FastQCFastQC Report
Wed 25 May 2016
SRR1295091_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295091_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2211221
Sequences flagged as poor quality0
Sequence length100
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT24390.11030105086737146No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA56950.023.9973751
CAACGCA61200.021.9049155
AACGCAG62200.021.5921526
ATCAACG62750.021.5872523
TCAACGC61900.021.5812114
TATCAAC65550.021.1330072
ACGCAGA68350.019.3705717
CGCAGAG69050.019.17428
TATACCG1250.00638320518.815175
TACCGGT1550.001041208418.1740367
ACCGCTC1650.00158325217.0725788
GAGTACT27700.017.03405812-13
GCAGAGT78800.016.3251119
GTACATG71300.015.8219921
ACATGGG71250.015.381183
TACATGG73500.015.2669012
GGTATCA44000.014.7959691
TCTATAC9950.014.6540343
GCACCGT1950.00481584214.4489726
TAGGGTG6900.014.3158895
Produced by FastQC (version 0.11.5)