##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1295086_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3253320 Sequences flagged as poor quality 0 Sequence length 100 %GC 42 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 25.527799294259403 27.0 22.0 33.0 6.0 33.0 2 21.54294966372813 27.0 2.0 33.0 2.0 33.0 3 23.93223660752708 27.0 15.0 33.0 6.0 33.0 4 23.699599793441777 33.0 2.0 37.0 2.0 37.0 5 25.70139057946959 33.0 15.0 37.0 2.0 37.0 6 26.62487735605474 33.0 15.0 37.0 2.0 37.0 7 26.494604588543396 33.0 15.0 37.0 6.0 37.0 8 27.126851954311288 33.0 15.0 37.0 2.0 37.0 9 28.16897261874024 33.0 15.0 37.0 6.0 37.0 10-11 27.70284079033111 33.0 15.0 37.0 4.0 37.0 12-13 26.47545722523453 33.0 15.0 37.0 2.0 37.0 14-15 28.151299902868455 33.0 15.0 37.0 4.0 40.0 16-17 28.582517704990593 33.0 18.5 37.0 4.0 40.0 18-19 28.84007844294444 33.0 18.5 38.5 6.0 40.0 20-21 28.298896050803485 33.0 15.0 37.0 4.0 40.0 22-23 29.09005400636888 33.0 22.0 37.0 6.0 40.0 24-25 29.263307482817552 33.0 24.5 37.0 6.0 40.0 26-27 27.753710363567066 33.0 15.0 37.0 4.0 40.0 28-29 28.492119434915715 33.0 15.0 37.0 6.0 40.0 30-31 29.26203893253661 33.0 22.0 37.0 6.0 40.0 32-33 27.937219824671413 33.0 18.5 37.0 4.0 40.0 34-35 27.59834507518473 33.0 15.0 37.0 6.0 40.0 36-37 27.890170502747964 33.0 18.5 37.0 6.0 40.0 38-39 28.009628779216307 33.0 18.5 37.0 6.0 40.0 40-41 27.107367858064993 33.0 15.0 37.0 4.0 40.0 42-43 27.717149250611683 33.0 15.0 37.0 6.0 40.0 44-45 27.625471364636738 33.0 15.0 37.0 6.0 40.0 46-47 27.376051080127375 33.0 15.0 37.0 6.0 40.0 48-49 27.381191367587572 33.0 15.0 37.0 6.0 40.0 50-51 27.282810329140695 33.0 15.0 37.0 6.0 40.0 52-53 27.516745048135444 33.0 18.5 37.0 6.0 40.0 54-55 27.47005612727921 33.0 22.0 37.0 6.0 40.0 56-57 27.47291489924139 33.0 22.0 37.0 6.0 40.0 58-59 27.390928343968625 33.0 22.0 37.0 6.0 40.0 60-61 27.334464946577647 33.0 22.0 37.0 6.0 40.0 62-63 27.399371872425707 33.0 22.0 37.0 6.0 40.0 64-65 26.958106488141347 33.0 18.5 37.0 6.0 40.0 66-67 27.138597647941182 33.0 22.0 37.0 6.0 40.0 68-69 26.677117682859354 33.0 18.5 37.0 6.0 38.5 70-71 26.852925626744373 30.0 18.5 37.0 6.0 37.0 72-73 26.94852489149546 27.0 22.0 37.0 6.0 37.0 74-75 27.078084695019243 27.0 22.0 37.0 6.0 37.0 76-77 26.840100574182678 27.0 22.0 37.0 6.0 37.0 78-79 26.399391390948324 27.0 22.0 33.0 6.0 37.0 80-81 26.219181021233695 27.0 22.0 33.0 6.0 37.0 82-83 26.351088119213603 27.0 22.0 33.0 6.0 37.0 84-85 26.301281306480764 27.0 22.0 33.0 6.0 37.0 86-87 26.146078621223857 27.0 22.0 33.0 6.0 37.0 88-89 26.13390859183849 27.0 22.0 33.0 6.0 37.0 90-91 26.28743391366358 27.0 22.0 33.0 6.0 37.0 92-93 26.160178217943518 27.0 22.0 33.0 6.0 37.0 94-95 26.034929702580747 27.0 22.0 33.0 6.0 37.0 96-97 26.114045651826444 27.0 22.0 33.0 6.0 37.0 98-99 26.216110465616662 27.0 22.0 33.0 10.5 37.0 100 25.82703730343157 27.0 22.0 33.0 6.0 37.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 7.335386494803142 1101 2 11.22671025280998 1101 3 8.403334083275972 1101 4 12.664461266329244 1101 5 10.751868365415067 1101 6 9.948974572644552 1101 7 9.4713741071586 1101 8 9.003575104610324 1101 9 7.435791501414979 1101 10-11 7.7827120177344895 1101 12-13 9.186122628501089 1101 14-15 8.168286190971333 1101 16-17 8.296881154261573 1101 18-19 8.193358408182394 1101 20-21 7.355985231984452 1101 22-23 7.486013700725575 1101 24-25 7.305186235431069 1101 26-27 8.525155770649857 1101 28-29 8.2652494103768 1101 30-31 5.68706891863884 1101 32-33 7.135134318964045 1101 34-35 8.797041929484823 1101 36-37 8.37251337862736 1101 38-39 8.303118671790294 1101 40-41 8.984844098009045 1101 42-43 8.62625846806484 1101 44-45 8.529254817743535 1101 46-47 7.436932093661529 1101 48-49 5.71792235583203 1101 50-51 6.971349642029818 1101 52-53 6.103237355453569 1101 54-55 4.337994834186439 1101 56-57 6.418231155216425 1101 58-59 6.448525439279365 1101 60-61 6.984612102395385 1101 62-63 6.4517176998873325 1101 64-65 6.91361225964393 1101 66-67 6.105577919314051 1101 68-69 7.15529819262083 1101 70-71 6.667917077316126 1101 72-73 5.828348699450633 1101 74-75 5.374109152178583 1101 76-77 5.517315411313131 1101 78-79 5.463027537701095 1101 80-81 5.2863477177794 1101 82-83 4.816220917991711 1101 84-85 4.622812743633656 1101 86-87 4.437782934660532 1101 88-89 4.387814285171977 1101 90-91 4.199638986834142 1101 92-93 4.11325168278378 1101 94-95 3.990237373367279 1101 96-97 4.116711313195886 1101 98-99 3.843334263557626 1101 100 4.088254849477821 1102 1 6.75798753649223 1102 2 10.44132092717388 1102 3 8.13139293662853 1102 4 12.442545219021387 1102 5 10.49909943072429 1102 6 9.550160516719345 1102 7 6.62804604223912 1102 8 7.815130438679862 1102 9 6.457734151184187 1102 10-11 7.532381515343808 1102 12-13 9.130987909787919 1102 14-15 6.496367404517418 1102 16-17 7.458699878849448 1102 18-19 7.3571084486661 1102 20-21 7.051795418080761 1102 22-23 6.674489636059018 1102 24-25 7.029898122750534 1102 26-27 8.568047210546862 1102 28-29 7.715682344089853 1102 30-31 4.370560200454374 1102 32-33 4.632075874080641 1102 34-35 5.107515809352339 1102 36-37 5.299429318537449 1102 38-39 4.580606225997947 1102 40-41 6.2377973970104 1102 42-43 6.948321696138741 1102 44-45 6.472117840257432 1102 46-47 5.551476934644764 1102 48-49 3.8276172319480892 1102 50-51 5.132997446585815 1102 52-53 5.876677812978354 1102 54-55 5.484811844768487 1102 56-57 6.3996726080872115 1102 58-59 5.980884331739912 1102 60-61 6.121536906588911 1102 62-63 4.311079100896059 1102 64-65 6.268833146907763 1102 66-67 4.177263752068431 1102 68-69 6.286395108749158 1102 70-71 5.450202283283254 1102 72-73 5.237443035395017 1102 74-75 4.394269624787782 1102 76-77 3.4552199536264077 1102 78-79 4.514613961469543 1102 80-81 4.556849678420139 1102 82-83 4.103500969648135 1102 84-85 3.208064870573306 1102 86-87 3.4788503782031093 1102 88-89 3.541718352834046 1102 90-91 3.3678846018926833 1102 92-93 3.390404113479196 1102 94-95 3.2458811262222333 1102 96-97 3.4963297442268804 1102 98-99 3.2686301734727756 1102 100 3.442296104660713 1103 1 6.582615911914907 1103 2 10.868348300353414 1103 3 7.722866090636828 1103 4 11.756659263864368 1103 5 10.114395544326275 1103 6 8.859802619770363 1103 7 7.838846492851676 1103 8 7.894925457769563 1103 9 6.9367734742551725 1103 10-11 7.207109161272015 1103 12-13 8.491741154766558 1103 14-15 8.364474423646808 1103 16-17 7.950233034860066 1103 18-19 7.06938843004993 1103 20-21 8.163736797409772 1103 22-23 6.722871803040011 1103 24-25 5.095680802758796 1103 26-27 5.867787686666471 1103 28-29 6.855587779491071 1103 30-31 5.976130623097557 1103 32-33 7.653888067587889 1103 34-35 7.087600206757255 1103 36-37 4.153321613026641 1103 38-39 6.21790557668654 1103 40-41 6.634283559344567 1103 42-43 7.41760949875313 1103 44-45 5.6391655793928095 1103 46-47 6.374785130724366 1103 48-49 5.532674740067861 1103 50-51 6.3471417179166245 1103 52-53 6.149570290562725 1103 54-55 4.991951226195418 1103 56-57 5.905767230861546 1103 58-59 5.383946113733991 1103 60-61 5.053261178334619 1103 62-63 2.783115484522842 1103 64-65 5.020273003299813 1103 66-67 4.2292147641878906 1103 68-69 5.160456565591463 1103 70-71 3.710834588487579 1103 72-73 4.382827233108944 1103 74-75 3.329412860683817 1103 76-77 3.634084344140458 1103 78-79 2.9842527679054704 1103 80-81 4.115147700541286 1103 82-83 3.499177330437629 1103 84-85 3.314391101978547 1103 86-87 2.7674979925992638 1103 88-89 3.0310364067397906 1103 90-91 2.756114998307261 1103 92-93 2.3745838448433965 1103 94-95 2.83325820216524 1103 96-97 3.007780802796592 1103 98-99 2.8383429959074356 1103 100 2.918306217286794 1104 1 6.292008456725316 1104 2 9.678983255828712 1104 3 7.41611632146871 1104 4 12.135707574392658 1104 5 9.4217408053341 1104 6 8.104940139338918 1104 7 4.277537181755601 1104 8 6.589684576309221 1104 9 6.597228616681615 1104 10-11 7.005371278358652 1104 12-13 7.472143686570565 1104 14-15 7.324672091020329 1104 16-17 7.182180439656651 1104 18-19 6.823862351035583 1104 20-21 7.581893720461576 1104 22-23 6.522384448455025 1104 24-25 6.082597056588607 1104 26-27 8.180478565133708 1104 28-29 7.1710523819432375 1104 30-31 6.3238179506539005 1104 32-33 7.653257180014354 1104 34-35 7.882969442535927 1104 36-37 6.618996118302668 1104 38-39 7.155683595323609 1104 40-41 7.205977697575225 1104 42-43 7.328000901648643 1104 44-45 6.798376996002183 1104 46-47 6.675767231956616 1104 48-49 5.531938118793597 1104 50-51 5.763640052661859 1104 52-53 6.028602932639981 1104 54-55 5.055307594343702 1104 56-57 6.1278505180889695 1104 58-59 3.9470558908474374 1104 60-61 4.291066202941568 1104 62-63 3.8574267982782473 1104 64-65 2.826859262771176 1104 66-67 3.326997712378148 1104 68-69 4.382158662760347 1104 70-71 2.7500028590352414 1104 72-73 2.594625418017525 1104 74-75 3.3854160542361242 1104 76-77 4.026138512084483 1104 78-79 4.413672903701741 1104 80-81 3.215809494191184 1104 82-83 3.112244183704739 1104 84-85 3.1532936210845364 1104 86-87 0.8433162666842335 1104 88-89 0.5406715517784022 1104 90-91 2.2758410793170114 1104 92-93 2.7259736659282794 1104 94-95 2.69813647349293 1104 96-97 2.9559142617194 1104 98-99 2.6588991193467137 1104 100 1.116039983320313 1105 1 5.674835741452391 1105 2 -2.7670587982865413 1105 3 0.540077221295757 1105 4 8.164704528212635 1105 5 8.469705499571432 1105 6 7.956026285690818 1105 7 5.08447814682804 1105 8 5.894961984183087 1105 9 4.873276320918798 1105 10-11 4.833686831605817 1105 12-13 4.9806657292760015 1105 14-15 5.813988950773993 1105 16-17 6.018347650573794 1105 18-19 6.0701135880943475 1105 20-21 6.869523792941383 1105 22-23 2.2601108810654047 1105 24-25 4.057010226626744 1105 26-27 6.765074546389069 1105 28-29 5.935963952730127 1105 30-31 5.229018384485553 1105 32-33 7.026300395319023 1105 34-35 6.086667407369372 1105 36-37 6.153102521118555 1105 38-39 4.058325936678088 1105 40-41 5.220122783653142 1105 42-43 5.575966080874977 1105 44-45 6.161232770240879 1105 46-47 5.375304844335979 1105 48-49 5.038039684652453 1105 50-51 5.89797021952592 1105 52-53 5.699689922333871 1105 54-55 5.335413264466936 1105 56-57 5.147829393225226 1105 58-59 2.039198705200924 1105 60-61 4.467912863697958 1105 62-63 -0.8016029381041818 1105 64-65 -0.3639080194752715 1105 66-67 1.03841003359571 1105 68-69 3.0405228190909845 1105 70-71 3.1717917580500234 1105 72-73 2.869574846687005 1105 74-75 2.8779708218442117 1105 76-77 1.9598699061323934 1105 78-79 3.315824193687174 1105 80-81 1.7424794438143003 1105 82-83 1.1467845618686319 1105 84-85 1.56236963350133 1105 86-87 -0.09467189383599006 1105 88-89 1.6652729514387126 1105 90-91 1.513349513859616 1105 92-93 1.782408064474101 1105 94-95 1.1659318113654606 1105 96-97 2.1928608724231253 1105 98-99 2.0826823560250354 1105 100 2.3091837566048383 1106 1 6.164471102632373 1106 2 10.150685603768562 1106 3 6.737616699309591 1106 4 11.651277170907424 1106 5 9.752465338803251 1106 6 9.319170148449267 1106 7 6.590620307859023 1106 8 7.356907318224479 1106 9 6.814513488731716 1106 10-11 5.628088137385717 1106 12-13 7.831333453685204 1106 14-15 7.718183214855596 1106 16-17 7.703497933633951 1106 18-19 7.080930949406817 1106 20-21 8.044095694999204 1106 22-23 6.13042236126422 1106 24-25 5.092369897097594 1106 26-27 7.5478311711261235 1106 28-29 6.151299981460763 1106 30-31 5.439496638319863 1106 32-33 7.244100225689262 1106 34-35 6.556402656802224 1106 36-37 5.9481172415498165 1106 38-39 7.017404929629002 1106 40-41 6.537556628590689 1106 42-43 6.118400170157571 1106 44-45 4.507230767697521 1106 46-47 5.956420979905122 1106 48-49 5.781982827190319 1106 50-51 6.5493504323558795 1106 52-53 5.682474098331806 1106 54-55 5.248853794684813 1106 56-57 4.898446652238569 1106 58-59 4.995017103622345 1106 60-61 4.933002190815152 1106 62-63 5.002120880324647 1106 64-65 5.217713671414135 1106 66-67 3.1673078637031473 1106 68-69 3.595546824633818 1106 70-71 3.0369473081032936 1106 72-73 3.006048263575483 1106 74-75 3.025618745617244 1106 76-77 3.6370672793688996 1106 78-79 4.200036365545358 1106 80-81 3.789132807250944 1106 82-83 2.1400719148950884 1106 84-85 2.8890412522275 1106 86-87 3.5170113285646956 1106 88-89 3.513419936383798 1106 90-91 3.3487869620720403 1106 92-93 3.123614629428072 1106 94-95 1.723836167114463 1106 96-97 1.863202651407061 1106 98-99 2.2447519911038576 1106 100 3.1129797274094066 1107 1 6.007653337064607 1107 2 10.282131752406656 1107 3 6.05928998455515 1107 4 11.175216462263776 1107 5 9.284850783137752 1107 6 7.854746854523604 1107 7 3.65451556939054 1107 8 6.284102494690984 1107 9 5.58815997849787 1107 10-11 4.430136891356867 1107 12-13 6.084392788702477 1107 14-15 6.2393411298079435 1107 16-17 6.9381021426161205 1107 18-19 6.216667391085199 1107 20-21 5.542296266150014 1107 22-23 5.688499197540107 1107 24-25 4.536143420177986 1107 26-27 7.036420090106038 1107 28-29 6.316860021736442 1107 30-31 5.083139590943862 1107 32-33 5.016851536384888 1107 34-35 6.47994170858772 1107 36-37 6.543364483446943 1107 38-39 6.3643633128729356 1107 40-41 7.679325525175937 1107 42-43 6.181299476617305 1107 44-45 4.036029582429393 1107 46-47 3.509035882774768 1107 48-49 4.023940667927782 1107 50-51 4.820680452059715 1107 52-53 3.911213769008679 1107 54-55 5.236672506383854 1107 56-57 5.148010824243499 1107 58-59 3.0008139479707268 1107 60-61 2.9595298022242815 1107 62-63 0.6215253058753945 1107 64-65 2.156768288795174 1107 66-67 4.244267212608584 1107 68-69 5.049359831334858 1107 70-71 4.421848224722218 1107 72-73 2.52799220529727 1107 74-75 2.5911381955590898 1107 76-77 3.0985324916842316 1107 78-79 3.813358892149875 1107 80-81 2.258913309134524 1107 82-83 1.6200519615072437 1107 84-85 2.292503419996539 1107 86-87 2.3016174905701483 1107 88-89 2.4667553420918367 1107 90-91 1.2958493329555019 1107 92-93 2.1040200553607527 1107 94-95 2.163478123696585 1107 96-97 2.361636923493588 1107 98-99 1.8022427142432775 1107 100 -0.15541848969734318 1108 1 4.34895717090718 1108 2 -3.318392665554141 1108 3 0.12090125121919826 1108 4 6.447939056962589 1108 5 7.043650434807205 1108 6 6.743418561174327 1108 7 4.373225661184588 1108 8 5.016974310740185 1108 9 4.950113119713272 1108 10-11 3.3674617973355474 1108 12-13 2.541443855432444 1108 14-15 4.082641526804085 1108 16-17 4.744320198812392 1108 18-19 4.050773956683258 1108 20-21 4.830156870837772 1108 22-23 4.278181469608231 1108 24-25 2.999202622062139 1108 26-27 4.779160804681862 1108 28-29 6.121494355688309 1108 30-31 4.530084173874666 1108 32-33 4.587422798649552 1108 34-35 3.002067921262771 1108 36-37 1.3890061474932835 1108 38-39 2.7518555510285587 1108 40-41 5.514576628814535 1108 42-43 3.243185834078986 1108 44-45 3.813344066591263 1108 46-47 4.859770637667705 1108 48-49 4.804857589558754 1108 50-51 2.3333384536463875 1108 52-53 0.6332959318162317 1108 54-55 2.7386797900193187 1108 56-57 2.9612679165264453 1108 58-59 3.4915585950132453 1108 60-61 4.675670059829596 1108 62-63 3.903781942963448 1108 64-65 4.125822739706528 1108 66-67 3.696901096020081 1108 68-69 3.930655059916649 1108 70-71 2.504432050917586 1108 72-73 1.9237001359283603 1108 74-75 1.6173344095546476 1108 76-77 2.4786928791437823 1108 78-79 3.0054913470544626 1108 80-81 3.2106303058239547 1108 82-83 2.293474223338862 1108 84-85 0.14849511133216708 1108 86-87 0.43372987845652844 1108 88-89 0.4095587052244447 1108 90-91 0.13671206475925857 1108 92-93 -0.0740697318966177 1108 94-95 0.989830958006678 1108 96-97 1.763017982300525 1108 98-99 -0.511978262492395 1108 100 0.8540626398410751 1109 1 3.1158380637040146 1109 2 7.59650706687308 1109 3 3.7029422173475126 1109 4 -3.641182966259663 1109 5 0.2295087073686588 1109 6 3.839486754454196 1109 7 1.5906709674826054 1109 8 3.4486706026456453 1109 9 2.018036096670496 1109 10-11 1.2274092584878495 1109 12-13 3.794962007472776 1109 14-15 1.9403622776525573 1109 16-17 2.142443434596718 1109 18-19 2.516451799902679 1109 20-21 3.330563151516639 1109 22-23 3.4940489512424726 1109 24-25 3.9012263110153427 1109 26-27 5.337721048021908 1109 28-29 4.690573804714592 1109 30-31 4.072125729658634 1109 32-33 5.650188866716572 1109 34-35 1.0752459398254217 1109 36-37 2.7102993998428353 1109 38-39 2.626739181625684 1109 40-41 3.870678636069808 1109 42-43 0.958186647499705 1109 44-45 2.6163588530000723 1109 46-47 3.3315209095075495 1109 48-49 2.010050089323915 1109 50-51 3.7306233802167448 1109 52-53 2.8574619243782386 1109 54-55 3.438327510428408 1109 56-57 3.9981454571440977 1109 58-59 2.2088025458039695 1109 60-61 0.7047028996171782 1109 62-63 1.541926463201559 1109 64-65 2.823829373068225 1109 66-67 2.4452196354086446 1109 68-69 1.3016679184423232 1109 70-71 0.13462378882384485 1109 72-73 0.31777609957410746 1109 74-75 0.7890523164383687 1109 76-77 -0.04026180681070812 1109 78-79 1.4420758314557922 1109 80-81 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-17.70318677168752 2213 54-55 -17.632007272286234 2213 56-57 -17.475053866251763 2213 58-59 -17.927493583246793 2213 60-61 -19.16711553260196 2213 62-63 -17.50109562482669 2213 64-65 -17.559678956324724 2213 66-67 -8.756631618403343 2213 68-69 -22.598226069139077 2213 70-71 -17.295723476644888 2213 72-73 -9.45126811997308 2213 74-75 -4.624473612805051 2213 76-77 -3.462349773367908 2213 78-79 -4.870376263237944 2213 80-81 -3.5991443873806297 2213 82-83 -3.6206194304194774 2213 84-85 -3.5269401865645307 2213 86-87 -3.6329446557090073 2213 88-89 -3.249673100925122 2213 90-91 -3.170649039807955 2213 92-93 -4.038751551573544 2213 94-95 -3.991245336582111 2213 96-97 -3.6453043202363666 2213 98-99 -4.0342472106448355 2213 100 -3.6596669605936967 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 933.0 3 1304.0 4 10755.0 5 28080.0 6 28449.0 7 34588.0 8 33721.0 9 35111.0 10 39312.0 11 45515.0 12 56598.0 13 74053.0 14 61739.0 15 66021.0 16 71976.0 17 73598.0 18 72558.0 19 71730.0 20 70151.0 21 67912.0 22 69403.0 23 74405.0 24 80377.0 25 86613.0 26 94271.0 27 100662.0 28 107416.0 29 111000.0 30 116208.0 31 124523.0 32 139048.0 33 167371.0 34 203180.0 35 238883.0 36 267297.0 37 243672.0 38 84660.0 39 227.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.21900759558203 21.303990602729147 13.73062724465653 23.746374557032293 2 14.604564651360857 21.57370709342436 40.839647963846495 22.98208029136829 3 17.672342563385122 27.648742584356985 30.030409196824227 24.64850565543367 4 12.242106334151057 18.144032317020322 39.79485324319538 29.819008105633245 5 12.614341290931314 38.68278399701996 35.961982851784235 12.740891860264492 6 30.006115720895405 38.97100812996204 18.556096109374657 12.466780039767903 7 25.52175842561939 33.079200227165686 24.186445894269337 17.212595452945585 8 23.9797129614549 36.94620580757541 21.995729480387908 17.07835175058178 9 24.30246025627519 17.52294664942981 22.568042431814803 35.606550662480196 10-11 22.887932966050283 28.14760056530466 29.331416211241486 19.63305025740357 12-13 23.802740266534027 25.769450535266515 29.346971178037574 21.080838020161888 14-15 21.14790545919066 27.24174381659224 26.822357634825345 24.787993089391755 16-17 20.455649956231074 30.954128562261836 27.83659532078732 20.753626160719776 18-19 20.542301074540887 29.911964782578178 29.460208405206444 20.085525737674494 20-21 21.91781218643366 29.283778281660723 28.422888021574217 20.3755215103314 22-23 20.728755632867994 29.367952036570678 28.863453603239886 21.039838727321445 24-25 21.173887905564033 29.08996773829645 28.411843051828743 21.324301304310772 26-27 21.247316491380243 28.92658787813076 28.464057248183888 21.36203838230511 28-29 20.935134389130404 28.875154762798594 29.049774313096233 21.13993653497477 30-31 21.58649414440612 29.350838283871155 28.290059665658468 20.772607906064252 32-33 20.664084505347923 29.116853397327127 28.980109292171818 21.23895280515314 34-35 20.710641758716545 28.582542383546404 28.960472083204763 21.74634377453229 36-37 21.35192470742419 29.16998637469046 28.43358725420495 21.0445016636804 38-39 21.19623059331891 28.772639498353303 28.71290935080536 21.31822055752243 40-41 21.27395348876951 28.92054537950905 28.373593679107202 21.431907452614237 42-43 21.05342912290591 29.03669730135306 28.799540087087404 21.110333488653627 44-45 21.619624372128538 28.529067167419058 28.594031358481036 21.25727710197136 46-47 21.363428846984 29.045904717241427 28.77140274402546 20.819263691749114 48-49 21.246743083777222 28.748931876994266 28.892410000784164 21.111915038444344 50-51 21.423349802883035 28.800283193879274 28.569987793943056 21.20637920929464 52-53 20.83329192700387 28.91765918317177 28.609693838070243 21.639355051754112 54-55 21.36509335249839 28.9597615880907 28.207681935346823 21.467463124064086 56-57 21.531500890710102 28.766793698970755 28.238980040368382 21.46272536995076 58-59 21.23663386743878 28.304929521061656 28.78266406180881 21.67577254969076 60-61 21.70397212887249 28.817151373228 28.2441929472705 21.23468355062901 62-63 21.04709170422641 28.9532590482173 28.65703266621281 21.342616581343478 64-65 21.000246445989415 28.490858799418906 28.76144676766629 21.74744798692539 66-67 21.31382155462906 28.752448289295184 28.3259705482225 21.607759607853254 68-69 21.823210593587017 28.55494399493077 28.278427079365464 21.343418332116748 70-71 21.7475188777839 28.704381771370365 28.078582618613478 21.46951673223226 72-73 21.475211399531975 28.841917316679204 28.483359862055874 21.199511421732947 74-75 21.850681858729804 28.328281462150006 28.16601599229488 21.65502068682531 76-77 21.647243548440706 28.853914566784695 28.29435523575287 21.20448664902172 78-79 21.720657537729316 28.586145365515677 28.48769028277596 21.20550681397905 80-81 21.707296557782374 28.56845841255827 28.25856807263071 21.465676957028645 82-83 21.201440383354946 28.819405631693417 28.52983439950555 21.449319585446087 84-85 21.451825590898828 28.505775940484725 28.07648655571866 21.965911912897788 86-87 21.744283826408815 28.637835624462447 28.1636926677786 21.45418788135014 88-89 21.57020658591813 28.245920441821614 28.540733488169927 21.64313948409033 90-91 21.87961395384558 28.344616299472918 28.160435975168735 21.615333771512766 92-93 21.295664772231888 28.68164158514166 28.49758810482113 21.52510553780532 94-95 21.217712348603467 28.25032793416656 28.513434302564423 22.01852541466555 96-97 21.59503527142143 28.440735876904483 28.29765128360082 21.66657756807326 98-99 21.623535185279266 28.21820683993793 28.353664609835448 21.80459336494735 100 21.627217860968905 28.151713495285907 28.180535721100135 22.040532922645056 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 433.0 1 500.5 2 475.0 3 395.0 4 534.0 5 844.0 6 1295.0 7 2079.0 8 3151.5 9 4429.5 10 5836.0 11 7271.5 12 8466.0 13 9440.5 14 10494.0 15 11514.5 16 12488.0 17 13590.5 18 14748.5 19 15867.5 20 17128.0 21 18585.0 22 20490.0 23 22776.5 24 25845.0 25 29803.0 26 34800.0 27 41575.5 28 49805.5 29 59060.5 30 68669.0 31 78034.5 32 87647.5 33 96859.5 34 104290.5 35 110891.0 36 116248.0 37 120397.0 38 123919.0 39 124914.5 40 124573.5 41 122710.5 42 118317.0 43 113745.0 44 108813.0 45 103055.5 46 97751.0 47 93321.0 48 90302.5 49 89016.5 50 89483.5 51 84276.5 52 72380.0 53 63034.5 54 56813.0 55 51310.5 56 46257.5 57 41476.5 58 36772.0 59 32361.5 60 27720.0 61 23057.0 62 19029.0 63 15588.5 64 12695.0 65 10176.0 66 7978.0 67 6186.5 68 4828.5 69 3686.5 70 2785.5 71 2126.0 72 1625.0 73 1291.0 74 969.5 75 674.5 76 511.0 77 377.0 78 254.5 79 182.0 80 132.0 81 89.5 82 63.5 83 44.0 84 28.0 85 20.0 86 13.5 87 11.5 88 7.0 89 4.0 90 3.5 91 3.5 92 3.0 93 1.5 94 0.5 95 1.5 96 1.0 97 0.0 98 1.0 99 1.5 100 1.0 >>END_MODULE >>Per base N content fail #Base N-Count 1 9.800788117984089 2 27.38583969606433 3 7.369640859183849 4 26.587639703441408 5 14.842868208476265 6 11.994116779167127 7 9.612795544244033 8 10.921520170164632 9 6.343089520858693 10-11 8.086324124279198 12-13 12.126397034414076 14-15 9.33360382624519 16-17 9.775198873765877 18-19 7.8286335189898315 20-21 9.78492739724343 22-23 7.134296656953512 24-25 6.405103094684813 26-27 11.407808023803376 28-29 7.682413657433022 30-31 5.131834556699003 32-33 10.569848646920683 34-35 8.65180799921311 36-37 8.623298661060087 38-39 7.652936077606876 40-41 10.065640637871466 42-43 8.009648605117233 44-45 8.137856097770893 46-47 7.255280759347374 48-49 4.9445643219849265 50-51 7.958178107287325 52-53 5.6601717629990285 54-55 6.378069172414641 56-57 6.100045491989721 58-59 4.630823282062631 60-61 5.269109709466022 62-63 3.6026274697847125 64-65 5.209447579703196 66-67 3.500516395558998 68-69 6.474893339726802 70-71 3.397713720138197 72-73 2.0447573555629326 74-75 1.1936882938044828 76-77 1.51204000836069 78-79 2.3121303775835145 80-81 2.7944069442907553 82-83 1.280999102455338 84-85 1.784008336099738 86-87 0.6118979995819656 88-89 0.7476823675506867 90-91 0.34171246603469685 92-93 0.5819900901233201 94-95 0.7849827253390383 96-97 0.8163199439341965 98-99 0.4736853429727171 100 0.9256083016733675 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 3253320.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 95.74522925690913 #Duplication Level Percentage of deduplicated Percentage of total 1 97.52566025674253 93.37616699713244 2 2.0549342574443816 3.935003031737773 3 0.2465184530802488 0.7080889741868105 4 0.06639194601695264 0.25426848368821864 5 0.030164436166705957 0.14440504280933314 6 0.018819740244247506 0.10811402065445735 7 0.012974715082795433 0.08695869491017279 8 0.008501466377481042 0.06511798778654616 9 0.005201622290403608 0.0448227466832288 >10 0.02787045134334685 0.44854950728089366 >50 0.0014013211457565597 0.09183544468196561 >100 0.0012055586231518062 0.2715433482624345 >500 1.9405933335655184E-4 0.14189121605661167 >1k 1.6171610863927E-4 0.3232345041291156 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 3694 0.11354554731781688 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 6.147566178549913E-5 0.0 6 0.0 0.0 0.0 6.147566178549913E-5 3.073783089274956E-5 7 0.0 3.073783089274956E-5 0.0 6.147566178549913E-5 3.073783089274956E-5 8 0.0 3.073783089274956E-5 0.0 6.147566178549913E-5 3.073783089274956E-5 9 0.0 3.073783089274956E-5 0.0 6.147566178549913E-5 3.073783089274956E-5 10-11 0.0 3.073783089274956E-5 0.0 1.0758240812462347E-4 3.073783089274956E-5 12-13 0.0 3.073783089274956E-5 0.0 1.3832023901737302E-4 3.073783089274956E-5 14-15 0.0 3.073783089274956E-5 0.0 1.9979590080287213E-4 3.073783089274956E-5 16-17 3.073783089274956E-5 3.073783089274956E-5 0.0 2.1516481624924693E-4 3.073783089274956E-5 18-19 3.073783089274956E-5 3.073783089274956E-5 0.0 2.459026471419965E-4 3.073783089274956E-5 20-21 3.073783089274956E-5 3.073783089274956E-5 0.0 2.459026471419965E-4 3.073783089274956E-5 22-23 6.147566178549913E-5 3.073783089274956E-5 0.0 2.612715625883713E-4 3.073783089274956E-5 24-25 6.147566178549913E-5 3.073783089274956E-5 0.0 2.7664047803474605E-4 3.073783089274956E-5 26-27 6.147566178549913E-5 3.073783089274956E-5 0.0 3.8422288615936955E-4 3.073783089274956E-5 28-29 6.147566178549913E-5 3.073783089274956E-5 0.0 8.299214341042381E-4 3.073783089274956E-5 30-31 6.147566178549913E-5 3.073783089274956E-5 0.0 0.0025512399640982134 3.073783089274956E-5 32-33 6.147566178549913E-5 3.073783089274956E-5 0.0 0.007131176767117898 3.073783089274956E-5 34-35 6.147566178549913E-5 4.610674633912434E-5 0.0 0.013493907761917057 3.073783089274956E-5 36-37 6.147566178549913E-5 6.147566178549913E-5 0.0 0.023330013647596917 3.073783089274956E-5 38-39 6.147566178549913E-5 6.147566178549913E-5 0.0 0.04014360714593093 3.073783089274956E-5 40-41 6.147566178549913E-5 6.147566178549913E-5 0.0 0.05906274206041828 3.073783089274956E-5 42-43 6.147566178549913E-5 6.147566178549913E-5 0.0 0.07914991454883011 3.073783089274956E-5 44-45 6.147566178549913E-5 6.147566178549913E-5 0.0 0.10303320915249653 3.073783089274956E-5 46-47 6.147566178549913E-5 6.147566178549913E-5 0.0 0.13249542006319698 3.073783089274956E-5 48-49 6.147566178549913E-5 6.147566178549913E-5 0.0 0.1635713670957668 3.073783089274956E-5 50-51 6.147566178549913E-5 6.147566178549913E-5 0.0 0.1990274550305534 3.073783089274956E-5 52-53 6.147566178549913E-5 6.147566178549913E-5 0.0 0.23598969667908476 3.073783089274956E-5 54-55 6.147566178549913E-5 6.147566178549913E-5 0.0 0.27634846864126494 3.073783089274956E-5 56-57 6.147566178549913E-5 6.147566178549913E-5 0.0 0.3270351517834089 3.073783089274956E-5 58-59 6.147566178549913E-5 6.147566178549913E-5 0.0 0.3800886479042947 3.073783089274956E-5 60-61 6.147566178549913E-5 6.147566178549913E-5 0.0 0.43606223795999166 3.073783089274956E-5 62-63 6.147566178549913E-5 6.147566178549913E-5 0.0 0.4974764240837053 3.073783089274956E-5 64-65 9.221349267824868E-5 6.147566178549913E-5 0.0 0.5678967946589946 3.073783089274956E-5 66-67 9.221349267824868E-5 6.147566178549913E-5 0.0 0.6502434436206705 3.073783089274956E-5 68-69 9.221349267824868E-5 6.147566178549913E-5 0.0 0.746545067807655 3.073783089274956E-5 70-71 1.2295132357099825E-4 6.147566178549913E-5 0.0 0.8612125459530572 4.610674633912434E-5 72-73 1.2295132357099825E-4 6.147566178549913E-5 0.0 0.9796915151291603 6.147566178549913E-5 74-75 1.3832023901737302E-4 7.684457723187391E-5 0.0 1.1043026815683672 6.147566178549913E-5 76-77 1.536891544637478E-4 9.221349267824868E-5 0.0 1.2423155422768126 6.147566178549913E-5 78-79 1.536891544637478E-4 9.221349267824868E-5 0.0 1.395543629277169 6.147566178549913E-5 80-81 1.690580699101226E-4 9.221349267824868E-5 0.0 1.570687789704056 6.147566178549913E-5 82-83 1.8442698535649736E-4 9.221349267824868E-5 0.0 1.7680400329509547 6.147566178549913E-5 84-85 1.8442698535649736E-4 9.221349267824868E-5 0.0 1.9635172685133955 6.147566178549913E-5 86-87 1.8442698535649736E-4 9.221349267824868E-5 0.0 2.175731252996939 6.147566178549913E-5 88 1.8442698535649736E-4 9.221349267824868E-5 0.0 2.3611572178574503 6.147566178549913E-5 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTTACGC 185 3.792331E-5 21.535824 3 CGACCGA 120 0.003284589 21.104994 6 GTGCGCG 185 9.220195E-5 19.187744 9 GTGTATA 1955 0.0 18.059534 1 ACCGATC 240 3.5251178E-5 16.897411 8 GTATATA 2655 0.0 16.860058 1 GTATAGG 815 0.0 16.24527 1 GTGTAGA 1335 0.0 16.056946 1 CTATACT 1350 0.0 16.022192 4 GTCTAGG 990 0.0 15.920989 1 GTATTAG 1360 0.0 15.761779 1 GTACATG 14060 0.0 15.694517 1 CATGGGG 4135 0.0 15.279865 4 TACATGG 14305 0.0 15.112938 2 GTATAGA 1215 0.0 15.048285 1 GTCTATA 1015 0.0 14.907692 1 ACATGGG 14090 0.0 14.50169 3 GTCTAGA 965 0.0 14.373437 1 GTACATA 1610 0.0 13.705895 1 TAATCCG 340 4.5846187E-5 13.399774 5 >>END_MODULE