FastQCFastQC Report
Wed 25 May 2016
SRR1295084_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295084_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2704635
Sequences flagged as poor quality0
Sequence length100
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACGGC1450.005283459619.4411013
GTACATG72900.018.3237721
TACATGG73300.017.2014452
GTATTAA15850.016.463571
GTATAGC6051.0913936E-1116.4311731
ACATGGG76200.016.055493
GTATTAG11650.015.9992371
GTATAGA10550.015.9006641
GTATAAT14100.015.8630731
GTGTTAG12450.014.9711731
GTACTAA8750.014.9112881
GTATATA22000.014.6853611
GTGTATA17600.014.4735521
GTCTTGC15600.014.3377781
CATGGGG32400.013.9204424
GTATAGG8601.8189894E-1213.7264791
GTGTAGG8801.8189894E-1213.4145121
TATATAC22750.013.3823143
GTCTTAG10750.013.293011
GTATACA15450.013.2705651
Produced by FastQC (version 0.11.5)