FastQCFastQC Report
Wed 25 May 2016
SRR1295090_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295090_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2153919
Sequences flagged as poor quality0
Sequence length100
%GC48

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT448212.0809046208330026No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA351691.6327912052403086No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC154470.7171578875528746No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG150980.7009548641337023No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC138160.6414354485939351No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG104730.4862299835787697No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG102780.4771767183445617No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC93960.43622810328522105No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG87440.4059576985021257No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT79740.37020890757730446No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT76630.35577011020377275No Hit
GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC55730.2587376776935437No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC52190.24230251926836616No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA49290.2288386889200569No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT42620.1978718791189455No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC42450.19708262009852737No Hit
TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC40860.1897007269075578No Hit
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG40570.18835434387272687No Hit
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA40470.1878900738607162No Hit
CNCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT40030.18584728580786927No Hit
CCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA38990.18101887768295835No Hit
CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTC38100.17688687457606345No Hit
GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT36690.17034066740671305No Hit
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG35960.16695149631903522No Hit
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG34520.16026600814608163No Hit
CTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT32400.15042348389145554No Hit
GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA31100.1443879737353169No Hit
ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC27990.12994917636178518No Hit
GNACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA27450.1274421182969276No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG25850.12001379810475696No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGAAAGCAGTGGTA24880.1155103789882535No Hit
CCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGA24800.11513896297864498No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTTCCATGTACTCTGCGTTGAT23620.10966057683691913No Hit
ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA23110.10729279977566472No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACAAA8950.027.0245191
TACGACT5200.026.0823148
GATACGA4450.025.9463986
CTGTGCG7700.025.4523499
TGATACG5050.023.7362545
GAACAAA14450.023.6053941
ACGACTG6300.023.1319089
TACTAAG1652.4331236E-422.5473062
ATACGAC5400.022.1587647
TATACGA1603.389748E-521.8509545
AGTACTC15900.021.0460225
ATCGTTC1200.00344635620.930258
ACTGTGC11550.020.8758878
ACGGTAC1350.00360914220.764073
ACTAAGT1905.2311087E-520.6547873
TAGACTG3252.4920155E-1019.9780165
GTAGACC1757.143439E-419.2215983
CGGTACT2059.380111E-519.1440534
TGTACTA7600.019.0579745
TACTGTG11150.018.7802987
Produced by FastQC (version 0.11.5)