##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1295083_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 247003 Sequences flagged as poor quality 0 Sequence length 100 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 25.833499997975732 27.0 22.0 33.0 6.0 33.0 2 21.793180649627736 27.0 2.0 33.0 2.0 33.0 3 24.394185495722724 27.0 15.0 33.0 15.0 33.0 4 24.175034311324154 33.0 6.0 37.0 2.0 37.0 5 26.416529353894486 33.0 15.0 37.0 2.0 37.0 6 27.047274729456728 33.0 15.0 37.0 2.0 37.0 7 27.043218098565603 33.0 15.0 37.0 6.0 37.0 8 27.777865046173527 33.0 15.0 37.0 6.0 37.0 9 28.496973720966952 33.0 22.0 37.0 6.0 37.0 10-11 28.18192086735789 33.0 18.5 37.0 6.0 37.0 12-13 27.035724262458352 33.0 15.0 37.0 4.0 37.0 14-15 28.777567478937506 33.0 22.0 37.0 6.0 40.0 16-17 29.12202078517265 33.0 22.0 37.0 6.0 40.0 18-19 29.280279996599234 33.0 22.0 37.0 6.0 40.0 20-21 28.781800221049945 33.0 22.0 37.0 6.0 40.0 22-23 29.65614587677073 33.0 27.0 37.0 6.0 40.0 24-25 29.902855430905696 33.0 27.0 37.0 10.5 40.0 26-27 28.233515382404264 33.0 18.5 37.0 4.0 40.0 28-29 28.95189532110946 33.0 22.0 37.0 6.0 40.0 30-31 29.63435464346587 33.0 27.0 37.0 10.5 40.0 32-33 28.1929430006923 33.0 22.0 37.0 6.0 40.0 34-35 27.938940012874337 33.0 22.0 37.0 6.0 40.0 36-37 28.27714643141986 33.0 22.0 37.0 6.0 40.0 38-39 28.289953968170426 33.0 22.0 37.0 6.0 40.0 40-41 27.308633903231943 33.0 15.0 37.0 6.0 40.0 42-43 27.719655226859594 33.0 15.0 37.0 6.0 40.0 44-45 27.520323639793848 33.0 22.0 37.0 6.0 40.0 46-47 27.052849560531655 33.0 15.0 37.0 6.0 40.0 48-49 26.92102929923928 33.0 15.0 37.0 6.0 40.0 50-51 26.9707513673923 33.0 18.5 37.0 6.0 40.0 52-53 27.358872159447458 33.0 18.5 37.0 6.0 40.0 54-55 27.224047886058063 33.0 22.0 37.0 6.0 40.0 56-57 27.05914907916098 33.0 22.0 37.0 6.0 40.0 58-59 26.857799297984236 33.0 22.0 37.0 6.0 40.0 60-61 26.718309089363288 30.0 18.5 37.0 6.0 40.0 62-63 26.399300818208687 27.0 18.5 37.0 6.0 37.0 64-65 26.125470135990252 27.0 15.0 37.0 6.0 37.0 66-67 26.359321546701864 27.0 18.5 37.0 6.0 37.0 68-69 25.81856293243402 27.0 18.5 35.0 6.0 37.0 70-71 25.69729719881945 27.0 15.0 33.0 6.0 37.0 72-73 25.475666287453997 27.0 15.0 33.0 6.0 37.0 74-75 25.532827536507654 27.0 22.0 33.0 6.0 37.0 76-77 25.127619907450516 27.0 15.0 33.0 6.0 37.0 78-79 24.707859823564895 27.0 15.0 33.0 6.0 37.0 80-81 24.672066736031546 27.0 15.0 33.0 6.0 37.0 82-83 24.91632287866949 27.0 15.0 33.0 6.0 37.0 84-85 24.705993449472274 27.0 15.0 33.0 6.0 37.0 86-87 24.244575976809998 27.0 15.0 33.0 6.0 37.0 88-89 24.161064845366248 27.0 15.0 33.0 6.0 37.0 90-91 24.3496880604689 27.0 15.0 33.0 6.0 37.0 92-93 24.02670615336656 27.0 15.0 33.0 6.0 37.0 94-95 24.061209782877132 27.0 15.0 33.0 6.0 33.0 96-97 24.281326542592602 27.0 15.0 33.0 6.0 37.0 98-99 24.274035133176522 27.0 15.0 33.0 6.0 37.0 100 23.652579118472246 27.0 15.0 33.0 6.0 33.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 6.9141444007217565 1101 2 10.771569840675262 1101 3 8.049610803664482 1101 4 12.180582158993726 1101 5 10.503430351547188 1101 6 9.35492908989104 1101 7 8.915945556693163 1101 8 7.824714528278225 1101 9 6.521138140274889 1101 10-11 6.933722367052987 1101 12-13 8.273655364458541 1101 14-15 7.5006033483344865 1101 16-17 7.509811637694845 1101 18-19 7.381619200236852 1101 20-21 6.856661595318794 1101 22-23 6.75966637077892 1101 24-25 7.048672102962936 1101 26-27 7.641264684828947 1101 28-29 7.4772308001228325 1101 30-31 4.71298028953769 1101 32-33 6.344234835623055 1101 34-35 7.7285904690604745 1101 36-37 7.556771122463864 1101 38-39 7.388815618460519 1101 40-41 7.865300544220656 1101 42-43 7.811697477152482 1101 44-45 7.930326500050928 1101 46-47 7.065929214548415 1101 48-49 4.967632318851294 1101 50-51 6.167551328158666 1101 52-53 5.2139017810696195 1101 54-55 3.340003389315193 1101 56-57 5.749798957759246 1101 58-59 5.807793691808609 1101 60-61 5.911986740516927 1101 62-63 5.320437479346914 1101 64-65 5.943678698407179 1101 66-67 4.828720152076851 1101 68-69 6.1500917559097985 1101 70-71 5.667194994892775 1101 72-73 4.558295658511657 1101 74-75 4.507546293778542 1101 76-77 4.248678861276346 1101 78-79 4.844922671575958 1101 80-81 4.800442690529756 1101 82-83 4.277033177937341 1101 84-85 4.034017646631533 1101 86-87 3.367635675561022 1101 88-89 3.315593222856883 1101 90-91 3.265746079833253 1101 92-93 2.9336274543754897 1101 94-95 3.159422899441399 1101 96-97 3.3085335934102105 1101 98-99 3.2112213816273716 1101 100 3.124625868422001 1102 1 6.612999276958686 1102 2 10.472635772892907 1102 3 7.940294934519752 1102 4 12.50972464771068 1102 5 10.895442517611158 1102 6 9.56747779619553 1102 7 7.108924139935393 1102 8 7.828078416933511 1102 9 6.315313707230292 1102 10-11 7.384206626857328 1102 12-13 9.081610610723224 1102 14-15 6.158243580442804 1102 16-17 7.036600105163238 1102 18-19 7.1924845605934244 1102 20-21 7.028649162946973 1102 22-23 6.969068439570439 1102 24-25 7.206871231155123 1102 26-27 8.749084324332209 1102 28-29 8.058270732284413 1102 30-31 3.921166452742355 1102 32-33 4.641797067563179 1102 34-35 4.887313452829712 1102 36-37 4.978164753501339 1102 38-39 4.548486447939396 1102 40-41 6.074797222380607 1102 42-43 7.102747430899008 1102 44-45 6.166490755930727 1102 46-47 5.0712658837380005 1102 48-49 3.5228123569753684 1102 50-51 4.808195162431087 1102 52-53 6.180313703251663 1102 54-55 5.863864222438288 1102 56-57 6.544549189026593 1102 58-59 6.155344710786547 1102 60-61 5.80290914681861 1102 62-63 4.67106680684951 1102 64-65 5.805718966957624 1102 66-67 3.5050001817911998 1102 68-69 5.8610285989002975 1102 70-71 5.336014900703891 1102 72-73 5.295003042247256 1102 74-75 4.418110656181479 1102 76-77 3.856354834701623 1102 78-79 4.385096612651839 1102 80-81 4.697125756153618 1102 82-83 3.4982106090476996 1102 84-85 3.2418866932494232 1102 86-87 3.51237749710673 1102 88-89 3.622844154934363 1102 90-91 3.5436751157988304 1102 92-93 3.4938988781413585 1102 94-95 3.20092837977667 1102 96-97 3.6771404529398275 1102 98-99 3.3119558306504437 1102 100 3.606555479051888 1103 1 6.8261874463631145 1103 2 11.014918315691489 1103 3 8.06915979782412 1103 4 12.153218974914122 1103 5 10.19083571602912 1103 6 8.980686174035537 1103 7 8.239896454378648 1103 8 8.2643641064742 1103 9 7.30069146062964 1103 10-11 7.391393251759887 1103 12-13 8.404833711852014 1103 14-15 8.347618273707617 1103 16-17 7.9439317977640584 1103 18-19 7.2201574870635845 1103 20-21 8.292971891663154 1103 22-23 7.27803918064264 1103 24-25 5.699318328357702 1103 26-27 5.753705199646163 1103 28-29 7.074664825647389 1103 30-31 6.233002461287633 1103 32-33 8.075334770730134 1103 34-35 7.248950624803502 1103 36-37 4.49527155508337 1103 38-39 6.786379429838412 1103 40-41 6.846481595486065 1103 42-43 7.883769183836453 1103 44-45 5.829769502430338 1103 46-47 6.491843123265699 1103 48-49 5.487032600053979 1103 50-51 6.23436400391034 1103 52-53 6.417854841852662 1103 54-55 4.820887014666699 1103 56-57 5.933283707921479 1103 58-59 6.006271411903786 1103 60-61 5.558293251440567 1103 62-63 3.3116904536060545 1103 64-65 5.404675345605966 1103 66-67 4.346214203558574 1103 68-69 5.38188117836275 1103 70-71 4.212822833958317 1103 72-73 4.198383263962665 1103 74-75 3.34313736018564 1103 76-77 3.5982895037163196 1103 78-79 2.932866106574874 1103 80-81 4.32418268620356 1103 82-83 3.4013977680303498 1103 84-85 2.9388413541807417 1103 86-87 2.6253011525240346 1103 88-89 2.966077215934966 1103 90-91 3.1091378168005903 1103 92-93 2.8471170715072205 1103 94-95 3.1345472775508547 1103 96-97 3.2893766606825423 1103 98-99 3.0284801962499124 1103 100 3.408289074132579 1104 1 6.28774609797199 1104 2 9.664548018886538 1104 3 7.631173871940231 1104 4 11.955613844671799 1104 5 9.18281261858857 1104 6 7.804851942153125 1104 7 3.8195315488681807 1104 8 6.8407502366597726 1104 9 6.627347155539123 1104 10-11 6.75989887005624 1104 12-13 7.189898407385751 1104 14-15 7.116572930083535 1104 16-17 7.159870876136459 1104 18-19 6.430676895723703 1104 20-21 6.871564196299669 1104 22-23 6.213212411446801 1104 24-25 5.865973172010154 1104 26-27 8.04073842704529 1104 28-29 6.914593036305121 1104 30-31 6.3025749883859845 1104 32-33 7.696018188243325 1104 34-35 7.8471431223916035 1104 36-37 6.225965755185527 1104 38-39 6.993312918289689 1104 40-41 7.02757943329323 1104 42-43 7.181159371680501 1104 44-45 6.435868049618353 1104 46-47 6.564448253508022 1104 48-49 5.479676320614672 1104 50-51 5.4136422798311195 1104 52-53 5.579192876016442 1104 54-55 5.034584449545537 1104 56-57 6.117318382457544 1104 58-59 3.1860001446658295 1104 60-61 3.362192008605316 1104 62-63 2.972184324555492 1104 64-65 2.9707045153466183 1104 66-67 3.234589579530425 1104 68-69 3.2239056094393064 1104 70-71 2.2720986979841946 1104 72-73 2.1101327390208304 1104 74-75 2.8502027934754643 1104 76-77 3.4055436323128774 1104 78-79 3.730447827249069 1104 80-81 3.0003999837462665 1104 82-83 2.8697310067795705 1104 84-85 3.0539348505770896 1104 86-87 0.8096302201138421 1104 88-89 0.6259716600641312 1104 90-91 2.0223415226061654 1104 92-93 2.115114063732406 1104 94-95 2.0920908335351918 1104 96-97 3.2481758207756144 1104 98-99 2.2561737153464456 1104 100 0.5009484010720264 1105 1 6.094670773223239 1105 2 -2.7388754478329 1105 3 0.5492269342608971 1105 4 8.274758119340689 1105 5 8.19537852244315 1105 6 8.316555618634105 1105 7 5.156294945195942 1105 8 6.744223175441629 1105 9 4.571761759691949 1105 10-11 5.35929884461347 1105 12-13 5.371397269528316 1105 14-15 6.02848438922668 1105 16-17 6.161372706602769 1105 18-19 6.217282688195471 1105 20-21 7.441358675217504 1105 22-23 2.239785976403617 1105 24-25 4.045355336624912 1105 26-27 7.057244427299715 1105 28-29 5.915444662522489 1105 30-31 5.533524710635696 1105 32-33 7.154896303216393 1105 34-35 6.246107036055786 1105 36-37 6.384548451271577 1105 38-39 4.401863810907045 1105 40-41 4.9209212004352985 1105 42-43 5.6267330355814416 1105 44-45 5.815714544876105 1105 46-47 4.9711404100659 1105 48-49 4.753712675513114 1105 50-51 6.152839701629922 1105 52-53 5.680730750413947 1105 54-55 5.244342460485932 1105 56-57 5.173691797584688 1105 58-59 2.0685251987317272 1105 60-61 4.787902639444784 1105 62-63 -0.7748968237617504 1105 64-65 -0.3569348191834685 1105 66-67 1.3314240742793224 1105 68-69 2.6715076278994516 1105 70-71 2.8822729809926635 1105 72-73 2.5544872699550076 1105 74-75 2.717034602598112 1105 76-77 2.001406354407102 1105 78-79 3.140104773833375 1105 80-81 1.9122160324833146 1105 82-83 1.1369578856578286 1105 84-85 1.6891985366697853 1105 86-87 0.1362836471142792 1105 88-89 1.9970792688673313 1105 90-91 1.2047344155910231 1105 92-93 1.8199920090869774 1105 94-95 1.5062320692059608 1105 96-97 2.6883252264041246 1105 98-99 2.232098489541819 1105 100 2.2689809678239747 1106 1 6.4910067997805285 1106 2 10.449192975602266 1106 3 7.118658613707801 1106 4 11.278983143003565 1106 5 10.340894921845063 1106 6 9.170895184334594 1106 7 7.527843251757073 1106 8 7.9885200793772135 1106 9 7.17063142868604 1106 10-11 6.161134260737143 1106 12-13 8.527589398364107 1106 14-15 8.450787850179502 1106 16-17 8.120960629619546 1106 18-19 7.460674229917583 1106 20-21 8.64997607737666 1106 22-23 6.622613587207606 1106 24-25 5.531428533788112 1106 26-27 8.217565556533316 1106 28-29 6.429865299293908 1106 30-31 5.866845469493839 1106 32-33 7.460781843484437 1106 34-35 6.750630417720831 1106 36-37 6.295627478418723 1106 38-39 7.604445464976372 1106 40-41 7.283096950445586 1106 42-43 6.652484417630578 1106 44-45 4.819281123292814 1106 46-47 6.508606630453009 1106 48-49 5.6077367361998185 1106 50-51 6.85967724398304 1106 52-53 6.096418894284231 1106 54-55 5.328187917899612 1106 56-57 4.957303369759892 1106 58-59 5.5671157143766585 1106 60-61 5.307535967748326 1106 62-63 5.101816162698967 1106 64-65 5.384862050458089 1106 66-67 3.124126992014819 1106 68-69 3.5976691664665097 1106 70-71 2.9434363714369773 1106 72-73 3.010656078854989 1106 74-75 2.7388387425725966 1106 76-77 3.3171651439652585 1106 78-79 3.9623554219904165 1106 80-81 3.3474037579665143 1106 82-83 1.8519402585298828 1106 84-85 2.7009751042495864 1106 86-87 3.3443523449016332 1106 88-89 2.9882862813092004 1106 90-91 2.972224367442216 1106 92-93 2.8124679003886435 1106 94-95 0.9959850150625584 1106 96-97 1.4995297696763217 1106 98-99 1.7058601323627052 1106 100 3.3347774808076913 1107 1 6.012951907914356 1107 2 10.04959073282659 1107 3 6.2035089241775125 1107 4 11.106061441615864 1107 5 9.480568637691682 1107 6 7.839770852683117 1107 7 3.9273428033388598 1107 8 6.550535291366067 1107 9 5.541421945496584 1107 10-11 4.385548749779993 1107 12-13 6.07462367668462 1107 14-15 5.872588506033928 1107 16-17 6.602048334745199 1107 18-19 6.1796751815226365 1107 20-21 5.064768059248237 1107 22-23 5.681340969472149 1107 24-25 4.47807874704349 1107 26-27 6.720228681494806 1107 28-29 5.904459497926066 1107 30-31 4.33832983647109 1107 32-33 4.4105966570314585 1107 34-35 5.9163072553797456 1107 36-37 5.8512946631054135 1107 38-39 5.500564764759353 1107 40-41 6.918045159853467 1107 42-43 5.45533101752515 1107 44-45 3.2433272932535893 1107 46-47 2.2711435137068143 1107 48-49 3.4011528614556923 1107 50-51 4.134567877861553 1107 52-53 3.537318400681894 1107 54-55 4.533741457665066 1107 56-57 4.274210105617598 1107 58-59 1.924617990698124 1107 60-61 1.5356490512910348 1107 62-63 -0.754405896738696 1107 64-65 1.031077195355362 1107 66-67 3.3287083884781303 1107 68-69 4.210691094459449 1107 70-71 3.3779365049355263 1107 72-73 1.505203176459549 1107 74-75 1.9534992797160022 1107 76-77 2.526054436012167 1107 78-79 3.3470434803737845 1107 80-81 1.2876203679248235 1107 82-83 0.6947131946080383 1107 84-85 1.6963334622858568 1107 86-87 1.4084419190070214 1107 88-89 2.008783443609211 1107 90-91 0.9401145426333919 1107 92-93 1.77913947341235 1107 94-95 1.593637494370281 1107 96-97 1.459973121790732 1107 98-99 1.1909476594902948 1107 100 -1.03683456555396 1108 1 4.485588941609208 1108 2 -2.8699416404262905 1108 3 0.12373492581393464 1108 4 6.603147192266562 1108 5 6.758585668625489 1108 6 6.623577660342658 1108 7 4.188477054102357 1108 8 5.01796001080012 1108 9 4.454536572637657 1108 10-11 2.9962009961594873 1108 12-13 2.4476358084470746 1108 14-15 3.7356305715649754 1108 16-17 4.490436456864206 1108 18-19 3.7390325988007227 1108 20-21 4.786602874679112 1108 22-23 4.352264808402214 1108 24-25 3.0264677446157897 1108 26-27 4.659840923025637 1108 28-29 6.374658730894296 1108 30-31 4.549440348981655 1108 32-33 4.579020403365501 1108 34-35 3.1065297888742514 1108 36-37 1.0941504591113258 1108 38-39 2.290425668698557 1108 40-41 5.307214455685976 1108 42-43 2.7855099130397996 1108 44-45 3.5403652306856124 1108 46-47 3.9849729821383058 1108 48-49 4.253011011084126 1108 50-51 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-16.67020839245542 2213 46-47 -16.445670139802843 2213 48-49 -6.200197717552303 2213 50-51 -22.695673309522494 2213 52-53 -17.19435513904852 2213 54-55 -16.824100429628412 2213 56-57 -16.978693727177905 2213 58-59 -17.24390542901013 2213 60-61 -18.39479085409809 2213 62-63 -16.48384166070391 2213 64-65 -16.528885523036603 2213 66-67 -8.608633890096375 2213 68-69 -21.708603896264115 2213 70-71 -16.030916997689133 2213 72-73 -8.737127275755807 2213 74-75 -4.112864620432596 2213 76-77 -2.9407658121215547 2213 78-79 -4.516614865342785 2213 80-81 -3.1950841313673557 2213 82-83 -2.6002291683776555 2213 84-85 -2.972185561006352 2213 86-87 -3.528227707913114 2213 88-89 -2.4739913983396313 2213 90-91 -2.074666208098609 2213 92-93 -3.507581591575473 2213 94-95 -3.3040447150925623 2213 96-97 -3.2489093971798013 2213 98-99 -3.509810282635282 2213 100 -2.9115630868633886 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 61.0 3 96.0 4 678.0 5 1839.0 6 2254.0 7 2365.0 8 2182.0 9 2276.0 10 2690.0 11 3361.0 12 4248.0 13 5275.0 14 5272.0 15 5620.0 16 5680.0 17 5623.0 18 5505.0 19 5652.0 20 5576.0 21 5811.0 22 6130.0 23 6668.0 24 7231.0 25 7859.0 26 7965.0 27 8121.0 28 8874.0 29 9518.0 30 10107.0 31 11312.0 32 13151.0 33 15037.0 34 16671.0 35 17632.0 36 16400.0 37 10025.0 38 2236.0 39 2.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.82874694942355 21.27453194966715 11.08926064213804 22.807460458771263 2 12.369109591649124 27.426470188264606 32.68003720687348 27.524383013212795 3 22.197496763803084 34.112762521592686 25.0990341196895 18.59070659491473 4 14.030643866106892 25.305711098296424 26.848144175994744 33.81550085960194 5 11.023118934019859 43.99593308241571 31.558720786519473 13.422227197044956 6 30.613932429928926 32.107306008286976 18.495691583337475 18.78306997844662 7 21.87241093620547 37.69500608154559 21.077403091893036 19.355179890355902 8 26.792326567031004 36.66517956725407 20.498773142984607 16.043720722730313 9 26.61683666484118 22.438903340635267 18.11207899780942 32.83218099671413 10-11 21.57367324908133 34.2642488741563 21.60638541473574 22.555692462026627 12-13 26.19082667705401 31.507848257213578 22.082692616908574 20.218632448823836 14-15 27.086186919709696 30.19587236746351 20.538126845441333 22.17981386738546 16-17 20.86696648003911 34.15638224214096 27.096463072298082 17.880188205521847 18-19 23.782429796090394 32.60446402579176 26.59580014859809 17.017306029519748 20-21 21.530296700551936 32.47106047846086 25.6623984049831 20.3362444160041 22-23 19.44393488474479 33.634321255689386 21.403521645016973 25.518222214548853 24-25 21.35295603299559 34.877379530579134 19.016553442319793 24.753110994105484 26-27 23.93289858412759 29.779416749142477 20.9208367792405 25.366847887489435 28-29 23.600182485716147 28.827963763550652 25.297734135691165 22.274119615042036 30-31 25.97117282942687 31.919613036480342 22.8946347194154 19.214579414677385 32-33 18.907530150781852 32.048464797076015 25.990141616461937 23.053863435680196 34-35 16.265168362254638 33.21980773608363 26.639846609115903 23.875177292545832 36-37 23.735575863265623 31.658565650965905 22.961195880158805 21.64466260560967 38-39 26.14245125469018 28.366191684559826 22.402238271845924 23.08911878890407 40-41 21.328812715792093 32.44824322462818 22.64856049732718 23.574383562252546 42-43 25.70445210247842 30.13240566694514 22.8624947786132 21.300647451963243 44-45 27.538537196772538 28.132806447962817 22.17488184247404 22.153774512790605 46-47 21.837493752339494 30.638094393225046 28.149199542162783 19.375212312272676 48-49 24.192525845090067 29.69522658303818 27.408686317106362 18.703561254765386 50-51 20.82433613181708 30.471587727345028 26.78308010432759 21.9209960365103 52-53 19.10312129593046 31.551131560053193 22.366526044786582 26.97922109922977 54-55 20.99665547556491 33.24077374409586 20.11474258687333 25.647828193465905 56-57 23.715656735690573 28.277286035124206 21.519435080736233 26.487622148448985 58-59 23.271317273431123 26.88426786381079 25.713423780159783 24.130991082598303 60-61 25.369514987091595 29.850287924129503 23.463010379670163 21.31718670910874 62-63 19.265100201259386 30.578887200697196 25.90854087989851 24.247471718144904 64-65 16.746139366892248 31.929987628148403 26.627704883592124 24.696168121367226 66-67 22.976049274819484 30.254489460432403 23.317934952244208 23.4515263125039 68-69 25.876578441076532 27.402636912489985 22.503095822674705 24.217688823758778 70-71 21.516107207910487 30.924798334634403 22.46848816029144 25.090606297163674 72-73 25.34011819995183 29.211362181367555 23.033345890277015 22.4151737284036 74-75 26.904199236502453 27.553927886451696 22.147147913862444 23.394724963183407 76-77 21.915207286342003 30.100654696364828 27.10192800185357 20.882210015439597 78-79 24.352093268865822 29.303495107179987 26.481666364131424 19.86274525982277 80-81 21.347355687069786 29.931041339981316 25.912630268480985 22.808972704467916 82-83 19.737576373288306 30.935857680459684 22.47505667311926 26.851509273132752 84-85 21.34871624203429 31.964731066162123 20.46473312050356 26.221819571300024 86-87 23.53093082223953 28.131259010433297 21.88307634747281 26.45473381985437 88-89 23.515593456743296 26.715035542559306 25.58207823033536 24.18729277036203 90-91 25.24521251751518 29.32965091485084 23.455110371017202 21.97002619661678 92-93 20.094813513771744 30.076456609197155 25.228444867283446 24.600285009747658 94-95 18.14746380428339 30.99477869021111 25.91342363503391 24.94433387047159 96-97 23.42692611000055 29.67113132535767 23.255960859350004 23.64598170529177 98-99 25.363304838437394 27.523099984338995 22.63259482535944 24.481000351864168 100 21.528046500450095 30.34708333503053 24.863647287405858 23.261222877113518 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 2609.0 1 2557.0 2 2130.0 3 1401.0 4 837.5 5 511.5 6 362.0 7 319.5 8 312.5 9 315.0 10 338.5 11 359.5 12 392.0 13 467.5 14 544.0 15 596.0 16 666.5 17 780.5 18 849.0 19 865.5 20 909.5 21 947.0 22 1027.0 23 1131.0 24 1257.5 25 1447.0 26 1692.0 27 1940.5 28 2187.5 29 2435.5 30 2648.0 31 2813.0 32 3010.0 33 3190.5 34 3343.0 35 3591.5 36 3820.0 37 4004.0 38 4119.5 39 4229.0 40 4307.0 41 4303.5 42 4331.0 43 4373.5 44 4522.5 45 5078.0 46 6379.0 47 8737.5 48 12452.5 49 18910.5 50 27260.5 51 26392.5 52 16243.0 53 9002.5 54 5852.0 55 4225.0 56 3258.0 57 2593.5 58 2116.5 59 1756.0 60 1508.0 61 1330.5 62 1122.0 63 958.5 64 831.0 65 674.5 66 592.0 67 505.0 68 377.5 69 294.0 70 240.5 71 190.5 72 134.0 73 94.0 74 92.0 75 76.0 76 53.0 77 40.5 78 28.5 79 28.5 80 23.5 81 14.0 82 13.5 83 11.0 84 8.5 85 6.5 86 2.5 87 1.5 88 0.5 89 1.0 90 1.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.5 96 0.5 97 0.0 98 0.0 99 0.0 100 0.5 >>END_MODULE >>Per base N content fail #Base N-Count 1 8.593417893709793 2 25.572968749367416 3 6.486560891972972 4 24.87905005202366 5 13.194171730707724 6 10.401493099274099 7 8.132289891215896 8 9.25211434678931 9 5.374428650664162 10-11 7.178252895713817 12-13 10.615862965227144 14-15 7.928851066586236 16-17 8.49119241466703 18-19 6.822589199321466 20-21 8.420343072756202 22-23 6.20296109763849 24-25 5.42341590992822 26-27 10.415258114273916 28-29 6.820969785792075 30-31 4.541847669866358 32-33 9.44745610377202 34-35 7.51691275004757 36-37 7.253555624830467 38-39 6.667125500499995 40-41 8.89058027635292 42-43 6.954976255349125 44-45 6.973599510937115 46-47 6.443848860135303 48-49 4.210880029797209 50-51 7.098496779391343 52-53 4.704396302878912 54-55 5.157629664417032 56-57 4.999939271992647 58-59 4.0463071298729165 60-61 4.419581948397388 62-63 3.1414598203260686 64-65 4.610267891483099 66-67 2.7198050226110615 68-69 5.515722481103468 70-71 2.7592782273899505 72-73 1.6637449747573914 74-75 0.8941186949146366 76-77 1.2750857277037122 78-79 1.865159532475314 80-81 2.2779075557786745 82-83 0.9710408375606774 84-85 1.4639498305688594 86-87 0.44837512094994797 88-89 0.7303555017550394 90-91 0.3190244652898953 92-93 0.4236790646267454 94-95 0.6342028234474886 96-97 0.6605182933000814 98-99 0.47347603065549815 100 0.6072800735213743 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 247003.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 66.07822867823366 #Duplication Level Percentage of deduplicated Percentage of total 1 94.46412907814891 62.42022323116105 2 2.839442742828829 3.7525069375878872 3 0.830071605262704 1.6454898405557237 4 0.42237134051198166 1.1163820010193126 5 0.2648670059301644 0.8750971293586235 6 0.19591501755062213 0.7767430398726103 7 0.13503654454615666 0.6246082969307598 8 0.1037514276372361 0.5484568448885199 9 0.09036025151312875 0.5373760826616507 >10 0.5478038480923761 6.998226566032567 >50 0.05789732668256883 2.643553138301448 >100 0.03244795231660451 4.291990138186941 >500 0.008907281028087511 3.8106179461775325 >1k 0.00636234359149108 7.766698580944807 >5k 6.36234359149108E-4 2.192030226320571 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 5214 2.110905535560297 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 4197 1.6991696457128052 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2305 0.9331870463111784 No Hit CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC 1974 0.7991805767541285 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 1894 0.7667923061663219 No Hit GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC 1609 0.6514090921972607 No Hit ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC 1434 0.5805597502864338 No Hit ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG 1409 0.5704384157277442 No Hit GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT 1255 0.5080909948462164 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG 1200 0.48582405881709934 No Hit TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG 1197 0.48460949867005665 No Hit GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT 937 0.3793476192596851 No Hit GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC 827 0.334813747201451 No Hit CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC 741 0.2999963563195589 No Hit ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA 708 0.2866361947020886 No Hit GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC 707 0.2862313413197411 No Hit GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT 653 0.2643692586729716 No Hit GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG 603 0.24412658955559244 No Hit CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT 582 0.2356246685262932 No Hit CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTC 571 0.2311712813204698 No Hit TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC 567 0.22955186779107947 No Hit CTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT 563 0.22793245426168912 No Hit CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA 546 0.2210499467617802 No Hit GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA 539 0.21821597308534715 No Hit GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG 520 0.21052375882074306 No Hit ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC 489 0.197973303967968 No Hit GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG 471 0.19068594308571152 No Hit CNCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 468 0.18947138293866878 No Hit CCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA 371 0.15020060485095324 No Hit AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG 359 0.14534236426278224 No Hit ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA 333 0.13481617632174508 No Hit CCCNTGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 318 0.12874337558653134 No Hit GTANATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 309 0.1250996951454031 No Hit GNACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 306 0.12388513499836035 No Hit TTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTG 300 0.12145601470427483 No Hit TGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATG 297 0.1202414545572321 No Hit ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG 282 0.11416865382201835 No Hit ANAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 264 0.10688129293976187 No Hit GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 257 0.10404731926332879 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 2.0242669117379142E-4 0.0 20-21 0.0 0.0 0.0 4.0485338234758284E-4 0.0 22-23 0.0 0.0 0.0 8.097067646951657E-4 0.0 24-25 0.0 0.0 0.0 0.0014169868382165399 0.0 26-27 0.0 0.0 0.0 0.0036436804411282457 0.0 28-29 0.0 0.0 0.0 0.008097067646951657 0.0 30-31 0.0 0.0 0.0 0.019028108970336393 0.0 32-33 0.0 0.0 0.0 0.047570272425840984 0.0 34-35 0.0 0.0 0.0 0.09979635874867918 0.0 36-37 0.0 0.0 0.0 0.16518017999781381 0.0 38-39 0.0 0.0 0.0 0.2619401383788861 0.0 40-41 0.0 0.0 0.0 0.4506018145528597 0.0 42-43 0.0 0.0 0.0 0.5659850285219208 0.0 44-45 0.0 0.0 0.0 0.6866313364615004 0.0 46-47 0.0 0.0 0.0 0.8360222345477586 0.0 48-49 0.0 0.0 0.0 0.9714456909430249 0.0 50-51 0.0 0.0 0.0 1.101403626676599 0.0 52-53 0.0 0.0 0.0 1.2987696505710458 0.0 54-55 0.0 0.0 0.0 1.4989696481419255 0.0 56-57 0.0 0.0 0.0 1.6819633769630329 0.0 58-59 0.0 0.0 0.0 1.8979526564454683 0.0 60-61 0.0 0.0 0.0 2.102808467913345 0.0 62-63 0.0 0.0 0.0 2.3066521459253533 0.0 64-65 0.0 0.0 0.0 2.597539301142091 0.0 66-67 0.0 0.0 0.0 2.882353655623616 0.0 68-69 0.0 0.0 0.0 3.2572478876774777 0.0 70-71 0.0 0.0 0.0 3.8835560701691882 0.0 72-73 0.0 0.0 0.0 4.3647243150892905 0.0 74-75 0.0 0.0 0.0 4.748120468172451 0.0 76-77 0.0 0.0 0.0 5.22483532588673 0.0 78-79 0.0 0.0 0.0 5.659647858528034 0.0 80-81 0.0 0.0 0.0 6.169560693594814 0.0 82-83 0.0 0.0 0.0 6.9161103306437575 0.0 84-85 0.0 0.0 0.0 7.573389796885058 0.0 86-87 0.0 0.0 0.0 8.164070881730181 0.0 88 0.0 0.0 0.0 8.695036092679036 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTGTATA 25 0.0016877134 75.02127 1 CTGTGCG 60 1.9826984E-10 67.0268 9 TGTATAT 40 1.3880507E-4 62.479855 2 TTAGACT 35 0.009567791 48.408394 4 ACTGTGC 95 2.1827873E-10 47.624306 8 TACTGTG 105 1.2732926E-11 47.563374 7 GTACAAA 85 2.463912E-6 44.13016 1 TGTACTG 100 1.8380888E-8 40.186707 5 CTAAGTA 65 0.0015213934 38.449146 2 TACATAA 70 0.0021891755 35.702778 2 CATGATC 65 0.002502203 34.745235 3 AGTACTC 165 4.0017767E-11 33.48892 5 AAGTACT 205 1.2551027E-10 30.304441 4 TAAGTAC 75 0.0050428947 30.112535 3 TACAAGA 105 4.4203532E-4 29.752316 2 ACATAAA 100 6.013028E-4 28.230501 3 TGTGCGT 90 3.6270649E-9 27.941452 10-11 TTTTAGA 90 0.0074898098 27.768827 2 GTACATA 205 4.7568392E-8 27.446806 1 AAAAGTA 230 4.8530637E-9 27.165157 2 >>END_MODULE