##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1295082_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2307555 Sequences flagged as poor quality 0 Sequence length 100 %GC 44 >>END_MODULE >>Per base sequence quality warn #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 26.459795324488475 33.0 27.0 33.0 15.0 33.0 2 22.760000953390062 27.0 6.0 33.0 2.0 33.0 3 25.042688473297495 33.0 15.0 33.0 15.0 33.0 4 25.22430364606694 33.0 6.0 37.0 2.0 37.0 5 27.04587366281627 33.0 15.0 37.0 2.0 37.0 6 27.856579799831422 33.0 15.0 37.0 6.0 37.0 7 27.58356312200576 33.0 15.0 37.0 6.0 37.0 8 28.370248596458158 33.0 22.0 37.0 6.0 37.0 9 29.450842558465563 33.0 27.0 37.0 15.0 37.0 10-11 28.94940445623181 35.0 24.5 37.0 10.5 37.0 12-13 27.75608122016593 33.0 15.0 37.0 4.0 37.0 14-15 29.528618602806866 33.0 27.0 37.0 10.5 40.0 16-17 29.96275603398402 35.0 27.0 38.5 6.0 40.0 18-19 30.124743722251473 37.0 27.0 40.0 10.5 40.0 20-21 29.645436186786448 33.0 27.0 40.0 6.0 40.0 22-23 30.397106461167773 35.0 27.0 40.0 15.0 40.0 24-25 30.517792641995534 37.0 27.0 40.0 15.0 40.0 26-27 29.142439508484088 33.0 22.0 38.5 4.0 40.0 28-29 29.801410800609304 33.0 27.0 38.5 10.5 40.0 30-31 30.497351309069558 33.0 27.0 40.0 15.0 40.0 32-33 29.26614923587953 33.0 24.5 37.0 6.0 40.0 34-35 28.882388718795433 33.0 22.0 37.0 6.0 40.0 36-37 29.203616381841385 33.0 22.0 37.0 6.0 40.0 38-39 29.304531852978585 33.0 24.5 37.0 6.0 40.0 40-41 28.366034179033655 33.0 22.0 37.0 6.0 40.0 42-43 28.994174786733144 33.0 22.0 37.0 6.0 40.0 44-45 28.8708591994557 33.0 22.0 37.0 6.0 40.0 46-47 28.555232486332937 33.0 22.0 37.0 6.0 40.0 48-49 28.471605660536802 33.0 22.0 37.0 6.0 40.0 50-51 28.452814342453376 33.0 22.0 37.0 6.0 40.0 52-53 28.590773351014384 33.0 22.0 37.0 6.0 40.0 54-55 28.564364229671668 33.0 22.0 37.0 6.0 40.0 56-57 28.585269906892794 33.0 22.0 37.0 6.0 40.0 58-59 28.427532388177095 33.0 22.0 37.0 6.0 40.0 60-61 28.341154165339503 33.0 22.0 37.0 10.5 40.0 62-63 28.270974906340264 33.0 22.0 37.0 10.5 40.0 64-65 27.859518841371063 33.0 22.0 37.0 6.0 40.0 66-67 28.04434672196329 33.0 22.0 37.0 10.5 38.5 68-69 27.602620305908204 33.0 22.0 37.0 6.0 37.0 70-71 27.720061927017994 33.0 22.0 37.0 10.5 37.0 72-73 27.763868033481323 33.0 22.0 37.0 10.5 37.0 74-75 27.8393635254631 33.0 22.0 37.0 15.0 37.0 76-77 27.606004840621353 33.0 22.0 37.0 15.0 37.0 78-79 27.17279371455935 30.0 22.0 33.0 10.5 37.0 80-81 26.96086810498558 27.0 22.0 33.0 6.0 37.0 82-83 27.080169053392012 27.0 22.0 33.0 15.0 37.0 84-85 27.042702774148395 27.0 22.0 33.0 10.5 37.0 86-87 26.8097013938996 27.0 22.0 33.0 15.0 37.0 88-89 26.773087748721046 27.0 22.0 33.0 15.0 37.0 90-91 26.933665069738318 27.0 22.0 33.0 15.0 37.0 92-93 26.877537480146735 27.0 22.0 33.0 15.0 37.0 94-95 26.75893900687091 27.0 22.0 33.0 15.0 37.0 96-97 26.846934959296746 27.0 22.0 33.0 15.0 37.0 98-99 26.914174960076792 27.0 22.0 33.0 15.0 37.0 100 26.609204981029706 27.0 22.0 33.0 15.0 37.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 7.350278126166067 1101 2 11.29417103904725 1101 3 8.416300850157484 1101 4 12.904071704258264 1101 5 10.774851536087468 1101 6 9.95539091373865 1101 7 9.492439925660847 1101 8 9.003845955355143 1101 9 7.308179031943691 1101 10-11 7.799403014128401 1101 12-13 9.123693018811814 1101 14-15 8.024826242560803 1101 16-17 8.176760957274869 1101 18-19 8.302776432121775 1101 20-21 7.270628468746807 1101 22-23 7.507358825846676 1101 24-25 7.503825524279126 1101 26-27 8.657473938811755 1101 28-29 8.23545136753966 1101 30-31 5.510538882125257 1101 32-33 7.029514773288945 1101 34-35 8.629148634501774 1101 36-37 8.346472176852082 1101 38-39 8.269416956803237 1101 40-41 9.057308834899114 1101 42-43 8.70122838797403 1101 44-45 8.565917501926815 1101 46-47 7.38484286395758 1101 48-49 5.422303885533406 1101 50-51 6.79886845558725 1101 52-53 5.8446696983992545 1101 54-55 4.019309824279137 1101 56-57 6.170284385637796 1101 58-59 6.230751159139789 1101 60-61 6.714610653966201 1101 62-63 6.246230604822433 1101 64-65 6.757289569055228 1101 66-67 5.764477065767959 1101 68-69 6.7776618918301885 1101 70-71 6.170024023921997 1101 72-73 5.459739166614359 1101 74-75 4.815034070900548 1101 76-77 4.996881161320264 1101 78-79 5.03653927835223 1101 80-81 4.791534555389379 1101 82-83 4.476602817174747 1101 84-85 4.136386563296799 1101 86-87 4.103944077358516 1101 88-89 3.9574178764476216 1101 90-91 3.7587959945554914 1101 92-93 3.6930861197669067 1101 94-95 3.693871749295944 1101 96-97 3.7660963156425105 1101 98-99 3.4923374171489243 1101 100 3.861849554244749 1102 1 6.790539533049287 1102 2 10.587520673529163 1102 3 8.111867467243691 1102 4 12.62475366617867 1102 5 10.486316858414028 1102 6 9.601163329331914 1102 7 6.303642058505734 1102 8 7.550726974827544 1102 9 6.376891797103273 1102 10-11 7.409710823695335 1102 12-13 9.129117774135423 1102 14-15 6.223074055422753 1102 16-17 7.382230060169771 1102 18-19 7.4612666706095325 1102 20-21 7.014717846649454 1102 22-23 6.572407792180254 1102 24-25 6.99720869505904 1102 26-27 8.504084622104422 1102 28-29 7.625218285804259 1102 30-31 4.128894859738427 1102 32-33 4.277083595486705 1102 34-35 4.965022558828604 1102 36-37 5.133090386499013 1102 38-39 4.5462150559445895 1102 40-41 6.116641956742981 1102 42-43 6.983170712218442 1102 44-45 6.507049653030915 1102 46-47 5.55948033110257 1102 48-49 3.677517364896957 1102 50-51 4.877695758908647 1102 52-53 5.758142361677432 1102 54-55 5.413870387362859 1102 56-57 6.292140614879958 1102 58-59 6.0896335304677685 1102 60-61 6.078990878023912 1102 62-63 4.416286091304887 1102 64-65 6.184932259617412 1102 66-67 3.8029614914009144 1102 68-69 6.049831698463283 1102 70-71 5.2648989058520215 1102 72-73 4.985649829084156 1102 74-75 4.311738631759436 1102 76-77 3.3913190348914846 1102 78-79 4.414765657331106 1102 80-81 4.588676637026488 1102 82-83 4.075266264294694 1102 84-85 3.0772352144769073 1102 86-87 3.4084200648411986 1102 88-89 3.4990846013437675 1102 90-91 3.464562553039549 1102 92-93 3.223420922191181 1102 94-95 3.208337892891084 1102 96-97 3.349054321163287 1102 98-99 3.190776843816817 1102 100 3.426535634464475 1103 1 6.72959874569532 1103 2 11.038365092054775 1103 3 8.011719922395642 1103 4 12.129743146645978 1103 5 10.499842197281204 1103 6 9.06426204884271 1103 7 7.987744688878038 1103 8 8.12059279753326 1103 9 7.12286780366966 1103 10-11 7.465629192942139 1103 12-13 8.868186777346985 1103 14-15 8.576605301764115 1103 16-17 8.035906003338102 1103 18-19 7.326953681140424 1103 20-21 8.29785938430313 1103 22-23 6.8520024390998024 1103 24-25 5.169659645193587 1103 26-27 5.923805574419546 1103 28-29 6.939842369112263 1103 30-31 6.110963253583922 1103 32-33 8.08966475619809 1103 34-35 7.250103157036101 1103 36-37 4.072666858152765 1103 38-39 6.409814096232491 1103 40-41 6.966062112274912 1103 42-43 7.551184216729677 1103 44-45 5.5925517031327345 1103 46-47 6.451431855101525 1103 48-49 5.600716571427238 1103 50-51 6.36373950893034 1103 52-53 6.110648241539227 1103 54-55 4.966503828589644 1103 56-57 5.896627131669714 1103 58-59 5.4609478677372145 1103 60-61 5.030966497385663 1103 62-63 2.7531704966775337 1103 64-65 4.989252463021341 1103 66-67 4.025869197846152 1103 68-69 5.001704915294411 1103 70-71 3.600279106409509 1103 72-73 4.293142972532589 1103 74-75 3.104848045944383 1103 76-77 3.4263975030001212 1103 78-79 2.9661406834839887 1103 80-81 4.054566811653569 1103 82-83 3.313357591573496 1103 84-85 3.2840827644056354 1103 86-87 2.885516492365607 1103 88-89 2.9927249150584174 1103 90-91 2.618590711999836 1103 92-93 2.3115081628358674 1103 94-95 2.726383301549763 1103 96-97 2.918301192363625 1103 98-99 2.777399680938096 1103 100 2.8039572018574894 1104 1 6.522749579046334 1104 2 9.966741506649353 1104 3 7.555253409076425 1104 4 12.408825407030335 1104 5 9.584836826125695 1104 6 8.353833326497519 1104 7 4.205089998708608 1104 8 6.865151233162557 1104 9 6.8193111156700645 1104 10-11 7.370724931987382 1104 12-13 7.624165659709362 1104 14-15 7.605532006747662 1104 16-17 7.529712938760671 1104 18-19 7.123953194796989 1104 20-21 7.789824514628762 1104 22-23 6.662978172295322 1104 24-25 6.309687199796283 1104 26-27 8.462857753741808 1104 28-29 7.445083922113902 1104 30-31 6.58059727650571 1104 32-33 7.924221262972807 1104 34-35 8.167250714735339 1104 36-37 6.8317322317472815 1104 38-39 7.473792307787516 1104 40-41 7.224726572392402 1104 42-43 7.469928423890284 1104 44-45 7.0905188750318935 1104 46-47 6.9774277052300135 1104 48-49 5.712502966087083 1104 50-51 5.919087229910108 1104 52-53 6.187993245279518 1104 54-55 5.081687744306958 1104 56-57 6.291392081061982 1104 58-59 4.195964020072349 1104 60-61 4.506786701902552 1104 62-63 4.133325870691504 1104 64-65 2.9329438372192946 1104 66-67 3.5478440906700186 1104 68-69 4.400591637438456 1104 70-71 2.689785489687324 1104 72-73 2.5114886631135427 1104 74-75 3.502054591611998 1104 76-77 4.0897443993176275 1104 78-79 4.5556157694362405 1104 80-81 3.213403532574876 1104 82-83 3.036039601514471 1104 84-85 3.1029895152185176 1104 86-87 0.8508280023801866 1104 88-89 0.509083658689029 1104 90-91 2.36234877824781 1104 92-93 2.5264590855522826 1104 94-95 2.5798555798930316 1104 96-97 3.0274819328397236 1104 98-99 2.6093449866550706 1104 100 0.8589470482438983 1105 1 5.792813693465362 1105 2 -2.8296472248170943 1105 3 0.22442833392901917 1105 4 8.529702129881517 1105 5 8.60276394960124 1105 6 7.988133141617812 1105 7 5.010898556396452 1105 8 5.855996738647399 1105 9 4.806511644685607 1105 10-11 5.02295872311614 1105 12-13 5.135648628595355 1105 14-15 5.888171457220206 1105 16-17 6.0688147152716745 1105 18-19 6.083922390214184 1105 20-21 6.915576998202866 1105 22-23 2.1291494198666463 1105 24-25 3.986754141945653 1105 26-27 6.79129567970525 1105 28-29 5.894631108370071 1105 30-31 5.173200652471319 1105 32-33 7.195177002054784 1105 34-35 6.023686336289849 1105 36-37 6.150520894003144 1105 38-39 4.042680087037407 1105 40-41 5.251216364035425 1105 42-43 5.52673753296229 1105 44-45 6.117625720948862 1105 46-47 5.288637629231346 1105 48-49 4.98565928598649 1105 50-51 5.925277718414943 1105 52-53 5.523828969997279 1105 54-55 5.191868764569165 1105 56-57 5.033577175155006 1105 58-59 1.9092665444720573 1105 60-61 4.372957774081666 1105 62-63 -0.9035675751136445 1105 64-65 -0.5218618318811927 1105 66-67 0.5224550483106789 1105 68-69 2.6629674138651076 1105 70-71 2.8276584928367967 1105 72-73 2.4228939577231046 1105 74-75 2.4248533846851643 1105 76-77 1.5945955235531493 1105 78-79 3.0499428103224524 1105 80-81 1.5122652126478933 1105 82-83 0.8277769927111329 1105 84-85 1.1632044351242357 1105 86-87 -0.6061325231960346 1105 88-89 1.3965932736492803 1105 90-91 1.2543038311791435 1105 92-93 1.6009174438110243 1105 94-95 0.9849193855401879 1105 96-97 1.8894704718152582 1105 98-99 1.78480362301244 1105 100 1.9729000615677528 1106 1 6.190625576170014 1106 2 10.332870262614996 1106 3 6.815445475241432 1106 4 11.820674993892375 1106 5 10.029262558181884 1106 6 9.394500688751283 1106 7 6.5116029319568085 1106 8 7.428722960003288 1106 9 6.836840922016112 1106 10-11 5.8904555667124825 1106 12-13 8.06446370470718 1106 14-15 8.0203804099129 1106 16-17 7.89705529299923 1106 18-19 7.3414106837382604 1106 20-21 8.422217542425003 1106 22-23 6.317061966405607 1106 24-25 5.094068871937804 1106 26-27 7.896828728205385 1106 28-29 6.37121593553352 1106 30-31 5.351469103799328 1106 32-33 7.361464782622122 1106 34-35 6.864281486838621 1106 36-37 6.019905947962833 1106 38-39 7.012129482690316 1106 40-41 6.6114829670121615 1106 42-43 6.31131979502474 1106 44-45 4.448060571325772 1106 46-47 6.118971304943024 1106 48-49 5.920486104387919 1106 50-51 6.650532144340534 1106 52-53 5.688943108583647 1106 54-55 5.319980817553439 1106 56-57 4.9001882744163545 1106 58-59 4.969897356413224 1106 60-61 4.9663398880666065 1106 62-63 5.167710432639403 1106 64-65 5.322393691934277 1106 66-67 3.1511468824959117 1106 68-69 3.4754017009002105 1106 70-71 3.106443874918103 1106 72-73 2.8909824926544587 1106 74-75 2.9115815768648368 1106 76-77 3.5606706191891853 1106 78-79 4.226290985541585 1106 80-81 3.811796021582772 1106 82-83 2.2270696505239798 1106 84-85 2.7390021860562435 1106 86-87 3.450581389514223 1106 88-89 3.363850498674509 1106 90-91 3.233939147871709 1106 92-93 3.01838560869297 1106 94-95 1.4945563219792568 1106 96-97 1.5119996345895501 1106 98-99 1.8586613467641193 1106 100 2.824640230114987 1107 1 6.0251055717720945 1107 2 10.400299485595383 1107 3 6.1443363969810285 1107 4 11.53126088598755 1107 5 9.283788439258537 1107 6 7.908654787535966 1107 7 3.654717480721299 1107 8 6.514917293412161 1107 9 5.56009578493909 1107 10-11 4.458545584822257 1107 12-13 6.078655256986444 1107 14-15 5.979610758396511 1107 16-17 6.7890652207460285 1107 18-19 6.218794745002214 1107 20-21 5.453326110495244 1107 22-23 5.733618052321628 1107 24-25 4.497399800653948 1107 26-27 7.100040019306846 1107 28-29 6.404333683862653 1107 30-31 5.0974286548161665 1107 32-33 4.973823506419016 1107 34-35 6.535107132713655 1107 36-37 6.282621092977465 1107 38-39 6.109465241997629 1107 40-41 7.516372566212308 1107 42-43 6.02832251485243 1107 44-45 3.962191909301442 1107 46-47 3.3112987766264794 1107 48-49 3.9130380399663274 1107 50-51 4.702288879344994 1107 52-53 3.7799809461050877 1107 54-55 5.124507687370706 1107 56-57 5.1135435498814665 1107 58-59 2.8208739157461657 1107 60-61 2.8710257276003333 1107 62-63 0.3391895767380646 1107 64-65 2.160051440556071 1107 66-67 3.9868889461716 1107 68-69 4.912405971073188 1107 70-71 4.060167452308214 1107 72-73 2.26589497922242 1107 74-75 2.3290142208877604 1107 76-77 3.0222461615430767 1107 78-79 3.894272182859009 1107 80-81 2.165993383701906 1107 82-83 1.6265487819667612 1107 84-85 2.311974526235222 1107 86-87 2.293725857569793 1107 88-89 2.620208456790664 1107 90-91 1.4590809431636416 1107 92-93 2.3213320161830353 1107 94-95 2.1286432338615064 1107 96-97 2.3420820876471105 1107 98-99 1.8387270505556224 1107 100 0.006741612413922127 1108 1 4.361610333631749 1108 2 -3.282171905353925 1108 3 -0.02239441111413143 1108 4 6.798946789556737 1108 5 7.015848061972729 1108 6 6.644291765207644 1108 7 4.228207492815411 1108 8 5.042807289086067 1108 9 4.835591509370143 1108 10-11 3.224095638030036 1108 12-13 2.3952735708839406 1108 14-15 3.9387541683679963 1108 16-17 4.478068123491042 1108 18-19 3.933088040663808 1108 20-21 4.6867460248891355 1108 22-23 4.1651481036663505 1108 24-25 2.902790798907727 1108 26-27 4.726115641458964 1108 28-29 6.082627632385304 1108 30-31 4.452700389484587 1108 32-33 4.6597285360543275 1108 34-35 3.043413210873833 1108 36-37 1.1517458960139386 1108 38-39 2.5339748462390936 1108 40-41 5.264960250900149 1108 42-43 3.0490581953278593 1108 44-45 3.6570485759049483 1108 46-47 4.779568386930791 1108 48-49 4.844574840962906 1108 50-51 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-17.428257012345842 2213 42-43 -23.657763571233655 2213 44-45 -17.446335751011937 2213 46-47 -17.305780547595703 2213 48-49 -6.199818949676381 2213 50-51 -23.812525935469818 2213 52-53 -18.19832567684439 2213 54-55 -18.044662503228057 2213 56-57 -17.917960658408454 2213 58-59 -18.33586879635369 2213 60-61 -19.698511666937062 2213 62-63 -17.848936814959757 2213 64-65 -17.95249886485138 2213 66-67 -8.706052719984353 2213 68-69 -23.12663306796585 2213 70-71 -17.758558161060254 2213 72-73 -9.500772198159044 2213 74-75 -4.311282964052282 2213 76-77 -3.0607151160610577 2213 78-79 -4.512796875995765 2213 80-81 -3.242270492419113 2213 82-83 -3.2147677897069507 2213 84-85 -3.023823772063075 2213 86-87 -3.377068406081616 2213 88-89 -2.6709589861624927 2213 90-91 -2.9231786123102026 2213 92-93 -3.724383342032322 2213 94-95 -3.7221790169607445 2213 96-97 -3.419048430626262 2213 98-99 -3.794200009157155 2213 100 -3.211081312551862 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 634.0 3 557.0 4 2756.0 5 10089.0 6 16902.0 7 19688.0 8 17998.0 9 18474.0 10 20766.0 11 24935.0 12 30790.0 13 41341.0 14 32371.0 15 36058.0 16 41636.0 17 44252.0 18 45452.0 19 46595.0 20 46912.0 21 45988.0 22 48580.0 23 53302.0 24 58915.0 25 64634.0 26 70615.0 27 76304.0 28 81924.0 29 86221.0 30 90939.0 31 99950.0 32 114036.0 33 137660.0 34 164356.0 35 190542.0 36 204186.0 37 170812.0 38 50348.0 39 37.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.98709958713235 20.437957917254696 14.13953208958341 24.435410406029547 2 15.279591200633675 21.24961753696666 39.84970275116026 23.62108851123941 3 17.875414988595875 26.25205882244568 30.118177329555294 25.75434885940315 4 12.811641651358055 18.089621929039094 38.477445183956995 30.62129123564586 5 13.592657881740466 37.56964518390571 35.494979725006054 13.342717209347763 6 30.30796904557509 37.32375315938427 18.704416650427866 13.66386114461278 7 25.988785660660664 32.0083521021021 23.553866366366368 18.44899587087087 8 24.03107542313577 35.56152815172398 22.285822727757946 18.12157369738231 9 24.76522992126847 17.47977836097429 21.870782402562654 35.88420931519458 10-11 23.21497380987021 27.22251221656023 29.242724331278996 20.319789642290566 12-13 24.262033076669418 25.058116444703327 28.573481378427235 22.10636910020002 14-15 21.76627676763612 26.641480845783132 26.122189991900047 25.470052394680703 16-17 21.488155315701903 29.614896411763976 26.96761928675395 21.929328985780174 18-19 21.603925899368335 28.944393914586968 28.244986992362392 21.20669319368231 20-21 22.727098674002388 28.189212776278865 27.59078132726617 21.492907222452573 22-23 22.289871480964855 28.05924236354405 27.532474517183104 22.118411638307993 24-25 22.328159467739482 28.150502196215033 27.29747192264017 22.223866413405315 26-27 22.168781378235117 27.84474882351788 27.499465724365407 22.487004073881593 28-29 22.130558786802037 27.90639486460917 27.786488987257403 22.176557361331394 30-31 22.451833096037518 28.227111885636614 27.378320397978445 21.94273462034743 32-33 22.016186965817546 27.946493560016695 27.610594552401334 22.426724921764425 34-35 21.989863019124204 27.679689110841633 27.571190099261333 22.75925777077283 36-37 22.499160251843087 27.987823942295066 27.225878887975853 22.287136917885995 38-39 22.314448974778138 27.805248732365236 27.42633460121764 22.45396769163899 40-41 22.240399320376163 27.82795786370029 27.342920912050083 22.588721903873463 42-43 22.300661663480057 27.80607319001073 27.531989199636776 22.361275946872436 44-45 22.483410182274604 27.72911667707229 27.525807989529454 22.26166515112366 46-47 22.302870112333725 27.847157181026805 27.67475507800155 22.175217628637917 48-49 22.34771772883373 27.731012352365607 27.575241918423067 22.3460280003776 50-51 22.235732827649205 27.779218001121603 27.668214168020487 22.316835003208706 52-53 22.140719412054338 27.67183773861468 27.45691718797153 22.730525661359454 54-55 22.32958623974259 27.993295794033667 27.29443587365466 22.382682092569087 56-57 22.40343887388503 27.67706893025298 27.320827855859136 22.598664340002856 58-59 22.278373525149377 27.371710604562526 27.631123639932913 22.718792230355184 60-61 22.409699050315258 27.733185274169326 27.40886520062785 22.448250474887566 62-63 22.218131224108156 27.739949715021968 27.477854925154123 22.564064135715757 64-65 22.15823402548001 27.594813534194785 27.55368312087152 22.693269319453687 66-67 22.30205533354456 27.59436257464821 27.300102337862477 22.803479753944764 68-69 22.74103839859903 27.54312816670862 27.22038432144493 22.495449113247425 70-71 22.531763059566725 27.63214484084075 27.280280175934557 22.55581192365797 72-73 22.542235126559657 27.649273306168237 27.32153246339036 22.48695910388175 74-75 22.595627901813323 27.608173744071813 27.167484918110024 22.62871343600484 76-77 22.56504035113551 27.625606494043737 27.284913723592968 22.524439431227783 78-79 22.624147861128403 27.58102250711553 27.373459899340734 22.42136973241533 80-81 22.53470186118192 27.475027114811223 27.393937540417358 22.5963334835895 82-83 22.342590506529184 27.567029583483965 27.44929556064361 22.64108434934324 84-85 22.46640690433706 27.567990179281637 27.119687372628782 22.845915543752522 86-87 22.58218965025996 27.562340929230334 27.219889498911282 22.635579921598424 88-89 22.52546923559886 27.262311685437833 27.447109994930486 22.76510908403282 90-91 22.51767977371281 27.378498021378334 27.35982480655071 22.743997398358147 92-93 22.40864620824047 27.47878925954364 27.396398456705047 22.716166075510845 94-95 22.254988331610935 27.38777274791379 27.424601951664663 22.93263696881061 96-97 22.492125344386828 27.284913732815518 27.387592531833043 22.835368390964614 98-99 22.514646423421215 27.29168231164803 27.327967784942725 22.865703479988028 100 22.44167396947701 27.20535444665003 27.343244849669436 23.00972673420352 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 370.0 1 420.0 2 404.5 3 324.0 4 319.5 5 382.5 6 536.5 7 784.0 8 1103.0 9 1630.0 10 2297.5 11 2897.5 12 3570.5 13 4314.5 14 5209.5 15 6072.0 16 6708.5 17 7332.0 18 7932.0 19 8574.5 20 9220.0 21 9865.0 22 10829.0 23 12133.0 24 13690.5 25 15798.5 26 18861.5 27 22869.5 28 27895.5 29 33509.5 30 39095.5 31 44967.0 32 50953.0 33 56851.0 34 62091.0 35 66512.5 36 70845.0 37 74833.5 38 77812.0 39 79873.0 40 81192.5 41 81364.5 42 80639.0 43 79245.0 44 77237.0 45 75171.5 46 73553.0 47 72059.0 48 70095.0 49 69158.0 50 69219.5 51 66766.5 52 61456.0 53 56660.0 54 52932.0 55 49253.0 56 45474.0 57 41746.0 58 38110.0 59 34436.5 60 30867.5 61 26836.5 62 22758.5 63 19096.5 64 15735.5 65 12822.5 66 10230.5 67 8041.5 68 6311.0 69 4859.0 70 3691.0 71 2868.5 72 2255.5 73 1705.0 74 1251.0 75 933.5 76 652.0 77 440.5 78 302.5 79 192.5 80 135.5 81 87.5 82 55.0 83 41.5 84 26.0 85 22.5 86 19.0 87 11.5 88 9.5 89 6.5 90 2.5 91 3.0 92 3.5 93 2.0 94 1.0 95 0.5 96 0.0 97 0.0 98 0.0 99 0.5 100 3.0 >>END_MODULE >>Per base N content fail #Base N-Count 1 7.66386933355868 2 24.225641425664826 3 5.911495067289837 4 23.968919484042633 5 12.163263714190995 6 9.487791190242486 7 7.642504729031377 8 8.657648463416907 9 5.084472526115304 10-11 6.767856020766569 12-13 9.978527055693148 14-15 7.38903298079569 16-17 7.836931297412196 18-19 6.565997343508606 20-21 7.76395362190717 22-23 5.725475665802115 24-25 5.261304714297167 26-27 9.357870126605865 28-29 6.212527978748069 30-31 4.198491476909543 32-33 8.728546015154569 34-35 6.996691303132536 36-37 6.786945489923317 38-39 5.931754606065728 40-41 8.344828183943612 42-43 6.413628277549181 44-45 6.329794956133224 46-47 5.725432329890295 48-49 3.824979252932216 50-51 6.303966752688452 52-53 4.504768033698005 54-55 4.914725759516025 56-57 4.549122339445864 58-59 3.4429515222822427 60-61 4.1137047654335435 62-63 3.132969745033163 64-65 4.383795835852233 66-67 2.477340735107072 68-69 5.048568723172362 70-71 2.5121178043426915 72-73 1.4915137450678315 74-75 0.8470437324354133 76-77 1.1088359757405566 78-79 1.7250076379544583 80-81 2.2278125548470133 82-83 0.746374409277352 84-85 1.226059617213891 86-87 0.5283947728223163 88-89 0.7110773090998914 90-91 0.323675925384227 92-93 0.3267961110352733 94-95 0.5123388174929742 96-97 0.5431723187529658 98-99 0.45470205477225895 100 0.4053207832532702 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 2307555.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 93.53730278581625 #Duplication Level Percentage of deduplicated Percentage of total 1 95.89177041545476 89.6945756401837 2 3.1222090342665134 5.840860235975956 3 0.5691469887850366 1.597094226588646 4 0.18464947814536178 0.6908645658610266 5 0.08230322417307251 0.3849210799862797 6 0.04685235503669675 0.262946575157764 7 0.026892311033993595 0.1760803967857904 8 0.016948341022986143 0.12682416847874606 9 0.012588518276930636 0.10597464411246384 >10 0.044460965472690406 0.6357361138142485 >50 6.39298216944923E-4 0.042435680799247345 >100 0.0013527471538091783 0.2629174520057399 >500 9.316148057913058E-5 0.051543745443992046 >1k 9.316148057883973E-5 0.1272254748064169 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 4.333591182008663E-5 0.0 0.0 0.0 3 0.0 4.333591182008663E-5 0.0 0.0 0.0 4 0.0 4.333591182008663E-5 0.0 0.0 0.0 5 0.0 4.333591182008663E-5 0.0 0.0 0.0 6 0.0 4.333591182008663E-5 0.0 0.0 0.0 7 0.0 4.333591182008663E-5 0.0 0.0 0.0 8 0.0 4.333591182008663E-5 0.0 4.333591182008663E-5 0.0 9 0.0 4.333591182008663E-5 0.0 8.667182364017326E-5 0.0 10-11 0.0 8.667182364017326E-5 0.0 8.667182364017326E-5 0.0 12-13 0.0 8.667182364017326E-5 0.0 8.667182364017326E-5 0.0 14-15 0.0 1.0833977955021657E-4 0.0 1.0833977955021657E-4 0.0 16-17 0.0 1.3000773546025988E-4 0.0 1.516756913703032E-4 0.0 18-19 0.0 1.3000773546025988E-4 0.0 1.7334364728034652E-4 0.0 20-21 0.0 1.3000773546025988E-4 0.0 2.1667955910043314E-4 0.0 22-23 0.0 1.3000773546025988E-4 0.0 2.6001547092051975E-4 0.0 24-25 0.0 1.3000773546025988E-4 0.0 3.250193386506497E-4 0.0 26-27 0.0 1.3000773546025988E-4 0.0 4.983629859309963E-4 0.0 28-29 2.1667955910043315E-5 1.3000773546025988E-4 0.0 9.533900600419058E-4 0.0 30-31 4.333591182008663E-5 1.3000773546025988E-4 0.0 0.002405143106014808 0.0 32-33 4.333591182008663E-5 1.3000773546025988E-4 0.0 0.0067604022439335144 0.0 34-35 4.333591182008663E-5 1.7334364728034652E-4 2.1667955910043315E-5 0.014279182944718544 0.0 36-37 4.333591182008663E-5 2.1667955910043314E-4 4.333591182008663E-5 0.02537317637066072 0.0 38-39 8.667182364017326E-5 2.1667955910043314E-4 4.333591182008663E-5 0.04136412783227268 0.0 40-41 8.667182364017326E-5 2.1667955910043314E-4 4.333591182008663E-5 0.062057025726364054 0.0 42-43 8.667182364017326E-5 2.6001547092051975E-4 4.333591182008663E-5 0.0836383098127672 0.0 44-45 8.667182364017326E-5 2.6001547092051975E-4 4.333591182008663E-5 0.11013821989075016 0.0 46-47 8.667182364017326E-5 2.6001547092051975E-4 4.333591182008663E-5 0.14220679463761426 0.0 48-49 8.667182364017326E-5 2.6001547092051975E-4 4.333591182008663E-5 0.17596546994546175 0.0 50-51 8.667182364017326E-5 2.6001547092051975E-4 4.333591182008663E-5 0.2158995126876716 2.1667955910043315E-5 52-53 8.667182364017326E-5 2.816834268305631E-4 4.333591182008663E-5 0.2559635631653417 4.333591182008663E-5 54-55 8.667182364017326E-5 3.033513827406064E-4 4.333591182008663E-5 0.3057565258466212 6.500386773012994E-5 56-57 8.667182364017326E-5 3.033513827406064E-4 4.333591182008663E-5 0.36339328856733644 8.667182364017326E-5 58-59 8.667182364017326E-5 3.033513827406064E-4 4.333591182008663E-5 0.42716208281059387 8.667182364017326E-5 60-61 8.667182364017326E-5 3.033513827406064E-4 4.333591182008663E-5 0.493271016292136 8.667182364017326E-5 62-63 8.667182364017326E-5 3.033513827406064E-4 4.333591182008663E-5 0.5651652940016598 8.667182364017326E-5 64-65 8.667182364017326E-5 3.033513827406064E-4 4.333591182008663E-5 0.6485652562994164 8.667182364017326E-5 66-67 8.667182364017326E-5 3.033513827406064E-4 4.333591182008663E-5 0.7468294363514629 1.3000773546025988E-4 68-69 8.667182364017326E-5 3.033513827406064E-4 4.333591182008663E-5 0.852872412575215 1.516756913703032E-4 70-71 8.667182364017326E-5 3.033513827406064E-4 4.333591182008663E-5 0.9818400861517927 1.7334364728034652E-4 72-73 1.0833977955021657E-4 3.033513827406064E-4 4.333591182008663E-5 1.1174598221927539 1.7334364728034652E-4 74-75 1.3000773546025988E-4 3.033513827406064E-4 4.333591182008663E-5 1.26566864061745 1.7334364728034652E-4 76-77 1.3000773546025988E-4 3.033513827406064E-4 4.333591182008663E-5 1.4355887508640097 1.7334364728034652E-4 78-79 1.3000773546025988E-4 3.033513827406064E-4 4.333591182008663E-5 1.6149344219314383 1.7334364728034652E-4 80-81 1.3000773546025988E-4 3.033513827406064E-4 4.333591182008663E-5 1.8220800804314523 1.7334364728034652E-4 82-83 1.3000773546025988E-4 3.250193386506497E-4 4.333591182008663E-5 2.050525339591039 1.7334364728034652E-4 84-85 1.3000773546025988E-4 3.4668729456069304E-4 4.333591182008663E-5 2.2880061363651136 1.7334364728034652E-4 86-87 1.3000773546025988E-4 3.4668729456069304E-4 4.333591182008663E-5 2.5474365724760624 1.7334364728034652E-4 88 1.3000773546025988E-4 3.4668729456069304E-4 4.333591182008663E-5 2.7695114525980964 1.7334364728034652E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTGTACG 180 4.4839105E-4 20.59719 1 GTATAGA 780 0.0 18.220592 1 CATGGGG 4040 0.0 18.01932 4 GTATAGC 430 1.2360033E-8 17.24416 1 GTATTAG 800 0.0 16.992683 1 GTACATG 8530 0.0 16.516388 1 TACATGG 8550 0.0 15.824805 2 GTCTAGC 400 1.8961491E-6 15.447894 1 TAATACT 1125 0.0 15.430699 4 ACATGGG 8675 0.0 15.233298 3 GTGTATA 945 0.0 15.03922 1 GTATAGT 520 1.2778946E-7 14.259594 1 TACATCG 260 0.0052611628 14.257331 2 GTCCTAT 610 4.087269E-9 14.181673 1 GTACAGG 960 0.0 14.160569 1 TAGCGTG 220 0.006843108 13.703028 9 GTATCAA 5835 0.0 13.343167 1 TATATAG 825 0.0 13.319101 5 GTATATA 1080 0.0 13.159317 1 GTCTAGG 665 1.373337E-8 13.008753 1 >>END_MODULE