FastQCFastQC Report
Wed 25 May 2016
SRR1295087_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295087_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2244321
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT57170.25473183203294003No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA42010.18718356242266593No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGTACT29550.016.60480512-13
CCTATAC6000.015.6720323
GGACCGT1800.002826978215.6535126
GTATAGG5305.0931703E-1114.983891
TATGCCG5651.8189894E-1214.96454994
ATACGAT2206.2202313E-414.941996
CATGGGT17750.014.8342394
ACTCCGC2257.3830923E-414.6079918
CTAGGAC7750.014.5598223
GTATCAA103850.014.48161
CTATACT9500.014.3532434
GTCTAGC5206.148184E-1014.3175371
AGTACTT35500.013.82174512-13
GTACTTT36550.013.74538314-15
CAACGCA109100.013.661875
AACGCAG108950.013.5773936
ATCAACG110900.013.5664113
TCAACGC110750.013.543244
GTACATG121000.013.5365811
TTGGCGT2802.4898967E-413.4340694
Produced by FastQC (version 0.11.5)