FastQCFastQC Report
Wed 25 May 2016
SRR1295082_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1295082_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2307555
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT48700.2110458905638219No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA35060.15193570684122373No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA96400.021.0623661
ACCGTAC1708.705972E-519.331518
GAGTACT44250.018.8848712-13
TATCAAC107350.018.6199682
ATCAACG106500.018.5039023
CAACGCA106300.018.4952755
TCAACGC106900.018.4794624
AACGCAG106350.018.4561126
ACGCAGA116950.016.65967
CGCAGAG118950.016.4189578
GTACTTT51950.016.2213214-15
GGTATCA74250.016.0186351
AGTACTT50450.015.54041412-13
GCAGAGT128400.014.66177759
ATAGTAC7100.014.5734783
CTAGTAC5208.913048E-1114.4715663
CATGGGG45900.014.3460764
GTACATG109400.013.9651351
ACATGGG108600.013.9452073
TATACTG11650.013.3231155
Produced by FastQC (version 0.11.5)