FastQCFastQC Report
Wed 25 May 2016
SRR1295089_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295089_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3424937
Sequences flagged as poor quality0
Sequence length100
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATATA25350.018.9573021
TACACCG3008.1684266E-716.7477175
TACCGTG3601.960143E-816.6132417
GTATTAG13200.016.0756571
GTACTGG13850.015.7718281
GTCTCGC4804.242611E-815.6028441
TAGCGCT2907.088837E-515.5728184
GTACATG142400.015.5151891
TAATACT19150.015.3288574
TCTATAC12400.015.0239673
TACATGG145150.015.0029852
TACACTG19700.014.7923995
GTATAGG9350.014.6850291
CTGTGCG5601.0004442E-1014.3634919
CATGGGG39300.014.2205984
GTCCTAA10550.014.1978481
GTCTAGG9750.014.0825681
AGTACCG2509.819192E-414.0680815
ACATGGG148450.013.9565533
GTATAGA11750.013.8101771
Produced by FastQC (version 0.11.5)