FastQCFastQC Report
Wed 25 May 2016
SRR1295084_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295084_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2704635
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT34870.12892682376734754No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA75200.021.3096051
ATCAACG83650.019.1668513
CAACGCA83500.019.1449745
TCAACGC83550.019.1335164
AACGCAG85000.018.782076
TATCAAC88150.018.335592
GAGTACT33700.017.4127412-13
CCTACAC11200.017.2118743
ACGCAGA93600.016.9528987
CGCAGAG94150.016.8538658
GTACATG96900.016.640521
CATGGGG36500.016.617254
TAGGGCG1700.001914686816.5945195
TACATGG99100.015.85656452
TACGACC1800.002804300515.6726014
ACATGGG97400.015.3025093
GCGTCAG3001.6067284E-514.9764691
GCCTTAC7100.014.7655331
GCAGAGT105350.014.7055929
TATACTG16400.014.3346965
Produced by FastQC (version 0.11.5)