##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1295087_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2244321 Sequences flagged as poor quality 0 Sequence length 100 %GC 42 >>END_MODULE >>Per base sequence quality warn #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 25.932817988157666 27.0 22.0 33.0 6.0 33.0 2 22.027158325391063 27.0 6.0 33.0 2.0 33.0 3 24.433734300931107 27.0 15.0 33.0 15.0 33.0 4 24.527584066628616 33.0 6.0 37.0 2.0 37.0 5 26.45041997111821 33.0 15.0 37.0 2.0 37.0 6 26.994525738519577 33.0 15.0 37.0 6.0 37.0 7 26.939362952091077 33.0 15.0 37.0 6.0 37.0 8 27.69115246883133 33.0 15.0 37.0 6.0 37.0 9 28.726674125492742 33.0 22.0 37.0 15.0 37.0 10-11 28.235174914818334 33.0 18.5 37.0 10.5 37.0 12-13 27.07537134839446 33.0 15.0 37.0 4.0 37.0 14-15 28.741680000320812 33.0 18.5 37.0 6.0 40.0 16-17 29.120793995154884 33.0 22.0 37.0 6.0 40.0 18-19 29.269777139722883 33.0 22.0 37.0 6.0 40.0 20-21 28.79442624294831 33.0 22.0 37.0 6.0 40.0 22-23 29.497705987690708 33.0 24.5 37.0 6.0 40.0 24-25 29.63996928246895 33.0 24.5 37.0 10.5 40.0 26-27 28.2068037504439 33.0 18.5 37.0 4.0 40.0 28-29 28.915882353727476 33.0 22.0 37.0 6.0 40.0 30-31 29.580917346493663 33.0 24.5 37.0 10.5 40.0 32-33 28.422211662235483 33.0 22.0 37.0 6.0 40.0 34-35 28.024671381678466 33.0 15.0 37.0 6.0 40.0 36-37 28.333139288007374 33.0 22.0 37.0 6.0 40.0 38-39 28.43086327668814 33.0 22.0 37.0 6.0 40.0 40-41 27.483251950144385 33.0 15.0 37.0 6.0 40.0 42-43 28.07780192762087 33.0 18.5 37.0 6.0 40.0 44-45 27.926648861727 33.0 22.0 37.0 6.0 40.0 46-47 27.632379681872603 33.0 18.5 37.0 6.0 40.0 48-49 27.539725155180562 33.0 18.5 37.0 6.0 40.0 50-51 27.52353896791056 33.0 22.0 37.0 6.0 40.0 52-53 27.713020107195007 33.0 22.0 37.0 6.0 40.0 54-55 27.683676711130005 33.0 22.0 37.0 6.0 40.0 56-57 27.666751547572737 33.0 22.0 37.0 6.0 40.0 58-59 27.537190981147525 33.0 22.0 37.0 6.0 40.0 60-61 27.525204282275126 33.0 22.0 37.0 6.0 40.0 62-63 27.467080021084328 33.0 22.0 37.0 6.0 40.0 64-65 27.04447581250632 33.0 22.0 37.0 6.0 40.0 66-67 27.24343754748095 33.0 22.0 37.0 6.0 38.5 68-69 26.86025506155314 33.0 22.0 37.0 6.0 37.0 70-71 26.969893789702986 30.0 22.0 37.0 6.0 37.0 72-73 27.012741938430374 27.0 22.0 37.0 6.0 37.0 74-75 27.10491480496774 27.0 22.0 37.0 6.0 37.0 76-77 26.86904123786214 27.0 22.0 35.0 6.0 37.0 78-79 26.45922998537197 27.0 22.0 33.0 6.0 37.0 80-81 26.26273046502706 27.0 22.0 33.0 6.0 37.0 82-83 26.367641928226845 27.0 22.0 33.0 6.0 37.0 84-85 26.321153257488568 27.0 22.0 33.0 6.0 37.0 86-87 26.112628719332037 27.0 22.0 33.0 6.0 37.0 88-89 26.06048265822937 27.0 22.0 33.0 6.0 37.0 90-91 26.192816669273245 27.0 22.0 33.0 6.0 37.0 92-93 26.10973185208355 27.0 22.0 33.0 6.0 37.0 94-95 25.979873868310282 27.0 22.0 33.0 6.0 37.0 96-97 26.05837823555543 27.0 22.0 33.0 6.0 37.0 98-99 26.1179514873318 27.0 22.0 33.0 10.5 37.0 100 25.7792931581534 27.0 22.0 33.0 6.0 37.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 7.193728985079886 1101 2 11.045247856032454 1101 3 8.298835890972626 1101 4 12.571751786501537 1101 5 10.533534241531868 1101 6 9.804990440971924 1101 7 9.202921099134965 1101 8 8.783408398579265 1101 9 7.133138986259102 1101 10-11 7.5806333974473965 1101 12-13 8.856196548724721 1101 14-15 7.631655466448404 1101 16-17 7.821626207776113 1101 18-19 7.8714279824347635 1101 20-21 7.000428041594532 1101 22-23 7.133292212742468 1101 24-25 7.054169258693491 1101 26-27 8.212962734432079 1101 28-29 7.994080795115238 1101 30-31 5.295950292659381 1101 32-33 6.66039064311558 1101 34-35 8.495116261265082 1101 36-37 7.963808728565461 1101 38-39 8.019397187586645 1101 40-41 8.795492126213556 1101 42-43 8.32661469178898 1101 44-45 8.170539454418194 1101 46-47 7.056939273424174 1101 48-49 5.171961112299819 1101 50-51 6.549687161464881 1101 52-53 5.739173656194648 1101 54-55 3.860677939544452 1101 56-57 5.878968838679789 1101 58-59 6.022793819647891 1101 60-61 6.320793318629665 1101 62-63 5.94485391441275 1101 64-65 6.432997285122674 1101 66-67 5.490416193151265 1101 68-69 6.419069488231887 1101 70-71 6.012260026502389 1101 72-73 5.231973489996534 1101 74-75 4.705040437545563 1101 76-77 4.905006765686565 1101 78-79 5.035127597956624 1101 80-81 4.628496791929678 1101 82-83 4.127328830083908 1101 84-85 4.0440688165864245 1101 86-87 3.6996420744014173 1101 88-89 3.6872636860970367 1101 90-91 3.626700785904351 1101 92-93 3.4624185209854907 1101 94-95 3.410238509133759 1101 96-97 3.529739868312621 1101 98-99 3.364455677719537 1101 100 3.5311669444890335 1102 1 6.630669449994194 1102 2 10.315405401049869 1102 3 8.044832359254748 1102 4 12.31486746998467 1102 5 10.383511211915732 1102 6 9.160510161345282 1102 7 6.368851608692214 1102 8 7.378597946176953 1102 9 6.189668192726032 1102 10-11 7.15443626121289 1102 12-13 8.836794713462393 1102 14-15 6.0749567045389306 1102 16-17 7.020117801923718 1102 18-19 7.110147253539335 1102 20-21 6.55764322713129 1102 22-23 6.25831782245772 1102 24-25 6.7280792242009255 1102 26-27 8.21587516476303 1102 28-29 7.281036400578216 1102 30-31 4.01232367389159 1102 32-33 4.155962861492451 1102 34-35 4.906521140959356 1102 36-37 4.8659201082562475 1102 38-39 4.452299861147406 1102 40-41 5.913110917462195 1102 42-43 6.593646958925046 1102 44-45 6.187036382867582 1102 46-47 5.301747684700306 1102 48-49 3.643461431007946 1102 50-51 4.7379627320323365 1102 52-53 5.522262045884656 1102 54-55 5.113949033406165 1102 56-57 6.070081328110735 1102 58-59 5.75833521200369 1102 60-61 5.641403150345994 1102 62-63 4.072160999084378 1102 64-65 5.926155644380266 1102 66-67 3.61908543838684 1102 68-69 5.754236477177162 1102 70-71 5.1313150658397575 1102 72-73 4.942685551445567 1102 74-75 4.1132880112747685 1102 76-77 3.18840691381061 1102 78-79 4.298666423645127 1102 80-81 4.246408044001196 1102 82-83 3.5829341442889735 1102 84-85 2.7202687794533986 1102 86-87 2.899209255336668 1102 88-89 3.0436512038920718 1102 90-91 2.8490337285248195 1102 92-93 2.85071004821204 1102 94-95 2.751719004859634 1102 96-97 2.9697649968627644 1102 98-99 2.812122809059076 1102 100 2.84929142241214 1103 1 6.525642375743278 1103 2 10.777462741372336 1103 3 7.780987815624549 1103 4 11.66389859239834 1103 5 10.287705515078144 1103 6 8.837223753830234 1103 7 7.8761802105190775 1103 8 7.935610998906871 1103 9 7.10123071435083 1103 10-11 7.427582107521101 1103 12-13 8.634057165022835 1103 14-15 8.35961129159173 1103 16-17 7.935562018586921 1103 18-19 7.205552675309452 1103 20-21 8.209169851586353 1103 22-23 6.839347444110203 1103 24-25 5.192188044524777 1103 26-27 5.92901668611103 1103 28-29 6.931845946630389 1103 30-31 6.0505390006855855 1103 32-33 7.894270915433356 1103 34-35 7.087799512698336 1103 36-37 4.069573726517959 1103 38-39 6.334178104867565 1103 40-41 6.671340559874487 1103 42-43 7.371910811772597 1103 44-45 5.657197163263401 1103 46-47 6.346750647298052 1103 48-49 5.633549206075415 1103 50-51 6.3484202695729905 1103 52-53 6.057363920322409 1103 54-55 4.762554731108747 1103 56-57 5.852061078647029 1103 58-59 5.350320693112263 1103 60-61 4.88219790503425 1103 62-63 2.821142816951653 1103 64-65 4.984743804369195 1103 66-67 4.174288019523093 1103 68-69 5.15239488749479 1103 70-71 3.94144691225177 1103 72-73 4.456765156663202 1103 74-75 3.2532739551540857 1103 76-77 3.632681315236116 1103 78-79 2.8818306077846323 1103 80-81 3.975198266131155 1103 82-83 3.2926620610421367 1103 84-85 3.194709172851777 1103 86-87 2.707462252534093 1103 88-89 2.8257132151715645 1103 90-91 2.4651464759125403 1103 92-93 2.248491667996138 1103 94-95 2.6953460029912506 1103 96-97 2.8668262728990257 1103 98-99 2.6287083394721975 1103 100 2.48727413122122 1104 1 6.266841870692772 1104 2 9.529493465278062 1104 3 7.268100610237344 1104 4 12.020325360075113 1104 5 9.444496339993968 1104 6 8.122267945749424 1104 7 3.74044157415544 1104 8 6.407936335607204 1104 9 6.453191993812116 1104 10-11 6.978838420652419 1104 12-13 7.306669749197919 1104 14-15 7.017720508763443 1104 16-17 7.00638483003474 1104 18-19 6.747804358811141 1104 20-21 7.178370188286685 1104 22-23 6.259441088891343 1104 24-25 5.9000805108547425 1104 26-27 8.057108016077361 1104 28-29 7.032590896125342 1104 30-31 6.131817409776492 1104 32-33 7.479846462571398 1104 34-35 7.780850859499687 1104 36-37 6.434404324161058 1104 38-39 7.036004704194166 1104 40-41 6.943799524520951 1104 42-43 7.096745618169905 1104 44-45 6.600870353499094 1104 46-47 6.563809780294683 1104 48-49 5.3089816555703635 1104 50-51 5.491444259471976 1104 52-53 5.685856134127125 1104 54-55 4.6547674836339965 1104 56-57 5.909971432182385 1104 58-59 3.7400081931122635 1104 60-61 4.266309857896207 1104 62-63 3.6793583051671455 1104 64-65 2.8398700669954557 1104 66-67 3.1554875144725862 1104 68-69 4.054127842040241 1104 70-71 2.5972939240362827 1104 72-73 2.3556056448786897 1104 74-75 3.248638012393137 1104 76-77 3.81935008627989 1104 78-79 4.244029681858706 1104 80-81 3.050930589363478 1104 82-83 2.8639688792239575 1104 84-85 2.938633836151439 1104 86-87 0.7880230690324126 1104 88-89 0.4163592589426095 1104 90-91 2.236321770525336 1104 92-93 2.366584050151019 1104 94-95 2.3200261376713875 1104 96-97 2.699056912629665 1104 98-99 2.405837969101828 1104 100 0.9117122625343512 1105 1 5.523308990424084 1105 2 -2.69719350212943 1105 3 0.4297157216341141 1105 4 8.35420296960774 1105 5 8.415107131838386 1105 6 7.8062455099125785 1105 7 5.07324677841261 1105 8 5.834817304791667 1105 9 4.672366085529873 1105 10-11 4.761854955306504 1105 12-13 4.816849000643547 1105 14-15 5.433613098100356 1105 16-17 5.754733753874927 1105 18-19 5.86717229477296 1105 20-21 6.71401207450171 1105 22-23 1.9464337016481537 1105 24-25 3.814189565111178 1105 26-27 6.583153850802237 1105 28-29 5.672264692731453 1105 30-31 5.024744851694106 1105 32-33 6.767653310421917 1105 34-35 5.821149221577521 1105 36-37 5.848847938932188 1105 38-39 3.823816803141632 1105 40-41 5.05596216779713 1105 42-43 5.313930397445294 1105 44-45 5.8234738709989 1105 46-47 5.147204288907531 1105 48-49 4.734857105326817 1105 50-51 5.598115502469639 1105 52-53 5.328420987800055 1105 54-55 5.029529608941978 1105 56-57 4.914631649451774 1105 58-59 1.7350240487995165 1105 60-61 4.265484451093723 1105 62-63 -0.8029762826532512 1105 64-65 -0.3645580255330714 1105 66-67 0.8967422719664278 1105 68-69 2.7453962261306764 1105 70-71 2.917660067273605 1105 72-73 2.7053567297553194 1105 74-75 2.5511735964516475 1105 76-77 1.9213113578645213 1105 78-79 3.2110244370630703 1105 80-81 1.5947444295746962 1105 82-83 1.0304902217824186 1105 84-85 1.2992996542408406 1105 86-87 -0.19588921086786115 1105 88-89 1.5284613176876825 1105 90-91 1.458188493483764 1105 92-93 1.6165512307950571 1105 94-95 0.9180282134300128 1105 96-97 1.9658037110358535 1105 98-99 1.967100416827158 1105 100 2.037560183415021 1106 1 6.123505223679654 1106 2 10.306907500045039 1106 3 6.809494945749329 1106 4 11.611057340512971 1106 5 9.904914054088152 1106 6 9.213694084969685 1106 7 6.509389537956345 1106 8 7.313908767314931 1106 9 6.821500836385663 1106 10-11 5.607826262875552 1106 12-13 7.822830603594074 1106 14-15 7.634949072683181 1106 16-17 7.691174849383579 1106 18-19 7.067559222683645 1106 20-21 8.085666663597863 1106 22-23 6.205980388371827 1106 24-25 4.996160560978911 1106 26-27 7.623489211135027 1106 28-29 6.176481727516169 1106 30-31 5.411439136089395 1106 32-33 7.262133891917653 1106 34-35 6.735717207748383 1106 36-37 5.858065902234401 1106 38-39 7.042807269820255 1106 40-41 6.551294154074405 1106 42-43 6.0311999846095645 1106 44-45 4.435551535606745 1106 46-47 5.870738418105674 1106 48-49 5.837723542768135 1106 50-51 6.528150404634005 1106 52-53 5.507586351077929 1106 54-55 5.289514272792552 1106 56-57 4.912985166813765 1106 58-59 5.038740710594784 1106 60-61 4.811901045031508 1106 62-63 4.9095243129066795 1106 64-65 5.082300022660707 1106 66-67 2.9541114803759676 1106 68-69 3.440401494858012 1106 70-71 2.9492914635338217 1106 72-73 2.9482425150890883 1106 74-75 2.9364669998544812 1106 76-77 3.416728946526394 1106 78-79 4.230835903959047 1106 80-81 3.794491766748184 1106 82-83 2.2691776410503657 1106 84-85 2.6536764188410835 1106 86-87 3.3689306554547045 1106 88-89 3.4561914991424985 1106 90-91 3.263970048173615 1106 92-93 3.0543078187571417 1106 94-95 1.5855028314569068 1106 96-97 1.5256638568836678 1106 98-99 2.072382753293674 1106 100 2.9820222629914106 1107 1 5.895819285928653 1107 2 10.14407703612574 1107 3 5.9620643256457555 1107 4 11.021177561548079 1107 5 9.201772354465241 1107 6 7.669767489721881 1107 7 3.4045473611449744 1107 8 5.942785865518811 1107 9 5.4164157475268375 1107 10-11 4.451063493046785 1107 12-13 6.1211026409445815 1107 14-15 5.955225496564712 1107 16-17 6.704013391799869 1107 18-19 5.957421219669538 1107 20-21 5.454145482614457 1107 22-23 5.488864746080239 1107 24-25 4.4217520323146395 1107 26-27 7.05474189816384 1107 28-29 6.252323492974185 1107 30-31 4.969304156475996 1107 32-33 4.8067251060414655 1107 34-35 6.335902409613308 1107 36-37 6.314965960083871 1107 38-39 6.228438827215193 1107 40-41 7.417461083679125 1107 42-43 5.98018474039289 1107 44-45 4.041767521188024 1107 46-47 3.505809727091467 1107 48-49 3.7793833303315196 1107 50-51 4.483946169087318 1107 52-53 3.7240415530264244 1107 54-55 4.960206182131387 1107 56-57 5.011986218875503 1107 58-59 2.8520605735760434 1107 60-61 2.841700949018108 1107 62-63 0.49320477399385965 1107 64-65 1.9862406577791418 1107 66-67 3.9764090606339764 1107 68-69 4.790372198699384 1107 70-71 3.995827320453426 1107 72-73 2.346789824903162 1107 74-75 2.356455987098812 1107 76-77 2.959541266327161 1107 78-79 3.8730132724675777 1107 80-81 2.0901290069478975 1107 82-83 1.5530592752838785 1107 84-85 2.103109670491836 1107 86-87 2.058412963082013 1107 88-89 2.346716490019368 1107 90-91 1.3594017045533526 1107 92-93 2.1014771876965277 1107 94-95 2.1074614862664447 1107 96-97 2.072237552740326 1107 98-99 1.5898754849677843 1107 100 -0.09564830704405125 1108 1 4.43520103802398 1108 2 -3.159187477925908 1108 3 0.12692805685261987 1108 4 6.595507693275536 1108 5 7.041318453674634 1108 6 6.70543099067234 1108 7 4.049296986834868 1108 8 4.9515813936437105 1108 9 5.059969749438551 1108 10-11 3.484306339977241 1108 12-13 2.672467252742134 1108 14-15 4.22033360518822 1108 16-17 4.961727040516749 1108 18-19 4.219456184081384 1108 20-21 4.811345016060031 1108 22-23 4.254976555952304 1108 24-25 3.162163373472147 1108 26-27 4.8366880128654195 1108 28-29 6.142272207595301 1108 30-31 4.638981983141722 1108 32-33 4.750519544819316 1108 34-35 3.1434793372597376 1108 36-37 1.3634196695004164 1108 38-39 2.7108351772359853 1108 40-41 5.553166217769224 1108 42-43 3.437894912649792 1108 44-45 4.085936198351121 1108 46-47 4.833636612210327 1108 48-49 4.974309896864888 1108 50-51 2.2971524515905344 1108 52-53 0.43292038962065504 1108 54-55 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20-21 -23.314580148413654 2212 22-23 -18.30735171769738 2212 24-25 -24.784999455475223 2212 26-27 -16.91383256073056 2212 28-29 -17.745560515954644 2212 30-31 -6.0560332174874 2212 32-33 -16.915809563186667 2212 34-35 -13.434815890605844 2212 36-37 -17.282286392529656 2212 38-39 -23.313321895132436 2212 40-41 -9.945774053828135 2212 42-43 -13.675364759170066 2212 44-45 -16.758613393102 2212 46-47 -16.693943898377363 2212 48-49 -17.261960086927502 2212 50-51 -8.708944283391055 2212 52-53 -2.1780639829817687 2212 54-55 -23.13942443555792 2212 56-57 -23.047730588019636 2212 58-59 -12.203960465779865 2212 60-61 -6.0612180327694425 2212 62-63 -3.004890953699462 2212 64-65 -16.91315949980962 2212 66-67 -17.368829084461357 2212 68-69 -14.433489769939614 2212 70-71 -9.446256865687975 2212 72-73 -17.20898860912883 2212 74-75 -6.125261637654468 2212 76-77 -10.811721624511211 2212 78-79 -4.541050223119157 2212 80-81 -2.085712812586049 2212 82-83 -2.4930248923106326 2212 84-85 -4.835817430646763 2212 86-87 -4.487385597320134 2212 88-89 -2.6872089179674035 2212 90-91 -3.6317847072750205 2212 92-93 -4.95070202733914 2212 94-95 -3.243134317708453 2212 96-97 -4.924517264515941 2212 98-99 -3.564899532247921 2212 100 -3.427630509206381 2213 1 -20.83227429092339 2213 2 -16.73607892529433 2213 3 -19.693178851042116 2213 4 -18.70568474093499 2213 5 -20.859586151588523 2213 6 -21.382151246169766 2213 7 -10.230613595138335 2213 8 -10.120585635456672 2213 9 -4.420373965237083 2213 10-11 -17.387178347341827 2213 12-13 -15.965867479206253 2213 14-15 -23.209867875074938 2213 16-17 -17.641113706050756 2213 18-19 -18.272697499302033 2213 20-21 -17.355963071409988 2213 22-23 -19.4343185786766 2213 24-25 -7.5251775591944465 2213 26-27 -22.877024980555635 2213 28-29 -23.771070720036278 2213 30-31 -19.14922325485833 2213 32-33 -22.983958251233314 2213 34-35 -17.484243223463704 2213 36-37 -23.37157210681537 2213 38-39 -11.289749344058576 2213 40-41 -16.833117762545626 2213 42-43 -22.886735021560735 2213 44-45 -16.83863960773363 2213 46-47 -16.65930820768711 2213 48-49 -6.151759365166072 2213 50-51 -23.008871397093678 2213 52-53 -17.50944521840642 2213 54-55 -17.31517089967526 2213 56-57 -17.24600193427422 2213 58-59 -17.802995342770398 2213 60-61 -18.94673958405253 2213 62-63 -17.30187818007995 2213 64-65 -17.353850136612124 2213 66-67 -8.53531604892461 2213 68-69 -22.431667612505212 2213 70-71 -17.138309777550916 2213 72-73 -9.319100370663497 2213 74-75 -4.43709873124368 2213 76-77 -3.2498482595849083 2213 78-79 -4.7983524068827315 2213 80-81 -3.2172336353879665 2213 82-83 -3.334942378454979 2213 84-85 -3.1676988287197254 2213 86-87 -3.231575692725201 2213 88-89 -2.834822951233182 2213 90-91 -2.7848930845030324 2213 92-93 -3.7183602505476046 2213 94-95 -3.6598118021423254 2213 96-97 -3.3317598105652664 2213 98-99 -3.9273013514654664 2213 100 -3.200600956084525 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 612.0 3 635.0 4 3312.0 5 10090.0 6 15118.0 7 19047.0 8 18514.0 9 19328.0 10 23737.0 11 29527.0 12 37897.0 13 50628.0 14 43638.0 15 46826.0 16 50974.0 17 52526.0 18 51907.0 19 51330.0 20 50043.0 21 48686.0 22 49542.0 23 53215.0 24 57255.0 25 62302.0 26 67829.0 27 72625.0 28 77679.0 29 80643.0 30 83972.0 31 91052.0 32 101759.0 33 122351.0 34 146005.0 35 169868.0 36 182775.0 37 154459.0 38 46515.0 39 100.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.469543834144 21.604999729347263 13.62688200313957 24.298574433369165 2 14.498532418539902 21.97043757434868 40.3486904862707 23.18233952084072 3 17.26575367137383 27.75906972114858 30.83816569940353 24.13701090807406 4 11.658404190796452 18.219344731412942 40.113364135156466 30.00888694263414 5 12.436933442989256 38.05443420288853 36.79417047971755 12.714461874404662 6 28.9521367251298 39.10827100930368 18.9807527860668 12.958839479499723 7 24.60576330574518 32.31612396544433 25.88292152479913 17.195191204011355 8 23.769658801121146 36.69335064851747 22.62222004054358 16.914770509817796 9 24.157528613207884 17.350357934415847 22.874735132279294 35.61737832009697 10-11 22.591569023594744 27.995626112329 29.83696025786937 19.57584460620689 12-13 23.363167797443303 25.751439157172477 29.83472745447987 21.050665590904345 14-15 20.979875382471388 27.094978635349765 27.16216307005993 24.762982912118918 16-17 20.501179223922126 30.821278440550326 27.996599391126775 20.68094294440077 18-19 20.390449333190148 29.87168133901543 29.540627813496595 20.197241514297833 20-21 21.86438568229526 29.230494066918915 28.448286905166174 20.456833345619646 22-23 20.757930498533657 29.360266785796124 29.058525650238458 20.82327706543176 24-25 21.142515110615573 29.076908632122528 28.450325685112375 21.330250572149524 26-27 21.120601556321112 29.03688684433728 28.640919534782228 21.20159206455938 28-29 20.818257339873767 28.92319694444029 29.192563404693235 21.065982310992712 30-31 21.62352406427437 29.392588725955093 28.323939032534728 20.65994817723581 32-33 20.497265456768286 29.22891462312196 29.1485807892458 21.125239130863964 34-35 20.68742470040073 28.627371405832886 28.968565883570797 21.716638010195588 36-37 21.368971708411124 29.289495189296556 28.392873120306355 20.94865998198596 38-39 21.03310741015543 28.91979399370265 28.73853252860099 21.308566067540927 40-41 21.308234625285014 28.964958166251037 28.278733308298875 21.448073900165078 42-43 20.882334539376572 29.127740271146813 28.92405543596096 21.065869753515656 44-45 21.412026322171247 28.488888328300334 28.814928748268954 21.28415660125947 46-47 21.24760333315591 29.12250944768731 28.88199705480546 20.74789016435132 48-49 21.056531627250124 28.834763068343722 28.969192705743076 21.139512598663078 50-51 21.415599127461395 28.84139120480706 28.60520961354311 21.13780005418844 52-53 20.732623481213626 29.029921829921086 28.846079930227663 21.39137475863762 54-55 21.29044886980006 28.919313111171626 28.406925833211773 21.383312185816543 56-57 21.426111869943483 28.85416260010178 28.431419073157493 21.288306456797244 58-59 21.014340869316843 28.38725824033233 28.981025738321254 21.617375152029574 60-61 21.605266577559963 28.855791007648325 28.346536905027104 21.19240550976461 62-63 20.867170822572298 29.074149325922527 28.853327477216624 21.205352374288555 64-65 20.93017453277092 28.47458306915296 28.861835160307255 21.73340723776887 66-67 21.193217720584624 28.760745141190842 28.56521585121857 21.480821287005963 68-69 21.595574075805693 28.663493038852923 28.48702414624092 21.253908739100467 70-71 21.716703175853816 28.795868449962843 28.13182691150095 21.355601462682387 72-73 21.23901129840015 28.969625345032906 28.74778213323166 21.043581223335284 74-75 21.623256754025466 28.350962294235217 28.37613689954861 21.64964405219071 76-77 21.537439642079924 28.959327571396564 28.38973118842479 21.11350159809872 78-79 21.49738212541376 28.652558620975576 28.676119605525685 21.17393964808498 80-81 21.718245532935615 28.592169488193992 28.300317945059383 21.38926703381101 82-83 21.074648464844014 28.926956231732547 28.74725265846603 21.251142644957405 84-85 21.333012738679827 28.470382665173666 28.285935577150372 21.910669018996135 86-87 21.63541219507121 28.77573038513565 28.300979767342444 21.287877652450693 88-89 21.372370080084842 28.271938840228795 28.74990643247069 21.605784647215675 90-91 21.764590576193026 28.3973160823411 28.270661852630113 21.567431488835766 92-93 21.16846235314621 28.761423202563268 28.702592312882913 21.367522131407608 94-95 21.18412191405824 28.2302515516634 28.63445782317633 21.951168711102035 96-97 21.48745005586487 28.54837109502985 28.47547466104278 21.4887041880625 98-99 21.386796221225897 28.308939286965064 28.526857693998963 21.777406797810077 100 21.5922270021541 28.199874277160735 28.293093380951827 21.914805339733334 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 254.0 1 317.0 2 337.0 3 284.0 4 296.0 5 397.5 6 607.5 7 901.5 8 1300.0 9 1841.5 10 2520.0 11 3271.5 12 4003.5 13 4616.5 14 5317.5 15 6124.0 16 6828.5 17 7640.0 18 8434.0 19 9216.5 20 10189.5 21 11299.0 22 12611.5 23 14325.5 24 16603.5 25 19590.0 26 23655.0 27 28655.0 28 34449.5 29 41050.5 30 47857.5 31 54361.0 32 60934.5 33 67246.0 34 72578.5 35 77484.5 36 81702.0 37 85201.0 38 87828.5 39 88668.0 40 88442.5 41 87320.0 42 84930.0 43 81481.0 44 77161.0 45 73210.5 46 69576.0 47 65847.0 48 62812.5 49 60795.5 50 59954.5 51 56404.0 52 49494.5 53 43708.0 54 39438.5 55 35601.0 56 32133.5 57 28795.0 58 25409.5 59 22025.5 60 18820.5 61 15902.5 62 13199.5 63 10804.5 64 8709.5 65 6966.5 66 5592.5 67 4458.5 68 3503.5 69 2738.0 70 2096.5 71 1587.5 72 1262.5 73 984.0 74 695.5 75 514.0 76 374.0 77 272.5 78 196.5 79 126.0 80 84.0 81 58.0 82 46.5 83 37.0 84 21.0 85 18.0 86 14.0 87 7.5 88 5.5 89 3.5 90 2.0 91 1.0 92 0.0 93 2.0 94 2.0 95 0.0 96 0.0 97 0.0 98 0.5 99 0.5 100 1.0 >>END_MODULE >>Per base N content fail #Base N-Count 1 7.808553232804042 2 24.75082664199996 3 5.989161087028103 4 23.891279366899834 5 12.340257922106508 6 9.752838386309266 7 7.806191716782047 8 8.322428030571384 9 4.869757935696365 10-11 6.666470616279935 12-13 9.761994830507756 14-15 7.151606209628659 16-17 7.757513296894696 18-19 6.438896218499939 20-21 7.8252397941292715 22-23 5.597127148923884 24-25 5.088220446183946 26-27 9.747937126640975 28-29 6.218695988675417 30-31 4.227536969978893 32-33 8.594202879178157 34-35 7.00619474665166 36-37 6.627104589762338 38-39 5.987802101392805 40-41 8.457769632775348 42-43 6.351341898061818 44-45 6.387432991982876 46-47 5.823966357753637 48-49 3.912207745683438 50-51 6.426977246124775 52-53 4.3626335091994415 54-55 4.8533387158075865 56-57 4.6978796705105905 58-59 3.5948289037085157 60-61 3.9923433412600065 62-63 3.0155891247285926 64-65 4.4606141456592 66-67 2.5136110208833764 68-69 4.936927471604998 70-71 2.477475370056244 72-73 1.4837449723101108 74-75 0.7962764684730927 76-77 1.0566001922184927 78-79 1.6611928507553064 80-81 2.0699801855438684 82-83 0.7345428751056555 84-85 1.1374264198392297 86-87 0.4075842983245267 88-89 0.5931415336754412 90-91 0.21204631601272725 92-93 0.29189229169980585 94-95 0.5256600994242802 96-97 0.5213826364410439 98-99 0.4455022253946739 100 0.34108311600702396 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 2244321.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 96.8853309708689 #Duplication Level Percentage of deduplicated Percentage of total 1 98.29775499998196 95.23610526866636 2 1.3819109441423492 2.6777379839099487 3 0.15682149451869962 0.45581107199371557 4 0.06247226669375157 0.24210584940498042 5 0.03182760164677976 0.1541813859778615 6 0.018140708637216795 0.10545411362177098 7 0.011041990051750527 0.07488648025186241 8 0.008078823886761342 0.06261756208993874 9 0.007473618606142825 0.06516756109855724 >10 0.022030161873923836 0.3590676192551946 >50 8.286406873539049E-4 0.05398138065903945 >100 0.0013878500498696574 0.27101119902655224 >500 1.3853953411322584E-4 0.08460616777057461 >1k 9.235968940877697E-5 0.15726635627363544 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 4.45569060753787E-5 0.0 8 0.0 0.0 0.0 4.45569060753787E-5 0.0 9 0.0 0.0 0.0 4.45569060753787E-5 0.0 10-11 0.0 0.0 0.0 1.782276243015148E-4 0.0 12-13 0.0 0.0 0.0 2.0050607733920414E-4 0.0 14-15 0.0 0.0 0.0 2.673414364522722E-4 0.0 16-17 2.227845303768935E-5 0.0 0.0 2.673414364522722E-4 0.0 18-19 8.91138121507574E-5 0.0 0.0 2.673414364522722E-4 0.0 20-21 8.91138121507574E-5 0.0 0.0 2.896198894899616E-4 0.0 22-23 8.91138121507574E-5 2.227845303768935E-5 0.0 3.7873370164071896E-4 0.0 24-25 1.336707182261361E-4 4.45569060753787E-5 0.0 5.569613259422338E-4 0.0 26-27 1.336707182261361E-4 4.45569060753787E-5 0.0 5.792397789799232E-4 0.0 28-29 1.336707182261361E-4 4.45569060753787E-5 0.0 9.134165745452634E-4 0.0 30-31 1.5594917126382545E-4 4.45569060753787E-5 0.0 0.003118983425276509 0.0 32-33 1.782276243015148E-4 6.683535911306805E-5 0.0 0.008488090607359642 0.0 34-35 2.227845303768935E-4 8.91138121507574E-5 0.0 0.017087573479907733 0.0 36-37 2.227845303768935E-4 8.91138121507574E-5 0.0 0.029853127070503728 0.0 38-39 2.227845303768935E-4 8.91138121507574E-5 0.0 0.04734171270508987 0.0 40-41 2.227845303768935E-4 8.91138121507574E-5 0.0 0.07064497458251293 0.0 42-43 2.227845303768935E-4 8.91138121507574E-5 0.0 0.09221051712299622 0.0 44-45 2.227845303768935E-4 1.336707182261361E-4 0.0 0.11918972375163801 0.0 46-47 2.227845303768935E-4 1.336707182261361E-4 0.0 0.15193904971704136 0.0 48-49 2.227845303768935E-4 1.782276243015148E-4 0.0 0.18798558673202273 0.0 50-51 2.227845303768935E-4 3.564552486030296E-4 0.0 0.2234083270619488 0.0 52-53 2.227845303768935E-4 3.564552486030296E-4 0.0 0.2621728353475283 0.0 54-55 2.227845303768935E-4 3.564552486030296E-4 0.0 0.3079327778869422 0.0 56-57 2.227845303768935E-4 3.564552486030296E-4 0.0 0.36195802650333886 0.0 58-59 2.227845303768935E-4 3.7873370164071896E-4 0.0 0.4225776972188916 0.0 60-61 2.4506298341458283E-4 4.010121546784083E-4 0.0 0.4858930607520047 0.0 62-63 2.673414364522722E-4 4.010121546784083E-4 0.0 0.5500104485944747 0.0 64-65 2.673414364522722E-4 4.010121546784083E-4 0.0 0.62593541654692 0.0 66-67 2.673414364522722E-4 4.010121546784083E-4 0.0 0.716363657426901 0.0 68-69 2.673414364522722E-4 4.010121546784083E-4 0.0 0.8191787181958374 0.0 70-71 3.7873370164071896E-4 4.010121546784083E-4 0.0 0.9430691955384278 0.0 72-73 4.45569060753787E-4 4.010121546784083E-4 0.0 1.0682963800632796 0.0 74-75 4.45569060753787E-4 4.010121546784083E-4 0.0 1.1958850806101267 0.0 76-77 4.45569060753787E-4 4.010121546784083E-4 0.0 1.3383112308800746 0.0 78-79 4.45569060753787E-4 4.010121546784083E-4 0.0 1.5031940618120134 0.0 80-81 4.45569060753787E-4 4.010121546784083E-4 0.0 1.6932292662235038 0.0 82-83 4.45569060753787E-4 4.010121546784083E-4 0.0 1.8997728043359217 0.0 84-85 4.45569060753787E-4 4.45569060753787E-4 0.0 2.108945199906787 0.0 86-87 4.45569060753787E-4 4.45569060753787E-4 0.0 2.335828965642615 2.227845303768935E-5 88 4.45569060753787E-4 4.45569060753787E-4 0.0 2.529495557899249 4.45569060753787E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTAGTAC 495 0.0 21.577217 3 TATAGCG 185 0.002306628 16.137974 7 CATGGGT 1585 0.0 15.60524 4 GTATTAG 960 0.0 15.5571785 1 GTACATG 8545 0.0 15.438837 1 GTATAGA 970 0.0 15.396794 1 TACATGG 8695 0.0 14.883287 2 GTATAGG 690 9.276846E-11 14.429846 1 ACATGGG 9000 0.0 14.240963 3 TGTACTG 1055 0.0 14.238126 5 TCTATAC 795 0.0 14.141971 3 GTATAAC 580 2.9373041E-8 13.947814 1 ACAGCGT 330 3.8063212E-5 13.667228 8 GTACTGT 900 0.0 13.353611 6 GTATATA 1865 0.0 13.34664 1 GTATTAA 1495 0.0 13.319859 1 GTACTGA 720 2.7157512E-9 12.964314 1 GTACAAG 920 3.6379788E-12 12.851582 1 GTATTAC 735 3.6925485E-9 12.699737 1 GTATTAT 1245 0.0 12.495725 1 >>END_MODULE