##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1295084_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2704635 Sequences flagged as poor quality 0 Sequence length 100 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.32321034076687 33.0 33.0 33.0 27.0 33.0 2 29.920511640202836 33.0 33.0 33.0 27.0 33.0 3 30.778312785274167 33.0 33.0 33.0 27.0 33.0 4 35.116023788792205 37.0 37.0 37.0 33.0 37.0 5 35.413215091870065 37.0 37.0 37.0 33.0 37.0 6 35.129278072641966 37.0 37.0 37.0 33.0 37.0 7 35.039594621825124 37.0 37.0 37.0 33.0 37.0 8 35.02613439521414 37.0 37.0 37.0 33.0 37.0 9 35.013232099710315 37.0 37.0 37.0 33.0 37.0 10-11 34.983594274273614 37.0 37.0 37.0 33.0 37.0 12-13 34.88864412388362 37.0 37.0 37.0 33.0 37.0 14-15 36.32127181671464 37.0 37.0 40.0 27.0 40.0 16-17 36.31807119999556 37.0 37.0 40.0 27.0 40.0 18-19 36.348135885248844 38.5 37.0 40.0 30.0 40.0 20-21 36.34845163950034 40.0 37.0 40.0 27.0 40.0 22-23 36.31154610511215 38.5 37.0 40.0 27.0 40.0 24-25 36.27645227544567 37.0 37.0 40.0 27.0 40.0 26-27 36.25483124340253 37.0 37.0 40.0 27.0 40.0 28-29 36.19930064500386 37.0 37.0 40.0 27.0 40.0 30-31 36.114992041439976 37.0 37.0 40.0 27.0 40.0 32-33 36.071318495841396 37.0 37.0 40.0 27.0 40.0 34-35 35.95040938980675 37.0 37.0 40.0 27.0 40.0 36-37 35.95274593429428 37.0 37.0 40.0 27.0 40.0 38-39 35.8945637766279 37.0 37.0 40.0 27.0 40.0 40-41 35.83786185566629 37.0 37.0 40.0 27.0 40.0 42-43 35.748294871581564 37.0 37.0 40.0 27.0 40.0 44-45 35.63746032274226 37.0 37.0 40.0 27.0 40.0 46-47 35.53434714850617 37.0 37.0 40.0 27.0 40.0 48-49 35.43960497442353 37.0 37.0 40.0 27.0 40.0 50-51 35.34359035507564 37.0 35.0 40.0 27.0 40.0 52-53 35.25377195074381 37.0 33.0 40.0 27.0 40.0 54-55 35.13892318187112 37.0 33.0 40.0 27.0 40.0 56-57 35.01436644870749 37.0 33.0 40.0 27.0 40.0 58-59 34.889910653378365 37.0 33.0 40.0 27.0 40.0 60-61 34.73408537566067 37.0 33.0 40.0 27.0 40.0 62-63 34.553620359124245 37.0 33.0 40.0 27.0 40.0 64-65 34.38468480959538 37.0 33.0 40.0 27.0 40.0 66-67 34.16949403523951 37.0 33.0 40.0 24.5 40.0 68-69 33.956284674272126 37.0 33.0 38.5 22.0 40.0 70-71 33.73100178027719 37.0 33.0 37.0 22.0 40.0 72-73 33.51819339763037 37.0 33.0 37.0 22.0 40.0 74-75 33.281691059976666 37.0 33.0 37.0 22.0 40.0 76-77 33.042364126767566 37.0 33.0 37.0 22.0 38.5 78-79 32.806779288147936 37.0 33.0 37.0 22.0 37.0 80-81 32.586811529097275 37.0 33.0 37.0 22.0 37.0 82-83 32.373454828470386 37.0 33.0 37.0 22.0 37.0 84-85 32.200426859816574 33.0 33.0 37.0 22.0 37.0 86-87 32.217820888955444 33.0 33.0 37.0 22.0 37.0 88-89 32.222076176637515 33.0 33.0 37.0 22.0 37.0 90-91 32.22301327166143 33.0 33.0 37.0 22.0 37.0 92-93 32.186949625365344 33.0 33.0 37.0 22.0 37.0 94-95 32.21206133175087 33.0 33.0 37.0 22.0 37.0 96-97 32.17581355709736 33.0 33.0 37.0 22.0 37.0 98-99 32.094763433882946 33.0 33.0 37.0 22.0 37.0 100 31.565819047671866 33.0 33.0 37.0 22.0 37.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.5990598827396276 1101 2 4.174852967946389 1101 3 2.3995672160027155 1101 4 1.1847880040913665 1101 5 0.480101787455979 1101 6 0.4932667589052926 1101 7 0.5248261083742349 1101 8 0.6171403773257396 1101 9 0.6771469465702893 1101 10-11 0.6384767066670278 1101 12-13 0.636257309647192 1101 14-15 0.9780674954300892 1101 16-17 0.9900476727583438 1101 18-19 0.9877957496282121 1101 20-21 0.9849164980119056 1101 22-23 0.9294913737672772 1101 24-25 0.9496856373372324 1101 26-27 0.9597718488201608 1101 28-29 0.8166624261315079 1101 30-31 0.7206663893751681 1101 32-33 0.9273277378357108 1101 34-35 1.0371679549555282 1101 36-37 0.9193025390308946 1101 38-39 0.9387271116506781 1101 40-41 0.9945419616317537 1101 42-43 1.0620281222284405 1101 44-45 1.0404706766905676 1101 46-47 1.00631241203493 1101 48-49 0.9784148667161787 1101 50-51 0.9422914154429236 1101 52-53 0.8761134203803636 1101 54-55 0.9188925590328978 1101 56-57 0.9436519115350421 1101 58-59 0.9058776988976405 1101 60-61 0.9270337436499432 1101 62-63 0.854360157135531 1101 64-65 0.8483981724743899 1101 66-67 0.9014419198859969 1101 68-69 0.8473991493017223 1101 70-71 0.8205394780966131 1101 72-73 0.7845209600389396 1101 74-75 0.7880323787019492 1101 76-77 0.8409311414264806 1101 78-79 0.8573751221474737 1101 80-81 0.8467723382074723 1101 82-83 0.8289930909429515 1101 84-85 0.8544441525566064 1101 86-87 0.8698288260648859 1101 88-89 0.8195155000288423 1101 90-91 0.8279550134817484 1101 92-93 0.8277044828723952 1101 94-95 0.7277879141217092 1101 96-97 0.6381050899602627 1101 98-99 0.661683428852534 1101 100 0.8038547308543613 1102 1 0.5764623475991435 1102 2 4.092901167974517 1102 3 2.355856245338181 1102 4 1.1201338126741192 1102 5 0.5330136043344567 1102 6 0.595914601508909 1102 7 0.6654315209918948 1102 8 0.5948442451537801 1102 9 0.6645306713107075 1102 10-11 0.654785013657424 1102 12-13 0.7203611380068438 1102 14-15 0.9976189254594914 1102 16-17 0.9831336217925895 1102 18-19 1.0222409426956176 1102 20-21 0.9814288035325447 1102 22-23 1.0291119029234466 1102 24-25 1.0101316889892686 1102 26-27 0.9879732612876069 1102 28-29 0.9238137586681106 1102 30-31 0.9726224822701894 1102 32-33 1.0658003260854727 1102 34-35 1.1512469314873002 1102 36-37 1.0808943372649793 1102 38-39 0.9938324281746063 1102 40-41 0.9712311238048414 1102 42-43 1.1111179562476963 1102 44-45 1.1485404444235456 1102 46-47 1.076416023907548 1102 48-49 1.0937676853552745 1102 50-51 1.016292986814797 1102 52-53 1.0136361901773228 1102 54-55 0.9714364657696919 1102 56-57 0.9568579004205731 1102 58-59 1.0233861687687593 1102 60-61 0.9876225883823508 1102 62-63 1.0194024641509571 1102 64-65 1.0243485542352744 1102 66-67 1.0726647844512698 1102 68-69 1.0033272049168005 1102 70-71 0.9738793649490063 1102 72-73 1.045632554686847 1102 74-75 0.9221068929499623 1102 76-77 0.9374285137598761 1102 78-79 0.8400546867287204 1102 80-81 0.8907511048057302 1102 82-83 0.9624474213831675 1102 84-85 0.9432016863422028 1102 86-87 0.9720398803051999 1102 88-89 0.9281392389366374 1102 90-91 0.9443715050811008 1102 92-93 0.9520071405820936 1102 94-95 0.9813345387280386 1102 96-97 0.9760300691031318 1102 98-99 0.9376805688673713 1102 100 1.022943821159643 1103 1 0.45531591093331514 1103 2 4.053796708260638 1103 3 2.3061844881941234 1103 4 1.0625299045785184 1103 5 0.43472215201945374 1103 6 0.40399401055785233 1103 7 0.3864245238339308 1103 8 0.38735449805876954 1103 9 0.44179896165378807 1103 10-11 0.5451155649159531 1103 12-13 0.5539123605608296 1103 14-15 0.7641429658235595 1103 16-17 0.7280148739660817 1103 18-19 0.7526589134598467 1103 20-21 0.7466509498073179 1103 22-23 0.6621413665346267 1103 24-25 0.748697001792813 1103 26-27 0.7113939541681304 1103 28-29 0.720490753187903 1103 30-31 0.6887967546736888 1103 32-33 0.8202416476909207 1103 34-35 0.8754044694710146 1103 36-37 0.7624588046535479 1103 38-39 0.7315985090466413 1103 40-41 0.7833724520057288 1103 42-43 0.8063944998795023 1103 44-45 0.8808805950393221 1103 46-47 0.7292306561820183 1103 48-49 0.7014019624355328 1103 50-51 0.6690553202800302 1103 52-53 0.5955151249544599 1103 54-55 0.6421579764842775 1103 56-57 0.716504684845745 1103 58-59 0.7385548170641414 1103 60-61 0.6701855448164977 1103 62-63 0.8041437281962374 1103 64-65 0.6631633244143131 1103 66-67 0.7041494770439911 1103 68-69 0.6203146046361923 1103 70-71 0.6440622084213246 1103 72-73 0.6690983963244364 1103 74-75 0.6199910021463992 1103 76-77 0.5419470319452984 1103 78-79 0.6487521930543423 1103 80-81 0.7180012127310533 1103 82-83 0.7454044551158248 1103 84-85 0.576177651973989 1103 86-87 0.6454374292013618 1103 88-89 0.6640431134554703 1103 90-91 0.6242622456885982 1103 92-93 0.6688688972464796 1103 94-95 0.6153421354710993 1103 96-97 0.620344638421173 1103 98-99 0.6158655198812681 1103 100 0.7522763630406573 1104 1 0.39600780214459874 1104 2 3.748793608866958 1104 3 2.169269498751433 1104 4 0.9230375755778795 1104 5 0.324137480458333 1104 6 0.36817567376998284 1104 7 0.35912782468820126 1104 8 0.3683307586402904 1104 9 0.4208959726760071 1104 10-11 0.4358672647396773 1104 12-13 0.3683414909871914 1104 14-15 0.5483432011837621 1104 16-17 0.5283475745065402 1104 18-19 0.5480834335381104 1104 20-21 0.620891840495581 1104 22-23 0.5166468679584071 1104 24-25 0.5329588618164465 1104 26-27 0.4658953392096805 1104 28-29 0.5914103287646739 1104 30-31 0.42074436039207086 1104 32-33 0.5730697233548696 1104 34-35 0.7354485631889318 1104 36-37 0.5700726589433032 1104 38-39 0.5287050555472277 1104 40-41 0.500854000927724 1104 42-43 0.5860388686686022 1104 44-45 0.6039916598747581 1104 46-47 0.6374033698948978 1104 48-49 0.4064849680725544 1104 50-51 0.4531744386962444 1104 52-53 0.4566781482192823 1104 54-55 0.41526347209669723 1104 56-57 0.4536078171704858 1104 58-59 0.5460651564477459 1104 60-61 0.5833218890816525 1104 62-63 0.5812722610207572 1104 64-65 0.5195974719841985 1104 66-67 0.4582317175181956 1104 68-69 0.4679151170047078 1104 70-71 0.48092731699695435 1104 72-73 0.4658023848566799 1104 74-75 0.4563160171590894 1104 76-77 0.48840004223141165 1104 78-79 0.41006800674204413 1104 80-81 0.41884301289763926 1104 82-83 0.4864351003708478 1104 84-85 0.4657008538413727 1104 86-87 0.5348137730790903 1104 88-89 0.4500897883871886 1104 90-91 0.49145358966318753 1104 92-93 0.49123041730537054 1104 94-95 0.4491608790543786 1104 96-97 0.49144049201355955 1104 98-99 0.5127163664031684 1104 100 0.39805537627119847 1105 1 0.4922695496090057 1105 2 3.8113487524609972 1105 3 2.2469081003409066 1105 4 0.9883263979803161 1105 5 0.3901213686274829 1105 6 0.4032499819251498 1105 7 0.3827013681696272 1105 8 0.30397290564374657 1105 9 0.3149303841873845 1105 10-11 0.36346566429303806 1105 12-13 0.3681762093224279 1105 14-15 0.4979649799508863 1105 16-17 0.4477818292657716 1105 18-19 0.49277921029632665 1105 20-21 0.4549551032308017 1105 22-23 0.4342469793788837 1105 24-25 0.45536070085083935 1105 26-27 0.4055063934573724 1105 28-29 0.4767393591679152 1105 30-31 0.33839627517807 1105 32-33 0.4815828922058074 1105 34-35 0.6398775570882478 1105 36-37 0.4667393792922141 1105 38-39 0.39008094935659443 1105 40-41 0.40534288035262733 1105 42-43 0.5517195416292111 1105 44-45 0.5597846897721936 1105 46-47 0.5376385577708263 1105 48-49 0.465913001656169 1105 50-51 0.38065766001263057 1105 52-53 0.310061859356324 1105 54-55 0.4398103641333577 1105 56-57 0.3952108405544692 1105 58-59 0.4178467269754762 1105 60-61 0.31738655351990985 1105 62-63 0.4285464556081493 1105 64-65 0.36110866890737015 1105 66-67 0.3287439117381723 1105 68-69 0.3695114778257391 1105 70-71 0.3729617666823728 1105 72-73 0.28412914509745946 1105 74-75 0.2097458353790813 1105 76-77 0.23951135415388336 1105 78-79 0.24626439309195547 1105 80-81 0.3089712060964587 1105 82-83 0.44040800395992363 1105 84-85 0.4727169441993233 1105 86-87 0.43584900533234006 1105 88-89 0.3240839322508293 1105 90-91 0.3058958729002441 1105 92-93 0.2970489462687276 1105 94-95 0.33128770395244445 1105 96-97 0.3425948690251559 1105 98-99 0.291753599214708 1105 100 0.2514245932023371 1106 1 0.5325680412134872 1106 2 3.9587598536947937 1106 3 2.2325828290197833 1106 4 1.04580840336601 1106 5 0.4126576972657432 1106 6 0.26777157959426034 1106 7 0.232261611316801 1106 8 0.3600165814679812 1106 9 0.344549395377868 1106 10-11 0.3174573874197577 1106 12-13 0.31118454758457403 1106 14-15 0.4090746842335946 1106 16-17 0.38402818282555273 1106 18-19 0.40631896962831604 1106 20-21 0.3734038181373194 1106 22-23 0.3517573889253356 1106 24-25 0.3773057778761668 1106 26-27 0.43380191360414244 1106 28-29 0.3396496759227148 1106 30-31 0.3674517187776303 1106 32-33 0.4697389710621849 1106 34-35 0.5919337302709877 1106 36-37 0.4230365203414195 1106 38-39 0.32327609869093976 1106 40-41 0.37549436920995305 1106 42-43 0.3697834545325662 1106 44-45 0.38573483019924737 1106 46-47 0.2805313491820485 1106 48-49 0.2335562531888229 1106 50-51 0.25743073603212707 1106 52-53 0.2920382033890263 1106 54-55 0.267446367056408 1106 56-57 0.27953941152768635 1106 58-59 0.32357138724118073 1106 60-61 0.39850808696681383 1106 62-63 0.3265525862318768 1106 64-65 0.2906929422578628 1106 66-67 0.23439106854778657 1106 68-69 0.20818812254587726 1106 70-71 0.17463724029165206 1106 72-73 0.11236153504904678 1106 74-75 0.06265932782766015 1106 76-77 0.04123384337835745 1106 78-79 0.20444637923460363 1106 80-81 0.21298001776128928 1106 82-83 0.25774138923768675 1106 84-85 0.22448990216329534 1106 86-87 0.38903316959952505 1106 88-89 0.2331399130030576 1106 90-91 0.26197437510611365 1106 92-93 0.3617766098671389 1106 94-95 0.2606839148421223 1106 96-97 0.2263423116472154 1106 98-99 0.2963278673589613 1106 100 0.3144877328419611 1107 1 0.3959165454089799 1107 2 3.625159451437387 1107 3 2.1145716153728635 1107 4 0.9083910366483607 1107 5 0.2768153475243409 1107 6 0.29272206428195346 1107 7 0.13961492840666523 1107 8 0.16656980448927783 1107 9 0.22092973432544483 1107 10-11 0.176498239883081 1107 12-13 0.16386523110940487 1107 14-15 0.13776443960117746 1107 16-17 0.11453454718968459 1107 18-19 0.19481657611255088 1107 20-21 0.19239550413595907 1107 22-23 0.14786385842491967 1107 24-25 0.19212865951396907 1107 26-27 0.16978895992930632 1107 28-29 0.11148296645203715 1107 30-31 0.14432469974678952 1107 32-33 0.17976388960926215 1107 34-35 0.38605543719446445 1107 36-37 0.08849732700946333 1107 38-39 0.18878959338034207 1107 40-41 0.19347641197747123 1107 42-43 0.10309067441544073 1107 44-45 0.22990188731462524 1107 46-47 0.14656013091590125 1107 48-49 -0.0037123871534774366 1107 50-51 -0.012790141656481069 1107 52-53 0.012763601962475946 1107 54-55 0.037154559021246314 1107 56-57 0.021982845583750077 1107 58-59 0.0078446293648895 1107 60-61 0.0902403530268856 1107 62-63 0.07873813167017829 1107 64-65 0.052773640939520305 1107 66-67 0.07587652490965979 1107 68-69 0.13024831440004903 1107 70-71 0.0325488789913706 1107 72-73 0.1513945173895408 1107 74-75 0.27869831936930467 1107 76-77 0.22547350891768758 1107 78-79 0.015909106368987125 1107 80-81 0.10788551914855304 1107 82-83 0.166790165955355 1107 84-85 0.13674906948805798 1107 86-87 0.19757901930514876 1107 88-89 0.15379939140367327 1107 90-91 0.1645911834269711 1107 92-93 0.2081244458389797 1107 94-95 0.18381788075248906 1107 96-97 0.1472727333859538 1107 98-99 0.11448952154630376 1107 100 0.20810899378179215 1108 1 0.38162171327664396 1108 2 3.627268976331962 1108 3 2.0733759277283283 1108 4 0.9768173018964674 1108 5 0.16721543014249818 1108 6 -0.026710614380554887 1108 7 0.026888130013276168 1108 8 0.004185922474732706 1108 9 -0.027779402035641 1108 10-11 0.018556854942218592 1108 12-13 0.024226064099750033 1108 14-15 -0.03491253271963757 1108 16-17 -0.12567264965617397 1108 18-19 -0.024578921378129337 1108 20-21 -0.022742741675273237 1108 22-23 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-0.1348384682919601 2211 80-81 0.04460083637066248 2211 82-83 -0.32265134984206867 2211 84-85 -0.06293963425713756 2211 86-87 -0.05133193562240379 2211 88-89 0.0260544852206408 2211 90-91 0.05040933901478439 2211 92-93 0.09426496246265614 2211 94-95 0.09090833192032832 2211 96-97 0.05611103666551287 2211 98-99 0.020912991775666967 2211 100 -0.1012315748223429 2212 1 0.6578950863490824 2212 2 4.1334850484111065 2212 3 2.3707769865293074 2212 4 1.0911758024220504 2212 5 0.6495597830373967 2212 6 0.40398053172108206 2212 7 0.30775975561892466 2212 8 0.40602714957181973 2212 9 0.29558126896834835 2212 10-11 0.3355588146416366 2212 12-13 0.26593118430282914 2212 14-15 0.3160678621093709 2212 16-17 0.32413728584727863 2212 18-19 0.32306816737344235 2212 20-21 0.34115799596031593 2212 22-23 0.27499493099479366 2212 24-25 0.28321010977942507 2212 26-27 0.08162390312341472 2212 28-29 0.1803182186128609 2212 30-31 0.1920110474136223 2212 32-33 0.23781132052676668 2212 34-35 0.35257191196009785 2212 36-37 0.5166162061378259 2212 38-39 0.2541768811965923 2212 40-41 0.3329988878661325 2212 42-43 0.4166507016015828 2212 44-45 0.33725460944491914 2212 46-47 0.5619803315970557 2212 48-49 0.296716645916014 2212 50-51 0.37074202366256515 2212 52-53 0.2625128579598339 2212 54-55 0.31558934953330464 2212 56-57 0.2826815840138437 2212 58-59 0.1507549038387097 2212 60-61 0.13722423344954393 2212 62-63 0.34091413151217864 2212 64-65 0.44549950918949577 2212 66-67 0.3643880743123802 2212 68-69 0.288302849997649 2212 70-71 0.34714265084774354 2212 72-73 0.393697346286622 2212 74-75 0.3965133001496426 2212 76-77 0.32865678049576985 2212 78-79 0.2534950951717647 2212 80-81 0.1670693788515223 2212 82-83 0.4740364916150597 2212 84-85 0.47187417814988564 2212 86-87 0.3642570546791397 2212 88-89 0.4955044670804014 2212 90-91 0.26604043758643314 2212 92-93 0.48528217696076226 2212 94-95 0.43293768220914686 2212 96-97 0.3483974947688253 2212 98-99 0.44247945575988723 2212 100 0.21214549446441922 2213 1 -0.5586863173314782 2213 2 -25.77445813938479 2213 3 -14.649538371445919 2213 4 -6.089137252586685 2213 5 -2.177893376670859 2213 6 -1.4025747814182665 2213 7 -1.2620537513245011 2213 8 -1.151527109015781 2213 9 -1.1946622719865587 2213 10-11 -1.1208760987800943 2213 12-13 -1.1422451113800776 2213 14-15 -1.3669202548984174 2213 16-17 -1.4001971759546237 2213 18-19 -1.3691520564955866 2213 20-21 -1.2322649161722907 2213 22-23 -1.2992047825287685 2213 24-25 -1.334612152035028 2213 26-27 -1.3641602400977177 2213 28-29 -1.466231863374091 2213 30-31 -1.3994359509596777 2213 32-33 -1.2223402749409757 2213 34-35 -1.1951899037310696 2213 36-37 -1.326299102044743 2213 38-39 -1.444615793120981 2213 40-41 -1.447186559409765 2213 42-43 -1.445700271840309 2213 44-45 -1.2564169780883887 2213 46-47 -1.3519883521069147 2213 48-49 -1.458830883241589 2213 50-51 -1.361902009011665 2213 52-53 -1.4555176828841994 2213 54-55 -1.372498578806649 2213 56-57 -1.4558505312361305 2213 58-59 -1.5280421065329648 2213 60-61 -1.4276294618333623 2213 62-63 -1.3941500400642184 2213 64-65 -1.3504144795102277 2213 66-67 -1.4555045408792395 2213 68-69 -1.4247371804626852 2213 70-71 -1.4065793725511995 2213 72-73 -1.397538741737975 2213 74-75 -1.437029564151434 2213 76-77 -1.4371461462957527 2213 78-79 -1.5137002942173652 2213 80-81 -1.604587895655115 2213 82-83 -1.4528433104017964 2213 84-85 -1.347176190021134 2213 86-87 -1.3069327446186811 2213 88-89 -1.2929199327595597 2213 90-91 -1.3688313103448877 2213 92-93 -1.276205049109361 2213 94-95 -1.5824925305971043 2213 96-97 -1.5239410215135294 2213 98-99 -1.348299749802134 2213 100 -1.5378436541554095 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 7.0 4 25.0 5 44.0 6 43.0 7 41.0 8 54.0 9 66.0 10 376.0 11 1724.0 12 5292.0 13 9671.0 14 12787.0 15 14612.0 16 15849.0 17 16433.0 18 16679.0 19 17741.0 20 19373.0 21 22218.0 22 24412.0 23 25878.0 24 26343.0 25 27801.0 26 30500.0 27 34496.0 28 40337.0 29 48106.0 30 58007.0 31 70802.0 32 89193.0 33 118242.0 34 161465.0 35 228318.0 36 346275.0 37 592710.0 38 624744.0 39 3970.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 38.102789572487175 20.98110780118002 10.553445904118671 30.362656722214126 2 12.678126104553478 22.653109500699657 36.944584907577436 27.724179487169426 3 15.081295627690983 27.133605828512902 29.052977573683698 28.73212097011242 4 10.319830859177989 18.858144154244126 38.0315046226796 32.79052036389828 5 11.305610179722768 38.830746554974134 35.502931188362645 14.360712076940455 6 29.211568982164852 40.23014402036188 17.85637918086338 12.70190781660988 7 25.574464040076833 33.47343012501447 23.374847009938946 17.577258824969753 8 23.99493537536 37.34687204313882 20.95889782641343 17.699294755087745 9 24.27445005568705 17.15704932234166 22.252553263342605 36.315947358628684 10-11 22.58296298858181 28.058578493883463 29.486716877825902 19.871741639708823 12-13 23.479811674139373 25.46286663740836 29.72357555773421 21.333746130718062 14-15 20.963692397151824 27.232745847889774 26.834545719866487 24.96901603509192 16-17 20.242428389401873 30.677038930200816 27.691983348651934 21.388549331745377 18-19 20.220075078129334 29.605673391645247 29.81733454148714 20.35691698873828 20-21 21.443728839258803 28.929107009787337 29.08382196038839 20.543342190565475 22-23 20.757466912597483 28.80338016188905 29.303359359470416 21.135793566043056 24-25 20.859200741690056 28.75093677384426 29.152734861891844 21.237127622573844 26-27 20.694096386745077 28.724901480719726 29.205618621946662 21.375383510588534 28-29 20.49216626638041 28.717008742074324 29.50482904427026 21.285995947275005 30-31 21.047574632730523 29.091259757516895 28.93840878924503 20.922756820507548 32-33 20.30738314837102 28.920766618415627 29.623974665431735 21.147875567781618 34-35 20.446625296860955 28.565631460180608 29.664779599112457 21.32296364384598 36-37 20.924724988473013 28.979287659968133 29.030461194081177 21.065526157477677 38-39 20.72508503517696 28.990016154742392 29.24361492034111 21.04128388973954 40-41 20.80427796571815 28.78747093230708 29.188252899004354 21.219998202970423 42-43 20.611313215797704 28.781697912316208 29.297605637213987 21.309383234672104 44-45 20.91453772543457 28.609897406666985 29.171072270514646 21.3044925973838 46-47 20.92454717190799 29.01082187168513 29.000190259631363 21.064440696775517 48-49 20.884971134607774 28.891132536715077 28.991449482789868 21.23244684588728 50-51 21.054655862959088 28.770270820004363 28.765851457649934 21.409221859386616 52-53 20.781686700290066 28.92920927070855 28.768865082690702 21.520238946310677 54-55 21.07935448940224 28.97144123007373 28.549191410549234 21.400012869974795 56-57 21.12147013257012 28.695452966256273 28.644014301501947 21.539062599671656 58-59 20.700377795540003 28.4837091967227 29.168467079758624 21.64744592797867 60-61 21.169462604238678 28.83888517978982 28.58989380102618 21.401758414945327 62-63 20.709314643017546 28.94781364390341 28.8426972587089 21.500174454370153 64-65 20.691243421629235 28.469151042092328 28.977133840237535 21.862471696040902 66-67 20.827231469935196 28.737003030961965 28.71394538351506 21.721820115587782 68-69 21.101101500923757 28.69967711471738 28.689931937239578 21.509289447119283 70-71 21.110498211197257 28.589241442973094 28.56326048618229 21.73699985964736 72-73 20.854164451573833 28.733842149788046 28.868901945737548 21.54309145290058 74-75 21.129020924710982 28.405905369122554 28.640799857987115 21.824273848179352 76-77 20.937799308470314 28.767487010705768 28.65294085017473 21.641772830649188 78-79 21.018046610008884 28.69439650032033 28.758092593226024 21.529464296444758 80-81 21.07735564077471 28.507587723782706 28.61216309986856 21.802893535574025 82-83 20.688007794697718 28.71356981810215 28.8377007490564 21.760721638143732 84-85 20.790454757571343 28.423180204278896 28.68501838894637 22.101346649203393 86-87 20.88191079686607 28.644000768377314 28.584246305234746 21.889842129521867 88-89 20.72778535277669 28.33807399875205 28.846349749254696 22.087790899216568 90-91 20.87493402035362 28.337849068150156 28.719629718989104 22.067587192507116 92-93 20.576158619621207 28.70565824118249 28.745923972826365 21.97225916636994 94-95 20.501542003084374 28.271640269038034 28.9270424192274 22.29977530865019 96-97 20.643197500566163 28.43324136082341 28.76486003078075 22.15870110782968 98-99 20.613265449490132 28.327436016889617 28.804305225872767 22.254993307747483 100 20.72552227535867 28.237514990894418 28.632719385262284 22.404243348484624 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 28.0 1 23.0 2 18.5 3 21.0 4 22.0 5 32.0 6 63.5 7 101.5 8 156.0 9 204.5 10 195.5 11 194.5 12 222.0 13 269.0 14 341.5 15 415.0 16 525.0 17 682.5 18 854.5 19 1100.0 20 1508.0 21 2135.5 22 3114.0 23 4502.5 24 6692.0 25 9992.0 26 14604.0 27 20917.0 28 28959.5 29 38429.0 30 48812.0 31 59322.0 32 69534.5 33 79535.5 34 88360.5 35 96154.0 36 102808.5 37 107932.5 38 112420.0 39 115332.5 40 116341.5 41 115134.0 42 112182.0 43 108597.5 44 104146.0 45 99042.0 46 94066.0 47 89894.0 48 85661.5 49 82410.0 50 83806.5 51 78759.5 52 67108.0 53 59903.5 54 55098.0 55 51248.5 56 47128.5 57 42335.5 58 37294.5 59 32382.0 60 27415.5 61 22612.5 62 18122.5 63 14188.0 64 10932.0 65 8458.5 66 6490.0 67 4814.0 68 3554.5 69 2567.0 70 1863.5 71 1381.5 72 1012.0 73 740.5 74 518.0 75 338.0 76 227.5 77 146.0 78 78.5 79 43.5 80 31.0 81 17.0 82 8.0 83 6.0 84 4.5 85 1.5 86 0.5 87 0.5 88 0.5 89 0.0 90 1.0 91 1.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content warn #Base N-Count 1 0.793600615240134 2 5.750424733836543 3 0.0 4 0.005287219902130971 5 0.1613896144951167 6 0.057124159082463995 7 0.038008825590144325 8 0.0435178868867703 9 0.0419280235595561 10-11 0.04383216219563823 12-13 0.04176164251368484 14-15 0.045126236996859095 16-17 0.035124887461709255 18-19 0.029024249113096593 20-21 0.02329334642197561 22-23 0.023016044678856852 24-25 0.019984212287425106 26-27 0.01264495948621533 28-29 0.019540529498435093 30-31 0.025659654629922337 32-33 0.0011092069724750288 34-35 0.0031797266544284163 36-37 0.004141039363906775 38-39 0.006137611914361827 40-41 0.006359453308856833 42-43 0.006599781486226423 44-45 0.008115697681942294 46-47 0.016101987883762506 48-49 0.02606636385316318 50-51 0.02310847859322977 52-53 0.02824780423236407 54-55 0.02466136835469481 56-57 0.016397776409755843 58-59 0.014586071688046631 60-61 0.011997922085604898 62-63 0.018098560434217554 64-65 0.01878257140057716 66-67 0.020076646201798026 68-69 0.02712011047701446 70-71 0.02704616334551612 72-73 0.023940383822586044 74-75 0.024217685565704798 76-77 0.017932179388346303 78-79 0.014327256727802459 80-81 0.01351383828132077 82-83 0.008596354036681474 84-85 0.010833254764506117 86-87 0.008670301168179809 88-89 0.007302279235460608 90-91 0.007487147064206446 92-93 0.0037713037064150984 94-95 0.0013310483669700347 96-97 8.319052293562717E-4 98-99 5.546034862375144E-4 100 0.11073582941875706 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 2704635.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 88.49469270269522 #Duplication Level Percentage of deduplicated Percentage of total 1 91.67755572823701 81.1297712190455 2 6.669590554205514 11.80446733094431 3 1.0672287064205839 2.833322292545535 4 0.28156168984061164 0.9966686087718604 5 0.10851635923692106 0.48015609319433056 6 0.05287310477443732 0.28073934955507424 7 0.03373344894233881 0.20896618385680324 8 0.02373262341038171 0.1680168972584416 9 0.016612925827127194 0.13231401893678607 >10 0.06451275408018498 0.941962534943374 >50 0.0018150312997122328 0.11179746649225689 >100 0.0017230131364765514 0.31628314934458635 >500 3.3480651611019163E-4 0.22843650413088928 >1k 2.0925407255645214E-4 0.36709835098022436 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 3487 0.12892682376734754 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 3.6973565749167633E-5 0.0 9 7.394713149833527E-5 0.0 0.0 3.6973565749167633E-5 0.0 10-11 7.394713149833527E-5 0.0 0.0 3.6973565749167633E-5 0.0 12-13 7.394713149833527E-5 1.8486782874583817E-5 0.0 3.6973565749167633E-5 0.0 14-15 7.394713149833527E-5 5.546034862375145E-5 0.0 3.6973565749167633E-5 0.0 16-17 7.394713149833527E-5 7.394713149833527E-5 0.0 3.6973565749167633E-5 0.0 18-19 7.394713149833527E-5 7.394713149833527E-5 0.0 3.6973565749167633E-5 0.0 20-21 7.394713149833527E-5 7.394713149833527E-5 0.0 7.394713149833527E-5 0.0 22-23 7.394713149833527E-5 7.394713149833527E-5 0.0 1.8486782874583814E-4 0.0 24-25 7.394713149833527E-5 7.394713149833527E-5 0.0 2.9578852599334107E-4 0.0 26-27 7.394713149833527E-5 9.243391437291908E-5 0.0 4.806563547391792E-4 0.0 28-29 1.109206972475029E-4 1.109206972475029E-4 0.0 7.949316636071041E-4 0.0 30-31 1.4789426299667053E-4 1.2940748012208673E-4 0.0 0.0024402553394450638 0.0 32-33 1.4789426299667053E-4 1.4789426299667053E-4 0.0 0.006525834354728087 0.0 34-35 1.4789426299667053E-4 1.4789426299667053E-4 0.0 0.014123902116182037 0.0 36-37 1.4789426299667053E-4 1.4789426299667053E-4 0.0 0.026528533425027777 0.0 38-39 1.4789426299667053E-4 1.4789426299667053E-4 0.0 0.0461430100549612 0.0 40-41 1.4789426299667053E-4 1.4789426299667053E-4 0.0 0.06915905473381806 0.0 42-43 1.4789426299667053E-4 1.8486782874583817E-4 0.0 0.09160200914356281 0.0 44-45 1.4789426299667053E-4 1.8486782874583817E-4 3.6973565749167633E-5 0.12060777147378482 0.0 46-47 1.4789426299667053E-4 2.218413944950058E-4 3.6973565749167633E-5 0.15591752676423992 0.0 48-49 1.8486782874583817E-4 2.218413944950058E-4 3.6973565749167633E-5 0.19547924211584927 0.0 50-51 1.8486782874583817E-4 2.218413944950058E-4 3.6973565749167633E-5 0.2374996995897783 0.0 52-53 2.218413944950058E-4 2.588149602441734E-4 3.6973565749167633E-5 0.2803520622930636 0.0 54-55 2.218413944950058E-4 2.588149602441734E-4 3.6973565749167633E-5 0.33087643988930115 0.0 56-57 2.218413944950058E-4 2.588149602441734E-4 3.6973565749167633E-5 0.39125427275769187 0.0 58-59 2.218413944950058E-4 2.588149602441734E-4 3.6973565749167633E-5 0.46078306314900164 0.0 60-61 2.218413944950058E-4 2.588149602441734E-4 3.6973565749167633E-5 0.5325117807023868 0.0 62-63 2.218413944950058E-4 2.588149602441734E-4 3.6973565749167633E-5 0.6069950289040851 0.0 64-65 2.218413944950058E-4 2.588149602441734E-4 3.6973565749167633E-5 0.6918308755155502 0.0 66-67 2.218413944950058E-4 2.588149602441734E-4 3.6973565749167633E-5 0.7931754192340186 0.0 68-69 2.218413944950058E-4 2.588149602441734E-4 3.6973565749167633E-5 0.9034860526466603 0.0 70-71 2.218413944950058E-4 2.7730174311875726E-4 3.6973565749167633E-5 1.0282903238329757 0.0 72-73 2.218413944950058E-4 2.9578852599334107E-4 3.6973565749167633E-5 1.1581784603098015 0.0 74-75 2.218413944950058E-4 2.9578852599334107E-4 3.6973565749167633E-5 1.2990292590312555 0.0 76-77 2.218413944950058E-4 3.327620917425087E-4 3.6973565749167633E-5 1.4579231578383034 0.0 78-79 2.218413944950058E-4 3.512488746170925E-4 3.6973565749167633E-5 1.6362096918807898 0.0 80-81 2.218413944950058E-4 4.0670922324084394E-4 3.6973565749167633E-5 1.8341661629018333 0.0 82-83 2.218413944950058E-4 4.0670922324084394E-4 3.6973565749167633E-5 2.0502951414885926 0.0 84-85 2.218413944950058E-4 4.0670922324084394E-4 3.6973565749167633E-5 2.272654165904087 0.0 86-87 2.403281773695896E-4 4.0670922324084394E-4 3.6973565749167633E-5 2.5137772749372838 0.0 88 2.588149602441734E-4 4.0670922324084394E-4 3.6973565749167633E-5 2.71404459381765 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 7520 0.0 21.309605 1 ATCAACG 8365 0.0 19.166851 3 CAACGCA 8350 0.0 19.144974 5 TCAACGC 8355 0.0 19.133516 4 AACGCAG 8500 0.0 18.78207 6 TATCAAC 8815 0.0 18.33559 2 GAGTACT 3370 0.0 17.41274 12-13 CCTACAC 1120 0.0 17.211874 3 ACGCAGA 9360 0.0 16.952898 7 CGCAGAG 9415 0.0 16.853865 8 GTACATG 9690 0.0 16.64052 1 CATGGGG 3650 0.0 16.61725 4 TAGGGCG 170 0.0019146868 16.594519 5 TACATGG 9910 0.0 15.8565645 2 TACGACC 180 0.0028043005 15.672601 4 ACATGGG 9740 0.0 15.302509 3 GCGTCAG 300 1.6067284E-5 14.976469 1 GCCTTAC 710 0.0 14.765533 1 GCAGAGT 10535 0.0 14.705592 9 TATACTG 1640 0.0 14.334696 5 >>END_MODULE