##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1295091_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2211221
Sequences flagged as poor quality	0
Sequence length	100
%GC	41
>>END_MODULE
>>Per base sequence quality	warn
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	26.529507905360884	33.0	27.0	33.0	15.0	33.0
2	22.77455849053532	33.0	6.0	33.0	2.0	33.0
3	25.090558564702487	33.0	15.0	33.0	15.0	33.0
4	25.312663003833627	33.0	6.0	37.0	2.0	37.0
5	27.051936916300996	33.0	15.0	37.0	2.0	37.0
6	27.9292146737029	33.0	15.0	37.0	6.0	37.0
7	27.879350820202955	33.0	15.0	37.0	6.0	37.0
8	28.51014303861984	33.0	22.0	37.0	6.0	37.0
9	29.568674049314836	37.0	27.0	37.0	15.0	37.0
10-11	29.092270740916444	35.0	24.5	37.0	10.5	37.0
12-13	27.912377822026833	33.0	15.0	37.0	4.0	37.0
14-15	29.722431407805914	33.0	27.0	37.0	10.5	40.0
16-17	30.156729472088045	37.0	27.0	40.0	6.0	40.0
18-19	30.31350914268633	37.0	27.0	40.0	10.5	40.0
20-21	29.860894727392694	35.0	27.0	40.0	6.0	40.0
22-23	30.57684464827351	37.0	27.0	40.0	15.0	40.0
24-25	30.7292970716179	37.0	27.0	40.0	15.0	40.0
26-27	29.323950432815174	33.0	24.5	40.0	4.0	40.0
28-29	30.02128642953373	35.0	27.0	40.0	10.5	40.0
30-31	30.70119608126008	37.0	27.0	40.0	15.0	40.0
32-33	29.512057139471814	33.0	27.0	40.0	6.0	40.0
34-35	29.129677675818023	33.0	22.0	37.0	6.0	40.0
36-37	29.446386634352695	33.0	27.0	37.0	6.0	40.0
38-39	29.575648928804494	33.0	24.5	37.0	6.0	40.0
40-41	28.651727484498384	33.0	22.0	37.0	6.0	40.0
42-43	29.30178281591935	33.0	22.0	37.0	6.0	40.0
44-45	29.216238223135544	33.0	22.0	37.0	6.0	40.0
46-47	28.93639758305479	33.0	22.0	37.0	6.0	40.0
48-49	28.88947622150839	33.0	22.0	37.0	6.0	40.0
50-51	28.85025196486466	33.0	22.0	37.0	6.0	40.0
52-53	29.03205717565092	33.0	22.0	37.0	6.0	40.0
54-55	29.04409984348014	33.0	22.0	37.0	6.0	40.0
56-57	29.04331860089968	33.0	22.0	37.0	6.0	40.0
58-59	28.91870961789889	33.0	22.0	37.0	10.5	40.0
60-61	28.87662698572418	33.0	22.0	37.0	10.5	40.0
62-63	28.829631457009498	33.0	22.0	37.0	10.5	40.0
64-65	28.428520260977983	33.0	22.0	37.0	6.0	40.0
66-67	28.64423999229385	33.0	22.0	37.0	10.5	40.0
68-69	28.215025544710365	33.0	22.0	37.0	6.0	40.0
70-71	28.366670269502688	33.0	22.0	37.0	10.5	38.5
72-73	28.414544272146472	33.0	22.0	37.0	10.5	37.0
74-75	28.50109961871744	33.0	22.0	37.0	15.0	37.0
76-77	28.284292931371404	33.0	22.0	37.0	15.0	37.0
78-79	27.824778934353464	33.0	22.0	37.0	10.5	37.0
80-81	27.625101018848863	33.0	22.0	37.0	10.5	37.0
82-83	27.733138614367356	33.0	22.0	35.0	15.0	37.0
84-85	27.683790539254105	33.0	22.0	33.0	15.0	37.0
86-87	27.446644636605747	27.0	22.0	33.0	15.0	37.0
88-89	27.396014916645598	27.0	22.0	33.0	15.0	37.0
90-91	27.534862186999852	27.0	22.0	33.0	15.0	37.0
92-93	27.460943297843137	27.0	22.0	33.0	15.0	37.0
94-95	27.334776804308568	27.0	22.0	33.0	15.0	37.0
96-97	27.412706825776347	27.0	22.0	33.0	15.0	37.0
98-99	27.473349113453608	27.0	22.0	33.0	15.0	37.0
100	27.14114373913779	27.0	22.0	33.0	15.0	37.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	7.299096212284319
1101	2	11.234092832797689
1101	3	8.442531972968759
1101	4	12.908574489125133
1101	5	10.721819388431374
1101	6	9.894294623021377
1101	7	9.432505138384325
1101	8	9.108189450542852
1101	9	7.398661602672899
1101	10-11	7.762760422468322
1101	12-13	9.158636273610885
1101	14-15	8.131769738127044
1101	16-17	8.269694546976623
1101	18-19	8.170695910092054
1101	20-21	7.2742473175768865
1101	22-23	7.43230055478848
1101	24-25	7.406026704207019
1101	26-27	8.629222323649142
1101	28-29	8.330663729985165
1101	30-31	5.548555627456757
1101	32-33	7.073865959738537
1101	34-35	8.786264267637794
1101	36-37	8.160008407704463
1101	38-39	8.30648333668552
1101	40-41	9.064477930032268
1101	42-43	8.71556872074311
1101	44-45	8.558455139953221
1101	46-47	7.553438079052778
1101	48-49	5.574046389270578
1101	50-51	6.91629764207763
1101	52-53	6.012792554409067
1101	54-55	4.188312089704326
1101	56-57	6.3767100585907315
1101	58-59	6.425787047434685
1101	60-61	6.862512580211753
1101	62-63	6.468152974795295
1101	64-65	6.914661348771105
1101	66-67	5.868757392643847
1101	68-69	6.874336213373187
1101	70-71	6.40375945228897
1101	72-73	5.614105881439315
1101	74-75	5.064221248811151
1101	76-77	5.109796707913933
1101	78-79	5.212520064035825
1101	80-81	5.042084847861915
1101	82-83	4.518469716200361
1101	84-85	4.355848079321582
1101	86-87	4.1962067368577785
1101	88-89	4.094787717018168
1101	90-91	3.851550599131773
1101	92-93	3.8684997558059884
1101	94-95	3.6960105671327597
1101	96-97	3.8135239514627592
1101	98-99	3.5453664631676247
1101	100	3.726287486319851
1102	1	6.744706409281093
1102	2	10.595888331383723
1102	3	8.059266427889568
1102	4	12.615585609137735
1102	5	10.52425000818035
1102	6	9.65240832925858
1102	7	6.6371155955784396
1102	8	7.800624384089815
1102	9	6.56159477988783
1102	10-11	7.402157380898377
1102	12-13	9.039068492204034
1102	14-15	6.279358718769732
1102	16-17	7.444110215649545
1102	18-19	7.500536607694816
1102	20-21	7.008448545982212
1102	22-23	6.612417540881612
1102	24-25	6.9687732925406
1102	26-27	8.576864969993036
1102	28-29	7.703560154325945
1102	30-31	4.260099830191571
1102	32-33	4.441627595010342
1102	34-35	5.192405267206414
1102	36-37	5.394331004473909
1102	38-39	4.582990427063013
1102	40-41	6.373840583497344
1102	42-43	7.174891745468717
1102	44-45	6.51895958042623
1102	46-47	5.67522429954948
1102	48-49	3.893915280624494
1102	50-51	5.108558311880358
1102	52-53	5.983837897219061
1102	54-55	5.36082925930506
1102	56-57	6.420020676011632
1102	58-59	6.05550954354884
1102	60-61	6.080501802564076
1102	62-63	4.385677607951287
1102	64-65	6.222672944670947
1102	66-67	3.876750672020467
1102	68-69	6.228136036028165
1102	70-71	5.388675709829556
1102	72-73	5.204837121553531
1102	74-75	4.348989262341412
1102	76-77	3.3979466877143985
1102	78-79	4.513680937888047
1102	80-81	4.6507548115027575
1102	82-83	4.008153918798229
1102	84-85	3.118219178929234
1102	86-87	3.320757040535465
1102	88-89	3.5200577573487557
1102	90-91	3.382252344508135
1102	92-93	3.2455767981839223
1102	94-95	3.1587510784890043
1102	96-97	3.3570697735836426
1102	98-99	3.2028664802858735
1102	100	3.236602085444769
1103	1	6.7607169143179995
1103	2	11.015327452724542
1103	3	7.893168766525907
1103	4	12.191295480941502
1103	5	10.471202884865011
1103	6	9.186545667435649
1103	7	8.243428697144662
1103	8	8.143935732011848
1103	9	7.143222324372264
1103	10-11	7.417324369755963
1103	12-13	8.756392538795861
1103	14-15	8.595712213401871
1103	16-17	8.143062893550471
1103	18-19	7.375368851048176
1103	20-21	8.459518735222929
1103	22-23	7.06428733507909
1103	24-25	5.317665485142758
1103	26-27	6.161210014388065
1103	28-29	7.083346170819031
1103	30-31	6.113415574494674
1103	32-33	7.939001642548757
1103	34-35	7.2508812550423904
1103	36-37	4.215269628329498
1103	38-39	6.5447765322550175
1103	40-41	6.999927145225833
1103	42-43	7.660698472632948
1103	44-45	5.820773245388338
1103	46-47	6.615196518908565
1103	48-49	5.708045780570853
1103	50-51	6.553471376424419
1103	52-53	6.056309382095016
1103	54-55	5.026368958906719
1103	56-57	6.095732835406032
1103	58-59	5.492391543488541
1103	60-61	5.14217524610844
1103	62-63	2.997543156520777
1103	64-65	5.207818549376682
1103	66-67	4.294943056464714
1103	68-69	5.2467872182479915
1103	70-71	3.832356893669104
1103	72-73	4.435209813015227
1103	74-75	3.24364264590708
1103	76-77	3.590640745518048
1103	78-79	3.1136063589274308
1103	80-81	4.140650891781938
1103	82-83	3.5323915112922677
1103	84-85	3.381601540325782
1103	86-87	2.88670933033481
1103	88-89	3.077747506418465
1103	90-91	2.8768917571179635
1103	92-93	2.2912502981730505
1103	94-95	2.796952360871998
1103	96-97	3.0476522362301814
1103	98-99	2.763836467797905
1103	100	3.037333045153023
1104	1	6.497935592587872
1104	2	9.882064938765641
1104	3	7.427939002768312
1104	4	12.394355805228361
1104	5	9.674898990264868
1104	6	8.415966516759735
1104	7	4.435448556644463
1104	8	6.875012404904744
1104	9	6.812761060386062
1104	10-11	7.226952009505187
1104	12-13	7.583926909285733
1104	14-15	7.546417426502668
1104	16-17	7.384146875733201
1104	18-19	7.077167951377174
1104	20-21	7.664164663413224
1104	22-23	6.729039229175132
1104	24-25	6.185094292516563
1104	26-27	8.382532598428792
1104	28-29	7.335862218854476
1104	30-31	6.465031217556437
1104	32-33	7.940492680389145
1104	34-35	8.229006330006026
1104	36-37	6.882578431235029
1104	38-39	7.694635278394056
1104	40-41	7.412613578661173
1104	42-43	7.511082966698691
1104	44-45	6.961623287332163
1104	46-47	6.878446732566108
1104	48-49	5.720488221927365
1104	50-51	5.960662281899175
1104	52-53	6.127039471485681
1104	54-55	4.984168193140906
1104	56-57	6.2576169118236535
1104	58-59	4.131849052042899
1104	60-61	4.468298196848874
1104	62-63	4.074917574758235
1104	64-65	3.135830726871948
1104	66-67	3.3987211877426375
1104	68-69	4.363546768211993
1104	70-71	2.8303024873470584
1104	72-73	2.7860711205385122
1104	74-75	3.4052815576803894
1104	76-77	4.1509566633002635
1104	78-79	4.614263353629582
1104	80-81	3.4195754863338585
1104	82-83	3.245763072050309
1104	84-85	3.281535115834167
1104	86-87	0.8705554507184381
1104	88-89	0.5302173858071271
1104	90-91	2.4600946761078326
1104	92-93	2.6691579472741473
1104	94-95	2.622184385622262
1104	96-97	3.0206672081906483
1104	98-99	2.6938679194053243
1104	100	0.9195676398803805
1105	1	5.797021373387935
1105	2	-2.8197676250277013
1105	3	0.3679966800366472
1105	4	8.812055894813412
1105	5	8.873407945035975
1105	6	8.413971657853526
1105	7	5.670490537857564
1105	8	6.3323040912055575
1105	9	5.374413376492207
1105	10-11	5.301391356085535
1105	12-13	5.321625635001482
1105	14-15	6.255581765819127
1105	16-17	6.416736372765946
1105	18-19	6.416774387062109
1105	20-21	7.37045847571423
1105	22-23	2.4840409131180685
1105	24-25	4.251460982027009
1105	26-27	7.244042650696809
1105	28-29	6.22418875121588
1105	30-31	5.559429066590511
1105	32-33	7.437367872655596
1105	34-35	6.376487590584016
1105	36-37	6.4859175816102415
1105	38-39	4.461273639289878
1105	40-41	5.685626472649581
1105	42-43	5.952306245440504
1105	44-45	6.498567830663557
1105	46-47	5.847719352791987
1105	48-49	5.31865400442026
1105	50-51	6.232880548643564
1105	52-53	5.874065587360157
1105	54-55	5.570781940374491
1105	56-57	5.47073664994652
1105	58-59	2.363163775821132
1105	60-61	4.859033327739429
1105	62-63	-0.5774382294036471
1105	64-65	-0.2191031014611795
1105	66-67	1.128253634851358
1105	68-69	3.3163851189365836
1105	70-71	3.3212947262727788
1105	72-73	3.0822303614376665
1105	74-75	3.0166655365014705
1105	76-77	2.118719247261801
1105	78-79	3.6863081049021424
1105	80-81	2.1020382608814607
1105	82-83	1.3314061515427298
1105	84-85	1.6678565647150307
1105	86-87	0.057694100925257175
1105	88-89	1.8419391845640334
1105	90-91	1.7406752221036577
1105	92-93	1.9720347335072823
1105	94-95	1.3170711293460613
1105	96-97	2.3569891994184964
1105	98-99	2.1835572363788636
1105	100	2.3381970099079012
1106	1	6.310420820100152
1106	2	10.472802260688404
1106	3	6.906956924235814
1106	4	12.04551251319766
1106	5	10.189423584105743
1106	6	9.568274325363657
1106	7	6.852510398448068
1106	8	7.56550403329603
1106	9	7.038432325776956
1106	10-11	5.910412732255139
1106	12-13	8.105910322931717
1106	14-15	7.943156229045702
1106	16-17	7.773399797757897
1106	18-19	7.276926765007115
1106	20-21	8.221193386452775
1106	22-23	6.385581906315448
1106	24-25	5.146256135810727
1106	26-27	7.911559339027097
1106	28-29	6.559528329756201
1106	30-31	5.476362026169589
1106	32-33	7.541655031182582
1106	34-35	6.911361733524739
1106	36-37	6.1578510080324484
1106	38-39	7.164887835587091
1106	40-41	6.95368783751703
1106	42-43	6.367593845282858
1106	44-45	4.668962117273065
1106	46-47	6.298335043686581
1106	48-49	6.143518681111285
1106	50-51	6.701361381673536
1106	52-53	5.655274530955367
1106	54-55	5.477357233427828
1106	56-57	5.150781038509667
1106	58-59	5.058638466104817
1106	60-61	5.043440207923002
1106	62-63	5.118026887973304
1106	64-65	5.422365795072043
1106	66-67	3.2443963356804524
1106	68-69	3.5240012710791824
1106	70-71	3.1270917026680003
1106	72-73	3.0387531477532477
1106	74-75	3.1572818323688097
1106	76-77	3.654574547574814
1106	78-79	4.460988457241061
1106	80-81	3.953766830596525
1106	82-83	2.290387164141201
1106	84-85	2.975002446721568
1106	86-87	3.667016477258162
1106	88-89	3.577277119536806
1106	90-91	3.3585882549998374
1106	92-93	3.088798187069333
1106	94-95	1.60663512234796
1106	96-97	1.6128641228789569
1106	98-99	2.095165343083462
1106	100	3.1027842838692834
1107	1	6.14797301664294
1107	2	10.505124182595342
1107	3	6.398478483560645
1107	4	11.56727722845601
1107	5	9.566805844540458
1107	6	8.047137389286423
1107	7	3.823051468391
1107	8	6.49133972372492
1107	9	5.707432872214252
1107	10-11	4.579196237056127
1107	12-13	6.291115966308791
1107	14-15	6.304476152315782
1107	16-17	7.011883553809163
1107	18-19	6.340810508307019
1107	20-21	5.637464388512051
1107	22-23	5.864722043745989
1107	24-25	4.791595462282583
1107	26-27	7.250493827883002
1107	28-29	6.485732917334648
1107	30-31	5.185312526989591
1107	32-33	5.079910059739735
1107	34-35	6.902991228941698
1107	36-37	6.559757457413188
1107	38-39	6.534703957878161
1107	40-41	7.784175287650733
1107	42-43	6.313908676672131
1107	44-45	4.334617294549865
1107	46-47	3.5127983414304573
1107	48-49	4.330887334321812
1107	50-51	5.171449483859043
1107	52-53	4.471802015503876
1107	54-55	5.577149218228765
1107	56-57	5.440651859970899
1107	58-59	3.2106346167884503
1107	60-61	2.985233374650136
1107	62-63	0.8852334256779955
1107	64-65	2.3644625428865567
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2102	100	3.6653533904592877
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2103	3	7.87005590482795
2103	4	12.235263142178592
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2103	6	9.438267226692123
2103	7	6.70661217504756
2103	8	7.872003663875368
2103	9	6.724894386516471
2103	10-11	7.61659835527222
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2103	66-67	4.599434943761349
2103	68-69	5.49221841799271
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2103	78-79	3.726382192059802
2103	80-81	4.1568491993314645
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2103	86-87	3.0939605233077785
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2104	3	7.802219168387861
2104	4	12.352680171927819
2104	5	10.235355471114932
2104	6	9.275256624862404
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2207	14-15	8.375992346806665
2207	16-17	7.423617341004892
2207	18-19	4.384021396915195
2207	20-21	7.56796828611791
2207	22-23	6.392112904994324
2207	24-25	4.347836511003475
2207	26-27	4.0403078246228326
2207	28-29	5.212972071009926
2207	30-31	5.598857185777028
2207	32-33	6.955983188780348
2207	34-35	6.475500402326158
2207	36-37	6.896896583198032
2207	38-39	6.890791364185187
2207	40-41	4.193247042278443
2207	42-43	5.798670284428528
2207	44-45	4.20159426377635
2207	46-47	5.72826077810139
2207	48-49	2.593286749002889
2207	50-51	5.549896151408355
2207	52-53	3.0049908933967515
2207	54-55	3.347414816953428
2207	56-57	5.460752574772066
2207	58-59	3.801862065437227
2207	60-61	3.6267896659709216
2207	62-63	4.4549042974268644
2207	64-65	3.73592344233068
2207	66-67	3.7625977773575734
2207	68-69	3.479130359843058
2207	70-71	3.736866653557822
2207	72-73	3.969720160075937
2207	74-75	3.5081093748448815
2207	76-77	4.061710657013887
2207	78-79	4.315554550957049
2207	80-81	4.378775016335538
2207	82-83	3.971704431642813
2207	84-85	3.2797122843971707
2207	86-87	3.4567581763486963
2207	88-89	3.244944731052186
2207	90-91	1.5393685567421755
2207	92-93	-0.6172949171177677
2207	94-95	1.4086303919778658
2207	96-97	-0.9421467449234022
2207	98-99	0.7472082763485908
2207	100	2.1044741879940254
2208	1	1.3251030934824541
2208	2	8.827255485432108
2208	3	6.832685787985962
2208	4	12.001143469763331
2208	5	10.219846103671198
2208	6	9.258387586658973
2208	7	7.692172547140988
2208	8	7.957673360682499
2208	9	6.669204002996718
2208	10-11	6.83313753911138
2208	12-13	7.181912600940034
2208	14-15	8.021790314258734
2208	16-17	7.398649549337819
2208	18-19	6.589558488517472
2208	20-21	6.852526006101471
2208	22-23	6.0593041977757345
2208	24-25	6.348789640184901
2208	26-27	8.201741381869478
2208	28-29	7.27678462319167
2208	30-31	6.2174603700864495
2208	32-33	8.622680302314262
2208	34-35	6.859726117465765
2208	36-37	7.184762168751487
2208	38-39	7.491570700916778
2208	40-41	7.901015742890664
2208	42-43	7.267479602480957
2208	44-45	6.202164240675071
2208	46-47	4.847644717385098
2208	48-49	4.915824369436535
2208	50-51	6.3066926363433
2208	52-53	5.966513123794478
2208	54-55	6.6102020579968475
2208	56-57	6.777001834884331
2208	58-59	5.909865843899642
2208	60-61	5.445781823954583
2208	62-63	3.628371891343903
2208	64-65	3.7342575843509493
2208	66-67	2.968445377532195
2208	68-69	5.696921047752195
2208	70-71	5.474612181156271
2208	72-73	4.801203634930513
2208	74-75	2.3584514990978676
2208	76-77	3.627807569412955
2208	78-79	4.363704034891928
2208	80-81	3.7347533360222016
2208	82-83	1.4874166230343349
2208	84-85	2.1055546164598162
2208	86-87	1.3430612872187062
2208	88-89	0.9677655217810361
2208	90-91	2.205824362877305
2208	92-93	0.9317561816307212
2208	94-95	-0.8615469448418693
2208	96-97	1.578736796365952
2208	98-99	0.34930459984710893
2208	100	1.935354476807607
2209	1	5.324288070809509
2209	2	9.69782879097809
2209	3	5.589128569565354
2209	4	-3.5285348424286447
2209	5	0.5397539896993067
2209	6	4.440435208050854
2209	7	2.3492274162177154
2209	8	5.413461389148122
2209	9	5.226205813550443
2209	10-11	5.276251691515149
2209	12-13	7.432479561343062
2209	14-15	6.9011696936381135
2209	16-17	6.389036110762625
2209	18-19	5.433558772954914
2209	20-21	6.070201605197838
2209	22-23	5.5817583459129345
2209	24-25	5.332784017075451
2209	26-27	7.059691681896279
2209	28-29	6.369063240606895
2209	30-31	4.339032215155569
2209	32-33	6.500489805806833
2209	34-35	5.8675705739441
2209	36-37	6.474559069477348
2209	38-39	6.994343418990052
2209	40-41	7.461786349562907
2209	42-43	6.967784825737002
2209	44-45	6.629047954990487
2209	46-47	6.878988629603324
2209	48-49	5.374922265146452
2209	50-51	4.887549449440552
2209	52-53	5.122313186151921
2209	54-55	5.497040262321388
2209	56-57	5.21332723131902
2209	58-59	3.344883922087117
2209	60-61	4.446040552628613
2209	62-63	4.562961672381775
2209	64-65	5.2821705246136155
2209	66-67	4.72806948251149
2209	68-69	4.039465231279987
2209	70-71	3.897247384665789
2209	72-73	1.0293044133045157
2209	74-75	2.583137387171732
2209	76-77	3.518469269364271
2209	78-79	3.652159703675462
2209	80-81	3.853521284298349
2209	82-83	3.158131600306689
2209	84-85	3.179905470045643
2209	86-87	1.7066532945265607
2209	88-89	2.3246453524313573
2209	90-91	2.5508387761977254
2209	92-93	2.4318798025193686
2209	94-95	0.9279786463230622
2209	96-97	0.4761588085420634
2209	98-99	0.5306378456612286
2209	100	0.260421620453986
2210	1	2.0940323914012886
2210	2	-17.237306344157044
2210	3	-7.767606131237143
2210	4	-7.880792184516494
2210	5	-1.3434139239770815
2210	6	2.4443085729411678
2210	7	-8.55379255290395
2210	8	-8.54514806776642
2210	9	-3.6747324128115118
2210	10-11	-0.9308435393088921
2210	12-13	3.7130915292431617
2210	14-15	-5.425792862138003
2210	16-17	-1.413851522633479
2210	18-19	-0.11285713447794521
2210	20-21	2.4641353694705828
2210	22-23	2.5810712181071658
2210	24-25	2.1937560060135404
2210	26-27	4.2837386101804995
2210	28-29	3.4751430492290787
2210	30-31	2.740319943561939
2210	32-33	4.8536438072659145
2210	34-35	2.5126766970163246
2210	36-37	4.136612648480078
2210	38-39	3.287752081890236
2210	40-41	4.303684179596331
2210	42-43	2.319253243307113
2210	44-45	3.356359164505079
2210	46-47	3.0303840928631978
2210	48-49	3.057207092346534
2210	50-51	2.144547973388228
2210	52-53	2.415997548480348
2210	54-55	2.855071174589586
2210	56-57	2.8538304463985043
2210	58-59	1.8244931923743515
2210	60-61	2.7204989092903595
2210	62-63	1.903883950592185
2210	64-65	2.73290261263384
2210	66-67	2.67780565936631
2210	68-69	3.2979830711537588
2210	70-71	2.489115523991554
2210	72-73	1.1500548247328908
2210	74-75	0.4760141622519001
2210	76-77	-0.19596635269846985
2210	78-79	0.2690172391770673
2210	80-81	0.9497590474852657
2210	82-83	0.794559553683797
2210	84-85	-1.1885231280439719
2210	86-87	-0.9242386143314505
2210	88-89	-1.4310824092286083
2210	90-91	-1.7398841501184208
2210	92-93	-1.1161309200676257
2210	94-95	-0.24819498493531356
2210	96-97	-0.010009563219529838
2210	98-99	-0.24507261055770257
2210	100	-0.23290899830035983
2211	1	1.2206898770137755
2211	2	-17.237306344157044
2211	3	-7.964271494680776
2211	4	-19.308915222809024
2211	5	-21.318304068358753
2211	6	-13.642996490513159
2211	7	-11.436879525610433
2211	8	-18.901239371993192
2211	9	-16.114260407422673
2211	10-11	-10.055509924741434
2211	12-13	-22.612865784002285
2211	14-15	-18.02221101095765
2211	16-17	-15.88473150702288
2211	18-19	-19.32905423140241
2211	20-21	-8.455213784101268
2211	22-23	-4.035861696790967
2211	24-25	-1.8552261697793178
2211	26-27	-17.68752456155552
2211	28-29	-3.624837145056439
2211	30-31	-4.720431049823908
2211	32-33	-0.8007941468002677
2211	34-35	-5.991948691874082
2211	36-37	-6.910006002489887
2211	38-39	-2.7046971795660184
2211	40-41	-5.789719125349201
2211	42-43	-1.9133953481006465
2211	44-45	-0.028966041115204177
2211	46-47	0.8604696633765734
2211	48-49	0.5840438354681723
2211	50-51	-5.109297938387083
2211	52-53	-1.2270355662531145
2211	54-55	-0.5288208951461826
2211	56-57	-7.065418976685482
2211	58-59	-2.4404796002126723
2211	60-61	-1.170769063547386
2211	62-63	-0.6184642186602218
2211	64-65	-3.6132400928618544
2211	66-67	-2.8775671641985774
2211	68-69	0.09532451956566135
2211	70-71	-0.4931539745764191
2211	72-73	-1.296306960275551
2211	74-75	-1.848779024207964
2211	76-77	-1.771496842853054
2211	78-79	-1.2125983081567178
2211	80-81	-1.1611015383006205
2211	82-83	-1.752894259694198
2211	84-85	-1.782566170435686
2211	86-87	-2.1658411902675887
2211	88-89	-2.8441166634048294
2211	90-91	-2.573399513526958
2211	92-93	-2.3684556055685526
2211	94-95	-2.685011421642777
2211	96-97	-3.258666214020568
2211	98-99	-3.1562039719297488
2211	100	-2.593517596437149
2212	1	-21.287323540802102
2212	2	-17.237306344157044
2212	3	-20.17783839740161
2212	4	-19.308915222809024
2212	5	-21.318304068358753
2212	6	-22.105911138295493
2212	7	-22.67840021140991
2212	8	-23.011563635876904
2212	9	-24.493313705969076
2212	10-11	-11.567923733910277
2212	12-13	-22.612865784002285
2212	14-15	-24.033765241296823
2212	16-17	-24.462815741089223
2212	18-19	-19.012267052870914
2212	20-21	-24.197666262670033
2212	22-23	-19.150631543976953
2212	24-25	-25.770619386327954
2212	26-27	-17.600216976762376
2212	28-29	-18.561471043677386
2212	30-31	-6.145153220279866
2212	32-33	-17.7274406246236
2212	34-35	-14.115772769399243
2212	36-37	-18.1794411807729
2212	38-39	-24.298249173602546
2212	40-41	-10.674925362148802
2212	42-43	-14.377157616705443
2212	44-45	-17.82748107403855
2212	46-47	-17.64332118020648
2212	48-49	-18.41822212924794
2212	50-51	-9.560579929938829
2212	52-53	-2.783508885920149
2212	54-55	-24.31096774568662
2212	56-57	-24.22401010601832
2212	58-59	-13.234804516351325
2212	60-61	-6.684869107031048
2212	62-63	-3.355823362653261
2212	64-65	-18.041712108019016
2212	66-67	-18.40345454151253
2212	68-69	-15.28825123518648
2212	70-71	-10.24311297569457
2212	72-73	-18.420492883992782
2212	74-75	-6.83458533555099
2212	76-77	-11.611262962867961
2212	78-79	-4.845489195223433
2212	80-81	-2.5590725595454984
2212	82-83	-3.2276516829765924
2212	84-85	-5.611111797221625
2212	86-87	-5.0391687775455125
2212	88-89	-3.664090883804878
2212	90-91	-4.324647960539011
2212	92-93	-5.684123700984134
2212	94-95	-4.026772354677938
2212	96-97	-5.717180781047528
2212	98-99	-4.046397940124557
2212	100	-3.869082884050517
2213	1	-21.287323540802102
2213	2	-17.237306344157044
2213	3	-20.17783839740161
2213	4	-19.308915222809024
2213	5	-21.318304068358753
2213	6	-22.105911138295493
2213	7	-10.890442096226666
2213	8	-10.698998190850725
2213	9	-4.216873915393158
2213	10-11	-17.980849126580434
2213	12-13	-16.575169448923752
2213	14-15	-24.033765241296823
2213	16-17	-18.39449113375938
2213	18-19	-18.9223717649128
2213	20-21	-18.169394011361135
2213	22-23	-20.417293847641872
2213	24-25	-7.846012056485023
2213	26-27	-23.798966976762376
2213	28-29	-24.715221043677385
2213	30-31	-19.977206012600984
2213	32-33	-23.9299406246236
2213	34-35	-18.429699470969922
2213	36-37	-24.34235784743957
2213	38-39	-12.048510953707257
2213	40-41	-17.72575433248039
2213	42-43	-23.907990950038776
2213	44-45	-17.78549590824448
2213	46-47	-17.626807218600895
2213	48-49	-6.585615235705184
2213	50-51	-24.158496596605495
2213	52-53	-18.512673371085945
2213	54-55	-18.420653609560965
2213	56-57	-18.335004870416224
2213	58-59	-18.737271793838232
2213	60-61	-20.26108856601883
2213	62-63	-18.33900180942464
2213	64-65	-18.453690729310466
2213	66-67	-9.13565785739386
2213	68-69	-23.62700123518648
2213	70-71	-18.35525957255321
2213	72-73	-9.906910859559972
2213	74-75	-4.864890745673154
2213	76-77	-3.600935737737071
2213	78-79	-4.900927676898828
2213	80-81	-3.6657152297025632
2213	82-83	-3.7328305660481433
2213	84-85	-3.5429464394554806
2213	86-87	-3.751631692030678
2213	88-89	-3.1499852991626476
2213	90-91	-3.2677478732789744
2213	92-93	-4.266388098889895
2213	94-95	-4.051469126056645
2213	96-97	-3.680097447714193
2213	98-99	-3.9693713258139134
2213	100	-3.5334371597922285
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	584.0
3	551.0
4	2607.0
5	9752.0
6	15470.0
7	18240.0
8	16238.0
9	16742.0
10	19138.0
11	23334.0
12	29097.0
13	40594.0
14	30425.0
15	33081.0
16	38849.0
17	41675.0
18	42441.0
19	42785.0
20	42657.0
21	41334.0
22	42235.0
23	46842.0
24	51457.0
25	57244.0
26	63339.0
27	68687.0
28	75034.0
29	79190.0
30	82663.0
31	89693.0
32	101221.0
33	124480.0
34	152556.0
35	185972.0
36	212773.0
37	200314.0
38	71711.0
39	216.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.95897874116869	22.069946832097518	14.471655299910383	23.499419126823405
2	14.113000060933864	21.75398728513345	41.74005613084293	22.39295652308975
3	16.965364386559635	27.773868156688646	30.56751525993163	24.693252196820094
4	11.34680367364252	18.27622855890077	41.58548558519486	28.791482182261852
5	12.321752914964991	38.23441564772501	36.80508591299288	12.638745524317125
6	28.562948967309442	39.83957018353656	19.63336767871159	11.964113170442404
7	24.54565786543802	33.23733300897256	25.49843492659212	16.718574198997295
8	22.497726317266302	37.71576520477471	22.713312443281435	17.07319603467755
9	23.583883088654844	17.70248711406604	23.367288715528574	35.346341081750545
10-11	22.128891615522086	28.314520372557244	30.341058165390777	19.21552984652989
12-13	22.92106002166782	25.852514774597203	30.464166661020087	20.762258542714893
14-15	20.387034663265354	27.402443540875076	27.632583732921244	24.57793806293833
16-17	19.916957839511785	31.162426044711506	28.444929916327986	20.475686199448724
18-19	19.78983483286411	30.270777626616596	30.05281008854795	19.886577451971345
20-21	21.354825340655918	29.622160996322766	28.96109294361291	20.061920719408405
22-23	20.341574090063126	29.68197778161515	29.55174696367927	20.424701164642453
24-25	20.640423003688873	29.339844141465722	29.14022495931739	20.879507895528015
26-27	20.58442888765634	29.392885517696264	29.251450329041056	20.77123526560634
28-29	20.302559712559834	29.392951446243625	29.637516729162392	20.666972112034145
30-31	20.932949552038586	29.738301510246966	28.924809910837713	20.403939026876742
32-33	20.153341231904783	29.550932786623434	29.561629284634556	20.734096696837227
34-35	20.307800788493605	28.93416526420769	29.452685514157046	21.305348433141663
36-37	20.765185333320048	29.6529667206027	28.99773993534966	20.584108010727594
38-39	20.45193707244288	29.29680773385949	29.31010764025509	20.941147553442534
40-41	20.75962675259242	29.352819784661914	28.95392112833875	20.933632334406912
42-43	20.321361811596457	29.523686057350034	29.48945342950336	20.66549870155015
44-45	20.829997311878554	29.047951357066985	29.29152315138564	20.830528179668825
46-47	20.750981218390564	29.54571196284952	29.260681098503866	20.442625720256054
48-49	20.520485830035664	29.19757729194356	29.39714200199988	20.884794876020894
50-51	20.88681091807387	29.2878355622839	29.093118382828177	20.732235136814058
52-53	20.2974328833414	29.276378523237124	29.502060668698704	20.92412792472277
54-55	20.76899459276542	29.273542762874914	29.041131959432192	20.91633068492747
56-57	20.886958456934572	29.3028830811959	29.016892966764267	20.79326549510526
58-59	20.47835352162727	28.856684859517443	29.491556710369643	21.173404908485644
60-61	20.94486529655125	29.33805779339083	28.89084955657507	20.82622735348285
62-63	20.46272523587522	29.421838641620283	29.411680916670463	20.70375520583404
64-65	20.550926535485576	28.847539864084965	29.381030848586093	21.220502751843366
66-67	20.645776204583946	29.203475681851547	29.089013634946888	21.06173447861762
68-69	21.065577068536925	29.107045540417925	29.049976870165562	20.777400520879585
70-71	21.151182727854955	29.198470053222696	28.80940035052202	20.84094686840033
72-73	20.67905719917029	29.325109711427093	29.383515334345788	20.61231775505683
74-75	20.998673018118698	28.866314157886443	29.001589231264962	21.1334235927299
76-77	20.968404788081454	29.397626987700654	28.90791606000416	20.726052164213733
78-79	20.930881707592935	29.09439685712767	29.094488904700615	20.88023253057878
80-81	21.15675380523885	29.096790198053966	28.857382017847172	20.889073978860015
82-83	20.57999320845707	29.279777383250416	29.413579895209686	20.726649513082837
84-85	20.803085299455535	28.898974619122008	28.99848637739657	21.29945370402589
86-87	21.011437387101147	29.282497370428253	28.969880607896986	20.73618463457362
88-89	20.777785166487366	28.861620501907957	29.23961372636005	21.120980605244625
90-91	21.087066350970012	28.865259280281503	28.907795298079936	21.139879070668545
92-93	20.701430128237565	29.152303404731217	29.25068413862517	20.895582328406054
94-95	20.686207830892585	28.711886182735373	29.134350126114068	21.467555860257974
96-97	20.861120470111295	28.955912970846754	29.116145116488862	21.066821442553092
98-99	20.82745408736688	28.819995584319063	29.108173280042305	21.24437704827175
100	21.043690059093166	28.646580713796748	28.887423180928472	21.42230604618161
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	256.0
1	298.5
2	291.0
3	258.5
4	298.5
5	414.5
6	609.0
7	915.5
8	1388.0
9	2059.5
10	2808.5
11	3512.0
12	4317.0
13	5164.0
14	5928.5
15	6652.0
16	7345.5
17	8000.0
18	8692.0
19	9495.5
20	10475.5
21	11789.5
22	13268.5
23	15221.0
24	17755.5
25	20997.5
26	25546.5
27	31125.0
28	37709.0
29	45142.0
30	52474.5
31	59506.5
32	66353.5
33	72783.5
34	78204.5
35	83108.0
36	86985.5
37	89390.5
38	91190.5
39	91259.5
40	89997.5
41	87766.0
42	84117.0
43	79744.0
44	75150.0
45	70303.5
46	65576.5
47	61369.5
48	57528.5
49	54203.5
50	51558.5
51	47438.5
52	42057.0
53	37909.0
54	34357.5
55	30969.5
56	28124.5
57	25225.5
58	22059.5
59	19145.0
60	16300.0
61	13372.0
62	10728.0
63	8617.0
64	6860.0
65	5426.5
66	4189.5
67	3158.0
68	2351.5
69	1757.5
70	1329.0
71	980.0
72	716.5
73	520.0
74	390.0
75	296.0
76	220.5
77	149.5
78	109.5
79	80.0
80	56.0
81	44.0
82	31.5
83	18.0
84	14.5
85	11.0
86	7.5
87	6.5
88	5.0
89	2.5
90	0.5
91	0.0
92	0.5
93	1.0
94	0.5
95	0.0
96	1.0
97	1.5
98	0.5
99	1.0
100	2.5
>>END_MODULE
>>Per base N content	fail
#Base	N-Count
1	7.702893559712032
2	24.297661789572366
3	5.854774353174106
4	24.089360584039316
5	12.332100681026455
6	9.715627700713767
7	7.793838788614978
8	8.455599869936112
9	5.2069422278460635
10-11	6.749935895145713
12-13	9.897947785409057
14-15	7.318038314578235
16-17	7.735658263013964
18-19	6.623670813545999
20-21	7.793137818427014
22-23	5.719057480007652
24-25	5.2338052144041685
26-27	9.494799479563554
28-29	6.229409000728556
30-31	4.2922665803191995
32-33	8.677038613508103
34-35	7.13112800574886
36-37	6.94351672673152
38-39	5.981265554189292
40-41	8.463559273360737
42-43	6.335752961825164
44-45	6.292541541528414
46-47	5.769979572372006
48-49	3.8256013306675363
50-51	6.378331247758591
52-53	4.4351966628392185
54-55	4.847457581128254
56-57	4.694487796561266
58-59	3.4720410126351005
60-61	4.0729307473111005
62-63	3.1653778613716135
64-65	4.341605836775248
66-67	2.351189682080624
68-69	5.0252552775140975
70-71	2.4489863292723792
72-73	1.508148665375374
74-75	0.8434932555361947
76-77	1.0720773726371087
78-79	1.73802166314448
80-81	2.1032723549568315
82-83	0.7830967596635523
84-85	1.2236226048866214
86-87	0.5084521176309378
88-89	0.7090652630379325
90-91	0.32590591352017734
92-93	0.3408750188244413
94-95	0.5257276409730189
96-97	0.538706895421127
98-99	0.4511082338671711
100	0.4423800244299416
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	2211221.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	97.61448771763071
#Duplication Level	Percentage of deduplicated	Percentage of total
1	98.31073138287964	95.96551681085397
2	1.483147789625719	2.8955342338770182
3	0.12889927859227931	0.37747311140872525
4	0.030322686157235886	0.11839733901844293
5	0.013410625475590153	0.06545356678863702
6	0.010475240425618865	0.061352113671948094
7	0.004506348951865511	0.030791946108926382
8	0.004030521820821581	0.031474985821938466
9	0.0025766118152955432	0.022636317815754207
>10	0.01023584938760546	0.16488179914579765
>50	0.001013150228247294	0.07431167097779323
>100	5.576245744672335E-4	0.11700722444621758
>500	9.289006564370022E-5	0.0751688800647765
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	4.5223883094453246E-5	0.0
5	0.0	0.0	0.0	4.5223883094453246E-5	0.0
6	0.0	0.0	0.0	4.5223883094453246E-5	0.0
7	0.0	0.0	0.0	4.5223883094453246E-5	0.0
8	0.0	0.0	0.0	4.5223883094453246E-5	0.0
9	0.0	0.0	0.0	1.3567164928335974E-4	0.0
10-11	0.0	0.0	0.0	1.3567164928335974E-4	4.5223883094453246E-5
12-13	0.0	0.0	0.0	1.5828359083058636E-4	4.5223883094453246E-5
14-15	0.0	0.0	0.0	1.8089553237781299E-4	4.5223883094453246E-5
16-17	0.0	0.0	0.0	1.8089553237781299E-4	4.5223883094453246E-5
18-19	4.5223883094453246E-5	0.0	0.0	1.8089553237781299E-4	4.5223883094453246E-5
20-21	4.5223883094453246E-5	0.0	0.0	1.8089553237781299E-4	4.5223883094453246E-5
22-23	4.5223883094453246E-5	0.0	0.0	2.2611941547226623E-4	4.5223883094453246E-5
24-25	4.5223883094453246E-5	0.0	0.0	2.4873135701949283E-4	4.5223883094453246E-5
26-27	4.5223883094453246E-5	0.0	0.0	2.713432985667195E-4	4.5223883094453246E-5
28-29	4.5223883094453246E-5	0.0	0.0	3.6179106475562597E-4	4.5223883094453246E-5
30-31	4.5223883094453246E-5	0.0	0.0	0.001266268726644691	4.5223883094453246E-5
32-33	9.044776618890649E-5	0.0	0.0	0.0042510450108786055	4.5223883094453246E-5
34-35	9.044776618890649E-5	0.0	0.0	0.008796045261871156	4.5223883094453246E-5
36-37	1.1305970773613312E-4	0.0	0.0	0.01632582179709762	4.5223883094453246E-5
38-39	1.3567164928335974E-4	0.0	0.0	0.02939552401139461	4.5223883094453246E-5
40-41	1.8089553237781299E-4	0.0	0.0	0.04438724125720586	4.5223883094453246E-5
42-43	1.8089553237781299E-4	0.0	0.0	0.06136880935917305	4.5223883094453246E-5
44-45	1.8089553237781299E-4	0.0	0.0	0.08090552685597685	4.5223883094453246E-5
46-47	1.8089553237781299E-4	0.0	0.0	0.1063439610966068	4.5223883094453246E-5
48-49	1.8089553237781299E-4	0.0	0.0	0.13293560435614532	4.5223883094453246E-5
50-51	1.8089553237781299E-4	0.0	0.0	0.16300948661395673	4.5223883094453246E-5
52-53	1.8089553237781299E-4	2.2611941547226623E-5	0.0	0.19550284661732137	4.5223883094453246E-5
54-55	1.8089553237781299E-4	4.5223883094453246E-5	0.0	0.2308453112556366	4.5223883094453246E-5
56-57	1.8089553237781299E-4	4.5223883094453246E-5	0.0	0.2752551644543897	4.5223883094453246E-5
58-59	1.8089553237781299E-4	4.5223883094453246E-5	0.0	0.32459442091043816	4.5223883094453246E-5
60-61	1.8089553237781299E-4	4.5223883094453246E-5	0.0	0.3761044237550204	4.5223883094453246E-5
62-63	1.8089553237781299E-4	4.5223883094453246E-5	0.0	0.43078009841621434	4.5223883094453246E-5
64-65	1.8089553237781299E-4	4.5223883094453246E-5	0.0	0.4957215945398492	4.5223883094453246E-5
66-67	1.8089553237781299E-4	4.5223883094453246E-5	0.0	0.5723534644434003	4.5223883094453246E-5
68-69	1.8089553237781299E-4	4.5223883094453246E-5	0.0	0.6607435439515091	4.5223883094453246E-5
70-71	1.8089553237781299E-4	4.5223883094453246E-5	0.0	0.7628364600372374	4.5223883094453246E-5
72-73	1.8089553237781299E-4	6.783582464167987E-5	0.0	0.8693839286077691	4.5223883094453246E-5
74-75	2.2611941547226623E-4	9.044776618890649E-5	0.0	0.9809286362602381	4.5223883094453246E-5
76-77	2.2611941547226623E-4	9.044776618890649E-5	0.0	1.1069675984444793	4.5223883094453246E-5
78-79	2.2611941547226623E-4	9.044776618890649E-5	0.0	1.2519553676452964	4.5223883094453246E-5
80-81	2.2611941547226623E-4	9.044776618890649E-5	0.0	1.4193741828609623	4.5223883094453246E-5
82-83	2.2611941547226623E-4	1.3567164928335974E-4	0.0	1.5997948644662836	4.5223883094453246E-5
84-85	2.2611941547226623E-4	1.3567164928335974E-4	0.0	1.7843535313747472	4.5223883094453246E-5
86-87	2.2611941547226623E-4	1.8089553237781299E-4	0.0	1.979788542167427	4.5223883094453246E-5
88	2.2611941547226623E-4	1.8089553237781299E-4	0.0	2.1476822081555844	4.5223883094453246E-5
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGACCG	115	0.0027520352	21.758104	5
AACGCCG	140	3.3556772E-4	21.50097	6
GTATACG	150	0.0037468558	20.630188	1
CCGACGT	55	0.0042648907	20.174986	72-73
GTATAAG	830	0.0	20.133076	1
GTACATG	5445	0.0	19.095713	1
TAGCACG	135	0.006960854	18.53468	5
GTCTCGC	305	2.4357305E-6	18.26279	1
GGACCGT	200	1.8189335E-4	17.559126	6
TACATGG	5620	0.0	17.395796	2
CTGTCGC	405	2.3283064E-10	17.358704	9
TCTGTCG	390	2.6575435E-9	16.731085	8
TCTAGAC	605	0.0	16.711996	3
GTATTAG	980	0.0	16.419947	1
ACATGGG	5840	0.0	16.351112	3
TGGACCG	190	0.0026528616	15.803253	5
ATACCGT	225	4.4657796E-4	15.608112	6
TAGACAG	1365	0.0	15.398043	5
GTATCAA	3745	0.0	14.873568	1
CCTATAC	650	1.0913936E-11	14.690845	3
>>END_MODULE