##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1295088_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2834702 Sequences flagged as poor quality 0 Sequence length 100 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.205190880734552 33.0 33.0 33.0 27.0 33.0 2 29.675479821159332 33.0 33.0 33.0 22.0 33.0 3 30.62499726602655 33.0 33.0 33.0 22.0 33.0 4 34.997107279707 37.0 37.0 37.0 33.0 37.0 5 35.32999306452671 37.0 37.0 37.0 33.0 37.0 6 35.040080050742546 37.0 37.0 37.0 33.0 37.0 7 34.95440931709929 37.0 37.0 37.0 33.0 37.0 8 34.93991290795294 37.0 37.0 37.0 33.0 37.0 9 34.924981885221094 37.0 37.0 37.0 33.0 37.0 10-11 34.89401037569381 37.0 37.0 37.0 33.0 37.0 12-13 34.792153637313554 37.0 37.0 37.0 30.0 37.0 14-15 36.213789491805485 37.0 37.0 40.0 27.0 40.0 16-17 36.21275834285227 37.0 37.0 40.0 27.0 40.0 18-19 36.24325537569734 38.5 37.0 40.0 27.0 40.0 20-21 36.23979980964489 40.0 37.0 40.0 27.0 40.0 22-23 36.20271989789403 38.5 37.0 40.0 27.0 40.0 24-25 36.17220222795906 37.0 37.0 40.0 27.0 40.0 26-27 36.14565058337702 37.0 37.0 40.0 27.0 40.0 28-29 36.096532192801924 37.0 37.0 40.0 27.0 40.0 30-31 36.01427610380209 37.0 37.0 40.0 27.0 40.0 32-33 35.962275576057024 37.0 37.0 40.0 27.0 40.0 34-35 35.83995054859382 37.0 37.0 40.0 27.0 40.0 36-37 35.84945701523476 37.0 37.0 40.0 27.0 40.0 38-39 35.78944488697577 37.0 37.0 40.0 27.0 40.0 40-41 35.734656235470254 37.0 37.0 40.0 27.0 40.0 42-43 35.651079725487904 37.0 37.0 40.0 27.0 40.0 44-45 35.545121497780016 37.0 37.0 40.0 27.0 40.0 46-47 35.44235619828821 37.0 37.0 40.0 27.0 40.0 48-49 35.347048649205455 37.0 35.0 40.0 27.0 40.0 50-51 35.25606554057534 37.0 33.0 40.0 27.0 40.0 52-53 35.15643055248841 37.0 33.0 40.0 27.0 40.0 54-55 35.0337578694339 37.0 33.0 40.0 27.0 40.0 56-57 34.90870239623071 37.0 33.0 40.0 27.0 40.0 58-59 34.78199630860669 37.0 33.0 40.0 27.0 40.0 60-61 34.625454633326534 37.0 33.0 40.0 27.0 40.0 62-63 34.43559763954024 37.0 33.0 40.0 24.5 40.0 64-65 34.27450927822396 37.0 33.0 40.0 22.0 40.0 66-67 34.050229089336376 37.0 33.0 40.0 22.0 40.0 68-69 33.837651188731655 37.0 33.0 37.0 22.0 40.0 70-71 33.60551285461399 37.0 33.0 37.0 22.0 40.0 72-73 33.392332421538484 37.0 33.0 37.0 22.0 40.0 74-75 33.15990640285999 37.0 33.0 37.0 22.0 40.0 76-77 32.923472026336455 37.0 33.0 37.0 22.0 38.5 78-79 32.687919929502286 37.0 33.0 37.0 22.0 37.0 80-81 32.47752497440648 37.0 33.0 37.0 22.0 37.0 82-83 32.27077819820214 37.0 33.0 37.0 22.0 37.0 84-85 32.09644258902699 33.0 33.0 37.0 22.0 37.0 86-87 32.111795349211306 33.0 33.0 37.0 22.0 37.0 88-89 32.12511244568212 33.0 33.0 37.0 22.0 37.0 90-91 32.133119989332215 33.0 33.0 37.0 22.0 37.0 92-93 32.09187120903714 33.0 33.0 37.0 22.0 37.0 94-95 32.11748430699241 33.0 33.0 37.0 22.0 37.0 96-97 32.07701620840568 33.0 33.0 37.0 22.0 37.0 98-99 32.00587769014168 33.0 33.0 37.0 22.0 37.0 100 31.47303526084929 33.0 33.0 37.0 22.0 37.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.6042226327975833 1101 2 4.150025613782557 1101 3 2.40545402917855 1101 4 1.117055891768402 1101 5 0.5095365952467361 1101 6 0.5590174249938329 1101 7 0.6311737700716833 1101 8 0.6105317181915879 1101 9 0.6652671915118162 1101 10-11 0.6803323094402955 1101 12-13 0.6770611008279772 1101 14-15 1.042057491866295 1101 16-17 1.0106260035272214 1101 18-19 1.0166697809534426 1101 20-21 0.9627530680083325 1101 22-23 0.9705478505487832 1101 24-25 0.9284893385446438 1101 26-27 0.9834108624526792 1101 28-29 0.8731942590494413 1101 30-31 0.7337305468827182 1101 32-33 1.0383094890852362 1101 34-35 1.1161029165601946 1101 36-37 1.0390020478909463 1101 38-39 0.9909725631898354 1101 40-41 1.0418444101076147 1101 42-43 1.056690112003004 1101 44-45 1.106876786466124 1101 46-47 1.1219958804659171 1101 48-49 1.072256629813964 1101 50-51 1.0621437429318803 1101 52-53 0.9477509704273572 1101 54-55 1.013361380345252 1101 56-57 0.9537824960731385 1101 58-59 0.9555849605503326 1101 60-61 1.0424826110469425 1101 62-63 1.0398179498004012 1101 64-65 1.0317625030098938 1101 66-67 1.0295062102579067 1101 68-69 0.9509359820892982 1101 70-71 0.9884637528108939 1101 72-73 0.919094796513761 1101 74-75 0.8874373564934714 1101 76-77 0.8615230992305243 1101 78-79 0.9198883211868392 1101 80-81 0.9765259637154671 1101 82-83 0.9696239629489583 1101 84-85 0.991236162362739 1101 86-87 1.0127244996836957 1101 88-89 0.8772646881218442 1101 90-91 0.9580701415823363 1101 92-93 0.9924487831831463 1101 94-95 1.0200128781955904 1101 96-97 0.8891909189015301 1101 98-99 0.9057916422527477 1101 100 1.0655019840525135 1102 1 0.5857169364776027 1102 2 4.18781123408322 1102 3 2.3669176108708747 1102 4 1.1116597277971962 1102 5 0.5669872717459299 1102 6 0.5117220463006902 1102 7 0.5928247889935179 1102 8 0.5913603166366954 1102 9 0.6593440351807729 1102 10-11 0.6598579976659735 1102 12-13 0.7478683701286499 1102 14-15 1.011728562841597 1102 16-17 1.0634736856001155 1102 18-19 1.0790935471548764 1102 20-21 0.990376163445589 1102 22-23 0.9919435538533747 1102 24-25 1.0316597786677804 1102 26-27 0.966841434677093 1102 28-29 0.97859354697448 1102 30-31 0.9404173350758569 1102 32-33 1.0843917712314166 1102 34-35 1.1755804329516266 1102 36-37 1.031973443197927 1102 38-39 1.0173009079885489 1102 40-41 1.0363383249253175 1102 42-43 1.082354441765709 1102 44-45 1.1421217464095719 1102 46-47 1.0827533087152261 1102 48-49 0.9580594486460967 1102 50-51 0.9281135347646554 1102 52-53 1.0111949756532255 1102 54-55 1.043825175585468 1102 56-57 1.0470260483723237 1102 58-59 1.0126460170621883 1102 60-61 0.9758628386572852 1102 62-63 1.1574032803660046 1102 64-65 1.084607270946755 1102 66-67 1.0311028653892578 1102 68-69 1.0744968068690568 1102 70-71 1.0114421233938842 1102 72-73 1.0242103641548255 1102 74-75 0.9607935040149655 1102 76-77 0.9763701327332939 1102 78-79 0.9053719605277664 1102 80-81 0.9100771928281048 1102 82-83 0.9112975885724666 1102 84-85 0.8983057048387195 1102 86-87 0.9565745186605561 1102 88-89 0.9762731008828673 1102 90-91 0.9101483402079786 1102 92-93 1.0217509455187148 1102 94-95 0.9026332856337724 1102 96-97 0.9394412315300436 1102 98-99 0.9467494161885277 1102 100 0.917411535797779 1103 1 0.5491898715103645 1103 2 4.097345863000335 1103 3 2.3889335376818686 1103 4 1.170185581602773 1103 5 0.5205002274663428 1103 6 0.5690264674046261 1103 7 0.5923939626638628 1103 8 0.6097974004392697 1103 9 0.6527232607181404 1103 10-11 0.6575563591349294 1103 12-13 0.6182180663522203 1103 14-15 0.9726203255102561 1103 16-17 0.9335532239876443 1103 18-19 0.9405490170675179 1103 20-21 0.9429315642898572 1103 22-23 0.9370773908552863 1103 24-25 0.9397891525190332 1103 26-27 0.9685263556323065 1103 28-29 0.9959372339909578 1103 30-31 0.9760842880405676 1103 32-33 0.9733511781824404 1103 34-35 1.0965916930691932 1103 36-37 0.9287842865810418 1103 38-39 0.9405849303270699 1103 40-41 0.960476267293366 1103 42-43 0.9616969223158733 1103 44-45 1.0801633386258302 1103 46-47 1.0372318307270803 1103 48-49 0.8847791209492755 1103 50-51 0.9086934238937019 1103 52-53 0.9192297385047681 1103 54-55 0.9390359515200899 1103 56-57 0.9613512248523577 1103 58-59 0.9545234778578973 1103 60-61 0.8623489632381407 1103 62-63 0.9525622051296025 1103 64-65 0.8822470346352347 1103 66-67 0.9072750704382884 1103 68-69 0.9519311766506391 1103 70-71 0.9979372982328911 1103 72-73 0.9794987434120515 1103 74-75 0.9202143649656733 1103 76-77 0.8366142795874083 1103 78-79 0.8909663500250531 1103 80-81 0.895626404399195 1103 82-83 0.9165063730981444 1103 84-85 0.9130548818051878 1103 86-87 0.9837297977313106 1103 88-89 0.9471184894887479 1103 90-91 0.8786714601547736 1103 92-93 0.9336423831528116 1103 94-95 0.9424994527402752 1103 96-97 0.92923539274015 1103 98-99 0.968784491162463 1103 100 0.9243495094837151 1104 1 0.42474283353597997 1104 2 3.687848474877214 1104 3 2.16679634701795 1104 4 0.8967765808694566 1104 5 0.29826179403787023 1104 6 0.33492395639761696 1104 7 0.3623427899361573 1104 8 0.3088132562518169 1104 9 0.40858670686854737 1104 10-11 0.4204175566068784 1104 12-13 0.45148696260691423 1104 14-15 0.5253100965787851 1104 16-17 0.6453144666178403 1104 18-19 0.6048629461098116 1104 20-21 0.5881583911424428 1104 22-23 0.5936848100351639 1104 24-25 0.5902987889450486 1104 26-27 0.550402897523945 1104 28-29 0.5656650732563975 1104 30-31 0.49580383936791605 1104 32-33 0.5988730733878 1104 34-35 0.782147159084964 1104 36-37 0.704481859142966 1104 38-39 0.6202015952116611 1104 40-41 0.5566382342902401 1104 42-43 0.56463153011439 1104 44-45 0.6858962242260347 1104 46-47 0.6755742383735353 1104 48-49 0.4972679560564188 1104 50-51 0.45290585210745604 1104 52-53 0.49733446211453014 1104 54-55 0.5392567442032856 1104 56-57 0.46416250943422455 1104 58-59 0.6093855173524076 1104 60-61 0.6308361480379077 1104 62-63 0.654431147747033 1104 64-65 0.6245386085293276 1104 66-67 0.6286578382568493 1104 68-69 0.6454476799759234 1104 70-71 0.5818841081669035 1104 72-73 0.6215214483671758 1104 74-75 0.5345848919983354 1104 76-77 0.5285148051530655 1104 78-79 0.5073625461973634 1104 80-81 0.5641237223537701 1104 82-83 0.6423696733476874 1104 84-85 0.5934105951954152 1104 86-87 0.6535429608828842 1104 88-89 0.6196033843849413 1104 90-91 0.589200595209217 1104 92-93 0.5781639140203474 1104 94-95 0.6065701742294785 1104 96-97 0.5683850494040605 1104 98-99 0.5984433119242674 1104 100 0.5553096001541391 1105 1 0.5106123141455825 1105 2 3.7736618082433324 1105 3 2.233207675459095 1105 4 1.0503840807828766 1105 5 0.39854636220218964 1105 6 0.35234611232823454 1105 7 0.3018479992323293 1105 8 0.27036351852940754 1105 9 0.2777722653408503 1105 10-11 0.3676909917212896 1105 12-13 0.35543713488497986 1105 14-15 0.5099401253248317 1105 16-17 0.4916498973346819 1105 18-19 0.5268800454922697 1105 20-21 0.47327919890397396 1105 22-23 0.4704324917877898 1105 24-25 0.5745517997824336 1105 26-27 0.4419999443897069 1105 28-29 0.4825939970229314 1105 30-31 0.39401092146739103 1105 32-33 0.4777268915218471 1105 34-35 0.5924996422875566 1105 36-37 0.5239003071892512 1105 38-39 0.4913939053460794 1105 40-41 0.47019722567848987 1105 42-43 0.5627145467119945 1105 44-45 0.5453148262527634 1105 46-47 0.5355496875668564 1105 48-49 0.48344886944209264 1105 50-51 0.5169391486523338 1105 52-53 0.43332178899667184 1105 54-55 0.42706451079563124 1105 56-57 0.35350604072289116 1105 58-59 0.41419195265788034 1105 60-61 0.3731092788506558 1105 62-63 0.4776168914057095 1105 64-65 0.37969915641987484 1105 66-67 0.35557251707543713 1105 68-69 0.40062261596352045 1105 70-71 0.33733202280178176 1105 72-73 0.413790523288057 1105 74-75 0.39446552319287775 1105 76-77 0.27835377702503195 1105 78-79 0.3509696541339409 1105 80-81 0.3565629222514488 1105 82-83 0.3686458748701611 1105 84-85 0.381098971319652 1105 86-87 0.37529379118440076 1105 88-89 0.3160120919383296 1105 90-91 0.3473037065481144 1105 92-93 0.4041881965029255 1105 94-95 0.3515436859330556 1105 96-97 0.3819215906770701 1105 98-99 0.4401904911912631 1105 100 0.3658993625444502 1106 1 0.5490028447569948 1106 2 3.916739138350785 1106 3 2.135353583601468 1106 4 0.958856100607747 1106 5 0.28597487092150686 1106 6 0.13913948327982695 1106 7 0.1473186230471839 1106 8 0.11404110742189744 1106 9 0.17663418965685906 1106 10-11 0.08627204664887955 1106 12-13 0.10611493560348606 1106 14-15 0.23130673490906162 1106 16-17 0.20487332784425405 1106 18-19 0.24086200880469022 1106 20-21 0.15968188271408223 1106 22-23 0.14151790020791566 1106 24-25 0.07554725445549337 1106 26-27 0.15412800470142685 1106 28-29 0.06448976330992195 1106 30-31 0.0714280987708733 1106 32-33 0.15792145669578161 1106 34-35 0.2989882815223268 1106 36-37 0.2001031004002698 1106 38-39 0.07723388908056705 1106 40-41 0.1487236397022258 1106 42-43 0.20010592207534472 1106 44-45 0.24492864785782587 1106 46-47 0.12363932314953985 1106 48-49 0.07258785197963391 1106 50-51 0.015023909684522607 1106 52-53 0.03612725019466012 1106 54-55 0.07133980635246928 1106 56-57 0.17326294272060494 1106 58-59 0.08535532836493331 1106 60-61 0.1076490847023237 1106 62-63 0.12930333275414796 1106 64-65 0.05994701838076821 1106 66-67 0.04428908110431706 1106 68-69 0.0023430855877606405 1106 70-71 0.04593428585226178 1106 72-73 0.04104453500909244 1106 74-75 0.048141767968473914 1106 76-77 0.06790513972987355 1106 78-79 0.026961216822961376 1106 80-81 0.07430496750395577 1106 82-83 0.10286938789830913 1106 84-85 0.026587459979303674 1106 86-87 0.18361464039405107 1106 88-89 0.16512106153662032 1106 90-91 0.18541286585404393 1106 92-93 0.13286059182982157 1106 94-95 0.1311614718322609 1106 96-97 0.0799648372127777 1106 98-99 0.10278154873052614 1106 100 0.08007826450303313 1107 1 0.39380183552110637 1107 2 3.6328112030483304 1107 3 2.131140124821055 1107 4 0.8687705968361712 1107 5 0.2526643599284668 1107 6 0.1899220537490649 1107 7 0.03183269668333821 1107 8 -0.010019029023695225 1107 9 0.07035459325015836 1107 10-11 -0.015120200324211908 1107 12-13 0.059052490817130376 1107 14-15 -0.04175153448381508 1107 16-17 -0.021271347265837903 1107 18-19 0.025303488871351476 1107 20-21 -0.033955189861799795 1107 22-23 -0.02414803631229745 1107 24-25 0.020004625945084342 1107 26-27 0.001342921248294715 1107 28-29 -0.062225091338717675 1107 30-31 -0.016348348405522017 1107 32-33 0.08009731840756018 1107 34-35 0.18327863727292737 1107 36-37 0.008722491978573998 1107 38-39 -0.07024036841438175 1107 40-41 -0.05932982520906194 1107 42-43 0.0255380938273575 1107 44-45 0.0869836101167536 1107 46-47 -0.004744130906395583 1107 48-49 -0.07377303743476205 1107 50-51 -0.1103736074806605 1107 52-53 -0.14486241406429912 1107 54-55 -0.021378351219368597 1107 56-57 -0.09123787543082074 1107 58-59 0.034244360419791064 1107 60-61 0.0219601237251581 1107 62-63 0.07558716518932584 1107 64-65 0.021224328342675847 1107 66-67 0.013247329696028487 1107 68-69 0.02957729585477864 1107 70-71 0.021791871080232283 1107 72-73 -0.10277974812940727 1107 74-75 -0.15235194409780206 1107 76-77 -0.021087333950752907 1107 78-79 -0.05655831648965659 1107 80-81 0.09268221489391237 1107 82-83 0.016744444427814642 1107 84-85 -0.04308063918923466 1107 86-87 0.0391075397829006 1107 88-89 -0.03652917894010699 1107 90-91 -0.06154628889405345 1107 92-93 0.10445621027735186 1107 94-95 -0.00849714469600471 1107 96-97 0.03998030755931836 1107 98-99 -0.0010029595322578189 1107 100 0.025638729809287497 1108 1 0.4184055238279356 1108 2 3.598196640596072 1108 3 2.0838120968482414 1108 4 0.930300703868518 1108 5 0.18147981553223502 1108 6 0.0448962154042718 1108 7 0.057448559149896994 1108 8 -0.011252688946797207 1108 9 0.059813880645663176 1108 10-11 0.12215097880553571 1108 12-13 0.08687229026579502 1108 14-15 0.06133372815079241 1108 16-17 0.04751418326108592 1108 18-19 -0.02967725839354074 1108 20-21 -0.03992044009919482 1108 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0.021042783648205443 2211 82-83 -0.3072243524391922 2211 84-85 0.0440355056101609 2211 86-87 0.11949338378584784 2211 88-89 0.3340343288905636 2211 90-91 0.3298513329938082 2211 92-93 0.2948833536294835 2211 94-95 0.26928664832334803 2211 96-97 0.15571757958061383 2211 98-99 0.10916631565335067 2211 100 -0.03671907889228265 2212 1 0.5605712806009926 2212 2 4.207824942871607 2212 3 2.436627943654301 2212 4 1.1299027540553936 2212 5 0.6794526436745087 2212 6 0.513349425375452 2212 7 0.3816560327725469 2212 8 0.3496189336202278 2212 9 0.2535565611113597 2212 10-11 0.4360680162177246 2212 12-13 0.40830398026037784 2212 14-15 0.4727969634800715 2212 16-17 0.4748511274780469 2212 18-19 0.44522259925431484 2212 20-21 0.5482077404483192 2212 22-23 0.47338575637567715 2212 24-25 0.5004332219476666 2212 26-27 0.5024380993323376 2212 28-29 0.362450196902266 2212 30-31 0.43725856218507175 2212 32-33 0.5591109050915222 2212 34-35 0.62042349408609 2212 36-37 0.47664929898286346 2212 38-39 0.3881135443318513 2212 40-41 0.5086009051989961 2212 42-43 0.49974118671404 2212 44-45 0.7441579072236735 2212 46-47 0.5825878838679586 2212 48-49 0.38252989489822653 2212 50-51 0.4637251988026492 2212 52-53 0.5198495115960569 2212 54-55 0.4369895360160996 2212 56-57 0.5775362496791487 2212 58-59 0.5130588238157472 2212 60-61 0.5521177042844059 2212 62-63 0.7562651293604219 2212 64-65 0.6763986914724711 2212 66-67 0.802485348786341 2212 68-69 0.7614409664331419 2212 70-71 0.6797347123607196 2212 72-73 0.5287729821535692 2212 74-75 0.7293379207483213 2212 76-77 0.6452589741421235 2212 78-79 0.5926664474021166 2212 80-81 0.5161910815227557 2212 82-83 0.671272447880412 2212 84-85 0.7792560894703726 2212 86-87 0.46758325628659847 2212 88-89 0.589278612454617 2212 90-91 0.5453424171135985 2212 92-93 0.7084706538875416 2212 94-95 0.7600624799144136 2212 96-97 0.5119633550800664 2212 98-99 0.70721903930907 2212 100 0.49729619809800596 2213 1 -0.6872065160754808 2213 2 -25.683930878399632 2213 3 -14.660138678222452 2213 4 -6.084500321140631 2213 5 -2.1334213379219378 2213 6 -1.4747290873726655 2213 7 -1.1900412781318082 2213 8 -1.2159330878789305 2213 9 -1.271215095378352 2213 10-11 -1.1428112721329313 2213 12-13 -1.2209809686280906 2213 14-15 -1.4538005906505234 2213 16-17 -1.4663408511588685 2213 18-19 -1.4773345078842866 2213 20-21 -1.5401318578031749 2213 22-23 -1.5306589428232016 2213 24-25 -1.3951453333225246 2213 26-27 -1.6194684201512786 2213 28-29 -1.6216720625503882 2213 30-31 -1.4163149924898093 2213 32-33 -1.433715621875976 2213 34-35 -1.4406296925177529 2213 36-37 -1.4548706098498272 2213 38-39 -1.6314100384075871 2213 40-41 -1.4271431459635622 2213 42-43 -1.3898247480060775 2213 44-45 -1.359405442154653 2213 46-47 -1.5350553729823417 2213 48-49 -1.5875464747376071 2213 50-51 -1.4521329815929747 2213 52-53 -1.6980174113369415 2213 54-55 -1.5042653752828627 2213 56-57 -1.4492815687686331 2213 58-59 -1.5477222215467918 2213 60-61 -1.507586682292235 2213 62-63 -1.492431144005458 2213 64-65 -1.3293741046086964 2213 66-67 -1.4316853858249274 2213 68-69 -1.5060764250930632 2213 70-71 -1.5419455897160539 2213 72-73 -1.5699981300536692 2213 74-75 -1.5537774161115117 2213 76-77 -1.45167603512769 2213 78-79 -1.440782262478308 2213 80-81 -1.3708328839153054 2213 82-83 -1.325313029982599 2213 84-85 -1.379432019658264 2213 86-87 -1.16956147182729 2213 88-89 -1.3431555208605666 2213 90-91 -1.2888257661735345 2213 92-93 -1.2281604076333856 2213 94-95 -1.326975092585716 2213 96-97 -1.5361724774170007 2213 98-99 -1.408956355720946 2213 100 -1.4762380661851573 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 13.0 4 32.0 5 46.0 6 42.0 7 47.0 8 52.0 9 95.0 10 410.0 11 2109.0 12 6149.0 13 11242.0 14 15126.0 15 16822.0 16 17486.0 17 18373.0 18 18539.0 19 19381.0 20 21018.0 21 23365.0 22 25315.0 23 27151.0 24 28429.0 25 29860.0 26 32794.0 27 37118.0 28 43595.0 29 51024.0 30 61858.0 31 74961.0 32 94168.0 33 124535.0 34 169660.0 35 239339.0 36 363530.0 37 619593.0 38 636462.0 39 4961.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 38.62430658387766 20.63066358849812 10.412898955828329 30.332130871795894 2 12.617552761889256 22.117651315320856 37.03524896330458 28.229546959485308 3 15.351172715862196 26.696880306995237 28.716739890118966 29.235207087023607 4 10.251573369892126 18.239977759910758 37.9161417396449 33.59230713055222 5 11.419870225053025 38.523836481587885 35.48891060992529 14.567382683433799 6 30.001644854112737 39.81754120430147 17.48663467790156 12.694179263684232 7 26.113559278369614 32.971313565348304 23.14063294583396 17.77449421044812 8 24.37404644540965 37.366634703581504 20.602143585425257 17.65717526558359 9 24.43025390162481 16.788183090941626 21.83875836906948 36.94280463836409 10-11 22.996862148032783 27.857568131197574 28.981968027155425 20.16360169361422 12-13 23.760200712686736 25.127395312445405 29.475493761608863 21.636910213258993 14-15 21.212703008605445 26.612607506284764 26.494111925472897 25.68057755963689 16-17 20.562613897668193 30.4485256648236 27.459660286750392 21.529200150757813 18-19 20.623384038401618 29.395583164745776 29.448514894459425 20.532517902393185 20-21 21.679065762106386 28.704075020187826 28.68893746159374 20.927921756112053 22-23 20.80688818780167 28.684204119179498 28.94667663604693 21.562231056971903 24-25 21.082376403286183 28.43017720046865 28.746890774923244 21.74055562132192 26-27 20.99973044814055 28.462630021726444 28.822908662140733 21.71473086799227 28-29 20.695559177273363 28.385783750044546 29.29808968303226 21.620567389649832 30-31 21.480338093611593 28.785028129772392 28.559622979187072 21.175010797428943 32-33 20.40245369685234 28.717414760104514 29.365199336852037 21.51493220619111 34-35 20.533613961213522 28.298194875036863 29.417931161971584 21.75026000177803 36-37 21.230335683361464 28.767600515914904 28.701805843272503 21.300257957451134 38-39 20.989151951354003 28.716388918190354 28.94743340933682 21.347025721118822 40-41 21.127820689489724 28.50075604109646 28.788778416808313 21.5826448526055 42-43 20.847153473096746 28.623710764635707 29.02949940915099 21.499636353116557 44-45 21.242729599306813 28.141680996373886 28.900085307916417 21.715504096402885 46-47 21.151443494264164 28.796760566212885 28.80918030103038 21.242615638492566 48-49 21.05369110347158 28.593399477935773 28.88583660395368 21.46707281463897 50-51 21.251180516263823 28.568802331516313 28.468486015789146 21.711531136430718 52-53 20.79427507657856 28.72781577176951 28.61328977899183 21.864619372660098 54-55 21.181149359824968 28.669139186044646 28.302054569388364 21.84765688474203 56-57 21.31680316842862 28.511694165486883 28.329738860441832 21.84176380564266 58-59 20.857018259106354 28.137885872734902 29.014553020421225 21.990542847737522 60-61 21.445095862127605 28.588386465800923 28.2435431853231 21.722974486748374 62-63 20.716016516401286 28.821490727827808 28.683477688656485 21.77901506711442 64-65 20.745931191388372 28.24488501200725 28.83369170416799 22.17549209243639 66-67 21.08032160468551 28.56631230967286 28.45784756045172 21.895518525189914 68-69 21.254739072256914 28.439426681647262 28.519087557447577 21.786746688648247 70-71 21.316642074513254 28.38028602647554 28.279841356350627 22.02323054266058 72-73 21.022767570916876 28.575854441110703 28.69076322509595 21.71061476287647 74-75 21.386466354843883 28.051947410383587 28.409342121675813 22.152244113096717 76-77 21.12518918781598 28.568132047847072 28.52505041627053 21.781628348066416 78-79 21.185234789929073 28.45557054282657 28.613724866869873 21.74546980037449 80-81 21.22153515911944 28.28543061638804 28.43285751684904 22.06017670764348 82-83 20.73301720924678 28.602326636115023 28.58297534023971 22.081680814398492 84-85 20.936278383832683 28.152404775681283 28.395757772477737 22.515559068008297 86-87 21.138271473833893 28.375077021916827 28.339267232931743 22.14738427131754 88-89 20.837120036126556 28.10032175213796 28.692917219384157 22.369640992351332 90-91 21.150664536949737 28.142974447806797 28.426799506437217 22.27956150880625 92-93 20.607718375805145 28.568969154578483 28.583309873117273 22.2400025964991 94-95 20.562124645812403 28.05211637673143 28.76407225427001 22.621686723186166 96-97 20.920744163042354 28.248357968436938 28.523027952592088 22.30786991592862 98-99 20.777878866878588 28.063667083762102 28.60580883294534 22.552645216413968 100 20.88812658888523 27.903852734374585 28.44930451895821 22.758716157781976 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 16.0 1 14.0 2 14.5 3 23.0 4 29.0 5 34.0 6 49.5 7 86.5 8 142.0 9 168.0 10 171.0 11 179.0 12 214.0 13 278.0 14 347.0 15 408.0 16 483.5 17 653.0 18 888.0 19 1151.0 20 1619.0 21 2341.0 22 3432.0 23 5024.0 24 7498.5 25 11007.5 26 15895.5 27 22333.5 28 30195.5 29 39568.0 30 48964.5 31 58423.5 32 68431.5 33 77385.5 34 85670.5 35 93511.0 36 100611.5 37 106964.5 38 111664.0 39 114991.5 40 116898.0 41 116647.0 42 114360.0 43 111540.5 44 107935.0 45 103129.5 46 98560.0 47 94270.5 48 90068.0 49 88747.0 50 96647.0 51 92561.0 52 74903.5 53 65538.5 54 60518.5 55 56102.5 56 51469.0 57 46770.0 58 42169.5 59 37537.0 60 32504.5 61 27094.0 62 22279.5 63 17992.0 64 14025.0 65 11128.0 66 8608.5 67 6386.0 68 4735.5 69 3532.0 70 2591.0 71 1933.5 72 1476.5 73 1085.0 74 782.5 75 531.0 76 325.0 77 201.0 78 119.5 79 54.0 80 30.5 81 17.5 82 11.0 83 6.5 84 3.0 85 1.0 86 0.5 87 1.0 88 0.5 89 0.0 90 0.0 91 0.0 92 0.5 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content warn #Base N-Count 1 0.9667682881657402 2 6.396404278121651 3 0.0 4 0.0063851508906403575 5 0.17215213451008254 6 0.05728997263204387 7 0.03749953257873315 8 0.04321441901123998 9 0.04243832332287486 10-11 0.043567189778678676 12-13 0.04229721501589938 14-15 0.04455494792750702 16-17 0.035912064125259026 18-19 0.03044411722995927 20-21 0.02439409856838567 22-23 0.024376460030013737 24-25 0.021007499200974212 26-27 0.012981964241743929 28-29 0.019931548360286194 30-31 0.024482291260245346 32-33 0.0014287216081267098 34-35 0.0037393701348501537 36-37 0.004444911669727541 38-39 0.006455705044128095 40-41 0.007055415348773874 42-43 0.007813872498767067 44-45 0.009313148260381515 46-47 0.017744369602166295 48-49 0.02679293978696879 50-51 0.023512171649788936 52-53 0.029897322540429296 54-55 0.02647544609627396 56-57 0.017268129066124058 58-59 0.01569829915102187 60-61 0.013087795471975538 62-63 0.019384753670756222 64-65 0.020460704511444237 66-67 0.02121916166143743 68-69 0.02871554046950967 70-71 0.02834513116369904 72-73 0.0252231098718666 74-75 0.025417133793957887 76-77 0.019314199517268482 78-79 0.01569829915102187 80-81 0.014675263925449659 82-83 0.009401340952241188 84-85 0.012064760246403326 86-87 0.00993049710339923 88-89 0.007831511037139 90-91 0.007390547577840634 92-93 0.004092140902288847 94-95 0.0016227455302179911 96-97 9.701196104564078E-4 98-99 6.70264458133518E-4 100 0.10304434116884245 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 2834702.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 85.47169346519074 #Duplication Level Percentage of deduplicated Percentage of total 1 90.66494926867075 77.49286751928895 2 7.23528702502437 12.368244694711095 3 1.2356830301721977 3.1684776352504835 4 0.37059621041886304 1.2670194278512954 5 0.15839282892548853 0.6769051660501876 6 0.08087803538512099 0.41476695891023463 7 0.05301805529559489 0.31720800792419335 8 0.040934097383771566 0.27989652990879915 9 0.025951149931852796 0.19962798588400746 >10 0.1257351224757972 1.8517896911557814 >50 0.0056661527681602 0.33251756229079826 >100 0.0019152448840772521 0.3523680358616166 >500 4.968893323832929E-4 0.25432435315115387 >1k 4.140744429385703E-4 0.581767841747915 >5k 8.281488858771407E-5 0.4422185900133956 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 7178 0.25321885686749435 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 5317 0.18756821704715346 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 3.527707674386937E-5 0.0 7 0.0 0.0 0.0 7.055415348773874E-5 0.0 8 0.0 0.0 0.0 7.055415348773874E-5 0.0 9 0.0 0.0 0.0 7.055415348773874E-5 0.0 10-11 0.0 0.0 0.0 1.0583123023160811E-4 0.0 12-13 0.0 0.0 0.0 1.5874684534741216E-4 0.0 14-15 0.0 0.0 0.0 2.1166246046321624E-4 0.0 16-17 0.0 0.0 0.0 2.1166246046321624E-4 0.0 18-19 0.0 0.0 0.0 2.8221661395095497E-4 0.0 20-21 0.0 0.0 0.0 3.5277076743869373E-4 0.0 22-23 0.0 0.0 0.0 3.880478441825631E-4 0.0 24-25 0.0 0.0 0.0 3.880478441825631E-4 0.0 26-27 0.0 0.0 0.0 4.7624053604223654E-4 0.0 28-29 0.0 0.0 0.0 8.819269185967344E-4 0.0 30-31 3.527707674386937E-5 0.0 0.0 0.0031925754453201783 0.0 32-33 3.527707674386937E-5 3.527707674386937E-5 0.0 0.008678160878991866 0.0 34-35 3.527707674386937E-5 3.527707674386937E-5 0.0 0.017021189528916972 0.0 36-37 3.527707674386937E-5 3.527707674386937E-5 0.0 0.029862045463685424 0.0 38-39 3.527707674386937E-5 3.527707674386937E-5 0.0 0.05072843635768416 0.0 40-41 3.527707674386937E-5 3.527707674386937E-5 0.0 0.07930286852021835 0.0 42-43 3.527707674386937E-5 7.055415348773874E-5 0.0 0.10789493922112448 0.0 44-45 3.527707674386937E-5 7.055415348773874E-5 0.0 0.14133760797431266 0.0 46-47 7.055415348773874E-5 7.055415348773874E-5 0.0 0.18070682562047086 0.0 48-49 7.055415348773874E-5 7.055415348773874E-5 0.0 0.22141657218289612 0.0 50-51 7.055415348773874E-5 7.055415348773874E-5 0.0 0.26701219387434727 0.0 52-53 7.055415348773874E-5 7.055415348773874E-5 0.0 0.316241354470417 0.0 54-55 7.055415348773874E-5 7.055415348773874E-5 0.0 0.3730198094896747 0.0 56-57 7.055415348773874E-5 7.055415348773874E-5 0.0 0.43665965593561507 0.0 58-59 7.055415348773874E-5 1.0583123023160812E-4 0.0 0.5115352513244779 0.0 60-61 7.055415348773874E-5 1.0583123023160812E-4 0.0 0.5872927736319373 0.0 62-63 7.055415348773874E-5 1.0583123023160812E-4 0.0 0.6651845590824008 0.0 64-65 7.055415348773874E-5 1.0583123023160812E-4 0.0 0.7550176350106643 0.0 66-67 7.055415348773874E-5 1.234697686035428E-4 0.0 0.8586087708690366 0.0 68-69 7.055415348773874E-5 1.4110830697547749E-4 0.0 0.9719187413703452 0.0 70-71 7.055415348773874E-5 1.5874684534741216E-4 0.0 1.1055306695377505 0.0 72-73 7.055415348773874E-5 1.7638538371934686E-4 0.0 1.247362156586477 0.0 74-75 7.055415348773874E-5 1.7638538371934686E-4 0.0 1.3864067545724383 0.0 76-77 7.055415348773874E-5 1.7638538371934686E-4 0.0 1.54478319061404 0.0 78-79 7.055415348773874E-5 1.7638538371934686E-4 0.0 1.7215213451008253 0.0 80-81 7.055415348773874E-5 1.7638538371934686E-4 0.0 1.9244527290699338 0.0 82-83 7.055415348773874E-5 1.7638538371934686E-4 0.0 2.149926165078375 0.0 84-85 7.055415348773874E-5 1.7638538371934686E-4 0.0 2.369349582425243 0.0 86-87 7.055415348773874E-5 1.7638538371934686E-4 0.0 2.605141563381266 0.0 88 7.055415348773874E-5 2.1166246046321624E-4 0.0 2.8038573366794814 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CCTATAC 945 0.0 19.409191 3 GGGTACG 175 0.0014833455 17.239773 1 CTAGGAC 1190 0.0 16.994022 3 GTCTAGG 955 0.0 16.848644 1 GTACTAC 795 0.0 16.444647 1 GAGTACT 3380 0.0 16.317144 12-13 CTACGTA 205 3.6501623E-4 16.027834 9 GTTAGAC 605 0.0 15.547097 3 GTATCAA 11630 0.0 15.478243 1 CATGGGG 3725 0.0 15.277117 4 TAGGACC 740 0.0 14.61768 4 TACCGTT 225 7.3985907E-4 14.604169 7 TACACCG 325 3.7404789E-6 14.471015 5 CGTACCC 195 0.004768344 14.470759 3 CTATACT 1405 0.0 14.393767 4 TATCAAC 12570 0.0 14.227844 2 CAACGCA 12265 0.0 14.226192 5 TCTACAC 1390 0.0 14.210492 3 AACGCAG 12305 0.0 14.156916 6 CGTAGAG 300 2.8222632E-5 14.10899 3 >>END_MODULE