.6051227320010995 1104 82-83 0.5913250316932306 1104 84-85 0.6029803824434694 1104 86-87 0.6992005399138819 1104 88-89 0.5854336460743852 1104 90-91 0.5614572032637852 1104 92-93 0.628736042234415 1104 94-95 0.6495530052514837 1104 96-97 0.566498446349307 1104 98-99 0.5301540964703619 1104 100 0.4943727855777347 1105 1 0.5202827970952484 1105 2 3.7946309389578623 1105 3 2.1482778114779855 1105 4 0.9818797427687045 1105 5 0.2568648583095694 1105 6 0.40656750399413966 1105 7 0.29231992228149295 1105 8 0.2885011848006229 1105 9 0.3661761817382825 1105 10-11 0.3332056523551188 1105 12-13 0.3999187977637959 1105 14-15 0.49891992477528646 1105 16-17 0.498936304394725 1105 18-19 0.5264000129360298 1105 20-21 0.4816349934635724 1105 22-23 0.48452531196319626 1105 24-25 0.5263535975228066 1105 26-27 0.5013149454610257 1105 28-29 0.505387944439228 1105 30-31 0.4766553583806328 1105 32-33 0.4607617241784894 1105 34-35 0.6965300819252818 1105 36-37 0.58861904780413 1105 38-39 0.5435266221387494 1105 40-41 0.4811009711219967 1105 42-43 0.6014139224909698 1105 44-45 0.6382195653812559 1105 46-47 0.576456432873961 1105 48-49 0.5219054256786748 1105 50-51 0.5148381023631146 1105 52-53 0.4614056046271813 1105 54-55 0.5535120488397922 1105 56-57 0.4024649055113514 1105 58-59 0.4167821595383785 1105 60-61 0.44300239808635666 1105 62-63 0.47560375088773554 1105 64-65 0.4611691918597174 1105 66-67 0.4152762777652086 1105 68-69 0.4587275859597071 1105 70-71 0.4723533581557504 1105 72-73 0.5032920814406836 1105 74-75 0.4506009196064724 1105 76-77 0.5067834427435542 1105 78-79 0.5067605505638539 1105 80-81 0.4919597440366985 1105 82-83 0.5048649376089926 1105 84-85 0.5908862364730894 1105 86-87 0.5668943503619204 1105 88-89 0.47562361199563696 1105 90-91 0.451777438274668 1105 92-93 0.4554046213299898 1105 94-95 0.44805849652481555 1105 96-97 0.4764371154803442 1105 98-99 0.4540842105080074 1105 100 0.3264781700939565 1106 1 0.46423298956682046 1106 2 3.8761391718252085 1106 3 2.242115859378856 1106 4 0.9541318573752235 1106 5 0.35466914951352635 1106 6 0.27883685159923033 1106 7 0.2400040872058682 1106 8 0.2021007991810606 1106 9 0.30266675442433666 1106 10-11 0.2484001776312823 1106 12-13 0.25142948446028157 1106 14-15 0.33267107507273863 1106 16-17 0.2887342807869757 1106 18-19 0.31099200556496953 1106 20-21 0.2040515031757053 1106 22-23 0.2552967467279217 1106 24-25 0.18889508914080722 1106 26-27 0.2643930587099206 1106 28-29 0.2052121335126884 1106 30-31 0.24303890852610976 1106 32-33 0.3503964397627257 1106 34-35 0.42905006143300284 1106 36-37 0.3208549846210431 1106 38-39 0.23470207810297694 1106 40-41 0.24466940792773073 1106 42-43 0.28410386973155966 1106 44-45 0.4107530118113445 1106 46-47 0.2813963822218497 1106 48-49 0.2159897306315699 1106 50-51 0.13883551075323197 1106 52-53 0.1431515092664668 1106 54-55 0.24088505823853268 1106 56-57 0.14334710391747763 1106 58-59 0.22325961807901962 1106 60-61 0.11507058477426568 1106 62-63 0.23635184513766916 1106 64-65 0.19902055225198723 1106 66-67 0.13566728576137876 1106 68-69 0.1112476882629494 1106 70-71 0.04482691055005006 1106 72-73 0.06003599585595509 1106 74-75 0.10898409179149837 1106 76-77 0.22122659471310868 1106 78-79 0.14143625227521994 1106 80-81 0.12748809414046747 1106 82-83 0.07981633191570836 1106 84-85 0.0830184776755516 1106 86-87 0.20729050224166912 1106 88-89 0.3508568064139297 1106 90-91 0.21762047999881418 1106 92-93 0.3396214788448333 1106 94-95 0.3885069873938747 1106 96-97 0.2588474583597815 1106 98-99 0.26849144999081176 1106 100 0.19524545187252684 1107 1 0.4598317281710642 1107 2 3.578803160640657 1107 3 2.1576363874617392 1107 4 0.9944229969125615 1107 5 0.2719011034338976 1107 6 0.27387761806565436 1107 7 0.06519362133985851 1107 8 0.13937430295970898 1107 9 0.16034437840828275 1107 10-11 0.1925678668577646 1107 12-13 0.20675796532965052 1107 14-15 0.2236814253910424 1107 16-17 0.1526994544021747 1107 18-19 0.2160188183181333 1107 20-21 0.27726857597138377 1107 22-23 0.1991962150383273 1107 24-25 0.2624746752152376 1107 26-27 0.2413212610030726 1107 28-29 0.17072937757546214 1107 30-31 0.14841451223068702 1107 32-33 0.20356909372205223 1107 34-35 0.39344630289532745 1107 36-37 0.15619953384285168 1107 38-39 0.1358430338493264 1107 40-41 0.20118584286604602 1107 42-43 0.2874019493943152 1107 44-45 0.4122822860750688 1107 46-47 0.23239029376269116 1107 48-49 0.18731673312120023 1107 50-51 0.19250016073063847 1107 52-53 0.1178543080401866 1107 54-55 0.2103845528210968 1107 56-57 0.20843630475196306 1107 58-59 0.25217419599475477 1107 60-61 0.2874758301585203 1107 62-63 0.3468110030298348 1107 64-65 0.17820149895872106 1107 66-67 0.16168030479998663 1107 68-69 0.17347992129909073 1107 70-71 0.17439141316678075 1107 72-73 0.22622666118701318 1107 74-75 0.18096579196554785 1107 76-77 0.20958292760088426 1107 78-79 0.1329340123971079 1107 80-81 0.2599304204178736 1107 82-83 0.2870679360013071 1107 84-85 0.2758298873471503 1107 86-87 0.21441093747964857 1107 88-89 0.14599104553500553 1107 90-91 0.18717115445249988 1107 92-93 0.30649181182683094 1107 94-95 0.21966861327124576 1107 96-97 0.12803484651892205 1107 98-99 0.04031018390922725 1107 100 0.10270067558122165 1108 1 0.43319275291664994 1108 2 3.6304668488138034 1108 3 2.0322176443500517 1108 4 0.9433072406047955 1108 5 0.26799159313850396 1108 6 0.044676147093568375 1108 7 -0.09017287512277505 1108 8 0.036385840038022366 1108 9 -0.020899186551069704 1108 10-11 0.04813631689165021 1108 12-13 0.034191336496370184 1108 14-15 -0.06940982303586196 1108 16-17 -0.02362357403183779 1108 18-19 0.01466392478013745 1108 20-21 -0.009292226540267734 1108 22-23 -0.0434015814003601 1108 24-25 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0.19193134059398886 2211 98-99 0.24509512698598712 2211 100 0.0945539456647424 2212 1 0.44058557159053535 2212 2 3.986648270954177 2212 3 2.205219336323598 2212 4 1.0711433870900038 2212 5 0.5428544890693203 2212 6 0.3914745288680521 2212 7 0.48756006213305625 2212 8 0.4393956918957116 2212 9 0.18979217892959355 2212 10-11 0.3578123494921783 2212 12-13 0.370928946389121 2212 14-15 0.4981456545074323 2212 16-17 0.3310582607305932 2212 18-19 0.2869925791959105 2212 20-21 0.38348888741862197 2212 22-23 0.26765660698634974 2212 24-25 0.18806026914172236 2212 26-27 0.15082840652960527 2212 28-29 0.23947698272422002 2212 30-31 0.1289262518168499 2212 32-33 0.33926330830207263 2212 34-35 0.2702105522180105 2212 36-37 0.27833534243586655 2212 38-39 0.15240974469050883 2212 40-41 0.3920563320137873 2212 42-43 0.3080214919211315 2212 44-45 0.3509117519869207 2212 46-47 0.4313060470350578 2212 48-49 0.4041570398784131 2212 50-51 0.3956447904857967 2212 52-53 0.20887056633473122 2212 54-55 0.22412922501622745 2212 56-57 0.39263415545110547 2212 58-59 0.40557392175684015 2212 60-61 0.3866840002590237 2212 62-63 0.3343352218606057 2212 64-65 0.3717496598819068 2212 66-67 0.4924760813467586 2212 68-69 0.4619952115162036 2212 70-71 0.3457308133439767 2212 72-73 0.48152084522106975 2212 74-75 0.5241555783938168 2212 76-77 0.4835882913918894 2212 78-79 0.32874870784636556 2212 80-81 0.3416294824809327 2212 82-83 0.46552262085408813 2212 84-85 0.5405938285069638 2212 86-87 0.3032521471000038 2212 88-89 0.38420778607741113 2212 90-91 0.3447021042091123 2212 92-93 0.4268925147895857 2212 94-95 0.6017577953353523 2212 96-97 0.34360702252046593 2212 98-99 0.6188711883309566 2212 100 0.7029494022130471 2213 1 -0.7167512393338065 2213 2 -25.932538381809117 2213 3 -14.871838177659166 2213 4 -6.363447688588682 2213 5 -2.0979446809238667 2213 6 -1.4706159415244997 2213 7 -1.3363226495837992 2213 8 -1.1843711195443234 2213 9 -1.243021496953446 2213 10-11 -1.1197411494987222 2213 12-13 -1.1782486948217255 2213 14-15 -1.4153958856027202 2213 16-17 -1.3869856904724216 2213 18-19 -1.425475310046778 2213 20-21 -1.4223180573329586 2213 22-23 -1.4238087273807025 2213 24-25 -1.4191723976047754 2213 26-27 -1.3974443646844747 2213 28-29 -1.5090071797564377 2213 30-31 -1.4577157605476003 2213 32-33 -1.2147411074460237 2213 34-35 -1.2259934077955208 2213 36-37 -1.4058689619570686 2213 38-39 -1.5484528208311659 2213 40-41 -1.4632947033532773 2213 42-43 -1.4037646309873608 2213 44-45 -1.4012454266192549 2213 46-47 -1.316619810002031 2213 48-49 -1.3760233572417349 2213 50-51 -1.2055420073713634 2213 52-53 -1.49719702293077 2213 54-55 -1.4941039600474966 2213 56-57 -1.4093594866407955 2213 58-59 -1.4369339032599626 2213 60-61 -1.5273153284633523 2213 62-63 -1.3140370173135096 2213 64-65 -1.4604143276470083 2213 66-67 -1.5203832991024555 2213 68-69 -1.5636931758179742 2213 70-71 -1.4307947043832598 2213 72-73 -1.490840085372497 2213 74-75 -1.6012894270996512 2213 76-77 -1.5790712790778372 2213 78-79 -1.4788299553147404 2213 80-81 -1.5262896530393562 2213 82-83 -1.4068194737593487 2213 84-85 -1.4125434766105656 2213 86-87 -1.3649514073499631 2213 88-89 -1.3919928000668236 2213 90-91 -1.31593843274306 2213 92-93 -1.3272955408390494 2213 94-95 -1.4896557073091117 2213 96-97 -1.470787118834167 2213 98-99 -1.3701337999260517 2213 100 -1.3390500261521012 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 8.0 4 25.0 5 43.0 6 45.0 7 55.0 8 48.0 9 92.0 10 375.0 11 1629.0 12 4806.0 13 8800.0 14 11742.0 15 13273.0 16 14540.0 17 15190.0 18 16118.0 19 17296.0 20 18663.0 21 20965.0 22 23165.0 23 25002.0 24 26093.0 25 28286.0 26 30990.0 27 35059.0 28 42101.0 29 49691.0 30 60601.0 31 74597.0 32 95529.0 33 128828.0 34 176773.0 35 253950.0 36 392428.0 37 674714.0 38 641322.0 39 3165.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 38.61816152358239 20.222269982459668 10.21973946020147 30.93982903375647 2 13.2837899924742 22.396516566085058 36.238327056958155 28.081366384482592 3 15.425456502528553 26.301304056974377 29.16340904773834 29.109830392758727 4 10.738039545784757 18.019393312861965 37.23816154119608 34.0044056001572 5 11.706646702500139 38.57275511702407 35.32320096732453 14.397397213151256 6 30.286143587910814 39.46748033401954 17.423161768376808 12.82321430969284 7 26.573260712562057 33.02888789288918 22.87333981880608 17.52451157574268 8 24.74999793443625 36.88033087577149 20.480030129689865 17.889641060102395 9 25.06193154825428 16.645472008592616 21.340737119683837 36.951859323469264 10-11 23.462970453821725 27.263626270537305 29.182105326574348 20.09129794906662 12-13 24.076531990142605 24.656687521053335 29.299928446623884 21.966852042180175 14-15 21.435670628218432 26.41982763428504 26.261156710688756 25.88334502680778 16-17 21.155890044043037 29.680538671349172 27.006407402135522 22.15716388247227 18-19 21.112786867075837 28.81607238715023 28.928389916343576 21.14275082943036 20-21 22.179779105721853 28.193061950180127 28.312618220361806 21.314540723736215 22-23 21.755729000662924 27.94289138562341 28.520162764266484 21.781216849447183 24-25 21.677819362806005 27.93259921264558 28.429755223502507 21.959826201045903 26-27 21.469663966971787 28.05051009468836 28.458716625648695 22.02110931269116 28-29 21.44379654193989 27.94870594649217 28.69342861194365 21.914068899624294 30-31 21.75339292536769 28.281474266085205 28.230085601880695 21.735047206666415 32-33 21.330368402008347 28.1768098391948 28.710022593148345 21.782799165648502 34-35 21.50240002229938 27.86412382624998 28.615007241276015 22.018468910174622 36-37 21.760907812157214 28.160398501318447 28.281893225391553 21.796800461132786 38-39 21.588436198788884 28.258018845929033 28.45491635512306 21.698628600159026 40-41 21.662282922048266 28.041861635185494 28.391798365413308 21.90405707735293 42-43 21.408973988143405 28.045799285878857 28.491630301298144 22.053596424679593 44-45 21.55357256854328 28.00653595333566 28.46846115156601 21.971430326555048 46-47 21.670349571671924 28.211952918242606 28.176675500935023 21.941022009150444 48-49 21.591103680990358 28.12879533774838 28.23277801900429 22.04732296225697 50-51 21.80556791290088 28.015927007125917 28.064388834573933 22.114116245399266 52-53 21.663468672536705 28.148876852643696 28.067230332852418 22.120424141967188 54-55 21.76818451231576 28.234431394739683 28.02748216880724 21.969901924137318 56-57 21.76570255642141 28.051405828715154 28.026195420757404 22.15669619410603 58-59 21.487865128128796 27.825549009704627 28.360400575101806 22.32618528706477 60-61 21.746721344057498 28.115693238293193 27.981781698033437 22.15580371961587 62-63 21.61202929160108 28.187908721966064 28.112998410414548 22.087063576018306 64-65 21.590180438317972 27.8006659955084 28.139686964901866 22.46946660127176 66-67 21.51572583885797 27.964026215381843 28.13286737235421 22.387380573405977 68-69 21.693492036720237 28.02170927402838 28.07356438949336 22.21123429975802 70-71 21.7369678874593 27.82421384632807 28.02738479320967 22.411433473002962 72-73 21.548488358284988 28.00718346048666 28.148047437686834 22.296280743541512 74-75 21.649440002918823 27.91442348301353 28.006669229813415 22.429467284254237 76-77 21.645733323811005 28.042275686833634 27.88169828968121 22.430292699674148 78-79 21.61344584089421 28.018684721566157 28.038439796495197 22.329429641044438 80-81 21.73052715988942 27.85920720810637 27.922550025941128 22.487715606063084 82-83 21.54329067608337 27.93043537513527 28.156933895229074 22.369340053552282 84-85 21.542623669591276 27.776435581821296 28.024862287253338 22.656078461334086 86-87 21.51605797189231 28.01156877408369 27.979649337022106 22.492723917001893 88-89 21.47600009291785 27.716277056947458 28.09612179809811 22.711601052036578 90-91 21.41382987912716 27.753416379279788 28.129511480734436 22.703242260858612 92-93 21.3313194935186 27.96351825248809 28.0606313071221 22.64453094687121 94-95 21.344660367904947 27.595589490317856 28.155833099332135 22.903917042445066 96-97 21.305173259848136 27.762611743637777 28.1083640573135 22.82385093920059 98-99 21.231193605765743 27.73084794078995 28.073932043194873 22.96402641024944 100 21.366224091947878 27.497173479138866 28.022241498217966 23.114360930695295 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 14.0 1 10.5 2 7.0 3 9.0 4 16.0 5 30.5 6 73.5 7 162.0 8 237.0 9 242.5 10 215.0 11 205.0 12 253.0 13 311.0 14 334.0 15 395.0 16 496.0 17 616.5 18 754.0 19 937.0 20 1248.5 21 1667.0 22 2412.5 23 3810.5 24 5754.5 25 8582.0 26 12700.5 27 18398.5 28 26075.5 29 34702.5 30 43508.5 31 53060.0 32 62222.5 33 70588.0 34 79274.5 35 87510.0 36 94955.5 37 102567.5 38 108866.5 39 113092.5 40 115880.0 41 116479.0 42 115555.5 43 114655.0 44 112339.5 45 108570.0 46 104729.5 47 100251.0 48 96786.5 49 93944.5 50 90943.5 51 87909.0 52 82653.0 53 77194.0 54 72768.0 55 68175.0 56 63406.5 57 57569.5 58 51577.5 59 46311.5 60 40601.0 61 34105.5 62 27878.5 63 22231.5 64 17362.5 65 13496.5 66 10256.0 67 7477.0 68 5387.5 69 3932.0 70 2820.5 71 2018.5 72 1502.0 73 1079.5 74 732.0 75 474.0 76 270.5 77 157.5 78 95.0 79 44.0 80 31.5 81 22.0 82 11.0 83 7.0 84 4.0 85 2.0 86 0.0 87 0.5 88 1.0 89 0.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content warn #Base N-Count 1 0.7304866332095438 2 5.852943281642721 3 0.0 4 0.005540246275990981 5 0.16551915892867466 6 0.055987457708306376 7 0.036269686800586926 8 0.042394928024974465 9 0.04088082345265395 10-11 0.04242933949252721 12-13 0.04072597184866662 14-15 0.04315198031113472 16-17 0.034703965026937296 18-19 0.02930136462115727 20-21 0.02345141513719164 22-23 0.02415685022202279 24-25 0.020027474115694107 26-27 0.012766654462066174 28-29 0.019408067699744805 30-31 0.024019204351811832 32-33 0.001324841500780452 34-35 0.003355118086392054 36-37 0.004146581840105051 38-39 0.006245681360822131 40-41 0.006779059107889586 42-43 0.006830676309218694 44-45 0.0080694891411173 46-47 0.01605294961335275 48-49 0.024655816501537503 50-51 0.02162760735689647 52-53 0.027580791243520322 54-55 0.024294496092233744 56-57 0.015898098009365424 58-59 0.014986194119217842 60-61 0.012336511117656938 62-63 0.018358517939386265 64-65 0.020061885583246845 66-67 0.021145846811158123 68-69 0.027460351107085734 70-71 0.028182991925693256 72-73 0.024879491040630308 74-75 0.024948313975735787 76-77 0.019115570225546524 78-79 0.01464207944369045 80-81 0.013437678079344586 82-83 0.008585661154408385 84-85 0.011080492551981963 86-87 0.008774924225948449 88-89 0.0074156712576152585 90-91 0.007811403134471757 92-93 0.004008935969894095 94-95 0.0013764587021095607 96-97 8.946981563712143E-4 98-99 5.161720132910852E-4 100 0.10825847692091693 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 2906008.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 74.71040139824566 #Duplication Level Percentage of deduplicated Percentage of total 1 83.44715901784323 62.34370745766304 2 10.915583523639267 16.310152530943327 3 2.9180830948744627 6.540334779945183 4 1.1262386097609303 3.3656695442176496 5 0.5175817397197635 1.933436976543292 6 0.299012799054014 1.3403619744323014 7 0.18210024462606045 0.9523347659512192 8 0.11693198700229392 0.6988828548188658 9 0.0877113201441211 0.5897653141623587 >10 0.37031760282158516 4.631499615778678 >50 0.015170279944162394 0.7648433867555451 >100 0.0039710455077707025 0.421318945513825 >500 4.624502076728346E-5 0.02214644785148324 >1k 9.249004152783689E-5 0.08554540542321759 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 3.441146755273902E-5 0.0 0.0 0.0 0.0 8 3.441146755273902E-5 0.0 0.0 0.0 0.0 9 3.441146755273902E-5 0.0 0.0 0.0 0.0 10-11 6.882293510547804E-5 0.0 0.0 0.0 0.0 12-13 6.882293510547804E-5 0.0 0.0 0.0 0.0 14-15 6.882293510547804E-5 0.0 0.0 1.720573377636951E-5 0.0 16-17 6.882293510547804E-5 0.0 0.0 6.882293510547804E-5 0.0 18-19 6.882293510547804E-5 0.0 0.0 6.882293510547804E-5 0.0 20-21 6.882293510547804E-5 0.0 0.0 1.2044013643458656E-4 0.0 22-23 6.882293510547804E-5 1.720573377636951E-5 0.0 1.3764587021095607E-4 0.0 24-25 6.882293510547804E-5 6.882293510547804E-5 0.0 2.7529174042191214E-4 0.0 26-27 6.882293510547804E-5 6.882293510547804E-5 0.0 3.957318768564987E-4 0.0 28-29 6.882293510547804E-5 6.882293510547804E-5 0.0 9.635210914766924E-4 0.0 30-31 6.882293510547804E-5 8.602866888184754E-5 0.0 0.0038196728983540306 0.0 32-33 6.882293510547804E-5 1.0323440265821704E-4 0.0 0.011407401493732983 0.0 34-35 6.882293510547804E-5 1.720573377636951E-4 0.0 0.021851281895989276 0.0 36-37 6.882293510547804E-5 2.0646880531643408E-4 0.0 0.03802467164577661 0.0 38-39 6.882293510547804E-5 2.0646880531643408E-4 0.0 0.062267550536681246 0.0 40-41 6.882293510547804E-5 2.0646880531643408E-4 0.0 0.09213670437245872 0.0 42-43 6.882293510547804E-5 2.0646880531643408E-4 0.0 0.12212629834467076 0.0 44-45 6.882293510547804E-5 2.0646880531643408E-4 0.0 0.16192316056941342 0.0 46-47 6.882293510547804E-5 2.236745390928036E-4 0.0 0.21061538715653916 0.0 48-49 6.882293510547804E-5 2.408802728691731E-4 0.0 0.26292081783670246 0.0 50-51 6.882293510547804E-5 2.408802728691731E-4 0.0 0.31636182694610615 0.0 52-53 6.882293510547804E-5 2.408802728691731E-4 0.0 0.37480970458443336 0.0 54-55 1.2044013643458656E-4 2.408802728691731E-4 0.0 0.4449746869244682 0.0 56-57 1.3764587021095607E-4 2.408802728691731E-4 0.0 0.5245856171077299 0.0 58-59 1.3764587021095607E-4 2.408802728691731E-4 0.0 0.6120939790943453 0.0 60-61 1.3764587021095607E-4 3.441146755273902E-4 0.0 0.7080159448976052 0.0 62-63 1.3764587021095607E-4 3.441146755273902E-4 0.0 0.8058477471500423 0.0 64-65 1.3764587021095607E-4 3.441146755273902E-4 0.0 0.9170656102804948 0.0 66-67 1.3764587021095607E-4 3.441146755273902E-4 0.0 1.0458161161290678 0.0 68-69 1.3764587021095607E-4 3.441146755273902E-4 0.0 1.1870751904330614 0.0 70-71 1.3764587021095607E-4 3.441146755273902E-4 0.0 1.3437678079344586 0.0 72-73 1.3764587021095607E-4 3.441146755273902E-4 0.0 1.5107322485003483 0.0 74-75 1.3764587021095607E-4 3.785261430801292E-4 0.0 1.6893105593652873 0.0 76-77 1.3764587021095607E-4 4.129376106328682E-4 0.0 1.8841827001164484 0.0 78-79 1.3764587021095607E-4 4.473490781856072E-4 0.0 2.1142061549727322 0.0 80-81 1.3764587021095607E-4 4.6455481196197666E-4 0.0 2.3617450468133603 0.0 82-83 1.3764587021095607E-4 4.817605457383462E-4 0.0 2.625147625195801 0.0 84-85 1.3764587021095607E-4 4.817605457383462E-4 0.0 2.8971874819339796 0.0 86-87 1.3764587021095607E-4 4.817605457383462E-4 0.0 3.1993545785145807 0.0 88 1.3764587021095607E-4 4.817605457383462E-4 0.0 3.442144687832931 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 3845 0.0 39.502136 1 TCAACGC 4245 0.0 35.65304 4 CAACGCA 4270 0.0 35.334225 5 ATCAACG 4285 0.0 35.318398 3 GGTATCA 2260 0.0 35.150543 1 AACGCAG 4600 0.0 32.86757 6 TATCAAC 4925 0.0 30.91457 2 ACGCAGA 5440 0.0 27.788605 7 CGCAGAG 5435 0.0 27.72779 8 GTACATG 5700 0.0 23.929365 1 TACATGG 6170 0.0 21.925714 2 GCAGAGT 6880 0.0 21.426855 9 ACATGGG 6130 0.0 21.161407 3 CATGGGG 4885 0.0 18.377533 4 AGAGTAC 6060 0.0 18.360542 10-11 CCGTGCG 210 2.1645134E-5 17.884958 9 GACCGCG 160 0.0012889059 17.605202 7 GAGTACT 3485 0.0 16.502247 12-13 CAGAGTA 6895 0.0 16.30726 10-11 GTCTAGG 920 0.0 16.292091 1 >>END_MODULE