FastQCFastQC Report
Wed 25 May 2016
SRR1295086_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295086_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3253320
Sequences flagged as poor quality0
Sequence length100
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT36940.11354554731781688No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTACGC1853.792331E-521.5358243
CGACCGA1200.00328458921.1049946
GTGCGCG1859.220195E-519.1877449
GTGTATA19550.018.0595341
ACCGATC2403.5251178E-516.8974118
GTATATA26550.016.8600581
GTATAGG8150.016.245271
GTGTAGA13350.016.0569461
CTATACT13500.016.0221924
GTCTAGG9900.015.9209891
GTATTAG13600.015.7617791
GTACATG140600.015.6945171
CATGGGG41350.015.2798654
TACATGG143050.015.1129382
GTATAGA12150.015.0482851
GTCTATA10150.014.9076921
ACATGGG140900.014.501693
GTCTAGA9650.014.3734371
GTACATA16100.013.7058951
TAATCCG3404.5846187E-513.3997745
Produced by FastQC (version 0.11.5)