##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1295085_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2912571
Sequences flagged as poor quality	0
Sequence length	100
%GC	45
>>END_MODULE
>>Per base sequence quality	warn
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	26.589821158007823	33.0	27.0	33.0	6.0	33.0
2	22.6482114942434	27.0	6.0	33.0	2.0	33.0
3	25.07447200428762	33.0	15.0	33.0	15.0	33.0
4	25.134309858884126	33.0	6.0	37.0	2.0	37.0
5	26.983072687326764	33.0	15.0	37.0	2.0	37.0
6	27.74062778212102	33.0	15.0	37.0	2.0	37.0
7	27.748172662572003	33.0	15.0	37.0	6.0	37.0
8	28.653525356120074	33.0	27.0	37.0	6.0	37.0
9	29.617670092849238	37.0	27.0	37.0	15.0	37.0
10-11	29.101924210602935	35.0	24.5	37.0	10.5	37.0
12-13	27.925837344394353	33.0	15.0	37.0	4.0	37.0
14-15	29.716372922754502	33.0	27.0	37.0	10.5	40.0
16-17	30.113341786346155	37.0	27.0	40.0	6.0	40.0
18-19	30.258981497790096	37.0	27.0	40.0	10.5	40.0
20-21	29.74609288494598	35.0	27.0	40.0	6.0	40.0
22-23	30.51330920344946	37.0	27.0	40.0	15.0	40.0
24-25	30.628893338565824	37.0	27.0	40.0	15.0	40.0
26-27	29.191652667007943	33.0	24.5	40.0	4.0	40.0
28-29	29.901478968238028	33.0	27.0	40.0	10.5	40.0
30-31	30.616135366313816	37.0	27.0	40.0	15.0	40.0
32-33	29.399230782700233	33.0	27.0	40.0	6.0	40.0
34-35	28.99697123263261	33.0	22.0	37.0	6.0	40.0
36-37	29.33073082166924	33.0	24.5	37.0	6.0	40.0
38-39	29.4275348137436	33.0	24.5	37.0	6.0	40.0
40-41	28.443430735250743	33.0	22.0	37.0	6.0	40.0
42-43	29.084302837596063	33.0	22.0	37.0	6.0	40.0
44-45	28.913143061576868	33.0	22.0	37.0	6.0	40.0
46-47	28.60770827560942	33.0	22.0	37.0	6.0	40.0
48-49	28.545394258200055	33.0	22.0	37.0	6.0	40.0
50-51	28.492231949023733	33.0	22.0	37.0	6.0	40.0
52-53	28.67254257492779	33.0	22.0	37.0	6.0	40.0
54-55	28.61603287954182	33.0	22.0	37.0	6.0	40.0
56-57	28.62154845323942	33.0	22.0	37.0	6.0	40.0
58-59	28.448852577327727	33.0	22.0	37.0	6.0	40.0
60-61	28.3849183419048	33.0	22.0	37.0	10.5	40.0
62-63	28.32376721460181	33.0	22.0	37.0	10.5	40.0
64-65	27.87853617989055	33.0	22.0	37.0	6.0	40.0
66-67	28.07348490388732	33.0	22.0	37.0	10.5	38.5
68-69	27.632778394071767	33.0	22.0	37.0	6.0	37.0
70-71	27.760697164120636	33.0	22.0	37.0	10.5	37.0
72-73	27.812031706694874	33.0	22.0	37.0	10.5	37.0
74-75	27.90845253214428	33.0	22.0	37.0	15.0	37.0
76-77	27.677113450624894	33.0	22.0	37.0	15.0	37.0
78-79	27.1966061943211	30.0	22.0	33.0	10.5	37.0
80-81	27.011908894238115	27.0	22.0	33.0	10.5	37.0
82-83	27.1383813476135	27.0	22.0	33.0	15.0	37.0
84-85	27.104964823175123	27.0	22.0	33.0	15.0	37.0
86-87	26.909475339828624	27.0	22.0	33.0	15.0	37.0
88-89	26.862620688045027	27.0	22.0	33.0	15.0	37.0
90-91	27.022484602092103	27.0	22.0	33.0	15.0	37.0
92-93	26.952733341092802	27.0	22.0	33.0	15.0	37.0
94-95	26.812224148355526	27.0	22.0	33.0	15.0	37.0
96-97	26.918714256236157	27.0	22.0	33.0	15.0	37.0
98-99	26.999527221825666	27.0	22.0	33.0	15.0	37.0
100	26.682523790836342	27.0	22.0	33.0	15.0	37.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	7.262739960458749
1101	2	11.332318048611501
1101	3	8.548195856634184
1101	4	12.98082419506909
1101	5	10.82467189515339
1101	6	9.916029342947681
1101	7	9.497889987378585
1101	8	9.030892658853663
1101	9	7.372518538016319
1101	10-11	7.754802743364845
1101	12-13	9.240689270628625
1101	14-15	8.06673433985284
1101	16-17	8.203630255210317
1101	18-19	8.211121562363562
1101	20-21	7.329492095520667
1101	22-23	7.507781993810347
1101	24-25	7.449352154177674
1101	26-27	8.810833660337074
1101	28-29	8.473058322630013
1101	30-31	5.6158429528216836
1101	32-33	7.278144407653098
1101	34-35	8.874648036284295
1101	36-37	8.308508671429415
1101	38-39	8.413914418364676
1101	40-41	9.200581241063087
1101	42-43	8.712095804436618
1101	44-45	8.626370861258312
1101	46-47	7.498799607803463
1101	48-49	5.56931496407622
1101	50-51	6.871723626405085
1101	52-53	6.090356365347176
1101	54-55	4.066998225086646
1101	56-57	6.317917823469884
1101	58-59	6.367941752303597
1101	60-61	6.801331888915634
1101	62-63	6.306861171655402
1101	64-65	6.715370257464432
1101	66-67	5.836308362761844
1101	68-69	6.8766683628001
1101	70-71	6.378041543822391
1101	72-73	5.6019586666634
1101	74-75	4.85090321886841
1101	76-77	5.101849891533561
1101	78-79	5.191521155341295
1101	80-81	4.858178695641705
1101	82-83	4.456807538056356
1101	84-85	4.317988694120267
1101	86-87	4.088767240523907
1101	88-89	4.028264023715256
1101	90-91	3.8662538127509123
1101	92-93	3.7674000846277877
1101	94-95	3.581092689251559
1101	96-97	3.7889155686946125
1101	98-99	3.598795906981664
1101	100	3.73206419645847
1102	1	7.071975190350489
1102	2	10.643625366251854
1102	3	8.316901687269109
1102	4	12.788335406143908
1102	5	10.694326177541075
1102	6	9.813987258910217
1102	7	6.958365672625916
1102	8	8.06931239106208
1102	9	6.641390613500636
1102	10-11	7.621785261972324
1102	12-13	9.289689243153429
1102	14-15	6.334354178497787
1102	16-17	7.576345433783171
1102	18-19	7.716394435862977
1102	20-21	7.197006746967933
1102	22-23	6.863374550182279
1102	24-25	7.2099152971570035
1102	26-27	8.799627961105266
1102	28-29	7.905843837706332
1102	30-31	4.298537705178578
1102	32-33	4.582334380122159
1102	34-35	5.275971116325518
1102	36-37	5.503125344652965
1102	38-39	4.791658901823013
1102	40-41	6.528058757193811
1102	42-43	7.292245283443183
1102	44-45	6.594511728184383
1102	46-47	5.542032078627983
1102	48-49	3.736446859156011
1102	50-51	5.319862923462132
1102	52-53	6.278511439737265
1102	54-55	5.673136651896705
1102	56-57	6.492488802255849
1102	58-59	6.150412357825079
1102	60-61	6.219446793771816
1102	62-63	4.376147395910348
1102	64-65	6.251352063707749
1102	66-67	3.917970495689236
1102	68-69	6.308905568516845
1102	70-71	5.499974201916263
1102	72-73	5.284294474489933
1102	74-75	4.519380104463288
1102	76-77	3.577273726306487
1102	78-79	4.626960042739178
1102	80-81	4.568728991975636
1102	82-83	4.021278609660701
1102	84-85	3.149642489918236
1102	86-87	3.355627753330534
1102	88-89	3.4236411377126323
1102	90-91	3.4363026479220338
1102	92-93	3.43126305347808
1102	94-95	3.278710529971871
1102	96-97	3.4700992598957043
1102	98-99	3.343035522950089
1102	100	3.454889833285744
1103	1	6.772537357243898
1103	2	10.984153761277462
1103	3	7.9951103601090665
1103	4	12.034848453682152
1103	5	10.459424368301942
1103	6	9.135985012599477
1103	7	8.230142300022784
1103	8	8.273999888522379
1103	9	7.206433052844556
1103	10-11	7.607811750397001
1103	12-13	8.933909310493984
1103	14-15	8.748170823180839
1103	16-17	8.183382668577902
1103	18-19	7.429669993133334
1103	20-21	8.588121578226438
1103	22-23	7.053692292623737
1103	24-25	5.497585370470929
1103	26-27	6.13701038331741
1103	28-29	7.283732183709699
1103	30-31	6.28909346289397
1103	32-33	8.199411822375556
1103	34-35	7.40349714923023
1103	36-37	4.11998236664958
1103	38-39	6.553977902734864
1103	40-41	6.897350687785739
1103	42-43	7.651198719225835
1103	44-45	5.642782469174353
1103	46-47	6.456982672388701
1103	48-49	5.795836467490119
1103	50-51	6.573658525236105
1103	52-53	6.2909066315432725
1103	54-55	5.163712397023328
1103	56-57	6.02368932896858
1103	58-59	5.541099250228093
1103	60-61	5.254902023189711
1103	62-63	2.7827014483719417
1103	64-65	5.105149204537778
1103	66-67	4.287793095858927
1103	68-69	5.369450916524343
1103	70-71	3.9815407547638912
1103	72-73	4.584244361199151
1103	74-75	3.3986183753322763
1103	76-77	3.73653768569735
1103	78-79	2.9908095307770033
1103	80-81	4.256352522812023
1103	82-83	3.620520271532424
1103	84-85	3.415894617168
1103	86-87	2.85171712870336
1103	88-89	3.2200291255172147
1103	90-91	2.877006398830229
1103	92-93	2.5121715862080656
1103	94-95	2.9149525471979203
1103	96-97	3.0618978079207544
1103	98-99	2.914085411122173
1103	100	3.0795704410574594
1104	1	6.648273831195642
1104	2	9.987671160766574
1104	3	7.661385044573574
1104	4	12.515437269520042
1104	5	9.877613678844451
1104	6	8.32346426896816
1104	7	4.128732813667199
1104	8	6.830691247614308
1104	9	6.866679177367839
1104	10-11	7.428576257157683
1104	12-13	7.812222640676428
1104	14-15	7.595830038479345
1104	16-17	7.54748221984207
1104	18-19	7.261747224224099
1104	20-21	7.842941643831384
1104	22-23	6.7850243294768475
1104	24-25	6.3748182047669175
1104	26-27	8.598146123167759
1104	28-29	7.543048012158788
1104	30-31	6.633589127598999
1104	32-33	7.938369058886938
1104	34-35	8.349264383527224
1104	36-37	6.910521438489603
1104	38-39	7.710428680074877
1104	40-41	7.5581441988281135
1104	42-43	7.675619852500727
1104	44-45	7.058676525295926
1104	46-47	6.945941996966141
1104	48-49	5.863562185604749
1104	50-51	5.928472498908306
1104	52-53	6.279864729381906
1104	54-55	5.017573925460546
1104	56-57	6.231488339246141
1104	58-59	4.148419947212016
1104	60-61	4.448278943858131
1104	62-63	4.039294647478123
1104	64-65	2.9413217074013005
1104	66-67	3.5893977955109797
1104	68-69	4.46209992170181
1104	70-71	2.705383838269519
1104	72-73	2.6992309751296233
1104	74-75	3.5536345729827623
1104	76-77	4.106443130757668
1104	78-79	4.619898394797026
1104	80-81	3.2538402922260516
1104	82-83	3.173473783560361
1104	84-85	3.195868257421992
1104	86-87	0.9298736764452684
1104	88-89	0.4288992614847693
1104	90-91	2.426459424704184
1104	92-93	2.7042814422870656
1104	94-95	2.7429839936816727
1104	96-97	3.1322214456325206
1104	98-99	2.810484316724988
1104	100	0.9564255442021832
1105	1	5.918872807379245
1105	2	-2.6204290293552255
1105	3	0.5601716081568071
1105	4	8.6278605431379
1105	5	8.669005887457207
1105	6	8.289319388985746
1105	7	5.440179333535067
1105	8	6.30636768951938
1105	9	5.1627028019379715
1105	10-11	5.094346691592321
1105	12-13	5.202024937839507
1105	14-15	5.973357943899945
1105	16-17	6.292596778496996
1105	18-19	6.375651554708828
1105	20-21	7.226021389090963
1105	22-23	2.1163839831158455
1105	24-25	4.204141343144997
1105	26-27	7.118450252188403
1105	28-29	6.162947629270395
1105	30-31	5.590084352914818
1105	32-33	7.296349527784354
1105	34-35	6.437967174137231
1105	36-37	6.376688575904687
1105	38-39	4.168485403630342
1105	40-41	5.452087867705284
1105	42-43	5.760812597661097
1105	44-45	6.222937442611922
1105	46-47	5.509217551894018
1105	48-49	5.162814546028514
1105	50-51	6.059207108968359
1105	52-53	5.765901696034433
1105	54-55	5.437227106584931
1105	56-57	5.239699945112058
1105	58-59	2.208773243212331
1105	60-61	4.486096476899462
1105	62-63	-0.8438164066718308
1105	64-65	-0.27026207503254085
1105	66-67	0.9403837067193841
1105	68-69	2.921485783443213
1105	70-71	3.110600589351712
1105	72-73	2.8076141213082693
1105	74-75	2.7074727397850324
1105	76-77	1.7599426231773094
1105	78-79	3.294459179152156
1105	80-81	1.7169063859731288
1105	82-83	1.1485491365850855
1105	84-85	1.3478595133036464
1105	86-87	-0.30777867737174347
1105	88-89	1.5922318623608085
1105	90-91	1.5323600476727037
1105	92-93	1.716946489533175
1105	94-95	1.055733073153256
1105	96-97	2.086354652991563
1105	98-99	2.042904296375781
1105	100	2.126169960164887
1106	1	6.081004393168168
1106	2	10.350433456878392
1106	3	6.912695969509013
1106	4	11.941335215138558
1106	5	10.015007856628085
1106	6	9.495392338252437
1106	7	6.6764407537281905
1106	8	7.451085705714885
1106	9	6.9585437937896835
1106	10-11	5.783442338370463
1106	12-13	8.028668509572885
1106	14-15	7.953194293521005
1106	16-17	7.8609539028203095
1106	18-19	7.279585967725868
1106	20-21	8.292698351332856
1106	22-23	6.3783572696984265
1106	24-25	4.994716584093041
1106	26-27	7.772885712322299
1106	28-29	6.341828145266941
1106	30-31	5.413511586947269
1106	32-33	7.4236623498053085
1106	34-35	6.753509412397815
1106	36-37	6.087397654277638
1106	38-39	7.147447538904849
1106	40-41	6.684316757417822
1106	42-43	6.244788829662472
1106	44-45	4.645980544311236
1106	46-47	6.01978258064204
1106	48-49	5.861888159012409
1106	50-51	6.721464780814138
1106	52-53	5.752965299445343
1106	54-55	5.400802801548895
1106	56-57	4.868213597550113
1106	58-59	4.978843803905569
1106	60-61	4.987321069251529
1106	62-63	4.970716018831787
1106	64-65	5.143126758399202
1106	66-67	2.987701235649954
1106	68-69	3.3239572899650476
1106	70-71	2.959506681325685
1106	72-73	2.798917808943294
1106	74-75	2.767211381239033
1106	76-77	3.4587205068163414
1106	78-79	4.129491876130512
1106	80-81	3.7174919073372656
1106	82-83	2.108826773509289
1106	84-85	2.827466618993853
1106	86-87	3.2661191970909584
1106	88-89	3.53510464619087
1106	90-91	3.3358024964177915
1106	92-93	2.928657030509438
1106	94-95	1.6281774650460505
1106	96-97	1.5054928009148512
1106	98-99	1.9313106196527983
1106	100	2.858607237373061
1107	1	6.098395336008103
1107	2	10.431507444060298
1107	3	6.2008645522977694
1107	4	11.42517993883645
1107	5	9.384717696716539
1107	6	7.87309311159272
1107	7	3.5865284099468226
1107	8	6.306278037031724
1107	9	5.635260962782969
1107	10-11	4.498192620770002
1107	12-13	6.153305922044009
1107	14-15	6.113915850568713
1107	16-17	6.9810632221550115
1107	18-19	6.175784997230249
1107	20-21	5.4258422822146315
1107	22-23	5.675391625063419
1107	24-25	4.570705094851185
1107	26-27	7.052238410623708
1107	28-29	6.253194464664563
1107	30-31	4.930721367110085
1107	32-33	4.8957436344310885
1107	34-35	6.722897002398621
1107	36-37	6.4080051803377955
1107	38-39	6.465898566180009
1107	40-41	7.538911705087759
1107	42-43	6.014456983982964
1107	44-45	3.97361176646454
1107	46-47	3.4119589522648575
1107	48-49	4.029581501574292
1107	50-51	4.832723870783489
1107	52-53	3.936081140186978
1107	54-55	5.344831811547486
1107	56-57	5.288397449389983
1107	58-59	2.940047200490408
1107	60-61	2.7745945503614706
1107	62-63	0.3347929618455048
1107	64-65	2.0830450198367565
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2206	74-75	1.6177190602022478
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2206	78-79	0.9871025251999086
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2206	84-85	3.6357612598768867
2206	86-87	-1.605998427957939
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2206	100	1.6576241940205065
2207	1	6.472172457463582
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2207	3	7.876490098619865
2207	4	12.204365177444252
2207	5	10.575362736051726
2207	6	9.448980009574868
2207	7	5.684370231991707
2207	8	7.034573748889915
2207	9	6.1356060135964405
2207	10-11	7.0425539245905036
2207	12-13	8.952762820692381
2207	14-15	8.463453277795995
2207	16-17	7.6497887555540025
2207	18-19	4.321525822029123
2207	20-21	7.681548363135715
2207	22-23	6.5323387149977705
2207	24-25	4.535668591103892
2207	26-27	4.0107134164631795
2207	28-29	5.267420110037012
2207	30-31	5.635857988770642
2207	32-33	6.841336726511795
2207	34-35	6.534757419260938
2207	36-37	6.999151672117179
2207	38-39	7.09116826568178
2207	40-41	4.144866184467386
2207	42-43	5.769628180959117
2207	44-45	4.118192722870344
2207	46-47	5.613234998133038
2207	48-49	2.524627478917928
2207	50-51	5.517102861593639
2207	52-53	2.878752086326543
2207	54-55	3.1430717012275444
2207	56-57	5.332682448719744
2207	58-59	3.822873201769873
2207	60-61	3.658156255359984
2207	62-63	4.46595205364849
2207	64-65	3.682870256967636
2207	66-67	3.7041322318166436
2207	68-69	3.6012719862521614
2207	70-71	3.721263930604664
2207	72-73	3.94717898261208
2207	74-75	3.6374691752463733
2207	76-77	4.172098167014639
2207	78-79	4.371911979726217
2207	80-81	4.351953973731742
2207	82-83	4.016912683385318
2207	84-85	3.483326703405538
2207	86-87	3.554346139959609
2207	88-89	3.243876357346597
2207	90-91	1.403947415621822
2207	92-93	-0.6875792308823208
2207	94-95	1.4651183074960379
2207	96-97	-0.842280587594999
2207	98-99	0.7923696409583414
2207	100	2.215571351177619
2208	1	1.1631755654222822
2208	2	8.541784634958741
2208	3	6.627398194272541
2208	4	11.770276525724299
2208	5	10.146514697042246
2208	6	9.33253312130018
2208	7	7.697065922712532
2208	8	7.76718904309482
2208	9	6.531485722375319
2208	10-11	6.847542933736836
2208	12-13	7.115519081746214
2208	14-15	7.92416468692198
2208	16-17	7.2661602270673775
2208	18-19	6.539362440917412
2208	20-21	6.681334986525567
2208	22-23	5.934085446486826
2208	24-25	6.326016510055787
2208	26-27	8.215185747662368
2208	28-29	7.234976215804114
2208	30-31	6.152601343851661
2208	32-33	8.430834289144581
2208	34-35	6.641123568205256
2208	36-37	6.893573754647754
2208	38-39	7.260188191566165
2208	40-41	7.741954633303077
2208	42-43	7.0935042959401535
2208	44-45	5.9488017571576925
2208	46-47	4.649991585174881
2208	48-49	4.718116511418188
2208	50-51	6.060160743274977
2208	52-53	5.735901643651069
2208	54-55	6.516304744169485
2208	56-57	6.4566430295064094
2208	58-59	5.704827270242443
2208	60-61	5.240178903645607
2208	62-63	3.5470198379772917
2208	64-65	3.4638702931348
2208	66-67	2.9110533291787526
2208	68-69	5.566879332093723
2208	70-71	5.446844614282082
2208	72-73	4.7355878724435065
2208	74-75	2.149468539226447
2208	76-77	3.3446076200384702
2208	78-79	4.2690495008305795
2208	80-81	3.7129240506237196
2208	82-83	1.4852753822334144
2208	84-85	2.0894249711433304
2208	86-87	1.1522927128353366
2208	88-89	0.7717144258226476
2208	90-91	1.8980953698898944
2208	92-93	0.8734879775762323
2208	94-95	-1.0737335696814263
2208	96-97	1.424782880753071
2208	98-99	0.16652130816316202
2208	100	1.7925723600868295
2209	1	5.475731850937414
2209	2	9.684088285804581
2209	3	5.721871602677123
2209	4	-3.412699885816039
2209	5	0.6172084185199793
2209	6	4.456176090151462
2209	7	2.260492942599111
2209	8	5.499229767464481
2209	9	5.190298713366218
2209	10-11	5.3477367660461645
2209	12-13	7.446155454254896
2209	14-15	6.9428031018618945
2209	16-17	6.454027191290304
2209	18-19	5.503600438412729
2209	20-21	6.137838257287658
2209	22-23	5.6154587823671065
2209	24-25	5.439075189987946
2209	26-27	7.110691778133621
2209	28-29	6.424870219938743
2209	30-31	4.343288024676472
2209	32-33	6.343518631893513
2209	34-35	5.64416659958702
2209	36-37	6.382972036282389
2209	38-39	6.980623364544755
2209	40-41	7.324196658406077
2209	42-43	6.934072805588027
2209	44-45	6.588589675937033
2209	46-47	6.912245012977692
2209	48-49	5.393164691889417
2209	50-51	4.8110058371505815
2209	52-53	5.036203775136467
2209	54-55	5.6138839557316125
2209	56-57	5.298371194709077
2209	58-59	3.2930457798364365
2209	60-61	4.251911216775355
2209	62-63	4.352266485491249
2209	64-65	5.162794050599185
2209	66-67	4.645993795672258
2209	68-69	3.959481755039757
2209	70-71	3.8010316358010314
2209	72-73	1.1352662757338123
2209	74-75	2.4698764505832855
2209	76-77	3.429925227888205
2209	78-79	3.6228805305955305
2209	80-81	3.721558115698617
2209	82-83	3.0750771836603867
2209	84-85	3.0658948087603974
2209	86-87	1.6270752910355704
2209	88-89	2.0727872012103568
2209	90-91	2.3412184132061427
2209	92-93	2.3614169381519936
2209	94-95	0.8888059821597203
2209	96-97	0.3062056961137216
2209	98-99	0.25161916208762847
2209	100	0.1721778042774389
2210	1	1.7415411161990662
2210	2	-17.217415242885203
2210	3	-7.7862761506154605
2210	4	-6.935864906385216
2210	5	-0.6444404860940551
2210	6	2.852729754449925
2210	7	-6.8917610133864535
2210	8	-7.0522182872614145
2210	9	-2.696645674010437
2210	10-11	-0.8311381091654155
2210	12-13	3.6287670254036577
2210	14-15	-4.140590738033868
2210	16-17	-0.7945013590179393
2210	18-19	-0.23185698067934268
2210	20-21	2.5178884936840475
2210	22-23	2.693513961637226
2210	24-25	2.1654512120415177
2210	26-27	4.108747304900771
2210	28-29	3.4108269284604766
2210	30-31	2.582156989045064
2210	32-33	4.6432026301780205
2210	34-35	2.544572865691425
2210	36-37	4.108756974037327
2210	38-39	3.2629938494503854
2210	40-41	4.183464417692985
2210	42-43	2.696366259497399
2210	44-45	3.394900347509651
2210	46-47	2.6893989702460033
2210	48-49	2.9183332330378526
2210	50-51	2.0777730098665828
2210	52-53	2.417901245853564
2210	54-55	2.864876629856301
2210	56-57	2.963996659834187
2210	58-59	2.0694498697407084
2210	60-61	2.799526521820397
2210	62-63	1.9437635725906297
2210	64-65	2.6204156173777626
2210	66-67	2.6445835651833143
2210	68-69	3.382757906787095
2210	70-71	2.423224095672083
2210	72-73	1.1109799324823548
2210	74-75	0.3779739220062339
2210	76-77	-0.3827390229954162
2210	78-79	0.3187022487389264
2210	80-81	0.9391901759372274
2210	82-83	0.9021505480879171
2210	84-85	-0.8383532507707301
2210	86-87	-0.6887080001807142
2210	88-89	-1.469816596049828
2210	90-91	-1.6419134736572403
2210	92-93	-1.1041581534782878
2210	94-95	-0.41351768702338276
2210	96-97	-0.04068999824201569
2210	98-99	-0.3127986931783653
2210	100	-0.09764545887031773
2211	1	0.9638723282304973
2211	2	-17.217415242885203
2211	3	-8.044884889987527
2211	4	-19.24132829943985
2211	5	-21.336765193017303
2211	6	-13.233782797470552
2211	7	-11.22474011623654
2211	8	-19.542837219329964
2211	9	-15.851822621931209
2211	10-11	-9.653371377284426
2211	12-13	-22.629251112176522
2211	14-15	-18.177095413631385
2211	16-17	-16.24163319397265
2211	18-19	-19.336168512513968
2211	20-21	-8.301802068848652
2211	22-23	-3.8958360254260143
2211	24-25	-1.8620819236083506
2211	26-27	-17.65576426921298
2211	28-29	-3.9554524516525014
2211	30-31	-4.515748602817755
2211	32-33	-0.8003100955929021
2211	34-35	-5.329301819969832
2211	36-37	-6.181186145035532
2211	38-39	-2.4819544075968807
2211	40-41	-5.0542605574333415
2211	42-43	-1.5419942083806717
2211	44-45	0.08317809359169459
2211	46-47	0.8670567803343943
2211	48-49	0.49454135079255224
2211	50-51	-4.335893920432447
2211	52-53	-1.0223805142964864
2211	54-55	-0.5845668854813084
2211	56-57	-6.313171634741927
2211	58-59	-2.1306488087510544
2211	60-61	-1.138299825255288
2211	62-63	-0.35291621039830545
2211	64-65	-3.408864960099546
2211	66-67	-2.474855648784139
2211	68-69	0.18617672066999447
2211	70-71	-0.3873671101072631
2211	72-73	-1.228224607870942
2211	74-75	-1.8963933399145603
2211	76-77	-1.7907685873653811
2211	78-79	-1.1822178118883535
2211	80-81	-0.9758359301964283
2211	82-83	-1.664323046641396
2211	84-85	-1.7787977202452865
2211	86-87	-2.111271257664651
2211	88-89	-2.9231822345502785
2211	90-91	-2.504373979815057
2211	92-93	-2.4341220121746048
2211	94-95	-2.5654891347405133
2211	96-97	-3.2603105308773337
2211	98-99	-3.0960935019061466
2211	100	-2.5554820737328185
2212	1	-21.457529885791644
2212	2	-17.217415242885203
2212	3	-20.236397805116678
2212	4	-19.24132829943985
2212	5	-21.336765193017303
2212	6	-22.051494974592323
2212	7	-22.67123458118119
2212	8	-23.21479293888716
2212	9	-24.569903802743017
2212	10-11	-11.730896881951237
2212	12-13	-22.629251112176522
2212	14-15	-24.091486557542826
2212	16-17	-24.47631954083612
2212	18-19	-19.13651146888471
2212	20-21	-24.189255943387398
2212	22-23	-19.225153368599447
2212	24-25	-25.751934322132335
2212	26-27	-17.73455089825574
2212	28-29	-18.636276198125262
2212	30-31	-6.493553030862035
2212	32-33	-17.78511691130812
2212	34-35	-14.18009300729347
2212	36-37	-18.21050782942885
2212	38-39	-24.23324592051201
2212	40-41	-10.703965354481314
2212	42-43	-14.327575931844965
2212	44-45	-17.658940421711108
2212	46-47	-17.517870504232572
2212	48-49	-18.150761449294272
2212	50-51	-9.500790816460231
2212	52-53	-2.329436081910984
2212	54-55	-23.98438238363629
2212	56-57	-23.910588608911667
2212	58-59	-12.826814426289584
2212	60-61	-6.455002668754311
2212	62-63	-3.2585304930117225
2212	64-65	-17.708787903446627
2212	66-67	-18.01164748729076
2212	68-69	-14.936354210521673
2212	70-71	-9.96874761802999
2212	72-73	-17.884061378003352
2212	74-75	-6.611895836115991
2212	76-77	-11.331697608420297
2212	78-79	-5.020003789748134
2212	80-81	-2.58434580647166
2212	82-83	-3.0723401944599296
2212	84-85	-5.449468438717176
2212	86-87	-5.150986907762327
2212	88-89	-3.622849045924273
2212	90-91	-4.45500128608812
2212	92-93	-5.636353399194359
2212	94-95	-4.024544919416005
2212	96-97	-5.657591841925736
2212	98-99	-4.013910953610079
2212	100	-4.1934634064873215
2213	1	-21.457529885791644
2213	2	-17.217415242885203
2213	3	-20.236397805116678
2213	4	-19.24132829943985
2213	5	-21.336765193017303
2213	6	-22.051494974592323
2213	7	-10.954374348902455
2213	8	-10.805902270084598
2213	9	-4.36926303382031
2213	10-11	-18.05067308398191
2213	12-13	-16.65007610216451
2213	14-15	-24.091486557542826
2213	16-17	-18.40883856366351
2213	18-19	-19.04346546073375
2213	20-21	-18.152211490043385
2213	22-23	-20.42807086960065
2213	24-25	-7.925342411839985
2213	26-27	-23.765727368843976
2213	28-29	-24.65951149224291
2213	30-31	-19.968825357654186
2213	32-33	-23.887763970131648
2213	34-35	-18.405922473240842
2213	36-37	-24.296684300017084
2213	38-39	-11.951307594520824
2213	40-41	-17.619868438181754
2213	42-43	-23.820222990668494
2213	44-45	-17.678592710339814
2213	46-47	-17.444069707982955
2213	48-49	-6.549478262411185
2213	50-51	-23.907849639989642
2213	52-53	-18.270365550214123
2213	54-55	-18.12515130634354
2213	56-57	-18.00830586962452
2213	58-59	-18.417204188121428
2213	60-61	-19.750075579776947
2213	62-63	-17.97804673603276
2213	64-65	-18.03674816163881
2213	66-67	-8.903961793870419
2213	68-69	-23.14223656346285
2213	70-71	-17.780418328765226
2213	72-73	-9.745890160777975
2213	74-75	-4.530116760048241
2213	76-77	-3.387110692356316
2213	78-79	-4.623516004405719
2213	80-81	-3.4578390571960576
2213	82-83	-3.3747549745489778
2213	84-85	-3.1593588955006098
2213	86-87	-3.2551215163514584
2213	88-89	-2.784865701204726
2213	90-91	-2.8517613982226813
2213	92-93	-3.8391730180310795
2213	94-95	-3.775562846811237
2213	96-97	-3.5352844848619682
2213	98-99	-3.929917337741621
2213	100	-3.4759680591293147
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	768.0
3	760.0
4	4268.0
5	14122.0
6	20529.0
7	25600.0
8	24378.0
9	24223.0
10	28094.0
11	33493.0
12	40854.0
13	52909.0
14	38563.0
15	43108.0
16	49988.0
17	53723.0
18	54745.0
19	55882.0
20	56136.0
21	54928.0
22	58400.0
23	63841.0
24	70738.0
25	78896.0
26	87111.0
27	95565.0
28	102814.0
29	108243.0
30	115411.0
31	126885.0
32	146646.0
33	177523.0
34	213440.0
35	248864.0
36	264022.0
37	215864.0
38	61193.0
39	44.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.83504185590532	19.793623784040054	13.563713739931421	24.807620620123213
2	15.437360788317944	21.018602923798912	39.33117255815524	24.212863729727903
3	18.09751944652234	25.587498543846166	30.154876155490122	26.160105854141374
4	12.922054915052117	17.522397986877003	38.107794870958415	31.447752227112463
5	13.440774575055595	37.41892275749724	35.482092777632175	13.658209889814987
6	31.07877186383658	37.09122429847657	18.268534977365285	13.561468860321563
7	26.708854713204378	31.52434482645383	23.708721864891775	18.05807859545002
8	24.540092673055355	36.203531919883815	21.17257575165305	18.08379965540778
9	24.90904372958166	17.205636628749673	21.297754982463797	36.587564659204865
10-11	23.48381097739774	26.860318480194483	29.162039238135	20.493831304272774
12-13	24.697410390131935	24.436586374643436	28.468737253651643	22.397265981572986
14-15	21.91715996089511	26.171004131242544	25.90172206217084	26.010113845691507
16-17	21.916876105579632	29.26672465938619	26.68459134936952	22.131807885664664
18-19	22.007928008337828	28.628372814122617	27.8499507238068	21.513748453732756
20-21	22.84047759852325	27.97789066074302	27.40126266965242	21.780369071081303
22-23	22.580530488176148	27.77823758102817	27.350723861977837	22.29050806881784
24-25	22.534861990758415	27.97094163910403	27.08739420404937	22.406802166088184
26-27	22.38916446360491	27.758444646345424	27.283727342149394	22.568663547900268
28-29	22.41955188845121	27.6339495006384	27.510933950437167	22.435564660473226
30-31	22.676344265502195	27.959394288565463	27.194591169908982	22.169670276023353
32-33	22.21190183267245	27.851101349157904	27.43713160953712	22.49986520863253
34-35	22.12857411111686	27.663229380593375	27.327582835109443	22.880613673180328
36-37	22.60879457016048	27.86851763416246	27.111063589797002	22.41162420588006
38-39	22.539734819106418	27.69334534515611	27.175069874590267	22.5918499611472
40-41	22.41243570783349	27.7037930777292	27.137057677051608	22.746713537385702
42-43	22.48514083726185	27.72646305496258	27.235365496879773	22.553030610895792
44-45	22.84032292192076	27.570216771071753	27.237785231030898	22.35167507597659
46-47	22.44277235225555	27.661824840663	27.488050867882407	22.407351939199042
48-49	22.52727119835827	27.610493753164565	27.3694050879996	22.49282996047756
50-51	22.529902198486738	27.56896667534664	27.387515377813436	22.513615748353182
52-53	22.30134661787342	27.50433005513108	27.188062648219386	23.006260678776112
54-55	22.55241982498843	27.759314553711807	27.232002218966517	22.456263402333246
56-57	22.67183888434853	27.447394363312505	27.05388404447116	22.826882707867806
58-59	22.532079564247155	27.18025513667628	27.39867792878128	22.888987370295286
60-61	22.66020030614445	27.53968038835502	27.34535567525998	22.454763630240553
62-63	22.39447267556787	27.53283671468244	27.302490141180847	22.77020046856885
64-65	22.271455387617113	27.436983178531804	27.477759113061317	22.813802320789765
66-67	22.460218386452784	27.397270178157367	27.180368531768067	22.962142903621782
68-69	22.961918751837086	27.319909522106084	27.054263903038894	22.663907823017933
70-71	22.73159627929848	27.35896261431859	27.113098172401767	22.796342933981165
72-73	22.81464752086693	27.430217621845447	27.167206565749797	22.58792829153783
74-75	22.75729126319331	27.36577442384221	27.027233401975387	22.849700910989096
76-77	22.784290997079903	27.415920900066293	27.089445806662432	22.71034229619137
78-79	22.87700571979793	27.309162711611272	27.1812030655053	22.632628503085495
80-81	22.722142393988083	27.2733464233694	27.219089358980252	22.785421823662258
82-83	22.612529529221813	27.386310765114946	27.22763879772795	22.773520907935293
84-85	22.613596152993452	27.463825636219312	26.953928601547737	22.968649609239492
86-87	22.741884289872026	27.345052352496058	27.089268113294697	22.823795244337223
88-89	22.720071773278278	27.11744649144513	27.267227591240673	22.89525414403592
90-91	22.80647833642345	27.141224278514635	27.210472280320868	22.841825104741044
92-93	22.59689984565884	27.24253264183808	27.277393317118985	22.883174195384097
94-95	22.54755781886648	27.106432921412203	27.253256123518664	23.092753136202653
96-97	22.667842954824803	27.132091358150046	27.232780381198868	22.967285305826287
98-99	22.681847763672206	27.105050769533555	27.213649809149608	22.99945165764463
100	22.661649868345247	26.954970522919513	27.237725698173588	23.145653910561652
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	432.0
1	503.5
2	496.0
3	385.5
4	389.5
5	500.0
6	688.0
7	1012.0
8	1488.0
9	2090.0
10	2832.0
11	3716.0
12	4686.0
13	5662.5
14	6615.5
15	7491.5
16	8305.0
17	9123.0
18	9950.5
19	10762.5
20	11748.5
21	12840.0
22	13931.5
23	15391.0
24	17378.0
25	19940.5
26	23656.0
27	28476.5
28	34516.0
29	41439.0
30	48585.0
31	55626.5
32	62684.0
33	69632.5
34	75897.5
35	81514.5
36	86476.5
37	91057.0
38	94356.5
39	96697.0
40	97902.5
41	97999.0
42	97634.5
43	96513.5
44	94443.0
45	92434.5
46	91425.5
47	90758.0
48	90455.5
49	90048.5
50	89237.0
51	85554.0
52	78912.0
53	73334.0
54	69307.5
55	65374.5
56	61128.0
57	56916.0
58	52845.5
59	48826.0
60	44038.5
61	37815.0
62	31283.0
63	25403.5
64	20477.0
65	16448.0
66	13023.0
67	10241.0
68	8214.0
69	6737.0
70	5317.0
71	3868.5
72	2782.5
73	2101.5
74	1572.5
75	1118.0
76	752.5
77	479.5
78	338.0
79	243.5
80	159.0
81	108.5
82	64.5
83	49.5
84	35.5
85	22.0
86	19.5
87	14.5
88	12.5
89	10.0
90	3.5
91	0.5
92	0.5
93	0.5
94	0.5
95	1.0
96	1.0
97	2.0
98	2.0
99	0.5
100	2.0
>>END_MODULE
>>Per base N content	fail
#Base	N-Count
1	8.172779307354224
2	24.746692870319727
3	6.275452169234673
4	24.22097178060209
5	12.48954961097944
6	10.08751374644601
7	7.993727878221681
8	8.653351283110352
9	5.130518706668438
10-11	6.914234880454416
12-13	9.973885615148953
14-15	7.459251637127473
16-17	8.115613318954285
18-19	6.755423301268879
20-21	8.182341306014514
22-23	5.831686163187095
24-25	5.438133525328652
26-27	9.966229149435327
28-29	6.514673805376761
30-31	4.352031246620254
32-33	8.93794520373924
34-35	7.390017273398657
36-37	7.038935703198308
38-39	6.284413324173041
40-41	8.854479427282632
42-43	6.642155676204975
44-45	6.778392698409756
46-47	6.1207778282486505
48-49	4.049892689311265
50-51	6.715321274571504
52-53	4.700778109786851
54-55	5.181847927484
56-57	4.866542309183193
58-59	3.854601312723364
60-61	4.370176727022277
62-63	3.2196640013239164
64-65	4.641861091111599
66-67	2.6500469859790545
68-69	5.268335089513697
70-71	2.666904944119817
72-73	1.6106731818726479
74-75	0.8544169395355513
76-77	1.1286248472569422
78-79	1.8357149061773943
80-81	2.2005987150184496
82-83	0.8208211919984096
84-85	1.2733938503130051
86-87	0.4281612362411079
88-89	0.6528596212761851
90-91	0.24280953151013315
92-93	0.3291421908684801
94-95	0.5806382059012467
96-97	0.5844321048310925
98-99	0.4749927126240012
100	0.4182558983111485
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	2912571.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	90.50839703136879
#Duplication Level	Percentage of deduplicated	Percentage of total
1	95.15447136333135	86.12278673462407
2	3.3750814721547244	6.10946427789993
3	0.7195945463403605	1.9538804670534315
4	0.2584406203536622	0.9356418510401004
5	0.14718185692838712	0.666059697134429
6	0.08049562448639246	0.43713179641814287
7	0.05484612104591272	0.34748241494777865
8	0.039454928999831294	0.2856801903008958
9	0.025956858696971764	0.21143823053693928
>10	0.13714030292006293	2.0987989464377947
>50	0.0050208524585351145	0.3025665991897938
>100	0.0021239409163477867	0.3362868655765311
>500	1.1490682044587259E-4	0.06829354429728872
>1k	7.660454695676738E-5	0.12448838454291553
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	3.4333926966930594E-5	0.0	0.0	0.0
3	0.0	3.4333926966930594E-5	0.0	0.0	0.0
4	0.0	3.4333926966930594E-5	0.0	0.0	0.0
5	0.0	3.4333926966930594E-5	0.0	0.0	0.0
6	0.0	3.4333926966930594E-5	0.0	0.0	0.0
7	0.0	3.4333926966930594E-5	0.0	0.0	0.0
8	0.0	3.4333926966930594E-5	0.0	0.0	0.0
9	0.0	3.4333926966930594E-5	0.0	0.0	0.0
10-11	0.0	3.4333926966930594E-5	0.0	0.0	0.0
12-13	0.0	3.4333926966930594E-5	0.0	0.0	0.0
14-15	0.0	6.866785393386119E-5	0.0	3.4333926966930594E-5	0.0
16-17	0.0	6.866785393386119E-5	0.0	6.866785393386119E-5	0.0
18-19	0.0	6.866785393386119E-5	0.0	6.866785393386119E-5	1.7166963483465297E-5
20-21	0.0	8.583481741732647E-5	0.0	8.583481741732647E-5	6.866785393386119E-5
22-23	0.0	1.3733570786772238E-4	0.0	1.2016874438425707E-4	6.866785393386119E-5
24-25	3.4333926966930594E-5	1.3733570786772238E-4	0.0	1.545026713511877E-4	6.866785393386119E-5
26-27	3.4333926966930594E-5	1.3733570786772238E-4	0.0	2.9183837921891006E-4	6.866785393386119E-5
28-29	3.4333926966930594E-5	1.8883659831811825E-4	0.0	7.210124663055424E-4	6.866785393386119E-5
30-31	3.4333926966930594E-5	2.0600356180158354E-4	0.0	0.002386207924201676	6.866785393386119E-5
32-33	3.4333926966930594E-5	2.0600356180158354E-4	0.0	0.006403277379332556	6.866785393386119E-5
34-35	3.4333926966930594E-5	2.0600356180158354E-4	0.0	0.012360213708095012	6.866785393386119E-5
36-37	3.4333926966930594E-5	2.2317052528504882E-4	0.0	0.022248384674571024	6.866785393386119E-5
38-39	3.4333926966930594E-5	2.4033748876851413E-4	0.0	0.03744114735743781	6.866785393386119E-5
40-41	3.4333926966930594E-5	2.4033748876851413E-4	0.0	0.05592996702912993	6.866785393386119E-5
42-43	3.4333926966930594E-5	2.4033748876851413E-4	0.0	0.07548313843679691	6.866785393386119E-5
44-45	3.4333926966930594E-5	2.4033748876851413E-4	0.0	0.1009589122462594	6.866785393386119E-5
46-47	3.4333926966930594E-5	2.4033748876851413E-4	0.0	0.1301427501681504	6.866785393386119E-5
48-49	3.4333926966930594E-5	2.7467141573544475E-4	0.0	0.1624509754440321	6.866785393386119E-5
50-51	3.4333926966930594E-5	2.7467141573544475E-4	0.0	0.19690507115534694	6.866785393386119E-5
52-53	3.4333926966930594E-5	2.7467141573544475E-4	0.0	0.2337625417543469	6.866785393386119E-5
54-55	3.4333926966930594E-5	2.7467141573544475E-4	0.0	0.2771777924040306	6.866785393386119E-5
56-57	3.4333926966930594E-5	2.7467141573544475E-4	0.0	0.32409510360434135	6.866785393386119E-5
58-59	3.4333926966930594E-5	2.7467141573544475E-4	0.0	0.3784800439199594	6.866785393386119E-5
60-61	6.866785393386119E-5	2.7467141573544475E-4	0.0	0.43933692946884384	1.0300178090079177E-4
62-63	6.866785393386119E-5	2.7467141573544475E-4	0.0	0.504537056779045	1.0300178090079177E-4
64-65	6.866785393386119E-5	2.7467141573544475E-4	0.0	0.5765868025191488	1.0300178090079177E-4
66-67	6.866785393386119E-5	2.7467141573544475E-4	0.0	0.6665760250994739	1.0300178090079177E-4
68-69	6.866785393386119E-5	2.7467141573544475E-4	0.0	0.7644448839187097	1.0300178090079177E-4
70-71	6.866785393386119E-5	2.7467141573544475E-4	0.0	0.8784850223393696	1.0300178090079177E-4
72-73	6.866785393386119E-5	2.7467141573544475E-4	0.0	0.997228908754499	1.0300178090079177E-4
74-75	6.866785393386119E-5	2.7467141573544475E-4	0.0	1.1249511170714808	1.0300178090079177E-4
76-77	6.866785393386119E-5	2.9183837921891006E-4	0.0	1.267814587180879	1.0300178090079177E-4
78-79	6.866785393386119E-5	3.090053427023753E-4	0.0	1.432068093790675	1.3733570786772238E-4
80-81	1.0300178090079177E-4	3.090053427023753E-4	0.0	1.6162181110778073	1.545026713511877E-4
82-83	1.0300178090079177E-4	3.090053427023753E-4	0.0	1.819835464955189	1.7166963483465297E-4
84-85	1.0300178090079177E-4	3.090053427023753E-4	0.0	2.0322938050265558	1.7166963483465297E-4
86-87	1.0300178090079177E-4	3.090053427023753E-4	0.0	2.2648889932640266	1.7166963483465297E-4
88	1.0300178090079177E-4	3.090053427023753E-4	0.0	2.4567641441187185	1.7166963483465297E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTCGTAC	185	1.9473657E-5	23.476303	1
GTATAGG	745	0.0	19.987494	1
GTATTAG	1045	0.0	17.811821	1
GTACGAC	190	0.0012242463	17.740585	3
GTACATG	9260	0.0	16.884684	1
GTATAGA	975	0.0	15.90885	1
TACATGG	9400	0.0	15.639964	2
GTATTAA	1715	0.0	15.556351	1
TCGTACC	245	0.0034512177	15.191494	2
CATGGGG	4420	0.0	15.125333	4
TACGGGT	225	0.0037866796	14.981282	4
TACCGTC	235	6.5886707E-4	14.829144	7
CGTACCC	270	7.5504556E-4	14.564801	3
ACATGGG	9230	0.0	14.546767	3
TATACTG	1325	0.0	14.356411	5
GTCTAGG	875	0.0	14.181603	1
GTATATA	1325	0.0	14.047813	1
GCGTGCG	325	3.2493062E-5	13.89933	9
ATACCGT	335	4.3712047E-5	13.46785	6
TACACTG	1750	0.0	13.158245	5
>>END_MODULE