FastQCFastQC Report
Wed 25 May 2016
SRR1295082_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295082_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2307555
Sequences flagged as poor quality0
Sequence length100
%GC44

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGTACG1804.4839105E-420.597191
GTATAGA7800.018.2205921
CATGGGG40400.018.019324
GTATAGC4301.2360033E-817.244161
GTATTAG8000.016.9926831
GTACATG85300.016.5163881
TACATGG85500.015.8248052
GTCTAGC4001.8961491E-615.4478941
TAATACT11250.015.4306994
ACATGGG86750.015.2332983
GTGTATA9450.015.039221
GTATAGT5201.2778946E-714.2595941
TACATCG2600.005261162814.2573312
GTCCTAT6104.087269E-914.1816731
GTACAGG9600.014.1605691
TAGCGTG2200.00684310813.7030289
GTATCAA58350.013.3431671
TATATAG8250.013.3191015
GTATATA10800.013.1593171
GTCTAGG6651.373337E-813.0087531
Produced by FastQC (version 0.11.5)