##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1295087_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2244321 Sequences flagged as poor quality 0 Sequence length 100 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.11209804658068 33.0 33.0 33.0 27.0 33.0 2 29.866040553022497 33.0 33.0 33.0 22.0 33.0 3 30.671998346047648 33.0 33.0 33.0 27.0 33.0 4 34.919711128666535 37.0 37.0 37.0 33.0 37.0 5 35.213007854045834 37.0 37.0 37.0 33.0 37.0 6 34.86835706656935 37.0 37.0 37.0 33.0 37.0 7 34.76008111139182 37.0 37.0 37.0 27.0 37.0 8 34.74256801945889 37.0 37.0 37.0 27.0 37.0 9 34.728909099901486 37.0 37.0 37.0 27.0 37.0 10-11 34.690886464102064 37.0 37.0 37.0 27.0 37.0 12-13 34.58987217069216 37.0 37.0 37.0 27.0 37.0 14-15 35.964914332664534 37.0 37.0 40.0 27.0 40.0 16-17 35.95759430134994 37.0 37.0 40.0 27.0 40.0 18-19 35.98779875962485 37.0 37.0 40.0 27.0 40.0 20-21 35.991480051204796 37.0 37.0 40.0 27.0 40.0 22-23 35.95680453018976 37.0 37.0 40.0 27.0 40.0 24-25 35.918867443649994 37.0 37.0 40.0 27.0 40.0 26-27 35.88991592557393 37.0 37.0 40.0 27.0 40.0 28-29 35.829862127565534 37.0 37.0 40.0 27.0 40.0 30-31 35.73491091514984 37.0 37.0 40.0 27.0 40.0 32-33 35.691943131129634 37.0 37.0 40.0 27.0 40.0 34-35 35.58014628923403 37.0 37.0 40.0 27.0 40.0 36-37 35.56882816673729 37.0 37.0 40.0 27.0 40.0 38-39 35.50431355407716 37.0 37.0 40.0 27.0 40.0 40-41 35.45020075114032 37.0 37.0 40.0 27.0 40.0 42-43 35.36320183253643 37.0 35.0 40.0 27.0 40.0 44-45 35.26243839450774 37.0 33.0 40.0 27.0 40.0 46-47 35.14621259614823 37.0 33.0 40.0 27.0 40.0 48-49 35.041583178163904 37.0 33.0 40.0 24.5 40.0 50-51 34.94113698530647 37.0 33.0 40.0 22.0 40.0 52-53 34.85164265717783 37.0 33.0 40.0 22.0 40.0 54-55 34.74271572560254 37.0 33.0 40.0 22.0 40.0 56-57 34.61504258971867 37.0 33.0 40.0 22.0 40.0 58-59 34.490549034652354 37.0 33.0 40.0 22.0 40.0 60-61 34.334588278592946 37.0 33.0 40.0 22.0 40.0 62-63 34.15450374523074 37.0 33.0 40.0 22.0 40.0 64-65 33.99365799277376 37.0 33.0 40.0 22.0 40.0 66-67 33.77906770020866 37.0 33.0 40.0 22.0 40.0 68-69 33.57160250249407 37.0 33.0 37.0 22.0 40.0 70-71 33.34512420460353 37.0 33.0 37.0 22.0 40.0 72-73 33.12995400390586 37.0 33.0 37.0 22.0 40.0 74-75 32.89959613620333 37.0 33.0 37.0 22.0 40.0 76-77 32.66309476229114 37.0 33.0 37.0 22.0 38.5 78-79 32.43358280745045 37.0 33.0 37.0 22.0 37.0 80-81 32.21848657121686 37.0 33.0 37.0 22.0 37.0 82-83 32.01571343849654 35.0 33.0 37.0 22.0 37.0 84-85 31.83409414250457 33.0 33.0 37.0 22.0 37.0 86-87 31.8468019949018 33.0 33.0 37.0 22.0 37.0 88-89 31.84797428710064 33.0 33.0 37.0 22.0 37.0 90-91 31.844154423542797 33.0 33.0 37.0 22.0 37.0 92-93 31.800291491279545 33.0 33.0 37.0 22.0 37.0 94-95 31.819583517687533 33.0 33.0 37.0 22.0 37.0 96-97 31.77173096005429 33.0 33.0 37.0 22.0 37.0 98-99 31.68966916942808 33.0 33.0 37.0 22.0 37.0 100 31.153675432346798 33.0 27.0 37.0 15.0 37.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.5966024900547566 1101 2 4.100370141477953 1101 3 2.374226977868709 1101 4 1.2137830974587445 1101 5 0.5884684441488659 1101 6 0.7227883682160012 1101 7 0.6065360893308664 1101 8 0.5788635437112433 1101 9 0.6888905748787124 1101 10-11 0.7209196666046438 1101 12-13 0.7488692159724124 1101 14-15 1.1136837216195659 1101 16-17 1.1228908402901325 1101 18-19 1.1116645537244523 1101 20-21 1.0982790646808382 1101 22-23 0.9934000333055408 1101 24-25 1.032066406996968 1101 26-27 1.063479516775054 1101 28-29 0.9435342770763171 1101 30-31 0.7891483124226255 1101 32-33 1.0432135226479886 1101 34-35 1.2131870778819263 1101 36-37 1.1242255616015342 1101 38-39 1.1210185091812832 1101 40-41 1.1131489375968755 1101 42-43 1.1254006556868816 1101 44-45 1.1120496361493366 1101 46-47 1.1884143339518616 1101 48-49 1.085741633066867 1101 50-51 1.0848297441301256 1101 52-53 1.1885426186664532 1101 54-55 1.209691976528525 1101 56-57 1.1502119486582671 1101 58-59 1.1200838626903789 1101 60-61 1.0703893845629224 1101 62-63 1.0565339210662685 1101 64-65 1.0919954282289268 1101 66-67 1.1831869823055783 1101 68-69 1.0930923994862738 1101 70-71 1.1311813314285502 1101 72-73 1.0300796304120752 1101 74-75 1.1267764616161244 1101 76-77 1.132749899467342 1101 78-79 1.1171554798924248 1101 80-81 1.0908904615551833 1101 82-83 1.0847667905813871 1101 84-85 1.1018406145785882 1101 86-87 1.066242313979572 1101 88-89 0.9340596418679432 1101 90-91 1.0187606451509126 1101 92-93 1.074162319261827 1101 94-95 1.0542698774379105 1101 96-97 1.0469554125142402 1101 98-99 1.0400743115108462 1101 100 1.1426901793050845 1102 1 0.482001968458178 1102 2 3.9955024168261275 1102 3 2.2319326840278073 1102 4 1.1025078858098567 1102 5 0.4607100971669382 1102 6 0.60357469741421 1102 7 0.6565084435901198 1102 8 0.6205256835153818 1102 9 0.6649699956851407 1102 10-11 0.7276030159286364 1102 12-13 0.7259465443057138 1102 14-15 0.9905414354868327 1102 16-17 0.9677187914602072 1102 18-19 1.0352685628329255 1102 20-21 0.9276439547941209 1102 22-23 1.0113441975804207 1102 24-25 1.0428397447373001 1102 26-27 0.9515696517512211 1102 28-29 0.975234875234257 1102 30-31 0.902229869738143 1102 32-33 1.017880647356634 1102 34-35 1.0306886552703673 1102 36-37 0.9144533235700081 1102 38-39 0.9435751468199172 1102 40-41 1.012973295330724 1102 42-43 1.0420711266183034 1102 44-45 1.1501094772764375 1102 46-47 1.145761916403238 1102 48-49 1.0268594144638712 1102 50-51 0.9391700361650379 1102 52-53 1.046265560907763 1102 54-55 0.9660174274252853 1102 56-57 0.8074863107022026 1102 58-59 0.8340619810695813 1102 60-61 0.8752094560167976 1102 62-63 0.9853480939119947 1102 64-65 0.8451442330437047 1102 66-67 0.887904818926053 1102 68-69 0.8738593800664134 1102 70-71 0.8438518241229147 1102 72-73 0.7703373334318684 1102 74-75 0.7843510842883248 1102 76-77 0.7880847453253352 1102 78-79 0.8266255020311206 1102 80-81 0.8182236957650986 1102 82-83 0.7927419492001029 1102 84-85 0.7172290015312264 1102 86-87 0.8491045093132215 1102 88-89 0.7300637866887776 1102 90-91 0.7994114362594544 1102 92-93 0.9244290726062196 1102 94-95 0.827670696302711 1102 96-97 0.8432196075224745 1102 98-99 0.8627423779769252 1102 100 0.8990794313347799 1103 1 0.5868307865330529 1103 2 4.034641230749042 1103 3 2.2514991932659214 1103 4 1.119383015558661 1103 5 0.43349454979997404 1103 6 0.540710665513302 1103 7 0.47298264952742386 1103 8 0.47602980087749813 1103 9 0.5692079376960777 1103 10-11 0.5878312657037412 1103 12-13 0.6520745804277794 1103 14-15 0.9409712932821321 1103 16-17 0.8852339504457447 1103 18-19 0.9181121376720327 1103 20-21 0.8541940421583121 1103 22-23 0.7855555410860546 1103 24-25 0.7750178811984441 1103 26-27 0.8001123996579409 1103 28-29 0.7979635358805766 1103 30-31 0.8404697190565287 1103 32-33 0.9135403551623256 1103 34-35 0.9844091806665318 1103 36-37 0.8369891774901532 1103 38-39 0.8496983822361557 1103 40-41 0.8799497156476619 1103 42-43 0.9510366610103844 1103 44-45 0.9823219539216552 1103 46-47 1.0144424359174664 1103 48-49 0.9221318869571178 1103 50-51 0.9123079894208743 1103 52-53 0.9891962836951151 1103 54-55 0.9845783401648944 1103 56-57 0.8438301407139619 1103 58-59 0.9581576751391907 1103 60-61 1.0095179165039596 1103 62-63 1.0719994718443147 1103 64-65 1.0385551129136061 1103 66-67 0.927919783288381 1103 68-69 1.000734444940825 1103 70-71 0.8427389686111866 1103 72-73 0.8200811660386194 1103 74-75 0.854875355965028 1103 76-77 0.8134178970103392 1103 78-79 0.8148589504084001 1103 80-81 0.8223966552488733 1103 82-83 0.9420820035216018 1103 84-85 0.9478725556105267 1103 86-87 0.9763137206134758 1103 88-89 0.9807933642266669 1103 90-91 0.9396769023171672 1103 92-93 0.9900616845361903 1103 94-95 0.831509076864613 1103 96-97 0.8999040714004032 1103 98-99 1.00337821200225 1103 100 1.0280392784041865 1104 1 0.4067592377115048 1104 2 3.477099143206953 1104 3 1.9837740249157534 1104 4 0.855788739464387 1104 5 0.35805052623094724 1104 6 0.3489998069274449 1104 7 0.343289888584664 1104 8 0.23649276384045947 1104 9 0.3193847053728476 1104 10-11 0.39493459061957026 1104 12-13 0.38489444574764065 1104 14-15 0.4897444414302896 1104 16-17 0.6047846659339555 1104 18-19 0.5006415989514963 1104 20-21 0.45096697986875256 1104 22-23 0.4117445141500298 1104 24-25 0.4128335377641008 1104 26-27 0.4739297397252855 1104 28-29 0.4687526772947166 1104 30-31 0.42926285878716897 1104 32-33 0.5858499090343798 1104 34-35 0.6412031621480097 1104 36-37 0.4436074434834154 1104 38-39 0.4418848300475986 1104 40-41 0.514095338543278 1104 42-43 0.5041458781821078 1104 44-45 0.6485719539216532 1104 46-47 0.6494918887794157 1104 48-49 0.4831504054756408 1104 50-51 0.5067829473333347 1104 52-53 0.4778347517085848 1104 54-55 0.5675413031278467 1104 56-57 0.49220450940082827 1104 58-59 0.5285974899540093 1104 60-61 0.5216275545510953 1104 62-63 0.5314491772315222 1104 64-65 0.5363602475937483 1104 66-67 0.43471692133551443 1104 68-69 0.47577337591724955 1104 70-71 0.41430252079973684 1104 72-73 0.44175835459080304 1104 74-75 0.4411590259986937 1104 76-77 0.3789250518924945 1104 78-79 0.5059163999033487 1104 80-81 0.40821631013102433 1104 82-83 0.43249235705695455 1104 84-85 0.559286697024671 1104 86-87 0.5247491162363787 1104 88-89 0.4770202160785111 1104 90-91 0.3945085521488174 1104 92-93 0.5391841592836677 1104 94-95 0.4083682940363289 1104 96-97 0.4555259148347446 1104 98-99 0.41005224398878326 1104 100 0.4473869215018311 1105 1 0.38933780462413026 1105 2 3.715218273793333 1105 3 2.098045902560994 1105 4 0.9009618261221917 1105 5 0.35870678207279383 1105 6 0.38024370252723116 1105 7 0.2826091311244312 1105 8 0.23346169940110428 1105 9 0.31657589361206817 1105 10-11 0.33717409945295884 1105 12-13 0.3885377224531439 1105 14-15 0.4392712257005229 1105 16-17 0.4826305635669783 1105 18-19 0.47719354752855025 1105 20-21 0.3781842818131196 1105 22-23 0.364795637779757 1105 24-25 0.487345028214456 1105 26-27 0.4951771220534482 1105 28-29 0.41108775609267667 1105 30-31 0.3100731319282346 1105 32-33 0.49586717726671026 1105 34-35 0.6095098382876571 1105 36-37 0.46014432928885896 1105 38-39 0.5017153036335316 1105 40-41 0.4915844110105141 1105 42-43 0.6025738179037106 1105 44-45 0.5242542683321361 1105 46-47 0.5038078835157123 1105 48-49 0.4381710842954476 1105 50-51 0.2848148125649743 1105 52-53 0.28669147500308156 1105 54-55 0.3600303572162531 1105 56-57 0.31307758041036493 1105 58-59 0.4130899635567431 1105 60-61 0.3561513772026501 1105 62-63 0.29654050168419843 1105 64-65 0.25476807298847604 1105 66-67 0.2690782881750522 1105 68-69 0.26570435812443094 1105 70-71 0.32068773654630434 1105 72-73 0.28610021885624803 1105 74-75 0.38338882030272003 1105 76-77 0.3798770101315725 1105 78-79 0.40382345706258604 1105 80-81 0.4632855484699192 1105 82-83 0.426164063201373 1105 84-85 0.3852254356271594 1105 86-87 0.5025341536556951 1105 88-89 0.3008870687390335 1105 90-91 0.3453020322818148 1105 92-93 0.3823302174952268 1105 94-95 0.2724114864092151 1105 96-97 0.3224458550352516 1105 98-99 0.4162623225241866 1105 100 0.34773646077058373 1106 1 0.5035977205793998 1106 2 3.8240166592421154 1106 3 2.1266459949936056 1106 4 0.9299085194665224 1106 5 0.3511879715742765 1106 6 0.23597761669937256 1106 7 0.13248748292932078 1106 8 0.16144458217463153 1106 9 0.1974984217218534 1106 10-11 0.0870556503876827 1106 12-13 0.2151345351789118 1106 14-15 0.246278008625616 1106 16-17 0.23009841808595155 1106 18-19 0.3554743342989397 1106 20-21 0.16716268503504494 1106 22-23 0.049120346378799695 1106 24-25 0.07191302037299607 1106 26-27 0.16074605521806262 1106 28-29 0.18062250681160208 1106 30-31 0.17697994290131192 1106 32-33 0.1992572669270345 1106 34-35 0.4062466018238098 1106 36-37 0.23917149272394056 1106 38-39 0.1463769610103185 1106 40-41 0.19176189856278114 1106 42-43 0.21157223450551754 1106 44-45 0.2518602097834375 1106 46-47 0.17638363809764002 1106 48-49 0.15128347066752923 1106 50-51 0.13347644718859897 1106 52-53 0.1045139684613261 1106 54-55 0.10054622032394178 1106 56-57 0.15069695649621906 1106 58-59 0.11623622648980358 1106 60-61 0.19191974874034656 1106 62-63 0.2319520376608537 1106 64-65 0.10358605011366961 1106 66-67 0.15681821328975332 1106 68-69 0.06440050314731849 1106 70-71 0.24178025820394566 1106 72-73 0.09965995734534516 1106 74-75 0.06279202263169026 1106 76-77 0.25078514985553113 1106 78-79 0.16889659781001143 1106 80-81 0.14049050380228323 1106 82-83 0.14197548014302086 1106 84-85 0.09382895215515674 1106 86-87 0.08341029438285119 1106 88-89 0.10273675172151897 1106 90-91 0.13519263658290015 1106 92-93 0.25012844375736165 1106 94-95 0.15020692925731538 1106 96-97 0.06279344305815826 1106 98-99 0.12726871488172264 1106 100 0.15163433530806714 1107 1 0.4283089143119696 1107 2 3.450500386642055 1107 3 2.027137019040598 1107 4 0.9046102882859373 1107 5 0.2236359325894952 1107 6 0.2932917671618398 1107 7 0.08695633103778988 1107 8 0.12406249805241032 1107 9 0.1098685505970991 1107 10-11 0.1063242585555102 1107 12-13 0.14944196191762416 1107 14-15 0.18044684735559713 1107 16-17 0.10470319851332732 1107 18-19 0.14146171698662613 1107 20-21 0.16239892535826073 1107 22-23 0.1370910000777812 1107 24-25 0.1764573606429991 1107 26-27 0.17361802589776687 1107 28-29 0.048574598538756675 1107 30-31 0.09467236202117135 1107 32-33 0.14973770535727482 1107 34-35 0.32827587383980017 1107 36-37 0.09614913200140052 1107 38-39 0.09919343148712301 1107 40-41 0.13764270080395846 1107 42-43 0.14709840001442132 1107 44-45 0.3015898587031032 1107 46-47 0.24972223019204165 1107 48-49 0.16291836346030664 1107 50-51 0.10314703022393701 1107 52-53 0.05130197662897018 1107 54-55 0.12174136431155347 1107 56-57 0.1117713687391344 1107 58-59 0.16603017838838952 1107 60-61 0.30072804972100897 1107 62-63 0.27076364432533495 1107 64-65 0.20618110477352047 1107 66-67 0.1570526754150663 1107 68-69 0.09719664738285161 1107 70-71 0.1647573607720716 1107 72-73 0.05483845962915268 1107 74-75 0.1599087884843513 1107 76-77 0.12647936667310944 1107 78-79 0.1392225140926442 1107 80-81 0.22019021585493448 1107 82-83 0.1895429704140028 1107 84-85 0.2349257056207854 1107 86-87 0.225894552920753 1107 88-89 0.17617005722891932 1107 90-91 0.12194065154762157 1107 92-93 0.17820985643577458 1107 94-95 0.16723375451014277 1107 96-97 0.16413215215147758 1107 98-99 0.10732643434988276 1107 100 0.0954386388255557 1108 1 0.5334316637260343 1108 2 3.6480430947841285 1108 3 2.198999193265923 1108 4 1.0922037392428763 1108 5 0.3664742647122523 1108 6 0.27426603832032015 1108 7 0.16899416268532264 1108 8 0.12906708157925095 1108 9 0.005381183310113613 1108 10-11 0.16935401789672255 1108 12-13 0.17006800148040924 1108 14-15 0.046263234071609816 1108 16-17 0.08632221798959705 1108 18-19 0.23703894688256355 1108 20-21 0.2074040202284877 1108 22-23 0.08386283275272177 1108 24-25 0.20504118163703566 1108 26-27 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2202 82-83 1.220920809591977 2202 84-85 1.21914169340263 2202 86-87 1.2112256699822694 2202 88-89 1.1854272280461622 2202 90-91 1.256990560657215 2202 92-93 1.277839854023167 2202 94-95 1.3318257737488395 2202 96-97 1.3194596213601137 2202 98-99 1.2843408933076539 2202 100 1.1081604844208428 2203 1 0.3644997282249989 2203 2 3.89078716940762 2203 3 2.177490725116275 2203 4 1.052307686943898 2203 5 0.5710322562374515 2203 6 0.6389599493016433 2203 7 0.6339217294060902 2203 8 0.5754804244029472 2203 9 0.73814204622321 2203 10-11 0.6410838145746567 2203 12-13 0.7785419717321318 2203 14-15 0.9504878504105534 2203 16-17 0.9541957595121389 2203 18-19 1.0252633004627327 2203 20-21 0.9851009978374492 2203 22-23 1.0096318386930605 2203 24-25 1.0159409516399691 2203 26-27 0.9279431209252138 2203 28-29 0.8702269753817546 2203 30-31 0.9297137997778009 2203 32-33 1.0103587955061073 2203 34-35 1.129006732907854 2203 36-37 0.9585081781642373 2203 38-39 0.9287043225799323 2203 40-41 0.5480079394427335 2203 42-43 0.7619257113979856 2203 44-45 0.96088868799648 2203 46-47 1.0483930805079567 2203 48-49 1.0489089344798685 2203 50-51 0.9745003343821139 2203 52-53 1.0575230868632985 2203 54-55 0.9548079491975798 2203 56-57 0.9649757633968079 2203 58-59 1.0605070600397681 2203 60-61 0.962551178216124 2203 62-63 1.0675748636542224 2203 64-65 1.028238926530058 2203 66-67 1.1029682329007855 2203 68-69 1.054118229066198 2203 70-71 1.0551435439397991 2203 72-73 1.0995871906425947 2203 74-75 1.0882217496286515 2203 76-77 1.0352305444656835 2203 78-79 0.8435275339945107 2203 80-81 0.8540903905033446 2203 82-83 0.8918256418768387 2203 84-85 1.036780543477061 2203 86-87 1.0211994427233577 2203 88-89 0.9916559779779242 2203 90-91 1.0008257900825868 2203 92-93 1.081406687906604 2203 94-95 1.0338111707304662 2203 96-97 1.000330954615773 2203 98-99 0.9555556007787906 2203 100 0.8708758978851421 2204 1 0.3372623678263409 2204 2 3.8306958834268166 2204 3 2.186414977055268 2204 4 1.0780875002325985 2204 5 0.5734310769205919 2204 6 0.5254000726997532 2204 7 0.5938566980771043 2204 8 0.43150055449472546 2204 9 0.5278273918550269 2204 10-11 0.5216576603070422 2204 12-13 0.5480088017814069 2204 14-15 0.7064582044115326 2204 16-17 0.697268489806639 2204 18-19 0.7766911337633715 2204 20-21 0.7210370057117643 2204 22-23 0.7401831468598985 2204 24-25 0.716478400296019 2204 26-27 0.7404205495585146 2204 28-29 0.7324163662309857 2204 30-31 0.7513863471508841 2204 32-33 0.8989226710438345 2204 34-35 0.8624556002989792 2204 36-37 0.6199657169539137 2204 38-39 0.6033615945381499 2204 40-41 0.5919643045525405 2204 42-43 0.7943307577326024 2204 44-45 0.8610346673551064 2204 46-47 0.7999201539795919 2204 48-49 0.6650536108822394 2204 50-51 0.5750203728467795 2204 52-53 0.6294490180433243 2204 54-55 0.745189411275085 2204 56-57 0.6936956733547106 2204 58-59 0.7726547350719954 2204 60-61 0.7593540056145613 2204 62-63 0.9254563865737993 2204 64-65 0.7568196589603815 2204 66-67 0.571900359987275 2204 68-69 0.6202379550210466 2204 70-71 0.6778733288251289 2204 72-73 0.6308247973216083 2204 74-75 0.6081016297084219 2204 76-77 0.5516911003978464 2204 78-79 0.561999435048044 2204 80-81 0.6299263344986841 2204 82-83 0.6140856636052625 2204 84-85 0.646940648595514 2204 86-87 0.6920612305565612 2204 88-89 0.6282862926364565 2204 90-91 0.7049795663780571 2204 92-93 0.8543804546795108 2204 94-95 0.7013753809515606 2204 96-97 0.7649343506639248 2204 98-99 0.8211715544215075 2204 100 0.5272929470594683 2205 1 0.47131972880798045 2205 2 3.7306689687712584 2205 3 2.161286430027321 2205 4 1.0818932384169742 2205 5 0.6391887244745433 2205 6 0.5204605036513783 2205 7 0.43963185214447975 2205 8 0.3823563871243465 2205 9 0.45835620321778237 2205 10-11 0.5480225822375075 2205 12-13 0.5387999433392494 2205 14-15 0.7590275445599914 2205 16-17 0.6809269111558862 2205 18-19 0.7457808148341698 2205 20-21 0.7452430139091462 2205 22-23 0.7120866270943722 2205 24-25 0.8450193294367025 2205 26-27 0.7365723090152656 2205 28-29 0.8413608898640845 2205 30-31 0.7359665712680794 2205 32-33 0.8302872499691176 2205 34-35 0.8695820534600927 2205 36-37 0.7620336895190505 2205 38-39 0.7818979579875318 2205 40-41 0.7449958037005402 2205 42-43 0.7743638393311087 2205 44-45 0.8235808989226783 2205 46-47 0.734384229517957 2205 48-49 0.597171244961622 2205 50-51 0.5185703633389522 2205 52-53 0.6311815201839082 2205 54-55 0.7607985234948131 2205 56-57 0.5703481286850618 2205 58-59 0.6868433137270813 2205 60-61 0.6157308799402017 2205 62-63 0.6948196389880508 2205 64-65 0.4983542763430435 2205 66-67 0.5562835296251905 2205 68-69 0.5761875092425939 2205 70-71 0.6535357126308128 2205 72-73 0.5803844867637551 2205 74-75 0.5221561608447374 2205 76-77 0.5229358466457228 2205 78-79 0.5818359021224282 2205 80-81 0.5913636141189045 2205 82-83 0.580965369227318 2205 84-85 0.6434910385383539 2205 86-87 0.5681958553166737 2205 88-89 0.6994054834251919 2205 90-91 0.7383834551693411 2205 92-93 0.7506801248687758 2205 94-95 0.6401546972217318 2205 96-97 0.598450444841415 2205 98-99 0.6477574928241658 2205 100 0.5369913502368036 2206 1 0.38494236497649226 2206 2 3.6080518448921133 2206 3 2.135787508817831 2206 4 1.009806692210283 2206 5 0.48252058932944664 2206 6 0.5027829709434783 2206 7 0.39211221456641 2206 8 0.41382353634460145 2206 9 0.4644763688272491 2206 10-11 0.358146244844761 2206 12-13 0.47360361202400014 2206 14-15 0.5470102243449162 2206 16-17 0.5703806044602473 2206 18-19 0.49787926939136895 2206 20-21 0.5059815455778178 2206 22-23 0.5670573021310545 2206 24-25 0.3639138940557771 2206 26-27 0.4632547536450815 2206 28-29 0.43272767553315106 2206 30-31 0.5109408840818332 2206 32-33 0.48939219656568866 2206 34-35 0.6197091994738031 2206 36-37 0.37376552477506664 2206 38-39 0.5737820404224436 2206 40-41 0.6022857503898322 2206 42-43 0.5710367037541957 2206 44-45 0.7017238825223941 2206 46-47 0.5938780893848374 2206 48-49 0.49068851395752944 2206 50-51 0.38724331803528855 2206 52-53 0.36005124539665445 2206 54-55 0.27969545820619857 2206 56-57 0.41891584205440324 2206 58-59 0.3513194348163893 2206 60-61 0.42046405075371496 2206 62-63 0.44420159022757133 2206 64-65 0.30395066413779404 2206 66-67 0.3356093948326304 2206 68-69 0.06058223423092812 2206 70-71 0.35715141218935287 2206 72-73 0.3920466119420496 2206 74-75 0.32674360244293155 2206 76-77 0.33163334291377566 2206 78-79 0.3781754854241228 2206 80-81 0.41372175612426076 2206 82-83 0.3835365753995923 2206 84-85 0.340113015191978 2206 86-87 0.4184889958836209 2206 88-89 0.3960572645139031 2206 90-91 0.3670738042457664 2206 92-93 0.46817762284832654 2206 94-95 0.47473337073686395 2206 96-97 0.4280409969235883 2206 98-99 0.42286977437401774 2206 100 0.21853664538544848 2207 1 0.2898269314960302 2207 2 3.693988761487443 2207 3 2.020641107877701 2207 4 0.8955879269097977 2207 5 0.376953596400071 2207 6 0.3723715156593528 2207 7 0.14548748955188273 2207 8 0.26998469524608737 2207 9 0.2452240031807591 2207 10-11 0.13932451938894985 2207 12-13 0.20503030694091962 2207 14-15 0.26673512556254764 2207 16-17 0.23700338271936516 2207 18-19 0.3255031097527379 2207 20-21 0.41013430182137967 2207 22-23 0.28398232085644537 2207 24-25 0.26368925202923066 2207 26-27 0.3841213834226309 2207 28-29 0.3414849933223252 2207 30-31 0.22659704141914006 2207 32-33 0.40420757800724516 2207 34-35 0.39810774479261113 2207 36-37 0.31715547903216645 2207 38-39 0.19638640444931355 2207 40-41 0.17652340289716761 2207 42-43 0.23092964976795116 2207 44-45 0.3329959910115221 2207 46-47 0.22896257296658007 2207 48-49 0.22889722219250075 2207 50-51 0.08262541435803428 2207 52-53 0.14569647899434557 2207 54-55 0.220583961386275 2207 56-57 0.2597749432069989 2207 58-59 0.2646432654523494 2207 60-61 0.2296400422087359 2207 62-63 0.425836528599973 2207 64-65 0.33701059317990456 2207 66-67 0.3283804347386905 2207 68-69 0.03970855080998348 2207 70-71 0.10612125412167472 2207 72-73 0.2823269798420256 2207 74-75 0.1974465977284936 2207 76-77 0.1915489258127252 2207 78-79 0.25218982551878355 2207 80-81 0.25387122813794605 2207 82-83 0.27357481141509155 2207 84-85 -0.07530046252682965 2207 86-87 0.21294992484553177 2207 88-89 0.2932127396087729 2207 90-91 0.3926733869309835 2207 92-93 0.5245107284190844 2207 94-95 0.37746534257345843 2207 96-97 0.3708609314982212 2207 98-99 0.3898982981037413 2207 100 0.3469791432231517 2208 1 0.33609263086469454 2208 2 3.6359018060451476 2208 3 2.1132790156009484 2208 4 0.8505632186043499 2208 5 0.355980801915031 2208 6 0.32722610544561803 2208 7 0.29328087287768057 2208 8 0.15032760121590627 2208 9 0.24116648253533413 2208 10-11 0.15754837653284426 2208 12-13 0.19789310834656249 2208 14-15 0.24486405977960146 2208 16-17 0.24328333707856586 2208 18-19 0.2870080767583332 2208 20-21 0.26997820036473996 2208 22-23 -0.15050304605438924 2208 24-25 0.1618347170698442 2208 26-27 0.0681052084565863 2208 28-29 0.15656019408699962 2208 30-31 0.17607832734756101 2208 32-33 0.2916161800354189 2208 34-35 0.3235435926800676 2208 36-37 0.3541835514326266 2208 38-39 0.21828658020569947 2208 40-41 0.23365318434478155 2208 42-43 0.15579184290547943 2208 44-45 -0.19157398516464497 2208 46-47 -0.05309722144294682 2208 48-49 0.110977064622098 2208 50-51 0.18946905118060187 2208 52-53 0.19828363564096918 2208 54-55 0.33923358551175653 2208 56-57 0.28946730230448736 2208 58-59 0.2756122013659308 2208 60-61 0.2717635806325589 2208 62-63 0.28751501752959996 2208 64-65 0.2919677609677578 2208 66-67 0.29570108616486124 2208 68-69 0.18788704646366483 2208 70-71 0.1942776741949359 2208 72-73 0.3078804469184817 2208 74-75 0.27258897694640893 2208 76-77 0.28015753322015513 2208 78-79 0.15123321013766855 2208 80-81 0.10357738705936725 2208 82-83 0.2156934671425823 2208 84-85 0.16010605814859602 2208 86-87 0.3257273204442761 2208 88-89 0.2693625266632118 2208 90-91 0.295647714499907 2208 92-93 0.20922517861402312 2208 94-95 0.2778251724652847 2208 96-97 0.1661969013496396 2208 98-99 0.27642865616468626 2208 100 -0.16142561161273505 2209 1 0.41329091836735543 2209 2 3.6690964940830426 2209 3 2.015442302079414 2209 4 0.8302367597867288 2209 5 0.34164682009375724 2209 6 0.16630797696348054 2209 7 0.05234798781290806 2209 8 -0.04941823499213882 2209 9 0.03653360857876464 2209 10-11 -0.016125248215814736 2209 12-13 0.018363729193090705 2209 14-15 0.11480322964030165 2209 16-17 0.17012401184572923 2209 18-19 0.19061816055900493 2209 20-21 0.14596500096845944 2209 22-23 0.1510082195977347 2209 24-25 0.06184574374277929 2209 26-27 0.08467130683856539 2209 28-29 0.1606216784891572 2209 30-31 0.18409614530962415 2209 32-33 0.21988565480094735 2209 34-35 0.26968746732249116 2209 36-37 0.1276785889202472 2209 38-39 0.12261182833131556 2209 40-41 0.12446947406296971 2209 42-43 0.22162792866079428 2209 44-45 0.12157561784456306 2209 46-47 -0.07553554290205255 2209 48-49 -0.08189491488990086 2209 50-51 -0.028350031893452865 2209 52-53 0.09349131314034054 2209 54-55 0.06528109396266046 2209 56-57 -0.052574425805474334 2209 58-59 0.03830126243759935 2209 60-61 -0.373619861218053 2209 62-63 0.026103379192242926 2209 64-65 -9.833415466715678E-4 2209 66-67 0.04971050469639948 2209 68-69 0.060349795773980475 2209 70-71 0.00659424365635175 2209 72-73 0.12574263796526708 2209 74-75 0.1281905369862386 2209 76-77 0.017949324769162445 2209 78-79 -3.2125520627346305E-4 2209 80-81 -0.02695803842040334 2209 82-83 -0.24517260565995258 2209 84-85 -0.10993310590322736 2209 86-87 0.13919991732766235 2209 88-89 0.06512143589901953 2209 90-91 0.1905273841481261 2209 92-93 0.23727681034760906 2209 94-95 0.11751241036524362 2209 96-97 0.12438202512455376 2209 98-99 0.1750818734156212 2209 100 0.11166145935847993 2210 1 0.43785962830231995 2210 2 3.8348304692351327 2210 3 2.14913171720573 2210 4 0.9774370511263797 2210 5 0.4102162210266016 2210 6 0.2491782327727634 2210 7 0.23342211601169538 2210 8 0.05821402340372117 2210 9 0.23335408452690132 2210 10-11 0.15344329830747938 2210 12-13 0.18377601681628875 2210 14-15 0.16657064348733286 2210 16-17 0.22595570717168556 2210 18-19 0.21445618691964086 2210 20-21 0.186962652509429 2210 22-23 0.20816868518090104 2210 24-25 0.18146167964805926 2210 26-27 0.20447913472406043 2210 28-29 0.14555559014413433 2210 30-31 0.14381003483400434 2210 32-33 0.22107669237162497 2210 34-35 0.29674003451970066 2210 36-37 0.16684696362786156 2210 38-39 0.15693247936337684 2210 40-41 0.09870085563853337 2210 42-43 0.2225276265826679 2210 44-45 0.25140198128143965 2210 46-47 0.33596817121158296 2210 48-49 0.0742762098025338 2210 50-51 0.03263986926583584 2210 52-53 0.16678377799516397 2210 54-55 0.22063796168792038 2210 56-57 0.15801091476777174 2210 58-59 0.2137456254355925 2210 60-61 0.15350460711042757 2210 62-63 0.18227777211791363 2210 64-65 -0.30671021996372616 2210 66-67 -0.026580330710707756 2210 68-69 0.17083148666448977 2210 70-71 0.15410268270147043 2210 72-73 0.20518405021553576 2210 74-75 -0.4536922454941603 2210 76-77 0.07199376340341246 2210 78-79 0.08123050193598402 2210 80-81 0.09797904808973357 2210 82-83 0.17648037333464472 2210 84-85 0.24468514110984074 2210 86-87 0.2930426592819728 2210 88-89 0.3875401152526585 2210 90-91 0.22390911845943862 2210 92-93 0.3114727082480009 2210 94-95 0.16099251849707485 2210 96-97 0.15036961517605008 2210 98-99 0.11932768190649057 2210 100 0.00781755017801089 2211 1 0.5045327292733397 2211 2 3.9492088614823153 2211 3 2.1772666121966857 2211 4 1.029779597504323 2211 5 0.5094861872524987 2211 6 0.3192384554636405 2211 7 0.221290055311826 2211 8 0.29359626304518827 2211 9 0.31105646238202667 2211 10-11 0.10850839468416495 2211 12-13 0.16284254712365964 2211 14-15 0.23541202509633763 2211 16-17 0.2228215015704862 2211 18-19 0.3311292279437268 2211 20-21 0.3557375529675397 2211 22-23 0.22733449960197305 2211 24-25 0.2771559910436068 2211 26-27 0.21513248437894816 2211 28-29 0.26452813564686295 2211 30-31 0.25125256670479246 2211 32-33 0.33585055454882706 2211 34-35 0.457696775612483 2211 36-37 0.304275731682381 2211 38-39 0.15204884235885174 2211 40-41 0.20839223682790475 2211 42-43 0.4795775060293792 2211 44-45 0.41065017478054955 2211 46-47 0.4927243706238613 2211 48-49 0.30424626031382473 2211 50-51 0.2290760839286179 2211 52-53 0.3003650318692266 2211 54-55 0.28031051953970376 2211 56-57 0.24589749756467683 2211 58-59 0.16157152994492208 2211 60-61 0.2084142610847337 2211 62-63 0.37264939515720386 2211 64-65 0.2856269431794587 2211 66-67 0.41883966351041124 2211 68-69 0.41352503433608945 2211 70-71 0.20011243486885633 2211 72-73 0.23927467319607842 2211 74-75 0.28474644857384845 2211 76-77 0.3030945330021595 2211 78-79 0.28855517813261855 2211 80-81 0.293530985514721 2211 82-83 -0.06958776714740011 2211 84-85 0.27435809461140437 2211 86-87 0.3332691507916863 2211 88-89 0.47609408727661773 2211 90-91 0.38471305481497353 2211 92-93 0.29508231702231313 2211 94-95 0.4755779674576601 2211 96-97 0.3401535784117975 2211 98-99 0.4049609793116282 2211 100 0.4440971954360293 2212 1 0.8294267534912656 2212 2 4.2724886554332 2212 3 2.5386037851026586 2212 4 1.35637281147703 2212 5 0.9108348073315611 2212 6 0.8198913020536338 2212 7 0.7833507253291714 2212 8 0.7189258164265766 2212 9 0.6083162953248404 2212 10-11 0.7723997180846993 2212 12-13 0.6625228311566431 2212 14-15 0.9032763224366533 2212 16-17 0.9457529616216434 2212 18-19 0.8789211755729127 2212 20-21 0.8755621786986438 2212 22-23 0.8642757791812912 2212 24-25 0.8261172495172033 2212 26-27 0.677169008015575 2212 28-29 0.7554240558028269 2212 30-31 0.7370844542363173 2212 32-33 0.8123167764451225 2212 34-35 0.6449318361572978 2212 36-37 0.870094741144186 2212 38-39 0.8880238195539363 2212 40-41 0.784178335667093 2212 42-43 0.8764724603301133 2212 44-45 0.9148802629595565 2212 46-47 0.8862254169670294 2212 48-49 0.9241799919133911 2212 50-51 0.9958153387891926 2212 52-53 0.9043487375337875 2212 54-55 0.6649221205341789 2212 56-57 0.803759380266925 2212 58-59 0.8296415186766097 2212 60-61 0.7460378994971606 2212 62-63 0.7826035170338272 2212 64-65 0.9441971197163284 2212 66-67 0.9552582186625287 2212 68-69 0.9235323676813465 2212 70-71 0.9633348140922351 2212 72-73 0.9594136490318093 2212 74-75 0.947996104264341 2212 76-77 0.9711578144058706 2212 78-79 1.0795324805347306 2212 80-81 0.9593897917892065 2212 82-83 0.9330325623165443 2212 84-85 1.082960019167377 2212 86-87 0.8226089708564359 2212 88-89 0.9756603467339566 2212 90-91 0.856466260917756 2212 92-93 0.7843103482874021 2212 94-95 0.9859609111697623 2212 96-97 0.8319348658610473 2212 98-99 0.9445744583579305 2212 100 0.782848559259385 2213 1 -0.7165752288327596 2213 2 -25.643692102584293 2213 3 -14.637719965979755 2213 4 -6.61274768114118 2213 5 -2.564480066054749 2213 6 -1.7728251080155957 2213 7 -1.5424082619445514 2213 8 -1.424534194233381 2213 9 -1.4467642117713595 2213 10-11 -1.3572118960738067 2213 12-13 -1.581279924548646 2213 14-15 -1.8515185574250381 2213 16-17 -1.9625620705949416 2213 18-19 -1.7079368408507563 2213 20-21 -1.8667744513810618 2213 22-23 -1.8598505397590586 2213 24-25 -1.9435313155887073 2213 26-27 -1.8750536995213807 2213 28-29 -2.014157884583895 2213 30-31 -2.0861498570740764 2213 32-33 -1.970395769772196 2213 34-35 -2.1107133202065285 2213 36-37 -1.9660365122252301 2213 38-39 -2.1645175030440953 2213 40-41 -2.043626545920354 2213 42-43 -1.8665038815948165 2213 44-45 -1.7893372917567163 2213 46-47 -1.9577211641349166 2213 48-49 -1.8775629794650897 2213 50-51 -1.7990265334532296 2213 52-53 -1.8051287337660327 2213 54-55 -1.880094045028052 2213 56-57 -1.822063597281499 2213 58-59 -1.9211428967134339 2213 60-61 -1.9525194176151288 2213 62-63 -1.9442857232840218 2213 64-65 -1.8440855584675617 2213 66-67 -1.8319861447716832 2213 68-69 -1.897073798904124 2213 70-71 -1.8049073776434028 2213 72-73 -1.9604637309406066 2213 74-75 -2.051011015608225 2213 76-77 -2.047330640618437 2213 78-79 -1.8470020261063596 2213 80-81 -2.11944500486986 2213 82-83 -2.05932983950267 2213 84-85 -2.0891992097116336 2213 86-87 -2.0046207236854556 2213 88-89 -2.0330787546837605 2213 90-91 -1.9123261097310262 2213 92-93 -1.7708610280532966 2213 94-95 -1.8363156459396492 2213 96-97 -1.7125712101606254 2213 98-99 -1.8280958909312197 2213 100 -1.9007810027203114 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3.0 3 10.0 4 22.0 5 29.0 6 32.0 7 26.0 8 42.0 9 82.0 10 415.0 11 1924.0 12 5808.0 13 10503.0 14 13837.0 15 15565.0 16 16297.0 17 16525.0 18 16668.0 19 17427.0 20 18817.0 21 20169.0 22 22361.0 23 23392.0 24 24417.0 25 25066.0 26 27208.0 27 30994.0 28 35742.0 29 41924.0 30 50222.0 31 60631.0 32 76364.0 33 100029.0 34 134552.0 35 187574.0 36 279978.0 37 469597.0 38 495405.0 39 4664.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 38.03882633181338 21.13840661316088 10.069522213758926 30.753244841266813 2 12.364590946799934 23.24774915571307 36.54111448225294 27.846545415234065 3 14.759965263436023 28.135190999861425 29.04923137109175 28.0556123656108 4 10.012837428076315 19.032233485234727 38.294079142900046 32.66084994378891 5 11.04521401580489 38.955254587965634 35.633478660007434 14.366052736222045 6 28.87653378164807 40.61711041177102 17.913374856836644 12.592980949744256 7 24.899310551398422 33.66504244200662 24.136540062722528 17.29910694387243 8 23.710969817134053 37.52277800758851 21.3583270333495 17.407925141927937 9 24.37431967042852 16.73871510984676 22.42606973885151 36.46089548087321 10-11 22.48506387885817 28.156711258467666 29.536856547711114 19.821368314963046 12-13 23.19884212393767 25.839388825444033 29.93978384768242 21.021985202935877 14-15 20.97180983439654 27.052814714998096 26.97823944412226 24.9971360064831 16-17 20.13918866486879 30.815638533794417 27.959926429867355 21.08524637146944 18-19 20.088670598420688 29.70224372937974 30.070163201590233 20.13892247060934 20-21 21.34795932283157 29.06742715750949 29.10958751482966 20.475026004829278 22-23 20.404025818504806 29.18976904087989 29.421806680313058 20.984398460302245 24-25 20.698393061347815 28.787032720796972 29.184288562650462 21.330285655204747 26-27 20.643803006698587 28.885219441939057 29.214801234906794 21.256176316455562 28-29 20.252114434844923 28.789872104434227 29.811191597344223 21.146821863376626 30-31 21.155115916047077 29.140025158690275 28.91381978667481 20.791039138587838 32-33 19.94989045499164 29.235083097808968 29.762400731102094 21.0526257160973 34-35 20.197798863272556 28.57890800465813 29.847260743513587 21.37603238855573 36-37 20.921649807472555 29.087183975050635 29.063500971716078 20.927665245760732 38-39 20.542456729164652 29.018340337379506 29.43042867792631 21.008774255529527 40-41 20.794569930085512 28.88032100954919 29.136072579171813 21.189036481193494 42-43 20.42008080070815 29.064638115716612 29.414813136386897 21.10046794718834 44-45 20.90747923647349 28.417128257846713 29.312371178351437 21.36302132732836 46-47 20.835467773939065 29.190100949106544 29.122385611994904 20.852045664959483 48-49 20.646746046973476 29.014279853575847 29.252362835433722 21.08661126401696 50-51 21.02563954009178 28.833285275815246 28.78363822003272 21.357436964060255 52-53 20.377718490997935 29.21841879602872 28.913633146559174 21.49022956641417 54-55 20.815037437831094 28.995829148628893 28.65112166420468 21.538011749335332 56-57 21.011106218983517 28.873317266556388 28.645729350864187 21.469847163595915 58-59 20.457360019500815 28.52989894425557 29.345448258006098 21.66729277823752 60-61 21.142020523901586 28.896558992347153 28.511811035902902 21.44960944784836 62-63 20.300879375290368 29.232939194646875 29.03975070440267 21.42643072566009 64-65 20.404232439223193 28.50840074922891 29.173468419145713 21.91389839240218 66-67 20.71251805460792 28.892096616273456 28.800336202313222 21.595049126805403 68-69 20.84914784375947 28.797109264970672 28.85313229814772 21.500610593122136 70-71 21.042629399415212 28.678603828505455 28.473253030734874 21.80551374134446 72-73 20.581309065141287 29.049988524009336 28.966470052121053 21.402232358728327 74-75 21.055839251126883 28.326012412882086 28.704411003129515 21.913737332861512 76-77 20.845324001501826 28.970924927554638 28.73319496018708 21.450556110756448 78-79 20.784423647414435 28.824647941615417 29.010252596125607 21.38067581484454 80-81 21.0279721012041 28.66319866472939 28.544640145594585 21.764189088471927 82-83 20.306052357710026 29.04574253556511 28.882006328875615 21.766198777849247 84-85 20.559412077682605 28.455830517809765 28.723888265527563 22.260869138980066 86-87 20.82262583088511 28.810864548293047 28.517166686793132 21.849342934028716 88-89 20.49166028532133 28.42367439386874 29.00983726065499 22.07482806015494 90-91 20.881488675432045 28.49490646762644 28.61361407861572 22.00999077832579 92-93 20.16998661682341 28.945692196741383 28.937872161811896 21.94644902462331 94-95 20.233507939252114 28.258421809639746 29.110984666879354 22.39708558422879 96-97 20.58807475716886 28.62957889218785 28.761579579259784 22.0207667713835 98-99 20.451577343781675 28.409944076378007 28.842014319762278 22.296464260078036 100 20.624848059059026 28.283920466584146 28.648088053260178 22.44314342109665 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 29.0 1 24.0 2 22.0 3 26.5 4 25.5 5 32.0 6 63.0 7 107.5 8 166.0 9 205.0 10 189.0 11 165.0 12 211.0 13 287.5 14 353.0 15 425.5 16 532.5 17 655.5 18 847.5 19 1150.0 20 1587.0 21 2271.5 22 3276.5 23 4880.0 24 7068.5 25 10030.5 26 14386.5 27 20064.5 28 26796.5 29 34485.5 30 42546.5 31 50504.5 32 58348.5 33 65750.5 34 72775.0 35 79248.0 36 84635.0 37 89441.5 38 93467.5 39 95576.5 40 96320.0 41 95628.0 42 93313.0 43 89792.0 44 85596.5 45 81187.0 46 76494.5 47 72387.0 48 68942.0 49 67441.0 50 72855.5 51 69031.0 52 54941.5 53 47607.0 54 43375.0 55 39883.5 56 36571.0 57 32853.5 58 28894.5 59 25171.0 60 21472.5 61 17620.5 62 14330.0 63 11591.5 64 9116.0 65 7219.0 66 5617.0 67 4318.0 68 3304.0 69 2456.5 70 1855.0 71 1348.0 72 1005.5 73 754.5 74 518.0 75 336.0 76 205.5 77 141.5 78 92.0 79 46.5 80 20.5 81 10.5 82 7.0 83 3.5 84 1.0 85 0.5 86 0.5 87 1.0 88 0.5 89 0.0 90 0.0 91 0.5 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content warn #Base N-Count 1 0.7307332596362107 2 5.2036228329191765 3 0.0 4 0.004544804419688627 5 0.16138511380502166 6 0.05587436021852489 7 0.03515539889347379 8 0.041393365744026814 9 0.04112602430757454 10-11 0.041616150274403706 12-13 0.040234886186066965 14-15 0.042329060771609764 16-17 0.033306287291345577 18-19 0.02778123093799862 20-21 0.022590351380217002 22-23 0.022456680661990865 24-25 0.019025798894186704 26-27 0.011807580109975357 28-29 0.020451619888598824 30-31 0.0251078165734759 32-33 0.0016931624308643906 34-35 0.003185818784389577 36-37 0.004255184530198666 38-39 0.006327080662703775 40-41 0.006728092817382184 42-43 0.007017712706872145 44-45 0.008087078452681234 46-47 0.01641921988877705 48-49 0.025196930385626655 50-51 0.021476428728332532 52-53 0.027313383424207142 54-55 0.023815666297289914 56-57 0.015728587844608684 58-59 0.013901754695518154 60-61 0.011339732596183878 62-63 0.01775592707103841 64-65 0.018446559115206783 66-67 0.019337697236714354 68-69 0.026778700551302596 70-71 0.026711865192189533 72-73 0.022545794474141623 74-75 0.02330326187742306 76-77 0.017310358010284625 78-79 0.013545299446915125 80-81 0.012542769060219104 82-83 0.007240497237249039 84-85 0.00957973480620642 86-87 0.007931129281417409 88-89 0.006661257458269115 90-91 0.006750371270419872 92-93 0.003809615469444879 94-95 0.0012921502761859822 96-97 6.460751380929911E-4 98-99 4.45569060753787E-4 100 0.11121403756414523 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 2244321.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 88.24459660048869 #Duplication Level Percentage of deduplicated Percentage of total 1 92.64790742379411 81.75677216492132 2 5.921382869746058 10.450600853155699 3 0.8412506683346653 2.2270747760125214 4 0.24252469597311793 0.8560597584721582 5 0.09827277578922705 0.4336020728165305 6 0.0644149320869878 0.3410561818238468 7 0.03643774671079898 0.22508039816676678 8 0.02807904582239735 0.1982259257219273 9 0.01656930283315808 0.1315936300017032 >10 0.09561451509637552 1.4538094951309861 >50 0.004147662459034683 0.2506168307906147 >100 0.0024865769757832076 0.48760475368962675 >500 3.5458281381539164E-4 0.19033270563815693 >1k 5.065468768720206E-4 0.7420204118262272 >5k 5.0654687687202064E-5 0.25555004183196034 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 5717 0.25473183203294003 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 4201 0.18718356242266593 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 4.45569060753787E-5 0.0 0.0 4.45569060753787E-5 0.0 6 4.45569060753787E-5 0.0 0.0 4.45569060753787E-5 0.0 7 4.45569060753787E-5 0.0 0.0 4.45569060753787E-5 0.0 8 4.45569060753787E-5 0.0 0.0 8.91138121507574E-5 0.0 9 4.45569060753787E-5 0.0 0.0 1.336707182261361E-4 0.0 10-11 4.45569060753787E-5 0.0 0.0 1.782276243015148E-4 0.0 12-13 4.45569060753787E-5 0.0 0.0 1.782276243015148E-4 0.0 14-15 4.45569060753787E-5 0.0 0.0 2.227845303768935E-4 0.0 16-17 4.45569060753787E-5 0.0 0.0 2.673414364522722E-4 0.0 18-19 4.45569060753787E-5 4.45569060753787E-5 0.0 2.673414364522722E-4 0.0 20-21 4.45569060753787E-5 6.683535911306805E-5 0.0 3.564552486030296E-4 0.0 22-23 6.683535911306805E-5 8.91138121507574E-5 0.0 4.4556906075378697E-4 0.0 24-25 8.91138121507574E-5 8.91138121507574E-5 0.0 6.906320441683699E-4 0.0 26-27 8.91138121507574E-5 8.91138121507574E-5 0.0 8.020243093568166E-4 0.0 28-29 8.91138121507574E-5 8.91138121507574E-5 0.0 0.0016040486187136331 0.0 30-31 1.336707182261361E-4 8.91138121507574E-5 0.0 0.00478986740310321 0.0 32-33 1.336707182261361E-4 8.91138121507574E-5 0.0 0.013166565745274406 0.0 34-35 1.782276243015148E-4 8.91138121507574E-5 0.0 0.025620220993342754 0.0 36-37 1.782276243015148E-4 8.91138121507574E-5 0.0 0.04544804419688628 0.0 38-39 1.782276243015148E-4 8.91138121507574E-5 0.0 0.07320699668184721 0.0 40-41 2.0050607733920414E-4 8.91138121507574E-5 0.0 0.10820644640405717 0.0 42-43 2.227845303768935E-4 8.91138121507574E-5 0.0 0.14137906297717662 0.0 44-45 2.227845303768935E-4 8.91138121507574E-5 0.0 0.18181445524058276 0.0 46-47 2.227845303768935E-4 8.91138121507574E-5 0.0 0.23098300109476316 0.0 48-49 2.227845303768935E-4 8.91138121507574E-5 0.0 0.2826244552361271 0.0 50-51 2.227845303768935E-4 8.91138121507574E-5 0.0 0.3361150209796192 0.0 52-53 2.227845303768935E-4 8.91138121507574E-5 0.0 0.3926131778831994 0.0 54-55 2.227845303768935E-4 8.91138121507574E-5 0.0 0.45726524859857387 0.0 56-57 2.673414364522722E-4 8.91138121507574E-5 0.0 0.5349947712470721 0.0 58-59 2.673414364522722E-4 8.91138121507574E-5 0.0 0.6231506099172088 0.0 60-61 2.673414364522722E-4 8.91138121507574E-5 0.0 0.7148041657142628 0.0 62-63 2.673414364522722E-4 8.91138121507574E-5 0.0 0.8087524021741987 0.0 64-65 2.896198894899616E-4 8.91138121507574E-5 0.0 0.9152211292413162 0.0 66-67 3.118983425276509E-4 8.91138121507574E-5 0.0 1.0409161612799593 0.0 68-69 3.118983425276509E-4 8.91138121507574E-5 0.0 1.1822061104449855 0.0 70-71 3.118983425276509E-4 8.91138121507574E-5 0.0 1.347534510437678 0.0 72-73 3.118983425276509E-4 8.91138121507574E-5 0.0 1.5116598739663354 0.0 74-75 3.118983425276509E-4 8.91138121507574E-5 0.0 1.6779685258926866 0.0 76-77 3.564552486030296E-4 8.91138121507574E-5 0.0 1.8611196883155305 0.0 78-79 3.564552486030296E-4 1.1139226518844676E-4 0.0 2.0711609435548657 0.0 80-81 3.564552486030296E-4 1.336707182261361E-4 0.0 2.3092730496216896 0.0 82-83 3.564552486030296E-4 1.336707182261361E-4 0.0 2.5634479203286875 0.0 84-85 3.564552486030296E-4 1.336707182261361E-4 0.0 2.8262445523612714 0.0 86-87 3.564552486030296E-4 1.336707182261361E-4 0.0 3.1083120462714557 0.0 88 3.564552486030296E-4 1.336707182261361E-4 0.0 3.343282890459965 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GAGTACT 2955 0.0 16.604805 12-13 CCTATAC 600 0.0 15.672032 3 GGACCGT 180 0.0028269782 15.653512 6 GTATAGG 530 5.0931703E-11 14.98389 1 TATGCCG 565 1.8189894E-12 14.964549 94 ATACGAT 220 6.2202313E-4 14.94199 6 CATGGGT 1775 0.0 14.834239 4 ACTCCGC 225 7.3830923E-4 14.607991 8 CTAGGAC 775 0.0 14.559822 3 GTATCAA 10385 0.0 14.4816 1 CTATACT 950 0.0 14.353243 4 GTCTAGC 520 6.148184E-10 14.317537 1 AGTACTT 3550 0.0 13.821745 12-13 GTACTTT 3655 0.0 13.745383 14-15 CAACGCA 10910 0.0 13.66187 5 AACGCAG 10895 0.0 13.577393 6 ATCAACG 11090 0.0 13.566411 3 TCAACGC 11075 0.0 13.54324 4 GTACATG 12100 0.0 13.536581 1 TTGGCGT 280 2.4898967E-4 13.434069 4 >>END_MODULE