FastQCFastQC Report
Wed 25 May 2016
SRR1295088_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295088_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2834702
Sequences flagged as poor quality0
Sequence length100
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAT16700.017.6923981
GTCCTAC7600.017.370451
CTAGTAC6550.017.3595263
GTATATA22250.016.1046071
CGTAGCA2350.00239568816.0466252
GTATTAG12550.016.0291881
GTATAGG7600.015.716121
CGTATGT2804.23762E-415.7161191
TATACTG14550.015.6227475
GTACATG104150.015.5124061
CCTATAC8850.015.4176113
TACATGG107100.014.9641162
GTGTAGG10600.014.2334661
GTAGGAC6401.1823431E-1014.2131113
ACATGGG106800.014.1066463
GTCTATA9200.013.6661911
CATGGGG34550.013.6570874
GTGTACA11550.013.607031
TATACAG16750.013.57080655
GTCTAGA9300.013.5192421
Produced by FastQC (version 0.11.5)