FastQCFastQC Report
Wed 25 May 2016
SRR1295085_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295085_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2912571
Sequences flagged as poor quality0
Sequence length100
%GC45

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGTAC1851.9473657E-523.4763031
GTATAGG7450.019.9874941
GTATTAG10450.017.8118211
GTACGAC1900.001224246317.7405853
GTACATG92600.016.8846841
GTATAGA9750.015.908851
TACATGG94000.015.6399642
GTATTAA17150.015.5563511
TCGTACC2450.003451217715.1914942
CATGGGG44200.015.1253334
TACGGGT2250.003786679614.9812824
TACCGTC2356.5886707E-414.8291447
CGTACCC2707.5504556E-414.5648013
ACATGGG92300.014.5467673
TATACTG13250.014.3564115
GTCTAGG8750.014.1816031
GTATATA13250.014.0478131
GCGTGCG3253.2493062E-513.899339
ATACCGT3354.3712047E-513.467856
TACACTG17500.013.1582455
Produced by FastQC (version 0.11.5)