##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1295091_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2211221 Sequences flagged as poor quality 0 Sequence length 100 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.42815937439089 33.0 33.0 33.0 27.0 33.0 2 30.138935909165117 33.0 33.0 33.0 27.0 33.0 3 30.9317137454827 33.0 33.0 33.0 27.0 33.0 4 35.21814689712154 37.0 37.0 37.0 33.0 37.0 5 35.47689579648529 37.0 37.0 37.0 33.0 37.0 6 35.21701177765588 37.0 37.0 37.0 33.0 37.0 7 35.1413060928781 37.0 37.0 37.0 33.0 37.0 8 35.1257110890318 37.0 37.0 37.0 33.0 37.0 9 35.12140894103303 37.0 37.0 37.0 33.0 37.0 10-11 35.089783653465666 37.0 37.0 37.0 33.0 37.0 12-13 35.00585558838307 37.0 37.0 37.0 33.0 37.0 14-15 36.47397297692089 40.0 37.0 40.0 33.0 40.0 16-17 36.47438767992887 40.0 37.0 40.0 33.0 40.0 18-19 36.51871793909338 40.0 37.0 40.0 33.0 40.0 20-21 36.522596339307555 40.0 37.0 40.0 33.0 40.0 22-23 36.47818377267582 40.0 37.0 40.0 33.0 40.0 24-25 36.444013284967895 40.0 37.0 40.0 33.0 40.0 26-27 36.42185697404285 40.0 37.0 40.0 33.0 40.0 28-29 36.37039920478324 40.0 37.0 40.0 33.0 40.0 30-31 36.28674270911863 37.0 37.0 40.0 33.0 40.0 32-33 36.243390868664875 37.0 37.0 40.0 30.0 40.0 34-35 36.146609045409754 37.0 37.0 40.0 27.0 40.0 36-37 36.13836292256631 37.0 37.0 40.0 27.0 40.0 38-39 36.084036828521434 37.0 37.0 40.0 27.0 40.0 40-41 36.02984550164818 37.0 37.0 40.0 27.0 40.0 42-43 35.94595632910505 37.0 37.0 40.0 27.0 40.0 44-45 35.84848687670748 37.0 37.0 40.0 27.0 40.0 46-47 35.74401699332631 37.0 37.0 40.0 27.0 40.0 48-49 35.649758210509034 37.0 37.0 40.0 27.0 40.0 50-51 35.55861263980398 37.0 37.0 40.0 27.0 40.0 52-53 35.47784956817975 37.0 37.0 40.0 27.0 40.0 54-55 35.36907030097851 37.0 35.0 40.0 27.0 40.0 56-57 35.251487978813515 37.0 33.0 40.0 27.0 40.0 58-59 35.13767054491613 37.0 33.0 40.0 27.0 40.0 60-61 34.98753991572981 37.0 33.0 40.0 27.0 40.0 62-63 34.82206889315903 37.0 33.0 40.0 27.0 40.0 64-65 34.65343016369689 37.0 33.0 40.0 27.0 40.0 66-67 34.44897298822687 37.0 33.0 40.0 27.0 40.0 68-69 34.23694239517443 37.0 33.0 40.0 27.0 40.0 70-71 34.01689993899298 37.0 33.0 37.0 27.0 40.0 72-73 33.80767707072246 37.0 33.0 37.0 27.0 40.0 74-75 33.572567599529854 37.0 33.0 37.0 24.5 40.0 76-77 33.336997070849094 37.0 33.0 37.0 22.0 40.0 78-79 33.09680036504719 37.0 33.0 37.0 22.0 37.0 80-81 32.86859002334005 37.0 33.0 37.0 22.0 37.0 82-83 32.64220107352454 37.0 33.0 37.0 22.0 37.0 84-85 32.46966268862316 37.0 33.0 37.0 22.0 37.0 86-87 32.47429836275976 37.0 33.0 37.0 22.0 37.0 88-89 32.475486394168655 37.0 33.0 37.0 22.0 37.0 90-91 32.46678102279239 37.0 33.0 37.0 22.0 37.0 92-93 32.42465542792873 37.0 33.0 37.0 22.0 37.0 94-95 32.435111641939 35.0 33.0 37.0 22.0 37.0 96-97 32.39337542470879 33.0 33.0 37.0 22.0 37.0 98-99 32.3142395083983 33.0 33.0 37.0 22.0 37.0 100 31.78769602857426 33.0 33.0 37.0 22.0 37.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.5817964840860519 1101 2 4.0057855461723015 1101 3 2.2542766189253776 1101 4 0.9750432095218571 1101 5 0.4543351726071023 1101 6 0.418971085269483 1101 7 0.48079556973973325 1101 8 0.49266960212018773 1101 9 0.5490284028647423 1101 10-11 0.5004126955213763 1101 12-13 0.5032759610443449 1101 14-15 0.7944155907581703 1101 16-17 0.8461366103457237 1101 18-19 0.8385449632187587 1101 20-21 0.7876201655285726 1101 22-23 0.8494257367719129 1101 24-25 0.8203477669938977 1101 26-27 0.8588573425790926 1101 28-29 0.8055422106666796 1101 30-31 0.6246390734113945 1101 32-33 0.805851933331823 1101 34-35 1.032279151272526 1101 36-37 0.8479190662286484 1101 38-39 0.8692403494063825 1101 40-41 0.8153654007481563 1101 42-43 0.962841038175533 1101 44-45 1.001006436214027 1101 46-47 0.8846672394167001 1101 48-49 0.8987122074554392 1101 50-51 0.8110819395353488 1101 52-53 0.768790960024063 1101 54-55 0.7683711518833789 1101 56-57 0.7897237865019022 1101 58-59 0.6834223084658504 1101 60-61 0.6874594096832425 1101 62-63 0.7856919593073215 1101 64-65 0.790510696543322 1101 66-67 0.8417916782987191 1101 68-69 0.7974287614223599 1101 70-71 0.8046479091193461 1101 72-73 0.7774094386919117 1101 74-75 0.7053342847556721 1101 76-77 0.7910041569263413 1101 78-79 0.7425702912479508 1101 80-81 0.7658482423531865 1101 82-83 0.7339127323601673 1101 84-85 0.7189874886212451 1101 86-87 0.7308888331456913 1101 88-89 0.654019539702432 1101 90-91 0.5794321191913596 1101 92-93 0.6729052443713783 1101 94-95 0.6160169028179041 1101 96-97 0.6278837111521227 1101 98-99 0.6418120289365348 1101 100 0.7843623760236866 1102 1 0.4862220642234085 1102 2 4.010309727406593 1102 3 2.246491754193759 1102 4 0.9093791585231799 1102 5 0.4375882213667168 1102 6 0.39060016026794386 1102 7 0.37003863513827895 1102 8 0.36575201620370734 1102 9 0.48948532308686765 1102 10-11 0.5237947645010621 1102 12-13 0.5571509327992388 1102 14-15 0.7757141637811813 1102 16-17 0.8095425079238368 1102 18-19 0.7920492393566079 1102 20-21 0.7710844534437697 1102 22-23 0.7670529938126478 1102 24-25 0.8240989738302389 1102 26-27 0.7192574473427555 1102 28-29 0.6397661207479572 1102 30-31 0.6472739309533466 1102 32-33 0.8317653629381709 1102 34-35 0.9394825091876626 1102 36-37 0.803684662662576 1102 38-39 0.7229217137990602 1102 40-41 0.7258065893022092 1102 42-43 0.8127998687939169 1102 44-45 0.8275058233808181 1102 46-47 0.8573175033800524 1102 48-49 0.8452622817486244 1102 50-51 0.7465762802432252 1102 52-53 0.7699117771806527 1102 54-55 0.7582719002931029 1102 56-57 0.8526447241709434 1102 58-59 0.6884656861012033 1102 60-61 0.7005029139936241 1102 62-63 0.7343636690092552 1102 64-65 0.673150655322587 1102 66-67 0.7371670129604269 1102 68-69 0.6476877604842812 1102 70-71 0.595073674133765 1102 72-73 0.6947409780330602 1102 74-75 0.6942063977634874 1102 76-77 0.7469658428681569 1102 78-79 0.6855665320811326 1102 80-81 0.6991220897816888 1102 82-83 0.7321850733830217 1102 84-85 0.5848020090022317 1102 86-87 0.6193856286100399 1102 88-89 0.5403066263823817 1102 90-91 0.6064980347093822 1102 92-93 0.6705500304960097 1102 94-95 0.6998259522074761 1102 96-97 0.6366306213087256 1102 98-99 0.7218834804953147 1102 100 0.8326137471951895 1103 1 0.40659839654774643 1103 2 3.944404942362983 1103 3 2.2145144800481944 1103 4 1.040015922085523 1103 5 0.4088588000412514 1103 6 0.4555750090415529 1103 7 0.42193950901543076 1103 8 0.43121278078135816 1103 9 0.3738433217307886 1103 10-11 0.44671562508389684 1103 12-13 0.5386195642345584 1103 14-15 0.7113923301046228 1103 16-17 0.7075111011449948 1103 18-19 0.7484372441779001 1103 20-21 0.7019187561545905 1103 22-23 0.5713272210627807 1103 24-25 0.6377854533106486 1103 26-27 0.6408503659437983 1103 28-29 0.7043198687855394 1103 30-31 0.7075365621348411 1103 32-33 0.6891401344420487 1103 34-35 0.8614391196409485 1103 36-37 0.7326016839496177 1103 38-39 0.6803444370591407 1103 40-41 0.7037197264181145 1103 42-43 0.7677643524154618 1103 44-45 0.7411772363160054 1103 46-47 0.7846165144397261 1103 48-49 0.6399657907198346 1103 50-51 0.6862426986931567 1103 52-53 0.6828940643926558 1103 54-55 0.5907255191738372 1103 56-57 0.4827718633749569 1103 58-59 0.5978131144987415 1103 60-61 0.6438611619060381 1103 62-63 0.7053139411711911 1103 64-65 0.6227076603282882 1103 66-67 0.6319062907338804 1103 68-69 0.6606300017130806 1103 70-71 0.7018967944704926 1103 72-73 0.6021258990770022 1103 74-75 0.6093898116971346 1103 76-77 0.5264861482588756 1103 78-79 0.6107692424115001 1103 80-81 0.5931384100317558 1103 82-83 0.6008656699866606 1103 84-85 0.6028586367745916 1103 86-87 0.6250254159990902 1103 88-89 0.6233037452452379 1103 90-91 0.6079101617795217 1103 92-93 0.6294884359349098 1103 94-95 0.6417987177836011 1103 96-97 0.6166932609744578 1103 98-99 0.576972501953442 1103 100 0.6328710617005129 1104 1 0.3073727731099005 1104 2 3.5216274193869666 1104 3 2.095621532232908 1104 4 0.8648366090764199 1104 5 0.26392711137868474 1104 6 0.2135681462216823 1104 7 0.2797672876107242 1104 8 0.2674008187517103 1104 9 0.3644692425527225 1104 10-11 0.40335200554791584 1104 12-13 0.3249244291102613 1104 14-15 0.4022381919867897 1104 16-17 0.4660979055406642 1104 18-19 0.4135162929927887 1104 20-21 0.3787802083241232 1104 22-23 0.3716983067080335 1104 24-25 0.48621699929615403 1104 26-27 0.4395961952805649 1104 28-29 0.5118856729810659 1104 30-31 0.3979133530719636 1104 32-33 0.5605491302927845 1104 34-35 0.6430673727045644 1104 36-37 0.5023239667394677 1104 38-39 0.530880111513433 1104 40-41 0.5794208042116651 1104 42-43 0.5809849207534796 1104 44-45 0.6145650930489097 1104 46-47 0.7349318271423471 1104 48-49 0.5944753074760669 1104 50-51 0.5788921810994836 1104 52-53 0.6000238496681689 1104 54-55 0.5778497208894748 1104 56-57 0.506293079291332 1104 58-59 0.5953307665321503 1104 60-61 0.5476499268311841 1104 62-63 0.5730116777796823 1104 64-65 0.49370576596372473 1104 66-67 0.40126745020958765 1104 68-69 0.5950436258856158 1104 70-71 0.5116270113217141 1104 72-73 0.4473271166833115 1104 74-75 0.4060628406503781 1104 76-77 0.47164633502438136 1104 78-79 0.3568714020443835 1104 80-81 0.40396609446377596 1104 82-83 0.43918280938517995 1104 84-85 0.5157454804467321 1104 86-87 0.5154604055677297 1104 88-89 0.5041959315195328 1104 90-91 0.48640798725237033 1104 92-93 0.5370921113100664 1104 94-95 0.49855315142373513 1104 96-97 0.34949660366325475 1104 98-99 0.4929226505559825 1104 100 0.38766801811437546 1105 1 0.44599461785071526 1105 2 3.726024484240643 1105 3 2.237954897455058 1105 4 0.9387613483853414 1105 5 0.38026738592362364 1105 6 0.3367617306658204 1105 7 0.3256269040273736 1105 8 0.3229493307116229 1105 9 0.3879004105405244 1105 10-11 0.3968874072918851 1105 12-13 0.3758309843249137 1105 14-15 0.4747205550406264 1105 16-17 0.49682146221308443 1105 18-19 0.5452849167437464 1105 20-21 0.4257118046771353 1105 22-23 0.41329640945379964 1105 24-25 0.4781541222538621 1105 26-27 0.5455459462099341 1105 28-29 0.5009280411276222 1105 30-31 0.43860987063528967 1105 32-33 0.46937193326292004 1105 34-35 0.6431855776747426 1105 36-37 0.47893752015069424 1105 38-39 0.567335786827897 1105 40-41 0.4406024698250448 1105 42-43 0.5495902763677236 1105 44-45 0.5675628582236811 1105 46-47 0.5000848685709016 1105 48-49 0.3780769484580091 1105 50-51 0.41504906767925576 1105 52-53 0.3107657518942091 1105 54-55 0.39456778011945204 1105 56-57 0.30751348448321636 1105 58-59 0.3444422954776556 1105 60-61 0.4087198334016975 1105 62-63 0.40792089956332944 1105 64-65 0.3016358035581561 1105 66-67 0.4485997165122768 1105 68-69 0.42489483965817243 1105 70-71 0.3293850875796167 1105 72-73 0.32442968925733595 1105 74-75 0.39599083475248875 1105 76-77 0.29183581505765943 1105 78-79 0.3900769963624384 1105 80-81 0.436537676264102 1105 82-83 0.3988790043854209 1105 84-85 0.3098992377733083 1105 86-87 0.3988992357626202 1105 88-89 0.3774031635476547 1105 90-91 0.3805635049839182 1105 92-93 0.41912768536034406 1105 94-95 0.4128590824236795 1105 96-97 0.41208100085613353 1105 98-99 0.4363698186689646 1105 100 0.3076155580462263 1106 1 0.4526371660378423 1106 2 3.918299856639827 1106 3 2.225635571863279 1106 4 0.9150722945801846 1106 5 0.40021994626965096 1106 6 0.2802214333619375 1106 7 0.27713982544071314 1106 8 0.28053741981256763 1106 9 0.2562114840244334 1106 10-11 0.3590268234337515 1106 12-13 0.36582585429599845 1106 14-15 0.43062958252771466 1106 16-17 0.3568926671544119 1106 18-19 0.3823736707901517 1106 20-21 0.3289805108362813 1106 22-23 0.2684803176685975 1106 24-25 0.2693893524389992 1106 26-27 0.2774594032864144 1106 28-29 0.3072865406273806 1106 30-31 0.24930026342345712 1106 32-33 0.25862893757081906 1106 34-35 0.3550413035668427 1106 36-37 0.33621155671411884 1106 38-39 0.1742737662818996 1106 40-41 0.28833022020420174 1106 42-43 0.3396217244591426 1106 44-45 0.2789458835979204 1106 46-47 0.29857933446914586 1106 48-49 0.26941829365859604 1106 50-51 0.23525812072907826 1106 52-53 0.21991877957293582 1106 54-55 0.2780744224053677 1106 56-57 0.29355435056638157 1106 58-59 0.22104800851845852 1106 60-61 0.30484159972625235 1106 62-63 0.31940355310663193 1106 64-65 0.25030911233588427 1106 66-67 0.12831508558235782 1106 68-69 0.23307420218475983 1106 70-71 0.13559719591658848 1106 72-73 0.2147775503290248 1106 74-75 0.2103058185283757 1106 76-77 0.3504034267057321 1106 78-79 0.26589248562277845 1106 80-81 0.29500369270746774 1106 82-83 0.21804745187500885 1106 84-85 0.3111337493347577 1106 86-87 0.40308320797164754 1106 88-89 0.30578243459397925 1106 90-91 0.3160475775904459 1106 92-93 0.2650855407914392 1106 94-95 0.22407021511098435 1106 96-97 0.3271558111552224 1106 98-99 0.36572443340448757 1106 100 0.3503746288780434 1107 1 0.38758314774865354 1107 2 3.566941888929179 1107 3 2.1447293279595883 1107 4 0.9837090807943056 1107 5 0.28249562717660837 1107 6 0.14817903648075514 1107 7 0.10395809813752521 1107 8 0.1310164793444315 1107 9 0.16423451408680023 1107 10-11 0.13238792735489824 1107 12-13 0.05710844692439565 1107 14-15 0.014066607275402987 1107 16-17 0.04796042711147663 1107 18-19 0.1337785291963698 1107 20-21 0.09256010131669967 1107 22-23 0.14872370749252894 1107 24-25 0.08037536683209368 1107 26-27 -0.023602294577763416 1107 28-29 0.03767955101177023 1107 30-31 0.048352019732519125 1107 32-33 0.13849992188907123 1107 34-35 0.24689917131100003 1107 36-37 0.08459151913226748 1107 38-39 -0.01161821550444131 1107 40-41 0.038310902589003604 1107 42-43 0.1847521434833368 1107 44-45 0.32447875871395837 1107 46-47 0.20411693243890738 1107 48-49 0.022346211753294654 1107 50-51 -0.04565019353785971 1107 52-53 -0.04319346322125028 1107 54-55 0.008029234778483385 1107 56-57 -0.01255699790018383 1107 58-59 0.16093501173089209 1107 60-61 0.050004876021510825 1107 62-63 0.15424760191245213 1107 64-65 -0.015267902231499875 1107 66-67 0.07066517135950079 1107 68-69 -0.002982334497744432 1107 70-71 -0.017082824333122915 1107 72-73 -0.004658560686799262 1107 74-75 0.07455504591039386 1107 76-77 0.14964493743679697 1107 78-79 0.03542748896827419 1107 80-81 0.12720031865642056 1107 82-83 0.03489839819559393 1107 84-85 0.16626748787023615 1107 86-87 0.13200678020930212 1107 88-89 0.049479434594331906 1107 90-91 -0.03682968157433919 1107 92-93 0.06499316274276623 1107 94-95 0.012039334300496307 1107 96-97 0.03963472203673746 1107 98-99 0.13616234035215058 1107 100 -0.020004952731863312 1108 1 0.4432791939933054 1108 2 3.674330168738482 1108 3 2.145846547062469 1108 4 0.8124042438563563 1108 5 0.2109579230524048 1108 6 0.06497716597705505 1108 7 0.05313142134590265 1108 8 0.032295546472496994 1108 9 2.0922713843418705E-4 1108 10-11 0.08768927558402595 1108 12-13 -0.05301664997463007 1108 14-15 -0.07600391664309569 1108 16-17 -0.0898086632861137 1108 18-19 -0.02444717485629866 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0.42121224294327675 2210 58-59 0.5141365443589976 2210 60-61 0.5011686933548631 2210 62-63 0.6070771863422593 2210 64-65 0.2674706602735313 2210 66-67 0.414554459912722 2210 68-69 0.45942220496006314 2210 70-71 0.36455235216880766 2210 72-73 0.48174263252604277 2210 74-75 0.03288014511697668 2210 76-77 0.3729285483610951 2210 78-79 0.5739419171110995 2210 80-81 0.619972187390502 2210 82-83 0.5952941231671787 2210 84-85 0.4956475290760167 2210 86-87 0.5952485318274299 2210 88-89 0.6136153407765761 2210 90-91 0.6554347696761127 2210 92-93 0.7329265179990543 2210 94-95 0.6621492875566943 2210 96-97 0.5171004231560374 2210 98-99 0.5629601073350798 2210 100 0.41392923101427215 2211 1 0.4931257804176248 2211 2 3.9946662291495585 2211 3 2.2216831980725473 2211 4 1.1313287077587404 2211 5 0.615774673564971 2211 6 0.306303535125231 2211 7 0.28806063909119217 2211 8 0.23192959761076537 2211 9 0.0754958422675358 2211 10-11 0.14285148478896303 2211 12-13 0.2155648122013858 2211 14-15 0.2792443172773744 2211 16-17 0.2656866579706829 2211 18-19 0.4066304473445328 2211 20-21 0.3734593311944181 2211 22-23 0.3761157327686604 2211 24-25 0.3444485294534658 2211 26-27 0.30024516163184245 2211 28-29 0.2628841241625679 2211 30-31 0.2915046375716486 2211 32-33 0.44052309867863926 2211 34-35 0.4007283796867611 2211 36-37 0.07646482530615373 2211 38-39 0.2176376658432133 2211 40-41 0.3911845731321222 2211 42-43 0.46442995574676615 2211 44-45 0.4251463876534416 2211 46-47 0.3114453531000194 2211 48-49 0.37079979904338245 2211 50-51 0.2509722180608591 2211 52-53 0.33101401050652157 2211 54-55 0.4140818375141393 2211 56-57 0.40897148079411494 2211 58-59 0.3495044381376644 2211 60-61 0.3384820043158143 2211 62-63 0.5098631238872358 2211 64-65 0.34434373921865813 2211 66-67 0.4214954678960794 2211 68-69 0.38383854470960443 2211 70-71 0.28034337930274944 2211 72-73 0.46318311044623783 2211 74-75 0.3056219134619482 2211 76-77 0.34891695646128795 2211 78-79 0.33286273613413186 2211 80-81 0.27776295762339487 2211 82-83 -0.24840065217191665 2211 84-85 0.2267567613934176 2211 86-87 0.37220003812630154 2211 88-89 0.3317455128698654 2211 90-91 0.3193534958212254 2211 92-93 0.3253030961097281 2211 94-95 0.4489081905938619 2211 96-97 0.35394415267619195 2211 98-99 0.3502337483741158 2211 100 0.2017544606166659 2212 1 0.5564466898473661 2212 2 4.072472788970654 2212 3 2.2202951374438946 2212 4 0.9781090531432568 2212 5 0.5702199462696527 2212 6 0.4977673815657795 2212 7 0.35168034340228616 2212 8 0.45445972557697445 2212 9 0.3769090613009567 2212 10-11 0.31507318934853856 2212 12-13 0.29704139314311107 2212 14-15 0.46521291586105207 2212 16-17 0.336414388123508 2212 18-19 0.4725470208319251 2212 20-21 0.4736849803433856 2212 22-23 0.4607683293645053 2212 24-25 0.39699900156180234 2212 26-27 0.23195919443094226 2212 28-29 0.34871093494651717 2212 30-31 0.4446699376089214 2212 32-33 0.33395892921659254 2212 34-35 0.2430404681449545 2212 36-37 0.2404962267224775 2212 38-39 0.35340701607304936 2212 40-41 0.39389935136544096 2212 42-43 0.23414762253767663 2212 44-45 0.3985887407407773 2212 46-47 0.4363184739846062 2212 48-49 0.22106303050070863 2212 50-51 0.21781033459707544 2212 52-53 0.2840687378018387 2212 54-55 0.15921477328256373 2212 56-57 0.5113801651027288 2212 58-59 0.42034103274568935 2212 60-61 0.14473821626760497 2212 62-63 0.1683154160974425 2212 64-65 0.3705827130042252 2212 66-67 0.26173509393657923 2212 68-69 0.38659028405610485 2212 70-71 0.33457589265844234 2212 72-73 0.45881890371248346 2212 74-75 0.42083839998201 2212 76-77 0.49192493881934496 2212 78-79 0.3759499208775807 2212 80-81 0.18464968268240511 2212 82-83 0.616731662401321 2212 84-85 0.425150040061574 2212 86-87 0.40510179610866004 2212 88-89 0.6395648071921443 2212 90-91 0.46783746898559997 2212 92-93 0.47041553243722234 2212 94-95 0.3807494131059741 2212 96-97 0.30698350539081076 2212 98-99 0.41719268737274007 2212 100 0.5873253389866449 2213 1 -0.5060140431369256 2213 2 -25.912527211029346 2213 3 -14.83017170374284 2213 4 -6.015599498340162 2213 5 -2.05702263662738 2213 6 -1.2059368069158936 2213 7 -1.188939202845532 2213 8 -0.8885856496411719 2213 9 -0.9291555111248755 2213 10-11 -0.9658386780162118 2213 12-13 -1.077714278759153 2213 14-15 -1.2506967700028255 2213 16-17 -1.3749076014052903 2213 18-19 -1.3375096981907646 2213 20-21 -1.3339960842290424 2213 22-23 -1.2785750388728374 2213 24-25 -1.3658522200786862 2213 26-27 -1.4216206485009977 2213 28-29 -1.5337323460307957 2213 30-31 -1.5125159262654222 2213 32-33 -1.3610759747973589 2213 34-35 -1.1485651164972808 2213 36-37 -1.3305050821780426 2213 38-39 -1.2854716924784384 2213 40-41 -1.4270365125089057 2213 42-43 -1.3019387648968817 2213 44-45 -1.2984226030637558 2213 46-47 -1.5527543881445425 2213 48-49 -1.5097288543160445 2213 50-51 -1.416238531022472 2213 52-53 -1.4668693075733756 2213 54-55 -1.4112477049024292 2213 56-57 -1.2818789972009412 2213 58-59 -1.5754857560850226 2213 60-61 -1.5074978221268083 2213 62-63 -1.3849175681957533 2213 64-65 -1.3565682468561562 2213 66-67 -1.4222679601646462 2213 68-69 -1.5248473250189392 2213 70-71 -1.310707702454991 2213 72-73 -1.3403652149611602 2213 74-75 -1.4736074987963477 2213 76-77 -1.3720126702556996 2213 78-79 -1.562136902857148 2213 80-81 -1.4074205616456865 2213 82-83 -1.3141583899546987 2213 84-85 -1.4526815480710589 2213 86-87 -1.1805373836470139 2213 88-89 -1.3928741107834242 2213 90-91 -1.2646363530039366 2213 92-93 -1.3291830714022197 2213 94-95 -1.326632785846904 2213 96-97 -1.500623388151947 2213 98-99 -1.3865464051228926 2213 100 -1.2010167203501751 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4.0 3 6.0 4 15.0 5 24.0 6 33.0 7 40.0 8 31.0 9 77.0 10 269.0 11 1325.0 12 3867.0 13 7048.0 14 9559.0 15 10839.0 16 11880.0 17 12314.0 18 12671.0 19 13524.0 20 14952.0 21 16581.0 22 18301.0 23 19728.0 24 20308.0 25 21376.0 26 23407.0 27 26593.0 28 31296.0 29 37397.0 30 44884.0 31 55385.0 32 70111.0 33 93258.0 34 129625.0 35 183643.0 36 278561.0 37 486044.0 38 551010.0 39 5235.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 37.820164928027964 21.671079906294413 10.535453328794782 29.973301836882836 2 12.37151348061155 23.241743945958206 37.57289316958478 26.813849403845463 3 14.760306636016935 28.20572887106264 29.33533102299589 27.698633469924534 4 9.972009394423315 19.16359240359658 39.1527471180621 31.711651083917996 5 11.106882868777857 39.11697991146967 35.910099715544995 13.86603750420748 6 28.42212244059446 41.21999857914502 18.191695057370413 12.16618392289011 7 24.811549434173273 33.90522232687928 24.370813529171723 16.91241470977572 8 23.253573839441 38.252840883383975 21.45493587086943 17.038649406305595 9 23.93972709339993 17.313598277141356 22.831451218850102 35.91522341060861 10-11 22.276614920208626 28.543445488864677 29.811891249190143 19.36804834173655 12-13 22.916091705447332 26.0300665884267 30.39557181080888 20.658269895317094 14-15 20.507279867181815 27.47197758432966 27.400650472494043 24.620092075994478 16-17 19.775652067304833 31.22777460974584 28.218682119323642 20.777891203625686 18-19 19.621649631671815 30.106851250329946 30.396524196071063 19.87497492192718 20-21 21.065860229645473 29.42027274470849 29.471794607348222 20.042072418297817 22-23 20.157039406636486 29.44666418481951 29.96108958216761 20.435206826376394 24-25 20.3435170786031 29.09389816616594 29.726548753425263 20.8360360018057 26-27 20.20249122553099 29.226874298947063 29.724305279154756 20.84632919636719 28-29 19.92118643722801 29.25474050643504 30.08571155882498 20.738361497511974 30-31 20.636163899972903 29.572372899986927 29.393126247093036 20.398336952947133 32-33 19.751419537530715 29.50252476544928 30.18543588750972 20.560619809510285 34-35 19.96968554690724 28.991073223446644 30.21305818923265 20.82618304041347 36-37 20.436042534668243 29.577310321045164 29.51609748376119 20.470549660525407 38-39 20.12351759439411 29.515418103732888 29.861173297297007 20.499891004576 40-41 20.446506890654295 29.324679019182753 29.556376322914492 20.672437767248457 42-43 20.012039466111638 29.46831193976287 29.87813771801463 20.641510876110864 44-45 20.435910059092382 29.04294130856237 29.711702173127946 20.809446459217302 46-47 20.49192062128295 29.64107154528507 29.412516348390856 20.454491485041125 48-49 20.25898218355292 29.358348253503262 29.620872375324225 20.761797187619592 50-51 20.68114414230829 29.32370721504269 29.170386229111173 20.824762413537844 52-53 20.15188622827628 29.612883730314188 29.43062568476822 20.804604356641313 54-55 20.571405178423042 29.39473635404241 29.13289301493256 20.90096545260199 56-57 20.630920519673666 29.321993496616006 29.135549284460403 20.91153669924993 58-59 20.18562182792202 28.989598320678567 29.72122576714477 21.103554084254643 60-61 20.759626496242575 29.389879034173017 28.91133017180359 20.939164297780824 62-63 20.1517354178672 29.619548747027125 29.39390869097557 20.834807144130103 64-65 20.201456395216034 28.914361808231497 29.53284804919122 21.351333747361252 66-67 20.363365541473776 29.314134649070134 29.206796797414846 21.115703012041244 68-69 20.501252146472186 29.238547473903058 29.289234978313623 20.970965401311133 70-71 20.700528317386706 29.183914977044722 28.969290279620534 21.14626642594803 72-73 20.26398140667648 29.40202724403194 29.47098773864764 20.863003610643936 74-75 20.60509463382065 28.867601311633212 29.201435396093235 21.3258686584529 76-77 20.521846625372824 29.3777156188965 29.100149175284123 21.00028858044655 78-79 20.51743809343964 29.27763335395095 29.26250371739015 20.94242483521926 80-81 20.734937399448103 29.030138091647693 29.04463429979244 21.190290209111758 82-83 20.147834973167683 29.389047222661308 29.407500023179068 21.05561778099194 84-85 20.381395708563314 28.853051660922997 29.246338000108096 21.519214630405596 86-87 20.5088201845605 29.297413226783004 29.027245818872732 21.166520769783766 88-89 20.20578295325674 28.977790139830645 29.37951916890823 21.43690773800438 90-91 20.546473468929946 28.97837153809833 29.064619450871703 21.410535542100018 92-93 20.088736951312942 29.326497348429005 29.307660928153865 21.277104772104188 94-95 20.10803215548526 28.725233680363115 29.443013467613753 21.723720696537864 96-97 20.268849675865827 29.06245187847224 29.25083388035945 21.417864565302484 98-99 20.170128726017634 28.896467451275505 29.33783253316739 21.59557128953947 100 20.411330583402716 28.74759539529234 29.048530344538147 21.792543676766797 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 16.0 1 12.0 2 15.0 3 18.0 4 16.0 5 24.0 6 43.0 7 76.0 8 124.0 9 149.0 10 141.5 11 160.0 12 197.0 13 254.0 14 343.0 15 449.0 16 561.5 17 707.0 18 900.5 19 1232.5 20 1813.5 21 2635.5 22 3824.0 23 5566.5 24 8173.5 25 11754.5 26 16439.0 27 22613.5 28 30062.0 29 38265.0 30 47003.5 31 55933.5 32 64418.0 33 71842.0 34 78644.0 35 85002.0 36 90319.5 37 94593.0 38 97112.5 39 98421.5 40 98504.5 41 96170.0 42 92758.0 43 88728.5 44 83866.0 45 78519.5 46 73389.0 47 68928.0 48 64450.5 49 60823.0 50 61012.0 51 57050.0 52 48066.5 53 42432.0 54 38607.5 55 35595.5 56 32509.5 57 29003.5 58 25461.0 59 21870.0 60 18508.5 61 15193.0 62 12110.0 63 9525.5 64 7318.5 65 5623.0 66 4295.5 67 3170.5 68 2303.0 69 1703.0 70 1188.0 71 830.5 72 623.5 73 438.5 74 301.0 75 196.5 76 116.5 77 61.0 78 39.0 79 22.5 80 8.5 81 6.5 82 6.5 83 4.5 84 4.0 85 3.0 86 2.5 87 3.0 88 1.5 89 0.5 90 0.0 91 0.0 92 0.5 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content warn #Base N-Count 1 0.7546961610802358 2 5.270662679126148 3 0.0 4 0.005517313737523295 5 0.17375015884888936 6 0.058067465893277964 7 0.03740015131911283 8 0.044907315912792074 9 0.042555673991880504 10-11 0.04327925612139175 12-13 0.04192253962855816 14-15 0.04438724125720586 16-17 0.034845001924276225 18-19 0.028242314992486053 20-21 0.023018956495076702 22-23 0.02270238931341553 24-25 0.020034180210842786 26-27 0.011803433487652297 28-29 0.019627165262992706 30-31 0.026229852194782882 32-33 0.0011532090189085578 34-35 0.0029169404595922343 36-37 0.003776194238386846 38-39 0.005381642088239936 40-41 0.0066252988733374 42-43 0.007213209353565293 44-45 0.008660373612587797 46-47 0.01702679198506165 48-49 0.02564194171455499 50-51 0.022589329605679394 52-53 0.027993583635466557 54-55 0.02478268793576038 56-57 0.016823284511136606 58-59 0.014833433654980665 60-61 0.012029552903124564 62-63 0.018044329354686844 64-65 0.01899403089967036 66-67 0.02010201603548447 68-69 0.027903135869277656 70-71 0.027948359752372105 72-73 0.024194777455532487 74-75 0.024511344637193656 76-77 0.018044329354686844 78-79 0.014856045596527891 80-81 0.013476717162147066 82-83 0.00789156759998209 84-85 0.010695448351838192 86-87 0.008343806430926625 88-89 0.006941866054998573 90-91 0.006919254113451347 92-93 0.003572686764461806 94-95 0.0012436567850974641 96-97 0.001017537369625198 98-99 5.879104802278921E-4 100 0.11025582698427701 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 2211221.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 91.0856785730649 #Duplication Level Percentage of deduplicated Percentage of total 1 93.37813396796946 85.0541069635906 2 5.605857089857859 10.212265940266601 3 0.6714113544500114 1.8346787646521967 4 0.16256634981645635 0.5922986514471268 5 0.05691653322351713 0.25921405253452257 6 0.035373115354837344 0.1933190529203197 7 0.021792290691292536 0.1389475909694506 8 0.01252167193050969 0.09124359877278196 9 0.01051369101053792 0.08618820120021409 >10 0.040737861090100046 0.6170529228533272 >50 0.001934473535889813 0.12051728851224812 >100 0.0017438637601519774 0.324584563695765 >500 2.986423855922235E-4 0.17182624676246872 >1k 1.9909492372790453E-4 0.30375616182249715 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 2439 0.11030105086737146 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 4.5223883094453246E-5 0.0 5 0.0 0.0 0.0 4.5223883094453246E-5 0.0 6 0.0 0.0 0.0 4.5223883094453246E-5 0.0 7 0.0 0.0 0.0 4.5223883094453246E-5 0.0 8 4.5223883094453246E-5 0.0 0.0 4.5223883094453246E-5 0.0 9 4.5223883094453246E-5 0.0 0.0 9.044776618890649E-5 0.0 10-11 4.5223883094453246E-5 2.2611941547226623E-5 0.0 9.044776618890649E-5 0.0 12-13 4.5223883094453246E-5 1.8089553237781299E-4 0.0 9.044776618890649E-5 0.0 14-15 4.5223883094453246E-5 1.8089553237781299E-4 0.0 9.044776618890649E-5 0.0 16-17 4.5223883094453246E-5 1.8089553237781299E-4 0.0 9.044776618890649E-5 4.5223883094453246E-5 18-19 4.5223883094453246E-5 1.8089553237781299E-4 0.0 9.044776618890649E-5 9.044776618890649E-5 20-21 4.5223883094453246E-5 1.8089553237781299E-4 0.0 9.044776618890649E-5 9.044776618890649E-5 22-23 4.5223883094453246E-5 1.8089553237781299E-4 0.0 1.8089553237781299E-4 9.044776618890649E-5 24-25 4.5223883094453246E-5 1.8089553237781299E-4 0.0 2.2611941547226623E-4 9.044776618890649E-5 26-27 4.5223883094453246E-5 1.8089553237781299E-4 0.0 3.165671816611727E-4 9.044776618890649E-5 28-29 4.5223883094453246E-5 1.8089553237781299E-4 0.0 4.5223883094453246E-4 9.044776618890649E-5 30-31 4.5223883094453246E-5 1.8089553237781299E-4 0.0 0.0016958956160419967 9.044776618890649E-5 32-33 4.5223883094453246E-5 1.8089553237781299E-4 0.0 0.005946940626920602 9.044776618890649E-5 34-35 6.783582464167987E-5 1.8089553237781299E-4 0.0 0.012549627558710776 9.044776618890649E-5 36-37 9.044776618890649E-5 1.8089553237781299E-4 0.0 0.023132016202812834 9.044776618890649E-5 38-39 9.044776618890649E-5 2.2611941547226623E-4 0.0 0.0417868679792748 9.044776618890649E-5 40-41 9.044776618890649E-5 2.2611941547226623E-4 0.0 0.06331343633223455 9.044776618890649E-5 42-43 9.044776618890649E-5 2.2611941547226623E-4 0.0 0.08563142263934723 9.044776618890649E-5 44-45 9.044776618890649E-5 2.2611941547226623E-4 0.0 0.11258485696364134 9.044776618890649E-5 46-47 9.044776618890649E-5 2.2611941547226623E-4 0.0 0.147972545485051 9.044776618890649E-5 48-49 9.044776618890649E-5 2.2611941547226623E-4 0.0 0.1840385922528775 9.044776618890649E-5 50-51 9.044776618890649E-5 2.2611941547226623E-4 0.0 0.2230441914218434 9.044776618890649E-5 52-53 9.044776618890649E-5 2.2611941547226623E-4 0.0 0.266006880361574 9.044776618890649E-5 54-55 9.044776618890649E-5 2.2611941547226623E-4 0.0 0.3135145695522971 9.044776618890649E-5 56-57 9.044776618890649E-5 2.2611941547226623E-4 0.0 0.3718533787441418 9.044776618890649E-5 58-59 9.044776618890649E-5 2.2611941547226623E-4 0.0 0.4387168898992909 9.044776618890649E-5 60-61 9.044776618890649E-5 2.2611941547226623E-4 0.0 0.5084747295724851 9.044776618890649E-5 62-63 9.044776618890649E-5 2.2611941547226623E-4 0.0 0.5823253306657272 9.044776618890649E-5 64-65 9.044776618890649E-5 2.2611941547226623E-4 0.0 0.6682280966036411 9.044776618890649E-5 66-67 9.044776618890649E-5 2.2611941547226623E-4 0.0 0.764894146718035 9.044776618890649E-5 68-69 9.044776618890649E-5 2.9395524011394613E-4 0.0 0.8779086305710736 9.044776618890649E-5 70-71 1.1305970773613312E-4 3.165671816611727E-4 0.0 1.0059374436114707 9.044776618890649E-5 72-73 1.3567164928335974E-4 3.165671816611727E-4 0.0 1.1369284209945545 9.044776618890649E-5 74-75 1.3567164928335974E-4 3.165671816611727E-4 0.0 1.271062458252703 9.044776618890649E-5 76-77 1.3567164928335974E-4 3.165671816611727E-4 0.0 1.4245523174752772 9.044776618890649E-5 78-79 1.3567164928335974E-4 3.165671816611727E-4 0.0 1.600835013777456 9.044776618890649E-5 80-81 1.3567164928335974E-4 3.165671816611727E-4 0.0 1.7995261441529364 9.044776618890649E-5 82-83 1.3567164928335974E-4 3.165671816611727E-4 0.0 2.0113548125673555 9.044776618890649E-5 84-85 1.3567164928335974E-4 3.165671816611727E-4 0.0 2.2293791529657145 9.044776618890649E-5 86-87 1.3567164928335974E-4 3.165671816611727E-4 0.0 2.461716852363468 9.044776618890649E-5 88 1.3567164928335974E-4 3.165671816611727E-4 0.0 2.658305072175056 9.044776618890649E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 5695 0.0 23.997375 1 CAACGCA 6120 0.0 21.904915 5 AACGCAG 6220 0.0 21.592152 6 ATCAACG 6275 0.0 21.587252 3 TCAACGC 6190 0.0 21.581211 4 TATCAAC 6555 0.0 21.133007 2 ACGCAGA 6835 0.0 19.370571 7 CGCAGAG 6905 0.0 19.1742 8 TATACCG 125 0.006383205 18.81517 5 TACCGGT 155 0.0010412084 18.174036 7 ACCGCTC 165 0.001583252 17.072578 8 GAGTACT 2770 0.0 17.034058 12-13 GCAGAGT 7880 0.0 16.325111 9 GTACATG 7130 0.0 15.821992 1 ACATGGG 7125 0.0 15.38118 3 TACATGG 7350 0.0 15.266901 2 GGTATCA 4400 0.0 14.795969 1 TCTATAC 995 0.0 14.654034 3 GCACCGT 195 0.004815842 14.448972 6 TAGGGTG 690 0.0 14.315889 5 >>END_MODULE