##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1295089_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3424937 Sequences flagged as poor quality 0 Sequence length 100 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.12273218456281 33.0 33.0 33.0 27.0 33.0 2 29.7535364884084 33.0 33.0 33.0 22.0 33.0 3 30.610118667876225 33.0 33.0 33.0 27.0 33.0 4 34.902140973687985 37.0 37.0 37.0 33.0 37.0 5 35.212172077909756 37.0 37.0 37.0 33.0 37.0 6 34.873782203877035 37.0 37.0 37.0 33.0 37.0 7 34.77464957749588 37.0 37.0 37.0 27.0 37.0 8 34.75760138069693 37.0 37.0 37.0 27.0 37.0 9 34.74471034065736 37.0 37.0 37.0 27.0 37.0 10-11 34.70937480017881 37.0 37.0 37.0 27.0 37.0 12-13 34.60703174978109 37.0 37.0 37.0 27.0 37.0 14-15 35.97512932354668 37.0 37.0 40.0 27.0 40.0 16-17 35.96750932936868 37.0 37.0 40.0 27.0 40.0 18-19 35.99979503272615 37.0 37.0 40.0 27.0 40.0 20-21 36.00279173018365 37.0 37.0 40.0 27.0 40.0 22-23 35.96551892779341 37.0 37.0 40.0 27.0 40.0 24-25 35.93163626075457 37.0 37.0 40.0 27.0 40.0 26-27 35.9040634908029 37.0 37.0 40.0 27.0 40.0 28-29 35.84738274017887 37.0 37.0 40.0 27.0 40.0 30-31 35.75828504290736 37.0 37.0 40.0 27.0 40.0 32-33 35.71301720294417 37.0 37.0 40.0 27.0 40.0 34-35 35.59565971578455 37.0 37.0 40.0 27.0 40.0 36-37 35.595048463665165 37.0 37.0 40.0 27.0 40.0 38-39 35.5345637014637 37.0 37.0 40.0 27.0 40.0 40-41 35.48047117363035 37.0 37.0 40.0 27.0 40.0 42-43 35.391966042003105 37.0 37.0 40.0 27.0 40.0 44-45 35.280857282922284 37.0 33.0 40.0 27.0 40.0 46-47 35.185862688861135 37.0 33.0 40.0 27.0 40.0 48-49 35.09607621979616 37.0 33.0 40.0 27.0 40.0 50-51 35.010669685311 37.0 33.0 40.0 24.5 40.0 52-53 34.92222367885891 37.0 33.0 40.0 22.0 40.0 54-55 34.806680677629984 37.0 33.0 40.0 22.0 40.0 56-57 34.678399923852616 37.0 33.0 40.0 22.0 40.0 58-59 34.56118813280361 37.0 33.0 40.0 22.0 40.0 60-61 34.406788212454714 37.0 33.0 40.0 22.0 40.0 62-63 34.22075486352011 37.0 33.0 40.0 22.0 40.0 64-65 34.058056250377746 37.0 33.0 40.0 22.0 40.0 66-67 33.84391523114148 37.0 33.0 40.0 22.0 40.0 68-69 33.633340992841624 37.0 33.0 37.0 22.0 40.0 70-71 33.4048944549929 37.0 33.0 37.0 22.0 40.0 72-73 33.19053562153114 37.0 33.0 37.0 22.0 40.0 74-75 32.96050569689311 37.0 33.0 37.0 22.0 40.0 76-77 32.717563417954835 37.0 33.0 37.0 22.0 38.5 78-79 32.483259546088 37.0 33.0 37.0 22.0 37.0 80-81 32.26519246923374 37.0 33.0 37.0 22.0 37.0 82-83 32.06220012222123 35.0 33.0 37.0 22.0 37.0 84-85 31.88563161891737 33.0 33.0 37.0 22.0 37.0 86-87 31.885038615308837 33.0 33.0 37.0 22.0 37.0 88-89 31.89610349037077 33.0 33.0 37.0 22.0 37.0 90-91 31.890776093107696 33.0 33.0 37.0 22.0 37.0 92-93 31.848184360763426 33.0 33.0 37.0 22.0 37.0 94-95 31.867054634873575 33.0 33.0 37.0 22.0 37.0 96-97 31.817039408316123 33.0 33.0 37.0 22.0 37.0 98-99 31.737076331623037 33.0 33.0 37.0 22.0 37.0 100 31.19804130703718 33.0 27.0 37.0 15.0 37.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.6097887574772081 1101 2 4.110685323356073 1101 3 2.3442016278022173 1101 4 1.1407907112656872 1101 5 0.536490895599492 1101 6 0.5803184709782414 1101 7 0.5784139853884653 1101 8 0.5957329847348092 1101 9 0.6642463852540459 1101 10-11 0.5953062515537937 1101 12-13 0.7082013047707534 1101 14-15 1.051371725324266 1101 16-17 1.0369407137095692 1101 18-19 1.0703849494836462 1101 20-21 1.067213534710291 1101 22-23 1.0375636305135387 1101 24-25 0.9643609349174866 1101 26-27 1.0210779160182426 1101 28-29 0.837259906627672 1101 30-31 0.8353148003536006 1101 32-33 1.0862294922531888 1101 34-35 1.203057273843136 1101 36-37 0.9923365685648946 1101 38-39 1.0021421631876706 1101 40-41 1.0248258089847653 1101 42-43 1.0671483260287857 1101 44-45 1.0928678085674832 1101 46-47 1.1134682023641815 1101 48-49 1.072127598380817 1101 50-51 1.1009756939662552 1101 52-53 0.9660171038496728 1101 54-55 0.9161376568857875 1101 56-57 0.9305381594429178 1101 58-59 0.9411519599978817 1101 60-61 1.0400243018650457 1101 62-63 0.9779806173364705 1101 64-65 0.9898344091498572 1101 66-67 0.9726449049228378 1101 68-69 0.9280386501455737 1101 70-71 0.9858708851894633 1101 72-73 0.9938570309973258 1101 74-75 0.89571564736287 1101 76-77 0.936050284544514 1101 78-79 1.0069613743180454 1101 80-81 1.0200624791558965 1101 82-83 0.9072079897461727 1101 84-85 0.8736257327325845 1101 86-87 0.9074940417011206 1101 88-89 0.8538313897293186 1101 90-91 0.9487545421730417 1101 92-93 0.9099715831863762 1101 94-95 0.9440497693664085 1101 96-97 0.8756946387074223 1101 98-99 0.8626729626598895 1101 100 0.9767800356723804 1102 1 0.5028955188903836 1102 2 3.9861234713333893 1102 3 2.260397206451362 1102 4 1.0835816892023686 1102 5 0.48744073115516784 1102 6 0.521710749789932 1102 7 0.5614641383113508 1102 8 0.4910065785798423 1102 9 0.5882526428502999 1102 10-11 0.5752014780364973 1102 12-13 0.5898465634640999 1102 14-15 0.8960205485610828 1102 16-17 0.9021010564571341 1102 18-19 0.8725403238617631 1102 20-21 0.9068552108322763 1102 22-23 0.8481554770214075 1102 24-25 0.8959691525530928 1102 26-27 0.871989742006761 1102 28-29 0.860786007266853 1102 30-31 0.8819408015560413 1102 32-33 1.0536525545263586 1102 34-35 1.1469295986108392 1102 36-37 0.9325555900266025 1102 38-39 0.9470835023709157 1102 40-41 0.9337280083065451 1102 42-43 0.9759577665702039 1102 44-45 1.0375447605245895 1102 46-47 1.0075376845166417 1102 48-49 0.9479767989093801 1102 50-51 0.9365818085102191 1102 52-53 0.978649076774964 1102 54-55 0.9334790371808097 1102 56-57 0.8686625905135443 1102 58-59 0.9006378218762876 1102 60-61 0.873578472125871 1102 62-63 0.9162063457305862 1102 64-65 0.8341484767520413 1102 66-67 0.9509363581003001 1102 68-69 0.939676190406054 1102 70-71 0.9055478497306666 1102 72-73 0.9438256611728377 1102 74-75 0.8707787482220581 1102 76-77 0.8832164548664139 1102 78-79 0.849835709316082 1102 80-81 0.861558715662504 1102 82-83 0.8867235773134361 1102 84-85 0.9098217077341886 1102 86-87 0.8883754885598663 1102 88-89 0.8092029508660197 1102 90-91 0.8852173317333651 1102 92-93 0.9252255506866263 1102 94-95 0.8204521668178089 1102 96-97 0.8470851592876656 1102 98-99 0.9274777552509441 1102 100 0.8392336882085232 1103 1 0.4807274540927615 1103 2 3.987363277270351 1103 3 2.2255136408630953 1103 4 1.0079080409913388 1103 5 0.47117030852356834 1103 6 0.5032696210038949 1103 7 0.5036882545925536 1103 8 0.49573839088884597 1103 9 0.5638143640841236 1103 10-11 0.608239306513326 1103 12-13 0.6273933527222795 1103 14-15 0.8567622475112557 1103 16-17 0.7820777496581499 1103 18-19 0.8673815003648215 1103 20-21 0.8189908567339899 1103 22-23 0.816816375626523 1103 24-25 0.8066528812768894 1103 26-27 0.7869024020895523 1103 28-29 0.7992955577087884 1103 30-31 0.7345540894877374 1103 32-33 0.8805723068161555 1103 34-35 0.9800246106351551 1103 36-37 0.9132037979843233 1103 38-39 0.8078404525281968 1103 40-41 0.80370401544819 1103 42-43 0.8156244302785822 1103 44-45 0.8835958651828619 1103 46-47 0.8940425500832063 1103 48-49 0.8743981849381015 1103 50-51 0.7921697413195119 1103 52-53 0.7642985614136037 1103 54-55 0.8050631196161646 1103 56-57 0.7697363629237444 1103 58-59 0.8457984832812286 1103 60-61 0.8192446901311925 1103 62-63 0.8958375333298818 1103 64-65 0.8146742548627302 1103 66-67 0.8818443764196076 1103 68-69 0.8399370644328812 1103 70-71 0.878284592924139 1103 72-73 0.821558991122032 1103 74-75 0.8002610217463015 1103 76-77 0.7255000082710481 1103 78-79 0.6698358340541404 1103 80-81 0.7650108397699427 1103 82-83 0.8491203544435741 1103 84-85 0.8836206370180548 1103 86-87 0.8376615600341353 1103 88-89 0.8204260134497297 1103 90-91 0.8447144858306004 1103 92-93 0.812180076256233 1103 94-95 0.6748175346766843 1103 96-97 0.6614743604080005 1103 98-99 0.789783275745112 1103 100 0.7388920321516572 1104 1 0.35098098582711756 1104 2 3.5124101654634927 1104 3 2.0705906773859155 1104 4 1.04308724327133 1104 5 0.3949775399124533 1104 6 0.423746574072986 1104 7 0.360555850573526 1104 8 0.3411579737924484 1104 9 0.37449018180787874 1104 10-11 0.44298385305243926 1104 12-13 0.38960479361968225 1104 14-15 0.5658907197760215 1104 16-17 0.5146242889895518 1104 18-19 0.455694962202422 1104 20-21 0.5508338882592199 1104 22-23 0.5226136497675213 1104 24-25 0.4639249198086688 1104 26-27 0.5241440855728285 1104 28-29 0.5276038584029905 1104 30-31 0.4558138659135409 1104 32-33 0.5634733746346186 1104 34-35 0.7167442152764707 1104 36-37 0.5401135623313067 1104 38-39 0.46967304039043256 1104 40-41 0.47862898883930427 1104 42-43 0.5262666438648012 1104 44-45 0.522447207525822 1104 46-47 0.5838699978911635 1104 48-49 0.3967738391036377 1104 50-51 0.39186448777468996 1104 52-53 0.31583878171064583 1104 54-55 0.4145598757045974 1104 56-57 0.39086579459146975 1104 58-59 0.43777193049375285 1104 60-61 0.4456224664077766 1104 62-63 0.46831287148869905 1104 64-65 0.5243620058767604 1104 66-67 0.32556896730881846 1104 68-69 0.4280804099469435 1104 70-71 0.45811089069263744 1104 72-73 0.40834188017360873 1104 74-75 0.4174882845996919 1104 76-77 0.3691418319741686 1104 78-79 0.3772738014307322 1104 80-81 0.3744865731220699 1104 82-83 0.42948561941368624 1104 84-85 0.36348740805813406 1104 86-87 0.3627193815673557 1104 88-89 0.3748248327931343 1104 90-91 0.34890592825506417 1104 92-93 0.41533229949072137 1104 94-95 0.44745571227683456 1104 96-97 0.3400455600444161 1104 98-99 0.3440572388102616 1104 100 0.3261763471269816 1105 1 0.5057217055115508 1105 2 3.7411006054641263 1105 3 2.1776588995606936 1105 4 1.0872271305751653 1105 5 0.4317343941871741 1105 6 0.4252155288291988 1105 7 0.35915131652750176 1105 8 0.32007272375710727 1105 9 0.45526721201389364 1105 10-11 0.43063029344337167 1105 12-13 0.4348779032168153 1105 14-15 0.6169811631640343 1105 16-17 0.576393476389967 1105 18-19 0.5797178627819477 1105 20-21 0.53398865701174 1105 22-23 0.5048322389154052 1105 24-25 0.5570377693548281 1105 26-27 0.5737118146683216 1105 28-29 0.5146468650112936 1105 30-31 0.4924690420779072 1105 32-33 0.6076895157955136 1105 34-35 0.672667075785867 1105 36-37 0.543287047870912 1105 38-39 0.5310066035312957 1105 40-41 0.5626792546106429 1105 42-43 0.5911391212921373 1105 44-45 0.6336702914170473 1105 46-47 0.5898480715614554 1105 48-49 0.5440033175820744 1105 50-51 0.5048194358844498 1105 52-53 0.39775154828110004 1105 54-55 0.4565072755898427 1105 56-57 0.4026030948745003 1105 58-59 0.4416749125261603 1105 60-61 0.44172345396139434 1105 62-63 0.6017836608741476 1105 64-65 0.4591600433587999 1105 66-67 0.3998703362375622 1105 68-69 0.3862789585075106 1105 70-71 0.46661888549557773 1105 72-73 0.43831396528864275 1105 74-75 0.4406522839628835 1105 76-77 0.4205635467665232 1105 78-79 0.4573734690402773 1105 80-81 0.43909873076937345 1105 82-83 0.4816422747025122 1105 84-85 0.5201159210803254 1105 86-87 0.5847907353674415 1105 88-89 0.464914644109637 1105 90-91 0.4483376687850793 1105 92-93 0.47220300829734896 1105 94-95 0.4758434789049488 1105 96-97 0.5112218402481901 1105 98-99 0.501849388715744 1105 100 0.4544994718822615 1106 1 0.5313959689273098 1106 2 3.8760207570782725 1106 3 2.209188155861451 1106 4 1.0043874380816717 1106 5 0.4647367305433363 1106 6 0.3348708448516433 1106 7 0.33722624431400305 1106 8 0.29058744166530204 1106 9 0.27061548676952896 1106 10-11 0.33497028050633304 1106 12-13 0.36607915369189925 1106 14-15 0.41927037145866564 1106 16-17 0.3563830079802983 1106 18-19 0.4442460671220303 1106 20-21 0.3777134523327348 1106 22-23 0.27089942793516997 1106 24-25 0.3416178372831311 1106 26-27 0.33957136688735545 1106 28-29 0.2431371175963477 1106 30-31 0.27718737008876104 1106 32-33 0.37813786365129687 1106 34-35 0.5237078615328201 1106 36-37 0.4839590348776497 1106 38-39 0.380706276716964 1106 40-41 0.4032366439013515 1106 42-43 0.4032065744889124 1106 44-45 0.3863442179559158 1106 46-47 0.30779251377701655 1106 48-49 0.2822836555610948 1106 50-51 0.32079467957564844 1106 52-53 0.2852239914446457 1106 54-55 0.3619761120576612 1106 56-57 0.29805861730502414 1106 58-59 0.3512448317939274 1106 60-61 0.4228212909585878 1106 62-63 0.4767582601335363 1106 64-65 0.3240625915499251 1106 66-67 0.2995556044223733 1106 68-69 0.2335512191673743 1106 70-71 0.2519365453191824 1106 72-73 0.24734995470555532 1106 74-75 0.26737103327928935 1106 76-77 0.30190107925457 1106 78-79 0.3288482721106476 1106 80-81 0.2737575258671541 1106 82-83 0.32039013524572013 1106 84-85 0.2728800010494403 1106 86-87 0.3242869112702387 1106 88-89 0.4061994417279884 1106 90-91 0.3844274133832499 1106 92-93 0.39950632604598013 1106 94-95 0.4490094616559972 1106 96-97 0.3958480041792427 1106 98-99 0.41430682414899067 1106 100 0.355486561184982 1107 1 0.4462031808007261 1107 2 3.552260962511877 1107 3 2.097128294261818 1107 4 0.9903204897398084 1107 5 0.32144664418963487 1107 6 0.1795761712213988 1107 7 0.14015556980842803 1107 8 0.12519147140588416 1107 9 0.10854413097146676 1107 10-11 0.1500242902353932 1107 12-13 0.16626507641502997 1107 14-15 0.12089126242206305 1107 16-17 0.09297303086745501 1107 18-19 0.14391213627613553 1107 20-21 0.13408134507510283 1107 22-23 0.05253748542585868 1107 24-25 0.1103015569747825 1107 26-27 0.0831086226263551 1107 28-29 -0.038548597859680456 1107 30-31 0.059617092237530755 1107 32-33 0.13552026453344723 1107 34-35 0.23678868659985142 1107 36-37 0.1057946031011241 1107 38-39 -0.042704400729263625 1107 40-41 0.02869740335810178 1107 42-43 0.09056847343637031 1107 44-45 0.2853546951848145 1107 46-47 0.12432084602409788 1107 48-49 -0.015918289563792598 1107 50-51 0.096942383415616 1107 52-53 0.026758581644202195 1107 54-55 0.0744748430517177 1107 56-57 0.041750373647339245 1107 58-59 -0.024432942773216837 1107 60-61 0.02165684274188351 1107 62-63 0.10459362345841328 1107 64-65 0.05659551999025325 1107 66-67 0.018999023290191985 1107 68-69 0.0033968978271090577 1107 70-71 0.001422965957061706 1107 72-73 0.10220872189030672 1107 74-75 0.061183680724319345 1107 76-77 0.08760026228874551 1107 78-79 0.0958973078008114 1107 80-81 0.1337070572800343 1107 82-83 0.18826002853590396 1107 84-85 0.09369704861294537 1107 86-87 0.09598594699290786 1107 88-89 0.08513819701636649 1107 90-91 0.04787915971655465 1107 92-93 0.1601920155878176 1107 94-95 0.053610168992712204 1107 96-97 0.021869894148693447 1107 98-99 0.03220822779728039 1107 100 0.0740076019769873 1108 1 0.430239687188017 1108 2 3.5984778788129574 1108 3 2.030933889304986 1108 4 0.9173925893472159 1108 5 0.22785089904154177 1108 6 0.12342419832188511 1108 7 -0.004311010834726403 1108 8 0.024708304230237843 1108 9 0.08510448809757776 1108 10-11 0.11400237056631113 1108 12-13 0.04502421799870859 1108 14-15 -0.08867998821023093 1108 16-17 -0.008523794224004178 1108 18-19 0.13325266382118883 1108 20-21 0.0587391014258003 1108 22-23 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0.19134587522155755 2211 18-19 0.19809156882687518 2211 20-21 0.22385101167550658 2211 22-23 0.14717842334994913 2211 24-25 0.15847920157276718 2211 26-27 0.12300921840615331 2211 28-29 0.02725237671634062 2211 30-31 -0.06307887746842056 2211 32-33 0.07717199221242055 2211 34-35 0.2566129721301351 2211 36-37 0.09754057988066478 2211 38-39 0.03194333024890739 2211 40-41 0.012089512294721771 2211 42-43 0.1609767796241357 2211 44-45 0.029946168293186304 2211 46-47 0.04536536757580478 2211 48-49 0.10560392767457927 2211 50-51 0.0588730264944175 2211 52-53 0.12163942819789497 2211 54-55 0.011464490481550627 2211 56-57 0.010894413642297707 2211 58-59 0.09683790331091302 2211 60-61 0.10638330014909769 2211 62-63 0.21920801055571815 2211 64-65 0.16400621309794872 2211 66-67 0.0952119921715493 2211 68-69 0.1537866789662914 2211 70-71 -0.04405816999521761 2211 72-73 0.022480880433512596 2211 74-75 0.07606932746157469 2211 76-77 -0.03770207436701156 2211 78-79 -0.015575693982015082 2211 80-81 0.06630786490538298 2211 82-83 -0.1850170358672969 2211 84-85 -0.043029855631637304 2211 86-87 0.059149816954384704 2211 88-89 0.13545144066650394 2211 90-91 0.1507243493725703 2211 92-93 0.20350381847904941 2211 94-95 0.23625836008064027 2211 96-97 0.03859009784378742 2211 98-99 0.16635475686940993 2211 100 0.0819554551315882 2212 1 0.5318360253233081 2212 2 4.07357538866232 2212 3 2.3990709398997083 2212 4 1.2061723682042853 2212 5 0.7330958757663026 2212 6 0.5471751742948285 2212 7 0.49195485080933565 2212 8 0.5315956153850081 2212 9 0.3581968852314219 2212 10-11 0.4229460265262617 2212 12-13 0.3789514970697496 2212 14-15 0.4855639941507093 2212 16-17 0.5382717243727342 2212 18-19 0.4326988719305902 2212 20-21 0.5803124895087137 2212 22-23 0.5353239128537055 2212 24-25 0.518031206247592 2212 26-27 0.5060597418783104 2212 28-29 0.4225803295346253 2212 30-31 0.43465042619255456 2212 32-33 0.5677423951384171 2212 34-35 0.5309082006488595 2212 36-37 0.5911216914100166 2212 38-39 0.5693676280987603 2212 40-41 0.4429856207579874 2212 42-43 0.4961636043721498 2212 44-45 0.6143826721483165 2212 46-47 0.4973648586240884 2212 48-49 0.6035062690292037 2212 50-51 0.5834576329433787 2212 52-53 0.5497388057334476 2212 54-55 0.4195349390600498 2212 56-57 0.6203448046927562 2212 58-59 0.5704372110423321 2212 60-61 0.6497365320808726 2212 62-63 0.5943328185943244 2212 64-65 0.6454042928655284 2212 66-67 0.6520824869327058 2212 68-69 0.5181024481637593 2212 70-71 0.6514440870053733 2212 72-73 0.643725815215241 2212 74-75 0.6755710083570889 2212 76-77 0.6404037828779181 2212 78-79 0.60714160749 2212 80-81 0.5875707367007976 2212 82-83 0.666204566070018 2212 84-85 0.7101829020914394 2212 86-87 0.5094351303396323 2212 88-89 0.5561487810992425 2212 90-91 0.5784847202947034 2212 92-93 0.7212320088081441 2212 94-95 0.7939660627468044 2212 96-97 0.4970843194156842 2212 98-99 0.5812565863269974 2212 100 0.43013056279784934 2213 1 -0.7481994945154398 2213 2 -25.63425834435127 2213 3 -14.588856582558712 2213 4 -6.312894103464373 2213 5 -2.5430569186306258 2213 6 -1.5870164457151148 2213 7 -1.542969160700146 2213 8 -1.1839461196048262 2213 9 -1.350130908385836 2213 10-11 -1.2439377149405217 2213 12-13 -1.2978282290466865 2213 14-15 -1.4627352719805344 2213 16-17 -1.5633061509040402 2213 18-19 -1.5878030017244882 2213 20-21 -1.709343260224145 2213 22-23 -1.676593616777616 2213 24-25 -1.5526042771521347 2213 26-27 -1.6326789591526563 2213 28-29 -1.6400164103219481 2213 30-31 -1.499291947629132 2213 32-33 -1.5231093587767148 2213 34-35 -1.4877331491050114 2213 36-37 -1.526433545714454 2213 38-39 -1.6006684169871406 2213 40-41 -1.508159007687098 2213 42-43 -1.5759257467342351 2213 44-45 -1.4117229320746745 2213 46-47 -1.4884990170105254 2213 48-49 -1.7793939816121735 2213 50-51 -1.6096343000810478 2213 52-53 -1.7411254228015878 2213 54-55 -1.5331973309760798 2213 56-57 -1.5366720869274175 2213 58-59 -1.6334719408968965 2213 60-61 -1.5983061015740105 2213 62-63 -1.5708442799732296 2213 64-65 -1.6315384240082054 2213 66-67 -1.6029081817233362 2213 68-69 -1.5497824636193371 2213 70-71 -1.547794468761925 2213 72-73 -1.722478962235904 2213 74-75 -1.6525287295117508 2213 76-77 -1.6915659596032455 2213 78-79 -1.63176008429825 2213 80-81 -1.7298119074918503 2213 82-83 -1.7024988285038525 2213 84-85 -1.5247010593225916 2213 86-87 -1.4616192376649302 2213 88-89 -1.6069962675435683 2213 90-91 -1.413643137993617 2213 92-93 -1.4970147490217336 2213 94-95 -1.6508472857530379 2213 96-97 -1.763832204039062 2213 98-99 -1.6176577934628078 2213 100 -1.5834454347203568 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3.0 3 20.0 4 26.0 5 60.0 6 54.0 7 50.0 8 58.0 9 120.0 10 570.0 11 2842.0 12 8451.0 13 15554.0 14 20586.0 15 23059.0 16 23995.0 17 24530.0 18 25019.0 19 25748.0 20 27855.0 21 30538.0 22 33032.0 23 35093.0 24 36377.0 25 38012.0 26 41926.0 27 47212.0 28 54276.0 29 64689.0 30 77185.0 31 93642.0 32 117232.0 33 153727.0 34 207685.0 35 288561.0 36 428894.0 37 718497.0 38 752376.0 39 7383.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 37.96063902891327 21.137945195163393 10.124233395932468 30.77718237999087 2 12.343934907580252 23.2256609014701 36.49280299466724 27.937601196282415 3 14.675773598171297 28.04142090788823 29.00789124004325 28.274914253897226 4 9.990370122502085 18.951295244802697 38.22678857893025 32.83154605376497 5 11.291032042999307 38.66079904820342 35.562926961661994 14.48524194713528 6 29.248033822833857 40.29946716533066 17.893767858900915 12.558731152934563 7 25.094496742231932 33.56080899317486 24.08931603373346 17.255378230859755 8 23.80761263140184 37.521075120316596 21.40943043720578 17.261881811075785 9 24.29109223118055 16.724171069073634 22.549047672181675 36.43568902756414 10-11 22.56346525491259 28.089911107164472 29.390515059670836 19.956108578252103 12-13 23.234059743502414 25.84292341584728 29.90744565068167 21.015571189968636 14-15 21.05558800371384 26.86868164617759 26.951566851701497 25.12416349840707 16-17 20.250630490090398 30.76233769325257 27.926596334898928 21.060435481758102 18-19 20.177085669825605 29.66707029570646 29.98388404456158 20.171959989906355 20-21 21.535398652004215 29.033572270602043 28.869617760239453 20.56141131715429 22-23 20.379579242456522 29.245223645631807 29.291936193492457 21.083260918419214 24-25 20.803523711900194 28.62722422267196 29.060577122447967 21.508674942979884 26-27 20.809973441339526 28.836251768508937 29.014832843966776 21.338941946184768 28-29 20.28230193460362 28.741372359396966 29.68118632987773 21.295139376121693 30-31 21.29982412866002 29.06656476136461 28.720925885796362 20.912685224179008 32-33 20.0298492122792 29.252972585032882 29.613131083970508 21.10404711871741 34-35 20.25458050890699 28.470363542742 29.751164334493836 21.523891613857177 36-37 21.005957027585872 29.16165235211562 28.88691925623803 20.945471364060477 38-39 20.612997351894887 29.052536665116175 29.278190610424875 21.056275372564066 40-41 20.973598333054962 28.79449868426739 28.892580038940547 21.339322943737102 42-43 20.484527182603905 29.077967278588968 29.322236885309206 21.11526865349792 44-45 21.052336504269345 28.266644202057673 29.185381496364883 21.495637797308103 46-47 20.940094938141275 29.166626512884985 28.98910299371995 20.904175555253794 48-49 20.711790417882405 28.89990065751785 29.250731634089643 21.137577290510098 50-51 21.160538981980682 28.79578606016835 28.603111012076248 21.440563945774716 52-53 20.365659011289576 29.236745225915644 28.833980002657743 21.563615760137033 54-55 20.96108460431142 28.765468803344792 28.553630198456865 21.719816393886923 56-57 21.17316607748364 28.765530756054865 28.511586568583546 21.54971659787795 58-59 20.4851219930283 28.443191936028818 29.306127145146167 21.765558925796714 60-61 21.351311179902766 28.81126677648283 28.36769979713892 21.46972224647548 62-63 20.31149454742177 29.252671533358303 28.93151076972722 21.504323149492706 64-65 20.490531974480923 28.352853841207242 29.08730406727055 22.069310117041276 66-67 20.9114522834785 28.819590107406363 28.625853602195058 21.643104006920076 68-69 20.97059397767303 28.659565467101032 28.795795997956176 21.574044557269758 70-71 21.188714636495263 28.61286933309579 28.314767044612392 21.88364898579655 72-73 20.67321803172259 28.955328973479617 28.906133866180543 21.465319128617242 74-75 21.25566885932409 28.102384889135894 28.602417087570544 22.03952916396947 76-77 20.97480460585116 28.835353480634634 28.654995266911026 21.534846646603178 78-79 20.86737252777658 28.70476700815977 28.90369106031747 21.52416940374618 80-81 21.179456031023868 28.515035947273436 28.45580033056307 21.849707691139624 82-83 20.38781300132626 28.916630118090346 28.837410713920715 21.858146166662674 84-85 20.74150915297395 28.231179388945183 28.597969964112284 22.429341493968586 86-87 21.018071675250045 28.70636659433792 28.38934066373672 21.88622106667531 88-89 20.529023373808066 28.26912991782025 28.96858049550791 22.23326621286377 90-91 21.101768780979217 28.291093315424092 28.462584040821394 22.1445538627753 92-93 20.267154481396346 28.8494283238644 28.810404118783666 22.073013075955586 94-95 20.3470822746631 28.021144596007936 28.998649301263047 22.63312382806592 96-97 20.75229907199143 28.444403502627587 28.615415070945566 22.187882354435416 98-99 20.556062785718435 28.186652005492686 28.818447969134454 22.438837239654426 100 20.808026659659376 28.012869172474332 28.544232592140943 22.634871575725352 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 23.0 1 23.0 2 24.5 3 29.5 4 34.0 5 38.0 6 66.0 7 112.5 8 180.5 9 217.0 10 201.0 11 200.0 12 229.0 13 260.5 14 346.0 15 458.5 16 543.0 17 708.0 18 926.0 19 1256.5 20 1762.5 21 2532.5 22 3706.0 23 5445.5 24 8106.0 25 11956.5 26 17665.5 27 25650.5 28 35813.0 29 47716.0 30 60555.0 31 73648.5 32 86646.0 33 98947.5 34 110621.5 35 121319.5 36 130001.5 37 137664.0 38 144385.0 39 149198.5 40 151389.0 41 150533.0 42 146782.0 43 141506.5 44 134957.0 45 126518.0 46 118439.0 47 111797.0 48 105858.0 49 103394.0 50 113196.5 51 107970.0 52 85372.0 53 74652.0 54 68586.5 55 62144.0 56 56259.0 57 50503.5 58 44477.5 59 39075.0 60 33294.0 61 27070.5 62 22092.0 63 17514.0 64 13413.5 65 10383.5 66 7826.0 67 5858.5 68 4330.0 69 3096.0 70 2231.0 71 1608.0 72 1172.5 73 836.5 74 602.5 75 420.0 76 248.5 77 147.5 78 84.5 79 34.5 80 21.0 81 16.0 82 6.0 83 1.0 84 1.5 85 4.5 86 3.5 87 0.5 88 0.0 89 0.5 90 0.5 91 0.0 92 1.5 93 1.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content warn #Base N-Count 1 0.7453859735230166 2 5.652454337116274 3 0.0 4 0.005226373507016333 5 0.16698117366830398 6 0.05617621579608618 7 0.03719776451362463 8 0.04289129989836309 9 0.04178179043877304 10-11 0.04347525224551576 12-13 0.041898580908203566 14-15 0.04459936051378464 16-17 0.03537291342877256 18-19 0.029226814974990783 20-21 0.023839854572507466 22-23 0.023460285546858237 24-25 0.020642715471846637 26-27 0.01290534687207385 28-29 0.020306942872233853 30-31 0.024613591432484743 32-33 0.0016350665720274561 34-35 0.003693498595740593 36-37 0.004627822351184854 38-39 0.0065402662881098245 40-41 0.006686254374897991 42-43 0.006934434122437873 44-45 0.008496506651071246 46-47 0.017576965649295156 48-49 0.026963999629774214 50-51 0.02271574630423859 52-53 0.028672060245195752 54-55 0.025343531866425573 56-57 0.016423659763668646 58-59 0.015182761025969235 60-61 0.012438184994351722 62-63 0.0186718763002064 64-65 0.019708391716402374 66-67 0.020394535724306755 68-69 0.02807350908936427 70-71 0.027577149594284506 72-73 0.02382525576382865 74-75 0.025036956884170423 76-77 0.01870107391756403 78-79 0.015022174130502254 80-81 0.013927263479591011 82-83 0.007912554303918583 84-85 0.010715525570251365 86-87 0.008642494737859413 88-89 0.007270206722050654 90-91 0.007649775747699885 92-93 0.004073067621389825 94-95 0.0012554975463782254 96-97 6.569463905467458E-4 98-99 5.255571124373967E-4 100 0.1091406936828327 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 3424937.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 85.50912887804692 #Duplication Level Percentage of deduplicated Percentage of total 1 90.65635922602434 77.51946304672632 2 7.262354453712798 12.419952058811713 3 1.2090500293619928 3.1015444434216315 4 0.39360506578969695 1.3462730519065336 5 0.1488172905103859 0.6362618436767168 6 0.08663740075865346 0.4444973200278419 7 0.05350244975123499 0.32024635097487075 8 0.036143600493595295 0.24724862341787843 9 0.026052027558504283 0.20049175638311156 >10 0.12022116394163287 1.728865844752775 >50 0.004389674594299101 0.24843896743863325 >100 0.001840951043205917 0.29795556088294317 >500 5.817776635677958E-4 0.36347719199956713 >1k 3.7644436591234565E-4 0.6295959837741737 >5k 6.844443016588093E-5 0.49568795580528574 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 9837 0.2872169619470373 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 7102 0.20736147847391062 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 5.839523471526629E-5 0.0 5 0.0 0.0 0.0 5.839523471526629E-5 0.0 6 0.0 0.0 0.0 5.839523471526629E-5 0.0 7 0.0 0.0 0.0 8.759285207289944E-5 0.0 8 0.0 0.0 0.0 1.1679046943053258E-4 0.0 9 0.0 0.0 0.0 1.4598808678816574E-4 0.0 10-11 0.0 0.0 0.0 1.751857041457989E-4 0.0 12-13 2.9197617357633146E-5 0.0 0.0 2.0438332150343204E-4 0.0 14-15 2.9197617357633146E-5 0.0 0.0 2.0438332150343204E-4 0.0 16-17 2.9197617357633146E-5 0.0 0.0 2.3358093886106517E-4 0.0 18-19 2.9197617357633146E-5 0.0 0.0 3.357725996127812E-4 0.0 20-21 2.9197617357633146E-5 0.0 0.0 4.963594950797635E-4 0.0 22-23 2.9197617357633146E-5 0.0 0.0 5.401559211162133E-4 0.0 24-25 2.9197617357633146E-5 0.0 0.0 6.423475818679292E-4 0.0 26-27 2.9197617357633146E-5 0.0 0.0 7.737368599772784E-4 0.0 28-29 2.9197617357633146E-5 0.0 0.0 0.0015474737199545566 0.0 30-31 2.9197617357633146E-5 0.0 0.0 0.0043504449862873384 0.0 32-33 2.9197617357633146E-5 0.0 0.0 0.01179583741248379 0.0 34-35 5.839523471526629E-5 0.0 0.0 0.022642752260844508 0.0 36-37 5.839523471526629E-5 0.0 0.0 0.04004453220599386 0.0 38-39 5.839523471526629E-5 0.0 0.0 0.0677384722697089 0.0 40-41 5.839523471526629E-5 0.0 0.0 0.10325737378526963 0.0 42-43 5.839523471526629E-5 0.0 0.0 0.1370682146854088 0.0 44-45 5.839523471526629E-5 0.0 0.0 0.1778280885166647 0.0 46-47 5.839523471526629E-5 0.0 0.0 0.22739104398124696 0.0 48-49 5.839523471526629E-5 0.0 0.0 0.27730437085412085 0.0 50-51 5.839523471526629E-5 1.4598808678816573E-5 0.0 0.33442950921433007 0.0 52-53 7.299404339408287E-5 2.9197617357633146E-5 0.0 0.39546712830046216 0.0 54-55 8.759285207289944E-5 2.9197617357633146E-5 0.0 0.46235886966679973 0.0 56-57 8.759285207289944E-5 2.9197617357633146E-5 0.0 0.539776352090564 0.0 58-59 8.759285207289944E-5 2.9197617357633146E-5 0.0 0.6276903779543974 0.0 60-61 8.759285207289944E-5 2.9197617357633146E-5 0.0 0.7199986452305547 0.0 62-63 8.759285207289944E-5 2.9197617357633146E-5 0.0 0.8147303147473953 0.0 64-65 1.0219166075171602E-4 2.9197617357633146E-5 0.0 0.9232870560830755 0.0 66-67 1.1679046943053258E-4 2.9197617357633146E-5 0.0 1.0483258524171393 0.0 68-69 1.1679046943053258E-4 2.9197617357633146E-5 0.0 1.186926942013824 0.0 70-71 1.1679046943053258E-4 2.9197617357633146E-5 0.0 1.347046675603084 0.0 72-73 1.1679046943053258E-4 2.9197617357633146E-5 0.0 1.5077649603481758 0.0 74-75 1.1679046943053258E-4 2.9197617357633146E-5 0.0 1.6791257766201246 0.0 76-77 1.1679046943053258E-4 2.9197617357633146E-5 0.0 1.8711585059812779 0.0 78-79 1.1679046943053258E-4 2.9197617357633146E-5 0.0 2.0841551246052115 0.0 80-81 1.1679046943053258E-4 2.9197617357633146E-5 0.0 2.320772615671471 0.0 82-83 1.1679046943053258E-4 2.9197617357633146E-5 0.0 2.577419672245066 0.0 84-85 1.1679046943053258E-4 2.9197617357633146E-5 0.0 2.8431325890082064 0.0 86-87 1.1679046943053258E-4 2.9197617357633146E-5 0.0 3.13274375557857 0.0 88 1.1679046943053258E-4 2.9197617357633146E-5 0.0 3.37156566675533 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GAGTACT 4095 0.0 18.113876 12-13 TACACCG 210 2.134142E-5 17.914545 5 ACTCGCG 295 7.2561306E-8 17.506062 8 TCTACGC 235 5.6019468E-5 16.00804 3 CTCGCGT 345 4.3074397E-7 14.968732 9 GTACATG 17935 0.0 13.850011 1 GTACTTT 5500 0.0 13.785158 14-15 CTACGCT 240 0.001189341 13.715824 4 GTCCTAG 1220 0.0 13.491997 1 TACATGG 17950 0.0 13.465908 2 GTGTCGC 210 0.007882921 13.413539 9 ACATGGG 17345 0.0 13.392715 3 AGTACTT 5170 0.0 13.212369 12-13 ACACCGT 320 5.2570867E-5 13.206074 6 GTATCAA 15225 0.0 13.137393 1 GTGTAAG 1680 0.0 13.06368 1 ATACACC 1695 0.0 13.03957 4 CATGGGT 2575 0.0 12.966305 4 TATACTG 2005 0.0 12.665272 5 GTATATA 2970 0.0 12.595835 1 >>END_MODULE