FastQCFastQC Report
Wed 25 May 2016
SRR1295089_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295089_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3424937
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT98370.2872169619470373No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA71020.20736147847391062No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGTACT40950.018.11387612-13
TACACCG2102.134142E-517.9145455
ACTCGCG2957.2561306E-817.5060628
TCTACGC2355.6019468E-516.008043
CTCGCGT3454.3074397E-714.9687329
GTACATG179350.013.8500111
GTACTTT55000.013.78515814-15
CTACGCT2400.00118934113.7158244
GTCCTAG12200.013.4919971
TACATGG179500.013.4659082
GTGTCGC2100.00788292113.4135399
ACATGGG173450.013.3927153
AGTACTT51700.013.21236912-13
ACACCGT3205.2570867E-513.2060746
GTATCAA152250.013.1373931
GTGTAAG16800.013.063681
ATACACC16950.013.039574
CATGGGT25750.012.9663054
TATACTG20050.012.6652725
GTATATA29700.012.5958351
Produced by FastQC (version 0.11.5)