FastQCFastQC Report
Wed 25 May 2016
SRR1295090_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295090_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2153919
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT1266395.879469005101863No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA860233.993789924319345No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC371311.723880981596801No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG355961.652615534753164No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC309311.4360335741501886No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG281711.307895050835245No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC268871.2482827812930757No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG255721.187231274713673No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG215851.002126820925021No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT196180.910804909562523No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT155580.7223112846861929No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA123230.5721199358007427No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT120020.5572168684152004No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC117360.5448672860957167No Hit
GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC109600.5088399331636891No Hit
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA108010.5014580399727195No Hit
CCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA107390.49857956589825336No Hit
TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC105600.4902691326832625No Hit
CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTC101910.47313756924006894No Hit
CTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT97520.4527561157128007No Hit
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG95840.44495637951102157No Hit
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG94200.43734235131404664No Hit
GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT92400.4289854910978546No Hit
ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC87830.40776835154896723No Hit
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG87340.405493428490115No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC76120.35340233314251834No Hit
CCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGA75670.3513131180884704No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG70510.32735678546872005No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTTCCATGTACTCTGCGTTGAT64570.29977914675528655No Hit
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG60660.2816261892856695No Hit
GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA50910.23635986311462964No Hit
ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA47260.2194140076762404No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGAAAGCAGTGGTA45170.20971076442521747No Hit
TTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTG41250.1915113799543994No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT36330.16866929536347466No Hit
CCCATGTACTCTGCGTTGATACCACTGCTCCCCATGTACTCTGCGTTGAT34130.15845535509924005No Hit
GGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC33450.15529831901756752No Hit
CTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGC31990.1485199768422118No Hit
CTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTT31530.14638433478696275No Hit
TCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGA30320.1407666676416337No Hit
TGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATG28850.13394189846507693No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT28800.1337097634590716No Hit
GTACATGGAAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA28410.13189911041223No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27690.1285563663257532No Hit
GCTTTCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT25570.11871384207112709No Hit
TCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGA23840.11068197086334258No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGGAGCAGTGGTA23180.10761778878407219No Hit
GTACATGGGGAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA23110.10729279977566472No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTTCCCATGTACTCTGCGTTGA21820.10130371662072715No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAGTAC16300.028.8460043
AGTACTC20700.028.1666185
AAAAGTA16800.027.7916472
TATACGT700.00881930926.868624
AAGTACT20950.026.7083054
TAGGCGT1101.0157873E-425.6473184
CCCGAAC1151.3739808E-424.5316473
AAAAAGT19950.022.673841
GTACAAA7350.021.6415941
GTATAGG2751.6860213E-819.8832151
CCTTATA2903.1290256E-818.840412
GTACAAG7700.018.075651
CTAGACA2957.1266186E-817.5329114
GTGTGCG1650.001582851317.0731969
GTATATA3802.1409505E-917.005381
CGTCTTC12300.016.78856392-93
ATGTACC45100.016.14787994
GTATAAG3702.8368959E-816.1215251
GAGTGCG2053.6408962E-416.0328927
GTCTTCT18050.015.35801594
Produced by FastQC (version 0.11.5)