##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1295084_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2704635 Sequences flagged as poor quality 0 Sequence length 100 %GC 42 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 26.277812348061754 33.0 27.0 33.0 6.0 33.0 2 22.404123292052347 27.0 2.0 33.0 2.0 33.0 3 24.741755911610994 27.0 15.0 33.0 15.0 33.0 4 24.692717131886557 33.0 6.0 37.0 2.0 37.0 5 26.63843697948152 33.0 15.0 37.0 2.0 37.0 6 27.732294006400124 33.0 15.0 37.0 2.0 37.0 7 27.56827113455235 33.0 15.0 37.0 6.0 37.0 8 28.275923738323286 33.0 22.0 37.0 6.0 37.0 9 29.341686401307385 33.0 27.0 37.0 15.0 37.0 10-11 28.91147640994071 35.0 24.5 37.0 10.5 37.0 12-13 27.715847055147922 33.0 15.0 37.0 4.0 37.0 14-15 29.46859705653443 33.0 24.5 37.0 6.0 40.0 16-17 29.908455669619006 37.0 27.0 40.0 6.0 40.0 18-19 30.099406389401896 37.0 27.0 40.0 10.5 40.0 20-21 29.616477269576116 33.0 27.0 40.0 6.0 40.0 22-23 30.369050352450515 37.0 27.0 40.0 15.0 40.0 24-25 30.53462666866324 37.0 27.0 40.0 15.0 40.0 26-27 29.10403307655192 33.0 22.0 40.0 4.0 40.0 28-29 29.827317364450288 33.0 27.0 40.0 10.5 40.0 30-31 30.541112571566956 37.0 27.0 40.0 15.0 40.0 32-33 29.34559949863845 33.0 27.0 40.0 6.0 40.0 34-35 28.9338746263359 33.0 22.0 37.0 6.0 40.0 36-37 29.239298463563475 33.0 22.0 37.0 6.0 40.0 38-39 29.360065960841297 33.0 24.5 37.0 6.0 40.0 40-41 28.42233166397684 33.0 22.0 37.0 6.0 40.0 42-43 29.083081635784495 33.0 22.0 37.0 6.0 40.0 44-45 28.971280967672165 33.0 22.0 37.0 6.0 40.0 46-47 28.714589399308963 33.0 22.0 37.0 6.0 40.0 48-49 28.672171845738887 33.0 22.0 37.0 6.0 40.0 50-51 28.66041332749151 33.0 22.0 37.0 6.0 40.0 52-53 28.80026399125945 33.0 22.0 37.0 6.0 40.0 54-55 28.78198832744529 33.0 22.0 37.0 6.0 40.0 56-57 28.786557705568406 33.0 22.0 37.0 6.0 40.0 58-59 28.71885873694602 33.0 22.0 37.0 6.0 40.0 60-61 28.65386882148608 33.0 22.0 37.0 10.5 40.0 62-63 28.62461995796106 33.0 22.0 37.0 10.5 40.0 64-65 28.18554869695911 33.0 22.0 37.0 6.0 40.0 66-67 28.37167048418733 33.0 22.0 37.0 10.5 40.0 68-69 27.939063866288798 33.0 22.0 37.0 6.0 40.0 70-71 28.087369829940084 33.0 22.0 37.0 10.5 37.0 72-73 28.149394465426944 33.0 22.0 37.0 10.5 37.0 74-75 28.23612576188654 33.0 22.0 37.0 15.0 37.0 76-77 28.023822253280017 33.0 22.0 37.0 15.0 37.0 78-79 27.579833138297772 33.0 22.0 37.0 10.5 37.0 80-81 27.352260101640333 33.0 22.0 37.0 10.5 37.0 82-83 27.468429566281586 30.0 22.0 33.0 15.0 37.0 84-85 27.42435707590858 30.0 22.0 33.0 15.0 37.0 86-87 27.199240193223858 27.0 22.0 33.0 15.0 37.0 88-89 27.181684404734835 27.0 22.0 33.0 15.0 37.0 90-91 27.330071155627284 27.0 22.0 33.0 15.0 37.0 92-93 27.243638605578944 27.0 22.0 33.0 15.0 37.0 94-95 27.116519049705413 27.0 22.0 33.0 15.0 37.0 96-97 27.19590832034637 27.0 22.0 33.0 15.0 37.0 98-99 27.268443246500915 27.0 22.0 33.0 15.0 37.0 100 26.947175496878508 27.0 22.0 33.0 15.0 37.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 7.255885820804586 1101 2 11.355285453107935 1101 3 8.493160180481048 1101 4 12.863565114341501 1101 5 10.79624234419699 1101 6 10.016054621912545 1101 7 9.546259815382836 1101 8 9.073034764586563 1101 9 7.446025886623342 1101 10-11 7.796907575342299 1101 12-13 9.16334878050904 1101 14-15 8.122712653407309 1101 16-17 8.279366598502165 1101 18-19 8.164033365485427 1101 20-21 7.379131183481579 1101 22-23 7.54278987286332 1101 24-25 7.4499905690248625 1101 26-27 8.629310858623725 1101 28-29 8.289828415217922 1101 30-31 5.579574130061054 1101 32-33 7.318781060014981 1101 34-35 8.870533954699251 1101 36-37 8.265372269218172 1101 38-39 8.248274127793081 1101 40-41 9.150371598613262 1101 42-43 8.779323712305903 1101 44-45 8.606408575364913 1101 46-47 7.617252359477455 1101 48-49 5.666367248296165 1101 50-51 7.0652372530955 1101 52-53 6.101955171442231 1101 54-55 4.162573824864467 1101 56-57 6.375733547165748 1101 58-59 6.521225079841791 1101 60-61 6.919296472417681 1101 62-63 6.432066377724027 1101 64-65 6.913188939359991 1101 66-67 6.10558300537561 1101 68-69 6.941197235826113 1101 70-71 6.481206959806951 1101 72-73 5.771769765216469 1101 74-75 5.1728383801003694 1101 76-77 5.231747410924978 1101 78-79 5.284739605012685 1101 80-81 5.1227573331544995 1101 82-83 4.706634165624987 1101 84-85 4.397497847131316 1101 86-87 4.215319214727057 1101 88-89 4.229301518891933 1101 90-91 4.095626169976995 1101 92-93 3.9355522270617946 1101 94-95 3.6985554084264756 1101 96-97 3.81579454997728 1101 98-99 3.6935585048211443 1101 100 3.851696547534033 1102 1 6.887675307045509 1102 2 10.513517181123643 1102 3 8.209978881426302 1102 4 12.751896419029325 1102 5 10.628835188785075 1102 6 9.74637074452668 1102 7 6.845856663508346 1102 8 7.904961477098407 1102 9 6.664282105655207 1102 10-11 7.650500162944073 1102 12-13 9.298322408117464 1102 14-15 6.51258929261547 1102 16-17 7.535306023368278 1102 18-19 7.572118891118155 1102 20-21 7.137445986555694 1102 22-23 6.8607694892100035 1102 24-25 7.111812300052243 1102 26-27 8.749964561104264 1102 28-29 7.890595014950332 1102 30-31 4.448472144786592 1102 32-33 4.6976235760066665 1102 34-35 5.292296939687276 1102 36-37 5.376330386340609 1102 38-39 4.736644650506182 1102 40-41 6.555656268017678 1102 42-43 7.290699605222869 1102 44-45 6.797515317463326 1102 46-47 5.817621609776964 1102 48-49 4.070792375813653 1102 50-51 5.449921340341245 1102 52-53 6.133917361319025 1102 54-55 5.602763538195873 1102 56-57 6.55410447487159 1102 58-59 6.303188779237026 1102 60-61 6.274566054001266 1102 62-63 4.475178783830145 1102 64-65 6.331656962236927 1102 66-67 4.053918389444959 1102 68-69 6.271925758876463 1102 70-71 5.593185997382179 1102 72-73 5.2870103198650895 1102 74-75 4.510630094537774 1102 76-77 3.668511975384728 1102 78-79 4.552765913341766 1102 80-81 4.700265670482533 1102 82-83 4.166779624294001 1102 84-85 3.3765828433280625 1102 86-87 3.5868805206887693 1102 88-89 3.5994827862836054 1102 90-91 3.5657180738773704 1102 92-93 3.500153519062305 1102 94-95 3.315859688316234 1102 96-97 3.5884994671047146 1102 98-99 3.4022057038544666 1102 100 3.467264321598151 1103 1 6.7074246504998385 1103 2 10.903640695651589 1103 3 7.900556641616248 1103 4 11.910179866883414 1103 5 10.341995391924499 1103 6 9.179215326076246 1103 7 8.28138342898664 1103 8 8.249334251138773 1103 9 7.199232549947755 1103 10-11 7.528416289984161 1103 12-13 8.840410508152246 1103 14-15 8.727268094884298 1103 16-17 8.183595916762052 1103 18-19 7.39357732188407 1103 20-21 8.554552194060097 1103 22-23 7.107108947305004 1103 24-25 5.348332533051007 1103 26-27 6.125912909846932 1103 28-29 7.104400971228582 1103 30-31 6.231046838098827 1103 32-33 8.079530946106555 1103 34-35 7.276353072843133 1103 36-37 4.232079103228518 1103 38-39 6.459462160789073 1103 40-41 7.000491547004621 1103 42-43 7.580751978980231 1103 44-45 5.877049469829881 1103 46-47 6.578709481969405 1103 48-49 5.800818952422638 1103 50-51 6.599156974282561 1103 52-53 6.30168150852916 1103 54-55 5.199836513696823 1103 56-57 6.016068865513272 1103 58-59 5.488719981644962 1103 60-61 5.248406531390909 1103 62-63 2.844517996095256 1103 64-65 5.230671828949156 1103 66-67 4.276831398642596 1103 68-69 5.414720125174984 1103 70-71 3.987736683307517 1103 72-73 4.537587810241746 1103 74-75 3.4119718910146126 1103 76-77 3.8076225208872394 1103 78-79 3.106181194966883 1103 80-81 4.216295742672578 1103 82-83 3.553340734006081 1103 84-85 3.4751954034364694 1103 86-87 2.9479403622783913 1103 88-89 3.125086418570067 1103 90-91 2.929418548149176 1103 92-93 2.652213783359411 1103 94-95 3.062227896516159 1103 96-97 3.0775569193477637 1103 98-99 2.9968702359982196 1103 100 3.258251358568568 1104 1 6.427015966386168 1104 2 9.884244786851248 1104 3 7.517716144163565 1104 4 12.288000315133221 1104 5 9.569441249391133 1104 6 8.511743704899636 1104 7 4.436041478693163 1104 8 6.7987826559195845 1104 9 6.83000638137948 1104 10-11 7.377091904535813 1104 12-13 7.753121775416837 1104 14-15 7.547700847727025 1104 16-17 7.523377118939372 1104 18-19 7.207098911098175 1104 20-21 7.876117315227024 1104 22-23 6.72726965571017 1104 24-25 6.294636142876087 1104 26-27 8.485668856808559 1104 28-29 7.500756520111832 1104 30-31 6.6284846322990845 1104 32-33 7.898526839409936 1104 34-35 8.222899670290001 1104 36-37 6.98013241764264 1104 38-39 7.700863764725298 1104 40-41 7.463738186099597 1104 42-43 7.650255882279147 1104 44-45 7.0276010650490655 1104 46-47 6.9601224422778 1104 48-49 5.662690477711756 1104 50-51 5.909593335013241 1104 52-53 6.251869409271077 1104 54-55 5.168472828905159 1104 56-57 6.234725520492635 1104 58-59 4.175906894455146 1104 60-61 4.547967652402864 1104 62-63 4.062996799148159 1104 64-65 3.183572546652506 1104 66-67 3.6082007737273436 1104 68-69 4.540472212422916 1104 70-71 3.0921611888573715 1104 72-73 2.9281891410439087 1104 74-75 3.5972101811178625 1104 76-77 4.086582238454991 1104 78-79 4.6734011114769665 1104 80-81 3.355696905913767 1104 82-83 3.2536269436413185 1104 84-85 3.2399153441605613 1104 86-87 1.027791276599526 1104 88-89 0.546115320416142 1104 90-91 2.4642553699146674 1104 92-93 2.763264574673361 1104 94-95 2.753840041296584 1104 96-97 3.102175790781043 1104 98-99 2.7626028067515698 1104 100 0.953844408178238 1105 1 5.813619025558317 1105 2 -2.8763245908880393 1105 3 0.4606802510222465 1105 4 8.346801220812328 1105 5 8.760285205844099 1105 6 8.319143921537318 1105 7 5.428836188248365 1105 8 6.034516480266511 1105 9 5.170767547882715 1105 10-11 5.25309122933248 1105 12-13 5.322422599976104 1105 14-15 6.07010793569194 1105 16-17 6.202305496089345 1105 18-19 6.375721579797617 1105 20-21 7.219563786872062 1105 22-23 2.3768107321703873 1105 24-25 4.314090588123776 1105 26-27 7.069233858848484 1105 28-29 6.141969750756996 1105 30-31 5.451743090831336 1105 32-33 7.216394705934402 1105 34-35 6.3365677660973 1105 36-37 6.471263723218666 1105 38-39 4.374476913773449 1105 40-41 5.570338239066075 1105 42-43 5.7886181384126445 1105 44-45 6.294080649095182 1105 46-47 5.67104856835234 1105 48-49 5.262981912650069 1105 50-51 6.066468062128504 1105 52-53 5.7796009407897095 1105 54-55 5.4619131569156 1105 56-57 5.350701177384362 1105 58-59 2.1944640409933527 1105 60-61 4.696951924698293 1105 62-63 -0.7701493919176841 1105 64-65 -0.21811125657940167 1105 66-67 1.1058566490700166 1105 68-69 3.109674410770456 1105 70-71 3.202526544245753 1105 72-73 2.8933214498814372 1105 74-75 2.838601577166372 1105 76-77 1.9845676631292584 1105 78-79 3.3208177727754062 1105 80-81 1.8107746497325579 1105 82-83 1.1134866122906537 1105 84-85 1.4599152904783175 1105 86-87 -0.09669110727385188 1105 88-89 1.5629990721130973 1105 90-91 1.4214589007748017 1105 92-93 1.7555454556824905 1105 94-95 0.9971938812471492 1105 96-97 2.0378748409632017 1105 98-99 1.9376139905524141 1105 100 2.07430593232451 1106 1 6.232720469495515 1106 2 10.367975156827292 1106 3 6.922242845835594 1106 4 11.92373482284005 1106 5 10.010334612083803 1106 6 9.63989176790929 1106 7 6.953125346382567 1106 8 7.688609524552685 1106 9 7.154311315199987 1106 10-11 6.0397246869782855 1106 12-13 8.289326617287763 1106 14-15 8.299600332325472 1106 16-17 8.053405006276954 1106 18-19 7.445340254529828 1106 20-21 8.411758663498123 1106 22-23 6.53244484384383 1106 24-25 5.392297478080678 1106 26-27 8.013613057936155 1106 28-29 6.709577024866594 1106 30-31 5.755696563966033 1106 32-33 7.748918532491938 1106 34-35 7.156836926805116 1106 36-37 6.361455463109284 1106 38-39 7.43516881953208 1106 40-41 6.982359184478383 1106 42-43 6.600165445034733 1106 44-45 4.754858971502479 1106 46-47 6.431154092288686 1106 48-49 6.208451118455095 1106 50-51 6.854752673944208 1106 52-53 6.002520920389717 1106 54-55 5.601979374262918 1106 56-57 5.262673956140034 1106 58-59 5.1768291123756285 1106 60-61 5.163928162481021 1106 62-63 5.25139270255271 1106 64-65 5.5295893758440755 1106 66-67 3.4581965392075382 1106 68-69 3.709884461073923 1106 70-71 3.3184669332859507 1106 72-73 3.0330618634771866 1106 74-75 3.1634417004635687 1106 76-77 3.792216569521848 1106 78-79 4.485487943330231 1106 80-81 4.03425329761895 1106 82-83 2.4447873835474354 1106 84-85 3.043352866845492 1106 86-87 3.621380213614067 1106 88-89 3.5673777517564282 1106 90-91 3.2998304781973857 1106 92-93 3.1988560161038997 1106 94-95 1.6472807701179342 1106 96-97 1.7461083288312267 1106 98-99 2.1906852347042367 1106 100 3.0950680634157344 1107 1 6.098489043977793 1107 2 10.413782218826537 1107 3 6.201966167562727 1107 4 11.396882637399866 1107 5 9.489135322048874 1107 6 8.280800317503093 1107 7 3.8894413770739398 1107 8 6.415280068640708 1107 9 5.7822687461657445 1107 10-11 4.797369953446751 1107 12-13 6.29880579980545 1107 14-15 6.348819996264911 1107 16-17 7.156287540258013 1107 18-19 6.3706821252072 1107 20-21 5.663428951965042 1107 22-23 5.9964643233752035 1107 24-25 4.788485505750302 1107 26-27 7.28446680837898 1107 28-29 6.653979253401491 1107 30-31 5.281197430972682 1107 32-33 5.234259534057539 1107 34-35 6.823935918936197 1107 36-37 6.668490832650189 1107 38-39 6.671049888353103 1107 40-41 7.824279843295173 1107 42-43 6.375258055058566 1107 44-45 4.415964159889221 1107 46-47 3.63563688958957 1107 48-49 4.286169197844394 1107 50-51 5.157348686736089 1107 52-53 4.336920018317862 1107 54-55 5.560566920282234 1107 56-57 5.579875542554706 1107 58-59 3.1894531713140353 1107 60-61 3.0680030185663334 1107 62-63 0.7018131692025769 1107 64-65 2.3734408762196892 1107 66-67 4.36123597943941 1107 68-69 5.216969090306026 1107 70-71 4.448592783075771 1107 72-73 2.5348144469043987 1107 74-75 2.6477229570608465 1107 76-77 3.2751423876747943 1107 78-79 4.062050344652963 1107 80-81 2.380437175204708 1107 82-83 1.7696662341787004 1107 84-85 2.505055772565811 1107 86-87 2.470229001398625 1107 88-89 2.7261115431226877 1107 90-91 1.5583051299061097 1107 92-93 2.3779386911878184 1107 94-95 2.3803476159399146 1107 96-97 2.3803457088171136 1107 98-99 1.8488924014780643 1107 100 0.07437282813304336 1108 1 4.400643030168673 1108 2 -3.4216639417258747 1108 3 -0.12410451358973518 1108 4 6.4775748166248235 1108 5 6.788627934899061 1108 6 6.811768766194131 1108 7 4.358989471977942 1108 8 5.176888444776669 1108 9 4.961709824740957 1108 10-11 3.238954955129433 1108 12-13 2.594438640762199 1108 14-15 3.9619453251839367 1108 16-17 4.7393364834091045 1108 18-19 4.013424321439132 1108 20-21 4.71198516068036 1108 22-23 4.090363729166938 1108 24-25 2.9139907088627766 1108 26-27 4.611219299457446 1108 28-29 6.072313158637382 1108 30-31 4.632974982190994 1108 32-33 4.5876848101093515 1108 34-35 3.0936135850453717 1108 36-37 1.3309191309293489 1108 38-39 2.530238621302228 1108 40-41 5.365764829577017 1108 42-43 3.2003952291153865 1108 44-45 3.8257258461401698 1108 46-47 4.786473638577181 1108 48-49 4.870101978128439 1108 50-51 2.3301934171418175 1108 52-53 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2213 54-55 -18.37794972032842 2213 56-57 -18.18446615785894 2213 58-59 -18.70561048192649 2213 60-61 -19.976962238142676 2213 62-63 -18.21084284453437 2213 64-65 -18.305843579119966 2213 66-67 -9.08074570091815 2213 68-69 -23.447814500863796 2213 70-71 -18.126985830220175 2213 72-73 -9.8942928233363 2213 74-75 -4.627524554005927 2213 76-77 -3.428806188850924 2213 78-79 -4.943012909517005 2213 80-81 -3.797895418820069 2213 82-83 -3.56244990917666 2213 84-85 -3.4220497909608376 2213 86-87 -3.456057612860736 2213 88-89 -3.109704549111509 2213 90-91 -3.1815310742952505 2213 92-93 -4.098434548136279 2213 94-95 -3.955298778412992 2213 96-97 -3.5959898528955065 2213 98-99 -3.950770870661227 2213 100 -3.4487285639543757 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 730.0 3 724.0 4 3990.0 5 15076.0 6 20079.0 7 24239.0 8 22521.0 9 23004.0 10 25038.0 11 29402.0 12 36536.0 13 50424.0 14 37738.0 15 41560.0 16 48018.0 17 52835.0 18 52580.0 19 52936.0 20 52735.0 21 51139.0 22 52617.0 23 57500.0 24 62932.0 25 70328.0 26 77631.0 27 84156.0 28 91103.0 29 96304.0 30 100921.0 31 109284.0 32 124921.0 33 152422.0 34 189000.0 35 227287.0 36 257937.0 37 232998.0 38 75879.0 39 111.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.46245761969345 21.63649658555139 14.188666202873925 23.71237959188123 2 14.942641212530964 21.31986494046666 41.00960559532352 22.727888251678856 3 17.975913796746248 26.8849260807494 30.20131074124609 24.937849381258257 4 11.93420723529377 18.392806852042597 40.086920815107895 29.586065097555743 5 12.969170849738404 38.087255515817844 36.21887104819111 12.72470258625264 6 29.50443916102271 38.98909369141011 19.16026334936381 12.34620379820337 7 25.46400551938474 32.276875164219426 24.479374039870464 17.779745276525365 8 23.232231860247545 36.92277790273526 22.139478633899554 17.705511603117635 9 24.008817143189088 17.544011140749298 22.709913477072444 35.73725823898917 10-11 22.523192349982338 27.813923867995406 29.988109038623236 19.674774743399023 12-13 23.502155658256505 25.43153494532367 29.755177778209795 21.311131618210027 14-15 20.92643751775244 27.196221497349875 27.06346894637613 24.81387203852156 16-17 20.440436800833847 30.779753319839852 27.799088584275655 20.980721295050643 18-19 20.402008612938 29.813851134537384 29.465873502513347 20.318266750011272 20-21 21.777615916578785 29.13107483066889 28.56957500791229 20.521734244840033 22-23 20.976185729718317 29.09888638757936 28.931092030418093 20.993835852284235 24-25 21.197590050708335 29.013619560867447 28.58055852468019 21.20823186374403 26-27 21.042919344806137 28.914873656140234 28.682570931776986 21.359636067276643 28-29 20.90608493754534 28.878706034067257 29.047587471657625 21.167621556729774 30-31 21.45153228275063 29.268886658736275 28.44435231072656 20.83522874778653 32-33 20.71100018379453 29.018248519478153 28.952537909654417 21.318213387072902 34-35 20.818900904732555 28.561671073988947 28.921405489268842 21.698022532009656 36-37 21.319360279178795 29.157582144189647 28.412837718537858 21.1102198580937 38-39 21.083991804519385 28.868491424127996 28.69130521240086 21.356211558951763 40-41 21.12045878206224 28.938679821241053 28.499922668034223 21.44093872866248 42-43 20.994954506069725 28.87114882213965 28.867460044689736 21.266436627100894 44-45 21.29244955629369 28.639353527341232 28.799504238758615 21.268692677606467 46-47 21.194791240802317 28.936483291938337 28.823405229082528 21.045320238176824 48-49 21.064217154937737 28.714210733954964 28.897970390978077 21.323601720129222 50-51 21.27710241884096 28.725797585256657 28.722214645040488 21.274885350861894 52-53 20.923026504030652 28.704134218252385 28.798038944317277 21.574800333399683 54-55 21.2561535379475 28.883709360094535 28.556340750772634 21.30379635118533 56-57 21.31236578798851 28.785691896463085 28.519191623631485 21.382750691916918 58-59 21.03494216677769 28.326818839318772 28.947676054232684 21.690562939670855 60-61 21.36734530611046 28.819612588459588 28.48239272095138 21.330649384478573 62-63 21.014807846921954 28.86403947130321 28.800208595636747 21.320944086138088 64-65 20.99040026581377 28.433960342475327 28.884689723222962 21.690949668487942 66-67 21.086381629923956 28.595757471887673 28.60193266418316 21.715928234005208 68-69 21.576596571861494 28.615318278421075 28.473920709857232 21.3341644398602 70-71 21.479099507288765 28.64921150232439 28.447533744141495 21.42415524624535 72-73 21.260934549839128 28.73561424094932 28.766289566420923 21.237161642790632 74-75 21.4792350427997 28.46400938124748 28.434459497700544 21.622296078252276 76-77 21.433715086254463 28.79475683592106 28.418259797732677 21.3532682800918 78-79 21.469785020484334 28.60910956974894 28.591079212052644 21.330026197714083 80-81 21.482976221942433 28.51047744641908 28.50670691097463 21.49983942066386 82-83 21.162286246341615 28.655142076385477 28.833487427846915 21.349084249425996 84-85 21.332486696011596 28.432151627046764 28.461145456814936 21.774216220126704 86-87 21.456736474999655 28.68106025752554 28.438586358011904 21.423616909462897 88-89 21.317960172924778 28.299514056423085 28.72130451502012 21.66122125563202 90-91 21.532716103179915 28.330548813163954 28.47497918563447 21.66175589802166 92-93 21.240638412305906 28.642050598321305 28.61335849429183 21.503952495080956 94-95 21.21008900476323 28.286493208608228 28.58382064735641 21.91959713927213 96-97 21.32173943452771 28.422221192943137 28.573822841104175 21.682216531424974 98-99 21.369913810736605 28.294761224362023 28.577983977896547 21.757340987004824 100 21.493145389121835 28.16352930680703 28.365933624765816 21.97739167930532 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 320.0 1 358.5 2 353.0 3 305.5 4 369.0 5 532.5 6 808.0 7 1280.5 8 1841.5 9 2567.5 10 3581.0 11 4578.0 12 5594.5 13 6532.5 14 7381.0 15 8229.0 16 9072.0 17 9987.5 18 10760.5 19 11597.5 20 12469.5 21 13544.5 22 15031.0 23 16765.5 24 19187.0 25 22566.5 26 26953.5 27 32739.5 28 39857.5 29 47868.0 30 56526.0 31 65291.0 32 73719.0 33 81395.0 34 88448.5 35 94976.0 36 99818.0 37 103347.5 38 106099.5 39 107067.5 40 106684.0 41 105145.5 42 101838.5 43 97823.0 44 93467.5 45 88674.0 46 84138.0 47 79607.0 48 75593.5 49 72732.5 50 70081.0 51 65176.5 52 58730.0 53 53012.5 54 48094.0 55 44072.0 56 40124.5 57 35971.5 58 31928.5 59 27679.5 60 23351.0 61 19579.0 62 16049.5 63 12694.0 64 10013.0 65 7866.5 66 6068.5 67 4650.5 68 3581.5 69 2711.5 70 2019.0 71 1537.0 72 1159.0 73 871.5 74 669.0 75 473.0 76 330.0 77 251.5 78 183.0 79 120.0 80 82.5 81 62.0 82 43.0 83 38.5 84 30.0 85 19.0 86 12.0 87 9.5 88 8.5 89 1.5 90 1.0 91 2.5 92 1.5 93 0.0 94 1.0 95 2.0 96 1.0 97 0.0 98 0.0 99 1.0 100 4.0 >>END_MODULE >>Per base N content fail #Base N-Count 1 8.428272206785758 2 25.187539168871215 3 6.377200620416433 4 24.905061126547576 5 12.900446825542078 6 10.409611648152154 7 8.252462901648466 8 9.260658092496769 9 5.800080232637676 10-11 7.107151242219375 12-13 10.613040206904074 14-15 8.034374324077001 16-17 8.480441908057834 18-19 6.92794036903316 20-21 8.527435310125025 22-23 6.152734102753237 24-25 5.670931567475833 26-27 9.946628657841076 28-29 6.603571276715712 30-31 4.578991250205665 32-33 9.072092907176014 34-35 7.677708822077656 36-37 7.4425199703472 38-39 6.56770691793902 40-41 9.158167368240075 42-43 6.783706489045657 44-45 6.775775659192461 46-47 6.1258173468878425 48-49 4.125288624897629 50-51 6.610004677156067 52-53 4.893340506205089 54-55 5.320976767659961 56-57 5.10359068783773 58-59 3.5339703878711917 60-61 4.331601121777985 62-63 3.1506284581838213 64-65 4.524677082120139 66-67 2.7041726517626223 68-69 5.4203062520451 70-71 2.7618144407655745 72-73 1.7062006518439643 74-75 1.0280684824384807 76-77 1.1624489071538302 78-79 1.8774252348283595 80-81 2.431104381922145 82-83 0.9244315776435638 84-85 1.3615885322788472 86-87 0.587583906885772 88-89 0.7266230008855169 90-91 0.30244376782819127 92-93 0.38874007028674845 94-95 0.6232818846165934 96-97 0.6163308542557498 98-99 0.49139717558931245 100 0.5351923642192015 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 2704635.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 96.75164744197009 #Duplication Level Percentage of deduplicated Percentage of total 1 97.71407259836604 94.53997502156182 2 1.934127650068708 3.742600730144275 3 0.22963544073828696 0.6665282160747646 4 0.05249976549682728 0.20317755208540553 5 0.020836530635586117 0.10079843329840185 6 0.014298331859487211 0.08300322978464372 7 0.008046824701013809 0.054498048254987705 8 0.006145218373093246 0.04756480011899478 9 0.003575188058986459 0.03113148011596193 >10 0.015096603880602808 0.21270134257959833 >50 6.249512252955123E-4 0.04210020541644017 >100 9.642624433232477E-4 0.19032076280303353 >500 0.0 0.0 >1k 7.663415279993476E-5 0.08560017776172066 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 3.6973565749167633E-5 0.0 0.0 0.0 3 0.0 3.6973565749167633E-5 0.0 0.0 0.0 4 0.0 3.6973565749167633E-5 0.0 0.0 0.0 5 0.0 3.6973565749167633E-5 0.0 0.0 0.0 6 0.0 3.6973565749167633E-5 0.0 0.0 0.0 7 0.0 3.6973565749167633E-5 0.0 3.6973565749167633E-5 0.0 8 0.0 3.6973565749167633E-5 0.0 3.6973565749167633E-5 0.0 9 3.6973565749167633E-5 3.6973565749167633E-5 0.0 7.394713149833527E-5 0.0 10-11 3.6973565749167633E-5 3.6973565749167633E-5 0.0 9.243391437291908E-5 0.0 12-13 3.6973565749167633E-5 3.6973565749167633E-5 0.0 1.109206972475029E-4 0.0 14-15 3.6973565749167633E-5 5.546034862375145E-5 0.0 1.109206972475029E-4 0.0 16-17 3.6973565749167633E-5 7.394713149833527E-5 0.0 1.2940748012208673E-4 0.0 18-19 3.6973565749167633E-5 7.394713149833527E-5 0.0 1.4789426299667053E-4 0.0 20-21 3.6973565749167633E-5 1.109206972475029E-4 0.0 1.8486782874583817E-4 0.0 22-23 3.6973565749167633E-5 1.109206972475029E-4 0.0 2.7730174311875726E-4 0.0 24-25 3.6973565749167633E-5 1.109206972475029E-4 0.0 3.327620917425087E-4 0.0 26-27 3.6973565749167633E-5 1.109206972475029E-4 0.0 4.621695718645954E-4 0.0 28-29 3.6973565749167633E-5 1.109206972475029E-4 0.0 7.024977492341851E-4 0.0 30-31 3.6973565749167633E-5 1.109206972475029E-4 3.6973565749167633E-5 0.001978085767580468 0.0 32-33 3.6973565749167633E-5 1.109206972475029E-4 3.6973565749167633E-5 0.00523175955350722 0.0 34-35 3.6973565749167633E-5 1.109206972475029E-4 3.6973565749167633E-5 0.01026016449539402 0.0 36-37 3.6973565749167633E-5 1.109206972475029E-4 3.6973565749167633E-5 0.019115333492319666 0.0 38-39 3.6973565749167633E-5 1.109206972475029E-4 3.6973565749167633E-5 0.03344259022012212 0.0 40-41 3.6973565749167633E-5 1.109206972475029E-4 3.6973565749167633E-5 0.05033950976749173 0.0 42-43 5.546034862375145E-5 1.4789426299667053E-4 3.6973565749167633E-5 0.06686669365736966 0.0 44-45 7.394713149833527E-5 1.4789426299667053E-4 3.6973565749167633E-5 0.0885147164035073 0.0 46-47 7.394713149833527E-5 1.4789426299667053E-4 3.6973565749167633E-5 0.11428529173067715 0.0 48-49 7.394713149833527E-5 1.8486782874583817E-4 3.6973565749167633E-5 0.14391960467863502 0.0 50-51 7.394713149833527E-5 2.218413944950058E-4 3.6973565749167633E-5 0.1748849659935629 0.0 52-53 7.394713149833527E-5 2.218413944950058E-4 3.6973565749167633E-5 0.207440190635705 0.0 54-55 7.394713149833527E-5 2.218413944950058E-4 3.6973565749167633E-5 0.24494987308823557 0.0 56-57 7.394713149833527E-5 2.403281773695896E-4 3.6973565749167633E-5 0.2909634756630747 0.0 58-59 7.394713149833527E-5 2.9578852599334107E-4 3.6973565749167633E-5 0.34331804476389605 0.0 60-61 7.394713149833527E-5 2.9578852599334107E-4 3.6973565749167633E-5 0.39607932308795823 0.0 62-63 7.394713149833527E-5 2.9578852599334107E-4 3.6973565749167633E-5 0.45163210562608264 0.0 64-65 7.394713149833527E-5 2.9578852599334107E-4 3.6973565749167633E-5 0.5161694646412547 0.0 66-67 7.394713149833527E-5 3.327620917425087E-4 3.6973565749167633E-5 0.5959769063108331 3.6973565749167633E-5 68-69 7.394713149833527E-5 3.6973565749167633E-4 3.6973565749167633E-5 0.6820328805920207 3.6973565749167633E-5 70-71 7.394713149833527E-5 3.6973565749167633E-4 3.6973565749167633E-5 0.7830631490016213 3.6973565749167633E-5 72-73 7.394713149833527E-5 3.6973565749167633E-4 3.6973565749167633E-5 0.8904158971543294 3.6973565749167633E-5 74-75 7.394713149833527E-5 3.6973565749167633E-4 3.6973565749167633E-5 1.005976776903353 3.6973565749167633E-5 76-77 7.394713149833527E-5 3.6973565749167633E-4 3.6973565749167633E-5 1.1364934639979147 3.6973565749167633E-5 78-79 7.394713149833527E-5 3.6973565749167633E-4 3.6973565749167633E-5 1.2826869429701235 3.6973565749167633E-5 80-81 7.394713149833527E-5 3.6973565749167633E-4 3.6973565749167633E-5 1.447681480125784 3.6973565749167633E-5 82-83 7.394713149833527E-5 3.6973565749167633E-4 3.6973565749167633E-5 1.6304972759725436 3.6973565749167633E-5 84-85 7.394713149833527E-5 3.6973565749167633E-4 3.6973565749167633E-5 1.8177683864920775 3.6973565749167633E-5 86-87 7.394713149833527E-5 3.6973565749167633E-4 3.6973565749167633E-5 2.0225834539595917 5.546034862375145E-5 88 7.394713149833527E-5 3.6973565749167633E-4 3.6973565749167633E-5 2.194565995041845 7.394713149833527E-5 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACGGC 145 0.0052834596 19.441101 3 GTACATG 7290 0.0 18.323772 1 TACATGG 7330 0.0 17.201445 2 GTATTAA 1585 0.0 16.46357 1 GTATAGC 605 1.0913936E-11 16.431173 1 ACATGGG 7620 0.0 16.05549 3 GTATTAG 1165 0.0 15.999237 1 GTATAGA 1055 0.0 15.900664 1 GTATAAT 1410 0.0 15.863073 1 GTGTTAG 1245 0.0 14.971173 1 GTACTAA 875 0.0 14.911288 1 GTATATA 2200 0.0 14.685361 1 GTGTATA 1760 0.0 14.473552 1 GTCTTGC 1560 0.0 14.337778 1 CATGGGG 3240 0.0 13.920442 4 GTATAGG 860 1.8189894E-12 13.726479 1 GTGTAGG 880 1.8189894E-12 13.414512 1 TATATAC 2275 0.0 13.382314 3 GTCTTAG 1075 0.0 13.29301 1 GTATACA 1545 0.0 13.270565 1 >>END_MODULE