##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1295090_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2153919 Sequences flagged as poor quality 0 Sequence length 100 %GC 48 >>END_MODULE >>Per base sequence quality warn #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 26.387658960248736 33.0 27.0 33.0 6.0 33.0 2 22.454808189165888 27.0 6.0 33.0 2.0 33.0 3 24.982616337940286 33.0 15.0 33.0 15.0 33.0 4 25.01490028176547 33.0 6.0 37.0 2.0 37.0 5 27.209445201978347 33.0 15.0 37.0 2.0 37.0 6 27.939774894041978 33.0 15.0 37.0 6.0 37.0 7 27.825177734167347 33.0 15.0 37.0 6.0 37.0 8 28.50529755297205 33.0 22.0 37.0 6.0 37.0 9 29.448265696156632 33.0 27.0 37.0 15.0 37.0 10-11 28.99574914377003 35.0 24.5 37.0 10.5 37.0 12-13 27.789733504370407 33.0 15.0 37.0 4.0 37.0 14-15 29.581175754520018 33.0 27.0 37.0 10.5 40.0 16-17 29.927708748564825 35.0 27.0 38.5 6.0 40.0 18-19 30.053121542639254 35.0 27.0 40.0 10.5 40.0 20-21 29.638130078243428 33.0 27.0 38.5 6.0 40.0 22-23 30.504102986231146 37.0 27.0 40.0 15.0 40.0 24-25 30.652053071633613 37.0 27.0 40.0 15.0 40.0 26-27 29.062383729378865 33.0 22.0 38.5 4.0 40.0 28-29 29.721792230812767 33.0 27.0 38.5 6.0 40.0 30-31 30.328021387990912 33.0 27.0 40.0 15.0 40.0 32-33 28.951645349709064 33.0 22.0 37.0 6.0 40.0 34-35 28.625152106462686 33.0 22.0 37.0 6.0 40.0 36-37 29.02659524336802 33.0 22.0 37.0 6.0 40.0 38-39 29.03210055717044 33.0 24.5 37.0 6.0 40.0 40-41 27.98498829343165 33.0 22.0 37.0 6.0 40.0 42-43 28.48934244973929 33.0 22.0 37.0 6.0 40.0 44-45 28.272987285037182 33.0 22.0 37.0 6.0 40.0 46-47 27.80767730819961 33.0 22.0 37.0 6.0 40.0 48-49 27.626752909464095 33.0 18.5 37.0 6.0 40.0 50-51 27.762548638087132 33.0 22.0 37.0 6.0 40.0 52-53 28.096394525513727 33.0 22.0 37.0 6.0 40.0 54-55 27.940511458416033 33.0 22.0 37.0 6.0 40.0 56-57 27.691962650406076 33.0 22.0 37.0 6.0 40.0 58-59 27.48882293159585 33.0 22.0 37.0 6.0 40.0 60-61 27.32523298229878 33.0 22.0 37.0 6.0 40.0 62-63 26.960862037987503 33.0 22.0 37.0 6.0 37.0 64-65 26.700692319441913 33.0 22.0 37.0 6.0 37.0 66-67 26.886367361075322 30.0 22.0 37.0 6.0 37.0 68-69 26.35215344681021 30.0 18.5 37.0 6.0 37.0 70-71 26.147554527352234 27.0 18.5 33.0 6.0 37.0 72-73 25.910911691665284 27.0 22.0 33.0 6.0 37.0 74-75 25.91789291983589 27.0 22.0 33.0 6.0 37.0 76-77 25.51163483863599 27.0 22.0 33.0 6.0 37.0 78-79 25.113569962473058 27.0 15.0 33.0 6.0 37.0 80-81 25.08906300561906 27.0 15.0 33.0 6.0 37.0 82-83 25.369021537021588 27.0 22.0 33.0 6.0 37.0 84-85 25.158053297268836 27.0 18.5 33.0 6.0 37.0 86-87 24.625500773241704 27.0 15.0 33.0 6.0 37.0 88-89 24.520542555221436 27.0 15.0 33.0 6.0 37.0 90-91 24.73673058271922 27.0 15.0 33.0 6.0 37.0 92-93 24.45446277227695 27.0 15.0 33.0 6.0 37.0 94-95 24.48986127147771 27.0 15.0 33.0 6.0 37.0 96-97 24.73539348508463 27.0 15.0 33.0 6.0 37.0 98-99 24.755987806412406 27.0 15.0 33.0 6.0 37.0 100 24.079233713059775 27.0 15.0 33.0 6.0 37.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 7.371304588539388 1101 2 11.318207158951385 1101 3 8.357038181885137 1101 4 12.844558680033654 1101 5 10.985904008923985 1101 6 10.043093558110431 1101 7 9.462931177861101 1101 8 9.01774223175163 1101 9 7.299225984891276 1101 10-11 7.834793381903513 1101 12-13 9.207292243612603 1101 14-15 7.985100109747119 1101 16-17 8.172673937091702 1101 18-19 8.244028537980839 1101 20-21 7.33047402851566 1101 22-23 7.5206289341097765 1101 24-25 7.489757127858585 1101 26-27 8.731599229529422 1101 28-29 8.3862448996564 1101 30-31 5.4169717165453015 1101 32-33 7.0394784753845485 1101 34-35 8.807226177569614 1101 36-37 8.226735242559588 1101 38-39 8.18120245783754 1101 40-41 9.112470749821195 1101 42-43 8.62742386712149 1101 44-45 8.763456909266143 1101 46-47 7.646849775107228 1101 48-49 5.76461125203846 1101 50-51 6.854104549272169 1101 52-53 6.040443276434949 1101 54-55 4.174655765225907 1101 56-57 6.301614189890053 1101 58-59 6.316114093649009 1101 60-61 6.814278055210927 1101 62-63 6.2037450048523795 1101 64-65 6.7901059929547465 1101 66-67 5.939749281438893 1101 68-69 6.783367780642873 1101 70-71 6.316318415757408 1101 72-73 5.5383065759020305 1101 74-75 4.9581781118393415 1101 76-77 5.069177352691696 1101 78-79 5.132862441071467 1101 80-81 5.055674222571625 1101 82-83 4.653100533528875 1101 84-85 4.347501237633679 1101 86-87 4.1961968076531875 1101 88-89 4.215288822915198 1101 90-91 3.93206344877402 1101 92-93 3.8398298361206784 1101 94-95 3.85226179207422 1101 96-97 3.918129262109069 1101 98-99 3.7018947045391 1101 100 3.6932867907007427 1102 1 6.921446131682259 1102 2 10.535358179150041 1102 3 8.281070821301924 1102 4 12.724568582541213 1102 5 10.916977522797797 1102 6 9.645132762569833 1102 7 7.043956561825002 1102 8 8.091697507263287 1102 9 6.539667146211958 1102 10-11 7.639414029472196 1102 12-13 9.309712619607875 1102 14-15 6.038358213663624 1102 16-17 7.440659599149967 1102 18-19 7.5867083907607835 1102 20-21 7.139815943720983 1102 22-23 6.856596851358862 1102 24-25 7.2130145061213575 1102 26-27 8.702707095832743 1102 28-29 7.943903691308289 1102 30-31 4.083136570613899 1102 32-33 4.69791127659591 1102 34-35 5.2575103121222675 1102 36-37 5.3009476145671 1102 38-39 4.773792349435897 1102 40-41 6.435980850776527 1102 42-43 7.112132431751302 1102 44-45 6.458273473401459 1102 46-47 5.31200664373155 1102 48-49 3.4239395533291663 1102 50-51 5.169558402755545 1102 52-53 6.061094541168977 1102 54-55 5.470060316728524 1102 56-57 6.421084943424066 1102 58-59 6.165797860440708 1102 60-61 6.052637638593513 1102 62-63 4.4221720866833465 1102 64-65 6.2919486835161145 1102 66-67 3.8280928832695302 1102 68-69 6.065275556831381 1102 70-71 5.281759495747039 1102 72-73 5.008579262657104 1102 74-75 4.411472647549278 1102 76-77 3.3508977059865614 1102 78-79 4.389488481973395 1102 80-81 4.52018514641648 1102 82-83 4.010008192239194 1102 84-85 3.107084746732454 1102 86-87 3.4271253308511476 1102 88-89 3.5083905308311643 1102 90-91 3.4092016511986962 1102 92-93 3.187576432777572 1102 94-95 3.1796509098347165 1102 96-97 3.5782175343157867 1102 98-99 3.2412430747190157 1102 100 3.2728122944923967 1103 1 6.8174481565205625 1103 2 10.989713958583131 1103 3 8.121537549772622 1103 4 12.22697888566189 1103 5 10.624142846415364 1103 6 9.378245243742288 1103 7 8.444867793086601 1103 8 8.40432243892229 1103 9 7.221319129019204 1103 10-11 7.608996479504935 1103 12-13 8.977681816998274 1103 14-15 8.811480159928696 1103 16-17 8.280391189220694 1103 18-19 7.559471668362441 1103 20-21 8.619119562498177 1103 22-23 7.155929864867151 1103 24-25 5.460052477782266 1103 26-27 6.197840028520375 1103 28-29 7.377358741842809 1103 30-31 6.310824991866706 1103 32-33 8.143357875537468 1103 34-35 7.504059767231151 1103 36-37 4.333061128688026 1103 38-39 6.800732747445206 1103 40-41 7.037554071263482 1103 42-43 7.789923688642123 1103 44-45 5.775148512569221 1103 46-47 6.528275771106195 1103 48-49 5.772791154507331 1103 50-51 6.7006617937654305 1103 52-53 6.444827061541613 1103 54-55 5.145014071755504 1103 56-57 6.076620671987687 1103 58-59 5.702311400061195 1103 60-61 5.258968823912678 1103 62-63 3.1552082582478747 1103 64-65 5.266336747991119 1103 66-67 4.454576801256014 1103 68-69 5.218227428564251 1103 70-71 3.7486381545563816 1103 72-73 4.539468851703528 1103 74-75 3.494320705800657 1103 76-77 3.629404679469175 1103 78-79 2.9179161745005118 1103 80-81 4.201576190882967 1103 82-83 3.5500499369712664 1103 84-85 3.467551345544347 1103 86-87 3.0801406813729173 1103 88-89 3.294950801645186 1103 90-91 3.0073420198186724 1103 92-93 2.5766892532239005 1103 94-95 3.0817017879489548 1103 96-97 3.2464548282933556 1103 98-99 2.999994233544566 1103 100 3.0636704844987257 1104 1 6.3845788092790805 1104 2 9.733153610440379 1104 3 7.336591239461175 1104 4 12.13308002612479 1104 5 9.693434210201584 1104 6 8.23950338819117 1104 7 4.040026095703205 1104 8 6.743261924106697 1104 9 6.678466101540817 1104 10-11 7.278473675505342 1104 12-13 7.523333873613087 1104 14-15 7.328005646240715 1104 16-17 7.125230367077762 1104 18-19 7.035419863060099 1104 20-21 7.48172853429822 1104 22-23 6.48972935629779 1104 24-25 6.041028545025721 1104 26-27 8.354435128750417 1104 28-29 7.334320042192754 1104 30-31 6.40935321810645 1104 32-33 7.799513087504614 1104 34-35 7.944500085322485 1104 36-37 6.717352613649293 1104 38-39 7.325065655979994 1104 40-41 7.043094651413 1104 42-43 7.223619884065915 1104 44-45 6.749605819858605 1104 46-47 6.650079413582208 1104 48-49 5.512862231671139 1104 50-51 5.660886955282617 1104 52-53 5.903150415081459 1104 54-55 4.833866905353823 1104 56-57 5.93635239959168 1104 58-59 3.8558155078950946 1104 60-61 4.090261020834465 1104 62-63 3.6933155681208056 1104 64-65 2.7938447398280353 1104 66-67 3.210310324079675 1104 68-69 3.98351011621752 1104 70-71 2.4039535777613565 1104 72-73 2.289422142154617 1104 74-75 3.0924076491213732 1104 76-77 3.6392835318638 1104 78-79 4.18937670870411 1104 80-81 2.9111789470893044 1104 82-83 2.801662159463273 1104 84-85 2.85638630010828 1104 86-87 0.5276496883365951 1104 88-89 0.35351399031380737 1104 90-91 2.096148532542742 1104 92-93 2.3407472041585287 1104 94-95 2.47349592847268 1104 96-97 2.9272805698960376 1104 98-99 2.5229061246571334 1104 100 0.5781970398946967 1105 1 6.123050074847615 1105 2 -2.587438585398992 1105 3 0.5345493487922042 1105 4 8.805964514425774 1105 5 9.05528083570341 1105 6 8.443889311196475 1105 7 5.6118926331135235 1105 8 6.26483821762071 1105 9 5.302904923339181 1105 10-11 5.400642320295496 1105 12-13 5.362766172781338 1105 14-15 6.249280255254359 1105 16-17 6.472118145609613 1105 18-19 6.537972592129911 1105 20-21 7.398830511568342 1105 22-23 2.0977490711673035 1105 24-25 4.208584111959663 1105 26-27 7.20491253681471 1105 28-29 6.3146157789505075 1105 30-31 5.6372915389640745 1105 32-33 7.357849813398033 1105 34-35 6.367032840766264 1105 36-37 6.592518228108016 1105 38-39 4.32911201819935 1105 40-41 5.645522162442724 1105 42-43 5.774597044231399 1105 44-45 6.346048809191302 1105 46-47 5.562694296790898 1105 48-49 5.153614234805477 1105 50-51 6.029376720113628 1105 52-53 5.816269302044404 1105 54-55 5.41454885678943 1105 56-57 5.290574896825248 1105 58-59 2.1267482588586297 1105 60-61 4.499484296394844 1105 62-63 -0.3232502436969149 1105 64-65 -0.1614734724021396 1105 66-67 0.9686399638846588 1105 68-69 2.920148713592603 1105 70-71 2.741949481087911 1105 72-73 2.4826975813835226 1105 74-75 2.534200072904209 1105 76-77 1.748665650096786 1105 78-79 3.0714636943255194 1105 80-81 1.413911319930019 1105 82-83 0.9849061661753993 1105 84-85 1.2638215726906736 1105 86-87 -0.3212245493660113 1105 88-89 1.6015666989253958 1105 90-91 1.4211143993119322 1105 92-93 1.5833770207271272 1105 94-95 1.0250628112040907 1105 96-97 2.099307024321405 1105 98-99 1.9937526539253057 1105 100 2.032797408508447 1106 1 6.276394290230698 1106 2 10.272888509999305 1106 3 6.796534334280288 1106 4 11.731725042922104 1106 5 9.83304387265559 1106 6 9.297165019473024 1106 7 6.512037888102135 1106 8 7.50179633003032 1106 9 6.875307064129661 1106 10-11 5.760249162855931 1106 12-13 8.083112194795984 1106 14-15 7.881681286709668 1106 16-17 7.608337984144754 1106 18-19 7.200844366568113 1106 20-21 8.097252221936778 1106 22-23 6.159805801209462 1106 24-25 5.0025419028884635 1106 26-27 7.6408118227650945 1106 28-29 6.159452706680721 1106 30-31 5.304789376892476 1106 32-33 7.191931329157384 1106 34-35 6.756340819376472 1106 36-37 5.861864920251659 1106 38-39 7.000670702029769 1106 40-41 6.39099750124274 1106 42-43 5.9555535175416985 1106 44-45 4.243408297176526 1106 46-47 5.737204559273181 1106 48-49 5.612573135069454 1106 50-51 6.30976027840806 1106 52-53 5.373936460742723 1106 54-55 4.961522137659511 1106 56-57 4.545927439013944 1106 58-59 4.6004725913331725 1106 60-61 4.402663379314252 1106 62-63 4.4944523005293355 1106 64-65 4.731323886021787 1106 66-67 2.385143950699419 1106 68-69 2.7571968406273264 1106 70-71 2.4639870581187893 1106 72-73 2.203184511604075 1106 74-75 2.3592752360217553 1106 76-77 3.021599275630514 1106 78-79 3.5265416194612413 1106 80-81 3.243709285092365 1106 82-83 1.5924496906011498 1106 84-85 2.3275189188892753 1106 86-87 2.94555973701452 1106 88-89 2.9235840362493164 1106 90-91 2.899285173537322 1106 92-93 2.531764070031418 1106 94-95 1.0766394255131537 1106 96-97 1.1832856662234832 1106 98-99 1.6086839430633333 1106 100 2.5802353423377333 1107 1 6.165088629339216 1107 2 10.386622700148944 1107 3 6.24818605207588 1107 4 11.529754743868995 1107 5 9.507189516289646 1107 6 8.182514368913292 1107 7 3.822019041171007 1107 8 6.541712540661809 1107 9 5.849530139131364 1107 10-11 4.709472578464823 1107 12-13 6.314576578637574 1107 14-15 6.216518703544747 1107 16-17 7.103011341850419 1107 18-19 6.542996549860192 1107 20-21 5.661765721335033 1107 22-23 5.926173225537898 1107 24-25 4.630692405851757 1107 26-27 7.304128542036651 1107 28-29 6.463651030893349 1107 30-31 5.1028501866292935 1107 32-33 5.0974306592965775 1107 34-35 6.721042447478833 1107 36-37 6.633497450508937 1107 38-39 6.486196031851062 1107 40-41 7.562645472359254 1107 42-43 6.1948881981656925 1107 44-45 4.092205840263333 1107 46-47 3.400202243144296 1107 48-49 4.129175464822627 1107 50-51 4.74352012763261 1107 52-53 3.79236342320781 1107 54-55 5.175841945102913 1107 56-57 5.138451678892235 1107 58-59 2.9199049126847 1107 60-61 2.5824760453602558 1107 62-63 0.27902779043600745 1107 64-65 1.93072157897576 1107 66-67 3.9077088363689363 1107 68-69 4.641116321087829 1107 70-71 3.834143169747115 1107 72-73 1.8787402690009323 1107 74-75 2.136986469246853 1107 76-77 2.827954324564409 1107 78-79 3.6631120456743744 1107 80-81 2.0129068145548885 1107 82-83 1.5559063994274176 1107 84-85 1.9883070839248091 1107 86-87 2.0669491872745205 1107 88-89 2.378249559989637 1107 90-91 1.260466172197603 1107 92-93 1.9963187828995572 1107 94-95 1.9237306401183893 1107 96-97 2.061463985440543 1107 98-99 1.5048564265438813 1107 100 -0.06697122456887072 1108 1 4.2202753910750666 1108 2 -3.0647648199553537 1108 3 0.07665660004315811 1108 4 6.6603293489213655 1108 5 7.191278032859035 1108 6 6.775915573786715 1108 7 4.056819918383503 1108 8 4.994338299495556 1108 9 4.846475783642411 1108 10-11 3.2338955061955694 1108 12-13 2.5772236186355393 1108 14-15 3.8714313704223464 1108 16-17 4.620289077839601 1108 18-19 3.862407304928425 1108 20-21 4.7194911062503735 1108 22-23 4.052555513871344 1108 24-25 2.859648521167699 1108 26-27 4.68368325818868 1108 28-29 6.061468327014307 1108 30-31 4.415943073372919 1108 32-33 4.415708038027304 1108 34-35 2.7454340526769236 1108 36-37 0.9883685809845311 1108 38-39 2.066346693233154 1108 40-41 5.040184492744775 1108 42-43 2.82287145377526 1108 44-45 3.330474326118484 1108 46-47 4.368435407169507 1108 48-49 4.456319571624295 1108 50-51 1.774891005939125 1108 52-53 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-16.68239978633191 2213 48-49 -6.40845458033974 2213 50-51 -23.167899296060025 2213 52-53 -17.839318763368375 2213 54-55 -17.552237083980604 2213 56-57 -17.198484633167293 2213 58-59 -17.62405382324563 2213 60-61 -18.73861381041691 2213 62-63 -16.961605054805258 2213 64-65 -17.02533499942198 2213 66-67 -8.720857629304621 2213 68-69 -21.95365422320246 2213 70-71 -16.485737747944626 2213 72-73 -8.911868293794072 2213 74-75 -4.2579827441866165 2213 76-77 -3.048721477069158 2213 78-79 -4.27948526436688 2213 80-81 -3.2642956331156974 2213 82-83 -3.260417363344704 2213 84-85 -2.9692040576957766 2213 86-87 -3.1815888902827183 2213 88-89 -2.725713931128947 2213 90-91 -2.5818860490056537 2213 92-93 -3.494978686303451 2213 94-95 -3.55982481056083 2213 96-97 -3.2736611838662064 2213 98-99 -3.8127171522477425 2213 100 -3.1646924883048406 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 574.0 3 686.0 4 4328.0 5 13436.0 6 18885.0 7 19177.0 8 17853.0 9 18446.0 10 21015.0 11 25271.0 12 31604.0 13 39605.0 14 37124.0 15 41642.0 16 43882.0 17 44930.0 18 44888.0 19 46155.0 20 46586.0 21 46952.0 22 50678.0 23 54450.0 24 60280.0 25 65404.0 26 71572.0 27 76545.0 28 82126.0 29 86744.0 30 93902.0 31 104635.0 32 119896.0 33 137602.0 34 153806.0 35 161026.0 36 147934.0 37 101099.0 38 23167.0 39 14.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.77535262082226 17.44132728130253 11.548054790765546 25.235265307109657 2 11.920276321594917 23.37663216749135 34.9025237790781 29.800567731835624 3 22.381698282596737 30.660917298178695 25.347376883548623 21.610007535675944 4 13.940664864550456 20.891894456808906 29.506418125656275 35.66102255298436 5 11.35927477743428 40.79313560745206 34.64399518799661 13.203594427117055 6 32.88107038249018 28.793075180770693 20.140186267440406 18.18566816929871 7 24.366099409938982 32.6792190871912 23.053125613074908 19.90155588979491 8 29.38714266762428 32.40499727812113 22.207736032135962 16.000124022118634 9 29.507869247029493 17.307996624116466 18.296438911746435 34.8876952171076 10-11 22.753857338438348 29.899014704098665 24.057722534637033 23.289405422825958 12-13 28.322235696032553 25.879320765318152 23.622310052565044 22.176133486084247 14-15 29.021877635905007 24.924591829170314 22.055149393829502 23.998381141095173 16-17 22.850498290282992 28.413249985312145 30.036453622555104 18.699798101849762 18-19 26.12086050953119 27.851223752110826 28.27628454519812 17.751631193159863 20-21 21.939634550214386 28.776241808469283 27.723458004414503 21.560665636901827 22-23 19.69635033332808 30.169540275172967 21.96470997305007 28.169399418448883 24-25 21.923259921624634 31.564928798307214 19.87185091097958 26.639960369088573 26-27 25.390514240863943 25.34356520881532 22.164514750420178 27.10140579990055 28-29 24.715168910092505 24.8805934481482 26.61968081483967 23.784556826919626 30-31 27.109093132165114 27.73737183580233 24.351246077200308 20.80228895483225 32-33 19.953576878854925 28.29669505415014 26.614397218986035 25.135330848008902 34-35 17.599967019128908 29.756166423474383 27.750029982610087 24.893836574786622 36-37 25.88818570277722 27.269502726368632 24.499734907038498 22.342576663815645 38-39 28.1132335310539 25.018141486036804 23.159711585604203 23.708913397305096 40-41 22.359038212124307 29.193292824488598 23.7644014937573 24.683267469629797 42-43 27.383248279687834 26.38638599418284 23.49263605462842 22.737729671500908 44-45 29.206195719941704 24.400884166802346 23.105434855987834 23.287485257268116 46-47 23.557561533428803 26.36925704643981 30.285098043374205 19.788083376757186 48-49 26.412132690611124 26.089184835151613 28.30622850789875 19.192453966338515 50-51 21.691260249943774 27.584892681982144 28.104057029969297 22.619790038104785 52-53 19.64650629538452 29.006110172568956 22.46435125415816 28.883032277888365 54-55 21.835436477264604 30.46724941847178 20.731551158995437 26.965762945268175 56-57 25.25809642273377 24.53354857707503 22.612356916535976 27.59599808365522 58-59 24.589685226934876 23.784763640346707 26.71096993340066 24.914581199317755 60-61 26.88178054386415 26.372384377259074 24.6837631908026 22.06207188807418 62-63 20.220997412667653 27.327192041468212 26.564404409410198 25.88740613645394 64-65 18.089697378259327 29.095806540837554 27.615784986229674 25.198711094673442 66-67 25.2992093357454 26.60164787368412 24.751068447519632 23.348074343050847 68-69 27.91087585033598 24.456388210888697 23.254402550898046 24.378333387877273 70-71 22.618101994001023 28.079735285289324 23.852912045104905 25.449250675604745 72-73 27.070571318101422 25.762386954629648 23.58346195976017 23.583579767508763 74-75 28.621549589640434 24.036750686429855 23.07160502844985 24.270094695479862 76-77 23.807346290744388 25.905357115596217 29.306906509120328 20.980390084539064 78-79 26.485729667442513 25.938841398149826 27.377886777993975 20.197542156413682 80-81 22.256708954836064 27.20678110408642 27.210269326353092 23.326240614724426 82-83 20.306031234935954 28.425476550397573 22.655671569843356 28.612820644823117 84-85 22.19189974719172 29.672599525781767 20.91866915252469 27.216831574501825 86-87 25.12703043994839 24.390474344178596 22.863402939085812 27.619092276787207 88-89 24.718289751562743 23.689992098292656 26.433982158838116 25.157735991306485 90-91 26.636963608746868 26.06442168001837 24.596712402016475 22.701902309218287 92-93 21.272426238168734 26.8646429108839 25.773303698002874 26.089627152944487 94-95 19.279540108941468 28.217903475441485 27.115044421295426 25.387511994321617 96-97 25.439200464932814 26.28084089354859 24.72289584057846 23.557062800940134 98-99 27.38809395638792 24.38991509144393 23.47913764776897 24.74285330439918 100 22.56949285638011 27.01131871703487 26.073664689361305 24.34552373722372 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1049.0 1 1197.0 2 1198.5 3 951.5 4 752.5 5 677.0 6 725.5 7 826.0 8 989.5 9 1177.5 10 1427.0 11 1782.0 12 2285.0 13 2916.5 14 3636.5 15 4357.0 16 5023.5 17 5861.0 18 6615.0 19 6858.0 20 6967.0 21 7268.0 22 7689.0 23 8269.0 24 8992.5 25 10151.0 26 11863.0 27 13678.0 28 15615.0 29 17472.0 30 19251.5 31 20882.0 32 22238.5 33 23776.0 34 25562.5 35 27353.5 36 29259.0 37 31834.0 38 34932.5 39 37582.0 40 39500.0 41 41094.0 42 41941.5 43 42889.0 44 44799.5 45 48442.0 46 57280.5 47 75780.5 48 112677.0 49 178105.5 50 248676.0 51 242415.5 52 163194.5 53 96016.0 54 60164.0 55 42036.0 56 31871.5 57 25534.0 58 21445.0 59 18708.5 60 16319.0 61 14001.5 62 11906.0 63 9997.5 64 8220.5 65 6796.5 66 5549.0 67 4318.5 68 3371.0 69 2550.5 70 1972.5 71 1524.0 72 1133.5 73 877.5 74 667.0 75 486.5 76 357.0 77 266.0 78 192.5 79 124.0 80 87.0 81 67.5 82 47.0 83 33.0 84 20.0 85 9.5 86 5.5 87 8.0 88 8.0 89 3.5 90 1.5 91 1.5 92 1.5 93 0.5 94 0.5 95 1.5 96 1.5 97 1.0 98 0.5 99 0.5 100 1.0 >>END_MODULE >>Per base N content fail #Base N-Count 1 7.862459080401816 2 24.553198147191235 3 5.9837440497994585 4 24.17240388334009 5 12.08592337966284 6 9.667123044088473 7 7.70637150236383 8 8.659935680032536 9 4.942386412859537 10-11 6.794568412275486 12-13 9.932662277457974 14-15 7.3099777661091245 16-17 7.938483294868563 18-19 6.591311001017215 20-21 7.861182337868787 22-23 5.733131097316101 24-25 5.203468654113734 26-27 9.616285477773305 28-29 6.261679292489643 30-31 4.31265056856827 32-33 8.702369959130312 34-35 7.09214227647372 36-37 6.609371104484431 38-39 6.1107451115849765 40-41 8.610351642749798 42-43 6.390328512817798 44-45 6.470391876389038 46-47 5.869463986343034 48-49 3.9323437882297334 50-51 6.383968013653253 52-53 4.432223310161617 54-55 4.825274302329846 56-57 4.691564538870774 58-59 3.572395247917865 60-61 4.082952980125993 62-63 3.0756727620676543 64-65 4.408824101556279 66-67 2.4648327072652223 68-69 4.950975408081733 70-71 2.4789000886291452 72-73 1.477191110714934 74-75 0.8464106588966437 76-77 1.1616732105524858 78-79 1.7320753473087893 80-81 2.1741068257441434 82-83 0.7989622636691538 84-85 1.2264853042291748 86-87 0.49368151727154086 88-89 0.7323163034450229 90-91 0.32508186240986775 92-93 0.342724122866273 94-95 0.5459583206239418 96-97 0.5419888120212506 98-99 0.4736018392520796 100 0.43724949731164453 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 2153919.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 63.90276886038334 #Duplication Level Percentage of deduplicated Percentage of total 1 92.35699174772752 59.01867496295365 2 4.6094118476914625 5.8910835977068015 3 1.2902418733088534 2.4735008461213104 4 0.5414777260291558 1.3840770387794847 5 0.28190388480484113 0.9007219395763946 6 0.1713993433810085 0.6571735573738836 7 0.11875067559865914 0.5311947882356836 8 0.08236365109276844 0.4210612286622754 9 0.06160821862137201 0.35432421790153507 >10 0.39690908652946744 4.810724866936559 >50 0.04343785685541128 1.94552753821305 >100 0.036845004018479004 4.693685673261693 >500 0.0042478526955608215 1.8349467491155476 >1k 0.003349268471499879 5.182128495105389 >5k 4.901368494877871E-4 2.3265836437464325 >10k+ 5.718263244024182E-4 7.574590856310323 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 44821 2.0809046208330026 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 35169 1.6327912052403086 No Hit CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC 15447 0.7171578875528746 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 15098 0.7009548641337023 No Hit GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC 13816 0.6414354485939351 No Hit ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG 10473 0.4862299835787697 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG 10278 0.4771767183445617 No Hit ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC 9396 0.43622810328522105 No Hit TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG 8744 0.4059576985021257 No Hit GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT 7974 0.37020890757730446 No Hit GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT 7663 0.35577011020377275 No Hit GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC 5573 0.2587376776935437 No Hit CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC 5219 0.24230251926836616 No Hit ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA 4929 0.2288386889200569 No Hit CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT 4262 0.1978718791189455 No Hit GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC 4245 0.19708262009852737 No Hit TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC 4086 0.1897007269075578 No Hit GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG 4057 0.18835434387272687 No Hit CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA 4047 0.1878900738607162 No Hit CNCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 4003 0.18584728580786927 No Hit CCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA 3899 0.18101887768295835 No Hit CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTC 3810 0.17688687457606345 No Hit GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT 3669 0.17034066740671305 No Hit GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG 3596 0.16695149631903522 No Hit GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG 3452 0.16026600814608163 No Hit CTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT 3240 0.15042348389145554 No Hit GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA 3110 0.1443879737353169 No Hit ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC 2799 0.12994917636178518 No Hit GNACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 2745 0.1274421182969276 No Hit AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG 2585 0.12001379810475696 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGAAAGCAGTGGTA 2488 0.1155103789882535 No Hit CCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGA 2480 0.11513896297864498 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTTCCATGTACTCTGCGTTGAT 2362 0.10966057683691913 No Hit ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA 2311 0.10729279977566472 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 4.642700120106652E-5 0.0 0.0 0.0 6 0.0 4.642700120106652E-5 0.0 0.0 0.0 7 0.0 4.642700120106652E-5 0.0 4.642700120106652E-5 0.0 8 0.0 4.642700120106652E-5 0.0 9.285400240213305E-5 0.0 9 0.0 4.642700120106652E-5 0.0 9.285400240213305E-5 0.0 10-11 0.0 6.964050180159979E-5 0.0 2.321350060053326E-4 0.0 12-13 0.0 9.285400240213305E-5 0.0 2.785620072063991E-4 0.0 14-15 0.0 9.285400240213305E-5 0.0 3.4820250900799893E-4 0.0 16-17 0.0 9.285400240213305E-5 0.0 4.6427001201066517E-4 0.0 18-19 0.0 9.285400240213305E-5 0.0 5.803375150133315E-4 0.0 20-21 0.0 1.3928100360319955E-4 0.0 8.821130228202638E-4 0.0 22-23 0.0 1.3928100360319955E-4 0.0 0.0019035070492437272 0.0 24-25 0.0 1.3928100360319955E-4 0.0 0.0028552605738655914 0.0 26-27 0.0 1.3928100360319955E-4 0.0 0.0038534410996885213 0.0 28-29 0.0 1.3928100360319955E-4 0.0 0.007358679690369043 0.0 30-31 4.642700120106652E-5 1.3928100360319955E-4 0.0 0.015111988890947153 0.0 32-33 4.642700120106652E-5 1.3928100360319955E-4 0.0 0.0328006763485535 0.0 34-35 4.642700120106652E-5 1.3928100360319955E-4 0.0 0.06430139666347713 0.0 36-37 4.642700120106652E-5 1.3928100360319955E-4 0.0 0.10202333513934367 0.0 38-39 4.642700120106652E-5 1.3928100360319955E-4 0.0 0.17252273646316318 0.0 40-41 4.642700120106652E-5 1.3928100360319955E-4 0.0 0.3225748043450102 0.0 42-43 4.642700120106652E-5 1.3928100360319955E-4 0.0 0.4112039496378462 0.0 44-45 4.642700120106652E-5 1.624945042037328E-4 0.0 0.4900834246784582 0.0 46-47 4.642700120106652E-5 1.857080048042661E-4 0.0 0.5959602009174905 0.0 48-49 4.642700120106652E-5 1.857080048042661E-4 0.0 0.681176961622048 0.0 50-51 4.642700120106652E-5 1.857080048042661E-4 0.0 0.771895321968932 0.0 52-53 4.642700120106652E-5 1.857080048042661E-4 0.0 0.9196260397907257 0.0 54-55 4.642700120106652E-5 1.857080048042661E-4 0.0 1.0472538660924575 0.0 56-57 4.642700120106652E-5 1.857080048042661E-4 0.0 1.1664784051767962 0.0 58-59 4.642700120106652E-5 1.857080048042661E-4 0.0 1.3171108105736566 0.0 60-61 4.642700120106652E-5 1.857080048042661E-4 0.0 1.4425565678189385 0.0 62-63 4.642700120106652E-5 1.857080048042661E-4 0.0 1.574177116223962 0.0 64-65 4.642700120106652E-5 2.321350060053326E-4 0.0 1.7552424209081214 0.0 66-67 4.642700120106652E-5 2.5534850660586584E-4 0.0 1.945198496322285 0.0 68-69 4.642700120106652E-5 3.249890084074656E-4 0.0 2.197506034349481 4.642700120106652E-5 70-71 4.642700120106652E-5 3.714160096085322E-4 0.0 2.6598493258103018 4.642700120106652E-5 72-73 4.642700120106652E-5 3.714160096085322E-4 0.0 2.9780367785418114 4.642700120106652E-5 74-75 4.642700120106652E-5 3.714160096085322E-4 0.0 3.2391886602978106 4.642700120106652E-5 76-77 4.642700120106652E-5 3.714160096085322E-4 0.0 3.567520412791753 4.642700120106652E-5 78-79 4.642700120106652E-5 3.714160096085322E-4 0.0 3.8613104763921022 4.642700120106652E-5 80-81 4.642700120106652E-5 3.714160096085322E-4 0.0 4.17552842052092 4.642700120106652E-5 82-83 4.642700120106652E-5 3.714160096085322E-4 0.0 4.665518991196976 4.642700120106652E-5 84-85 4.642700120106652E-5 4.178430108095987E-4 0.0 5.093924144779818 4.642700120106652E-5 86-87 4.642700120106652E-5 4.178430108095987E-4 0.0 5.488089384976872 4.642700120106652E-5 88 4.642700120106652E-5 4.178430108095987E-4 0.0 5.892329284434559 4.642700120106652E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACAAA 895 0.0 27.024519 1 TACGACT 520 0.0 26.082314 8 GATACGA 445 0.0 25.946398 6 CTGTGCG 770 0.0 25.452349 9 TGATACG 505 0.0 23.736254 5 GAACAAA 1445 0.0 23.605394 1 ACGACTG 630 0.0 23.131908 9 TACTAAG 165 2.4331236E-4 22.547306 2 ATACGAC 540 0.0 22.158764 7 TATACGA 160 3.389748E-5 21.850954 5 AGTACTC 1590 0.0 21.046022 5 ATCGTTC 120 0.003446356 20.93025 8 ACTGTGC 1155 0.0 20.875887 8 ACGGTAC 135 0.003609142 20.76407 3 ACTAAGT 190 5.2311087E-5 20.654787 3 TAGACTG 325 2.4920155E-10 19.978016 5 GTAGACC 175 7.143439E-4 19.221598 3 CGGTACT 205 9.380111E-5 19.144053 4 TGTACTA 760 0.0 19.057974 5 TACTGTG 1115 0.0 18.780298 7 >>END_MODULE