##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1295089_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3424937 Sequences flagged as poor quality 0 Sequence length 100 %GC 42 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 25.91319898730984 27.0 22.0 33.0 6.0 33.0 2 21.980534532460013 27.0 2.0 33.0 2.0 33.0 3 24.320065741355243 27.0 15.0 33.0 15.0 33.0 4 24.402829015541016 33.0 6.0 37.0 2.0 37.0 5 26.166170647810457 33.0 15.0 37.0 2.0 37.0 6 27.058990574133187 33.0 15.0 37.0 2.0 37.0 7 26.82113627199566 33.0 15.0 37.0 6.0 37.0 8 27.67561242732348 33.0 15.0 37.0 6.0 37.0 9 28.713148884198453 33.0 22.0 37.0 15.0 37.0 10-11 28.257381960602487 33.0 18.5 37.0 6.0 37.0 12-13 27.105904867739174 33.0 15.0 37.0 4.0 37.0 14-15 28.773303713323777 33.0 22.0 37.0 6.0 40.0 16-17 29.170680073823256 33.0 22.0 37.0 6.0 40.0 18-19 29.367309238096936 33.0 22.0 38.5 6.0 40.0 20-21 28.846764772607496 33.0 22.0 37.0 6.0 40.0 22-23 29.573343392885768 33.0 24.5 37.0 6.0 40.0 24-25 29.750423146469554 33.0 24.5 37.0 6.0 40.0 26-27 28.309631388840145 33.0 18.5 37.0 4.0 40.0 28-29 29.02731188924059 33.0 22.0 37.0 6.0 40.0 30-31 29.73211945212423 33.0 27.0 37.0 10.5 40.0 32-33 28.52001350681779 33.0 22.0 37.0 6.0 40.0 34-35 28.12136237834448 33.0 18.5 37.0 6.0 40.0 36-37 28.421431255523824 33.0 22.0 37.0 6.0 40.0 38-39 28.54252224201496 33.0 22.0 37.0 6.0 40.0 40-41 27.574170123421247 33.0 15.0 37.0 6.0 40.0 42-43 28.193855828588966 33.0 22.0 37.0 6.0 40.0 44-45 28.056967179250304 33.0 22.0 37.0 6.0 40.0 46-47 27.807078203190308 33.0 22.0 37.0 6.0 40.0 48-49 27.744890927920718 33.0 18.5 37.0 6.0 40.0 50-51 27.70336111291974 33.0 22.0 37.0 6.0 40.0 52-53 27.88291156888433 33.0 22.0 37.0 6.0 40.0 54-55 27.86749770871698 33.0 22.0 37.0 6.0 40.0 56-57 27.835136529518646 33.0 22.0 37.0 6.0 40.0 58-59 27.767885365482634 33.0 22.0 37.0 6.0 40.0 60-61 27.727271333750082 33.0 22.0 37.0 6.0 40.0 62-63 27.704806686955116 33.0 22.0 37.0 6.0 40.0 64-65 27.248436686572628 33.0 22.0 37.0 6.0 40.0 66-67 27.42225959192826 33.0 22.0 37.0 6.0 38.5 68-69 27.0272156830914 33.0 22.0 37.0 6.0 37.0 70-71 27.150798394247836 33.0 22.0 37.0 6.0 37.0 72-73 27.225332173993273 27.0 22.0 37.0 10.5 37.0 74-75 27.314847543181088 27.0 22.0 37.0 6.0 37.0 76-77 27.082112021330612 27.0 22.0 37.0 6.0 37.0 78-79 26.684155796150414 27.0 22.0 33.0 6.0 37.0 80-81 26.44298114096697 27.0 22.0 33.0 6.0 37.0 82-83 26.595491391520486 27.0 22.0 33.0 10.5 37.0 84-85 26.548768342308193 27.0 22.0 33.0 6.0 37.0 86-87 26.314620385718044 27.0 22.0 33.0 10.5 37.0 88-89 26.295135063798256 27.0 22.0 33.0 10.5 37.0 90-91 26.43727052497608 27.0 22.0 33.0 15.0 37.0 92-93 26.351142809342186 27.0 22.0 33.0 10.5 37.0 94-95 26.223224252008137 27.0 22.0 33.0 10.5 37.0 96-97 26.29494746910673 27.0 22.0 33.0 10.5 37.0 98-99 26.35407030844655 27.0 22.0 33.0 10.5 37.0 100 26.029930769529482 27.0 22.0 33.0 6.0 37.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 7.257019033158251 1101 2 11.193601181890148 1101 3 8.312165570536898 1101 4 12.733011752866052 1101 5 10.66273206449915 1101 6 9.912983228206347 1101 7 9.441111046945377 1101 8 8.934761010710343 1101 9 7.344982747538101 1101 10-11 7.766871598526489 1101 12-13 9.03350081103897 1101 14-15 7.870713748569564 1101 16-17 8.083801825463425 1101 18-19 8.081662044297929 1101 20-21 7.204082805317761 1101 22-23 7.278876797508222 1101 24-25 7.224824229933951 1101 26-27 8.386764095132524 1101 28-29 8.107905131128057 1101 30-31 5.507332538378542 1101 32-33 6.916306982803366 1101 34-35 8.5873775411278 1101 36-37 8.09229197038843 1101 38-39 8.137646268629872 1101 40-41 8.950909110352093 1101 42-43 8.443326842876054 1101 44-45 8.349052852948208 1101 46-47 7.191724909690027 1101 48-49 5.233116163859737 1101 50-51 6.728646362093347 1101 52-53 5.725634886795827 1101 54-55 4.067619854660268 1101 56-57 6.062750907655094 1101 58-59 6.141796275686875 1101 60-61 6.657828922717531 1101 62-63 6.118380852364563 1101 64-65 6.6301530752471045 1101 66-67 5.668931232513625 1101 68-69 6.740009074721392 1101 70-71 6.252232426213762 1101 72-73 5.40570260555338 1101 74-75 4.782465584062262 1101 76-77 5.005628454089642 1101 78-79 5.058510607505866 1101 80-81 4.85895247634663 1101 82-83 4.389474269495587 1101 84-85 4.094480933173855 1101 86-87 4.064146617736384 1101 88-89 4.038956086466403 1101 90-91 3.790910609613274 1101 92-93 3.6774976305761804 1101 94-95 3.4748301242659814 1101 96-97 3.6580460899075398 1101 98-99 3.428605392082819 1101 100 3.7100812702535038 1102 1 6.794209636577282 1102 2 10.402910964280903 1102 3 8.058073180107055 1102 4 12.475843026014847 1102 5 10.335616319308148 1102 6 9.372015251602253 1102 7 6.465066246663675 1102 8 7.555524637232448 1102 9 6.297854184092248 1102 10-11 7.289400628501195 1102 12-13 8.861966773954443 1102 14-15 6.068990484132399 1102 16-17 7.180594657307978 1102 18-19 7.278365898184298 1102 20-21 6.922718447915003 1102 22-23 6.440825872481046 1102 24-25 6.855425469957865 1102 26-27 8.297070334829531 1102 28-29 7.477675504161901 1102 30-31 4.156121900666264 1102 32-33 4.425664060893023 1102 34-35 5.129857716094108 1102 36-37 5.100586391362139 1102 38-39 4.593719062282915 1102 40-41 6.285040732218349 1102 42-43 6.811270032928341 1102 44-45 6.3083212802662025 1102 46-47 5.404976795761918 1102 48-49 3.7630262516954325 1102 50-51 4.92052360871595 1102 52-53 5.697716059929011 1102 54-55 5.3348790607991 1102 56-57 6.276223910301095 1102 58-59 5.874655891392404 1102 60-61 5.966391839858222 1102 62-63 4.302369137632514 1102 64-65 6.047023096267395 1102 66-67 3.8089827381990204 1102 68-69 5.91577111685903 1102 70-71 5.212587345860388 1102 72-73 4.865695988741738 1102 74-75 4.165467818606455 1102 76-77 3.3066860197729895 1102 78-79 4.343429534318378 1102 80-81 4.334214433909807 1102 82-83 3.7821276398608425 1102 84-85 2.930087830728322 1102 86-87 3.2327722648316453 1102 88-89 3.37926708742658 1102 90-91 3.259851239463572 1102 92-93 3.121443502402169 1102 94-95 3.0814675700991323 1102 96-97 3.1670602466815687 1102 98-99 3.0434648210962685 1102 100 3.089478552390478 1103 1 6.58131179209818 1103 2 10.781171482144739 1103 3 7.730324117522102 1103 4 11.76998567499803 1103 5 10.077825659215964 1103 6 8.94577323778267 1103 7 7.855900757330275 1103 8 7.9004864224444695 1103 9 7.041238718633377 1103 10-11 7.379287953284017 1103 12-13 8.62217015466129 1103 14-15 8.355453300534414 1103 16-17 7.788988984635253 1103 18-19 7.081104752315163 1103 20-21 8.18186250660792 1103 22-23 6.796403677388415 1103 24-25 5.212041022092734 1103 26-27 5.849770311948948 1103 28-29 6.851344992264195 1103 30-31 6.038799446225859 1103 32-33 7.815352140167793 1103 34-35 7.0695141407291615 1103 36-37 4.179423185903602 1103 38-39 6.268919178790526 1103 40-41 6.677434525244621 1103 42-43 7.284467779375007 1103 44-45 5.434431277119675 1103 46-47 6.126867039490868 1103 48-49 5.343783842878253 1103 50-51 6.157763097644871 1103 52-53 5.9001019807019865 1103 54-55 4.694064471480058 1103 56-57 5.777499733883705 1103 58-59 5.309717621378621 1103 60-61 4.765146471583304 1103 62-63 2.663182766355387 1103 64-65 4.853718672017326 1103 66-67 4.0072010226176005 1103 68-69 5.0007243727759985 1103 70-71 3.5298741821184407 1103 72-73 4.145475379107616 1103 74-75 3.1464799526997282 1103 76-77 3.3570787289347486 1103 78-79 2.797004079793684 1103 80-81 3.8506998695440338 1103 82-83 3.2365198603207155 1103 84-85 2.9677691279454272 1103 86-87 2.4750868689704006 1103 88-89 2.7252590262949816 1103 90-91 2.3616237655049517 1103 92-93 2.0487927832523596 1103 94-95 2.59446456061427 1103 96-97 2.777141288928032 1103 98-99 2.5190789907301294 1103 100 2.6354810374667075 1104 1 6.21709431751426 1104 2 9.619377943280291 1104 3 7.096092552299826 1104 4 11.966541963037852 1104 5 9.21273647597042 1104 6 7.858507299921893 1104 7 3.797502326845855 1104 8 6.346286261795488 1104 9 6.393048682352134 1104 10-11 6.951156410467025 1104 12-13 7.244789991180454 1104 14-15 6.986994164125285 1104 16-17 6.9668115871054965 1104 18-19 6.608712441163341 1104 20-21 7.263490340035624 1104 22-23 6.22789196422524 1104 24-25 5.745097060761374 1104 26-27 7.952281555082802 1104 28-29 6.979950969973537 1104 30-31 6.232310358116486 1104 32-33 7.381808461256075 1104 34-35 7.917512939332411 1104 36-37 6.471056643221452 1104 38-39 7.158714543456391 1104 40-41 7.045400788958759 1104 42-43 7.15502861098097 1104 44-45 6.575926667119024 1104 46-47 6.384114800887115 1104 48-49 5.336685744020151 1104 50-51 5.461774717443195 1104 52-53 5.804719863216125 1104 54-55 4.832342159580637 1104 56-57 5.855457814945783 1104 58-59 3.75658925046298 1104 60-61 4.101901965648331 1104 62-63 3.6139513299056674 1104 64-65 2.689100604783789 1104 66-67 3.1738877953774285 1104 68-69 4.0596828311283915 1104 70-71 2.4718305683895316 1104 72-73 2.3372510950157057 1104 74-75 2.9340937528393347 1104 76-77 3.5567260135046155 1104 78-79 4.091210352113389 1104 80-81 2.8117920189962113 1104 82-83 2.687374563039498 1104 84-85 2.752921466259725 1104 86-87 0.6920986397682967 1104 88-89 0.3362504922967666 1104 90-91 2.0703202523051196 1104 92-93 2.222466472379022 1104 94-95 2.307163105044385 1104 96-97 2.625971400677958 1104 98-99 2.263461899550851 1104 100 0.7643615814050229 1105 1 5.754930332101317 1105 2 -2.6931421385718153 1105 3 0.5011400926290257 1105 4 8.315993863393981 1105 5 8.483278771701475 1105 6 7.909891341452685 1105 7 5.115301580041969 1105 8 5.818030559513531 1105 9 4.912119121748635 1105 10-11 4.962893701298672 1105 12-13 5.0726860092736 1105 14-15 5.716140747364612 1105 16-17 5.953375322910873 1105 18-19 6.047633508062553 1105 20-21 6.990762229571047 1105 22-23 2.208599218560515 1105 24-25 4.0901421441058545 1105 26-27 6.806371652058278 1105 28-29 5.925263032551147 1105 30-31 5.3082956348841535 1105 32-33 7.157203188383139 1105 34-35 6.248157007309722 1105 36-37 6.306324881947518 1105 38-39 4.331738287894293 1105 40-41 5.453936319456687 1105 42-43 5.588318234131538 1105 44-45 6.056148856946063 1105 46-47 5.421513367095333 1105 48-49 5.113028757989198 1105 50-51 5.956685859394419 1105 52-53 5.496876993343051 1105 54-55 5.269845536091598 1105 56-57 5.23447605201785 1105 58-59 2.1165073613059953 1105 60-61 4.385575491242957 1105 62-63 -0.6368648075276013 1105 64-65 -0.16608832085758252 1105 66-67 0.9555371345491039 1105 68-69 2.86892264557709 1105 70-71 3.028984856859953 1105 72-73 2.719209991956216 1105 74-75 2.745652391507239 1105 76-77 1.8357780241925568 1105 78-79 3.244621855920734 1105 80-81 1.9212032704134963 1105 82-83 1.1208066470710207 1105 84-85 1.3953737464485272 1105 86-87 -0.13234128661516564 1105 88-89 1.685398766579734 1105 90-91 1.4890020352057363 1105 92-93 1.8294697067598982 1105 94-95 1.193451742868092 1105 96-97 2.1595425204047913 1105 98-99 2.0623362459348904 1105 100 2.119732682690298 1106 1 6.111500432048285 1106 2 10.209599503106787 1106 3 6.738304175688587 1106 4 11.733575653313313 1106 5 9.844766901841144 1106 6 9.41394522983948 1106 7 6.593091023517442 1106 8 7.273211719503799 1106 9 6.883863575264787 1106 10-11 5.692192961072454 1106 12-13 7.946034784271397 1106 14-15 7.76391488849378 1106 16-17 7.6443373414615365 1106 18-19 7.153697152381618 1106 20-21 8.173053145056095 1106 22-23 6.097320489194221 1106 24-25 5.046162852679231 1106 26-27 7.552964285243096 1106 28-29 6.2013535423131785 1106 30-31 5.422383716414494 1106 32-33 7.397276215919636 1106 34-35 6.808516046021893 1106 36-37 5.988487162403899 1106 38-39 7.05107361555617 1106 40-41 6.607677472715004 1106 42-43 6.143551447604715 1106 44-45 4.4179504343192555 1106 46-47 5.843487877085607 1106 48-49 5.763997886420089 1106 50-51 6.616971407003668 1106 52-53 5.649047280094258 1106 54-55 5.130906244955408 1106 56-57 5.064909522928641 1106 58-59 5.030451616233275 1106 60-61 4.89130403661154 1106 62-63 4.922418738461399 1106 64-65 5.1970615459787695 1106 66-67 3.0174244810194857 1106 68-69 3.3111141065159835 1106 70-71 2.9451441866881147 1106 72-73 2.7906784000439835 1106 74-75 2.8409313398109255 1106 76-77 3.5509337606362408 1106 78-79 4.142794938778021 1106 80-81 3.599265315149143 1106 82-83 2.0730764702837305 1106 84-85 2.815420955755375 1106 86-87 3.5093344427130546 1106 88-89 3.33944161966539 1106 90-91 3.1862037974656054 1106 92-93 2.836432102121183 1106 94-95 1.4945894912589424 1106 96-97 1.5021243359226695 1106 98-99 2.009634114450879 1106 100 2.9523099900712317 1107 1 6.005715390370554 1107 2 10.31297311304023 1107 3 6.0579670942713015 1107 4 11.442418904119695 1107 5 9.276915715068636 1107 6 8.079169265466945 1107 7 3.588787967156673 1107 8 6.192659132315313 1107 9 5.697660328671553 1107 10-11 4.565308973712263 1107 12-13 6.104187356821615 1107 14-15 6.113493801437045 1107 16-17 6.989711523364861 1107 18-19 6.232449817758521 1107 20-21 5.571771014823987 1107 22-23 5.754284696306765 1107 24-25 4.468636625240485 1107 26-27 7.168100598077007 1107 28-29 6.4046862003913745 1107 30-31 5.087766023671186 1107 32-33 5.005227228992748 1107 34-35 6.754354110553063 1107 36-37 6.467464324569434 1107 38-39 6.425442611927345 1107 40-41 7.628395916310446 1107 42-43 6.216554552385084 1107 44-45 4.308702587728309 1107 46-47 3.604342046033697 1107 48-49 4.123150756101143 1107 50-51 4.852155728898442 1107 52-53 3.878183498745397 1107 54-55 5.141757864281303 1107 56-57 5.265485319574118 1107 58-59 3.1210046018873037 1107 60-61 2.8994980022084533 1107 62-63 0.5145158477657006 1107 64-65 2.1956824372208033 1107 66-67 4.0430362858538516 1107 68-69 4.932669151280198 1107 70-71 4.187921292771257 1107 72-73 2.417390880031302 1107 74-75 2.5766541567747048 1107 76-77 3.093270102523764 1107 78-79 3.940322585460013 1107 80-81 2.198380628755924 1107 82-83 1.6535138275524908 1107 84-85 2.3365489922091243 1107 86-87 2.358080887754859 1107 88-89 2.526983208926179 1107 90-91 1.3668808006443172 1107 92-93 2.209749335217303 1107 94-95 2.1532932466155863 1107 96-97 2.206736096266358 1107 98-99 1.7734430029447061 1107 100 0.12193491510351606 1108 1 4.266211612013802 1108 2 -3.1594350416211814 1108 3 0.09241590283003731 1108 4 6.487469318063749 1108 5 6.831289164153276 1108 6 6.688914997954807 1108 7 4.208833646225475 1108 8 4.945338094041084 1108 9 4.763753198051109 1108 10-11 3.2981186431829883 1108 12-13 2.411534635008593 1108 14-15 3.9339720067656785 1108 16-17 4.478535995685384 1108 18-19 3.803889490559474 1108 20-21 4.725070994783682 1108 22-23 4.077326143215235 1108 24-25 2.9149062627985884 1108 26-27 4.663475439833473 1108 28-29 6.083269322719339 1108 30-31 4.498436479954574 1108 32-33 4.524767799349913 1108 34-35 3.12549872753398 1108 36-37 1.326904671363625 1108 38-39 2.564201025484884 1108 40-41 5.3475504313122855 1108 42-43 3.2023617183377446 1108 44-45 3.6420916984611758 1108 46-47 4.7682247030723595 1108 48-49 4.881112991545876 1108 50-51 2.120640460789197 1108 52-53 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-23.10893323585342 2213 44-45 -16.997613380761194 2213 46-47 -16.840529849053834 2213 48-49 -6.109228882268329 2213 50-51 -23.23714704092009 2213 52-53 -17.68656553766953 2213 54-55 -17.607994672895558 2213 56-57 -17.340730445033472 2213 58-59 -17.89468088484188 2213 60-61 -19.163400421341997 2213 62-63 -17.468724412089177 2213 64-65 -17.55355703310649 2213 66-67 -8.675454055041072 2213 68-69 -22.681193585791707 2213 70-71 -17.35447476934086 2213 72-73 -9.457173836154823 2213 74-75 -4.304854927975672 2213 76-77 -3.0561220060668184 2213 78-79 -4.836096189718784 2213 80-81 -3.503638129948701 2213 82-83 -3.353369323206092 2213 84-85 -3.0879851430914727 2213 86-87 -3.3718898229572503 2213 88-89 -2.9255849690778284 2213 90-91 -2.821263089496476 2213 92-93 -3.6344296359481305 2213 94-95 -3.6661429443985334 2213 96-97 -3.2868901775753194 2213 98-99 -3.594008820603438 2213 100 -3.1191543920089835 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1000.0 3 962.0 4 4682.0 5 15713.0 6 24374.0 7 31570.0 8 30749.0 9 32772.0 10 37043.0 11 44055.0 12 55074.0 13 73835.0 14 61836.0 15 67121.0 16 74106.0 17 77764.0 18 76945.0 19 75568.0 20 73997.0 21 71334.0 22 73794.0 23 78767.0 24 85650.0 25 93238.0 26 101569.0 27 110228.0 28 117913.0 29 122940.0 30 127687.0 31 139577.0 32 156333.0 33 188552.0 34 227187.0 35 265857.0 36 289037.0 37 243418.0 38 72511.0 39 179.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.40615311255812 21.566333762465582 13.745279040613717 24.282234084362575 2 14.47108623562893 22.46332318347832 40.28538973366524 22.780200847227505 3 17.732239696354615 27.707049146659614 30.11543772748394 24.44527342950183 4 11.75097623476967 18.19375714111127 39.77160083103621 30.283665793082847 5 12.758877601361215 38.02630474982297 35.871449714567625 13.343367934248192 6 29.387877547436315 38.96291827801776 19.282874092151165 12.366330082394759 7 25.002377471178526 32.19458559961731 24.92254532859514 17.880491600609012 8 23.892514597065883 36.59554170835083 22.43743691315823 17.074506781425054 9 24.249533614583736 17.184998258311722 22.846955915934515 35.71851221117003 10-11 22.661706140577845 27.956847009252932 29.56149425441031 19.81995259575891 12-13 23.453350423891386 25.699380431941517 29.776378602930038 21.07089054123706 14-15 21.093268770104714 26.921354915246503 27.01332329523418 24.9720530194146 16-17 20.597110589762906 30.730100043531817 27.926288039168117 20.74650132753716 18-19 20.452185967977695 29.7266089430612 29.49740675435625 20.323798334604852 20-21 21.982653909127272 29.141866813255145 28.287650526930047 20.58782875068753 22-23 20.798193341987957 29.352817216943755 28.932426814255148 20.916562626813135 24-25 21.24365907674149 28.916448511355537 28.31130408854853 21.52858832335445 26-27 21.246082071813728 28.998168848224033 28.472333318298183 21.28341576166406 28-29 20.90019447816951 28.779895102683255 29.13378570476899 21.18612471437825 30-31 21.757395323021893 29.321229047718163 28.121054470202168 20.800321159057784 32-33 20.508041117087128 29.26178241830579 29.08365849102096 21.14651797358613 34-35 20.699611792101354 28.526161166725533 28.84093116164477 21.933295879528337 36-37 21.448646643018275 29.322445876114937 28.282600163913465 20.946307316953327 38-39 21.119369712760566 28.91841899475952 28.622351532826396 21.33985975965352 40-41 21.397542136524752 28.97584735204996 28.08185376596446 21.54475674546083 42-43 20.94518576220764 29.15076888271317 28.87510197482164 21.02894338025755 44-45 21.581599280231167 28.297760496419954 28.664317560404694 21.456322662944185 46-47 21.2964854392731 29.047076165152525 28.84907755732538 20.807360838248997 48-49 21.065850215378262 28.7140968342616 28.9989178173074 21.22113513305274 50-51 21.54540878833731 28.76638555823427 28.451539314384235 21.236666339044188 52-53 20.673393343487483 28.94965877305476 28.842036032154976 21.53491185130278 54-55 21.318647145627427 28.68142990461796 28.419258739655234 21.580664210099375 56-57 21.461899126176487 28.819011717371197 28.36362070384204 21.355468452610282 58-59 21.009984668078275 28.2812961149546 28.96534501364435 21.743374203322773 60-61 21.69626895954201 28.815899145655344 28.186854230108853 21.30097766469379 62-63 20.877487773543614 28.996481504245814 28.89562378428409 21.230406937926478 64-65 20.963026981792463 28.26206184433347 28.843346689989126 21.931564483884944 66-67 21.235974804597426 28.75154166359961 28.46754507065739 21.54493846114557 68-69 21.62063712062949 28.504466374360284 28.491471211243237 21.38342529376699 70-71 21.803091695120642 28.655873062363867 28.044805331601587 21.496229910913904 72-73 21.265762734794436 28.8880819128181 28.72799210112834 21.11816325125912 74-75 21.72795282845034 28.144433388612494 28.314354155150173 21.81325962778699 76-77 21.592965115686802 28.85023631651842 28.422573767618914 21.134224800175865 78-79 21.501530377332617 28.489666665824753 28.67129729434993 21.337505662492703 80-81 21.788564527238492 28.496309986566942 28.226481635526124 21.488643850668442 82-83 20.99605878138521 28.85797342803635 28.799127312100676 21.34684047847777 84-85 21.42295757760558 28.250434482518173 28.2684135789009 22.058194360975346 86-87 21.67154504005942 28.70710383155373 28.298553515805047 21.3227976125818 88-89 21.33580427007838 28.15867468472419 28.73938889121488 21.766132153982547 90-91 21.89549229945646 28.31936388932496 28.15987682290527 21.625266988313303 92-93 21.129789358352888 28.680474588312222 28.721413123260277 21.46832293007461 94-95 21.213083886867974 28.0730182745339 28.54474180135761 22.169156037240512 96-97 21.530131133589162 28.493626068248417 28.41286479267823 21.563378005484193 98-99 21.430195430030558 28.1414432767094 28.545044310303552 21.88331698295649 100 21.68138170358464 28.010009780074313 28.15146633726548 22.157142179075564 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 408.0 1 478.5 2 474.0 3 400.0 4 461.5 5 639.0 6 903.5 7 1332.0 8 1937.5 9 2717.5 10 3770.0 11 4974.5 12 6201.0 13 7471.0 14 8736.5 15 9926.0 16 11116.5 17 12328.0 18 13534.0 19 14613.0 20 15796.5 21 17319.5 22 19180.5 23 21586.0 24 24520.5 25 28325.5 26 33548.0 27 40230.0 28 49064.0 29 59199.5 30 69449.5 31 80338.5 32 91269.5 33 101440.5 34 110379.5 35 118028.5 36 124962.5 37 130463.0 38 134501.0 39 137379.5 40 138346.0 41 136721.5 42 132711.0 43 127558.0 44 121420.0 45 113942.0 46 107051.5 47 100763.0 48 95334.5 49 93068.0 50 92378.0 51 87085.0 52 76361.5 53 67198.5 54 60447.5 55 54516.5 56 49268.5 57 43844.5 58 38650.0 59 33734.5 60 28947.0 61 24234.0 62 20007.0 63 16209.5 64 12806.5 65 10158.0 66 7984.5 67 6056.0 68 4585.0 69 3597.5 70 2739.0 71 2033.5 72 1550.5 73 1173.0 74 874.5 75 687.5 76 506.5 77 346.5 78 243.0 79 174.5 80 114.0 81 84.5 82 65.0 83 43.0 84 34.0 85 26.0 86 15.5 87 11.0 88 8.5 89 4.5 90 2.0 91 2.0 92 2.0 93 2.5 94 3.0 95 1.0 96 0.5 97 1.0 98 0.5 99 0.0 100 1.5 >>END_MODULE >>Per base N content fail #Base N-Count 1 8.397176356820578 2 25.48508191537538 3 6.31234968701614 4 24.742995272613776 5 13.000151535634085 6 10.55534744142739 7 8.199858858717693 8 8.854119068467536 9 5.702645041354045 10-11 7.1857526138437 12-13 10.468119559571461 14-15 7.853020362126369 16-17 8.346241113340188 18-19 6.65641440995849 20-21 8.536157015442912 22-23 6.032782500816803 24-25 5.5043202254523225 26-27 10.02266611035473 28-29 6.534616549151123 30-31 4.468344965177462 32-33 9.11597789973947 34-35 7.621731436227878 36-37 7.302061322587831 38-39 6.450848584952073 40-41 9.183001030383917 42-43 6.8201400492914175 44-45 6.797234518474355 46-47 6.02135163362129 48-49 4.125491943355454 50-51 6.721379108579223 52-53 4.7751097319454345 54-55 5.264359607198614 56-57 5.080224833332701 58-59 3.5437732139306504 60-61 4.15835386169147 62-63 3.0921736662601385 64-65 4.679575127951258 66-67 2.8190883511141953 68-69 5.2971047350652 70-71 2.7053052362714993 72-73 1.5862773534228511 74-75 0.9653316250780671 76-77 1.117436612702657 78-79 1.7399006171500382 80-81 2.4395631218910014 82-83 0.7662038746990091 84-85 1.1810728197336184 86-87 0.5473677326035487 88-89 0.6239384841239416 90-91 0.2166025243676015 92-93 0.29377182704382593 94-95 0.5554116761855765 96-97 0.543119479278013 98-99 0.47443208444418106 100 0.3467217061218936 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 3424937.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 95.7999982606969 #Duplication Level Percentage of deduplicated Percentage of total 1 97.50186127785808 93.40678140833514 2 2.0016881412337524 3.835234408973022 3 0.276639843518581 0.7950628958375856 4 0.09580936647312882 0.36714148565936855 5 0.043065197755198334 0.20628229350222843 6 0.022295306137238957 0.12815341735015182 7 0.016643056795905928 0.11160833684703271 8 0.00860662855040545 0.06596120001274451 9 0.006208916966876036 0.053533281116478204 >10 0.025163656273194075 0.3973158342590536 >50 7.560183686762592E-4 0.047358683624804124 >100 9.561790066234464E-4 0.21673149269543832 >500 1.5320553152517496E-4 0.10071418915457077 >1k 1.5320553086217177E-4 0.26812107263240625 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 8.759285207289944E-5 0.0 8 0.0 0.0 0.0 1.751857041457989E-4 0.0 9 0.0 0.0 0.0 1.751857041457989E-4 0.0 10-11 0.0 0.0 0.0 1.751857041457989E-4 1.4598808678816573E-5 12-13 0.0 0.0 0.0 2.0438332150343204E-4 2.9197617357633146E-5 14-15 0.0 0.0 0.0 2.189821301822486E-4 2.9197617357633146E-5 16-17 0.0 0.0 0.0 2.6277855621869835E-4 4.3796426036449716E-5 18-19 0.0 0.0 0.0 3.0657498225514804E-4 5.839523471526629E-5 20-21 0.0 0.0 0.0 4.379642603644972E-4 5.839523471526629E-5 22-23 0.0 0.0 0.0 4.817606864009469E-4 5.839523471526629E-5 24-25 0.0 0.0 0.0 5.83952347152663E-4 5.839523471526629E-5 26-27 0.0 2.9197617357633146E-5 0.0 6.86144007904379E-4 5.839523471526629E-5 28-29 1.4598808678816573E-5 2.9197617357633146E-5 0.0 0.0012554975463782252 5.839523471526629E-5 30-31 5.839523471526629E-5 2.9197617357633146E-5 0.0 0.003021953396515031 8.759285207289944E-5 32-33 5.839523471526629E-5 2.9197617357633146E-5 0.0 0.007591380512984618 8.759285207289944E-5 34-35 8.759285207289944E-5 2.9197617357633146E-5 0.0 0.014773994382962372 8.759285207289944E-5 36-37 8.759285207289944E-5 2.9197617357633146E-5 0.0 0.0263362508565851 8.759285207289944E-5 38-39 8.759285207289944E-5 2.9197617357633146E-5 0.0 0.044920534304718596 8.759285207289944E-5 40-41 8.759285207289944E-5 2.9197617357633146E-5 0.0 0.0695779221632398 8.759285207289944E-5 42-43 8.759285207289944E-5 2.9197617357633146E-5 0.0 0.0937243517180024 8.759285207289944E-5 44-45 1.1679046943053258E-4 2.9197617357633146E-5 0.0 0.12127230369492928 8.759285207289944E-5 46-47 1.1679046943053258E-4 2.9197617357633146E-5 0.0 0.1555503064727906 8.759285207289944E-5 48-49 1.1679046943053258E-4 2.9197617357633146E-5 0.0 0.19006189018951297 8.759285207289944E-5 50-51 1.1679046943053258E-4 2.9197617357633146E-5 0.0 0.23076336878605358 8.759285207289944E-5 52-53 1.1679046943053258E-4 2.9197617357633146E-5 0.0 0.27415102817949644 1.1679046943053258E-4 54-55 1.1679046943053258E-4 2.9197617357633146E-5 0.0 0.32199132421997834 1.1679046943053258E-4 56-57 1.1679046943053258E-4 2.9197617357633146E-5 0.0 0.3759193234795268 1.1679046943053258E-4 58-59 1.1679046943053258E-4 2.9197617357633146E-5 0.0 0.4370883318437682 1.1679046943053258E-4 60-61 1.1679046943053258E-4 2.9197617357633146E-5 0.0 0.5019362399950715 1.1679046943053258E-4 62-63 1.3138927810934917E-4 2.9197617357633146E-5 0.0 0.5698498979689262 1.1679046943053258E-4 64-65 1.4598808678816574E-4 2.9197617357633146E-5 0.0 0.6481579077220982 1.1679046943053258E-4 66-67 1.4598808678816574E-4 4.3796426036449716E-5 0.0 0.742247229657071 1.4598808678816574E-4 68-69 1.4598808678816574E-4 5.839523471526629E-5 0.0 0.8455192022510195 1.4598808678816574E-4 70-71 1.4598808678816574E-4 5.839523471526629E-5 0.0 0.9665141285810512 1.4598808678816574E-4 72-73 1.751857041457989E-4 5.839523471526629E-5 0.0 1.0914799308717211 1.4598808678816574E-4 74-75 1.751857041457989E-4 5.839523471526629E-5 0.0 1.2261393421251252 1.4598808678816574E-4 76-77 1.751857041457989E-4 5.839523471526629E-5 0.0 1.3766968560297606 1.4598808678816574E-4 78-79 1.751857041457989E-4 5.839523471526629E-5 0.0 1.5432400654377 1.4598808678816574E-4 80-81 1.751857041457989E-4 5.839523471526629E-5 0.0 1.7314479069250033 1.4598808678816574E-4 82-83 1.751857041457989E-4 5.839523471526629E-5 0.0 1.9401670746060438 1.4598808678816574E-4 84-85 1.751857041457989E-4 5.839523471526629E-5 0.0 2.153280483699408 1.4598808678816574E-4 86-87 1.751857041457989E-4 5.839523471526629E-5 0.0 2.3880731236808153 1.4598808678816574E-4 88 2.0438332150343204E-4 5.839523471526629E-5 0.0 2.5879016168764566 1.4598808678816574E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATATA 2535 0.0 18.957302 1 TACACCG 300 8.1684266E-7 16.747717 5 TACCGTG 360 1.960143E-8 16.613241 7 GTATTAG 1320 0.0 16.075657 1 GTACTGG 1385 0.0 15.771828 1 GTCTCGC 480 4.242611E-8 15.602844 1 TAGCGCT 290 7.088837E-5 15.572818 4 GTACATG 14240 0.0 15.515189 1 TAATACT 1915 0.0 15.328857 4 TCTATAC 1240 0.0 15.023967 3 TACATGG 14515 0.0 15.002985 2 TACACTG 1970 0.0 14.792399 5 GTATAGG 935 0.0 14.685029 1 CTGTGCG 560 1.0004442E-10 14.363491 9 CATGGGG 3930 0.0 14.220598 4 GTCCTAA 1055 0.0 14.197848 1 GTCTAGG 975 0.0 14.082568 1 AGTACCG 250 9.819192E-4 14.068081 5 ACATGGG 14845 0.0 13.956553 3 GTATAGA 1175 0.0 13.810177 1 >>END_MODULE