FastQCFastQC Report
Wed 25 May 2016
SRR1295091_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295091_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2211221
Sequences flagged as poor quality0
Sequence length100
%GC41

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACCG1150.002752035221.7581045
AACGCCG1403.3556772E-421.500976
GTATACG1500.003746855820.6301881
CCGACGT550.004264890720.17498672-73
GTATAAG8300.020.1330761
GTACATG54450.019.0957131
TAGCACG1350.00696085418.534685
GTCTCGC3052.4357305E-618.262791
GGACCGT2001.8189335E-417.5591266
TACATGG56200.017.3957962
CTGTCGC4052.3283064E-1017.3587049
TCTGTCG3902.6575435E-916.7310858
TCTAGAC6050.016.7119963
GTATTAG9800.016.4199471
ACATGGG58400.016.3511123
TGGACCG1900.002652861615.8032535
ATACCGT2254.4657796E-415.6081126
TAGACAG13650.015.3980435
GTATCAA37450.014.8735681
CCTATAC6501.0913936E-1114.6908453
Produced by FastQC (version 0.11.5)