FastQCFastQC Report
Wed 25 May 2016
SRR1295088_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295088_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2834702
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT71780.25321885686749435No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA53170.18756821704715346No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTATAC9450.019.4091913
GGGTACG1750.001483345517.2397731
CTAGGAC11900.016.9940223
GTCTAGG9550.016.8486441
GTACTAC7950.016.4446471
GAGTACT33800.016.31714412-13
CTACGTA2053.6501623E-416.0278349
GTTAGAC6050.015.5470973
GTATCAA116300.015.4782431
CATGGGG37250.015.2771174
TAGGACC7400.014.617684
TACCGTT2257.3985907E-414.6041697
TACACCG3253.7404789E-614.4710155
CGTACCC1950.00476834414.4707593
CTATACT14050.014.3937674
TATCAAC125700.014.2278442
CAACGCA122650.014.2261925
TCTACAC13900.014.2104923
AACGCAG123050.014.1569166
CGTAGAG3002.8222632E-514.108993
Produced by FastQC (version 0.11.5)