FastQCFastQC Report
Wed 25 May 2016
SRR1295085_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295085_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2912571
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT51420.1765450524639571No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA40100.13767904713739168No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA94350.019.95371
GTACTAG5800.018.8915771
GTACCGT2302.294977E-618.3781666
TCAACGC102550.017.7490524
CAACGCA102850.017.743015
ATCAACG104050.017.5374763
CGTACAC2152.6101048E-517.4996363
AACGCAG104350.017.463326
TATCAAC108000.017.3291232
CTAATAC11700.017.2846263
CATGGGG53450.017.2467884
GAGTACT38950.016.39342512-13
ACGCAGA113350.016.072317
TCTAACG2053.596413E-416.0591143
TATACTG14100.016.011135
CGCAGAG115350.015.712238
TAATACT17100.015.402524
AGTACTT45600.015.0838112-13
ACATGGG116650.014.9980783
TACATGG121500.014.8301252
Produced by FastQC (version 0.11.5)