FastQCFastQC Report
Wed 25 May 2016
SRR1295086_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295086_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3253320
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT109310.33599522948864546No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA81040.24909938155484243No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG34540.10616846790355698No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGTACT45400.015.50871312-13
GTACTAG8200.015.361591
CTTACAC17350.015.1692293
TATACTG20650.014.7945485
AGTACTT53850.014.77489312-13
GTATAGG8650.014.5624331
CTGTCGC5209.276846E-1114.4432089
CATGGGG45150.014.2616984
CCTACAC11900.014.2177373
GTATAAA22500.013.6602091
TGCACCG3456.986382E-613.6235195
GTATCAA167800.013.5423681
CTACACT14950.013.2043334
CCGTAGC2852.9341795E-413.1762619
GTACATG196750.012.8301871
TACATCG2953.883894E-412.7460725
CATGGGT24950.012.6215494
GTCCTAG11200.012.5965041
ACATGGG188850.012.5923993
GTACTTT56500.012.58619714-15
Produced by FastQC (version 0.11.5)