##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1295085_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2912571 Sequences flagged as poor quality 0 Sequence length 100 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.539349598687895 33.0 33.0 33.0 27.0 33.0 2 30.18196088610372 33.0 33.0 33.0 27.0 33.0 3 31.017697422655104 33.0 33.0 33.0 27.0 33.0 4 35.362712531299664 37.0 37.0 37.0 33.0 37.0 5 35.63657332301942 37.0 37.0 37.0 33.0 37.0 6 35.41174172234771 37.0 37.0 37.0 33.0 37.0 7 35.344735287139784 37.0 37.0 37.0 33.0 37.0 8 35.337559153064426 37.0 37.0 37.0 33.0 37.0 9 35.336222533287604 37.0 37.0 37.0 33.0 37.0 10-11 35.30044640971842 37.0 37.0 37.0 33.0 37.0 12-13 35.214597858730315 37.0 37.0 37.0 33.0 37.0 14-15 36.72381394307641 40.0 37.0 40.0 33.0 40.0 16-17 36.708642639097896 40.0 37.0 40.0 33.0 40.0 18-19 36.72902548985072 40.0 37.0 40.0 33.0 40.0 20-21 36.72719995495389 40.0 37.0 40.0 33.0 40.0 22-23 36.69448161092039 40.0 37.0 40.0 33.0 40.0 24-25 36.65451485989526 40.0 37.0 40.0 33.0 40.0 26-27 36.632168108519934 40.0 37.0 40.0 33.0 40.0 28-29 36.577841535880154 40.0 37.0 40.0 33.0 40.0 30-31 36.49485677774035 40.0 37.0 40.0 33.0 40.0 32-33 36.45150023810578 40.0 37.0 40.0 33.0 40.0 34-35 36.33530736246429 37.0 37.0 40.0 33.0 40.0 36-37 36.332167696512805 37.0 37.0 40.0 33.0 40.0 38-39 36.26648843925179 37.0 37.0 40.0 33.0 40.0 40-41 36.198203408603604 37.0 37.0 40.0 30.0 40.0 42-43 36.10987405972249 37.0 37.0 40.0 27.0 40.0 44-45 35.99691509666202 37.0 37.0 40.0 27.0 40.0 46-47 35.88282843577032 37.0 37.0 40.0 27.0 40.0 48-49 35.768023852465745 37.0 37.0 40.0 27.0 40.0 50-51 35.65519158159578 37.0 37.0 40.0 27.0 40.0 52-53 35.548068699441146 37.0 37.0 40.0 27.0 40.0 54-55 35.40980906559874 37.0 33.0 40.0 27.0 40.0 56-57 35.27168728247311 37.0 33.0 40.0 27.0 40.0 58-59 35.12119017871152 37.0 33.0 40.0 27.0 40.0 60-61 34.95021941096028 37.0 33.0 40.0 27.0 40.0 62-63 34.73976943394685 37.0 33.0 40.0 27.0 40.0 64-65 34.55708375864485 37.0 33.0 40.0 27.0 40.0 66-67 34.325670515843214 37.0 33.0 38.5 27.0 40.0 68-69 34.100768015612324 37.0 33.0 37.0 27.0 40.0 70-71 33.86990995240974 37.0 33.0 37.0 27.0 40.0 72-73 33.65490248993072 37.0 33.0 37.0 27.0 40.0 74-75 33.42160637457421 37.0 33.0 37.0 27.0 40.0 76-77 33.21043006333579 37.0 33.0 37.0 27.0 37.0 78-79 32.97541261655081 37.0 33.0 37.0 24.5 37.0 80-81 32.779753351935454 37.0 33.0 37.0 22.0 37.0 82-83 32.5902108480789 37.0 33.0 37.0 22.0 37.0 84-85 32.42637295365503 33.0 33.0 37.0 22.0 37.0 86-87 32.472725128417466 33.0 33.0 37.0 22.0 37.0 88-89 32.508373014769425 33.0 33.0 37.0 22.0 37.0 90-91 32.53415676390378 33.0 33.0 37.0 22.0 37.0 92-93 32.51735253835872 33.0 33.0 37.0 22.0 37.0 94-95 32.56320378112671 33.0 33.0 37.0 22.0 37.0 96-97 32.53442783025719 33.0 33.0 37.0 22.0 37.0 98-99 32.47093478579578 33.0 33.0 37.0 22.0 37.0 100 31.93379388862967 33.0 33.0 37.0 22.0 37.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.5518104046123149 1101 2 4.107421455165198 1101 3 2.3241643580467937 1101 4 1.1549825201564659 1101 5 0.49946926119939405 1101 6 0.5057794573563825 1101 7 0.6078483569835171 1101 8 0.6041249747013993 1101 9 0.709852193507281 1101 10-11 0.6512750381470838 1101 12-13 0.7044750894315399 1101 14-15 1.03038968762543 1101 16-17 0.9658267525744719 1101 18-19 1.0236463286132533 1101 20-21 1.065280928279968 1101 22-23 0.983132641055839 1101 24-25 0.9551967988578198 1101 26-27 1.033726306321043 1101 28-29 0.9111640664935123 1101 30-31 0.8681529531141265 1101 32-33 1.0588685692736917 1101 34-35 1.1250390502110292 1101 36-37 1.0854701490929344 1101 38-39 1.074447237837596 1101 40-41 1.0272906121446823 1101 42-43 1.0509154149674984 1101 44-45 1.1192709396934077 1101 46-47 1.0867338902263342 1101 48-49 0.9727053094713654 1101 50-51 0.9937695911192108 1101 52-53 0.9843563228874217 1101 54-55 0.944855988769298 1101 56-57 0.9565300461616957 1101 58-59 0.9811638291789606 1101 60-61 0.9845823331608514 1101 62-63 1.0596685340648548 1101 64-65 0.9846321641778246 1101 66-67 0.9556707089167276 1101 68-69 1.008368051917408 1101 70-71 0.9366997184288834 1101 72-73 0.9557988055485893 1101 74-75 0.9145142016754306 1101 76-77 0.9343658368676131 1101 78-79 0.9713790592703191 1101 80-81 0.9455033954789229 1101 82-83 0.9410420886168893 1101 84-85 0.9146033641496842 1101 86-87 1.0055698655093863 1101 88-89 0.9501417977784641 1101 90-91 0.937717700581203 1101 92-93 1.0382276981732943 1101 94-95 0.9924942819928333 1101 96-97 0.913882971436422 1101 98-99 0.8876929364147941 1101 100 0.9981934760547588 1102 1 0.6794100460012693 1102 2 4.209360158797818 1102 3 2.434022538249039 1102 4 1.158481376949375 1102 5 0.49889302867799756 1102 6 0.6076217489311304 1102 7 0.6359797230662281 1102 8 0.6137874837204293 1102 9 0.7352934292380979 1102 10-11 0.7164534965177864 1102 12-13 0.7917173750732331 1102 14-15 1.0189486822482579 1102 16-17 1.0441677962178204 1102 18-19 1.0373229290235457 1102 20-21 1.023407210455808 1102 22-23 1.0154135084438138 1102 24-25 1.0654447336388628 1102 26-27 1.027997230076977 1102 28-29 1.0273725759601078 1102 30-31 0.96387026678849 1102 32-33 1.0904572309369485 1102 34-35 1.2192661331096772 1102 36-37 1.0987802393389998 1102 38-39 1.0629714156124663 1102 40-41 1.0077934759361966 1102 42-43 1.1319413627025057 1102 44-45 1.1310407266132927 1102 46-47 1.058942156398082 1102 48-49 0.9873777115521563 1102 50-51 0.9942253210117471 1102 52-53 0.9902855151246115 1102 54-55 1.0111309911982644 1102 56-57 0.977267050393678 1102 58-59 0.9704731654498815 1102 60-61 1.050093156795569 1102 62-63 1.0828509801218829 1102 64-65 1.0296947717890177 1102 66-67 0.9580107047197899 1102 68-69 0.9031557184210826 1102 70-71 1.0187420493449295 1102 72-73 1.0143428251937436 1102 74-75 0.8763893549790609 1102 76-77 0.9651135434850744 1102 78-79 0.8965968997517209 1102 80-81 0.9386293949389781 1102 82-83 0.8981144829727654 1102 84-85 0.9570154365520054 1102 86-87 1.0205914813217234 1102 88-89 0.9111238080947999 1102 90-91 0.9629742004561734 1102 92-93 1.009443422865452 1102 94-95 0.9224819310955468 1102 96-97 0.9292558168235345 1102 98-99 1.0477033302020544 1102 100 0.9526045591508989 1103 1 0.6009199646334586 1103 2 4.08595757708461 1103 3 2.3643726831539595 1103 4 1.1598845592500027 1103 5 0.5194890019790819 1103 6 0.5353658604924831 1103 7 0.5021064807393572 1103 8 0.5293452173196798 1103 9 0.6642111853329595 1103 10-11 0.6172333204782063 1103 12-13 0.6373483504711004 1103 14-15 0.9014068310980363 1103 16-17 0.9574266981587201 1103 18-19 0.9550031123611902 1103 20-21 0.9136995610119456 1103 22-23 0.8291319532230901 1103 24-25 0.8406146240946484 1103 26-27 0.8946090189822797 1103 28-29 0.9523219293330669 1103 30-31 0.884328903463171 1103 32-33 0.9793943232648701 1103 34-35 1.097272625792904 1103 36-37 0.9301503365057613 1103 38-39 0.8583112452861954 1103 40-41 0.8656294960652176 1103 42-43 0.9406181036339589 1103 44-45 0.979678016915642 1103 46-47 0.8920093041442101 1103 48-49 0.8337485767231243 1103 50-51 0.7874060531270004 1103 52-53 0.8470440690255003 1103 54-55 0.8542931022589855 1103 56-57 0.8175083581301195 1103 58-59 0.9210718277815886 1103 60-61 0.9010190691764564 1103 62-63 0.8999004399995485 1103 64-65 0.8587174275069458 1103 66-67 0.8827627236768976 1103 68-69 0.8295173716050144 1103 70-71 0.8477763876666344 1103 72-73 0.7558968614012898 1103 74-75 0.7300386600774686 1103 76-77 0.8055992970069212 1103 78-79 0.829872216258849 1103 80-81 0.8583648530433337 1103 82-83 0.8294030965179289 1103 84-85 0.9491164471886577 1103 86-87 0.9741854870691569 1103 88-89 0.9259524100751975 1103 90-91 0.9082057222341717 1103 92-93 0.8278659188466335 1103 94-95 0.8025468731660936 1103 96-97 0.8633489917854646 1103 98-99 0.8987429955879023 1103 100 0.8254515060700243 1104 1 0.5025373910481825 1104 2 3.682434685981743 1104 3 2.2027125724927252 1104 4 1.021612615581013 1104 5 0.432275128367003 1104 6 0.39763122842381904 1104 7 0.3932869699335626 1104 8 0.3067636389275705 1104 9 0.4515764750557807 1104 10-11 0.4475566543763705 1104 12-13 0.4834915515035192 1104 14-15 0.6018071185829754 1104 16-17 0.6149647314990787 1104 18-19 0.5653160602187342 1104 20-21 0.5995436789455013 1104 22-23 0.5114517549486877 1104 24-25 0.586469134333008 1104 26-27 0.6167696171547448 1104 28-29 0.6613592932916532 1104 30-31 0.5545530343865792 1104 32-33 0.7219464526172246 1104 34-35 0.772150716665756 1104 36-37 0.525284845023549 1104 38-39 0.5694692230401515 1104 40-41 0.6593918110873673 1104 42-43 0.6985096622197133 1104 44-45 0.7350709695464985 1104 46-47 0.708437785114775 1104 48-49 0.5820577652265229 1104 50-51 0.5738722999044583 1104 52-53 0.5193501062195907 1104 54-55 0.5885665194761955 1104 56-57 0.5771906682745396 1104 58-59 0.6481731295759445 1104 60-61 0.6211053564185463 1104 62-63 0.6568996896530095 1104 64-65 0.6119155094929809 1104 66-67 0.5987614910914942 1104 68-69 0.6002632214954033 1104 70-71 0.5301292330377194 1104 72-73 0.5252821535441754 1104 74-75 0.608861008565043 1104 76-77 0.5728330458758464 1104 78-79 0.6319538040994601 1104 80-81 0.5240816062551161 1104 82-83 0.6733240993408813 1104 84-85 0.5757957090698369 1104 86-87 0.5982316193391668 1104 88-89 0.5469729085153574 1104 90-91 0.5155323963072078 1104 92-93 0.6356601422327444 1104 94-95 0.5492950495796975 1104 96-97 0.5448124515167407 1104 98-99 0.5838695757188503 1104 100 0.5831666752748959 1105 1 0.4864203687651383 1105 2 3.7856528844859625 1105 3 2.1945995974717825 1105 4 0.9754829641592551 1105 5 0.4648701921414897 1105 6 0.4006630684676864 1105 7 0.34712893666292644 1105 8 0.32340334719352626 1105 9 0.38113852796979586 1105 10-11 0.35595685057177917 1105 12-13 0.4040918396289399 1105 14-15 0.5476963396794545 1105 16-17 0.5246052615319314 1105 18-19 0.4850454750937274 1105 20-21 0.45749184584916947 1105 22-23 0.4867986694758386 1105 24-25 0.5574374561127158 1105 26-27 0.5001743685584898 1105 28-29 0.49385006553196575 1105 30-31 0.5213881122192277 1105 32-33 0.6322170544681853 1105 34-35 0.6540067233678357 1105 36-37 0.5028849020172359 1105 38-39 0.5330953225493218 1105 40-41 0.45080375109597526 1105 42-43 0.5972033967723789 1105 44-45 0.4895493169137808 1105 46-47 0.5651700276163965 1105 48-49 0.5109416853463031 1105 50-51 0.4779282438215162 1105 52-53 0.5191622528773365 1105 54-55 0.5043587499163209 1105 56-57 0.42286940895257175 1105 58-59 0.47769528064201694 1105 60-61 0.4687377666291894 1105 62-63 0.5386231693241328 1105 64-65 0.46113827020953835 1105 66-67 0.41557356551403757 1105 68-69 0.41434028924376065 1105 70-71 0.49988045437192596 1105 72-73 0.4237724325779979 1105 74-75 0.4726209470469982 1105 76-77 0.45120127355117035 1105 78-79 0.3422212802882356 1105 80-81 0.4538339430012073 1105 82-83 0.49750999240163907 1105 84-85 0.4456246067679501 1105 86-87 0.49286591320555573 1105 88-89 0.42687234477762104 1105 90-91 0.3893333938638648 1105 92-93 0.4179303828031564 1105 94-95 0.4454790551168202 1105 96-97 0.5021594720446032 1105 98-99 0.44614333501128556 1105 100 0.372270825997461 1106 1 0.44159371214391996 1106 2 3.8327417884834496 1106 3 2.1973397111298816 1106 4 0.9131827835887592 1106 5 0.31299769860875415 1106 6 0.178260376812716 1106 7 0.0934430070381751 1106 8 0.1490432708824727 1106 9 0.2340085932626863 1106 10-11 0.25749965831030863 1106 12-13 0.2764806859459412 1106 14-15 0.28578741623280735 1106 16-17 0.31978036336970206 1106 18-19 0.33909449112728396 1106 20-21 0.22920486029000386 1106 22-23 0.21107693701981844 1106 24-25 0.23650623586092223 1106 26-27 0.24481295091716504 1106 28-29 0.23003668805525024 1106 30-31 0.22316737710757906 1106 32-33 0.27482356819439246 1106 34-35 0.43186043575014565 1106 36-37 0.31344222487459206 1106 38-39 0.2146251869180631 1106 40-41 0.19948719336028375 1106 42-43 0.28836974269714943 1106 44-45 0.34637148087441005 1106 46-47 0.2905912050714434 1106 48-49 0.15525703717327133 1106 50-51 0.24378380864939686 1106 52-53 0.12410967470107437 1106 54-55 0.12665635090834115 1106 56-57 0.20073370746007413 1106 58-59 0.152917911402767 1106 60-61 0.16437615671956962 1106 62-63 0.29142159509910215 1106 64-65 0.18782064931358633 1106 66-67 0.07859154348327024 1106 68-69 0.10711960440989543 1106 70-71 0.11234117012030964 1106 72-73 0.16938044124699303 1106 74-75 0.2417092214353218 1106 76-77 0.20890410712085838 1106 78-79 0.26051026432592295 1106 80-81 0.23597355807554266 1106 82-83 0.21281659463189584 1106 84-85 0.2482679041210929 1106 86-87 0.30062714732036966 1106 88-89 0.27105452365483984 1106 90-91 0.2804246515688078 1106 92-93 0.33212339540468605 1106 94-95 0.31349084184505216 1106 96-97 0.1608060877311246 1106 98-99 0.22081453981592603 1106 100 0.2788432938354717 1107 1 0.4211116553763681 1107 2 3.509156333033662 1107 3 2.096488842058399 1107 4 0.9570093198572494 1107 5 0.31794830490633785 1107 6 0.1993039367548235 1107 7 0.09552961755452571 1107 8 0.15909639495549754 1107 9 0.13457077060061096 1107 10-11 0.1985588288796336 1107 12-13 0.16264880131476644 1107 14-15 0.2383729076379737 1107 16-17 0.1881389928105648 1107 18-19 0.22489219118151738 1107 20-21 0.26975481279894353 1107 22-23 0.20457459392759603 1107 24-25 0.26976357600181444 1107 26-27 0.15860816855207815 1107 28-29 0.11480082076655407 1107 30-31 0.1299647415782701 1107 32-33 0.1957017333913882 1107 34-35 0.41240883768335124 1107 36-37 0.27248631366041565 1107 38-39 0.1513839068466325 1107 40-41 0.23744266561920568 1107 42-43 0.36137582964324366 1107 44-45 0.4154532628829486 1107 46-47 0.2536487381728634 1107 48-49 0.1743137627767979 1107 50-51 0.19002190217533155 1107 52-53 0.19508285947876658 1107 54-55 0.22439711711746924 1107 56-57 0.150822135221226 1107 58-59 0.2554680466848822 1107 60-61 0.29896485714234444 1107 62-63 0.3017489538468965 1107 64-65 0.2602446711025479 1107 66-67 0.2112147580739645 1107 68-69 0.26235811591711666 1107 70-71 0.2701373450672051 1107 72-73 0.2886971691876212 1107 74-75 0.28142751852863057 1107 76-77 0.19823438574508145 1107 78-79 0.22706786634181952 1107 80-81 0.3425143284794743 1107 82-83 0.2337924760630372 1107 84-85 0.2628169113031831 1107 86-87 0.2558735240668213 1107 88-89 0.23849707744558657 1107 90-91 0.22894179179778007 1107 92-93 0.3488327216308349 1107 94-95 0.22966342167348586 1107 96-97 0.2425595192226382 1107 98-99 0.20591388153285095 1107 100 0.3157103667979264 1108 1 0.4466444295390488 1108 2 3.6370751722206904 1108 3 2.1260594887625928 1108 4 0.9658270832141724 1108 5 0.2629521506875463 1108 6 0.12361209159052322 1108 7 -0.008599703511855239 1108 8 0.02538896300802662 1108 9 0.03676163009811262 1108 10-11 0.011308779942318381 1108 12-13 0.03206119758247894 1108 14-15 -0.0452945835483618 1108 16-17 0.012149396192484119 1108 18-19 0.09431086526688404 1108 20-21 0.04305001456899049 1108 22-23 -0.06072951443446328 1108 24-25 0.008619782341320104 1108 26-27 -0.022136899653695252 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0.3213585973744699 2211 34-35 0.37255930373172674 2211 36-37 0.1085463106672222 2211 38-39 0.04427386283744994 2211 40-41 0.09611483694168754 2211 42-43 0.1492320889092582 2211 44-45 0.20314992454537872 2211 46-47 0.31416949404582084 2211 48-49 0.17161954476998176 2211 50-51 0.10328136461786386 2211 52-53 0.2551872585265613 2211 54-55 0.2142205776144337 2211 56-57 0.10941532619115435 2211 58-59 0.09596398016132213 2211 60-61 0.19796436139594675 2211 62-63 0.29907180635299113 2211 64-65 0.17950628957424186 2211 66-67 0.16705123659085075 2211 68-69 0.19885656787881345 2211 70-71 0.25413221923647455 2211 72-73 0.2508611957708524 2211 74-75 0.1340649712339541 2211 76-77 0.04921085415995918 2211 78-79 0.032931236762600236 2211 80-81 0.009838268353853152 2211 82-83 -0.4450762750769428 2211 84-85 -0.07253980395599058 2211 86-87 -0.02430299205195041 2211 88-89 0.17094882401627842 2211 90-91 0.21772295717597956 2211 92-93 0.2566038387906744 2211 94-95 0.23263556166104848 2211 96-97 0.12227922373907774 2211 98-99 0.10115120843083503 2211 100 -0.042060808822576234 2212 1 0.5525680782497027 2212 2 4.0326592779378565 2212 3 2.17140683206809 2212 4 0.990557743028802 2212 5 0.5196824434286711 2212 6 0.38457382417747255 2212 7 0.36114059198231985 2212 8 0.2419302128933225 2212 9 0.13364517602818182 2212 10-11 0.2641783770791406 2212 12-13 0.20705385611235272 2212 14-15 0.3865742794414473 2212 16-17 0.3275889667164762 2212 18-19 0.38226488931020697 2212 20-21 0.2606360474220679 2212 22-23 0.273068778673732 2212 24-25 0.45007018153375355 2212 26-27 0.29399827516506605 2212 28-29 0.18693419883260987 2212 30-31 0.20123440533730985 2212 32-33 0.41278143219105345 2212 34-35 0.4331247476214699 2212 36-37 0.3356669965799668 2212 38-39 0.3299858228982302 2212 40-41 0.45335707701114103 2212 42-43 0.45911813188732964 2212 44-45 0.6591494904233883 2212 46-47 0.5241998825850018 2212 48-49 0.32139271603110586 2212 50-51 0.3083160943769272 2212 52-53 0.3330372693796093 2212 54-55 0.402074929687366 2212 56-57 0.5673760381512167 2212 58-59 0.2992285775131833 2212 60-61 0.3433941421643425 2212 62-63 0.2754110726868291 2212 64-65 0.4965065066352352 2212 66-67 0.4535088011664925 2212 68-69 0.3363473427865671 2212 70-71 0.5546303742180285 2212 72-73 0.5096261187142019 2212 74-75 0.4091474544117233 2212 76-77 0.31304632192640724 2212 78-79 0.17583659816915542 2212 80-81 0.1711948995672259 2212 82-83 0.46025691354906684 2212 84-85 0.5787907799380889 2212 86-87 0.36297614838651526 2212 88-89 0.5184862670377726 2212 90-91 0.5150802395723275 2212 92-93 0.5623060311067221 2212 94-95 0.5411779970853985 2212 96-97 0.34381818618752646 2212 98-99 0.5256812713785237 2212 100 0.3439691455946132 2213 1 -0.5161979704204676 2213 2 -26.001458369120964 2213 3 -14.923265598377856 2213 4 -5.983187928247322 2213 5 -2.0667042794623285 2213 6 -1.307084400986838 2213 7 -1.1537530450880453 2213 8 -1.2388031377156494 2213 9 -1.2276255252839832 2213 10-11 -1.1842524457906478 2213 12-13 -1.0982281293290015 2213 14-15 -1.4987345617562298 2213 16-17 -1.517569105322906 2213 18-19 -1.4929311105955634 2213 20-21 -1.3503499593154302 2213 22-23 -1.4688438694451875 2213 24-25 -1.4861496351874806 2213 26-27 -1.5140602185568142 2213 28-29 -1.3803335813271076 2213 30-31 -1.4432359002058135 2213 32-33 -1.339091285658597 2213 34-35 -1.2123504108039427 2213 36-37 -1.2989443016838322 2213 38-39 -1.4103749629860118 2213 40-41 -1.345701911422033 2213 42-43 -1.3680677853309717 2213 44-45 -1.4114759660066767 2213 46-47 -1.432602868952138 2213 48-49 -1.294607307526583 2213 50-51 -1.4791704188507069 2213 52-53 -1.360282596812766 2213 54-55 -1.3241934158567048 2213 56-57 -1.31101391214208 2213 58-59 -1.188864899364951 2213 60-61 -1.3410864109700071 2213 62-63 -1.3723465894489166 2213 64-65 -1.366895222498549 2213 66-67 -1.1902276826140508 2213 68-69 -1.3814620284589338 2213 70-71 -1.2493123570595088 2213 72-73 -1.4036900960376215 2213 74-75 -1.4334114986849293 2213 76-77 -1.4004494023545 2213 78-79 -1.4598781418718367 2213 80-81 -1.4494720183813712 2213 82-83 -1.4668318282352004 2213 84-85 -1.36549082858037 2213 86-87 -1.1882174015204328 2213 88-89 -1.1930236625247623 2213 90-91 -1.1455695990575592 2213 92-93 -1.3705896790395755 2213 94-95 -1.3897611298669403 2213 96-97 -1.4631798585249527 2213 98-99 -1.4011290186165297 2213 100 -1.3587253819560914 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3.0 3 13.0 4 27.0 5 62.0 6 37.0 7 38.0 8 39.0 9 84.0 10 338.0 11 1479.0 12 4363.0 13 7749.0 14 10461.0 15 11798.0 16 13037.0 17 13869.0 18 14499.0 19 16011.0 20 17609.0 21 20065.0 22 22370.0 23 24456.0 24 25569.0 25 27257.0 26 29863.0 27 34060.0 28 40591.0 29 48852.0 30 59477.0 31 74027.0 32 95521.0 33 129044.0 34 179078.0 35 260290.0 36 407196.0 37 700126.0 38 620875.0 39 2338.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 39.22689756548568 19.683604902617695 10.393113203790369 30.69638432810625 2 13.483010502598395 21.827815532216448 35.79539920776247 28.893774757422687 3 15.924762005801746 25.745054798664135 28.73162576980956 29.598557425724557 4 11.071083251843143 18.164781404854253 36.37601908518563 34.38811625811697 5 12.12526188507733 38.118568081866336 34.81943513985317 14.936734893203162 6 30.97734023925679 38.36023595200299 17.381277673706453 13.281146135033776 7 27.020896666583088 32.68615639201145 22.1556666164065 18.13728032499896 8 25.437205075061808 35.933596076575206 20.190435037683756 18.43876381067923 9 25.303283072100633 16.540798910760465 20.915583592573853 37.24033442456505 10-11 23.63286009804755 26.89041224108809 28.760717778825406 20.716009882038954 12-13 24.775739065427878 24.23124091823288 28.626794735790885 22.366225280548356 14-15 22.072199874489364 25.99143614878129 25.78992871586542 26.14643526086394 16-17 21.714615922255405 29.098102923321832 26.74661739224512 22.440663762177646 18-19 21.83643422194106 28.266493712020214 28.433764483577896 21.463307582460832 20-21 22.577663518841362 27.593573209560958 28.08709901028691 21.741664261310767 22-23 22.34677295280588 27.348939931755616 27.93022945937481 22.374057656063695 24-25 22.212471402874854 27.550956282370297 27.847384243026973 22.389188071727876 26-27 22.067130322750785 27.369415592799434 28.050134330695254 22.51331975375453 28-29 22.003987969128524 27.287746584530918 28.221792621827856 22.486472824512703 30-31 22.338070700425966 27.59646403428474 27.9023005824992 22.163164682790097 32-33 21.90893534267872 27.52317234124079 28.197947896738363 22.369944419342126 34-35 21.8982444219396 27.41967742384057 28.16430580408071 22.51777235013912 36-37 22.352523294018116 27.454271208054543 27.89209951920089 22.30110597872645 38-39 22.306904689390098 27.542084952425554 27.884776830519886 22.266233527664458 40-41 22.143738972681867 27.395167405784193 27.98403499281255 22.477058628721387 42-43 22.123749014757646 27.33528833800968 27.93638274461437 22.604579902618312 44-45 22.512898175533305 27.34842422589057 27.757066033542554 22.38161156503357 46-47 22.30137500925023 27.516135750635414 27.79960918096782 22.382880059146533 48-49 22.34432888575075 27.532639111161174 27.694031685763388 22.42900031732469 50-51 22.297236852068743 27.480772767256862 27.67789150261998 22.544098878054417 52-53 22.245636689585066 27.510199946424525 27.34882099609174 22.895342367898674 54-55 22.356820005456946 27.757495959233285 27.365138498749346 22.52054553656042 56-57 22.38711613717623 27.322424168334415 27.416977667037074 22.87348202745228 58-59 22.225757208696844 27.137696269726984 27.806581335946746 22.829965185629426 60-61 22.440495648686365 27.446000032964495 27.56492975815047 22.548574560198666 62-63 22.101645174732745 27.51191896187632 27.57005647030463 22.816379393086304 64-65 21.949360507363334 27.354484690226222 27.738602662915746 22.957552139494698 66-67 22.20058544639601 27.338368648535045 27.537443775099025 22.923602129969925 68-69 22.480474528667838 27.282290329244358 27.46430998152844 22.772925160559364 70-71 22.272901486927566 27.229137818293715 27.471498494389678 23.02646220038904 72-73 22.316521791688768 27.329814757037425 27.531351325269927 22.822312126003883 74-75 22.381767358224803 27.304952908876455 27.381261666748802 22.93201806614994 76-77 22.283887872034285 27.2772601930612 27.5254375813436 22.913414353560917 78-79 22.397371699064006 27.313932415854623 27.57277919473516 22.715916690346212 80-81 22.21392186188433 27.28177544764858 27.54827596212403 22.95602672834306 82-83 22.160974944795043 27.289224534728046 27.478420192224164 23.07138032825275 84-85 22.094316308659256 27.32897393719331 27.35551695619904 23.221192797948394 86-87 22.113051683300633 27.29233523582965 27.44420759574689 23.15040548512283 88-89 22.11010406015203 27.084104473563308 27.602293539865002 23.203497926419658 90-91 22.08282193474597 27.134521477710578 27.59308484383694 23.189571743706512 92-93 21.877793497617386 27.24093123482917 27.62194993193887 23.25932533561457 94-95 21.81082583441973 27.11104045859738 27.70785773071069 23.3702759762722 96-97 21.928000326862353 27.113369391194837 27.637499351940452 23.321130930002358 98-99 21.93410729349818 27.111884315924893 27.546073839686354 23.407934550890577 100 21.855505979283365 27.011052583461474 27.62021978686709 23.513221650388065 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 8.0 1 8.0 2 10.0 3 12.0 4 18.0 5 38.5 6 100.5 7 218.5 8 364.0 9 432.5 10 350.0 11 296.0 12 297.5 13 338.5 14 444.0 15 516.0 16 602.0 17 682.0 18 790.5 19 983.5 20 1268.5 21 1677.5 22 2314.0 23 3507.0 24 5332.5 25 7691.0 26 11028.5 27 16236.0 28 23662.0 29 32063.0 30 39881.5 31 47908.0 32 56535.5 33 64958.5 34 72716.5 35 79952.5 36 86736.5 37 92516.0 38 97188.0 39 100829.5 40 103045.5 41 104339.5 42 105006.0 43 104419.5 44 102520.0 45 100767.0 46 99880.0 47 99331.0 48 99174.0 49 99600.5 50 104864.0 51 101524.0 52 88173.5 53 81349.5 54 77849.5 55 73990.5 56 70508.0 57 66083.0 58 61096.0 59 57940.5 60 53049.0 61 44454.0 62 36084.5 63 28675.5 64 22529.0 65 18392.5 66 14217.5 67 10256.5 68 7995.0 69 6977.5 70 5920.0 71 4124.5 72 2584.5 73 1837.5 74 1341.5 75 898.5 76 534.5 77 332.0 78 205.5 79 94.5 80 47.5 81 21.5 82 9.5 83 6.0 84 3.0 85 3.5 86 3.0 87 1.5 88 0.5 89 0.5 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content warn #Base N-Count 1 0.8489406781843258 2 5.523333165097092 3 0.0 4 0.005321758679874242 5 0.16504318693003533 6 0.05630764022576617 7 0.03618795902314484 8 0.042471067658093144 9 0.04058270167491196 10-11 0.04217922927887423 12-13 0.04032519722265998 14-15 0.04339808368620027 16-17 0.03385325198939356 18-19 0.028325489747717737 20-21 0.022883562323459238 22-23 0.023450072118413594 24-25 0.019398668736315784 26-27 0.01218854407326036 28-29 0.01931283391889846 30-31 0.024771928306640423 32-33 0.001613694567445738 34-35 0.003828232856812761 36-37 0.004394742651767115 38-39 0.006454778269782952 40-41 0.006918286283836514 42-43 0.007433295188340473 44-45 0.008257309435546808 46-47 0.016531785834577078 48-49 0.02496076490495854 50-51 0.02130420168298043 52-53 0.027158136230842095 54-55 0.02353590693583092 56-57 0.015896608185688863 58-59 0.014128410946891937 60-61 0.011913872657524915 62-63 0.017493135789651136 64-65 0.019742008005985093 66-67 0.02077202581499301 68-69 0.027381306756127148 70-71 0.027295471938709816 72-73 0.023776244424599434 74-75 0.02460025867180577 76-77 0.01857465448910945 78-79 0.014540418070495106 80-81 0.01309839313788402 82-83 0.008308810325997203 84-85 0.010901021812000464 86-87 0.008823819230501162 88-89 0.0074504621518239385 90-91 0.007364627334406612 92-93 0.003965568564680484 94-95 0.0013046892247433625 96-97 0.0010300178090079178 98-99 6.180106854047506E-4 100 0.10386012907496504 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 2912571.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 72.93929913159766 #Duplication Level Percentage of deduplicated Percentage of total 1 85.00464268199283 62.0017906015645 2 9.952985386100115 14.519275566583525 3 2.4757776120682764 5.4174445148988175 4 0.9036555941507146 2.6364802277480237 5 0.469321209937815 1.7115980060228821 6 0.27678302640039815 1.2113015974300523 7 0.18660048909054816 0.9527356224314544 8 0.12366341829621438 0.7215938446994774 9 0.09503645373962946 0.6238703094948967 >10 0.46833363612585444 6.131975753151115 >50 0.028792743153797068 1.4214158975223363 >100 0.013554445633825364 1.7347352464459496 >500 3.792459157016639E-4 0.19366809639581467 >1k 4.2665165474541474E-4 0.544317652420545 >5k 4.740573941615719E-5 0.1777970631906011 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 5142 0.1765450524639571 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 4010 0.13767904713739168 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 3.4333926966930594E-5 0.0 6 0.0 0.0 0.0 3.4333926966930594E-5 0.0 7 0.0 0.0 0.0 6.866785393386119E-5 0.0 8 0.0 0.0 0.0 1.0300178090079177E-4 0.0 9 0.0 0.0 0.0 1.0300178090079177E-4 0.0 10-11 0.0 0.0 0.0 1.0300178090079177E-4 0.0 12-13 0.0 0.0 0.0 1.0300178090079177E-4 0.0 14-15 0.0 0.0 0.0 1.5450267135118766E-4 0.0 16-17 0.0 0.0 0.0 2.0600356180158354E-4 0.0 18-19 0.0 3.4333926966930594E-5 0.0 2.0600356180158354E-4 0.0 20-21 0.0 3.4333926966930594E-5 0.0 2.4033748876851413E-4 0.0 22-23 0.0 3.4333926966930594E-5 0.0 2.5750445225197944E-4 0.0 24-25 0.0 3.4333926966930594E-5 0.0 2.9183837921891006E-4 0.0 26-27 0.0 3.4333926966930594E-5 0.0 4.8067497753702827E-4 0.0 28-29 0.0 3.4333926966930594E-5 0.0 0.0010300178090079178 0.0 30-31 0.0 3.4333926966930594E-5 0.0 0.0032102221714080106 0.0 32-33 0.0 3.4333926966930594E-5 0.0 0.008858153157468093 0.0 34-35 0.0 3.4333926966930594E-5 0.0 0.017561803643585 0.0 36-37 0.0 3.4333926966930594E-5 0.0 0.03189621815227852 0.0 38-39 0.0 3.4333926966930594E-5 0.0 0.05364676088582905 0.0 40-41 0.0 3.4333926966930594E-5 0.0 0.07896803202394037 0.0 42-43 0.0 3.4333926966930594E-5 0.0 0.10492448081093989 0.0 44-45 0.0 6.866785393386119E-5 0.0 0.14037426040429574 0.0 46-47 0.0 6.866785393386119E-5 0.0 0.18087112726179036 0.0 48-49 0.0 6.866785393386119E-5 0.0 0.22454388236372608 0.0 50-51 0.0 6.866785393386119E-5 0.0 0.2709633516230162 0.0 52-53 0.0 6.866785393386119E-5 0.0 0.32076471268854906 0.0 54-55 0.0 6.866785393386119E-5 0.0 0.3780165359059058 0.0 56-57 0.0 6.866785393386119E-5 0.0 0.4428389900194708 0.0 58-59 0.0 6.866785393386119E-5 0.0 0.5188714712877386 0.0 60-61 3.4333926966930594E-5 6.866785393386119E-5 0.0 0.6013758977892727 0.0 62-63 6.866785393386119E-5 6.866785393386119E-5 0.0 0.6897514258021521 0.0 64-65 6.866785393386119E-5 6.866785393386119E-5 0.0 0.7866761016297972 0.0 66-67 1.0300178090079177E-4 6.866785393386119E-5 0.0 0.9026561069240887 0.0 68-69 1.0300178090079177E-4 6.866785393386119E-5 0.0 1.0323010151512186 0.0 70-71 1.2016874438425707E-4 6.866785393386119E-5 0.0 1.1782202047606738 0.0 72-73 1.3733570786772238E-4 6.866785393386119E-5 0.0 1.3274354513589541 0.0 74-75 1.3733570786772238E-4 6.866785393386119E-5 0.0 1.4853886823703182 0.0 76-77 1.3733570786772238E-4 6.866785393386119E-5 0.0 1.6638564347444236 0.0 78-79 1.3733570786772238E-4 8.583481741732647E-5 0.0 1.8668557779364006 0.0 80-81 1.3733570786772238E-4 1.0300178090079177E-4 0.0 2.087777431005115 0.0 82-83 1.3733570786772238E-4 1.0300178090079177E-4 0.0 2.3298144491584925 0.0 84-85 1.3733570786772238E-4 1.0300178090079177E-4 0.0 2.583336165882308 0.0 86-87 1.3733570786772238E-4 1.0300178090079177E-4 0.0 2.861887315365016 0.0 88 1.3733570786772238E-4 1.0300178090079177E-4 0.0 3.088783071725977 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 9435 0.0 19.9537 1 GTACTAG 580 0.0 18.891577 1 GTACCGT 230 2.294977E-6 18.378166 6 TCAACGC 10255 0.0 17.749052 4 CAACGCA 10285 0.0 17.74301 5 ATCAACG 10405 0.0 17.537476 3 CGTACAC 215 2.6101048E-5 17.499636 3 AACGCAG 10435 0.0 17.46332 6 TATCAAC 10800 0.0 17.329123 2 CTAATAC 1170 0.0 17.284626 3 CATGGGG 5345 0.0 17.246788 4 GAGTACT 3895 0.0 16.393425 12-13 ACGCAGA 11335 0.0 16.07231 7 TCTAACG 205 3.596413E-4 16.059114 3 TATACTG 1410 0.0 16.01113 5 CGCAGAG 11535 0.0 15.71223 8 TAATACT 1710 0.0 15.40252 4 AGTACTT 4560 0.0 15.08381 12-13 ACATGGG 11665 0.0 14.998078 3 TACATGG 12150 0.0 14.830125 2 >>END_MODULE