##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1295086_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3253320 Sequences flagged as poor quality 0 Sequence length 100 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.962725769367907 33.0 33.0 33.0 27.0 33.0 2 29.385352808822987 33.0 33.0 33.0 15.0 33.0 3 30.37641916565232 33.0 33.0 33.0 22.0 33.0 4 34.723957987532735 37.0 33.0 37.0 27.0 37.0 5 35.088219726310356 37.0 37.0 37.0 33.0 37.0 6 34.7315314816864 37.0 37.0 37.0 27.0 37.0 7 34.622578781060575 37.0 37.0 37.0 27.0 37.0 8 34.60281742957963 37.0 37.0 37.0 27.0 37.0 9 34.570117602941 37.0 37.0 37.0 27.0 37.0 10-11 34.54554562723618 37.0 37.0 37.0 27.0 37.0 12-13 34.4338657125644 37.0 37.0 37.0 27.0 37.0 14-15 35.77988531715294 37.0 37.0 40.0 27.0 40.0 16-17 35.77388682945421 37.0 37.0 40.0 27.0 40.0 18-19 35.810549838319005 37.0 37.0 40.0 27.0 40.0 20-21 35.8084336923512 37.0 37.0 40.0 27.0 40.0 22-23 35.774661576481876 37.0 37.0 40.0 27.0 40.0 24-25 35.73845118217697 37.0 37.0 40.0 27.0 40.0 26-27 35.70936812241034 37.0 37.0 40.0 27.0 40.0 28-29 35.65480048073968 37.0 37.0 40.0 27.0 40.0 30-31 35.56164026286993 37.0 37.0 40.0 27.0 40.0 32-33 35.51331885581498 37.0 37.0 40.0 27.0 40.0 34-35 35.391523274685554 37.0 33.0 40.0 27.0 40.0 36-37 35.38596372321198 37.0 35.0 40.0 27.0 40.0 38-39 35.32411121561974 37.0 33.0 40.0 27.0 40.0 40-41 35.26343366161336 37.0 33.0 40.0 27.0 40.0 42-43 35.166152576444986 37.0 33.0 40.0 27.0 40.0 44-45 35.05637179865491 37.0 33.0 40.0 27.0 40.0 46-47 34.95139672703577 37.0 33.0 40.0 22.0 40.0 48-49 34.85226061377301 37.0 33.0 40.0 22.0 40.0 50-51 34.760384776167115 37.0 33.0 40.0 22.0 40.0 52-53 34.67064660101066 37.0 33.0 40.0 22.0 40.0 54-55 34.54568348640773 37.0 33.0 40.0 22.0 40.0 56-57 34.418420106229945 37.0 33.0 40.0 22.0 40.0 58-59 34.296174984323706 37.0 33.0 40.0 22.0 40.0 60-61 34.13785748097328 37.0 33.0 40.0 22.0 40.0 62-63 33.96016331009554 37.0 33.0 40.0 22.0 40.0 64-65 33.79093741162873 37.0 33.0 40.0 22.0 40.0 66-67 33.57123061979762 37.0 33.0 40.0 22.0 40.0 68-69 33.36383048701019 37.0 33.0 37.0 22.0 40.0 70-71 33.13795814736946 37.0 33.0 37.0 22.0 40.0 72-73 32.92949586883553 37.0 33.0 37.0 22.0 40.0 74-75 32.69310519715245 37.0 33.0 37.0 22.0 40.0 76-77 32.45524986782733 37.0 33.0 37.0 22.0 38.5 78-79 32.22209481391317 37.0 33.0 37.0 22.0 37.0 80-81 32.011663162553944 37.0 33.0 37.0 22.0 37.0 82-83 31.810151014963175 33.0 33.0 37.0 22.0 37.0 84-85 31.636199021307462 33.0 33.0 37.0 22.0 37.0 86-87 31.6404437620646 33.0 33.0 37.0 22.0 37.0 88-89 31.652520963200672 33.0 33.0 37.0 22.0 37.0 90-91 31.653977321628368 33.0 33.0 37.0 22.0 37.0 92-93 31.612710093074153 33.0 33.0 37.0 22.0 37.0 94-95 31.632048030934556 33.0 33.0 37.0 22.0 37.0 96-97 31.584487077815893 33.0 33.0 37.0 22.0 37.0 98-99 31.508383435997686 33.0 33.0 37.0 22.0 37.0 100 30.968072307673392 33.0 27.0 37.0 15.0 37.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.8121649003879874 1101 2 4.319299798948673 1101 3 2.4623251548977514 1101 4 1.4104580403590177 1101 5 0.6483983773422253 1101 6 0.6769161931346375 1101 7 0.7171709089483684 1101 8 0.6973877429126958 1101 9 0.8062809028553204 1101 10-11 0.8729461031637058 1101 12-13 0.9146198587774776 1101 14-15 1.1972033086335188 1101 16-17 1.2398845960164877 1101 18-19 1.3228130403665546 1101 20-21 1.2605364546578173 1101 22-23 1.1871710125202526 1101 24-25 1.192624520764646 1101 26-27 1.253049310544263 1101 28-29 1.1387017407527082 1101 30-31 1.0226187194283867 1101 32-33 1.2790382802395328 1101 34-35 1.2970274451734696 1101 36-37 1.2640805424687116 1101 38-39 1.2874766695762574 1101 40-41 1.2813527756029472 1101 42-43 1.3531029995589066 1101 44-45 1.3703966219818469 1101 46-47 1.3858988604637332 1101 48-49 1.3393074777293918 1101 50-51 1.2527945661737974 1101 52-53 1.2834063677551413 1101 54-55 1.3351536412275138 1101 56-57 1.3207792921444295 1101 58-59 1.2932271506595043 1101 60-61 1.305861028541429 1101 62-63 1.2599558288463726 1101 64-65 1.4074189096280634 1101 66-67 1.2748773597938765 1101 68-69 1.263934163575854 1101 70-71 1.232708386767385 1101 72-73 1.2327870132147254 1101 74-75 1.1194304543960456 1101 76-77 1.2281245041323032 1101 78-79 1.1515694080388457 1101 80-81 1.1550701418940115 1101 82-83 1.1785885667882212 1101 84-85 1.1342106696795078 1101 86-87 1.1945804269190248 1101 88-89 1.108262323649818 1101 90-91 1.2343478772726435 1101 92-93 1.2355046777996321 1101 94-95 1.1751744462379001 1101 96-97 1.1954482157962936 1101 98-99 1.182305393270827 1101 100 1.2370402384429937 1102 1 0.6432989933554758 1102 2 4.164147816127667 1102 3 2.40355436786923 1102 4 1.187682632074825 1102 5 0.6116992515153754 1102 6 0.6282651147235612 1102 7 0.6351672460697273 1102 8 0.6293148561024111 1102 9 0.6965453251075076 1102 10-11 0.7321854104527787 1102 12-13 0.7630411931289558 1102 14-15 1.0980989032261377 1102 16-17 1.1025651133970342 1102 18-19 1.064704938933616 1102 20-21 1.0320984681758034 1102 22-23 0.9930570630160744 1102 24-25 1.0685508824158347 1102 26-27 0.9889357962189749 1102 28-29 1.0019851831836206 1102 30-31 0.998556366227227 1102 32-33 1.1779598456917881 1102 34-35 1.2823674930020204 1102 36-37 1.0965156236454447 1102 38-39 1.1513376348584856 1102 40-41 1.1200540305654485 1102 42-43 1.1892115426973078 1102 44-45 1.2011599858910174 1102 46-47 1.1662602717316801 1102 48-49 1.0873479713156868 1102 50-51 1.0771258429459891 1102 52-53 1.1129942010383118 1102 54-55 1.088170551894983 1102 56-57 1.072678282540565 1102 58-59 1.0694318323484922 1102 60-61 1.0501872356024151 1102 62-63 1.1124295571276832 1102 64-65 1.0926510299611252 1102 66-67 1.0880893016839295 1102 68-69 1.0875175562461763 1102 70-71 1.0568031425800584 1102 72-73 1.0397408612969627 1102 74-75 1.0588049492158191 1102 76-77 1.0152835572804761 1102 78-79 0.9104091553883293 1102 80-81 0.8942188488259681 1102 82-83 0.9711582548100921 1102 84-85 1.0055009009012004 1102 86-87 1.0875900300678367 1102 88-89 0.9595915671955808 1102 90-91 0.9660947320769573 1102 92-93 1.0099187447305908 1102 94-95 0.9834999013133263 1102 96-97 1.0086497420024365 1102 98-99 1.023751326226769 1102 100 1.085268636778352 1103 1 0.6220347437255 1103 2 4.096067483345422 1103 3 2.3992075260805983 1103 4 1.2274335640112284 1103 5 0.5897335805076409 1103 6 0.5832085942353871 1103 7 0.5325916922519269 1103 8 0.5921658599239592 1103 9 0.6260869873771853 1103 10-11 0.5932880132002865 1103 12-13 0.7618945370511412 1103 14-15 0.8666250231507746 1103 16-17 0.905731633948335 1103 18-19 0.9567835471938224 1103 20-21 0.9549475508509389 1103 22-23 0.9059530063305345 1103 24-25 0.9388703767304136 1103 26-27 0.8760335505521581 1103 28-29 0.8569607043509535 1103 30-31 0.8692201448606554 1103 32-33 0.9174349510617077 1103 34-35 1.0181459812131237 1103 36-37 0.9269190959597395 1103 38-39 0.9028280228128054 1103 40-41 0.8805004107377741 1103 42-43 0.8810125092233605 1103 44-45 0.9542354549258434 1103 46-47 0.9750700358455688 1103 48-49 0.971120665421374 1103 50-51 0.8338542749207178 1103 52-53 0.9039413735707882 1103 54-55 0.9385585468044013 1103 56-57 0.8828789724395776 1103 58-59 0.8706989321299545 1103 60-61 0.8196549202060908 1103 62-63 0.9144645292079332 1103 64-65 0.8866099377217438 1103 66-67 0.8389401305278241 1103 68-69 0.8104243409596208 1103 70-71 0.8809826980646633 1103 72-73 0.8298096617890707 1103 74-75 0.8216481624729894 1103 76-77 0.7310601153256968 1103 78-79 0.6940698389917515 1103 80-81 0.7986761114358636 1103 82-83 0.7494229071592571 1103 84-85 0.8275007218647907 1103 86-87 0.7727776642887747 1103 88-89 0.7960288147068795 1103 90-91 0.7935993365147134 1103 92-93 0.7256427982052074 1103 94-95 0.808037304139841 1103 96-97 0.8089749388748437 1103 98-99 0.7732608025294745 1103 100 0.6907017831381417 1104 1 0.41841027500163364 1104 2 3.6298187327399987 1104 3 2.21170328584855 1104 4 1.0684695320630055 1104 5 0.47930047821782296 1104 6 0.33857895016092243 1104 7 0.38221202142091215 1104 8 0.3780190399619414 1104 9 0.4213865881151193 1104 10-11 0.43667769551394287 1104 12-13 0.5525503127460425 1104 14-15 0.683888073541965 1104 16-17 0.6811660649953666 1104 18-19 0.5725253746567489 1104 20-21 0.6732959645960008 1104 22-23 0.5683858201938676 1104 24-25 0.6064471362679882 1104 26-27 0.6623622488054508 1104 28-29 0.6182646187880607 1104 30-31 0.5789338589048398 1104 32-33 0.6721756892158126 1104 34-35 0.7605909761067124 1104 36-37 0.6928703047206497 1104 38-39 0.6272028039986921 1104 40-41 0.6194872129588447 1104 42-43 0.6337810859747393 1104 44-45 0.778508561242738 1104 46-47 0.6503433607498366 1104 48-49 0.5600345220462017 1104 50-51 0.6159333763361872 1104 52-53 0.6621201009388287 1104 54-55 0.5927759677637496 1104 56-57 0.6201483673063066 1104 58-59 0.7562149343575442 1104 60-61 0.6931219969583751 1104 62-63 0.7456154959808359 1104 64-65 0.728107456165624 1104 66-67 0.6855032750657628 1104 68-69 0.6581109429147531 1104 70-71 0.5859878725082623 1104 72-73 0.5840422750236769 1104 74-75 0.5825991852985908 1104 76-77 0.5440060652918746 1104 78-79 0.6033012776594049 1104 80-81 0.5580359588419235 1104 82-83 0.5924134861346033 1104 84-85 0.5516196832755043 1104 86-87 0.6241163758387103 1104 88-89 0.5805662530081079 1104 90-91 0.6045799729395362 1104 92-93 0.657912120511817 1104 94-95 0.6304429397030056 1104 96-97 0.5415762917582931 1104 98-99 0.6245051178975238 1104 100 0.5800305476025471 1105 1 0.5882895747098402 1105 2 3.825627071551164 1105 3 2.205131639904536 1105 4 1.1102505715614939 1105 5 0.42864521765433494 1105 6 0.3433569900024054 1105 7 0.4106262250245081 1105 8 0.3457950231806066 1105 9 0.364356416499966 1105 10-11 0.3990401910266712 1105 12-13 0.48069651950781633 1105 14-15 0.6208123792420253 1105 16-17 0.5976219934605638 1105 18-19 0.6212410833518689 1105 20-21 0.5436052340022783 1105 22-23 0.5949801699102792 1105 24-25 0.5418686271906523 1105 26-27 0.554358452778537 1105 28-29 0.5026605735127205 1105 30-31 0.4207854104161086 1105 32-33 0.5721273968845608 1105 34-35 0.6548955032186257 1105 36-37 0.5493573649894259 1105 38-39 0.46788030040999473 1105 40-41 0.5237283115918672 1105 42-43 0.583085993443774 1105 44-45 0.6015950124192173 1105 46-47 0.49289599081378555 1105 48-49 0.5129619605956321 1105 50-51 0.44345029189950935 1105 52-53 0.40607989966120783 1105 54-55 0.3963959257080205 1105 56-57 0.3791220111176301 1105 58-59 0.368270791080306 1105 60-61 0.3934772873307253 1105 62-63 0.3838840654689335 1105 64-65 0.3669945213561334 1105 66-67 0.39046230112880664 1105 68-69 0.4295354220882004 1105 70-71 0.3979342160189674 1105 72-73 0.3863801555480677 1105 74-75 0.4824410519386504 1105 76-77 0.40041676224532097 1105 78-79 0.42564062123396695 1105 80-81 0.41936608340416726 1105 82-83 0.4238834213517464 1105 84-85 0.3984206149573488 1105 86-87 0.5010734075191792 1105 88-89 0.3874598867460257 1105 90-91 0.4414358233394644 1105 92-93 0.45051380182500367 1105 94-95 0.4721032798078362 1105 96-97 0.325771496959387 1105 98-99 0.2905865719807892 1105 100 0.24610973391314062 1106 1 0.43569994080387175 1106 2 3.887458986054291 1106 3 2.155253236856847 1106 4 0.9919093377384982 1106 5 0.44847971461305747 1106 6 0.3096451716300521 1106 7 0.2024929885604081 1106 8 0.15447563597624736 1106 9 0.17199220989917308 1106 10-11 0.1635613127550286 1106 12-13 0.19942930268470604 1106 14-15 0.36425394113896914 1106 16-17 0.3043638749608206 1106 18-19 0.30232784321717077 1106 20-21 0.2586955493594232 1106 22-23 0.2164107721176407 1106 24-25 0.3421810630500772 1106 26-27 0.3299997390542728 1106 28-29 0.29952645197658256 1106 30-31 0.30015497849009165 1106 32-33 0.3504230956516494 1106 34-35 0.49966064419485434 1106 36-37 0.39279975222961383 1106 38-39 0.26085707459934326 1106 40-41 0.3109837846492951 1106 42-43 0.3235271502709338 1106 44-45 0.3544601154948097 1106 46-47 0.28752161620133876 1106 48-49 0.20843757007360608 1106 50-51 0.15784036866729423 1106 52-53 0.19384667286231405 1106 54-55 0.20327669292972672 1106 56-57 0.16348737102701705 1106 58-59 0.19934998167920526 1106 60-61 0.20920165092049103 1106 62-63 0.4070455210703585 1106 64-65 0.28282547368279154 1106 66-67 0.18895998093689315 1106 68-69 0.17484078280121196 1106 70-71 0.2874494897560069 1106 72-73 0.3247071196206619 1106 74-75 0.15822221217577948 1106 76-77 0.3029206799108195 1106 78-79 0.2067040760122154 1106 80-81 0.2028226535165345 1106 82-83 0.27875144788001904 1106 84-85 0.19879434341290292 1106 86-87 0.38565822185496046 1106 88-89 0.2904002915334516 1106 90-91 0.3817327948727218 1106 92-93 0.43664195374028836 1106 94-95 0.34548411381718935 1106 96-97 0.24179742576918883 1106 98-99 0.3720798676958452 1106 100 0.4193473491489783 1107 1 0.4362885820204738 1107 2 3.520740412368891 1107 3 2.0683179426881395 1107 4 1.0070517565361286 1107 5 0.27392917037057174 1107 6 0.2694501514054082 1107 7 0.1832898220586614 1107 8 0.22455475511934964 1107 9 0.24300031711580772 1107 10-11 0.16423332636542654 1107 12-13 0.18463067023500912 1107 14-15 0.21105461775303525 1107 16-17 0.13092966797507444 1107 18-19 0.2457574063475505 1107 20-21 0.2634893535015621 1107 22-23 0.32051875043474354 1107 24-25 0.2600333233490204 1107 26-27 0.17054908110242906 1107 28-29 0.09553911624753653 1107 30-31 0.17396905935393647 1107 32-33 0.26316635555130574 1107 34-35 0.36014764139986255 1107 36-37 0.18163315717380613 1107 38-39 0.13779694752263083 1107 40-41 0.15071105579836797 1107 42-43 0.25034082533993995 1107 44-45 0.36535338494258696 1107 46-47 0.2576603351048732 1107 48-49 0.14964199953040946 1107 50-51 0.18786408705001634 1107 52-53 0.22976561371608994 1107 54-55 0.23553925954951893 1107 56-57 0.21083387424733502 1107 58-59 0.19707949446500095 1107 60-61 0.28747008963027554 1107 62-63 0.35918057697021766 1107 64-65 0.28642538384372074 1107 66-67 0.24549068463287682 1107 68-69 0.1756195725343872 1107 70-71 0.184552809027565 1107 72-73 0.1626873897454857 1107 74-75 0.16205916511226093 1107 76-77 0.18917702723647523 1107 78-79 0.21605817866311838 1107 80-81 0.30655383939226866 1107 82-83 0.25993733446578915 1107 84-85 0.3070099202925789 1107 86-87 0.36264325301653244 1107 88-89 0.2466566171588518 1107 90-91 0.21904444747762497 1107 92-93 0.23831591169386712 1107 94-95 0.20097769705070334 1107 96-97 0.16262389097065366 1107 98-99 0.28418087588986296 1107 100 0.2624163516314866 1108 1 0.4337850101067495 1108 2 3.7721198744166173 1108 3 2.118917647522565 1108 4 1.0751762061821424 1108 5 0.24549789212883155 1108 6 0.1223130566331605 1108 7 0.11856853821081614 1108 8 -0.08075366549486773 1108 9 0.1882969411056905 1108 10-11 0.11547101515795788 1108 12-13 0.10670529745961232 1108 14-15 0.09215144738654146 1108 16-17 0.21055094556517417 1108 18-19 0.1273215196992723 1108 20-21 0.17779001520202797 1108 22-23 0.04179463603489353 1108 24-25 0.10763562885384204 1108 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100 -0.10253891992175923 2212 1 0.6743409053021949 2212 2 4.276456023412482 2212 3 2.4520084492721494 2212 4 1.1974673204740185 2212 5 0.6613025063888074 2212 6 0.6350858715472327 2212 7 0.59618923161932 2212 8 0.4284158939984479 2212 9 0.33572302637657714 2212 10-11 0.4312579004364423 2212 12-13 0.44051472459714347 2212 14-15 0.6148631447830724 2212 16-17 0.567465866545362 2212 18-19 0.535908832283738 2212 20-21 0.5398348324334492 2212 22-23 0.5970668333048081 2212 24-25 0.4948657834889758 2212 26-27 0.41073217303404874 2212 28-29 0.5032654393422717 2212 30-31 0.4362412619587772 2212 32-33 0.5954170369657703 2212 34-35 0.683435205495968 2212 36-37 0.6076229386537193 2212 38-39 0.5936834515607714 2212 40-41 0.5982996756374206 2212 42-43 0.576193694652197 2212 44-45 0.5139152228558359 2212 46-47 0.6625226723025506 2212 48-49 0.5516259674122352 2212 50-51 0.5856611316170373 2212 52-53 0.46439940288393444 2212 54-55 0.47260373412323275 2212 56-57 0.5831537264217488 2212 58-59 0.5622350556340763 2212 60-61 0.5971989217326268 2212 62-63 0.6771688625745753 2212 64-65 0.6027415970131784 2212 66-67 0.599727433009356 2212 68-69 0.7005043478653832 2212 70-71 0.7335234057237017 2212 72-73 0.5627401589131864 2212 74-75 0.4629692759365085 2212 76-77 0.5622616727571241 2212 78-79 0.4462956983504185 2212 80-81 0.5706050911092895 2212 82-83 0.40143477871205846 2212 84-85 0.5465359821484732 2212 86-87 0.4978777297117958 2212 88-89 0.6581420692297044 2212 90-91 0.6251612884833158 2212 92-93 0.679157809154475 2212 94-95 0.5602502151917896 2212 96-97 0.3852386481785466 2212 98-99 0.4091803140375667 2212 100 0.43538752546229276 2213 1 -1.0161079119001108 2213 2 -25.440160061564757 2213 3 -14.536941357415142 2213 4 -6.484119399308572 2213 5 -2.720910406467489 2213 6 -2.0474496412285106 2213 7 -1.872102178662054 2213 8 -1.6662947138368693 2213 9 -1.7641649933863377 2213 10-11 -1.745810978226224 2213 12-13 -1.7513053869298147 2213 14-15 -2.0496028063226674 2213 16-17 -2.1154912461570348 2213 18-19 -2.0298116940954856 2213 20-21 -1.9963237354969365 2213 22-23 -2.065841490216087 2213 24-25 -2.0808123047498057 2213 26-27 -2.199582782972243 2213 28-29 -2.1492684869609207 2213 30-31 -2.1733982842688775 2213 32-33 -2.042275515987896 2213 34-35 -2.032322226973349 2213 36-37 -2.0720142999775035 2213 38-39 -2.0322534943971746 2213 40-41 -2.151846351948663 2213 42-43 -2.1955729146336083 2213 44-45 -2.279702727771898 2213 46-47 -2.0630352200377686 2213 48-49 -2.2453412173304272 2213 50-51 -2.103414974767283 2213 52-53 -2.2386121649733113 2213 54-55 -2.1559350525549306 2213 56-57 -2.113992915149808 2213 58-59 -2.142884898172781 2213 60-61 -2.1852579627855633 2213 62-63 -2.3152997711120022 2213 64-65 -2.073393426222463 2213 66-67 -2.0192600678534234 2213 68-69 -2.186537133240652 2213 70-71 -2.041600292776465 2213 72-73 -2.2388456955207516 2213 74-75 -2.2461005051611878 2213 76-77 -2.212865373818211 2213 78-79 -2.0870614320710104 2213 80-81 -2.106011153545378 2213 82-83 -2.153276632661303 2213 84-85 -2.1269222549920848 2213 86-87 -2.1313359516565242 2213 88-89 -2.194687105794273 2213 90-91 -2.0952260168518713 2213 92-93 -2.101670751884029 2213 94-95 -2.19090010617192 2213 96-97 -2.1145421816702132 2213 98-99 -2.220400754194145 2213 100 -2.240237560171167 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4.0 3 11.0 4 38.0 5 37.0 6 38.0 7 60.0 8 66.0 9 101.0 10 544.0 11 3118.0 12 9266.0 13 17376.0 14 22405.0 15 25079.0 16 25423.0 17 25860.0 18 26129.0 19 27265.0 20 28737.0 21 31352.0 22 33924.0 23 35512.0 24 36776.0 25 38233.0 26 41587.0 27 46675.0 28 53898.0 29 62651.0 30 74181.0 31 90207.0 32 111654.0 33 146139.0 34 196040.0 35 272423.0 36 403611.0 37 669428.0 38 692220.0 39 5252.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 37.97701741377652 20.96552922306573 10.234076151533673 30.823377211624077 2 12.356071486662596 22.778850073326577 36.58242177236296 28.282656667647863 3 14.996311460292869 27.327652982184354 28.92666568305608 28.749369874466694 4 10.042945948845414 18.64762835139629 37.948160541799524 33.36126515795877 5 11.070093204725264 38.89391435214834 35.54523163246204 14.490760810664355 6 29.360292742697425 40.099727629714195 17.76284224667349 12.777137380914889 7 25.383955699228782 33.504902782266285 23.56327370375074 17.547867814754195 8 24.08077150278649 37.3074956203053 21.08345205737254 17.52828081953567 9 24.30915441699905 16.858123618347275 22.218990016276173 36.613731948377506 10-11 22.50100786581224 28.157018574559466 29.30354089167603 20.038432667952264 12-13 23.396947486227987 25.58833853453078 29.677145476649503 21.337568502591733 14-15 21.10814907387732 27.013851325275045 26.732599585902477 25.145400014945153 16-17 20.190154854042266 30.745790953576996 27.85747702741367 21.20657716496707 18-19 20.294443472504536 29.65317938625121 29.839120543676877 20.213256597567376 20-21 21.352664163816858 28.951327247253623 29.059826432049352 20.636182156880167 22-23 20.48239314667902 28.990059966712316 29.216220665941183 21.31132622066748 24-25 20.763129513212522 28.803180892509296 28.969167502902227 21.46452209137595 26-27 20.701924274074177 28.681367327458602 29.079733100926404 21.536975297540824 28-29 20.37730431128067 28.59593384231272 29.651687407171927 21.37507443923468 30-31 21.157826302380105 29.03143932423647 28.87460320168907 20.936131171694356 32-33 20.068524615207988 29.026043995243572 29.577225593773182 21.328205795775254 34-35 20.18004900059956 28.640252573945457 29.71878217479852 21.460916250656464 36-37 20.95641141306144 28.98224134525902 28.972297297816535 21.08904994386301 38-39 20.754035490677303 28.906676511526108 29.226552471215467 21.11273552658112 40-41 20.76213569029008 28.8078696107723 29.056077370611582 21.37391732832604 42-43 20.617236626278473 28.82899859088081 29.26622823304323 21.287536549797483 44-45 21.08916320863729 28.389742325449568 29.054332586241188 21.466761879671953 46-47 20.88402978994858 29.01193596237059 29.125454419687806 20.97857982799302 48-49 20.856567516218004 28.843069514768953 29.117951939157837 21.182411029855206 50-51 20.953188514317137 28.799108771367788 28.76821030388566 21.479492410429415 52-53 20.44923959393766 29.00614836949988 28.75367381757572 21.79093821898674 54-55 20.931717637061112 28.950511743395634 28.43904505721583 21.678725562327422 56-57 21.110288911713717 28.624093202364858 28.553615308444158 21.712002577477264 58-59 20.639047164209572 28.34417789768599 29.207172706713187 21.80960223139125 60-61 21.20363984412262 28.821422088285324 28.42827022865305 21.546667838939005 62-63 20.38941866349232 29.007921307104827 28.87099084896269 21.731669180440157 64-65 20.375049650814887 28.477493885110167 29.100188888301346 22.0472675757736 66-67 20.875298158484657 28.71180316382718 28.65156043126971 21.761338246418447 68-69 21.063792401324726 28.641452739374657 28.675872961554965 21.618881897745652 70-71 20.996705085556627 28.57869784698333 28.429086932212204 21.995510135247837 72-73 20.784073731948137 28.766577244204793 28.802795109112466 21.646553914734596 74-75 21.22819121478012 28.20738680123264 28.52910548738813 22.035316496599112 76-77 20.84785697728255 28.71345160483063 28.8125679811064 21.626123436780414 78-79 20.945517114064508 28.64440598604523 28.88328767359118 21.52678922629909 80-81 20.93310440975067 28.4936387628904 28.64079953149569 21.932457295863237 82-83 20.346969020216985 28.767264145694345 28.778899355283354 22.10686747880532 84-85 20.590200460839057 28.414058557700717 28.528645815321003 22.467095166139227 86-87 20.830496246130163 28.519251052014987 28.49701033770534 22.153242364149506 88-89 20.481516980449996 28.186043809370403 28.997855579949427 22.334583630230178 90-91 20.80853895834422 28.285922000313548 28.677780202822547 22.227758838519684 92-93 20.177665559753486 28.673262651962332 28.838239131978415 22.310832656305763 94-95 20.08231094004239 28.197014484026628 29.08837821351325 22.63229636241773 96-97 20.558527335810314 28.375020652186443 28.771312095841513 22.29513991616173 98-99 20.463719701355686 28.171612744776347 28.824042284430142 22.540625269437832 100 20.40357564550778 28.099509762740677 28.806785713296595 22.69012887845495 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 65.0 1 56.5 2 50.0 3 52.0 4 48.0 5 46.5 6 79.5 7 141.5 8 221.5 9 259.0 10 235.0 11 247.5 12 283.0 13 345.0 14 415.0 15 490.5 16 633.5 17 803.5 18 1031.0 19 1405.5 20 1923.0 21 2764.0 22 4083.0 23 6012.5 24 8826.5 25 12738.5 26 18443.0 27 26242.0 28 36080.5 29 47291.5 30 58994.0 31 71147.0 32 83302.0 33 94880.5 34 105219.0 35 114303.5 36 121803.0 37 128909.0 38 134153.5 39 136479.5 40 137593.0 41 136445.5 42 132784.5 43 128607.0 44 122839.5 45 115742.0 46 110054.0 47 105570.5 48 101383.5 49 100535.5 50 113624.0 51 108818.0 52 83240.5 53 70569.5 54 64552.5 55 59463.0 56 54232.5 57 48939.0 58 43444.0 59 37828.0 60 32143.5 61 26669.0 62 22010.0 63 17655.5 64 13856.0 65 11094.5 66 8611.0 67 6525.0 68 4993.0 69 3710.5 70 2698.0 71 1959.0 72 1468.0 73 1070.0 74 753.0 75 542.0 76 346.5 77 205.5 78 128.0 79 69.0 80 35.0 81 17.0 82 8.0 83 5.5 84 3.0 85 3.5 86 2.5 87 0.5 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content warn #Base N-Count 1 0.9224423050914143 2 6.646195271292095 3 0.0 4 0.004948790773732679 5 0.16647609211513162 6 0.057295316784085185 7 0.03648580526969373 8 0.04244894446288714 9 0.04078910159467867 10-11 0.04300222541895663 12-13 0.041327013635301786 14-15 0.04392436034573912 16-17 0.03468764216246788 18-19 0.029108725855433837 20-21 0.023391489309382417 22-23 0.023529809548399786 24-25 0.019918114418501717 26-27 0.012448821511563572 28-29 0.020502133205463957 30-31 0.02706466010106599 32-33 0.001121930827585359 34-35 0.0029047250193648335 36-37 0.0036116951298980735 38-39 0.005655760884265919 40-41 0.006101459432210788 42-43 0.006885274119975901 44-45 0.008084049524793135 46-47 0.01675211783654851 48-49 0.02577367120357051 50-51 0.022484723298046305 52-53 0.02806363960508035 54-55 0.024928380854019893 56-57 0.016198836880479017 58-59 0.014185508957003922 60-61 0.011634268992905709 62-63 0.01764351493243825 64-65 0.018565649859220733 66-67 0.01984126984126984 68-69 0.02678801962303124 70-71 0.027156873593744236 72-73 0.02402161484268378 74-75 0.024252148574379403 76-77 0.017766466256009247 78-79 0.013970344140754674 80-81 0.012756199820491069 82-83 0.008084049524793135 84-85 0.010466231418981226 86-87 0.008514379157291628 88-89 0.006946749781761402 90-91 0.007069701105332399 92-93 0.003965180185164693 94-95 0.0014293091365128545 96-97 9.989795040143607E-4 98-99 5.686498715158669E-4 100 0.11277710154549814 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 3253320.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 85.72573819784232 #Duplication Level Percentage of deduplicated Percentage of total 1 90.75316091330396 77.7988171308055 2 7.2649881062509785 12.455929368138193 3 1.2488655090152754 3.2117975301047577 4 0.33607569633567924 1.1524134863491997 5 0.13801245983547403 0.5915609999948038 6 0.066264000832893 0.34083182324053135 7 0.046862509930721934 0.28121262803303976 8 0.03028776955677665 0.20771531228966517 9 0.019224099594235818 0.14832001160142358 >10 0.0877966975539637 1.2815688943549264 >50 0.0047709147424530775 0.2837534460086888 >100 0.0025050247618829766 0.40001424157770293 >500 6.470717937010511E-4 0.4194818139559649 >1k 4.67329626511345E-4 0.8399805879485319 >5k 3.5948432808565004E-5 0.24974144934273407 >10k+ 3.5948432808565004E-5 0.33686127625437146 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 10931 0.33599522948864546 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 8104 0.24909938155484243 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 3454 0.10616846790355698 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 3.073783089274956E-5 0.0 4 0.0 0.0 0.0 3.073783089274956E-5 0.0 5 0.0 0.0 0.0 3.073783089274956E-5 0.0 6 0.0 0.0 0.0 3.073783089274956E-5 0.0 7 0.0 0.0 0.0 3.073783089274956E-5 0.0 8 0.0 0.0 0.0 3.073783089274956E-5 0.0 9 0.0 0.0 0.0 3.073783089274956E-5 0.0 10-11 0.0 0.0 0.0 3.073783089274956E-5 0.0 12-13 0.0 0.0 0.0 7.684457723187391E-5 0.0 14-15 0.0 0.0 0.0 1.3832023901737302E-4 0.0 16-17 0.0 0.0 0.0 1.536891544637478E-4 0.0 18-19 0.0 0.0 0.0 1.8442698535649736E-4 0.0 20-21 0.0 0.0 0.0 1.9979590080287213E-4 0.0 22-23 0.0 0.0 0.0 2.612715625883713E-4 0.0 24-25 0.0 0.0 0.0 3.5348505526661996E-4 0.0 26-27 0.0 1.536891544637478E-5 0.0 5.225431251767425E-4 0.0 28-29 0.0 4.610674633912434E-5 0.0 0.001198775404817233 0.0 30-31 0.0 7.684457723187391E-5 0.0 0.003565588383558949 0.0 32-33 0.0 9.221349267824868E-5 0.0 0.010420124672642101 0.0 34-35 0.0 9.221349267824868E-5 0.0 0.019764425264037967 0.0 36-37 0.0 9.221349267824868E-5 0.0 0.03416509903729113 3.073783089274956E-5 38-39 3.073783089274956E-5 9.221349267824868E-5 0.0 0.05893979073684728 3.073783089274956E-5 40-41 3.073783089274956E-5 9.221349267824868E-5 0.0 0.08801777876138836 3.073783089274956E-5 42-43 3.073783089274956E-5 9.221349267824868E-5 0.0 0.11740314509485694 3.073783089274956E-5 44-45 3.073783089274956E-5 9.221349267824868E-5 0.0 0.15256722363616243 4.610674633912434E-5 46-47 3.073783089274956E-5 9.221349267824868E-5 0.0 0.19626105025020596 6.147566178549913E-5 48-49 3.073783089274956E-5 9.221349267824868E-5 0.0 0.24184525346415353 6.147566178549913E-5 50-51 3.073783089274956E-5 9.221349267824868E-5 0.0 0.2909643072307674 9.221349267824868E-5 52-53 3.073783089274956E-5 9.221349267824868E-5 0.0 0.3429573481858532 9.221349267824868E-5 54-55 3.073783089274956E-5 9.221349267824868E-5 0.0 0.4001297136463674 9.221349267824868E-5 56-57 6.147566178549913E-5 1.0758240812462347E-4 0.0 0.47170275288013475 9.221349267824868E-5 58-59 6.147566178549913E-5 1.2295132357099825E-4 0.0 0.5488547084209361 9.221349267824868E-5 60-61 6.147566178549913E-5 1.2295132357099825E-4 0.0 0.6296337280070821 9.221349267824868E-5 62-63 6.147566178549913E-5 1.2295132357099825E-4 0.0 0.7161299841392793 9.221349267824868E-5 64-65 9.221349267824868E-5 1.3832023901737302E-4 0.0 0.8136764904774201 9.221349267824868E-5 66-67 9.221349267824868E-5 1.536891544637478E-4 0.0 0.9254085057725647 9.221349267824868E-5 68-69 9.221349267824868E-5 1.536891544637478E-4 0.0 1.05659756802282 9.221349267824868E-5 70-71 1.0758240812462347E-4 1.536891544637478E-4 0.0 1.2090879470817504 9.221349267824868E-5 72-73 1.2295132357099825E-4 1.536891544637478E-4 0.0 1.3605332398903274 9.221349267824868E-5 74-75 1.2295132357099825E-4 1.536891544637478E-4 0.0 1.5186947487489704 9.221349267824868E-5 76-77 1.2295132357099825E-4 1.536891544637478E-4 0.0 1.6959290816765642 9.221349267824868E-5 78-79 1.3832023901737302E-4 1.536891544637478E-4 0.0 1.8912526280845414 9.221349267824868E-5 80-81 1.536891544637478E-4 1.536891544637478E-4 0.0 2.1095834409157415 9.221349267824868E-5 82-83 1.536891544637478E-4 1.536891544637478E-4 0.0 2.35221250906766 9.221349267824868E-5 84-85 1.536891544637478E-4 1.8442698535649736E-4 0.0 2.596747322734929 9.221349267824868E-5 86-87 1.536891544637478E-4 2.1516481624924693E-4 0.0 2.856758634256698 9.221349267824868E-5 88 1.536891544637478E-4 2.1516481624924693E-4 0.0 3.080822052549396 9.221349267824868E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GAGTACT 4540 0.0 15.508713 12-13 GTACTAG 820 0.0 15.36159 1 CTTACAC 1735 0.0 15.169229 3 TATACTG 2065 0.0 14.794548 5 AGTACTT 5385 0.0 14.774893 12-13 GTATAGG 865 0.0 14.562433 1 CTGTCGC 520 9.276846E-11 14.443208 9 CATGGGG 4515 0.0 14.261698 4 CCTACAC 1190 0.0 14.217737 3 GTATAAA 2250 0.0 13.660209 1 TGCACCG 345 6.986382E-6 13.623519 5 GTATCAA 16780 0.0 13.542368 1 CTACACT 1495 0.0 13.204333 4 CCGTAGC 285 2.9341795E-4 13.176261 9 GTACATG 19675 0.0 12.830187 1 TACATCG 295 3.883894E-4 12.746072 5 CATGGGT 2495 0.0 12.621549 4 GTCCTAG 1120 0.0 12.596504 1 ACATGGG 18885 0.0 12.592399 3 GTACTTT 5650 0.0 12.586197 14-15 >>END_MODULE