FastQCFastQC Report
Wed 25 May 2016
SRR1295087_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295087_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2244321
Sequences flagged as poor quality0
Sequence length100
%GC42

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGTAC4950.021.5772173
TATAGCG1850.00230662816.1379747
CATGGGT15850.015.605244
GTATTAG9600.015.55717851
GTACATG85450.015.4388371
GTATAGA9700.015.3967941
TACATGG86950.014.8832872
GTATAGG6909.276846E-1114.4298461
ACATGGG90000.014.2409633
TGTACTG10550.014.2381265
TCTATAC7950.014.1419713
GTATAAC5802.9373041E-813.9478141
ACAGCGT3303.8063212E-513.6672288
GTACTGT9000.013.3536116
GTATATA18650.013.346641
GTATTAA14950.013.3198591
GTACTGA7202.7157512E-912.9643141
GTACAAG9203.6379788E-1212.8515821
GTATTAC7353.6925485E-912.6997371
GTATTAT12450.012.4957251
Produced by FastQC (version 0.11.5)